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Sperm apoptosis

Meijiao Wang, Tinghe Yu, Lina Hu, Zhi Cheng, Min Li
Ubiquitin C-terminal hydrolase L3 (UCHL3) belongs to the group of deubiquitinating enzymes and plays a part in apoptosis of germ cells and the differentiation of spermatocytes into spermatids. However, the exact role of UCHL3 in human spermatogenesis and sperm function remains unknown. Here we examined the level and activity of UCHL3 in spermatozoa from men with asthenozoospermia (A), oligoasthenozoospermia (OA) or normozoospermia (N). Immunofluorescence indicated that UCHL3 was mainly localized in the acrosome and throughout the flagella, and western blotting revealed a lower level in A or OA compared with N (p < 0...
2016: PloS One
Pan Huang, Zhengrong Zhou, Fangxiong Shi, Genbao Shao, Ran Wang, Jintian Wang, Kangxin Wang, Wei Ding
The aim of the present study was to determine the effects of the insulin-like growth factor 1 (IGF-1)/phosphatase and tensin homologue deleted on chromosome 10 (PTEN)/Akt/forkhead box (FoxO) signaling pathway on male reproduction in rats subjected to water immersion and restraint stress (WRS). Sperm morphology, sperm malformation rate, and serum testosterone concentration were analyzed following WRS. In addition, the expression levels and immunolocalization of IGF‑1, PTEN, Akt and FoxO proteins, as well as the rate of cell apoptosis in rat testes, were investigated...
October 24, 2016: Molecular Medicine Reports
Qing Chen, Xin Zhang, Jin-Yan Zhao, Xiao-Ning Lu, Peng-Sheng Zheng, Xiang Xue
The effects of paraquat (PQ) on the male reproductive system are unclear. In this study, male rats were divided into four groups (0, 0.5, 2, and 8 mg/kg) and treated with PQ by oral gavage for 8 weeks. At the end of the experiment, a significant decline in sperm count, motility, and viability and an increase in teratospermia were observed in the PQ-treated group (P < 0.05). Further investigation found that PQ resulted in enhanced lipid peroxidation and more apoptosis in the testis tissues, and apoptosis was likely to be associated with activation of the mitochondrial pathway...
October 20, 2016: Journal of Biochemical and Molecular Toxicology
Miao Long, Shuhua Yang, Yi Zhang, Peng Li, Jianxin Han, Shuang Dong, Xinliang Chen, Jianbin He
Zearalenone (ZEN) exerts a major effect on human and animal health and has led to serious worldwide economic problems. In this study, we investigated whether proanthocyanidin (PC) can prevent ZEN-induced testicular oxidative damage in male mice and explored the underlying mechanism. Kunming mice were injected with ZEN (40 mg kg(-1)) on the 11th day after intragastric administration of PC (75 or 150 mg/kg) for 10 days; the sperm quality of mice was then analysed statistically. Additionally, testicular morphology parameters related to oxidative damage, apoptosis and the expression of endoplasmic reticulum (ER) stress-related genes (GRP78, CHOP and XBP-1) were all measured...
October 19, 2016: Environmental Science and Pollution Research International
Ryan Dean Fortune, Raymond J Grill, Christine Beeton, Mark Tanner, Redwan Huq, David S Loose
Spinal cord injury (SCI) results in devastating changes to almost all aspects of a patient's life. In addition to a permanent loss of sensory and motor function, males will also frequently exhibit a profound loss of fertility through poorly understood mechanisms. We demonstrate that SCI causes measureable pathology in the testis both acutely (24 hours) and chronically, up to 1.5 years post injury, leading to loss in sperm motility and viability. SCI has been shown in humans and rats to induce leukocytospermia, with the presence of inflammatory cytokines, anti-sperm antibodies, and reactive oxygen species found within the ejaculate...
October 18, 2016: Journal of Neurotrauma
Xiangrong Cui, Xuan Jing, Xueqing Wu, Meiqin Yan
Male infertility is a complex, multifactorial and polygenic disease that contributes to ~50% cases of infertility. Previous studies have demonstrated that excess weight and obesity factors serve an important role in the development of male infertility. An increasing number of studies have reported that resveratrol may regulate the response of cells to specific stimuli that induce cell injury, as well as decrease germ cell apoptosis in mice or rats. In the present study, the semen quality and serum sex hormone levels were evaluated in 324 men, which included 73 underweight, 82 normal weight, 95 overweight and 74 obese men...
October 12, 2016: Molecular Medicine Reports
Fayez A Almabhouh, Khairul Osman, Siti Fatimah Ibrahim, Sergey Gupalo, Justin Gnanou, Effendi Ibrahim, Harbindar Jeet Singh
This study examined the effects of melatonin on leptin-induced changes in sperm parameters in adult rats. Five groups of Sprague-Dawley rats were treated with either leptin or leptin and melatonin or melatonin for 6 weeks. Leptin was given daily via the intraperitoneal route (60 μg kg-1 body weight) and melatonin was given in drinking water (10 mg kg-1 or 20 mg kg-1 body weight per day). Upon completion, sperm count, sperm morphology, 8-hydroxy-2-deoxyguanosine, Comet assay, TUNEL assay, gene expression profiles of antioxidant enzymes, respiratory chain reaction enzymes, DNA damage, and apoptosis genes were estimated...
October 14, 2016: Asian Journal of Andrology
Fu Mei, Xu Kehui, Xu Wenming
Dicer, an RNAse III endonuclease, is critical for the biogenesis of small noncoding RNAs (microRNAs), including the biogenesis of microRNAs and small interfering RNAs, which transcriptionally and post-transcription ally regulate mRNA expression through binding to target mRNA and leading to subsequent mRNA degradation. Recent studies show that Dicer plays important roles in cell proliferation, differentiation and apoptosis. It has been attracted more and more attention in the reproductive field. In the male reproduction field, mouse model shows that Dicer is critical for the development of spermatogenic cell, sperm maturation, sperm motility and morphology...
July 20, 2016: Yi Chuan, Hereditas
Łukasz Jarosz, Zbigniew Grądzki, Marcin Kalinowski, Ewa Laskowska
BACKGROUND: The aim of the study was to evaluate the quality of fresh raccoon dog semen and raccoon dog semen stored at 4 °C. The qualitative evaluation was based on apoptosis in the sperm cells, which was tested by the Annexin V/Pi assay, the TUNEL method and JC-1. In addition, the suitability of the semen for insemination and its effect on reproduction in females were determined in relation to the time of storage. RESULTS: During cold storage of the semen, in the samples from all groups a gradual decrease was noted in the percentage of live cells, and an increase in the percentage of cells with abnormal morphology, exhibiting changes typical of late-stage apoptosis (V(+)/PI(+)), and of necrotic cells (V(-)/PI(+))...
October 10, 2016: BMC Veterinary Research
Atefeh Najafi, Ebrahim Asadi, Adel R Moawad, Saideh Mikaeili, Fardin Amidi, Emmanuel Adutwum, Majid Safa, Ali Gholi Sobhani
OBJECTIVE: To investigate the effects of brain-derived neurotrophic factor (BDNF) supplementation to freezing and thawing media on frozen-thawed human sperm parameters. DESIGN: Laboratory study. SETTING: University hospital. PATIENT(S): Semen samples from 21 healthy fertile men. INTERVENTION(S): We measured reactive oxygen species (ROS) by flow cytometry using the probes dichlorofluorescin diacetate for intracellular hydrogen peroxide (H2O2) and dihydroethidium for intracellular superoxide anion (O2(-•)), sperm plasma membrane integrity by flow cytometry, caspase-3 activity using ELISA, and AKT phosphorylation status using Western blot in sperm that was cryopreserved and thawed in media either supplemented with BDNF or without BDNF supplementation (control)...
October 4, 2016: Fertility and Sterility
Robert Janowski, Sandra Scanu, Dierk Niessing, Tobias Madl
The mammalian glutathione peroxidase (GPx) family is a key component of the cellular antioxidative defence system. Within this family, GPx4 has unique features as it accepts a large class of hydroperoxy lipid substrates and has a plethora of biological functions, including sperm maturation, regulation of apoptosis and cerebral embryogenesis. In this paper, the structure of the cytoplasmic isoform of mouse phospholipid hydroperoxide glutathione peroxidase (O70325-2 GPx4) with selenocysteine 46 mutated to cysteine is reported solved at 1...
October 1, 2016: Acta Crystallographica. Section F, Structural Biology Communications
Ala Abdelali, Maie Al-Bader, Narayana Kilarkaje
Diabetes induces oxidative stress, DNA damage and alters several intracellular signaling pathways in organ systems. This study investigated modulatory effects of Trans-Resveratrol on type 1 diabetes mellitus (T1DM)-induced abnormal spermatogenesis, DNA damage and alterations in poly (ADP-ribose) polymerase (PARP) signaling in rat testis. Trans-Resveratrol administration (5mg/kg/day, ip) to Streptozotocin-induced T1DM adult male Wistar rats from day 22-42 resulted in recovery of induced oxidative stress, abnormal spermatogenesis and inhibited DNA synthesis, and led to mitigation of 8-hydroxy-2'-deoxyguanosine formation in the testis and spermatozoa, and DNA double-strand breaks in the testis...
September 27, 2016: Toxicology and Applied Pharmacology
Ya-Guang Sun, Yi-Ning Sun, Li-Xin You, Yi-Nan Liu, Fu Ding, Bao-Yi Ren, Gang Xiong, Valerian Dragutan, Ileana Dragutan
New cytotoxic, mononuclear Pt and Pd coordination complexes featuring the planar, multi-donor ligand (2,3-f)pyrazino(1,10)phenanthroline-2,3-dicarboxylic acid (H2PPDA) have been synthesized under hydrothermal conditions (in water, at high temperature and pressure) and fully characterized. The complexes were proven to be isostructural by applying the single-crystal X-ray diffraction technique. UV-Vis and fluorescence spectroscopy investigations on their interaction with fish sperm DNA revealed a considerable binding capacity while gel electrophoresis provided evidence in favor of cleavage of pBR322 plasmid DNA...
September 14, 2016: Journal of Inorganic Biochemistry
Si-Qi Yan, Rui Xing, Yan-Feng Zhou, Kai-Le Li, Yuan-Yuan Su, Jian-Feng Qiu, Yun-Hu Zhang, Ke-Qin Zhang, Yao He, Xiao-Ping Lu, Shi-Qing Xu
Sexual glands are key sites affected by nanotoxicity, but there is no sensitive assay for measuring reproductive toxicity in animals. The aim of this study was to investigate the toxic effects of cadmium telluride quantum dots (CdTe-QDs) on gonads in a model organism, Bombyx mori. After dorsal vein injection of 0.32 nmol of CdTe-QDs per individual, the QDs passed through the outer membranes of gonads via the generation of ROS in the membranes of spermatocysts and ovarioles, as well as internal germ cells, thereby inducing early germ cell death or malformations via complex mechanisms related to apoptosis and autophagy through mitochondrial and lysosomal pathways...
September 27, 2016: Scientific Reports
Dong Lianhua, Ran Maoliang, Li Zhi, Peng Fuzhi, Chen Bin
Ubiquitin-proteasome pathway (UPP) is the main pathway of protein degradation in eukaryotic cells. The UPP plays very important roles in cell cycle progression, apoptosis, stress response and growth and development through regulating protein interaction, protein activity, protein localization and signal transduction. Previous studies have shown that the UPP is essential for regulating acrosome and tail biogenesis during spermatogenesis in human and animals. The dysregulation of UPP during spermatogenesis results in sperm deformity and reduced sperm motility and leads to reproductive system diseases such as oligospermatism, infertility and testicular tumors...
September 20, 2016: Yi Chuan, Hereditas
I M Aparicio, J Espino, I Bejarano, A Gallardo-Soler, M L Campo, G M Salido, J A Pariente, F J Peña, J A Tapia
Macroautophagy (hereafter autophagy) is an evolutionarily highly conserved cellular process that participates in the maintenance of intracellular homeostasis through the degradation of most long-lived proteins and entire organelles. Autophagy participates in some reproductive events; however, there are not reports regarding the role of autophagy in the regulation of sperm physiology. Hence, the aim of this study was to investigate whether autophagy-related proteins are present and functionally active in human spermatozoa...
2016: Scientific Reports
Magdalena J Kacperska, Jakub Walenczak, Bartłomiej Tomasik
There is ongoing research with the goal of finding precise and sensitive biomarkers of multiple sclerosis (MS). Recently, researchers have paid particular attention to small, non-encoding, single stranded endogenous microRNA molecules (miR, miRNA). At first these molecules were thought to be found only within the cell. Today it is known, however, that they can also be found in the extracellular spaces (plasma, serum, saliva, urine, tears, sweat, milk, sperm and amniotic fluid, among others). It has been established that extracellular miRNA perform a wide spectrum of functions, such as transmitting signals between cells, modulating processes involved in angiogenesis, neurogenesis, proliferation or apoptosis...
July 2016: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Larissa Berloffa Belardin, Paula Toni Del Giudice, Mariana Camargo, Paula Intasqui, Mariana Pereira Antoniassi, Ricardo Pimenta Bertolla, Agnaldo Pereira Cedenho
PURPOSE: To verify if the presence of varicocele (grades II and III) with and without seminal alterations, using the 5(th) centile cutoff values in table A1.1 of the World Health Organization (WHO, 2010) manual, alters the seminal plasma levels of proteins DNASE1 (deoxyribonuclease-1) and IGFBP7 (Insulin-like growth factor-binding protein 7), which are related to apoptosis regulation and cell proliferation, respectively, demonstrating that these proteins are important for correct spermatogenesis...
September 14, 2016: Journal of Assisted Reproduction and Genetics
Tengfei Liu, Ping Yang, Hong Chen, Yufei Huang, Yi Liu, Yasir Waqas, Nisar Ahmed, Xiaoya Chu, Qiusheng Chen
Important evolutionary and ecological consequences arise from the ability of female turtles to store viable spermatozoa for an extended period. Although previous morphological studies have observed the localization of spermatozoa in Pelodiscus sinensis oviduct, no systematic study on the identification of genes that are involved in long-term sperm storage has been performed. In this study, the oviduct of P. sinensis at different phases (reproductive and hibernation seasons) was prepared for RNA-Seq and gene expression profiling...
2016: Scientific Reports
Ana M S Silva, Sara Correia, João E Casalta-Lopes, Ana C Mamede, José E B Cavaco, Maria F Botelho, Sílvia Socorro, Cláudio J Maia
AIMS: Regucalcin (RGN), a protein broadly expressed in the male reproductive tract, has shown to have beneficial effects on spermatogenesis suppressing chemical-induced apoptosis. This study aimed to evaluate whether RGN overexpression ameliorates the spermatogenic phenotype after radiation treatment. MAIN METHODS: Transgenic rats overexpressing RGN (Tg-RGN) and their wild-type (Wt) counterparts were exposed to a single dose of X-rays (6Gy), and at ten weeks after irradiation, the testicular status and the epididymal sperm parameters were evaluated...
September 9, 2016: Life Sciences
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