keyword
MENU ▼
Read by QxMD icon Read
search

phage shock protein

keyword
https://www.readbyqxmd.com/read/29018419/gene-expression-response-of-salmonella-enterica-serotype-enteritidis-phage-type-8-to-subinhibitory-concentrations-of-the-plant-derived-compounds-trans-cinnamaldehyde-and-eugenol
#1
Anup Kollanoor Johny, Jonathan G Frye, Annie Donoghue, Dan J Donoghue, Steffen Porwollik, Michael McClelland, Kumar Venkitanarayanan
Background:Salmonella Enteritidis phage type 8 (PT8) is a major poultry-associated Salmonella strain implicated in foodborne outbreaks in the United States. We previously reported that two plant-derived compounds generally recognized as safe (GRAS), trans-cinnamaldehyde (TC), and eugenol (EG), significantly reduced S. Enteritidis colonization in broiler and layer chickens. To elucidate potential PT8 genes affected by TC and EG during colonization, a whole-genome microarray analysis of the bacterium treated with TC and EG was conducted...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28979244/lactobacillus-casei-bl23-produces-microvesicles-carrying-proteins-that-have-been-associated-with-its-probiotic-effect
#2
A Paula Domínguez Rubio, Jimena H Martínez, Diana C Martínez Casillas, Federico Coluccio Leskow, Mariana Piuri, Oscar E Pérez
Archaea, bacteria, and eukarya secrete membrane microvesicles (MVs) as a mechanism for intercellular communication. We report the isolation and characterization of MVs from the probiotic strain Lactobacillus casei BL23. MVs were characterized using analytical high performance techniques, DLS, AFM and TEM. Similar to what has been described for other Gram-positive bacteria, MVs were on the nanometric size range (30-50 nm). MVs carried cytoplasmic components such as DNA, RNA and proteins. Using a proteomic approach (LC-MS), we identified a total of 103 proteins; 13 exclusively present in the MVs...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28922502/the-three-component-system-esrisr-regulates-a-cell-envelope-stress-response-in-corynebacterium-glutamicum
#3
Britta Kleine, Ava Chattopadhyay, Tino Polen, Daniela Pinto, Thorsten Mascher, Michael Bott, Melanie Brocker, Roland Freudl
When the cell envelope integrity is compromised, bacteria trigger signaling cascades resulting in the production of proteins that counteract these extracytoplasmic stresses. Here, we show that the two-component system EsrSR regulates a cell envelope stress response in the Actinobacterium Corynebacterium glutamicum. The sensor kinase EsrS possesses an amino-terminal phage shock protein C (PspC) domain, a property that sets EsrSR apart from all other two-component systems characterized so far. An integral membrane protein, EsrI, whose gene is divergently transcribed to the esrSR gene locus and which interestingly also possesses a PspC domain, acts as an inhibitor of EsrSR under non-stress conditions...
September 18, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28900025/psp-stress-response-proteins-form-a-complex-with-mislocalized-secretins-in-the-yersinia-enterocolitica-cytoplasmic-membrane
#4
Disha Srivastava, Amal Moumene, Josué Flores-Kim, Andrew J Darwin
The bacterial phage shock protein system (Psp) is a conserved extracytoplasmic stress response that is essential for the virulence of some pathogens, including Yersinia enterocolitica It is induced by events that can compromise inner membrane (IM) integrity, including the mislocalization of outer membrane pore-forming proteins called secretins. In the absence of the Psp system, secretin mislocalization permeabilizes the IM and causes rapid cell death. The Psp proteins PspB and PspC form an integral IM complex with two independent roles...
September 12, 2017: MBio
https://www.readbyqxmd.com/read/28588134/the-rcs-regulated-colanic-acid-capsule-maintains-membrane-potential-in-salmonella-enterica-serovar-typhimurium
#5
Jasmine M Pando, Joyce E Karlinsey, Jimmie C Lara, Stephen J Libby, Ferric C Fang
The Rcs phosphorelay and Psp (phage shock protein) systems are envelope stress responses that are highly conserved in gammaproteobacteria. The Rcs regulon was found to be strongly induced during metal deprivation of Salmonella enterica serovar Typhimurium lacking the Psp response. Nineteen genes activated by the RcsA-RcsB response regulator make up an operon responsible for the production of colanic acid capsular polysaccharide, which promotes biofilm development. Despite more than half a century of research, the physiological function of colanic acid has remained elusive...
June 6, 2017: MBio
https://www.readbyqxmd.com/read/28551403/chaperone-like-activity-of-synthetic-polyanions-can-be-higher-than-the-activity-of-natural-chaperones-at-elevated-temperature
#6
Pavel I Semenyuk, Lidia P Kurochkina, Nikolai B Gusev, Vladimir A Izumrudov, Vladimir I Muronetz
Polyelectrolytes are a prospective tool for protection of proteins against aggregation. We compared synthetic polyanion, poly(styrene sulfonate), and natural chaperones of different types, namely, GroEL-like chaperonin from Pseudomonas aeruginosa phage EL and human small heat shock protein HspB5 (αB-crystallin), in their ability to prevent aggregation of client proteins. At 45 °C, all three agents efficiently suppressed thermal aggregation of phage endolysin. At higher temperatures, HspB5 and poly(styrene sulfonate) also inhibited endolysin aggregation, though polyanion became less efficient than HspB5 at 55 °C and 60 °C...
July 22, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28505134/a-novel-roseosiphophage-isolated-from-the-oligotrophic-south-china-sea
#7
Yunlan Yang, Lanlan Cai, Ruijie Ma, Yongle Xu, Yigang Tong, Yong Huang, Nianzhi Jiao, Rui Zhang
The Roseobacter clade is abundant and widespread in marine environments and plays an important role in oceanic biogeochemical cycling. In this present study, a lytic siphophage (labeled vB_DshS-R5C) infecting the strain type of Dinoroseobacter shibae named DFL12(T), which is part of the Roseobacter clade, was isolated from the oligotrophic South China Sea. Phage R5C showed a narrow host range, short latent period and low burst size. The genome length of phage R5C was 77, 874 bp with a G+C content of 61.5%. Genomic comparisons detected no genome matches in the GenBank database and phylogenetic analysis based on DNA polymerase I revealed phylogenetic features that were distinct to other phages, suggesting the novelty of R5C...
May 15, 2017: Viruses
https://www.readbyqxmd.com/read/27923541/protecting-from-envelope-stress-variations-on-the-phage-shock-protein-theme-trends-in-microbiology-25-first-page-en-dash-last-page-2017
#8
Riccardo Manganelli, Maria Laura Gennaro
No abstract text is available yet for this article.
December 3, 2016: Trends in Microbiology
https://www.readbyqxmd.com/read/27879679/hydrostatin-tl1-an-anti-inflammatory-active-peptide-from-the-venom-gland-of-hydrophis-cyanocinctus-in-the-south-china-sea
#9
Ningyuan Wang, Yan Huang, An Li, Hailong Jiang, Jie Wang, Jianzhong Li, Lei Qiu, Ka Li, Yiming Lu
Tumor necrosis factor (TNF)-α is a pleiotropic cytokine with intense pro-inflammatory and immunomodulatory properties, and anti-TNF-α biologics are effective therapies for various inflammatory diseases such as inflammatory bowel disease (IBD) and sepsis. Snake venom, as a traditional Chinese medicine, has been used in the treatment of inflammatory diseases in China for centuries. In this research, we constructed a venom gland T7 phage display library of the sea snake Hydrophis cyanocinctus to screen bioactive compounds that antagonize TNF-α and identified a novel nine-amino-acid peptide, termed hydrostatin-TL1 (H-TL1)...
November 22, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27865622/protecting-from-envelope-stress-variations-on-the-phage-shock-protein-theme
#10
REVIEW
Riccardo Manganelli, Maria Laura Gennaro
During envelope stress, critical inner-membrane functions are preserved by the phage-shock-protein (Psp) system, a stress response that emerged from work with Escherichia coli and other Gram-negative bacteria. Reciprocal regulatory interactions and multiple effector functions are well documented in these organisms. Searches for the Psp system across phyla reveal conservation of only one protein, PspA. However, examination of Firmicutes and Actinobacteria reveals that PspA orthologs associate with non-orthologous regulatory and effector proteins retaining functions similar to those in Gram-negative counterparts...
March 2017: Trends in Microbiology
https://www.readbyqxmd.com/read/27806910/structure-and-function-of-pspa-and-vipp1-n-terminal-peptides-insights-into-the-membrane-stress-sensing-and-mitigation
#11
Christopher McDonald, Goran Jovanovic, B A Wallace, Oscar Ces, Martin Buck
The phage shock protein (Psp) response maintains integrity of the inner membrane (IM) in response to extracytoplasmic stress conditions and is widely distributed amongst enterobacteria. Its central component PspA, a member of the IM30 peripheral membrane protein family, acts as a major effector of the system through its direct association with the IM. Under non-stress conditions PspA also negatively regulates its own expression via direct interaction with the AAA+ ATPase PspF. PspA has a counterpart in cyanobacteria called Vipp1, which is implicated in protection of the thylakoid membranes...
January 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27698088/interactions-between-the-cytoplasmic-domains-of-pspb-and-pspc-silence-the-yersinia-enterocolitica-phage-shock-protein-response
#12
Josué Flores-Kim, Andrew J Darwin
The phage shock protein (Psp) system is a widely conserved cell envelope stress response that is essential for the virulence of some bacteria, including Yersinia enterocolitica Recruitment of PspA by the inner membrane PspB-PspC complex characterizes the activated state of this response. The PspB-PspC complex has been proposed to be a stress-responsive switch, changing from an OFF to an ON state in response to an inducing stimulus. In the OFF state, PspA cannot access its binding site in the C-terminal cytoplasmic domain of PspC (PspC(CT)), because this site is bound to PspB...
December 15, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27678123/comparative-genome-analysis-of-the-daptomycin-resistant-streptococcus-anginosus-strain-j4206-associated-with-breakthrough-bacteremia
#13
Maliha Rahman, Scott V Nguyen, Kimberly A McCullor, Catherine J King, James H Jorgensen, W Michael McShan
Streptococcus anginosus is a member of the normal oral flora that can become a pathogen causing pyogenic infections in humans. The genome of daptomycin-resistant strain J4206, originally isolated from a patient suffering from breakthrough bacteremia and septic shock at the University of Texas Health Science Center at San Antonio, was determined. The circular genome is 2,001,352 bp long with a GC content of 38.62% and contains multiple mobile genetic elements, including the phage-like chromosomal island SanCI that mediates a mutator phenotype, transposons, and integrative conjugative elements...
December 31, 2016: Genome Biology and Evolution
https://www.readbyqxmd.com/read/27659529/induction-of-anti-egfr-immune-response-with-mimotopes-identified-from-a-phage-display-peptide-library-by-panitumumab
#14
Aidong Wang, Ming Cui, Hong Qu, Jiabo Di, Zaozao Wang, Jiadi Xing, Fan Wu, Wei Wu, Xicheng Wang, Lin Shen, Beihai Jiang, Xiangqian Su
The epidermal growth factor receptor (EGFR) is overexpressed in several epithelial tumors. Anti-EGFR humanized monoclonal antibodies, cetuximab and panitumumab, in combination with chemotherapy have improved the prognosis for patients with wild-type RAS tumors. To identify mimotopes of EGFR and develop mimotope-based EGFR vaccines, we screened a phage display peptide library with panitumumab. Two EGFR mimotopes P19 and P26, which could be recognized by panitumumab specifically, were isolated. To enhance the immune responses, we generated recombinant proteins of P19 or P26 fused to a heat-shock cognate protein 70 (Hsc70), and evaluated the efficacy of Hsc70-P19 and Hsc70-P26 as vaccines in vivo...
November 15, 2016: Oncotarget
https://www.readbyqxmd.com/read/27643500/in-vitro-structural-and-functional-characterization-of-the-small-heat-shock-proteins-shsp-of-the-cyanophage-s-shm2-and-its-host-synechococcus-sp-wh7803
#15
Maxime Bourrelle-Langlois, Geneviève Morrow, Stéphanie Finet, Robert M Tanguay
We previously reported the in silico characterization of Synechococcus sp. phage 18 kDa small heat shock protein (HspSP-ShM2). This small heat shock protein (sHSP) contains a highly conserved core alpha crystalline domain of 92 amino acids and relatively short N- and C-terminal arms, the later containing the classical C-terminal anchoring module motif (L-X-I/L/V). Here we establish the oligomeric profile of HspSP-ShM2 and its structural dynamics under in vitro experimental conditions using size exclusion chromatography (SEC/FPLC), gradient native gels electrophoresis and dynamic light scattering (DLS)...
2016: PloS One
https://www.readbyqxmd.com/read/27583250/modulating-salmonella-typhimurium-s-response-to-a-changing-environment-through-bacterial-enhancer-binding-proteins-and-the-rpon-regulon
#16
REVIEW
Christine E Hartman, David J Samuels, Anna C Karls
Transcription sigma factors direct the selective binding of RNA polymerase holoenzyme (Eσ) to specific promoters. Two families of sigma factors determine promoter specificity, the σ(70) (RpoD) family and the σ(54) (RpoN) family. In transcription controlled by σ(54), the Eσ(54)-promoter closed complex requires ATP hydrolysis by an associated bacterial enhancer-binding protein (bEBP) for the transition to open complex and transcription initiation. Given the wide host range of Salmonella enterica serovar Typhimurium, it is an excellent model system for investigating the roles of RpoN and its bEBPs in modulating the lifestyle of bacteria...
2016: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/27297125/the-phage-shock-protein-response
#17
REVIEW
Josué Flores-Kim, Andrew J Darwin
The phage shock protein (Psp) system was identified as a response to phage infection in Escherichia coli, but rather than being a specific response to a phage, it detects and mitigates various problems that could increase inner-membrane (IM) permeability. Interest in the Psp system has increased significantly in recent years due to appreciation that Psp-like proteins are found in all three domains of life and because the bacterial Psp response has been linked to virulence and other important phenotypes. In this article, we summarize our current understanding of what the Psp system detects and how it detects it, how four core Psp proteins form a signal transduction cascade between the IM and the cytoplasm, and current ideas that explain how the Psp response keeps bacterial cells alive...
September 8, 2016: Annual Review of Microbiology
https://www.readbyqxmd.com/read/27193545/transcription-regulation-and-membrane-stress-management-in-enterobacterial-pathogens
#18
REVIEW
Nan Zhang, Goran Jovanovic, Christopher McDonald, Oscar Ces, Xiaodong Zhang, Martin Buck
Transcription regulation in a temporal and conditional manner underpins the lifecycle of enterobacterial pathogens. Upon exposure to a wide array of environmental cues, these pathogens modulate their gene expression via the RNA polymerase and associated sigma factors. Different sigma factors, either involved in general 'house-keeping' or specific responses, guide the RNA polymerase to their cognate promoter DNAs. The major alternative sigma54 factor when activated helps pathogens manage stresses and proliferate in their ecological niches...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27034429/a-transplant-immunome-screening-platform-defines-a-targetable-epitope-fingerprint-of-multiple-myeloma
#19
Aneta Schieferdecker, Anna Oberle, Benjamin Thiele, Fabian Hofmann, Markus Göthel, Sebastian Miethe, Michael Hust, Friederike Braig, Mareike Voigt, Ute-Marie von Pein, Friedrich Koch-Nolte, Friedrich Haag, Malik Alawi, Daniela Indenbirken, Adam Grundhoff, Carsten Bokemeyer, Ulrike Bacher, Nicolaus Kröger, Mascha Binder
Multiple myeloma (MM) is a hematological cancer for which immune-based treatments are currently in development. Many of these rely on the identification of highly disease-specific, strongly and stably expressed antigens. Here, we profiled the myeloma B-cell immunome both to explore its predictive role in the context of autologous and allogeneic hematopoietic stem cell transplantation (HSCT) and to identify novel immunotherapeutic targets. We used random peptide phage display, reverse immunization, and next-generation sequencing-assisted antibody phage display to establish a highly myeloma-specific epitope fingerprint targeted by B-cell responses of 18 patients in clinical remission...
June 23, 2016: Blood
https://www.readbyqxmd.com/read/27002134/rv2744c-is-a-pspa-ortholog-that-regulates-lipid-droplet-homeostasis-and-nonreplicating-persistence-in-mycobacterium-tuberculosis
#20
Richard M Armstrong, Katherine L Adams, Joseph E Zilisch, Daniel J Bretl, Hiromi Sato, David M Anderson, Thomas C Zahrt
UNLABELLED: Mycobacterium tuberculosis, the causative agent of tuberculosis (TB), remains a significant cause of morbidity and mortality worldwide, despite the availability of a live attenuated vaccine and anti-TB antibiotics. The vast majority of individuals infected with M. tuberculosis develop an asymptomatic latent infection in which the bacterium survives within host-generated granulomatous lesions in a physiologically altered metabolic state of nonreplicating persistence. The granuloma represents an adverse environment, as M...
June 1, 2016: Journal of Bacteriology
keyword
keyword
104211
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"