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https://www.readbyqxmd.com/read/28533001/evaluation-of-the-rapidec%C3%A2-carba-np-and-%C3%AE-carba%C3%A2-tests-for-rapid-detection-of-carbapenemase-producing-enterobacteriaceae
#1
Stefano Mancini, Nicolas Kieffer, Laurent Poirel, Patrice Nordmann
The analytical performances of the RAPIDEC CARBA NP® (bioMérieux) and the β-CARBA® (Bio-Rad) tests were evaluated for the rapid detection of any known type of carbapenemases in Enterobacteriaceae. An international collection of 149 enterobacterial isolates comprising 111 Carbapenemase-Producing Enterobacteriaceae (CPE) and 38 non-carbapenemase producers was used. CPE included 32 carbapenemase producers of class A (18 KPC-2, 1 FRI-1, 5 SME and 8 IMI), 33 of class B (13 NDM, 11 VIM and 9 IMP) and 46 of class D (15 OXA-48, 14 OXA-181, 10 OXA-204, 3 OXA-232 and 4 OXA-162)...
May 13, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28527193/comparison-of-digital-intraoral-scanners-by-single-image-capture-system-and-full-color-movie-system
#2
Meguru Yamamoto, Yu Kataoka, Atsufumi Manabe
BACKGROUND: The use of dental computer-aided design/computer-aided manufacturing (CAD/CAM) restoration is rapidly increasing. OBJECTIVE: This study was performed to evaluate the marginal and internal cement thickness and the adhesive gap of internal cavities comprising CAD/CAM materials using two digital impression acquisition methods and micro-computed tomography. METHODS: Images obtained by a single-image acquisition system (Bluecam Ver...
2017: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/28526575/percutaneous-electrical-nerve-field-stimulation-modulates-central-pain-pathways-and-attenuates-post-inflammatory-visceral-and-somatic-hyperalgesia-in-rats
#3
Reji Babygirija, Manu Sood, Pradeep Kannampalli, Jyoti N Sengupta, Adrian Miranda
A non-invasive, auricular percutaneous electrical nerve field stimulation (PENFS) has been suggested to modulate central pain pathways. We investigated the effects of BRIDGE® device on the responses of amygdala and lumbar spinal neurons and the development of post-colitis hyperalgesia. Male Sprague-Dawley rats received intracolonic trinitrobenzene sulfonic acid (TNBS) and PENFS on the same day. Control rats had sham devices. The visceromotor response (VMR) to colon distension and paw withdrawal threshold (PWT) was recorded after 7 days...
May 16, 2017: Neuroscience
https://www.readbyqxmd.com/read/28520233/nanojunction-polymer-photoelectrode-for-efficient-charge-transport-and-separation
#4
Qiushi Ruan, Wenjun Luo, Jijia Xie, Yiou Wang, Xu Liu, Zhiming Bai, Claire J Carmalt, Junwang Tang
A novel nanojunction archiecture of metal-free photoanode, composed of B-doped carbon nitride nanolayer and bulk carbon nitride has been fabricated by a one-step construction approach. This type of nanojunction overcomes a few intrinsic drawbacks of carbon nitride film, e.g. severe bulk charge recombination and slow charge transfer. For the optium sample, the top layer of the nanojunction has a depth of ca. 100 nm and the bottom layer is ca. 900 nm. The nanojunction photoanode results into a 10 fold higher photocurrent than bulk graphitic carbon nitride and an extremely high incident photon-to-current efficiency (IPCE) of ca...
May 18, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28511288/a-tele-health-follow-up-strategy-for-tight-control-of-disease-activity-in-rheumatoid-arthritis-results-of-the-non-inferiority-randomised-controlled-trail-the-tera-study
#5
Annette Thurah, Kristian Stengaard-Pedersen, Mette Axelsen, Ulrich Fredberg, Liv M V Schougaard, Niels H I Hjollund, Mogens Pfeiffer-Jensen, Trine B Laurberg, Ulrik Tarp, Kirsten Lomborg, Thomas Maribo
OBJECTIVE: To test the effect of patient-reported outcome (PRO) based tele-health follow-up for tight control of disease activity in patients with rheumatoid arthritis (RA), and the differences between tele-health follow-up performed by rheumatologists or rheumatology nurses. METHODS: A total of 294 patients were randomized (1:1:1) to either PRO-based tele-health follow-up carried out by a nurse (PRO-TN) or a rheumatologist (PRO-TR), or conventional out-patient follow-up by physicians...
May 16, 2017: Arthritis Care & Research
https://www.readbyqxmd.com/read/28508147/identification-of-beta-lactamases-and-beta-lactam-related-proteins-in-human-pathogenic-bacteria-using-a-computational-search-approach
#6
Aniel Jessica Leticia Brambila-Tapia, Ernesto Perez-Rueda, Humberto Barrios, Nory Omayra Dávalos-Rodríguez, Ingrid Patricia Dávalos-Rodríguez, Ernesto Germán Cardona-Muñoz, Mario Salazar-Páramo
A systematic analysis of beta-lactamases based on comparative proteomics has not been performed thus far. In this report, we searched for the presence of beta-lactam-related proteins in 591 bacterial proteomes belonging to 52 species that are pathogenic to humans. The amino acid sequences for 19 different types of beta-lactamases (ACT, CARB, CifA, CMY, CTX, FOX, GES, GOB, IMP, IND, KPC, LEN, OKP, OXA, OXY, SHV, TEM, NDM, and VIM) were obtained from the ARG-ANNOT database and were used to construct 19 HMM profiles, which were used to identify potential beta-lactamases in the completely sequenced bacterial proteomes...
May 16, 2017: Current Microbiology
https://www.readbyqxmd.com/read/28505394/1-2-4-triazole-3-thione-compounds-as-inhibitors-of-di-zinc-metallo-%C3%AE-lactamases
#7
Laurent Sevaille, Laurent Gavara, Carine Bebrone, Filomena De Luca, Lionel Nauton, Maud Achard, Paola Mercuri, Silvia Tanfoni, Luisa Borgianni, Carole Guyon, Pauline Lonjon, Gülhan Turan-Zitouni, Julia Dzieciolowski, Katja Becker, Lionel Bénard, Ciaran Condon, Ludovic Maillard, Jean Martinez, Jean-Marie Frère, Otto Dideberg, Moreno Galleni, Jean-Denis Docquier, Jean-François Hernandez
Metallo-β-lactamases (MBL) cause resistance of Gram-negative bacteria to β-lactam antibiotics and are of serious concern because they can inactivate the last-resort carbapenems and because MBL inhibitors of clinical value are still lacking. We have previously identified the original binding mode of a 4-amino-1,2,4-triazole-3-thione compound, IIIA, within the di-zinc active site of the L1 MBL. Here, we present the crystallographic structure of a complex of L1 with the corresponding non-amino compound IIIB...
May 15, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28485708/molecular-study-of-carbapenemase-genes-in-clinical-isolates-of-enterobacteriaceae-resistant-to-carbapenems-and-determining-their-clonal-relationship-using-pulsed-field-gel-electrophoresis
#8
Fereshteh Shahcheraghi, Mohammad Mehdi Aslani, Hassan Mahmoudi, Zahra Karimitabar, Hamid Solgi, Abbas Bahador, Mohammad Yousef Alikhani
PURPOSE: Enterobacteriaceae is a large family of Gram-negative bacteria that are considered as normal gut flora. They are the most common human pathogens. The main objective of this study was to investigate the carbapenemase genes in clinical isolates of Enterobacteriaceae resistant to carbapenem antibiotics and determine their clonal relationship using pulsed-field gel electrophoresis (PFGE). METHODOLOGY: In the present study, bacteria were isolated and identified via conventional biochemical tests and API 20NE...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28476242/evaluation-of-the-carbapenem-inactivation-method-cim-for-detecting-carbapenemase-activity-in-enterobacteria
#9
A Aguirre-Quiñonero, M E Cano, D Gamal, J Calvo, L Martínez-Martínez
OBJECTIVES: The objective of this study was to evaluate the accuracy of the CIM test in the detection of carbapenemase activity in 124 strains of Enterobacteriaceae. METHODS: A panel of 124 previously characterized Enterobacteriaceae was tested: 77 strains producing the following carbapenemase families: KPC (n = 14), GES (n = 22), NDM (n = 19), VIM (n = 4), IMP (n = 4) and OXA-48 (n = 14) and 47 non-carbapenemase producers. For the CIM method, an active susceptibility meropenem disc was exposed to a bacterial suspension of a test strain; when a carbapenemase is produced, the antibiotic is inactivated allowing uninhibited growth of an indicator strain after overnight incubation...
March 16, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28467588/the-effect-of-2-different-surgical-methods-on-intracompartmental-pressure-value-in-tibial-shaft-fracture-an-experimental-study-in-a-rabbit-model
#10
Cemil Ertürk, Mehmet Akif Altay, Nuray Altay, İbrahim Avşin Öztürk, İslam Baykara, Cemil Sert, Uğur Erdem Işıkan
BACKGROUND: Intracompartmental pressure (ICP) monitoring is a widely used modality, particularly after intramedullary nailing of tibial shaft fractures. It was hypothesized that ICP value in fracture fixed with Ilizarov circular fixator (ICF) might be lower than in fracture fixed with intramedullary pin (IMP). The present study is a comparison of ICP value in tibial fractures in a rabbit model fixed with ICF and IMP. METHODS: Twenty male New Zealand White rabbits were randomly divided into 2 groups of equal size: ICF group (Group 1) and IMP group (Group 2)...
March 2017: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
https://www.readbyqxmd.com/read/28466083/electrovariable-gold-nanoparticle-films-at-liquid-liquid-interfaces-from-redox-electrocatalysis-to-marangoni-shutters
#11
Grégoire C Gschwend, Evgeny Smirnov, Pekka Peljo, Hubert H Girault
Control over the physical properties of nanoparticle assemblies at a liquid-liquid interface is a key technological advancement to realize the dream of smart electrovariable nanosystems. Electrified interfaces, such as the interface between two immiscible electrolytes solutions (ITIES), are almost an ideal platform for realizing this dream. Here, we show that the Galvani potential difference across soft interfaces can be effectively used to manipulate: (i) the reactivity of gold nanoparticle assemblies through varying the Fermi level (both chemically and electrochemically); (ii) the location distribution of the nanoparticles at the liquid-liquid interface...
May 3, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28460967/optimal-experimental-design-for-discriminating-between-microbial-growth-models-as-function-of-suboptimal-temperature-from-in-silico-to-in-vivo
#12
I Stamati, S Akkermans, F Logist, E Noriega, J Van Impe
Temperature is an important food preservation factor, affecting microbial growth. Secondary predictive models can be used for describing the impact of this factor on microbial growth. In other words, the microbial behavior can be described in a dynamic environment with the use of a primary and secondary model. Two models for describing the effect of temperature on the microbial growth rate are the cardinal temperature model with inflection (CTMI) (Rosso et al., 1993) and its adapted version (aCTMI) (Le Marc et al...
November 2016: Food Research International
https://www.readbyqxmd.com/read/28456489/evaluation-of-commercial-phenotypic-assays-for-the-detection-of-imp-or-new-delhi-metallo-%C3%AE-lactamase-producing-enterobacteriaceae-isolates-in-japan
#13
Daisuke Sakanashi, Makoto Kawachi, Yuki Uozumi, Mitsuru Nishio, Yuki Hara, Hiroyuki Suematsu, Mao Hagihara, Naoya Nishiyama, Nobuhiro Asai, Yusuke Koizumi, Yuka Yamagishi, Hiroshige Mikamo
OBJECTIVES: This study was designed to evaluate the sodium mercaptoacetic acid double disk synergy test (SMA-DDST), the Etest metallo-β-lactamase (MBL) MP/MPI (Etest MP/MPI), and the Mastdiscs ID Carbapenemase Detection Disc Set (MAST-CDS) for the detection of MBL-producing Enterobacteriaceae isolates in Japan. METHODS: Fifty-one clinical isolates and four reference strains were tested. These isolates included 40, 4, and 11 IMP-, New Delhi MBL (NDM)-, and non-MBL-producers, respectively...
April 26, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28452334/dosimetric-effect-of-internal-metallic-ports-in-temporary-tissue-expanders-on-postmastectomy-radiation-therapy-a-monte-carlo-study
#14
Jong Min Park, Kyubo Kim, Jong In Park, Kyung Hwan Shin, Ung Sik Jin, Jung-In Kim
To investigate the dosimetric effect of the internal metallic port (IMP) in a tissue expander (TE) on the dose distribution of postmastectomy radiation therapy (PMRT). A total of 10 patients who have received PMRT with a TE were selected retrospectively. For each patient, the dose distributions of treatment plans with a 10 MV photon beam were calculated using the Monte Carlo (MC) method with CT images. The dose distributions without the TE were also calculated by designating the mass densities of the TE including the IMP as those of tissue...
April 28, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28451566/an-insight-to-how-estate-women-in-sri-lanka-manage-abnormal-vaginal-discharge
#15
Ilankoon Imps, Goonewardena Cse, Fernandopulle Rc, Perera Ppr
No abstract text is available yet for this article.
February 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28449851/acute-compartment-syndrome
#16
Andrew H Schmidt
Acute compartment syndrome is a well-known complication of tibial fractures, yet it remains difficult to diagnose and the only effective treatment is surgical fasciotomy. Delayed fasciotomy is the most important factor contributing to poor outcomes, and as a result, treatment is biased towards performing early fasciotomy. Current diagnosis of ACS is based on clinical findings and intramuscular pressure (IMP) measurement, and is targeted at identifying safe thresholds for when fasciotomy can be avoided. Since clinical findings are variable and difficult to quantify, measurement of IMP - ideally continuously - is the cornerstone of surgical decision - making...
April 24, 2017: Injury
https://www.readbyqxmd.com/read/28448888/isolation-identification-and-characterization-of-potential-impurities-of-anidulafungin
#17
Lanning Zhao, Qilong Wang, Yi Bie, Xiaoxia Lu
Eight impurities ranging from 0.03 to 0.97% in anidulafungin bulk drug were detected by HPLC. Four impurities (Imp-I, Imp-II, Imp-III and Imp-VIII) among impurities were isolated from the self-prepared or marketed samples of anidulafungin bulk drug by means of preparative HPLC. A thorough study was undertaken to characterize these impurities and based on 1D ((1)H, (13)C, H-D, DEPT 90 and 135) and 2D (COSY, TOCSY, HSQC, HMBC) NMR and ESI-MS spectral data. Based on the characterization data, Imp-I was found to be known open-chain hydrolysis product formed during the synthesis and degradation...
April 17, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28445009/evaluation-of-six-phenotypic-methods-for-the-detection-of-carbapenemases-in-gram-negative-bacteria-with-characterized-resistance-mechanisms
#18
Kunling Sun, Xiuyu Xu, Jinrong Yan, Liping Zhang
BACKGROUND: We compared the performance of the modified Hodge test (MHT), Triton Hodge test (THT), Carba NP test (CNPt), simplified Carba NP test (CNPt-direct), blue-Carba NP test (BCT), and carbapenem inactivation method (CIM) for rapid and accurate carbapenemase detection. METHODS: The methods were evaluated by using 256 gram-negative isolates, including 197 Enterobacteriaceae (79 Enterobacter spp., 74 Klebsiella spp., 33 Escherichia coli, 10 Citrobacter spp., and 1 Serratia marcescens), 51 Acinetobacter baumannii, and 8 Pseudomonas aeruginosa strains...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28444620/environmental-pollution-with-antimicrobial-agents-from-bulk-drug-manufacturing-industries-in-hyderabad-south-india-is-associated-with-dissemination-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-pathogens
#19
Christoph Lübbert, Christian Baars, Anil Dayakar, Norman Lippmann, Arne C Rodloff, Martina Kinzig, Fritz Sörgel
PURPOSE: High antibiotic and antifungal concentrations in wastewater from anti-infective drug production may exert selection pressure for multidrug-resistant (MDR) pathogens. We investigated the environmental presence of active pharmaceutical ingredients and their association with MDR Gram-negative bacteria in Hyderabad, South India, a major production area for the global bulk drug market. METHODS: From Nov 19 to 28, 2016, water samples were collected from the direct environment of bulk drug manufacturing facilities, the vicinity of two sewage treatment plants, the Musi River, and habitats in Hyderabad and nearby villages...
April 26, 2017: Infection
https://www.readbyqxmd.com/read/28440342/significant-variation-between-snp-based-hla-imputations-in-diverse-populations-the-last-mile-is-the-hardest
#20
D J Pappas, A Lizee, V Paunic, K R Beutner, A Motyer, D Vukcevic, S Leslie, J Biesiada, J Meller, K D Taylor, X Zheng, L P Zhao, P-A Gourraud, J A Hollenbach, S J Mack, M Maiers
Four single nucleotide polymorphism (SNP)-based human leukocyte antigen (HLA) imputation methods (e-HLA, HIBAG, HLA*IMP:02 and MAGPrediction) were trained using 1000 Genomes SNP and HLA genotypes and assessed for their ability to accurately impute molecular HLA-A, -B, -C and -DRB1 genotypes in the Human Genome Diversity Project cell panel. Imputation concordance was high (>89%) across all methods for both HLA-A and HLA-C, but HLA-B and HLA-DRB1 proved generally difficult to impute. Overall, <27.8% of subjects were correctly imputed for all HLA loci by any method...
April 25, 2017: Pharmacogenomics Journal
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