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diagnostic methods tuberculosis

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https://www.readbyqxmd.com/read/28088186/concentration-of-fine-needle-aspirates-similar-to-molecular-method-improves-sensitivity-of-the-diagnosis-of-tuberculous-lymphadenitis-in-addis-ababa-ethiopia
#1
Olifan Zewdie, Tamrat Abebe, Adane Mihret, Eyob Hirpa, Gobena Ameni
BACKGROUND: Tuberculous lymphadenitis (TBLN) diagnosis has been a true challenge solely by clinical evidence in developing countries, due to limited the diagnostic facility on hand. However, the availability and affordability of available diagnostic tools in resource-limited settings like Ethiopia necessitates the quest for other techniques with added value over direct Z-N microscopy. Therefore, we aimed at to assess whether the concentration of lymph node aspirate similarly improves the detection rate of tuberculous lymphadenitis or not...
January 14, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28086955/evaluation-of-the-xpert%C3%A2-mtb-rif-assay-and-microscopy-for-the-diagnosis-of-mycobacterium-tuberculosis-in-namibia
#2
Rooyen T Mavenyengwa, Emma Shaduka, Innocent Maposa
BACKGROUND: Tuberculosis (TB) kills approximately two million people and infects around nine million worldwide annually. Its proper management, especially in resource-limited settings, has been hindered by the lack of rapid and easy-to-use diagnostic tests. Sputum smear microscopy remains the cheapest, readily available diagnostic method but it only identifies less than half of the patients with a HIV/TB co-infection because the bacilli would have disseminated from the lungs to other areas of the body...
January 11, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28086877/treating-the-invisible-gaps-and-opportunities-for-enhanced-tb-control-along-the-thailand-myanmar-border
#3
Naomi Tschirhart, Sein Sein Thi, Lei Lei Swe, Francois Nosten, Angel M Foster
BACKGROUND: In Thailand's northwestern Tak province, contextual conditions along the border with Myanmar pose difficulties for TB control among migrant populations. Incomplete surveillance data, migrant patient mobility, and loss to follow-up make it difficult to estimate the TB burden and implement effective TB control measures. This multi-methods study examined tuberculosis, tuberculosis and human immunodeficiency virus co-infection, and multidrug-resistant tuberculosis treatment accessibility for migrants and refugees in Tak province, health system response, and public health surveillance...
January 13, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28081227/diagnostic-accuracy-of-genexpert-mtb-rif-assay-in-comparison-to-conventional-drug-susceptibility-testing-method-for-the-diagnosis-of-multidrug-resistant-tuberculosis
#4
Pratikshya Pandey, Narayan Dutt Pant, Komal Raj Rijal, Bhawana Shrestha, Sirita Kattel, Megha Raj Banjara, Bhagwan Maharjan, Rajendra Kc
Xpert MTB/RIF assay is regarded as a great achievement of modern medicine for the rapid diagnosis of multidrug-resistant tuberculosis (MDR-TB). The main purpose of this study was to determine the performance of Xpert MTB/RIF assay compared to conventional drug susceptibility testing (DST) method for the diagnosis of MDR-TB. A comparative cross sectional study was carried out at German-Nepal Tuberculosis Project, Kathmandu, Nepal, from April 2014 to September 2014. A total of 88 culture positive clinical samples (83 pulmonary and 5 extra-pulmonary) received during the study period were analyzed for detection of multidrug-resistant tuberculosis by both GeneXpert MTB/RIF assay and conventional DST method...
2017: PloS One
https://www.readbyqxmd.com/read/28072845/tuberculosis-detection-in-paratuberculosis-vaccinated-calves-new-alternatives-against-interference
#5
Miriam Serrano, Natalia Elguezabal, Iker A Sevilla, María V Geijo, Elena Molina, Rakel Arrazuria, Alfonso Urkitza, Gareth J Jones, Martin Vordermeier, Joseba M Garrido, Ramón A Juste
Paratuberculosis vaccination in cattle has been restricted due to its possible interference with the official diagnostic methods used in tuberculosis eradication programs. To overcome this drawback, new possibilities to detect Mycobacterium bovis infected cattle in paratuberculosis vaccinated animals were studied under experimental conditions. Three groups of 5 calves each were included in the experiment: one paratuberculosis vaccinated group, one paratuberculosis vaccinated and M. bovis infected group and one M...
2017: PloS One
https://www.readbyqxmd.com/read/28070050/-clinical-comparative-analysis-for-pulmonary-histoplasmosis-and-progressive-disseminated-histoplasmosis
#6
Yan Zhang, Xiaoli Su, Yuanyuan Li, Ruoxi He, Chengping Hu, Pinhua Pan
To compare clinical features, diagnosis and therapeutic effect between pulmonary histoplasmosis and progressive disseminated histoplasmosis.
 Methods: A retrospective analysis for 12 cases of hospitalized patients with histoplasmosis, who was admitted in Xiangya Hospital, Central South University during the time from February 2009 to October 2015, was carried out. Four cases of pulmonary histoplasmosis and 8 cases of progressive disseminated histoplasmosis were included. The differences of clinical features, imaging tests, means for diagnosis and prognosis were analyzed between the two types of histoplasmosis...
December 28, 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28068347/microbiologically-confirmed-tuberculosis-factors-associated-with-pre-treatment-loss-to-follow-up-and-time-to-treatment-initiation
#7
Judith Mwansa-Kambafwile, Boitumelo Maitshotlo, Andrew Black
BACKGROUND: The impact of new diagnostics on pre-treatment loss to follow up (Pre-treatment LTFU) has not been widely investigated. The reported rate of pre-treatment LTFU is however lower in studies where Xpert MTB/Rif (Xpert) has been used onsite as opposed to centrally. The use of the Xpert at point of care (POC) could have a role in reducing the pre-treatment LTFU rate among TB patients. We aimed to determine the pre-treatment LTFU rate and the time to treatment initiation as well as to describe associated factors in patients diagnosed with TB using POC Xpert or smear microscopy...
2017: PloS One
https://www.readbyqxmd.com/read/28065665/unbiased-identification-of-blood-based-biomarkers-for-pulmonary-tuberculosis-by-modeling-and-mining-molecular-interaction-networks
#8
Awanti Sambarey, Abhinandan Devaprasad, Abhilash Mohan, Asma Ahmed, Soumya Nayak, Soumya Swaminathan, George D'Souza, Anto Jesuraj, Chirag Dhar, Subash Babu, Annapurna Vyakarnam, Nagasuma Chandra
Efficient diagnosis of tuberculosis (TB) is met with multiple challenges, calling for a shift of focus from pathogen-centric diagnostics towards identification of host-based multi-marker signatures. Transcriptomics offer a list of differentially expressed genes, but cannot by itself identify the most influential contributors to the disease phenotype. Here, we describe a computational pipeline that adopts an unbiased approach to identify a biomarker signature. Data from RNA sequencing from whole blood samples of TB patients were integrated with a curated genome-wide molecular interaction network, from which we obtain a comprehensive perspective of variations that occur in the host due to TB...
February 2017: EBioMedicine
https://www.readbyqxmd.com/read/28061885/diagnostic-yield-of-nontuberculous-mycobacteria-in-patients-booked-for-endoscopy-at-the-university-teaching-hospital-lusaka
#9
Gershom Chongwe, Charles Michelo, Paul Kelly
BACKGROUND: The intestinal carriage of nontuberculous mycobacteria (NTM) is associated with disease, especially in severely immunocompromised individuals. These organisms, although often considered contaminants, have been known to cause various types of illnesses. We aimed to determine the prevalence of and associated factors for NTM among patients booked for colonoscopy at the University Teaching Hospital (UTH) in Lusaka. METHODS: We randomly recruited 97 patients attending routine endoscopy procedures between November 2012 and October 2013 and after consent, administered a structured questionnaire...
January 7, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28057604/biological-markers-in-the-diagnosis-of-tuberculous-pleural-effusion
#10
Bastien Mollo, Stéphane Jouveshomme, François Philippart, Benoît Pilmis
Tuberculosis is one of the main etiologies to evoke in the context of lymphocyte pleurisy. However, diagnosis is difficult and is based on mycobacteriology that is not enough sensitive and time-consuming, or on histology that requires invasive biopsy gesture. This literature review, carried out from Medline, summarizes the main meta-analyzes, reviews, and originator publications in English on biomarkers, classic and more innovative, studied for the diagnosis of tuberculous pleurisy. Among the immuno-biochemical markers, interferon-γ (IFN-γ), isoenzyme of adenosine deaminase 2 (ADA2) and total adenosine deaminase (ADA) seem the most relevant with respective sensitivities of 89% (87-91), 97...
January 5, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28050691/yield-of-chest-x-ray-tuberculosis-screening-of-immigrants-during-the-european-refugee-crisis-of-2015-a-single-centre-experience
#11
Julius Matthias Weinrich, Roland Diel, Markus Sauer, Frank Oliver Henes, Karen Meywald-Walter, Gerhard Adam, Gerhard Schön, Peter Bannas
OBJECTIVE: Our aim was to determine the prevalence of tuberculosis (TB), the number needed to screen (NNS), and the diagnostic accuracy of chest X-ray (CXR) screening to detect active pulmonary TB during the 2015 European refugee crisis. MATERIALS AND METHODS: We evaluated data of all refugees who underwent CXR screening in a single-centre of one German metropolitan area in 2015. We determined the prevalence of TB, NNS, and accuracy of CXR to detect active pulmonary TB...
January 3, 2017: European Radiology
https://www.readbyqxmd.com/read/28043620/implementation-of-new-tools-for-multidrug-resistant-tuberculosis-detection-and-control
#12
Robin M Warren
Contrary to classical dogma, molecular epidemiological studies and mathematical modeling clearly show that the global drug-resistant tuberculosis (TB) epidemic is driven by transmission. Furthermore, there is mounting evidence demonstrating that amplification of resistance occurs during treatment, possibly as a result of insufficient active drugs being administered. To control the drug-resistant TB epidemic, it will be essential to implement new, rapid, and highly sensitive and specific diagnostic methods to decrease the diagnostic delay associated with culture-based testing...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043608/new-options-in-tuberculosis-care-visions-for-the-future-are-crucial-for-controlling-the-disease
#13
Malin Ridell
The current strategies for controlling tuberculosis (TB) are not sufficient. Improved prophylactic and diagnostic tools are imperative, being crucial for decreasing TB incidence and mortality and for preventing outbreaks. Furthermore, new and better drugs are badly needed, particularly considering the increase in cases with multidrug-resistant strains. The current TB vaccine-the Bacillus Calmette-Guérin vaccine-has a preventive impact on disseminated TB in children, but little effect on the most common form of TB, that is, lung TB in adults and young adults...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043600/benefits-and-challenges-of-molecular-diagnostics-for-childhood-tuberculosis
#14
Cristina Gutierrez
Expanding tuberculosis (TB)-diagnostic services, including access to rapid tests, is a World Health Organization (WHO) strategy to accelerate progress toward ending TB. Faster and more sensitive molecular tests capable of diagnosing TB and drug-resistant TB have the technical capacity to address limitations associated with smears and cultures by increasing accuracy and shortening turnaround times as compared with those of these conventional laboratory methods. Nucleic acid amplification assays used to detect and analyze Mycobacterium tuberculosis (MTB)-complex nucleic acids can be used directly on specimens from patients suspected of having TB...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043587/cloning-expression-and-purification-of-recombinant-protein-mpt-64-from-a-virulent-strain-of-mycobacterium-bovis-in-a-prokaryotic-system
#15
Maryam Mohammadi Tashakkori, Majid Tebianian, Mohammad Tabatabaei, Nader Mosavari
OBJECTIVE: Tuberculosis (TB) is a zoonotic infectious disease common to humans and animals that is caused by the rod-shaped acid-fast bacterium Mycobacterium bovis. Rapid and sensitive detection of TB is promoted by specific antigens. Virulent strains of the TB complex from M. bovis contain 16 regions of difference (RD) in their genome that encode important proteins, including major protein of Mycobacterium Tuberculosis 64 (MBT-64, which is a primary immune-stimulating antigen encoded by RD-2...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043576/immunohistochemical-findings-of-the-granulomatous-reaction-associated-with-tuberculosis
#16
Shirin Karimi, Masoud Shamaei, Mihan Pourabdollah, Makan Sadr, Mehrdad Karbasi, Arda Kiani, Moslem Bahadori
OBJECTIVE/BACKGROUND: The histological diagnosis of Mycobacterium tuberculosis (MTB) has long been a diagnostic challenge in the anatomical pathology field despite availability of different laboratory methods. Immunohistochemistry (IHC) could not only confirm granulomatous tissue involvement but also demonstrate MTB antigen immunolocalization. This study tries to clarify the details of IHC staining for MTB with pAbBCG. METHODS: A total of 50 patients undergoing simultaneous biopsy and tissue culture with positive tissue culture for MTB during 2005-2009 were selected from the MRC Department at Masih Daneshvari Hospital, Tehran, Iran...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043570/evaluate-the-efficiency-of-antigen-60-a60-protein-from-bcg-strain-of-mycobacterium-bovis-as-a-diagnostic-antigen
#17
Nafiseh Shakibamehr, Nader Mosavari, Mahdi Babaie, Samerand Reshady
OBJECTIVE/BACKGROUND: Tuberculosis (TB) is one of the most common infectious diseases in Iran and around the world. Diagnosis of this disease in many cases is difficult and often requires the use of paraclinical methods. Current diagnostic methods are either too slow or lack enough sensitivity or specificity. Several mycobacterial antigens are involved in the complex interaction with the immune system of the host. They can be helpful for mycobacteria diagnosis. Antigen 60 (A60) is a thermostable antigen found in the cytosol of Mycobacterium bovis and Mycobacterium tuberculosis...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043566/interferon-%C3%AE-assay-a-high-sensitivity-specific-and-appropriate-method-for-detection-of-bovine-tuberculosis-in-cattle
#18
R Keshavarz, N Mosavari, M M Geravand, Keyvan Tadayon, R A Pajoohi, K Solemani, M Ameri, G V Gilani
Bovine tuberculosis (TB) is an important zoonotic disease that is caused by Mycobacterium bovis. Eradication efforts in developed countries have reduced the prevalence of this disease significantly. TB can be difficult to diagnose based only on the clinical signs; therefore, it is usually diagnosed in the field with the tuberculin skin test and diagnostic blood tests, including the lymphocyte proliferation assay, the interferon (IFN)-γ assay, and enzyme-linked immunosorbent assay. The aim of this study was to compare the tuberculin and IFN-γ tests...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043531/evaluation-of-the-kudoh-method-for-mycobacterial-culture-gambia-experience
#19
Tijan Jobarteh, Jacob Otu, Ensa Gitteh, Francis Mendy, Tutty I Faal-Jawara, Boatema Ofori-Anyinam, Abigail Ayorinde, Ousman Secka, Martin Antonio, Florian Gehre
OBJECTIVE/BACKGROUND: To evaluate the Kudoh swab method for improving laboratory diagnosis of tuberculosis (TB) in Gambia. METHODS: A total of 75 sputa (50 smear positive and 25 smear negative) were examined. Sputum samples were collected from leftover routine samples from the Medical Research Council Unit, Gambia TB Diagnostic Laboratory. The samples were processed using the standard N-acetyl-l-cysteine-NaOH (NALC-NaOH) methods currently used and Kudoh swab method...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043530/rapid-detection-of-mycobacterium-tuberculosis-complex-in-sputum-samples-using-pure-tb-lamp-assay
#20
K N'guessan, K Horo, I Coulibaly, J Adegbele, N Kouame-Adjei, H Seck-Angu, A Guei, J Kouakou, M Dosso
OBJECTIVE/BACKGROUND: Lack of rapid and accurate diagnostic testing is a critical obstacle to global tuberculosis (TB) control. Sensitivity of sputum smear microscopy (SSM) is not optimal; however, it remains the most prevalent tool for TB confirmation in poor countries. As a part of passive case finding of TB detection, this study was conducted to determine the clinical performance of PURE TB-LAMP assay using liquid culture medium as the gold standard. METHODS: Centre Antituberculeux de Yopougon is one of the 17 intermediate Tuberculosis centers in Côte d'Ivoire...
December 2016: International Journal of Mycobacteriology
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