keyword
MENU ▼
Read by QxMD icon Read
search

Myosin C

keyword
https://www.readbyqxmd.com/read/28229860/distinct-contributions-of-the-thin-and-thick-filaments-to-length-dependent-activation-in-heart-muscle
#1
Xuemeng Zhang, Thomas Kampourakis, Ziqian Yan, Ivanka Sevrieva, Malcolm Irving, Yin-Biao Sun
The Frank-Starling relation is a fundamental auto-regulatory property of the heart that ensures the volume of blood ejected in each heartbeat is matched to the extent of venous filling. At the cellular level, heart muscle cells generate higher force when stretched, but despite intense efforts the underlying molecular mechanism remains unknown. We applied a fluorescence-based method, which reports structural changes separately in the thick and thin filaments of rat cardiac muscle, to elucidate that mechanism...
February 23, 2017: ELife
https://www.readbyqxmd.com/read/28223172/a-simple-model-of-cardiac-muscle-for-multiscale-simulation-passive-mechanics-crossbridge-kinetics-and-calcium-regulation
#2
Fyodor A Syomin, Andrey K Tsaturyan
A simple model of cardiac muscle was designed for multiscale simulation of heart mechanics. Relaxed cardiac muscle was described as a transversally isotropic hyperelastic material. Active tension caused by actin-myosin crossbridges depends on the ensemble averaged strain of myosin heads bound to actin. Calcium activation was modeled by Ca(2+) binding to troponin-C. To account for the dependence of troponin affinity for Ca(2+) on myosin heads strongly bound to actin, the kinetics of troponin binding to Ca(2+) in the overlap zone of the thin and thick filaments and outside it were separated...
February 18, 2017: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/28223068/parasite-control-and-skeletal-myositis-in-trypanosoma-cruzi-infected-and-exercised-rats
#3
Rômulo D Novaes, Reggiani V Gonçalves, Arlete R Penitente, Marli C Cupertino, Izabel R S C Maldonado, André Talvani, Antônio J Natali
Non-pharmacological strategies have been rarely described in the treatment of infectious diseases. Although exercise training has been recently incorporated in the clinical management of Chagas disease, the rationale basis that supports this indication is poorly understood. Thus, we investigated the effect of an aerobic exercise on the parasitism, inflammation and oxidative tissue damage in a murine model of Trypanosoma cruzi-induced skeletal myositis. Wistar rats were randomized into four groups: trained not infected (TNI) and infected (TI), sedentary not infected (SNI) and infected (SI)...
February 18, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28221712/discovery-of-myh14-as-an-important-and-unique-deafness-gene-causing-prelingually-severe-autosomal-dominant-non-syndromic-hearing-loss
#4
Bong Jik Kim, Ah Reum Kim, Jin Hee Han, Chung Lee, Doo Yi Oh, Byung Yoon Choi
BACKGROUND: Pathogenic variants of MYH14 have been known to be associated-in either a syndromic or non-syndromic manner-with hearing loss. Interestingly, all reported cases to date of MYH14-related non-syndromic hearing loss with detailed phenotypes have demonstrated mild-to-moderate progressive hearing loss with postlingual onset. METHODS: In this study, targeted resequencing (TRS) of known deafness genes was performed to identify the causative variant in two multiplex families segregating AD inherited hearing loss...
February 21, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/28212799/rho-mancing-to-sensitize-calcium-signaling-for-contraction-in-the-vasculature-role-of-rho-kinase
#5
T Szasz, R C Webb
Vascular smooth muscle contraction is an important physiological process contributing to cardiovascular homeostasis. The principal determinant of smooth muscle contraction is the intracellular free Ca(2+) concentration, and phosphorylation of myosin light chain (MLC) by activated myosin light chain kinase (MLCK) in response to increased Ca(2+) is the main pathway by which vasoconstrictor stimuli induce crossbridge cycling of myosin and actin filaments. A secondary pathway for vascular smooth muscle contraction that is not directly dependent on Ca(2+) concentration, but rather mediating Ca(2+) sensitization, is the RhoA/Rho kinase pathway...
2017: Advances in Pharmacology
https://www.readbyqxmd.com/read/28212798/protein-kinase-c-as-regulator-of-vascular-smooth-muscle-function-and-potential-target-in-vascular-disorders
#6
H C Ringvold, R A Khalil
Vascular smooth muscle (VSM) plays an important role in maintaining vascular tone. In addition to Ca(2+)-dependent myosin light chain (MLC) phosphorylation, protein kinase C (PKC) is a major regulator of VSM function. PKC is a family of conventional Ca(2+)-dependent α, β, and γ, novel Ca(2+)-independent δ, ɛ, θ, and η, and atypical ξ, and ι/λ isoforms. Inactive PKC is mainly cytosolic, and upon activation it undergoes phosphorylation, maturation, and translocation to the surface membrane, the nucleus, endoplasmic reticulum, and other cell organelles; a process facilitated by scaffold proteins such as RACKs...
2017: Advances in Pharmacology
https://www.readbyqxmd.com/read/28208809/a-lipid-emulsion-reverses-toxic-dose-bupivacaine-induced-vasodilation-during-tyrosine-phosphorylation-evoked-contraction-in-isolated-rat-aortae
#7
Seong-Ho Ok, Soo Hee Lee, Seong-Chun Kwon, Mun Hwan Choi, Il-Woo Shin, Sebin Kang, Miyeong Park, Jeong-Min Hong, Ju-Tae Sohn
The goal of this in vitro study was to examine the effect of a lipid emulsion on toxic-dose bupivacaine-induced vasodilation in a model of tyrosine phosphatase inhibitor sodium orthovanadate-induced contraction in endothelium-denuded rat aortae and to elucidate the associated cellular mechanism. The effect of a lipid emulsion on vasodilation induced by a toxic dose of a local anesthetic during sodium orthovanadate-induced contraction was examined. In addition, the effects of various inhibitors, either bupivacaine alone or a lipid emulsion plus bupivacaine, on protein kinase phosphorylation induced by sodium orthovanadate in rat aortic vascular smooth muscle cells was examined...
February 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28199848/discoidin-domain-receptor-1-mediates-myosin-dependent-collagen-contraction
#8
Nuno M Coelho, Pamma D Arora, Sander van Putten, Stellar Boo, Petar Petrovic, Alyna Xue Lin, Boris Hinz, Christopher A McCulloch
Discoidin domain receptor 1 (DDR1) is a tyrosine kinase collagen adhesion receptor that mediates cell migration through association with non-muscle myosin IIA (NMIIA). Because DDR1 is implicated in cancer fibrosis, we hypothesized that DDR1 interacts with NMIIA to enable collagen compaction by traction forces. Mechanical splinting of rat dermal wounds increased DDR1 expression and collagen alignment. In periodontal ligament of DDR1 knockout mice, collagen mechanical reorganization was reduced >30%. Similarly, cultured cells with DDR1 knockdown or expressing kinase-deficient DDR1d showed 50% reduction of aligned collagen...
February 14, 2017: Cell Reports
https://www.readbyqxmd.com/read/28194011/self-organizing-actin-patterns-shape-membrane-architecture-but-not-cell-mechanics
#9
M Fritzsche, D Li, H Colin-York, V T Chang, E Moeendarbary, J H Felce, E Sezgin, G Charras, E Betzig, C Eggeling
Cell-free studies have demonstrated how collective action of actin-associated proteins can organize actin filaments into dynamic patterns, such as vortices, asters and stars. Using complementary microscopic techniques, we here show evidence of such self-organization of the actin cortex in living HeLa cells. During cell adhesion, an active multistage process naturally leads to pattern transitions from actin vortices over stars into asters. This process is primarily driven by Arp2/3 complex nucleation, but not by myosin motors, which is in contrast to what has been theoretically predicted and observed in vitro...
February 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28193612/lack-of-phenotypic-differences-by-cardiovascular-magnetic-resonance-imaging-in-myh7-%C3%AE-myosin-heavy-chain-versus-mybpc3-myosin-binding-protein-c-related-hypertrophic-cardiomyopathy
#10
Adaya Weissler-Snir, Waseem Hindieh, Christiane Gruner, Dana Fourey, Evan Appelbaum, Ethan Rowin, Melanie Care, John R Lesser, Tammy S Haas, James E Udelson, Warren J Manning, Iacopo Olivotto, Benedetta Tomberli, Barry J Maron, Martin S Maron, Andrew M Crean, Harry Rakowski, Raymond H Chan
BACKGROUND: The 2 most commonly affected genes in hypertrophic cardiomyopathy (HCM) are MYH7 (β-myosin heavy chain) and MYBPC3 (β-myosin-binding protein C). Phenotypic differences between patients with mutations in these 2 genes have been inconsistent. Scarce data exist on the genotype-phenotype association as assessed by tomographic imaging using cardiac magnetic resonance imaging. METHODS AND RESULTS: Cardiac magnetic resonance imaging was performed on 358 consecutive genotyped hypertrophic cardiomyopathy probands at 5 tertiary hypertrophic cardiomyopathy centers...
February 2017: Circulation. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28181905/the-interplay-of-stiffness-and-force-anisotropies-drive-embryo-elongation
#11
Thanh Thi Kim Vuong-Brender, Martine Ben Amar, Julien Pontabry, Michel Labouesse
The morphogenesis of tissues, like the deformation of an object, results from the interplay between their material properties and the mechanical forces exerted on them. Whereas the importance of mechanical forces in influencing cell behaviour is widely recognized, the importance of tissue material properties, in particular stiffness, has received much less attention. Using C. elegans as a model, we examine how both aspects contribute to embryonic elongation. Measuring the opening shape of the epidermal actin cortex after laser nano-ablation, we assess the spatiotemporal changes of actomyosin-dependent force and stiffness along the antero-posterior and dorso-ventral axis...
February 9, 2017: ELife
https://www.readbyqxmd.com/read/28181366/molecular-phenotype-and-bleeding-risks-of-an-inherited-platelet-disorder-in-a-family-with-a-runx1-frameshift-mutation
#12
M S Badin, J K Iyer, M Chong, L Graf, G E Rivard, J S Waye, A D Paterson, G Pare, C P M Hayward
INTRODUCTION: Inherited defects in RUNX1 are important causes of platelet function disorders. AIM: Our goals were to evaluate RUNX1-related platelet disorders among individuals evaluated for uncharacterized, inherited platelet function disorders and test a proof of concept that bleeding risks could be quantitatively estimated for typical families with an inherited platelet function disorder. METHODS: Index cases with an uncharacterized inherited platelet function disorder were subjected to exome sequencing with confirmation of RUNX1 mutations by Sanger sequencing...
February 8, 2017: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/28178619/effect-of-qishenyiqi-pill-on-myocardial-collagen-metabolism-in-experimental-autoimmune-myocarditis-rats
#13
Shi-Chao Lv, Meifang Wu, Meng Li, Qiang Wang, Xiao-Jing Wang, Ao Zhang, Ling Xu, Jun-Ping Zhang
OBJECTIVE: To observe the effect of QiShenYiQi pill (QSYQ) on myocardial collagen metabolism in experimental autoimmune myocarditis rats, and to explore its mechanism of action. METHODS: Lewis rats underwent the injection of myocardial myosin mixed with freund's complete adjuvant were randomized into three groups: model, valsartan and QSYQ groups. And we treated rats which were injected phosphate buffered saline (PBS) mixed with freund's complete adjuvant as control group...
February 5, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28167762/phosphorylation-of-cardiac-myosin-binding-protein-c-releases-myosin-heads-from-the-surface-of-cardiac-thick-filaments
#14
Robert W Kensler, Roger Craig, Richard L Moss
Cardiac myosin binding protein C (cMyBP-C) has a key regulatory role in cardiac contraction, but the mechanism by which changes in phosphorylation of cMyBP-C accelerate cross-bridge kinetics remains unknown. In this study, we isolated thick filaments from the hearts of mice in which the three serine residues (Ser273, Ser282, and Ser302) that are phosphorylated by protein kinase A in the m-domain of cMyBP-C were replaced by either alanine or aspartic acid, mimicking the fully nonphosphorylated and the fully phosphorylated state of cMyBP-C, respectively...
February 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28158907/naturally-occurring-biventricular-noncompaction-in-an-adult-domestic-cat
#15
M D Kittleson, P R Fox, C Basso, G Thiene
A definitively diagnosed case of left ventricular noncompaction (LVNC) has not been previously reported in a non-human species. We describe a Maine Coon cross cat with echocardiographically and pathologically documented LVNC. The cat was from a research colony and was heterozygous for the cardiac myosin binding protein C mutation associated with hypertrophic cardiomyopathy (HCM) in Maine Coon cats (A31P). The cat had had echocardiographic examinations performed every 6 months until 6 years of age at which time the cat died of an unrelated cause...
February 3, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28153611/phosphoglycerol-dihydroceramide-a-distinctive-ceramide-produced-by-porphyromonas-gingivalis-promotes-rankl-induced-osteoclastogenesis-by-acting-on-non-muscle-myosin-ii-a-myh9-an-osteoclast-cell-fusion-regulatory-factor
#16
Hiroyuki Kanzaki, Alexandru Movila, Rayyan Kayal, Marcelo H Napimoga, Kenji Egashira, Floyd Dewhirst, Hajime Sasaki, Mohammed Howait, Ayman Al-Dharrab, Abdulghani Mira, Xiaozhe Han, Martin A Taubman, Frank C Nichols, Toshihisa Kawai
Among several virulence factors produced by the periodontal pathogen Porphyromonas gingivalis (Pg), a recently identified novel class of dihydroceramide lipids that contains a long acyl-chain has the potential to play a pathogenic role in periodontitis because of its higher level of tissue penetration compared to other lipid classes produced by Pg. However, the possible impact of Pg ceramides on osteoclastogenesis is largely unknown. In the present study, we report that the phosphoglycerol dihydroceramide (PGDHC) isolated from Pg enhanced osteoclastogenesis in vitro and in vivo...
January 31, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28148779/adverse-left-ventricular-remodeling-by-glycoprotein-nonmetastatic-melanoma-protein-b-in-myocardial-infarction
#17
Anne Järve, Silke Mühlstedt, Fatimunnisa Qadri, Bernadette Nickl, Herbert Schulz, Norbert Hübner, Cemil Özcelik, Michael Bader
Cardiac diseases are the leading cause of death. Available treatment approaches are not sufficient to reverse persistent cardiac damage after injury; thus, the search for new therapeutic targets is essential. Our microarray-based screening in rat hearts 24 h after myocardial infarction (MI) yielded glycoprotein nonmetastatic melanoma protein B (GPNMB), which is known to be involved in inflammation and fibrosis after tissue injury. However, its role in the heart was elusive. We found increased cardiac expression levels of GPNMB in rats and mice after MI...
February 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28143933/filopodia-formation-and-endosome-clustering-induced-by-mutant-plus-end-directed-myosin-vi
#18
Thomas A Masters, Folma Buss
Myosin VI (MYO6) is the only myosin known to move toward the minus end of actin filaments. It has roles in numerous cellular processes, including maintenance of stereocilia structure, endocytosis, and autophagosome maturation. However, the functional necessity of minus-end-directed movement along actin is unclear as the underlying architecture of the local actin network is often unknown. To address this question, we engineered a mutant of MYO6, MYO6+, which undergoes plus-end-directed movement while retaining physiological cargo interactions in the tail...
January 31, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28130336/peroxisome-proliferator-activated-receptor-%C3%AE-expression-induces-alterations-in-cardiac-myofilaments-in-a-pressure-overload-model-of-hypertrophy
#19
Chehade N Karam, Chad M Warren, Marcus Henze, Natasha Hausler Banke, E Douglas Lewandowski, R John Solaro
Although alterations in fatty acid (FA) metabolism have been shown to have a negative impact on contractility of the hypertrophied heart, the targets of action remain elusive. In this study we compared the function of skinned fiber bundles from transgenic (Tg) mice, that over express a relatively low level of the peroxisome proliferator-activated receptor α (PPARα), and non-transgenic (NTg) littermates. The mice (NTg-T and Tg-T) were stressed by transverse aortic constriction (TAC) and compared to shams (NTg-S and Tg-S)...
January 27, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28130125/histone-methyltransferase-setd2-is-critical-for-the-proliferation-and-differentiation-of-myoblasts
#20
Xin Yi, Ye Tao, Xi Lin, Yuan Dai, Tingli Yang, Xiaojing Yue, Xuejun Jiang, Xiaoyan Li, Ding-Sheng Jiang, Kelsey C Andrade, Jiang Chang
Skeletal muscle cell proliferation and differentiation are tightly regulated. Epigenetic regulation is a major component of the regulatory mechanism governing these processes. Histone modification is part of the epigenetic code used for transcriptional regulation of chromatin through the establishment of an active or repressive state for genes involved in myogenesis in a temporal manner. Here, we uncovered the function of SET domain containing 2 (Setd2), an essential histone 3 lysine 36 trimethyltransferase, in regulating the proliferation and differentiation of myoblasts...
January 24, 2017: Biochimica et Biophysica Acta
keyword
keyword
104239
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"