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https://www.readbyqxmd.com/read/29675788/recent-advances-in-tuberculosis-diagnostics-in-resource-limited-settings
#1
REVIEW
Mitsuko Seki, Chang-Ki Kim, Satoshi Hayakawa, Satoshi Mitarai
Smear-negative and drug-resistant cases of tuberculosis (TB) disease necessitate the development of new diagnostic methods, especially in resource-limited settings. To improve the current TB situations, sensitive and specific TB point-of-care tests (POCTs) should be developed. This review addresses the current status of TB, novel diagnostic methodologies for TB, and the impact of those new diagnostics on TB control in such situations. Moreover, the perspective of TB management based on laboratory examinations is described...
April 19, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29675262/genotyping-and-molecular-detection-of-multidrug-resistant-mycobacterium-tuberculosis-among-tuberculosis-lymphadenitis-cases-in-addis-ababa-ethiopia
#2
O Zewdie, A Mihret, T Abebe, A Kebede, K Desta, A Worku, G Ameni
Multidrug-resistant tuberculosis (MDR-TB) has emerged as a major public health problem. Drug-resistance surveillance data show that 3.9% of new and 21% of previously treated TB cases were estimated to have had rifampicin/ multidrug-resistant tuberculosis (MDR/RR-TB) in 2015. This implies that the MDR-TB is increasing alarmingly. Hence, a better understanding of drug resistance mechanisms and genotypes associated with multidrug resistance in M. tuberculosis is crucial for improving diagnostic and therapeutic methods to treat individuals with MDR-TB...
January 2018: New Microbes and New Infections
https://www.readbyqxmd.com/read/29664027/mycobacteria-derived-biomarkers-for-tuberculosis-diagnosis
#3
REVIEW
Magdalena Druszczynska, Sebastian Wawrocki, Rafal Szewczyk, Wieslawa Rudnicka
Tuberculosis (TB) remains an escalating problem worldwide. The current diagnostic methods do not always guarantee reliable diagnosis. TB treatment is a time-consuming process that requires the use of several chemotherapeutics, to which mycobacteria are becoming increasingly resistant. This article focuses on the potential utility of biomarkers of mycobacterial origin with potential implications for TB diagnosis. Properly standardized indicators could become new diagnostic tools, improving and streamlining the identification of Mycobacterium tuberculosis infection and the implementation of appropriate therapy...
December 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29662282/the-role-of-mgit-960-culture-medium-in-resolving-the-diagnostic-dilemma-for-genital-tuberculosis-patients-presenting-with-infertility
#4
Nidhi Jindal, Shalini Gainder, Lakhbir Kaur Dhaliwal, Sunil Sethi
Background: The purpose of this study was to assess the utility of Mycobacteria Growth Indicator Tube (MGIT) 960 culture medium for the diagnosis of genital tuberculosis (GTB) in women presenting with infertility. Methods: The premenstrual endometrial biopsy samples in 300 women presenting with primary and secondary infertility were subjected to AFB smear method, histopathological examination and culture on LJ and MGIT 960 media. Detection rates were compared for diagnostic modalities and their combinations...
April 2018: Journal of Obstetrics and Gynaecology of India
https://www.readbyqxmd.com/read/29653190/evaluation-of-whole-genome-sequencing-and-software-tools-for-drug-susceptibility-testing-of-mycobacterium-tuberculosis
#5
Janko van Beek, Marjo Haanperä, Pieter W Smit, Silja Mentula, Hanna Soini
OBJECTIVES: Culture-based assays are currently the gold standard for drug susceptibility testing (DST) for Mycobacterium tuberculosis. They provide good sensitivity and specificity, but are time-consuming. The objective of this study was to evaluate whether whole genome sequencing (WGS), combined with software tools for data analysis, can replace routine culture based assays for drug susceptibility testing of M. tuberculosis. METHODS: M. tuberculosis cultures sent to the Finnish mycobacterial reference laboratory in 2014 (n=211) were phenotypically tested by Mycobacteria Growth Indicator Tube (MGIT) for first line drug susceptibilities...
April 10, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29650047/care-cascade-for-targeted-tuberculosis-testing-and-linkage-to-care-in-homeless-populations-in-the-united-states-a-meta-analysis
#6
Andrea Parriott, Mohsen Malekinejad, Amanda P Miller, Suzanne M Marks, Hacsi Horvath, James G Kahn
BACKGROUND: Homelessness increases the risk of tuberculosis (TB) disease and latent TB infection (LTBI), but persons experiencing homelessness often lack access to testing and treatment. We assessed the yield of TB testing and linkage to care for programs targeting homeless populations in the United States. METHODS: We conducted a comprehensive search of peer-reviewed and grey literature, adapting Cochrane systematic review methods. Two reviewers independently assessed study eligibility and abstracted key data on the testing to care cascade: number of persons reached, recruited for testing, tested for LTBI, with valid test results, referred to follow-up care, and initiating care...
April 12, 2018: BMC Public Health
https://www.readbyqxmd.com/read/29599184/rapid-diagnosis-of-pulmonary-tuberculosis-by-combined-molecular-and-immunological-methods
#7
Claudia Jafari, Ioana D Olaru, Franziska Daduna, Martin Ernst, Jan Heyckendorf, Christoph Lange, Barbara Kalsdorf
Diagnosing pulmonary tuberculosis (TB) may be delayed until culture results become available.We ascertained the accuracy of a stepwise diagnostic algorithm for the rapid diagnosis of pulmonary TB by GeneXpert from sputum and/or bronchoalveolar-lavage (BAL) followed by a M. tuberculosis- specific BAL-enzyme-linked immunospot (ELISpot) assay in patients with a suspected diagnosis of pulmonary TB at a clinical referral center in Germany.Among 166 patients with a presumptive diagnosis of pulmonary TB, 81 cases were confirmed by M...
March 29, 2018: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29594708/endoscopic-ultrasound-guided-fine-needle-aspiration-of-enlarged-adrenals-in-patients-with-pyrexia-of-unknown-origin-a-single-center-experience-of-52-cases
#8
Rinkesh K Bansal, Narendra S Choudhary, Saurabh K Patle, Amit Agarwal, Gagandeep Kaur, Haimanti Sarin, Rajesh Puri
BACKGROUND: Fine-needle aspiration (FNA) of adrenals is needed in patients with pyrexia of unknown origin (PUO) and adrenal enlargement in absence of other diagnostic clues. Adrenals are easily accessible by endoscopic ultrasound (EUS) due to proximity; however, there is no systemic study available on FNA of adrenals in patients with PUO. The aim of this study was to evaluate the diagnostic yield and safety of EUS-FNA of enlarged adrenal in patients with PUO. METHODS: Data was analyzed from October 2010 to September 2016 at a single tertiary care center in northern India...
March 28, 2018: Indian Journal of Gastroenterology: Official Journal of the Indian Society of Gastroenterology
https://www.readbyqxmd.com/read/29593688/a-comprehensive-evaluation-of-xpert-mtb-rif-assay-with-bronchoalveolar-lavage-fluid-as-a-single-test-or-combined-with-conventional-assays-for-diagnosis-of-pulmonary-tuberculosis-in-china-a-two-center-prospective-study
#9
Xiaofu Pan, Shoufeng Yang, Margaret A Deighton, Yue Qu, Liang Hong, Feifei Su
Introduction: The Xpert MTB/RIF is recommended by the World Health Organization as a first line rapid test for the diagnosis of pulmonary tuberculosis (TB); however, China does not routinely use this test, partially due to the lack of a sufficient number of systematic evaluations of this assay in local patients. The aims of this study were to comprehensively assess the diagnostic performance of Xpert MTB/RIF, either alone or in combination with conventional assays for the diagnosis of pulmonary TB in adult Chinese patients...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29584796/should-sputum-negative-presumptive-tb-patients-be-actively-followed-to-identify-missing-cases-in-india
#10
S Waikar, A Pathak, V Ghule, A Kapoor, K Sagili, E R Babu, S Chadha
Setting: Sputum smear microscopy, the primary diagnostic tool used for diagnosis of tuberculosis (TB) in India's Revised National TB Control Programme (RNTCP), has low sensitivity, resulting in a significant number of TB cases reported as sputum-negative. As the revised guidelines pose challenges in implementation, sputum-negative presumptive TB (SNPT) patients are subjected to 2 weeks of antibiotics, followed by chest X-ray (CXR), resulting in significant loss to care among these cases. Objective: To determine whether reducing delays in CXR would yield additional TB cases and reduce initial loss to follow-up for diagnosis among SNPT cases...
December 21, 2017: Public Health Action
https://www.readbyqxmd.com/read/29570492/when-do-coinfections-matter
#11
Andrew J McArdle, Anna Turkova, Aubrey J Cunnington
PURPOSE OF REVIEW: Advances in diagnostic methods mean that coinfections are increasingly being detected in clinical practice, yet their significance is not always obvious. In parallel, basic science studies are increasingly investigating interactions between pathogens to try to explain real-life observations and elucidate biological mechanisms. RECENT FINDINGS: Coinfections may be insignificant, detrimental, or even beneficial, and these outcomes can occur through multiple levels of interactions which include modulation of the host response, altering the performance of diagnostic tests, and drug-drug interactions during treatment...
March 22, 2018: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/29562991/which-attributes-within-target-product-profiles-for-tuberculosis-diagnostics-are-the-most-important-to-focus-on
#12
T Adepoyibi, L Lilis, H Greb, D Boyle
SETTING: New diagnostic tools for tuberculosis (TB) are urgently needed. To guide investment decisions, sufficient information regarding which test attributes are the most important for users and decision makers interested in introducing TB diagnostics should be made available. OBJECTIVE: To rank test attributes in a target product profile (TPP) in order of importance for a sputum smear replacement test for TB. DESIGN: An online survey was administered among 33 participants representing 14 of the 22 TB high-burden countries...
April 1, 2018: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/29562934/diagnostic-performance-and-problem-analysis-of-commercial-tuberculosis-antibody-detection-kits-in-china
#13
Xue-Juan Bai, You-Rong Yang, Jian-Qin Liang, Hui-Ru An, Jie Wang, Yan-Bo Ling, Zhong-Yuan Wang, Xue-Qiong Wu
BACKGROUND: The diagnosis of bacterium-negative pulmonary tuberculosis (TB) and extra-pulmonary TB is challenging clinically. The detection of the anti-TB antibody has an important, auxiliary, clinical diagnostic value. Therefore, TB antibody detection kits should be screened and evaluated, and the reagents with the highest sensitivity and specificity should be chosen and used clinically. METHODS: The diagnostic performance of 7 commercially available TB antibody detection kits (kits A, B, C, D, E, F and G) based on the gold immunoassay detection of immunoglobulin (Ig) G or IgM antibodies were simultaneously evaluated and compared in 62 TB cases and 56 non-TB cases in a laboratory...
March 22, 2018: Military Medical Research
https://www.readbyqxmd.com/read/29535695/identification-of-a-novel-serum-biomarker-for-tuberculosis-infection-in-chinese-hiv-patients-by-itraq-based-quantitative-proteomics
#14
Cong Chen, Tao Yan, Liguo Liu, Jianmin Wang, Qi Jin
Tuberculosis (TB) is a major comorbidity in HIV patients as well as a serious co-epidemic. Traditional detection methods are not effective or sensitive for the detection of Mycobacterium tuberculosis at the early stage. TB has become a major cause of lethal on HIV patients. We employed isobaric tags for relative and absolute quantitation (iTRAQ) technology to identify the different host responses in HIV-noTB and HIV-TB patients' sera. Given the diversity of HIV subtypes, which results in a variety of host responses in different human populations, we focused on the Chinese patients...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29529901/evaluation-of-risk-factors-for-false-negative-results-with-an-antigen-specific-peripheral-blood-based-quantitative-t-cell-assay-t-spot-%C3%A2-tb-in-the-diagnosis-of-active-tuberculosis-a-large-scale-retrospective-study-in-china
#15
Chi Yang, Shaojun Zhang, Lan Yao, Lin Fan
Objective To investigate the diagnostic efficacy of an interferon-γ release assay, T-SPOT® . TB, for diagnosing active tuberculosis (TB) and to identify risk factors for false-negative results. Methods This retrospective study enrolled consecutive patients with active TB and with non-TB respiratory diseases to evaluate the risk factors for false-negative results when using the T-SPOT® . TB assay for the diagnosis of active TB. Patients with active TB were categorized as having confirmed pulmonary TB, clinically diagnosed pulmonary TB or extrapulmonary TB (EPTB)...
January 1, 2018: Journal of International Medical Research
https://www.readbyqxmd.com/read/29526443/diagnostic-performance-of-the-rt-qpcr-method-targeting-85b-mrna-in-the-diagnosis-of-pulmonary-mycobacterium-tuberculosis-infection
#16
Mehmet Demirci, Suat Saribas, Nigar Ozer, Sezer Toprak, Emel Caglar, Gonenc Ortakoylu, Pelin Yuksel, Gulsel Ayaz, Hrisi Bahar Tokman, Omer Uysal, Harika Oyku Dinc, Tevhide Ziver, Bekir Kocazeybek
BACKGROUND: Several nucleic acid amplification techniques (IS6110, 16S rRNA, and 85B mRNA) were developed for the rapid, direct detection of Mycobacterium tuberculosis. We aimed to assess the diagnostic performance of 85B mRNA-based RT-qPCR by comparing with the real-time PCR COBAS TaqMan MTB Kit while using the BACTEC MGIT 960 method as the gold standard. METHODS: 60 patients with confirmed pulmonary TB and 60 individuals without TB were included as the study and control groups, respectively...
March 8, 2018: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/29525564/selective-detection-of-co-2-by-fluorescent-nano-probe-diagnostic-approach-for-analysis-of-environmental-samples-and-biological-activities
#17
Prasad G Mahajan, Nilam C Dige, Netaji K Desai, Shivajirao R Patil, Vijay V Kondalkar, Seong-Karp Hong, Ki Hwan Lee
Nowadays scientist over the world are engaging to put forth improved methods to detect metal ion in an aqueous medium based on fluorescence studies. A simple, selective and sensitive method was proposed for detection of Co2+ ion using fluorescent organic nanoparticles. We synthesized a fluorescent small molecule viz. 4,4'-{benzene-1,4-diylbis-[(Z)methylylidenenitrilo]}dibenzoic acid (BMBA) to explore its suitability as sensor for Co2+ ion and biocompatibility in form of nanoparticles. Fluorescence nanoparticles (BMBANPs) prepared by simple reprecipitation method...
March 3, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29523324/screening-and-identification-of-four-serum-mirnas-as-novel-potential-biomarkers-for-cured-pulmonary-tuberculosis
#18
Chong Wang, Su Yang, Chang-Ming Liu, Ting-Ting Jiang, Zhong-Liang Chen, Hui-Hui Tu, Lian-Gen Mao, Zhong-Jie Li, Ji-Cheng Li
Rapid and efficient methods for the determination of cured pulmonary tuberculosis (TB) are lacking. We screened serum miRNAs using the Solexa sequencing method among untreated TB patients, two-month treated TB patients, cured TB patients, and healthy controls. A total of 100 differentially expressed miRNAs were identified in cured TB patients, including 37 up-regulated (fold change >1.50, P < 0.05) and 63 down-regulated (fold change <0.60, P < 0.05) miRNAs. Gene ontology (GO) enrichment analysis revealed that most of the predicted genes were present in the nucleus with a strong protein binding function...
January 2018: Tuberculosis
https://www.readbyqxmd.com/read/29523307/acquaintance-to-artificial-neural-networks-and-use-of-artificial-intelligence-as-a-diagnostic-tool-for-tuberculosis-a-review
#19
REVIEW
Payal Dande, Purva Samant
Tuberculosis [TB] has afflicted numerous nations in the world. As per a report by the World Health Organization [WHO], an estimated 1.4 million TB deaths in 2015 and an additional 0.4 million deaths resulting from TB disease among people living with HIV, were observed. Most of the TB deaths can be prevented if it is detected at an early stage. The existing processes of diagnosis like blood tests or sputum tests are not only tedious but also take a long time for analysis and cannot differentiate between different drug resistant stages of TB...
January 2018: Tuberculosis
https://www.readbyqxmd.com/read/29523100/loss-to-follow-up-and-delay-to-treatment-initiation-in-pakistan-s-national-tuberculosis-control-programme
#20
Syed Mustafa Ali, Farah Naureen, Arif Noor, Irum Fatima, Kerri Viney, Muhammad Ishaq, Naveed Anjum, Aamna Rashid, Ghulam Rasool Haider, Muhammad Aamir Khan, Javariya Aamir
BACKGROUND: Researchers and policy-makers have identified loss to follow-up as a major programmatic problem. Therefore, the objective of this study is to quantify TB related pre-treatment loss to follow up and treatment delay in private sector health care facilities in Pakistan. METHODS: This was a retrospective, descriptive cohort study using routinely collected programmatic data from TB referral, diagnosis and treatment registers. Data from 48 private healthcare facilities were collected using an online questionnaire prepared in ODK Collect, for the period October 2015 to March 2016...
March 9, 2018: BMC Public Health
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