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https://www.readbyqxmd.com/read/28627666/expression-pattern-of-zinc-finger-protein-185-in-mouse-testis-and-its-role-in-regulation-of-testosterone-secretion
#1
Xinguo You, Lu Wei, Shutong Fan, Weiwei Yang, Xiaoying Liu, Guohui Wang, Yi Man, Zhifang Pan, Weiguo Feng
Zinc finger protein 185 (ZNF185) belongs to the ZNF family and is involved in cell proliferation and differentiation. To the best of our knowledge, the association between ZNF185 and male reproduction is unknown. In the present study, the expression and localization of ZNF185 in mouse testis, as well as its role in testosterone secretion, cell cycle progression and apoptosis of mouse Leydig cells were investigated. The results of the immunofluorescence analysis indicated that ZNF185 was highly expressed in Leydig cells of the mouse testis, and primarily localized in the cytoplasm...
June 16, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28627439/mitochondrial-prohibitin-and-its-ubiquitination-during-spermatogenesis-of-the-swimming-crab-charybdis-japonica
#2
Ya-Ru Xu, Yun-Shu Fan, Wan-Xi Yang
It has been proposed that prohibitin (PHB) involved in multiple cellular functions, including cell proliferation, differentiation, apoptosis, senescence and carcinogenesis. Various cellular compartment location of PHB demonstrates its diverse roles. Based on the full-length sequence of PHB gene, we analyzed the deduced amino acid sequence and the predicted protein structure of this gene in the swimming crab Charybdis japonica. It shows that the structure and function of PHB are conservative. The expression level of PHB mRNA and protein in different tissues were analyzed by sqRT-PCR and western blot respectively, which showed its high expression in testis...
June 13, 2017: Gene
https://www.readbyqxmd.com/read/28623529/ameliorative-effect-of-omega-3-on-spermatogenesis-testicular-antioxidant-status-and-preimplantation-embryo-development-in-streptozotocin-induced-diabetes-in-rats
#3
Mehdi Khavarimehr, Vahid Nejati, Mazdak Razi, Gholamreza Najafi
PURPOSE: The present study was done to determine the ameliorative effect of polyunsaturated fatty acid omega-3 against experimental diabetes-induced damages on testicular tissue, sperm parameters and preimplantation embryo development in rat model. METHODS: Thirty-two mature male rats were divided into two control and test groups. The experimental diabetes (50 mg kg(-1) streptozotocin, ip) was induced in test group and subdivided into non-treated diabetic, 300 and 600 mg kg(-1) omega-3-treated (orally by gavage) groups...
June 16, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/28613007/transcriptional-coactivator-with-pdz-binding-motif-is-required-to-sustain-testicular-function-on-aging
#4
Mi Gyeong Jeong, Hyuna Song, Ji Hyun Shin, Hana Jeong, Hyo Kyeong Kim, Eun Sook Hwang
Transcriptional coactivator with PDZ-binding motif (TAZ) directly interacts with transcription factors and regulates their transcriptional activity. Extensive functional studies have shown that TAZ plays critical regulatory roles in stem cell proliferation, differentiation, and survival and also modulates the development of organs such as the lung, kidney, heart, and bone. Despite the importance of TAZ in stem cell maintenance, TAZ function has not yet been evaluated in spermatogenic stem cells of the male reproductive system...
June 14, 2017: Aging Cell
https://www.readbyqxmd.com/read/28601154/spermatozoal-transcripts-expression-levels-are-predictive-of-semen-quality-and-conception-rate-in-bulls-bos-taurus
#5
Sivashanmugam Parthipan, Sellappan Selvaraju, Lakshminarayana Somashekar, Arunachalam Arangasamy, Muniandy Sivaram, Janivara Parameswaraiah Ravindra
Spermatozoal transcripts expression levels could be used to assess fertility potential of a male. The objective of the present study was to elucidate the predictive ability of the expression levels of growth, apoptosis and homeostasis regulating transcripts on sperm functions and fertility. The expression levels of spermatozoal RNA isolated from the neat semen samples were related to the good (discarded ejaculate, <25%; n = 7) and poor (discarded ejaculate, >40%, n = 6) quality semen producer and bulls (n = 12) with known conception rate...
August 2017: Theriogenology
https://www.readbyqxmd.com/read/28592902/ggnbp2-is-necessary-for-testis-morphology-and-sperm-development
#6
Anqi Chen, Jixi Li, Lesheng Song, Chaoneng Ji, Marion Böing, Jinzhong Chen, Beate Brand-Saberi
Gametogenetin Binding Protein 2 (GGNBP2) was identified as a tumor suppressor and verified as such by several studies. GGNBP2 has also been reported to be essential for pregnancy maintenance via regulation of trophoblast stem cells. Gametogenetin (GGN) is a testicular germ cell-specific gene expressed in adult testes. As a potential GGN1-interacting protein, the role of GGNBP2 in spermatogenesis has not yet been clarified. We generated heterozygous GGNBP2 knockout mice and bred them by intercrossing. We found that among the offspring, homozygous GGNBP2 knockout (KO) mice were present in severely reduced numbers...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28580449/administration-of-high-dose-of-methamphetamine-has-detrimental-effects-on-sperm-parameters-and-dna-integrity-in-mice
#7
Mojdeh Sabour, Arezoo Khoradmehr, Seyyed Mehdi Kalantar, Amir Hossein Danafar, Marjan Omidi, Iman Halvaei, Ali Nabi, Saeed Ghasemi-Esmailabad, Ali Reza Talebi
BACKGROUND: Methamphetamine (MA) was shown to have harmful effects on male reproductive system. OBJECTIVE: To investigate probable effects of daily administration of MA on sperm parameters and chromatin/DNA integrity in mouse. MATERIAL AND METHODS: Thirty-five NMRI male mice were divided into five groups including low, medium, and high dosage groups which were injected intraperitoneally with 4, 8 and 15 mg/kg/day for 35 days, respectively...
March 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/28559132/effect-of-reduced-glutathione-supplementation-in-semen-extender-on-tyrosine-phosphorylation-and-apoptosis-like-changes-in-frozen-thawed-hariana-bull-spermatozoa
#8
Nadeem Shah, Vijay Singh, Hanuman Prasad Yadav, Meena Verma, Dharmendra Singh Chauhan, Atul Saxena, Sarvajeet Yadav, Dilip Kumar Swain
To provide new insights into the mechanisms through which reduced glutathione (GSH) is able to protect spermatozoa, we tested the hypothesis that cryocapacitation and apoptosis like changes can contribute to the negative effect of freezing and thawing on bull spermatozoa, and that GSH prevent this damage. Having known protective effects of GSH in terms of a potent antioxidant, we evaluated capacitation, tyrosine phosphorylation and apoptosis like changes in bull spermatozoa after freezing and thawing in egg yolk tris glycerol extender containing (0...
May 20, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28557348/the-toxic-mode-of-action-of-cyclic-lipodepsipeptide-fusaricidins-produced-by-paenibacillus-polymyxa-toward-mammalian-cells
#9
Raimo Mikkola, Maria A Andersson, Pavel Grigoriev, Mari Heinonen, Mirja S Salkinoja-Salonen
AIMS: The toxic strains of Paenibacillus polymyxa were isolated from buildings connected to ill health symptoms. Our aim was to identify the toxic compounds of Paenibacillus polymyxa and describe their toxic actions. METHODS AND RESULTS: The toxins of Paenibacillus polymyxa were purified and analysed by HPLC and mass spectrometry. Toxic fusaricidins A and B, and LI-F05a with mass ions at m/z 883.7, 897.6, and 897.6, respectively, were found. Cytotoxicity of purified fusaricidins A and B were measured using boar sperm, porcine tubular kidney epithelial and murine fibroblast cells...
May 29, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28523277/the-effect-of-l-carnitine-hypotaurine-and-taurine-supplementation-on-the-quality-of-cryopreserved-chicken-semen
#10
Agnieszka Partyka, Olga Rodak, Joanna Bajzert, Joanna Kochan, Wojciech Niżański
The objective of this study was to investigate the effect of L-carnitine (LC), hypotaurine (HT), and taurine (T) on the quality of frozen-thawed chicken semen. Pooled semen samples were divided into seven aliquots (control, 1 mM LC, 5 mM LC, 1 mM HT, 10 mM HT, 1 mM T, and 10 mM T) and subjected to cryopreservation. Postthaw sperm motility was determined by IVOS system and sperm characteristics were assessed with fluorochromes and flow cytometry. The highest sperm motility and the highest percentage of viable sperm were in the HT1 group (P < 0...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28518149/rnf138-deficiency-promotes-apoptosis-of-spermatogonia-in-juvenile-male-mice
#11
Longchang Xu, Yalan Lu, Deqiang Han, Rongyan Yao, Han Wang, Shunshun Zhong, Yanyun Luo, Ruiqin Han, Kai Li, Jun Fu, Shudong Zong, Shiying Miao, Wei Song, Linfang Wang
Spermatogenesis, the process by which haploid sperm cells are produced from a diploid precursor cell, is essential for sexual reproduction. Here, we report that RING-finger protein 138 (Rnf138) is highly expressed in testes, especially in spermatogonia and spermatocytes. The role of Rnf138 in spermatogenesis was examined using a Rnf138-knockout mouse model. Rnf138 deficiency resulted in increased apoptosis in spermatogenic cells, loss of proliferative spermatogonia, delayed development of spermatozoa and impaired fertility...
May 18, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28517043/sperm-chromatin-quality-and-dna-integrity-in-partial-versus-total-globozoospermia
#12
A R Talebi, J Ghasemzadeh, M A Khalili, I Halvaei, F Fesahat
Globozoospermia is a severe form of teratozoospermia with low incidence in infertile patients, considered as one of the important causes of male infertility. The objective was to investigate the chromatin/DNA integrity as well as apoptosis in ejaculated spermatozoa of cases with partial or total globozoospermia. Fifty-seven semen samples were divided into three groups of partial globozoospermia (n = 17), total globozoospermia (n = 10) and normozoospermia (control; n = 30). Sperm chromatin condensation, DNA integrity and apoptosis were assessed using cytochemical assays...
May 18, 2017: Andrologia
https://www.readbyqxmd.com/read/28501977/characterization-of-mitochondrial-prohibitin-from-boleophthalmus-pectinirostris-and-evaluation-of-its-possible-role-in-spermatogenesis
#13
Di Wang, Yong-Qiang Zhao, Ying-Li Han, Cong-Cong Hou, Jun-Quan Zhu
Prohibitin (PHB) is an evolutionarily conserved mitochondrial membrane protein. It plays a vital role in cell proteolysis, senescence, and apoptosis and is associated with spermatogenesis and sperm quality control in mammals. To study the characteristics of the PHB gene and its potential roles during spermatogenesis in Boleophthalmus pectinirostris, we cloned a 1153-bp full-length cDNA from the testis of B. pectinirostris with an open reading frame of 816 bp, which encodes 272 amino acid residues. Real-time quantitative PCR (qPCR) analysis revealed the presence of phb mRNA in all the tissues examined, with higher expression levels found in the testis, kidney, intestine, and muscle tissues...
May 13, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28499169/two-novel-dinuclear-ellipsoid-ni-ii-and-co-ii-complexes-bridged-by-4-5-bis-pyrazol-1-yl-phthalic-acid-synthesis-structural-characterization-and-biological-evaluation
#14
Enjun Gao, Zhenzhen Qi, Yun Qu, Yuqing Ding, Yang Zhan, Na Sun, Shaozhong Zhang, Xue Qiu, Mingchang Zhu
Two novel complexes, [Ni2(L)2(H2O)3]·4(H2O) (1) and [Co2(L)2(H2O)3]·5(H2O) (2) [H2L = 4,5-bis(pyrazol-1-yl) phthalic acid] were synthesized and characterized by spectroscopy (IR, (1)H NMR), and elemental analysis. The structures for the complexes were determined by X-ray crystallography providing the dinuclear ellipsoid Ni(II) and Co(II) complexes bridged by 4,5-bis(pyrazol-1-yl)phthalic acid ligands with same coordination modes. The interaction capacity of the complexes with FS-DNA (fish sperm DNA) has been investigated by UV and fluorescence spectroscopy...
May 4, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28498828/kdm1a-lsd1-regulates-the-differentiation-and-maintenance-of-spermatogonia-in-mice
#15
Dexter A Myrick, Michael A Christopher, Alyssa M Scott, Ashley K Simon, Paul G Donlin-Asp, William G Kelly, David J Katz
The proper regulation of spermatogenesis is crucial to ensure the continued production of sperm and fertility. Here, we investigated the function of the H3K4me2 demethylase KDM1A/LSD1 during spermatogenesis in developing and adult mice. Conditional deletion of Kdm1a in the testis just prior to birth leads to fewer spermatogonia and germ cell loss before 3 weeks of age. These results demonstrate that KDM1A is required for spermatogonial differentiation, as well as germ cell survival, in the developing testis...
2017: PloS One
https://www.readbyqxmd.com/read/28443181/development-of-a-test-method-for-the-evaluation-of-dna-damage-in-mouse-spermatogonial-stem-cells
#16
Hye Lyun Jeon, Jung-Sun Yi, Tae Sung Kim, Youkyung Oh, Hye Jeong Lee, Minseong Lee, Jin Seok Bang, Kinarm Ko, Il Young Ahn, Kyungyuk Ko, Joohwan Kim, Hye-Kyung Park, Jong Kwon Lee, Soo Jung Sohn
Although alternative test methods based on the 3Rs (Replacement, Reduction, Refinement) are being developed to replace animal testing in reproductive and developmental toxicology, they are still in an early stage. Consequently, we aimed to develop alternative test methods in male animals using mouse spermatogonial stem cells (mSSCs). Here, we modified the OECD TG 489 and optimized the in vitro comet assay in our previous study. This study aimed to verify the validity of in vitro tests involving mSSCs by comparing their results with those of in vivo tests using C57BL/6 mice by gavage...
April 2017: Toxicological Research
https://www.readbyqxmd.com/read/28442719/chlamydia-trachomatis-neither-exerts-deleterious-effects-on-spermatozoa-nor-impairs-male-fertility
#17
Jenniffer Puerta Suarez, Leonardo R Sanchez, Florencia C Salazar, Hector A Saka, Rosa Molina, Andrea Tissera, Virginia E Rivero, Walter D Cardona Maya, Ruben D Motrich
Chlamydia trachomatis is the most prevalent sexually transmitted bacterial infection. However, whether Chlamydia trachomatis has a negative impact on sperm quality and male fertility is still controversial. Herein, we report the effects on sperm quality of the in vitro exposure of spermatozoa to Chlamydia trachomatis, and also the effects of male genital infection on male fertility using an animal model. Human and mouse sperm were obtained from healthy donors and cauda epididimys from C57BL/6 mice, respectively...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28430968/sex-chromosome-dependent-differential-viability-of-human-spermatozoa-during-prolonged-incubation
#18
Young-Ah You, Woo-Sung Kwon, Md Saidur Rahman, Yoo-Jin Park, Young-Ju Kim, Myung-Geol Pang
STUDY QUESTION: Are there significant differences in the ability of X chromosome-bearing (X) spermatozoa and Y chromosome-bearing (Y) spermatozoa to survive incubation under stressful conditions? SUMMARY ANSWER: Y spermatozoa are more vulnerable to stress than their X counterparts depending on culture period and temperature, and show higher expression of apoptotic proteins. WHAT IS KNOWN ALREADY: The primary sex ratio is determined by there being an equal number of spermatozoa carrying X and Y chromosomes...
June 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28420799/neonatal-hypothyroidism-does-not-increase-sertoli-cell-proliferation-in-inos-mice
#19
Nathalia de Lima E Martins Lara, Luiz Renato de França
Sertoli cell (SC) proliferation in mice occurs until two weeks after birth and is mainly regulated by FSH and thyroid hormones. Previous studies have shown that transient neonatal hypothyroidism in laboratory rodents is able to extend SC mitotic activity, leading ultimately to higher testis size and daily sperm production (DSP) in adult animals. Moreover, we have shown that due to higher SC proliferation and lower germ cell apoptosis, iNOS deficiency in mice also results in higher testis size and DSP. Although the cell size was smaller, the Leydig cells (LC) number per testis also significantly increased in iNOS(-/-) mice...
April 18, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28420479/andrographolide-disrupts-meiotic-maturation-by-blocking-cytoskeletal-reorganisation-and-decreases-the-fertilisation-potential-of-mouse-oocytes
#20
Hong-Xing Liang, Sheng-Sheng Lu, Zheng Yan, Yan-Ping Kuang, Xiang-Xing Zhu, Zhi-Guang Yan, Tong Du, Wei-Ran Chai, Hui Long, Qi-Feng Lyu
Andrographolide (AG) is a diterpenoid lactone isolated from the stem and leaves of Andrographis paniculata Nees that is used for the effective treatment of infectious diseases in Asian countries. Previous studies have reported adverse effects of AG on female fertility in rodents; however, the underlying mechanisms are unknown. The aim of the present study was to investigate the effects of AG on the IVM of mouse oocytes and their fertilisation potential. Immature oocytes incubated for 6, 14 or 24h in medium containing 5, 10 or 20μM AG showed time- and dose-dependent decreases in maturation rates compared with the control group...
April 19, 2017: Reproduction, Fertility, and Development
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