keyword
MENU ▼
Read by QxMD icon Read
search

Sperm apoptosis

keyword
https://www.readbyqxmd.com/read/29785804/can-scsa-and-tunel-forecast-apoptosis-related-motility-depletion-in-asthenozoospermia
#1
Z Moradian Fard, M Naghdi, P Salehi, A Ajami, M R Deemeh, M H Meshkibaf
This study is an attempt to determine the power of SCSA and TUNEL for the evaluation of apoptosis status and apoptosis-related motility depletion in Asthenozoospermia. Fifty-one semen samples from Asthenozoospermic and 20 samples from fertile men participated in this study. SCSA and TUNEL were applied for the assessment of DNA integrity by flow cytometry. Annexin V conjugated with FITC labelling and FLICA method were used for the assessment of externalisation of phosphatidylserine and spermatozoon with active Caspase 3 respectively...
May 21, 2018: Andrologia
https://www.readbyqxmd.com/read/29785601/alteration-in-apoptotic-rate-of-testicular-cells-and-sperms-following-administration-of-bisphenol-a-bpa-in-wistar-albino-rats
#2
Seema Srivastava, Priya Gupta
The aim of the study was to evaluate the effect of Bisphenol A [BPA] widely used as a plasticizer in the formation of polycarbonate plastics and epoxy resins, exposure causing alteration in apoptosis rate, and protective effect of Vitamin E when supplemented with BPA orally. Adult male Wistar albino rats aged 3 months were randomly divided into seven groups: control (olive oil treated) BPA-treated (dose 5, 50,100 μg/100gmBW) and Vitamin E intervention group (dose 5, 50, 100 μg/100gmBW BPA+ Vitamin E dose 4 mg/100gmBW)...
May 21, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29774101/qilin-pills-alleviate-oligoasthenospermia-by-inhibiting-bax-caspase-9-apoptosis-pathway-in-the-testes-of-model-rats
#3
Kaishu Zhang, Zhengyan Ge, Longlong Fu, Qi An, Fang Zhou, Ying Guo, Xiaowei Wang, Wenhong Lu, Xiaowei Liang, Shusong Wang, Xuejun Shang, Yiqun Gu
At present, the treatment of oligoasthenospermia with western medicine is ineffective. Qilin pill (QLP) is a Chinese traditional medicine for treating male infertility. Recent multicenter clinical studies in China reported that QLPs markedly improved sperm quality. However, the mechanism of action of QLPs on oligoasthenospermia remains unknown. In this study, we investigated the mechanistic basis for improvement of semen parameters and reversal of testis damage by QLPs in a rat model of oligoasthenospermia induced by treatment with tripterygium glycosides (TGs) (40 mg/kg) once daily for 4 weeks...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29770471/protective-effects-of-the-hydroalcoholic-extract-of-fumaria-parviflora-on-testicular-injury-induced-by-torsion-detorsion-in-adult-rats
#4
M Shokoohi, H Shoorei, M Soltani, S-H Abtahi-Eivari, R Salimnejad, M Moghimian
This study was designed to determine the effects of daily oral administration (250 mg/kg) of the hydroalcoholic extract of Fumaria parviflora (FP) for 14 days on the sperm parameters, oxidative stress parameters, serum testosterone levels, expression of Bax and Bcl-2 genes, and apoptosis index of germ cells after testicular torsion-detorsion (ischaemia-reperfusion, IR) injury model in rats. Twenty-eight adult male Wistar rats were divided randomly into four groups of seven each: sham operation, torsion-detorsion (TD), TD plus the hydroalcoholic extract FP (TDFP) and only FP without TD application (FP)...
May 16, 2018: Andrologia
https://www.readbyqxmd.com/read/29769570/in-utero-and-postnatal-exposure-to-high-fat-high-sucrose-diet-suppressed-testis-apoptosis-and-reduced-sperm-count
#5
Jiude Mao, Kathleen A Pennington, Omonseigho O Talton, Laura C Schulz, Miriam Sutovsky, Yan Lin, Peter Sutovsky
Obesity affects male fertility and maternal diabetes affects the offspring sperm epigenome. However, the effects of in utero exposure to maternal glucose intolerance in combination with postnatal high fat, high sucrose (HFHS) diet consumption on offspring spermatogenesis is not clear. The present study was designed to test these effects. One week before and during pregnancy, dams were fed either control or HFHS diet to induce gestational glucose intolerance, and returned to standard diet during lactation. Male offspring from each maternal group were split into control and HFHS-fed groups for eight weeks prior to sacrifice at 11, 19 or 31 weeks of age, and reproductive tissues were harvested for analysis of testicular germ cell apoptosis and sperm output...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769123/obesity-impairs-male-fertility-through-long-term-effects-on-spermatogenesis
#6
Yan-Fei Jia, Qian Feng, Zheng-Yan Ge, Ying Guo, Fang Zhou, Kai-Shu Zhang, Xiao-Wei Wang, Wen-Hong Lu, Xiao-Wei Liang, Yi-Qun Gu
OBJECTIVE: This study aimed to investigate the effect and possible underlying mechanisms of high-fat diet-induced obesity on spermatogenesis in male rats. METHODS: A total of 45 male rats were randomly divided into control (n = 15, normal diet) and obesity groups (n = 30, high-fat diet) and were fed for 16 weeks. Body weight and organ indexes were determined after sacrifice. Indicators of reproductive function, including sperm count, sperm motility, apoptosis of spermatogenic cells, and oxidative stress levels, were measured...
May 16, 2018: BMC Urology
https://www.readbyqxmd.com/read/29765499/effect-of-heat-stress-on-sperm-dna-protamine-assessment-in-ram-spermatozoa-and-testicle
#7
Thais Rose Dos Santos Hamilton, Adriano Felipe Perez Siqueira, Letícia Signori de Castro, Camilla Mota Mendes, Juliana de Carvalho Delgado, Patrícia Monken de Assis, Leonardo Pereira Mesquita, Paulo César Maiorka, Marcílio Nichi, Marcelo Demarchi Goissis, José Antonio Visintin, Mayra Elena Ortiz D Ávila Assumpção
Sperm DNA fragmentation is considered one of the main causes of male infertility. The most accepted causes of sperm DNA damage are deleterious actions of reactive oxygen species (ROS), defects in protamination, and apoptosis. Ram sperm are highly prone to those damages due to the high susceptibility to ROS and to oxidative stress caused by heat stress. We aimed to evaluate the effects of heat stress on the chromatin of ejaculated and epididymal sperm and the activation of apoptotic pathways in different cell types in ram testis...
2018: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29765076/resveratrol-significantly-improves-the-fertilisation-capacity-of-bovine-sex-sorted-semen-by-inhibiting-apoptosis-and-lipid-peroxidation
#8
Chong-Yang Li, Ya-Han Zhao, Hai-Sheng Hao, Hao-Yu Wang, Jin-Ming Huang, Chang-Liang Yan, Wei-Hua Du, Yun-Wei Pang, Pei-Pei Zhang, Yan Liu, Hua-Bin Zhu, Xue-Ming Zhao
The aim of this study was to test the effects of five different concentrations (0, 10-3 , 10-4 , 10-5 , and 10-6  M) of resveratrol (Res) supplementation in bull sperm washing and fertilisation medium on levels of reactive oxygen species (ROS), phosphatidylserine (PS) externalisation, mitochondrial membrane potential (Δψm), ATP and malondialdehyde (MDA), acrosomal integrity, blastocyst rate, and blastocyst quality after in vitro fertilisation (IVF). The results for sex-sorted sperm from three bulls showed: (1) ROS and MDA levels in 10-3  M and 10-4  M Res groups were significantly lower than those of controls (P < 0...
May 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29761655/fennel-induces-cytotoxic-effects-against-testicular-germ-cells-in-mice-evidences-for-suppressed-pre-implantation-embryo-development
#9
Aram Minas, Gholamreza Najafi, Ali Shalizar Jalali, Mazdak Razi
Foeniculum vulgare (FVE; fennel) is an aromatic plant belonging to Umbelliferae family, which is widely used in traditional societies because of its different pharmaceutical properties. To uncover the fennel-derived essential oil (FVEO)-induced effects on male reproductive potential, 24 mature male albino mice were divided into, control, 0.37, 0.75, and 1.5 mg kg-1 FVEO-received groups. Following 35 days, the animals were euthanized and the testicular tissue and sperm samples were collected. The histological alterations, tubular differentiation (TDI), spermiogenesis (SPI) indices, apoptosis ratio, and RNA damage of germinal cells were analyzed...
May 15, 2018: Environmental Toxicology
https://www.readbyqxmd.com/read/29759112/remodelling-of-mitochondria-during-spermiogenesis-of-chinese-soft-shelled-turtle-pelodiscus-sinensis
#10
Abdul Haseeb, Hong Chen, Yufei Huang, Ping Yang, Xuejing Sun, Adeela Iqbal, Nisar Ahmed, Taozhi Wang, Noor Samad Gandahi, Xuebing Bai, Qiusheng Chen
Mitochondria are vital cellular organelles that have the ability to change their shape under different conditions, such as in response to stress, disease, changes in metabolic rate, energy requirements and apoptosis. In the present study, we observed remodelling of mitochondria during spermiogenesis and its relationship with mitochondria-associated granules (MAG). At the beginning of spermiogenesis, mitochondria are characterised by their round shape. As spermiogenesis progresses, the round-shaped mitochondria change into elongated and then swollen mitochondria, subsequently forming a crescent-like shape and finally developing into onion-like shaped mitochondria...
May 15, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29749435/spag6-silencing-induces-apoptosis-in-the-myelodysplastic-syndrome-cell-line-skm%C3%A2-1-via-the-pten-pi3k-akt-signaling-pathway-in-vitro-and-in-vivo
#11
Jiaxiu Yin, Xinxin Li, Zaili Zhang, Xiaohua Luo, Li Wang, Lin Liu
Apoptosis is a multi-step mechanism of cell self‑destruction for maintaining cellular homeostatic balance. Accumulating evidence indicates that abnormal apoptosis promotes the evolution and progression of myelodysplastic syndromes (MDS). As a novel cancer-testis antigen, sperm‑associated antigen 6 (SPAG6) has been reported to regulate apoptosis through the tumor necrosis factor-related apoptosis-inducing ligand signaling pathway in the MDS cell line SKM‑1. However, the mechanism of the intrinsic cell death pathway for apoptosis induction by SPAG6 silencing is unclear...
May 2, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29748930/positive-pcna-and-ki-67-expression-in-the-testis-correlates-with-spermatogenesis-dysfunction-in-fluoride-treated-rats
#12
Wen-Peng Zhao, Hong-Wei Wang, Jing Liu, Pan-Pan Tan, Xiang-Long Luo, Shi-Quan Zhu, Xue-Li Chen, Bian-Hua Zhou
The present study aimed to evaluate the effect of fluoride (F) on spermatogenesis in male rats. F- at 50 and 100 mg/L was administered for 70 days, after which the testicular and epididymis tissues were collected to observe the histopathological structure under a light microscope. The ultrastructure of the testis and sperm was also examined via transmission electron microscopy. The apoptosis of spermatogenic cells was measured through terminal deoxynucleotidyl transferase dUTP nick end labeling staining. The expression of proliferation factors, namely, proliferating cell nuclear antigen (PCNA) and Ki-67, in the testicular and epididymis tissues, were assayed through immunohistochemistry...
May 10, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29748247/reduced-levels-of-stromal-sex-hormone-binding-globulin-and-androgen-receptor-dysfunction-in-the-sperm-storage-region-of-the-rat-epididymis
#13
Fabiane de Santi, Flávia L Beltrame, Barry T Hinton, Paulo S Cerri, Estela Sasso-Cerri
The cauda epididymidis is the major sperm storage region whose androgenic supply, essential for the sperm viability, is provided by the vasculature and is dependent upon testosterone diffusion through the stromal tissue to reach the epithelial cells. We have focused our efforts on examining the regulation of this important epididymal region by evaluating the impact of the androgen disrupter cimetidine on the epithelial-stromal androgenic microenvironment. Male rats received 100 mg/kg cimetidine (CMTG) or saline (CG) for 50 days, serum testosterone levels were measured and the epididymal cauda region was processed for light and transmission electron microscopy...
June 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29745297/sperm-associated-antigen-9-spag9-a-promising-therapeutic-target-of-ovarian-carcinoma
#14
Nirmala Jagadish, Rukhsar Fatima, Aditi Sharma, Sonika Devi, Vitusha Suri, Vikash Kumar, Anil Suri
SPAG9 is a novel tumor associated antigen, expressed in variety of malignancies. However, its role in ovarian cancer remains unexplored. SPAG9 expression was validated in ovarian cancer cells by real time PCR and Western blot. SPAG9 involvement in cell cycle, DNA damage, apoptosis, paclitaxel sensitivity and epithelial- mesenchymal transition (EMT) was investigated employing RNA interference approach. Combinatorial effect of SPAG9 ablation and paclitaxel treatment was evaluated in in vitro. Quantitative PCR and Western blot analysis revealed SPAG9 expression in A10, SKOV-3 and Caov3 compared to normal ovarian epithelial cells...
May 2018: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/29741722/prenatal-exposure-to-bisphenol-a-analogues-on-male-reproductive-functions-in-mice
#15
Mingxin Shi, Nikola Sekulovski, James A MacLean, Kanako Hayashi
This study was performed to examine whether prenatal exposure to bisphenol (BP) A analogues, BPE and BPS, negatively impacts male reproductive functions and testis development using mice as a model. CD-1 mice were orally exposed to control treatment (corn oil), BPA, BPE, and BPS (0.5, 20, or 50 µg/kg/day) from gestational day 11 (the presence of vaginal plug = 1) to birth. Although sperm counts were significantly reduced by BPA, BPE, or BPS on postnatal day (PND) 60, only BPE or BPS exposure remained low for sperm motility...
March 21, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29734414/dog-sperm-cryopreservation-in-glucose-fructose-or-sucrose-supplemented-glycerol-free-tris-effect-of-post-thaw-incubation-time-on-gene-expression-related-to-apoptosis-and-motility
#16
M A Rahman, S H Park, I J Yu
  OBJECTIVE: The study evaluated the effects of glucose-fructose or sucrose supplementation in glycerol-free Tris (GFT) solution on motility, viability, the level of reactive oxygen species (ROS), as well as the level of apoptosis (BAX and BCL2) and motility (SMCP)-related gene expression of dog spermatozoa. MATERIALS AND METHODS: Spermatozoa (5×107 sperm/ml) were cryopreserved in GFT containing 86 mM glucose and 86 mM fructose (GF-GFT) or 100 mM sucrose (S-GFT). Progressive motility, viability, ROS (H2 O2 ) level and mRNA gene expression of spermatozoa were evaluated 0 h, 3 h or 6 h post-thaw at 24°C...
January 2018: Cryo Letters
https://www.readbyqxmd.com/read/29734404/effect-of-tert-butyl-hydroquinone-on-bull-semen-cryopreservation
#17
S M H Hussaini, M Zhandi, A Z Shahneh, M Sharafi, A Nejati-Javaremi, A Yousefi, M Emamverdi, M A M M Shehab-El-Deen, F M Pastor
BACKGROUND: Many studies have been shown that freezing induced oxidative stress has detrimental effect on post-thaw sperm quality. OBJECTIVE: This study was conducted to investigate the effect of tert-butyl hydroquinone (tBHQ) on bull semen crtopreservation. MATERIALS AND METHODS: In this study, four different levels of tBHQ [Optidyl containing zero (T0), 2.5 (T2.5), 5 (T5), and 7.5 µM (T7.5) tBHQ] was used to study the effect of tBHQ on freezability of bull semen...
September 2017: Cryo Letters
https://www.readbyqxmd.com/read/29733323/computational-analysis-of-the-caenorhabditis-elegans-germline-to-study-the-distribution-of-nuclei-proteins-and-the-cytoskeleton
#18
Sandeep Gopal, Roger Pocock
The Caenorhabditis elegans (C. elegans) germline is used to study several biologically important processes including stem cell development, apoptosis, and chromosome dynamics. While the germline is an excellent model, the analysis is often two dimensional due to the time and labor required for three-dimensional analysis. Major readouts in such studies are the number/position of nuclei and protein distribution within the germline. Here, we present a method to perform automated analysis of the germline using confocal microscopy and computational approaches to determine the number and position of nuclei in each region of the germline...
April 19, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29721205/treosulfan-induces-distinctive-gonadal-toxicity-compared-with-busulfan
#19
Mattan Levi, Salomon M Stemmer, Jerry Stein, Ruth Shalgi, Irit Ben-Aharon
Treosulfan (L-treitol-1,4-bis-methanesulfonate) has been increasingly incorporated as a main conditioning protocol for hematopoietic stem cell transplantation in pediatric malignant and non-malignant diseases. Treosulfan presents lower toxicity profile than other conventional alkylating agents containing myeloablative and immunosuppressive traits such as busulfan. Yet, whereas busulfan is considered highly gonadotoxic, the gonadal toxicity profile of treosulfan remains to be elucidated. To study the gonadotoxicity of treosulfan, pubertal and prepubertal male and female mice were injected with treosulfan or busulfan and sacrificed one week, one month or six months later...
April 10, 2018: Oncotarget
https://www.readbyqxmd.com/read/29712649/tolerance-to-paternal-genotoxic-damage-promotes-survival-during-embryo-development-in-zebrafish-danio-rerio
#20
Cristina Fernández-Díez, Silvia González-Rojo, Marta Lombó, M Paz Herráez
Spermatozoa carry DNA damage that must be repaired by the oocyte machinery upon fertilization. Different strategies could be adopted by different vertebrates to face the paternal genotoxic damage. Mammals have strong sperm selection mechanisms and activate a zygotic DNA damage response (DDR) (including cell cycle arrest, DNA repair and alternative apoptosis) in order to guarantee the genomic conformity of the reduced progeny. However, external fertilizers, with different reproductive strategies, seem to proceed distinctively...
April 30, 2018: Biology Open
keyword
keyword
104261
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"