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Sperm apoptosis

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https://www.readbyqxmd.com/read/28795045/evaluation-of-sperm-protamine-deficiency-and-apoptosis-in-infertile-men-with-idiopathic-teratozoospermia
#1
Fatemeh Dehghanpour, Nasim Tabibnejad, Farzaneh Fesahat, Fatemeh Yazdinejad, Ali Reza Talebi
OBJECTIVE: Sperm morphology plays an important role in infertility, especially in cases of defects in the heads of spermatozoa. Tapered-head or elongated-head spermatozoa are examples of morphological abnormalities. The aim of this study was to compare the semen parameters, levels of protamine deficiency, and frequency of apoptosis between patients with normozoospermia and those with teratozoospermia with tapered-head spermatozoa. METHODS: Fifty-two semen samples (27 patients with tapered-head sperm and 25 fertile men) were collected and semen analysis was performed according to the World Health Organization criteria for each sample...
June 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28783592/the-efficiency-of-spermatogenesis-and-the-support-capacity-of-sertoli-cells-in-characiformes
#2
Maira da Silva Rodrigues, Diógenes Henrique de Siqueira-Silva, Patrícia Postingel Quirino, Alexandre Ninhaus-Silveira, Rosicleire Veríssimo-Silveira
This stereological analysis of the types of germ cells and the number of Sertoli cells per cyst in Astyanax altiparanae testes during spermatogenesis is the first such report in Characiformes. Testes of 25 male A. altiparanae were examined. Based on the number of spermatogonia B per cyst (469.2 ± 9.92), we estimated that spermatogonia undergo at least nine mitotic divisions before differentiating into primary spermatocytes. There are four spermatogonia types: undifferentiated spermatogonia A*, undifferentiated spermatogonia, differentiated spermatogonia, and type B spermatogonia...
July 27, 2017: Theriogenology
https://www.readbyqxmd.com/read/28782859/effect-of-supplementation-of-recombinant-regucalcin-in-extender-on-cryopreservation-of-spermatozoa-of-water-buffalo-bubalus-bubalis
#3
Harikrishna Pillai, Mehtab S Parmar, A M Shende, Jobin Thomas, Harikumar S, G Taru Sharma, S K Ghosh, A Kumaresan, S K Bhure
Elevated intracellular calcium concentration and oxidative damage are two major factors contributing to the poor fertility of cryopreserved spermatozoa. Regucalcin (RGN), also known as Senescence marker protein-30 (SMP-30), is a calcium-binding protein with multiple roles that include calcium homeostasis, anti-oxidative, anti-apoptosis, and anti-proliferation. In Drosophila, regucalcin is reportedly a putative cold-tolerance gene, and a cryoprotective role for regucalcin against intracellular calcium elevation and oxidative stress was reported in P19 cell lines...
August 7, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28770381/effects-of-ascorbic-acid-on-spermatogenesis-and-sperm-parameters-in-diabetic-rats
#4
M Verónica Aguirre-Arias, Victoria Velarde, Ricardo D Moreno
Diabetes mellitus in human and animal models has been correlated with low sperm count, testicular abnormalities, high levels of germ cell death, and oxidative stress. In this study, we focus on three questions: (1) Is germ cell apoptosis stage-specific in diabetic male rats? (2) Could ascorbic acid (AA) reverse oxidative and histological damage and restore testicular dysfunction? (3) Could AA treatment restore fertility parameters in diabetic rats? Adult Sprague-Dawley rats were divided into four groups: control, diabetic, control plus AA, and diabetic plus AA...
August 2, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28765938/rapamycin-inhibits-spermatogenesis-by-changing-the-autophagy-status-through-suppressing-mechanistic-target-of-rapamycin-p70s6-kinase-in-male-rats
#5
Shangjing Liu, Longxian Huang, Yanqing Geng, Junlin He, Xuemei Chen, Hao Xu, Rong Li, Yingxiong Wang, Yubin Ding, Xueqing Liu
Rapamycin (sirolimus) is an antiproliferative drug that has been widely used in the clinic as an immunosuppressant and a potential anticancer agent. Certain reports have indicated that rapamycin may induce male infertility through impairing sperm quality. The present study investigated the mechanism of male infertility caused by rapamycin and examined whether withdrawal of rapamycin could recover the number of sperm in rats. Male Sprague‑Dawley rats (n=100) were divided randomly into 5 groups: 3 rapamycin‑treated groups (2, 4 and 6 mg/kg) and 2 control groups [Blank and dimethyl sulfoxide (DMSO)]...
July 31, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28754923/tauroursodeoxycholic-acid-enhances-the-development-of-porcine-embryos-derived-from-in-vitro-matured-oocytes-and-evaporatively-dried-spermatozoa
#6
Xiao-Xia Li, Yun-Fei Diao, Hai-Jun Wei, Shi-Yong Wang, Xin-Yan Cao, Yu-Fei Zhang, Tong Chang, Dan-Li Li, Min Kyu Kim, Baozeng Xu
Evaporative drying (ED) is an alternative technique for long-term preservation of mammalian sperm, which does not require liquid nitrogen or freeze-drying equipment, but offers advantages for storage and shipping at ambient temperature and low cost. However, the development of zygotes generated from these sperms was poor. Here, we demonstrated that the supplementation of tauroursodeoxycholic acid (TUDCA), an endogenous bile acid, during embryo culture improved the developmental competency of embryos derived from in vitro matured pig oocytes injected intracytoplasmically with boar ED spermatozoa by reducing the production of reactive oxygen species, the DNA degradation and fragmentation, and the expression of apoptosis-related gene Bax and Bak, and by increasing the transcription of anti-apoptosis gene Bcl-XL and Bcl-2...
July 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28752572/effect-of-paracetamol-on-semen-quality
#7
REVIEW
S A Banihani
Several published studies, both direct and indirect, have connected paracetamol, also named acetaminophen, a commonly used over-the-counter analgesic and antipyretic medication, with semen quality and male infertility, although as yet this connection is unclear. This review addresses the effect of paracetamol on semen quality and hence on male factor infertility. We searched the MEDLINE database from January 1980 through January 2017 for English-language articles using the key words "paracetamol" and "acetaminophen" versus "sperm...
July 28, 2017: Andrologia
https://www.readbyqxmd.com/read/28750295/effect-of-zearalenone-on-circulating-testosterone-concentration-testicular-and-epididymal-morphology-and-epididymal-sperm-characteristics-in-wild-boars
#8
W Bielas, W Niżański, J Nicpoń, J E Nicpoń, A Partyka, R Mordak, M Nowak, R Ciaputa
This study investigated the effect of exposure to zearalenone (ZEN) and its metabolites on the characteristics of epididymal spermatozoa, testicular and epididymal biometry and histology, and the concentration of testosterone in blood plasma in male wild boars. The study was performed during more than one year on 18 clinically healthy male wild boars with initial and final body weight, of 39 ± 4 kg and 59 ± 3 kg, respectively. The animals were divided into two experimental groups (group I and group II) and one control group (group C) comprising 6 boars per group...
July 18, 2017: Theriogenology
https://www.readbyqxmd.com/read/28744523/evaluation-of-intracellular-anion-superoxide-level-heat-shock-protein-a2-and-protamine-positive-spermatozoa-percentages-in-teratoasthenozoospermia
#9
Parvin Sabeti, Fardin Amidi, Seyed Mahdi Kalantar, Mohammad Ali Sedighi Gilani, Soheila Pourmasumi, Atefeh Najafi, Ali Reza Talebi
BACKGROUND: Teratoasthenozoospermia (TA) is a severe form of male infertility with no clear etiology. OBJECTIVE: To compare the level of intracellular anion superoxide (O2-), heat shock protein A2 (HSPA2) and protamine deficiencies in ejaculated spermatozoa between teratoasthenozoospermic and normozoospermic men. MATERIALS AND METHODS: In this case- control study, semen samples of 20 infertile men, with TA (with normal morphology lower than 4%_ and total motility lower than 40% ) as the case group and 20 normozoospermic fertile men as the control group were evaluated for intracellular O2(-) and HSPA2 by flow cytometry and protamine deficiency by Chromomycin A3 (CMA3) test...
May 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/28744317/podophyllotoxin-extracted-from-juniperus-sabina-fruit-inhibits-rat-sperm-maturation-and-fertility-by-promoting-epididymal-epithelial-cell-apoptosis
#10
Shuwu Xie, Guoting Li, Lijuan Qu, Ruihua Zhong, Ping Chen, Zhigang Lu, Jieyun Zhou, Xiangjie Guo, Zhao Li, Aying Ma, Yueying Qian, Yan Zhu
This study aimed to investigate the antifertility effect of Juniperus sabina fruit on male rats and its possible mechanism, and hence it might be developed as a potential nonhormonal male contraceptive. Male rats were intragastrically fed for consecutive 8-week and 4-week recovery with the fruit of J. Sabina, and sperm maturation, serum testosterone level, and histopathology were analyzed. Epididymal epithelial cell culture was prepared for detection of podophyllotoxin activities. Furthermore, cell proliferation, transmission electron microscopy, Annexin V/Propidium iodide, TUNEL, RT-PCR, ELISA, and western blotting were examined...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28732331/an-evaluation-of-dna-damage-in-human-lymphocytes-and-sperm-exposed-to-methyl-methanesulfonate-involving-the-regulation-pathways-associated-with-apoptosis
#11
Khaled Habas, Mojgan Najafzadeh, Adolf Baumgartner, Martin H Brinkworth, Diana Anderson
Exposure to DNA-damaging agents produces a range of stress-related responses. These change the expression of genes leading to mutations that cause cell cycle arrest, induction of apoptosis and cancer. We have examined the contribution of haploid and diploid DNA damage and genes involved in the regulation of the apoptotic process associated with exposure, The Comet assay was used to detect DNA damage and quantitative RT-PCR analysis (qPCR) to detect gene expression changes in lymphocytes and sperm in response to methyl methanesulfonate...
October 2017: Chemosphere
https://www.readbyqxmd.com/read/28730645/effect-of-metformin-on-germ-cell-specific-apoptosis-oxidative-stress-and-epididymal-sperm-quality-after-testicular-torsion-detorsion-in-rats
#12
M Ghasemnejad-Berenji, M Ghazi-Khansari, I Yazdani, M Nobakht, A Abdollahi, H Ghasemnejad-Berenji, J Mohajer Ansari, S Pashapour, A R Dehpour
The study was designed to evaluate the effects of metformin on apoptosis and epididymal sperm quality in a rat testicular ischaemia/reperfusion (I/R) injury model. A total of 72 male rats were divided into four groups (n = 18 for each group): group 1 (sham-operated group), group 2 (metformin group), group 3 (torsion/detorsion [T/D] + saline) and group 4 (T/D + 300 mg kg(-1) metformin). Testicular torsion was achieved by rotating the right testis 720° in a clockwise direction for 1 hr. Tissue malondialdehyde (MDA) level and caspase-3 activity increased and the activities of catalase, superoxide dismutase and glutathione peroxidase decreased in comparison with sham-operated group 4 hr after detorsion (p < ...
July 21, 2017: Andrologia
https://www.readbyqxmd.com/read/28722192/roles-of-fas-fasl-mediated-apoptosis-and-inhibin-b-in-the-testicular-dysfunction-of-rats-with-left-side-varicocele
#13
J Zhang, P P Jin, M Gong, J H Guo, K Fang, Q T Yi, R J Zhu
We investigated the role of the Fas/FasL signalling pathway and inhibin B expression in rats with an experimentally induced left-side varicocele. Forty-five Sprague Dawley (SD) male rats were randomly divided into three groups in average: control group, sham group and experimental group. The expression of inhibin B in the rat left testis was analysed at the mRNA and protein levels by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting respectively. The expressions of Fas, FasL and caspase-3 in the left testis were measured by RT-PCR and immunofluorescence...
July 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28719015/bisphenol-a-induced-oxidative-stress-and-apoptosis-in-mice-testes-modulation-by-selenium
#14
S Kaur, M Saluja, M P Bansal
Spermatogenesis, a highly coordinated process, is prone to environmental insults which may lead to impaired spermatogenesis or, at worst, infertility. Bisphenol A (BPA) is a well-known global environmental toxicant and a ubiquitous oestrogenic chemical. This study evaluated the role of selenium (0.5 ppm sodium selenite/kg diet) on spermatogenesis after BPA treatment in different groups of male BALB/c mice: control, selenium, BPA and selenium+BPA. Markers of oxidative stress and apoptosis were evaluated in testis after BPA treatment...
July 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28716598/effect-of-lecithin-nanoliposome-or-soybean-lecithin-supplemented-by-pomegranate-extract-on-post-thaw-flow-cytometric-microscopic-and-oxidative-parameters-in-ram-semen
#15
Mahdieh Mehdipour, Hossein Daghigh Kia, Maryam Nazari, Abouzar Najafi
This investigation was carried out to study the effect of soybean lecithin 1.5% (wt/vol) (0, 2.5, 5 and 7.5 mg l(-1) pomegranate extract (PE)) or PE-loaded lecithin nanoliposome (0, 2.5, 5 and 7.5 mg l(-1)) to Tris-based extender. Sperm motility (CASA), viability, membrane integrity (HOS test), abnormalities, mitochondrial activity, apoptosis status, lipid peroxidation, total antioxidant capacity (TAC)) and antioxidant activities (GPX, SOD) were investigated following freeze-thawing. No significant differences (P < 0...
July 14, 2017: Cryobiology
https://www.readbyqxmd.com/read/28705778/maternal-linuron-exposure-alters-testicular-development-in-male-offspring-rats-at-the-whole-genome-level
#16
Jianwei Bai, Hua Han, Feng Wang, Liyu Su, Hongwei Ding, Xiyin Hu, Binli Hu, Hong Li, Wei Zheng, Yan Li
Linuron is a widely used herbicide; its toxicity on the male reproductive system has been recognized. The current study was designed to explore the molecular mechanism underlying linuron-induced reproductive toxicity. Pregnant rats received daily oral gavage of linuron at the dose of 120mg/kg/d from gestation day (GD)12 to GD17. Tissues from male offspring rats were collected for pathological examination and microarray gene expression profiling. Changes in gene expression were further verified by quantitative real-time RT-PCR...
July 11, 2017: Toxicology
https://www.readbyqxmd.com/read/28696579/scrotal-insulation-and-sperm-production-in-the-boar
#17
REVIEW
John J Parrish, Kilby L Willenburg, Katelynn M Gibbs, Kylie B Yagoda, Megan M Krautkramer, Teyanna M Loether, Fabiana C S A Melo
Seasonal infertility is a limiting factor in boar fertility, and is increasingly important as climate changes. Spermatogenesis in the boar produces 256 spermatozoa per type A1 spermatogonium, but the process is inefficient such that only 10-30% of these potential spermatozoa are actually produced. Heat further impacts spermatogenesis by reducing the number of specific germ cells produced while increasing the fraction of abnormal sperm. Early studies used whole-animal exposure to simulate seasonal exposure to heat under production settings, but this approach is associated with many confounding factors that make assessment of the mechanisms of heat-induced damage to spermatogenesis difficult...
July 11, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28694462/transgenerational-inheritance-of-susceptibility-to-diabetes-induced-male-subfertility
#18
Gabriela Pavlinkova, Hasmik Margaryan, Eva Zatecka, Eliska Valaskova, Fatima Elzeinova, Alena Kubatova, Romana Bohuslavova, Jana Peknicova
Male infertility is a worldwide problem associated with genetic background, environmental factors, and diseases. One of the suspected contributing factors to male infertility is diabetes mellitus. We investigated the molecular and morphological changes in sperms and testicular tissue of diabetic males. The study was performed in streptozotocin-induced type 1 diabetes mouse model. Diabetes decreased sperm concentration and viability and increased sperm apoptosis. Changes in protamine 1/protamine 2 ratio indicated reduced sperm quality...
July 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28670429/tumor-necrosis-factor-alpha-308-g-a-single-nucleotide-polymorphism-and-risk-of-sperm-abnormalities-in-iranian-males
#19
Maryam Khademi Bami, Masoud Dehghan Tezerjani, Fateme Montazeri, Hamid Reza Ashrafzadeh Mehrjardi, Saeed Ghasemi-Esmailabad, Mohammad Hasan Sheikhha, Seyed Mehdi Kalantar
BACKGROUND: Signaling molecules such as cytokines regulate spermatogenesis during the maturation of germ cells and sperm apoptosis. Tumor necrosis factor alpha (TNFα) is one of the most-documented cytokines that is involved in spermatogenesis. We investigated the association of the TNFα -308 G/A single nucleotide polymorphism with sperm abnormalities in Iranian males. MATERIALS AND METHODS: This case-control study included 180 infertile men who re- ferred to Yazd Research and Clinical Center for Infertility and 100 healthy normospermic controls...
July 2017: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/28666211/carnitine-organic-cation-transporter-2-octn2-contributes-to-rat-epididymal-epithelial-cell-growth-and-proliferation
#20
Dong Li, Jiumin Liu, Wei Du, Huang Liu, Weilin Xiao, Xiaosong Song, Zhaoying Fan, Chuangbo Ke, Qiangguo Yu, Weibing Qin, Yunge Tang, Xiaoyong Pu
Carnitine/organic cation transporter 2 (OCTN2) is localized at the basolateral membrane of epididymal epithelial cells, and mainly serves to reabsorb carnitine as an essential factor for sperm maturation; however, its functional features in epididymal epithelial cells have remained unclear. We isolated primary epididymal epithelial cells from rat epididymides and verified their phenotype by detecting the presence of cytokeratin-19 (CK-19, an epithelial cell marker) and the absence vimentin (an interstitial cell marker)...
September 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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