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https://www.readbyqxmd.com/read/27887611/the-performance-of-interferon-gamma-release-assay-in-nontuberculous-mycobacterial-diseases-a-retrospective-study-in-china
#1
Mao-Shui Wang, Jun-Li Wang, Xin-Feng Wang
BACKGROUND: The interferon-gamma release assay (IGRA) is more specific than the tuberculin skin test to discriminate between tuberculosis (TB) and nontuberculous mycobacterial (NTM) diseases. Here we performed a retrospective study to evaluate the performance of the T-SPOT.TB in patients with NTM diseases. METHODS: Between March, 2013 and Nov, 2015, a total of 58 patients with NTM diseases had a T-SPOT.TB performed were enrolled, 30 patients had definite NTM diseases, 28 had probable diseases...
November 25, 2016: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/27882115/retrospective-analysis-of-seven-breast-tuberculosis-cases
#2
Beyhan Çakar, Aydin Çiledağ
The present study aimed to evaluate the demographic data, diagnostic methods, therapeutic regimens and duration of therapy in 7 breast tuberculosis (BTB) cases. The data of BTB cases treated between January 2006 and December 2013 were retrospectively evaluated, with a total of 648 tuberculosis (TB) cases recorded during the 8-year period. Among these cases, 296 patients (50%) suffered from pulmonary TB, 278 (43%) from non-pulmonary TB and 45 (7%) from PTB plus NPTB. In total, 7 BTB were diagnosed, which constituted 1...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27879161/neopterin-and-oxidative-stress-markers-in-the-diagnosis-of-extrapulmonary-tuberculosis
#3
Nisha Goyal, Bineeta Kashyap, N P Singh, Iqbal R Kaur
BACKGROUND: Extrapulmonary tuberculosis (EPTB) often presents with non-specific signs and symptoms. Further the paucibacillary nature of extrapulmonary specimens and irregular distribution of bacilli lower the sensitivity of conventional diagnostic methods making EPTB, a diagnostic dilemma. Objective To study neopterin, protein carbonyl and malondialdehyde in extrapulmonary tuberculosis. METHODS: 69 clinically confirmed cases with an equal number of age and sex matched healthy controls were enrolled...
November 23, 2016: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/27872016/tuberculosis-eradication-versus-control
#4
Marco Schito, Debra Hanna, Alimuddin Zumla
According to the World Health Organization (WHO), 10.4 million people succumbed to tuberculosis (TB) in 2015 and is now the number one cause of death world-wide from a preventable infectious disease. Bold vision is needed from global leaders and plans have been proposed to end the TB epidemic. However enthusiasm must be matched by tangible and achievable goals based on the science and available funding. In order to reach the target and goals set by the WHO's End-TB Strategy, the challenges for TB eradication need to be addressed...
November 18, 2016: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27865380/highly-sensitive-sequence-specific-qpcr-detection-of-mycobacterium-tuberculosis-complex-in-respiratory-specimens
#5
Jennifer L Reed, Zachary J Walker, Debby Basu, Veronica Allen, Mark P Nicol, David M Kelso, Sally M McFall
Nucleic acid amplification tests for Mycobacterium tuberculosis (MTB) detection from sputum are highly sensitive and specific with smear microscopy positive specimens, but their sensitivity with smear-negative/culture-positive specimens is much lower; therefore, these tests cannot rule out a tuberculosis diagnosis. Co-extraction of PCR inhibitors may be a cause of decreased test sensitivity. Here the design and early validation of a MTB screening assay with sample preparation and qPCR methods designed to specifically address this diagnostic gap is reported...
December 2016: Tuberculosis
https://www.readbyqxmd.com/read/27858862/utility-of-t-cell-interferon-%C3%AE-release-assays-for-the-diagnosis-of-female-genital-tuberculosis-in-a-tertiary-referral-hospital-in-beijing-china
#6
Xiaoqing Liu, Sainan Bian, Xinhe Cheng, Wenze Wang, Qinjie Tian, Lifan Zhang, Yueqiu Zhang, Xiaochun Shi, Yao Zhang, Zhiyong Liang
Diagnosis of female genital tuberculosis (FGTB) remains a challenge. The aim of this study was to evaluate the diagnostic value of T-SPOT.TB on peripheral blood mononuclear cells (PBMCs) for diagnosing FGTB in an area with high TB burden.Patients with suspected FGTB were enrolled consecutively between August 2010 and August 2015. T-SPOT.TB on PBMCs and histopathology were performed in all patients. T-SPOT.TB results were evaluated against patients' final diagnosis of FGTB which was made based on clinical manifestations, radiology, microbiological and histopathological evaluation, and response to anti-TB treatment...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27825314/diagnostic-performance-of-smear-microscopy-and-incremental-yield-of-xpert-in-detection-of-pulmonary-tuberculosis-in-rwanda
#7
Jean Claude Semuto Ngabonziza, Willy Ssengooba, Florence Mutua, Gabriela Torrea, Augustin Dushime, Michel Gasana, Emmanuel Andre, Schifra Uwamungu, Alaine Umubyeyi Nyaruhirira, Dufton Mwaengo, Claude Mambo Muvunyi
BACKGROUND: Tuberculosis control program of Rwanda is currently phasing in light emitting diode-fluorescent microscopy (LED-FM) as an alternative to Ziehl-Neelsen (ZN) smear microscopy. This, alongside the newly introduced Xpert (Cepheid, Sunnyvale, CA, USA) is expected to improve diagnosis of tuberculosis and detection of rifampicin resistance in patients at health facilities. We assessed the accuracy of smear microscopy and the incremental sensitivity of Xpert at tuberculosis laboratories in Rwanda...
November 8, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27798774/detection-of-transrenal-dna-for-the-diagnosis-of-pulmonary-tuberculosis-and-treatment-monitoring
#8
Ines Labugger, Jan Heyckendorf, Stefan Dees, Emilia Häussinger, Christian Herzmann, Thomas A Kohl, Elvira Richter, Eric Rivera-Milla, Christoph Lange
PURPOSE: Molecular diagnostics of patients with MTB tuberculosis from urine samples. METHODS: We developed a new molecular assay based on the detection of M. tuberculosis-specific transrenal DNA (trDNA) and tested it for the diagnosis of active tuberculosis at the initiation of anti-tuberculosis therapy and during treatment follow-up. RESULTS: The overall sensitivity of trDNA was 96 and 100% when smear-microscopy and trDNA was combined. In a subset of TB treatment naïve patients (n = 11) sensitivity and specificity of trDNA was 64 and 100%, respectively...
October 31, 2016: Infection
https://www.readbyqxmd.com/read/27798613/genomic-diversity-in-autopsy-samples-reveals-within-host-dissemination-of-hiv-associated-mycobacterium-tuberculosis
#9
Tami D Lieberman, Douglas Wilson, Reshma Misra, Lealia L Xiong, Prashini Moodley, Ted Cohen, Roy Kishony
Mycobacterium tuberculosis remains a leading cause of death worldwide, especially among individuals infected with HIV. Whereas phylogenetic analysis has revealed M. tuberculosis spread throughout history and in local outbreaks, much less is understood about its dissemination within the body. Here we report genomic analysis of 2,693 samples collected post mortem from lung and extrapulmonary biopsies of 44 subjects in KwaZulu-Natal, South Africa, who received minimal antitubercular treatment and most of whom were HIV seropositive...
December 2016: Nature Medicine
https://www.readbyqxmd.com/read/27798497/recent-advances-in-the-diagnosis-and-management-of-tuberculous-meningitis
#10
Nguyen T H Mai, Guy E Thwaites
PURPOSE OF REVIEW: Tuberculous meningitis is a devastating infection that is hard to diagnose and treat. We have reviewed tuberculous meningitis original research published within the past 18 months, selecting studies which we consider have most advanced knowledge. RECENT FINDINGS: We review advances in diagnostic methods, anti-tuberculosis chemotherapy, and the common complications of tuberculous meningitis. New commercial molecular diagnostic tests, such as GeneXpert MTB/RIF, have an important role in tuberculous meningitis diagnosis, but as with all other available tests, they lack sensitivity and cannot rule out the disease...
October 27, 2016: Current Opinion in Infectious Diseases
https://www.readbyqxmd.com/read/27796109/animal-adapted-members-of-the-i-mycobacterium-tuberculosis-i-complex-endemic-to-the-southern-african-subregion
#11
Charlene Clarke, Paul Van Helden, Michele Miller, Sven Parsons
Members of the Mycobacterium tuberculosis complex (MTC) cause tuberculosis (TB) in both animals and humans. In this article, three animal-adapted MTC strains that are endemic to the southern African subregion - that is, Mycobacterium suricattae, Mycobacterium mungi, and the dassie bacillus - are reviewed with a focus on clinical and pathological presentations, geographic distribution, genotyping methods, diagnostic tools and evolution. Moreover, factors influencing the transmission and establishment of TB pathogens in novel host populations, including ecological, immunological and genetic factors of both the host and pathogen, are discussed...
April 26, 2016: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/27790312/magnetic-resonance-imaging-and-genexpert-a-rapid-and-accurate-diagnostic-tool-for-the-management-of-tuberculosis-of-the-spine
#12
Ayush Sharma, Harvinder Singh Chhabra, Rajat Mahajan, Tarun Chabra, Sahil Batra
STUDY DESIGN: Retrospective study. PURPOSE: The aim of this study was to analyze various diagnostic tools, including GeneXpert, for the management of tuberculosis of the spine. OVERVIEW OF LITERATURE: Traditional diagnostic methods of microscopy, histology, and culture have low sensitivity and specificity for the management of tuberculosis of the spine. METHODS: Of the 262 treated cases of spinal tuberculosis, data on 1 year follow-up was available for 217 cases...
October 2016: Asian Spine Journal
https://www.readbyqxmd.com/read/27788025/a-laboratory-based-analysis-of-nontuberculous-mycobacterial-lung-disease-in-japan-from-2012-to-2013
#13
Kozo Morimoto, Naoki Hasegawa, Kiyohiko Izumi, Ho Namkoong, Kazuhiro Uchimura, Takashi Yoshiyama, Yoshihiko Hoshino, Atsuyuki Kurashima, Jun Sokunaga, Shunsuke Shibuya, Masahiro Shimojima, Manabu Ato, Satoshi Mitarai
RATIONALE: Since 2010, studies that utilize mycobacterial examination results have been widely used to survey nontuberculous mycobacterial (NTM) lung disease. OBJECTIVES: Our study aims to reveal the clinical and epidemiological status of NTM lung disease in Japan. METHODS: All data on the isolation and identification of mycobacteria in 2012 and 2013 were obtained from three dominant commercial laboratories in Japan. Pulmonary disease were defined based on the bacteriological diagnostic criteria issued by the American Thoracic Society and the Infectious Diseases Society of America (ATS/IDSA)...
October 27, 2016: Annals of the American Thoracic Society
https://www.readbyqxmd.com/read/27766798/to-study-efficacy-of-medical-thoracoscopy-in-undiagnosed-pleural-effusions
#14
Sachin S Dole, Gauri P Godbole, Himanshu S Pophale
BACKGROUND: Medical thoracoscopy has received interest in recent past for diagnostic as well as therapeutic uses. In this study we describe our experience with diagnostic medical thoracoscopy in pleural effusions of undiagnosed etiology. OBJECTIVE: 1.To detect diagnostic yield of medical thoracoscopy in pleural effusions of unknown etiology. 2.To find complication rate in these patients. METHODOLOGY: In a retrospective analysis of thoracoscopic procedures performed between March 2011 and December 2014, diagnostic yield and complications of thoracoscopic pleural biopsy for achieving a diagnosis in undiagnosed pleural effusions were evaluated...
October 2016: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/27760181/discordance-between-mtb-rif-and-real-time-tuberculosis-specific-polymerase-chain-reaction-assay-in-bronchial-washing-specimen-and-its-clinical-implications
#15
Yong Suk Jo, Ju-Hee Park, Jung Kyu Lee, Eun Young Heo, Hee Soon Chung, Deog Kyeom Kim
The prevalence and clinical implications of discordance between Xpert MTB/RIF assays and the AdvanSure TB/NTM real-time polymerase chain reaction (PCR) for bronchial washing specimens have not been studied in pulmonary TB (PTB) patients. The discordant proportion and its clinical impact were evaluated in 320 patients from the bronchoscopy registry whose bronchial washing specimens were tested simultaneously with Xpert MTB/RIF and the TB/NTM PCR assay for three years, and the accuracy of the assays, including the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV), were studied...
2016: PloS One
https://www.readbyqxmd.com/read/27739665/design-of-selective-substrates-and-activity-based-probes-for-hydrolase-important-for-pathogenesis-1-hip1-from-mycobacterium-tuberculosis
#16
Christian S Lentz, Alvaro A Ordonez, Paulina Kasperkiewicz, Florencia La Greca, Anthony J O'Donoghue, Christopher J Schulze, James C Powers, Charles S Craik, Marcin Drag, Sanjay K Jain, Matthew Bogyo
Although serine proteases are important mediators of Mycobacterium tuberculosis (Mtb) virulence, there are currently no tools to selectively block or visualize members of this family of enzymes. Selective reporter substrates or activity-based probes (ABPs) could provide a means to monitor infection and response to therapy using imaging methods. Here, we use a combination of substrate selectivity profiling and focused screening to identify optimized reporter substrates and ABPs for the Mtb "Hydrolase important for pathogenesis 1" (Hip1) serine protease...
July 15, 2016: ACS Infectious Diseases
https://www.readbyqxmd.com/read/27734027/blood-transcriptomic-diagnosis-of-pulmonary-and-extrapulmonary-tuberculosis
#17
Jennifer K Roe, Niclas Thomas, Eliza Gil, Katharine Best, Evdokia Tsaliki, Stephen Morris-Jones, Sian Stafford, Nandi Simpson, Karolina D Witt, Benjamin Chain, Robert F Miller, Adrian Martineau, Mahdad Noursadeghi
BACKGROUND. Novel rapid diagnostics for active tuberculosis (TB) are required to overcome the time delays and inadequate sensitivity of current microbiological tests that are critically dependent on sampling the site of disease. Multiparametric blood transcriptomic signatures of TB have been described as potential diagnostic tests. We sought to identify the best transcript candidates as host biomarkers for active TB, extend the evaluation of their specificity by comparison with other infectious diseases, and to test their performance in both pulmonary and extrapulmonary TB...
October 6, 2016: JCI Insight
https://www.readbyqxmd.com/read/27730659/understanding-the-effects-of-conditional-dependence-in-research-studies-involving-imperfect-diagnostic-tests
#18
Zhuoyu Wang, Nandini Dendukuri, Lawrence Joseph
When two imperfect diagnostic tests are carried out on the same subject, their results may be correlated even after conditioning on the true disease status. While past work has focused on the consequences of ignoring conditional dependence, the degree to which conditional dependence can be induced has not been systematically studied. We examine this issue in detail by introducing a hypothetical missing covariate that affects the sensitivities of two imperfect dichotomous tests. We consider four forms for this covariate, normal, uniform, dichotomous and trichotomous...
October 11, 2016: Statistics in Medicine
https://www.readbyqxmd.com/read/27729258/new-directions-using-reporter-enzyme-fluorescence-ref-as-a-tuberculosis-diagnostic-platform
#19
Preeti Sule, Ronak Tilvawala, Parnia Behinaein, Grant K Walkup, Jeffrey D Cirillo
Although tuberculosis (TB) is one of the most common causes of morbidity and mortality in humans worldwide and diagnostic methods have been in place for more than 100 years, diagnosis remains a challenge. The main problems with diagnosis relate to the time needed to obtain a definitive result, difficulty in obtaining sputum, the primary clinical material used, and the ability of the causative agent, Mycobacterium tuberculosis, to cause disease in nearly any tissue within the body. In order to decrease incidence of TB, discovery of a novel interventions will be required, since current technologies have only been able to control numbers of infections, not reduce them...
September 28, 2016: Tuberculosis
https://www.readbyqxmd.com/read/27726808/mycobacteria
#20
Patricia J Simner, Gail L Woods, Nancy L Wengenack
The immunocompromised host is at increased risk of Mycobacterium tuberculosis complex and nontuberculous mycobacteria infection. Although Mycobacterium tuberculosis complex is a significant mycobacterial pathogen, nontuberculous mycobacteria causes substantial disease in those with suppressed immune responses. Mycobacterial infections can cause significant morbidity and mortality in this patient population, and rapid identification and susceptibility testing of the mycobacterial species is paramount to patient management and outcomes...
August 2016: Microbiology Spectrum
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