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Sperm apoptosis

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https://www.readbyqxmd.com/read/28323003/pulsed-or-continuous-electromagnetic-field-induce-p53-p21-mediated-apoptotic-signaling-pathway-in-mouse-spermatogenic-cells-in-vitro-and-thus-may-affect-male-fertility
#1
Przemyslaw Solek, Lena Majchrowicz, Dominika Bloniarz, Ewelina Krotoszynska, Marek Koziorowski
The impact of electromagnetic field (EMF) on the human health and surrounding environment is a common topic investigated over the years. A significant increase in the electromagnetic field concentration arouses public concern about the long-term effects of EMF on living organisms associated with many aspects. In the present study, we investigated the effects of pulsed and continuous electromagnetic field (PEMF/CEMF) on mouse spermatogenic cell lines (GC-1 spg and GC-2 spd) in terms of cellular and biochemical features in vitro...
March 16, 2017: Toxicology
https://www.readbyqxmd.com/read/28295950/sulforaphane-attenuates-di-n-butylphthalate-induced-reproductive-damage-in-pubertal-mice-involvement-of-the-nrf2-antioxidant-system
#2
Xu-Ping Jiang, Jing-Yuan Tang, Zhen Xu, Peng Han, Zhi-Qiang Qin, Cheng-di Yang, Shang-Qian Wang, Min Tang, Wei Wang, Chao Qin, Yang Xu, Bai-Xin Shen, Wei-Min Zhou, Wei Zhang
di-N-butylphthalate (DBP) is a ubiquitous environmental pollutant used for plastic coating and in the cosmetics industry. It has toxic effects on body health, especially the male reproductive system. Here, we investigated the effects of DBP on the male reproductive system of pubertal mice and explored the protective role of sulforaphane (SFN). The results showed that DBP significantly reduced the anogenital distance, testicular weight, sperm count and motility, and plasma and testicular testosterone levels and significantly increased the oxidative stress, sperm abnormalities, and testicular cell apoptosis...
March 15, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28288491/effect-of-traditional-treatment-of-diabetes-mellitus-with-xanthosoma-sagittifolium-on-the-male-reproductive-system-of-alloxan-induced-diabetic-wistar-rats
#3
Olayinka A Oridupa, Oluyemisi F Folasire, Adedotun J Owolabi, Oluwasanmi Aina
The management of diabetes mellitus (DM) targets glycemic control as well as prevention or reversal of other complications associated with the metabolic disorder. One of such complications is the infertility which has been traced to oxidative stress and DNA damage caused by DM. In this study, experimentally-induced diabetic male Wistar rats were fed with Xanthosoma sagittifolium (cocoyam) for 14 days and its effect on sperm morphology and characteristics, as well as histology of the testes were assessed. X...
March 13, 2017: Drug Research
https://www.readbyqxmd.com/read/28274591/relationships-between-seminal-plasma-metals-metalloids-and-semen-quality-sperm-apoptosis-and-dna-integrity
#4
Yi-Xin Wang, Peng Wang, Wei Feng, Chong Liu, Pan Yang, Ying-Jun Chen, Li Sun, Yang Sun, Jing Yue, Long-Jie Gu, Qiang Zeng, Wen-Qing Lu
This study aimed to investigate the relationships between environmental exposure to metals/metalloids and semen quality, sperm apoptosis and DNA integrity using the metal/metalloids levels in seminal plasma as biomarkers. We determined 18 metals/metalloids in seminal plasma using an inductively coupled plasma-mass spectrometry among 746 men recruited from a reproductive medicine center. Associations of these metals/metalloids with semen quality (n = 746), sperm apoptosis (n = 331) and DNA integrity (n = 404) were evaluated using multivariate linear and logistic regression models...
March 5, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28274316/-x-ray-irradiation-induces-apoptosis-of-mouse-gc1-sperm-cells-via-nuclear-translocation-of-apoptosis-inducing-factor
#5
Huiying Yang, Jingbin Ding, Zhijun Wang, Juan Ding, Xinshe Xia, Wei Zhao
Objective To study the effect of X-ray irradiation on the localization of apoptosis inducing factor (AIF) in mouse GC1 sperm cells. Methods After GC1 cells were treated with 0, 3, 6 and 9 Gy X irradiation, BrdU incorporation assay was performed to detect the proliferation of GC1 cells. Forty-eight hours after irradiation, the nuclear condensation was observed by DAPI staining. The subcellular localization of AIF was showed using the immunofluorescence staining, both in the whole cell extracts and in nuclear extracts, and the expression levels of AIF were detected using Western blot analysis...
March 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28249332/pancreas-transplantation-delays-the-progression-of-morphological-morphometric-and-ultrastructural-changes-in-testes-of-alloxan-induced-diabetic-rats
#6
César Tadeu Spadella, Amélia Arcângela Teixeira Trindade, Amanda Natália Lucchesi, Célia Sperandéo de Macedo
Aim: The aim of this study was to assess the effects of pancreas transplantation on the progression of testicular lesions in diabetic rats. Methods: Diabetic rats were subjected to pancreas transplantation and sacrificed after 6, 14, 26 and 50 weeks of follow-up, using non-diabetic and untreated diabetic rats as controls. Results: Successful pancreas transplantation corrected all of the metabolic changes observed in diabetic rats, including low levels of testosterone. The testicular mass was decreased, and the relative weight of the testes was high in diabetic rats...
February 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28247732/-phosphatidylserine-externalization-and-functional-morphological-impairment-of-sperm-in-men-with-long-barren-marriage
#7
M V Ploskonos
AIM: To identify the relationship between phosphatidylserine externalization, as an early marker of apoptosis, and functional and morphological sperm impairment in infertile men to subsequently evaluate the effect of apoptosis on sperm fertility. MATERIALS AND METHODS: Ejaculates of 18 fertile and 78 subfertile men were examined. Phosphatidylserine externalization was detected by staining the sperm with fluorochrome conjugated Annexin V (AnV-FITC) and propidium iodide using fluorescence microscopy...
August 2016: Urologii︠a︡
https://www.readbyqxmd.com/read/28241054/manchette-acrosome-disorders-during-spermiogenesis-and-low-efficiency-of-seminiferous-tubules-in-hypercholesterolemic-rabbit-model
#8
Layla Simón, Abi K Funes, Martín A Yapur, María E Cabrillana, María A Monclus, Paola V Boarelli, Amanda E Vincenti, Tania E Saez Lancellotti, Miguel W Fornés
Hypercholesterolemia is a marker for several adult chronic diseases. Recently we demonstrated that sub/infertility is also associated to Hypercholesterolemia in rabbits. Seminal alterations included: abnormal sperm morphology, decreased sperm number and declined percentage of motile sperm, among others. In this work, our objective was to evaluate the effects of hypercholesterolemia on testicular efficiency and spermiogenesis, as the latter are directly related to sperm number and morphology respectively. Tubular efficiency was determined by comparing total number of spermatogenic cells with each cell type within the proliferation/differentiation compartments...
2017: PloS One
https://www.readbyqxmd.com/read/28237346/improvement-of-post-thawed-sperm-quality-and-fertility-of-arian-rooster-by-oral-administration-of-d-aspartic-acid
#9
Mahdi Ansari, Mahdi Zhandi, Hamid Kohram, Mojtaba Zaghari, Mostafa Sadeghi, Mohsen Sharafi
This study was conducted to investigate the effect of d-Aspartic acid (D-Asp) on post-thawed sperm quality, fertility and hatchability outcomes in male broiler breeders. Twenty 55-week-old roosters were selected and equally split into four groups (n = 5 rooster/group). Different daily D-Asp doses including 0 (D-0), 100 (D-100), 200 (D-200) or 300 (D-300) mg/kg BW were capsulated and individually administered for 12 weeks to roosters in each group. Semen samples were weekly collected from 7th to 12th week of experiment...
April 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28236448/gradual-decrease-in-spermatogenesis-caused-by-chronic-stress
#10
Lizbeth Juárez-Rojas, Rosa María Vigueras-Villaseñor, Fahiel Casillas, Socorro Retana-Márquez
Chronic stress induces decreased sperm motility, viability and concentration in stressed males. Also, stress modifies oxidative status and causes apoptosis in testes, as well as a decrease in the epithelial area of seminiferous tubules. However, there are no studies that analyze the alterations caused by stress in testicular cells. Thus, in this study, alterations in the morphology of testicular germ cells caused by different days of chronic stress were assessed. Adult male rats were exposed to stress by immersion in cold water (ICW) daily for 3, 8, 20 or 50 consecutive days...
February 21, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28225103/beneficial-effects-of-lycium-barbarum-polysaccharide-on-spermatogenesis-by-improving-antioxidant-activity-and-inhibiting-apoptosis-in-streptozotocin-induced-diabetic-male-mice
#11
Guang-Jiang Shi, Jie Zheng, Jing Wu, Hai-Qi Qiao, Qing Chang, Yang Niu, Tao Sun, Yu-Xiang Li, Jian-Qiang Yu
Spermatogenic dysfunction is one of the major secondary complications of diabetes. L. barbarum polysaccharide (LBP) has long been considered to possess anti-apoptotic activities and antioxidant, anti-inflammatory and fertility-enhancing properties in traditional medical practices in China. The aim of the current study was to seek out scientific evidence to determine if LBP also contributes to the recovery from spermatogenic dysfunction in diabetic individuals. We investigated whether the oral administration of LBP in streptozotocin (STZ)-induced type-1 diabetic male mice would reverse spermatogenic dysfunction and improve histological damage in testes...
February 22, 2017: Food & Function
https://www.readbyqxmd.com/read/28213303/diethylstilbestrol-flutamide-and-their-combination-impaired-the-spermatogenesis-of-male-adult-zebrafish-through-disrupting-hpg-axis-meiosis-and-apoptosis
#12
Pan Yin, Ying-Wen Li, Qi-Liang Chen, Zhi-Hao Liu
Both diethylstilbestrol (DES, an environmental estrogen) and flutamide (FLU, an anti-androgen) are found to impair spermatogenesis by disrupting hypothalamic-pituitary-gonadal (HPG) axis and altering androgen levels through different mechanisms/modes of action in fish with poorly understood underlying mechanisms. Furthermore, it is not known whether and how a combined exposure of DES and FLU has a stronger effect than the compounds alone. In this study, male zebrafish adults were exposed to DES, FLU and their combination (DES+FLU) for 30days, and their effects on histological structure and sperm count in testis, androgen level in plasma, as well as the mRNA levels of genes involved in HPG axis, meiotic regulation and apoptosis were analyzed...
April 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28205391/influence-of-iodine-in-excess-on-seminiferous-tubular-structure-and-epididymal-sperm-character-in-male-rats
#13
Amar K Chandra, Arijit Chakraborty
Excess iodine induced public health problems are now emerging in many iodine sufficient regions for indiscriminate intake of iodine through various iodized products. It has been reported that excess iodine can disrupt overall male reproductive physiology by generating oxidative stress in the testis. However, information on the possible effect of iodine in excess on spermatozoa found less. In the present investigation flow cytometric techniques and scanning electron microscopy (SEM) have been used to study the spermatozoal functional as well as structural status under the influence of excess iodine; generation of ROS in the spermatozoa as evident by DCFDA, altered acrosomal integrity as observed by fluorescence lectin staining method and depolarized mitochondrial membrane potential (ΔΨm ) noticed by JC-1 staining...
February 16, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28182318/molecular-and-structural-changes-related-to-hepatitis-e-virus-hev-antigen-and-its-expression-in-testis-inducing-apoptosis-in-mongolian-gerbil-model
#14
Majid Hussain Soomro, Ruihan Shi, Ruiping She, Yifei Yang, Tongtong Wang, Qiaoxing Wu, Heng Li, Wenzhuo Hao
Hepatitis E virus (HEV) infection has been associated with a wide range of extrahepatic manifestations, so this study was designed to examine the effect and role of HEV on structural and molecular changes in the testicular tissues of Mongolian gerbils experimentally infected with swine HEV. HEV RNA was first detected in testis at 14 day post inoculation and reached a peak between 28 to 42 days later with viral load between 3.12 logs g-¹ to 6.23 logs g-¹ by PCR assays. Microscopic changes showed vacuolation, sloughing of germ cells, formation of multinuclear giant cells, degeneration, necrosis of tubules and damaged blood-testis barrier was observed through transmission electron microscopy...
February 9, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28178639/urinary-metabolites-of-polycyclic-aromatic-hydrocarbons-sperm-dna-damage-and-spermatozoa-apoptosis
#15
Pan Yang, Yi-Xin Wang, Li Sun, Ying-Jun Chen, Chong Liu, Li-Li Huang, Wen-Qing Lu, Qiang Zeng
Inconsistent results between polycyclic aromatic hydrocarbons (PAHs) exposure and adverse male reproductive health have been reported in humans. To assess whether PAH exposure is associated with declined sperm function. Ten monohydroxylated PAHs (OH-PAHs) metabolites were analyzed in repeated urine samples from an infertility clinic. We used multivariable linear models to estimate the associations of urinary OH-PAH metabolites with sperm DNA damage (n=405) and spermatozoa apoptosis (n=366). The shapes of dose-dependent associations of exposure measurements with outcomes were further evaluated by restricted cubic splines...
May 5, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28178360/melatonin-protects-oocyte-quality-from-bisphenol-a-induced-deterioration-in-the-mouse
#16
Mianqun Zhang, Xiaoxin Dai, Yajuan Lu, Yilong Miao, Changyin Zhou, Zhaokang Cui, Honglin Liu, Bo Xiong
Bisphenol A (BPA) has been reported to adversely affect the mammalian reproductive system in both sexes. However, the underlying mechanisms regarding how BPA disrupts the mammalian oocyte quality and how to prevent it have not been fully defined. Here, we document that BPA weakens oocyte quality by impairing both oocyte meiotic maturation and fertilization ability. We find that oral administration of BPA (100 μg/kg body weight per day for 7 days) compromises the first polar body extrusion (78.0% vs 57.0%, P<...
February 8, 2017: Journal of Pineal Research
https://www.readbyqxmd.com/read/28173777/microrna-29c-inhibits-cell-proliferation-by-targeting-nasp-in-human-gastric-cancer
#17
Beiqin Yu, Xuehua Chen, Jianfang Li, Qinlong Gu, Zhenggang Zhu, Chen Li, Liping Su, Bingya Liu
BACKGROUND: Gastric cancer is one of the most common malignancies worldwide. Recent studies have shown that microRNAs play crucial roles in regulating cellular proliferation process in gastric cancer. MicroRNA-29c (miR-29c) acts as a tumor suppressor in different kinds of tumors. METHODS: Quantitative PCR was performed to evaluate miR-29c expression level in 67 patient gastric cancer tissues and 9 gastric cancer cell lines. The effects of miR-29c were explored by proliferation assay, soft agar colony formation assay, apoptosis and cell cycle analysis using flow cytometry...
February 7, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28137755/melatonin-improves-the-fertilization-ability-of-post-ovulatory-aged-mouse-oocytes-by-stabilizing-ovastacin-and-juno-to-promote-sperm-binding-and-fusion
#18
Xiaoxin Dai, Yajuan Lu, Mianqun Zhang, Yilong Miao, Changyin Zhou, Zhaokang Cui, Bo Xiong
STUDY QUESTION: What are the underlying mechanisms of the decline in the fertilization ability of post-ovulatory aged oocytes? SUMMARY ANSWER: Melatonin improves the fertilization ability of post-ovulatory aged oocytes by reducing aging-induced reactive oxygen species (ROS) levels and inhibiting apoptosis and by maintaining the levels and localization of the fertilization proteins, ovastacin and Juno. WHAT IS KNOWN ALREADY: Following ovulation, the quality of mammalian metaphase II oocytes irreversibly deteriorates over time with a concomitant loss of fertilization ability...
January 30, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28137318/thermotolerance-oxidative-stress-apoptosis-heat-shock-proteins-and-damages-to-reproductive-cells-of-insecticide-susceptible-and-resistant-strains-of-the-diamondback-moth-plutella-xylostella
#19
L J Zhang, J L Chen, B L Yang, X G Kong, D Bourguet, G Wu
In this study, we investigated thermotolerance, several physiological responses and damage to reproductive cells in chlorpyrifos-resistant (Rc) and -susceptible (Sm) strains of the diamondback moth, Plutella xylostella subjected to heat stress. The chlorpyrifos resistance of these strains was mediated by a modified acetylcholinesterase encoded by an allele, ace1R, of the ace1 gene. Adults of the Rc strain were less heat resistant than those of the Sm strain; they also had lower levels of enzymatic activity against oxidative damage, higher reactive oxygen species contents, weaker upregulation of two heat shock protein (hsp) genes (hsp69s and hsp20), and stronger upregulation of two apoptotic genes (caspase-7 and -9)...
January 31, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28104681/copy-number-variations-of-e2f1-a-new-genetic-risk-factor-for-testicular-cancer
#20
Maria Santa Rocca, Andrea Di Nisio, Arianna Marchiori, Marco Ghezzi, Giuseppe Opocher, Carlo Foresta, Alberto Ferlin
Testicular germ cell tumor (TGCT) is one of the most heritable forms of cancer. In last years many evidence suggested that constitutional genetic factors, mainly single nucleotide polymorphisms, can increase its risk. However, the possible contribution of copy-number variations (CNVs) in TGCT susceptibility has not been substantially addressed. Indeed, an increasing number of studies have focused on the effect of CNVs on gene expression and on the role of these structural genetic variations as risk factors for different forms of cancer...
January 19, 2017: Endocrine-related Cancer
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