keyword
MENU ▼
Read by QxMD icon Read
search

Sperm apoptosis

keyword
https://www.readbyqxmd.com/read/29350306/progress-on-the-study-of-the-mechanism-of-busulfan-cytotoxicity
#1
REVIEW
Xiaoli Chen, Mingyuan Liang, Dong Wang
The preparation of spermatogonial stem cell (SSC) transplant recipients laid the technical foundation for SSC transplant technology and the understanding of spermatogenesis mechanisms. Busulfan is commonly used to prepare recipients for mouse SSC transplantation; however, its safety and efficiency have been questioned. This review summarizes the relationship between SSCs and Sertoli cells (SCs), and the mechanism of busulfan toxicity against sperm cells. We concluded that the proliferation, differentiation, and apoptosis of SSCs are regulated by SCs...
January 19, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29305108/evaluation-of-the-p53-and-thioredoxin-reductase-in-sperm-from-asthenozoospermic-males-in-comparison-to-normozoospermic-males
#2
Mohmmad-Nabi Moradi, Jamshid Karimi, Iraj Khodadadi, Iraj Amiri, Manoochehr Karami, Massoud Saidijam, Akram Vatannejad, Heidar Tavilani
Thioredoxin (Trx) system has a defensive role against the harmful effect of oxidative stress in sperm. p53 is an important regulator of apoptosis and normal process of spermatogenesis. Regulation of p53 by redox state of the cell and Thioredoxin system has been reported. The aim of this study was to evaluate the ROS level, Thioredoxin reductase (TrxR) activity and p53 protein levels in sperm of asthenozoospermic and normozoospermic males. Semen samples from 80 donors were divided into asthenozoospermic (n=40) and normozoospermic (n=40) groups using the WHO criteria...
January 2, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29299971/the-role-of-the-fas-fasl-signaling-pathway-in-environmental-toxicant-induced-testicular-cell-apoptosis-an-update
#3
Mei Wang, Ping Su
The Fas/FasL signaling pathway is one of the major pathways that regulate apoptosis. Increasing studies have shown that the activation of the Fas/FasL signaling pathway is closely associated with testicular cell apoptosis. However, the mechanism involved is still unclear. We discuss recent findings regarding the molecular mechanisms by which environmental toxicants induce testicular pathology via Fas/FasL signaling. These findings suggest that Fas/FasL signaling is employed to impact the sensitivity (a response to external factors) of germ cells, disrupt steroidogenic hormone and cytokine metabolism mediated by Sertoli cells, and elicit the activation of NFAT (nuclear factor of activated T-cells) in Leydig cell apoptosis...
January 4, 2018: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29279200/proteomic-analyses-of-ram-ovis-aries-testis-during-different-developmental-stages
#4
Zengkui Lu, Youji Ma, Quanwei Zhang, Xingxu Zhao, Yong Zhang, Liping Zhang
Male reproductive capacity is essential for animal breeding and reproduction. In males, the testes produce sperm and secrete androgen, processes which require precise regulation by multiple proteins. The composition of proteins in the ram testes has not yet been studied systematically, thus, the application of proteomics to explore differential protein regulation during ram testes development is of great significance. In the present study, ram testes were studied at five different developmental phases to assess postnatal differences in protein regulation...
December 21, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29274354/ochratoxin-a-exposure-decreased-sperm-motility-via-the-ampk-and-pten-signaling-pathways
#5
Tian-Yu Zhang, Rui-Ying Wu, Yong Zhao, Chun-Shuang Xu, Wei-Dong Zhang, Wei Ge, Jing Liu, Zhong-Yi Sun, Shu-Hua Zou, Wei Shen
Ochratoxin A (OTA), a common mycotoxin found in nature, has been implicated as effecting the function of male reproductive systems. OTA exposure has been shown to decrease sperm production and quality, however, the underlying mechanisms remain unknown. In the current investigation boar sperm exposed to 10 and 100μM OTA in vitro for 24h resulted in significantly decreased motility, in the 100μM OTA treatment group when compared with the control group. The level of reactive oxygen species (ROS) was significantly increased in both of the OTA treatment groups...
December 20, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29273675/vitamin-e-and-lycopene-reduce-coal-burning-fluorosis-induced-spermatogenic-cell-apoptosis-via-oxidative-stress-mediated-jnk-and-erk-signaling-pathways
#6
Yuan Tian, Yuehai Xiao, Bolin Wang, Chao Sun, Kaifa Tang, Fa Sun
Although fluoride has been widely used in toothpaste, mouthwash, and drinking water to prevent dental caries, the excessive intake of fluoride can cause fluorosis which is associated with dental, skeletal, and soft tissue fluorosis. Recent evidences have drawn the attention to its adverse effects on male reproductive system that include spermatogenesis defect, sperm count loss, and sperm maturation impairment. Fluoride induces oxidative stress through the activation of mitogen activated protein kinase (MAPK) cascade which can lead to cell apoptosis...
December 22, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/29258708/a-simple-flow-cytometry-protocol-to-determine-simultaneously-live-dead-and-apoptotic-stallion-spermatozoa-in-fresh-and-frozen-thawed-samples
#7
M C Gil, C Ortega Ferrusola, L Anel-López, J M Ortiz-Rodriguez, M Alvarez, P de Paz, L Anel, F J Peña
Spermatozoa undergo apoptotic changes during the cryopreservation process. These changes, recently termed spermptosis, resemble the cryopreservation induced delayed onset of cell death observed after thawing of somatic cells. Due to its importance in cryobiology, methods to easily identify spermptotic cells are warranted. In this study, a well-validated method for identification of spermatozoa with caspase 3 activity was compared with use of the combination of Hoechst 33342 (H-42) and ethidium homodimer (Eth-1)...
December 16, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29179259/roles-of-histone-h3-5-in-human-spermatogenesis-and-spermatogenic-disorders
#8
K Shiraishi, A Shindo, A Harada, H Kurumizaka, H Kimura, Y Ohkawa, H Matsuyama
Histone H3.5 (H3.5) is a newly identified histone variant highly expressed in the human testis. We have reported the crystal structure, instability of the H3.5 nucleosome and accumulation around transcription start sites, mainly in primary spermatocytes, but its role in human spermatogenesis remains poorly understood. Testicular biopsy specimens from 30 men (mean age: 35 years) with non-obstructive azoospermia (NOA) who underwent microdissection testicular sperm extraction and 23 men with obstructive azoospermia (OA) were included...
November 27, 2017: Andrology
https://www.readbyqxmd.com/read/29177237/the-etiologies-of-dna-abnormalities-in-male-infertility-an-assessment-and-review
#9
REVIEW
Soheila Pourmasumi, Parvin Sabeti, Tahereh Rahiminia, Esmat Mangoli, Nasim Tabibnejad, Ali Reza Talebi
The sperm DNA damage may occur in testis, genital ducts, and also after ejaculation. Mechanisms altering chromatin remodeling are abortive apoptosis and oxidative stress resulting from reactive oxygen species. Three classifications of intratesticular, post-testicular, and external factors have been correlated with increased levels of human sperm DNA damage which can affect the potential of fertility. Lifestyle, environment, medical, and iatrogenic factors might be considered to cause dysmetabolism to make distracting interactions and endocrine disrupting compounds...
June 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29138814/grape-seed-proanthocyanidin-extract-attenuates-varicocele%C3%A2-induced-testicular-oxidative-injury-in-rats-by-activating-the-nrf2%C3%A2-antioxidant-system
#10
Yong Wang, Fan Chen, Ming Liang, Shouzhen Chen, Yaofeng Zhu, Zhichuan Zou, Benkang Shi
The present study investigated whether grape seed proanthocyanidin extract (GSPE) can attenuate varicocele‑induced testicular oxidative injury through the nuclear factor (erythroid‑derived 2)‑like 2 (Nrf2) antioxidant pathway. A varicocele model in rats was established by partial ligation of the left renal vein. Following 4 weeks of GSPE administration, the decreased sperm count and motility and other pathological changes caused by varicocele were significantly alleviated, as indicated by the results of computer‑assisted sperm analysis and hematoxylin and eosin staining...
November 10, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29119857/mir-638-inhibits-immature-sertoli-cell-growth-by-indirectly-inactivating-pi3k-akt-pathway-via-spag1-gene
#11
Pandi Hu, Kaifeng Guan, Yue Feng, Changping Ma, Huibin Song, Yang Li, Xuanyan Xia, Jialian Li, Fenge Li
Numerous studies have demonstrated that microRNAs (miRNAs) play important roles in cell growth, apoptosis and spermatogenesis. Our previous study showed that miR-638 was differentially expressed in sexually immature and mature testes of Large White boars. Here we reported that sperm-associated antigen 1 (SPAG1) was a direct target gene of miR-638. Moreover, miR-638 inhibited cell proliferation and cell cycle, and promoted apoptosis of porcine immature Sertoli cells. Key genes including phosphorylated phosphatidylinositide 3-kinases (p-PI3K) and phosphorylated serine/ threonine kinase (p-AKT) in PI3K/AKT pathway as well as cell cycle factors including c-MYC, cyclin-D1 (CCND1), cyclin-E1 (CCNE1) and cyclin-dependent kinase 4 (CDK4) were all significantly down-regulated after overexpression of miR-638 or RNAi of SPAG1...
November 9, 2017: Cell Cycle
https://www.readbyqxmd.com/read/29098592/genotoxicity-of-carbon-tetrachloride-and-the-protective-role-of-essential-oil-of-salvia-officinalis-l-in-mice-using-chromosomal-aberration-micronuclei-formation-and-comet-assay
#12
Kawthar Ae Diab, Maha A Fahmy, Zeinab M Hassan, Emad M Hassan, Adel B Salama, Enayat A Omara
The present work was conducted to evaluate the genotoxic effect of carbon tetrachloride (CCl4) in mouse bone marrow and male germ cells. The safety and the modulating activity of sage (Salvia officinalis L.) essential oil (SEO) against the possible genotoxic effect of CCl4 were also evaluated. A combination of in vivo mutagenic endpoints was included: micronucleus (MN), apoptosis using dual acridine orange/ethidium bromide (AO/EB) staining, comet assay, chromosomal aberrations (CAs), and sperm abnormalities...
November 3, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29091894/zinc-alogliptin-combination-attenuates-testicular-toxicity-induced-by-doxorubicin-in-rats-role-of-oxidative-stress-apoptosis-and-tgf-%C3%AE-1-nf-%C3%AE%C2%BAb-signaling
#13
Ahmed M Kabel
The aim of this study was to assess the effect of alogliptin and/or zinc on doxorubicin (DOX)-induced testicular toxicity in rats. Sixty male Wistar rats were divided into 6 equal groups: Control; DOX; DOX+Zinc; DOX+Alogliptin; DOX+Carboxymethyl cellulose and DOX+Zinc+Alogliptin. Testis weight, testicular functions, serum testosterone, luteinizing hormone, follicle stimulating hormone and zinc were measured. Also, testicular tissue zinc, 3 β-hydroxysteroid dehydrogenase, 17 β- hydroxysteroid dehydrogenase, antioxidant enzymes, pro-inflammatory cytokines, transforming growth factor beta 1 (TGF-β1), nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and sperm characteristics were assessed...
October 27, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29069489/effects-of-intraperitoneal-injection-of-sns2-flowers-on-mouse-testicle
#14
Disi Bai, Qingzhao Li, Yanjie Xiong, Junjian Zhao, Liyuan Bai, Peijun Shen, Lu Yuan, Ping Wu
SnS 2 nanoflowers (SnS 2 NFs) have been widely used in photoelectric and catalytic applications. However, its explosure and reproductive toxicity is unknown. The aim of this study was to investigate the effect of exposure to three different sized-SnS 2 flowers (dose: 38 mg/Kg; size: 50, 80 and 200 nm) in testes of mice for 4 weeks by intraperitoneal injection. Though the body weight of mice treated or not with SnS 2 NFs was not different, and SnS 2 NFs were distributed to the organs including liver, kidney, spleen, heart, brain and testis, more distribution SnS 2 NFs (50 and 80 nm) were found in testicle tissues compared with SnS 2 flowers (200 nm) in those tissues...
October 23, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29068181/semen-parameters-in-men-with-varicocele-dna-fragmentation-chromatin-packaging-mitochondrial-membrane-potential-and-apoptosis
#15
Felipe Dieamant, Claudia G Petersen, Ana L Mauri, Vanessa Conmar, Mariana Mattila, Laura D Vagnini, Adriana Renzi, Bruna P Costa, Camila Zamara, João Batista A Oliveira, Ricardo L R Baruffi, José G Franco
OBJECTIVE: To evaluate varicocele effects on conventional semen parameters: sperm DNA fragmentation, chromatin packaging, mitochondrial membrane potential (MMP) and sperm apoptosis. METHODS: A cross-sectional study was carried out with semen samples from 2,399 men of couples who attended an infertility clinic. A total of 16.3% (391/2399) of the men were diagnosed with varicocele by a urologist. RESULTS: A regression analysis revealed that the percentages of sperm with DNA fragmentation, abnormal chromatin packaging, and abnormal MMP were significantly increased in individuals with varicocele, when compared to men without varicocele...
October 25, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29066868/protective-effect-of-da-9401-in-finasteride-induced-apoptosis-in-rat-testis-inositol-requiring-kinase-1-and-c-jun-n-terminal-kinase-pathway
#16
Kiran Kumar Soni, Yu Seob Shin, Bo Ram Choi, Keshab Kumar Karna, Hye Kyung Kim, Sung Won Lee, Chul Young Kim, Jong Kwan Park
Finasteride is used to treat male pattern baldness and benign prostatic hyperplasia. This study investigated the toxicity of finasteride and recovery by DA-9401 using Sprague Dawley (SD) rats. Forty adult male SD rats were assigned to four groups: control (CTR), finasteride 1 mg/kg/day (F), finasteride 1 mg/kg + DA-9401 100 mg/kg/day (F + DA 100) and finasteride 1 mg/kg + DA-9401 200 mg/kg/day (F + DA 200). Treatments were by oral delivery once daily for 90 consecutive days. The gross anatomical parameters assessed included: genital organ weight; vas deferens sperm count and sperm motility; testosterone, dihydrotestosterone (DHT) and malondialdehyde levels; and histological and terminal deoxynucleotidyl transferase enzyme mediated dUTP nick-end labeling (TUNEL) staining of testis for spermatogenic cell density, Johnsen's score and apoptosis...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29053706/solea-senegalensis-sperm-cryopreservation-new-insights-on-sperm-quality
#17
Marta F Riesco, Catarina Oliveira, Florbela Soares, Paulo J Gavaia, María T Dinis, Elsa Cabrita
Cryopreservation of Senegalese sole sperm can represent an alternative to overcome some reproductive problems of this species. However, it is important to guarantee the safe use of cryopreserved sperm by selecting an appropriate protocol according to a high demand quality need to be ensured. It has been demonstrated that traditional assays such as motility and viability do not provide enough information to identify specific damage caused by cryopreservation process (freezing and thawing). Specific tests, including lipid peroxidation and DNA damage, should be performed...
2017: PloS One
https://www.readbyqxmd.com/read/29044638/impact-of-melatonin-supplementation-in-the-rat-spermatogenesis-subjected-to-forced-swimming-exercise
#18
A Moayeri, T Mokhtari, A Hedayatpour, H-A Abbaszadeh, S Mohammadpour, H Ramezanikhah, S Shokri
Oxygen consumption increases many times during exercise, which can increase reactive oxygen species. It negatively affects fertility in male athletes. Melatonin is exerting a regulatory role at different levels of the hypothalamic-pituitary-gonadal axis. However, there is no evidence that the protective effects of melatonin persist after long duration exercise on the spermatogenesis. Therefore, this study was conducted to examine the impacts of melatonin on the testis following the administration of swimming exercise...
October 18, 2017: Andrologia
https://www.readbyqxmd.com/read/29042680/sperm-borne-mir-449b-influences-cleavage-epigenetic-reprogramming-and-apoptosis-of-scnt-embryos-in-bovine
#19
Mengyun Wang, Yang Gao, Pengxiang Qu, Suzhu Qing, Fang Qiao, Yong Zhang, Jesse Mager, Yongsheng Wang
Accumulating evidence indicates the absence of paternally derived miRNAs, piwiRNAs, and proteins may be one important factor contributing to developmental failure in somatic cell cloned embryos. In the present study, we found microRNA-449b (miR-449b) was highly expressed in sperm. Target gene predictions and experimental verification indicate that several embryonic development-related genes, including CDK6, c-MYC, HDAC1 and BCL-2, are targets of miR-449b. We therefore investigated the role of miR-449b using somatic cell nuclear transfer (SCNT) embryo model...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29030185/inhibitory-effect-of-tartrate-against-phosphate-induced-dj-1-aggregation
#20
Min Soo Kim, Sangmin Lee, Sanguk Yun, Pann-Ghill Suh, Jongmi Park, Minghua Cui, Sun Choi, Sun-Shin Cha, Wook Jin
The DJ-1 protein engages in diverse cellular and pathological processes, including tumorigenesis, apoptosis, sperm fertilization, and the progression of Parkinson's disease (PD). The functional dimeric form of DJ-1 transforms into non-functional filamentous aggregates in an inorganic phosphate (Pi)-dependent manner in vitro. Here, we demonstrated that Pi and reactive oxygen species (ROS) induce DJ-1 aggregation in Neuro2A and SH-SY5Y cells. Remarkably, tartrate treatment significantly reduced Pi- and ROS-induced DJ-1 aggregation and restored Pi- and ROS-provoked cell death using quantitative data as mean±standard deviation, and statistics...
October 10, 2017: International Journal of Biological Macromolecules
keyword
keyword
104261
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"