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https://www.readbyqxmd.com/read/29138814/grape-seed-proanthocyanidin-extract-attenuates-varicocele%C3%A2-induced-testicular-oxidative-injury-in-rats-by-activating-the-nrf2%C3%A2-antioxidant-system
#1
Yong Wang, Fan Chen, Ming Liang, Shouzhen Chen, Yaofeng Zhu, Zhichuan Zou, Benkang Shi
The present study investigated whether grape seed proanthocyanidin extract (GSPE) can attenuate varicocele‑induced testicular oxidative injury through the nuclear factor (erythroid‑derived 2)‑like 2 (Nrf2) antioxidant pathway. A varicocele model in rats was established by partial ligation of the left renal vein. Following 4 weeks of GSPE administration, the decreased sperm count and motility and other pathological changes caused by varicocele were significantly alleviated, as indicated by the results of computer‑assisted sperm analysis and hematoxylin and eosin staining...
November 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29119857/mir-638-inhibits-immature-sertoli-cell-growth-by-indirectly-inactivating-pi3k-akt-pathway-via-spag1-gene
#2
Pandi Hu, Kaifeng Guan, Yue Feng, Changping Ma, Huibin Song, Yang Li, Xuanyan Xia, Jialian Li, Fenge Li
Numerous studies have demonstrated that microRNAs (miRNAs) play important roles in cell growth, apoptosis and spermatogenesis. Our previous study showed that miR-638 was differentially expressed in sexually immature and mature testes of Large White boars. Here we reported that sperm-associated antigen 1 (SPAG1) was a direct target gene of miR-638. Moreover, miR-638 inhibited cell proliferation and cell cycle, and promoted apoptosis of porcine immature Sertoli cells. Key genes including phosphorylated phosphatidylinositide 3-kinases (p-PI3K) and phosphorylated serine/ threonine kinase (p-AKT) in PI3K/AKT pathway as well as cell cycle factors including c-MYC, cyclin-D1 (CCND1), cyclin-E1 (CCNE1) and cyclin-dependent kinase 4 (CDK4) were all significantly down-regulated after overexpression of miR-638 or RNAi of SPAG1...
November 9, 2017: Cell Cycle
https://www.readbyqxmd.com/read/29098592/genotoxicity-of-carbon-tetrachloride-and-the-protective-role-of-essential-oil-of-salvia-officinalis-l-in-mice-using-chromosomal-aberration-micronuclei-formation-and-comet-assay
#3
Kawthar Ae Diab, Maha A Fahmy, Zeinab M Hassan, Emad M Hassan, Adel B Salama, Enayat A Omara
The present work was conducted to evaluate the genotoxic effect of carbon tetrachloride (CCl4) in mouse bone marrow and male germ cells. The safety and the modulating activity of sage (Salvia officinalis L.) essential oil (SEO) against the possible genotoxic effect of CCl4 were also evaluated. A combination of in vivo mutagenic endpoints was included: micronucleus (MN), apoptosis using dual acridine orange/ethidium bromide (AO/EB) staining, comet assay, chromosomal aberrations (CAs), and sperm abnormalities...
November 3, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29091894/zinc-alogliptin-combination-attenuates-testicular-toxicity-induced-by-doxorubicin-in-rats-role-of-oxidative-stress-apoptosis-and-tgf-%C3%AE-1-nf-%C3%AE%C2%BAb-signaling
#4
Ahmed M Kabel
The aim of this study was to assess the effect of alogliptin and/or zinc on doxorubicin (DOX)-induced testicular toxicity in rats. Sixty male Wistar rats were divided into 6 equal groups: Control; DOX; DOX+Zinc; DOX+Alogliptin; DOX+Carboxymethyl cellulose and DOX+Zinc+Alogliptin. Testis weight, testicular functions, serum testosterone, luteinizing hormone, follicle stimulating hormone and zinc were measured. Also, testicular tissue zinc, 3 β-hydroxysteroid dehydrogenase, 17 β- hydroxysteroid dehydrogenase, antioxidant enzymes, pro-inflammatory cytokines, transforming growth factor beta 1 (TGF-β1), nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and sperm characteristics were assessed...
October 27, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29069489/effects-of-intraperitoneal-injection-of-sns2-flowers-on-mouse-testicle
#5
Disi Bai, Qingzhao Li, Yanjie Xiong, Junjian Zhao, Liyuan Bai, Peijun Shen, Lu Yuan, Ping Wu
SnS 2 nanoflowers (SnS 2 NFs) have been widely used in photoelectric and catalytic applications. However, its explosure and reproductive toxicity is unknown. The aim of this study was to investigate the effect of exposure to three different sized-SnS 2 flowers (dose: 38 mg/Kg; size: 50, 80 and 200 nm) in testes of mice for 4 weeks by intraperitoneal injection. Though the body weight of mice treated or not with SnS 2 NFs was not different, and SnS 2 NFs were distributed to the organs including liver, kidney, spleen, heart, brain and testis, more distribution SnS 2 NFs (50 and 80 nm) were found in testicle tissues compared with SnS 2 flowers (200 nm) in those tissues...
October 23, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29068181/semen-parameters-in-men-with-varicocele-dna-fragmentation-chromatin-packaging-mitochondrial-membrane-potential-and-apoptosis
#6
Felipe Dieamant, Claudia G Petersen, Ana L Mauri, Vanessa Conmar, Mariana Mattila, Laura D Vagnini, Adriana Renzi, Bruna P Costa, Camila Zamara, João Batista A Oliveira, Ricardo L R Baruffi, José G Franco
OBJECTIVE: To evaluate varicocele effects on conventional semen parameters: sperm DNA fragmentation, chromatin packaging, mitochondrial membrane potential (MMP) and sperm apoptosis. METHODS: A cross-sectional study was carried out with semen samples from 2,399 men of couples who attended an infertility clinic. A total of 16.3% (391/2399) of the men were diagnosed with varicocele by a urologist. RESULTS: A regression analysis revealed that the percentages of sperm with DNA fragmentation, abnormal chromatin packaging, and abnormal MMP were significantly increased in individuals with varicocele, when compared to men without varicocele...
October 25, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29066868/protective-effect-of-da-9401-in-finasteride-induced-apoptosis-in-rat-testis-inositol-requiring-kinase-1-and-c-jun-n-terminal-kinase-pathway
#7
Kiran Kumar Soni, Yu Seob Shin, Bo Ram Choi, Keshab Kumar Karna, Hye Kyung Kim, Sung Won Lee, Chul Young Kim, Jong Kwan Park
Finasteride is used to treat male pattern baldness and benign prostatic hyperplasia. This study investigated the toxicity of finasteride and recovery by DA-9401 using Sprague Dawley (SD) rats. Forty adult male SD rats were assigned to four groups: control (CTR), finasteride 1 mg/kg/day (F), finasteride 1 mg/kg + DA-9401 100 mg/kg/day (F + DA 100) and finasteride 1 mg/kg + DA-9401 200 mg/kg/day (F + DA 200). Treatments were by oral delivery once daily for 90 consecutive days. The gross anatomical parameters assessed included: genital organ weight; vas deferens sperm count and sperm motility; testosterone, dihydrotestosterone (DHT) and malondialdehyde levels; and histological and terminal deoxynucleotidyl transferase enzyme mediated dUTP nick-end labeling (TUNEL) staining of testis for spermatogenic cell density, Johnsen's score and apoptosis...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29053706/solea-senegalensis-sperm-cryopreservation-new-insights-on-sperm-quality
#8
Marta F Riesco, Catarina Oliveira, Florbela Soares, Paulo J Gavaia, María T Dinis, Elsa Cabrita
Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed...
2017: PloS One
https://www.readbyqxmd.com/read/29044638/impact-of-melatonin-supplementation-in-the-rat-spermatogenesis-subjected-to-forced-swimming-exercise
#9
A Moayeri, T Mokhtari, A Hedayatpour, H-A Abbaszadeh, S Mohammadpour, H Ramezanikhah, S Shokri
Oxygen consumption increases many times during exercise, which can increase reactive oxygen species. It negatively affects fertility in male athletes. Melatonin is exerting a regulatory role at different levels of the hypothalamic-pituitary-gonadal axis. However, there is no evidence that the protective effects of melatonin persist after long duration exercise on the spermatogenesis. Therefore, this study was conducted to examine the impacts of melatonin on the testis following the administration of swimming exercise...
October 18, 2017: Andrologia
https://www.readbyqxmd.com/read/29042680/sperm-borne-mir-449b-influences-cleavage-epigenetic-reprogramming-and-apoptosis-of-scnt-embryos-in-bovine
#10
Mengyun Wang, Yang Gao, Pengxiang Qu, Suzhu Qing, Fang Qiao, Yong Zhang, Jesse Mager, Yongsheng Wang
Accumulating evidence indicates the absence of paternally derived miRNAs, piwiRNAs, and proteins may be one important factor contributing to developmental failure in somatic cell cloned embryos. In the present study, we found microRNA-449b (miR-449b) was highly expressed in sperm. Target gene predictions and experimental verification indicate that several embryonic development-related genes, including CDK6, c-MYC, HDAC1 and BCL-2, are targets of miR-449b. We therefore investigated the role of miR-449b using somatic cell nuclear transfer (SCNT) embryo model...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29030185/inhibitory-effect-of-tartrate-against-phosphate-induced-dj-1-aggregation
#11
Min Soo Kim, Sangmin Lee, Sanguk Yun, Pann-Ghill Suh, Jongmi Park, Minghua Cui, Sun Choi, Sun-Shin Cha, Wook Jin
The DJ-1 protein engages in diverse cellular and pathological processes, including tumorigenesis, apoptosis, sperm fertilization, and the progression of Parkinson's disease (PD). The functional dimeric form of DJ-1 transforms into non-functional filamentous aggregates in an inorganic phosphate (Pi)-dependent manner in vitro. Here, we demonstrated that Pi and reactive oxygen species (ROS) induce DJ-1 aggregation in Neuro2A and SH-SY5Y cells. Remarkably, tartrate treatment significantly reduced Pi- and ROS-induced DJ-1 aggregation and restored Pi- and ROS-provoked cell death using quantitative data as mean±standard deviation, and statistics...
October 10, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28992003/rearing-in-captivity-affects-spermatogenesis-and-sperm-quality-in-greater-amberjack-seriola-dumerili-risso-1810
#12
R Zupa, C Fauvel, C C Mylonas, C Pousis, N Santamaria, Μ Papadaki, I Fakriadis, V Cicirelli, S Mangano, L Passantino, G M Lacalandra, Aldo Corriero
The greater amberjack, (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July)...
September 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28985041/influence-of-the-abstinence-period-on-human-sperm-quality-analysis-of-2-458-semen-samples
#13
Vanessa A Comar, Claudia G Petersen, Ana L Mauri, Mariana Mattila, Laura D Vagnini, Adriana Renzi, Bruna Petersen, Andreia Nicoletti, Felipe Dieamant, Joao Batista A Oliveira, Ricardo L R Baruffi, José G Franco
OBJECTIVE: The aim of this study was to evaluate the influence of different periods of abstinence on conventional semen parameters as well as functional parameters in human semen, including mitochondrial function, chromatin packing and sperm DNA fragmentation. METHODS: We recruited a cohort of 2,458 men undergoing infertility investigation. Semen analyses were performed according to WHO guidelines/morphology-motile sperm organelle morphology examination/MSOME. For DNA integrity analysis, the percentages of DNA fragmentation (TUNEL), abnormal chromatin packaging/underprotamination (chromomycin A3/CMA3), abnormal mitochondrial membrane potential (MMP/MitoTracker Green), and apoptosis (annexin-V) were recorded...
October 6, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/28982938/cellular-and-molecular-responses-of-adult-testis-to-changes-in-nutrition-novel-insights-from-the-sheep-model
#14
REVIEW
Yongjuan Guan, Graeme B Martin
This review explores the cellular and molecular mechanisms that regulate spermatogenesis in the post-pubertal testis that is regressing in response to mild undernutrition, using the sexually mature male sheep as a model. Testis regression leads to reductions in daily sperm production and in the quality of ejaculated spermatozoa (poorer movement, DNA damage). There is also a reduction in spermatogenic efficiency that appears to be caused, at least partially, by increases in germ cell apoptosis. Sertoli cell number does not change with testis regression, although about 1% of Sertoli cells do appear to retain proliferative ability after puberty...
November 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28982686/regulation-of-mitosis-meiosis-transition-by-the-ubiquitin-ligase-%C3%AE-trcp-in-male-germ-cells
#15
Tadashi Nakagawa, Teng Zhang, Ryo Kushi, Seiji Nakano, Takahiro Endo, Makiko Nakagawa, Noriko Yanagihara, David Zarkower, Keiko Nakayama
The mitosis-meiosis transition is essential for spermatogenesis. Specific and timely down-regulation of the transcription factor DMRT1, and consequent induction of Stra8 expression, is required for this process in mammals, but the molecular mechanism has remained unclear. Here we show that β-TrCP, the substrate recognition component of an E3 ubiquitin ligase complex, targets DMRT1 for degradation and thereby controls the mitosis-meiosis transition in mouse male germ cells. Conditional inactivation of β-TrCP2 in male germ cells of β-TrCP1 knockout mice resulted in sterility due to a lack of mature sperm...
October 5, 2017: Development
https://www.readbyqxmd.com/read/28973639/1-2-dichloroethane-induces-reproductive-toxicity-mediated-by-the-crem-creb-signaling-pathway-in-male-nih-swiss-mice
#16
Yating Zhang, Guoliang Li, Yizhou Zhong, Manqi Huang, Jiejiao Wu, Jiewei Zheng, Weifeng Rong, Lihai Zeng, Xiao Yin, Fengrong Lu, Zhiwei Xie, Dandan Xu, Qiming Fan, Xiaohui Jia, Ting Wang, Qiansheng Hu, Wen Chen, Qing Wang, Zhenlie Huang
1,2-Dichloroethane (1,2-DCE) is a widely used chlorinated organic toxicant, but little is known about the reproductive disorders induced by its excessive exposure. To reveal 1,2-DCE-induced male reproductive toxicity and to elucidate the underlying mechanisms, we exposed male NIH Swiss mice to 1,2-DCE by inhalation at 0, 100, 350, and 700 mg/m3 for 6 h/day, for 1 and 4 weeks. Our findings showed a significant decrease in body weight with increased testis/body weight ratio, reduced sperm concentration and induced malformation of spermatozoa, and vacuolar degeneration of germ cells in the seminiferous tubules of testes in mice exposed to 1,2-DCE...
August 30, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/28963002/protective-effects-of-apigenin-against-acrylonitrile-induced-subchronic-sperm-injury-in-rats
#17
Yuhui Dang, Zhilan Li, Boyan Luo, Li Pan, Qian Wei, Yinmei Zhang
This study aimed to investigate the protective effects of apigenin (AP), a flavonoid found in plants, against acrylonitrile (ACN)-induced subchronic sperm and testes injury in rats. Male Sprague-Dawley rats were randomly divided into four groups: a control group (corn oil), an ACN group (ACN 50 mg kg(-1)), an ACN + AP1 group (ACN + AP 234 mg kg(-1)), and an ACN + AP2 group (ACN + AP 468 mg kg(-1)). The ACN + AP group received AP by gavage after treatment with 50 mg kg(-1) ACN for 30 min, whereas the rats in the control group were given an equivalent volume of corn oil...
September 28, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28961540/the-reproductive-toxicology-of-male-sd-rats-after-pm2-5-exposure-mediated-by-the-stimulation-of-endoplasmic-reticulum-stress
#18
Xiaona Liu, Xiaoting Jin, Ruijun Su, Zhuoyu Li
Evidence has shown that exposure to fine particulate matter (PM) contributed to poor semen quality in males. However, the reproductive toxicity and relevant molecular mechanisms of Particular Matter 2.5 (PM2.5) from different seasons are not well understood. In the present work, we intend to investigate the toxic effects of PM2.5 during summer and winter on reproductive cells and tissues and focus on endoplasmic reticulum stress (ERS) to illustrate the possible molecular mechanisms. Sprague Dawley (SD) rats were exposed to PM2...
September 20, 2017: Chemosphere
https://www.readbyqxmd.com/read/28956719/arsenic-trioxide-exposure-impairs-testicular-morphology-in-adult-male-mice-and-consequent-fetus-viability
#19
Raquel Frenedoso da Silva, Cibele Dos Santos Borges, Celina de Almeida Lamas, Valéria Helena Alves Cagnon, Wilma de Grava Kempinas
The acute promyelocytic leukemia (APL) is a rare disease, affecting 0.1/100,000 individuals globally. Despite significant advances in APL therapy, some patients still experience relapsed disease. Currently, arsenic trioxide (As2O3) was found to be effective in relapsed APL treatment and considered as standard treatment for these cases. However, it has been shown that exposure to As2O3 may exert adverse effects on the male reproductive system since this substance might also induce apoptosis of other important cell types including stem cells...
2017: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/28947251/supplementation-of-freezing-media-with-stromal-cell-derived-factor-1%C3%AE-preserves-human-sperm-from-cryodamage
#20
Behnaz Hatef, Amirhossein Taromchi, Reza Nejatbakhsh, Ahmad Farrokhi, Saeed Shokri
The destructive effects of sperm cryopreservation result in reduced sperm motility and increased apoptosis. Oocytes, endometrium, and follicular fluid express stromal cell-derived factor-1 alpha (SDF-1α) or C-X-C motif chemokine ligand 12 (CXCL12) while its specific receptor chemokine, CXC motif receptor 4 (CXCR4) is expressed in the head of sperm. SDF-1α can increase sperm motility and preserve normal mitochondrial status. The present study intends to investigate whether the addition of SDF-1α to freezing extender can facilitate cryosurvival of spermatozoa and how SDF-1α protects spermatozoa against damages during cryopreservation...
September 22, 2017: Cryobiology
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