Megan J Myers, Zhen Xu, Benjamin J Ryan, Zachary R DeMars, Miranda J Ridder, David K Johnson, Christina N Krute, Tony S Flynn, Maithri M Kashipathy, Kevin P Battaile, Nicholas Schnicker, Scott Lovell, Bret D Freudenthal, Jeffrey L Bose
Gram-positive bacteria utilize a Fatty Acid Kinase (FAK) complex to harvest fatty acids from the environment. The complex, consisting of the fatty acid kinase, FakA, and an acyl carrier protein, FakB, is known to impact virulence and disease outcomes. However, FAK's structure and enzymatic mechanism remain poorly understood. Here, we used a combination of modeling, biochemical, and cell-based approaches to establish critical details of FAK activity. Solved structures of the apo and ligand-bound FakA kinase domain captured the protein state through ATP hydrolysis...
March 19, 2024: bioRxiv