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diagnostic methods tuberculosis

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https://www.readbyqxmd.com/read/29451594/nontuberculous-mycobacteria-in-milk-from-positive-cows-in-the-intradermal-comparative-cervical-tuberculin-test-implications-for-human-tuberculosis-infections
#1
Carmen Alicia Daza Bolaños, Marília Masello Junqueira Franco, Antonio Francisco Souza Filho, Cássia Yumi Ikuta, Edith Mariela Burbano-Rosero, José Soares Ferreira Neto, Marcos Bryan Heinemann, Rodrigo Garcia Motta, Carolina Lechinski de Paula, Amanda Bonalume Cordeiro de Morais, Simony Trevizan Guerra, Ana Carolina Alves, Fernando José Paganini Listoni, Márcio Garcia Ribeiro
Although the tuberculin test represents the main in vivo diagnostic method used in the control and eradication of bovine tuberculosis, few studies have focused on the identification of mycobacteria in the milk from cows positive to the tuberculin test. The aim of this study was to identify Mycobacterium species in milk samples from cows positive to the comparative intradermal test. Milk samples from 142 cows positive to the comparative intradermal test carried out in 4,766 animals were aseptically collected, cultivated on Lowenstein-Jensen and Stonebrink media and incubated for up to 90 days...
2018: Revista do Instituto de Medicina Tropical de São Paulo
https://www.readbyqxmd.com/read/29445235/development-and-application-of-a-rapid-mycobacterium-tuberculosis-detection-technique-using-polymerase-spiral-reaction
#2
Wei Liu, Dayang Zou, Xiaoming He, Da Ao, Yuxin Su, Zhan Yang, Simo Huang, Qinghe Zhao, Yue Tang, Wen Ma, Yongfeng Lu, Jing Wang, Xinjing Wang, Liuyu Huang
Mycobacterium tuberculosis is an age-old bacterium that is difficult to eliminate. A simple and rapid diagnostic method is of great importance to prevent the spread of M. tuberculosis. Here, we developed a low-cost rapid M. tuberculosis nucleic acid detection technique, named GenePop, which enabled the storage and transport of M. tuberculosis diagnostic reagent at ambient temperatures, without the need for professional operations or expensive instrumentation. Using a vitrification method, we vitrified heat-unstable components onto the cap of a reaction tube, and placed heat-stable components at the bottom of the reaction tube by sealing them with paraffin wax...
February 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29437632/time-to-multidrug-resistant-tuberculosis-treatment-initiation-in-association-with-treatment-outcomes-in-shanghai-china
#3
Yong Chen, Zhengan Yuan, Xin Shen, Jie Wu, Zheyuan Wu, Biao Xu
In high TB-burden countries like China, the diagnosis of multidrug-resistant tuberculosis (MDR-TB) using conventional drug susceptibility testing (DST) takes months, making treatment delay inevitable. Poor outcomes of MDR-TB might be associated with delayed, even inappropriate treatment. The purpose of this study was to investigate the time to MDR-TB treatment initiation, and to assess the association between early treatment and treatment outcomes. Between April 2011 and December 2014, this population-based, retrospective cohort study collected the demographic and clinical characteristics, and the drug susceptibility profiles of all registered MDR-TB patients in Shanghai, China...
February 5, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29429785/spinal-primary-central-nervous-system-lymphoma-case-report-and-literature-review
#4
REVIEW
Li Feng, Dingbang Chen, Hongyan Zhou, Cunzhou Shen, Haiyan Wang, Xunsha Sun, Xiulin Liang, Ling Chen
Primary central nervous system lymphoma (PCNSL) is a very rare tumor of increasing incidence. It is often misdiagnosed due to the unspecific presentation or unavailable biopsy, and results in poor prognosis. PCNSL involved the spinal cord is extremely sparse. Here we report a gentleman presented with one-year history of progressive tremor in the left limbs and slight dysarthria as well as three-month history of paraparesis, tinnitus and insomnia. MR images disclosed the swollen cerebellum and cauda equine, with contrast enhancement in both meninges and nerve roots...
February 8, 2018: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/29428408/evaluating-the-diagnostic-accuracy-of-the-xpert%C3%A2-mtb-rif-assay-on-bronchoalveolar-lavage-fluid-a-retrospective-study
#5
Yanjun Lu, Yaowu Zhu, Na Shen, Lei Tian, Ziyong Sun
OBJECTIVE: Limited data on the diagnostic accuracy of the Xpert® MTB/RIF assay using bronchoalveolar lavage fluid in patients with suspected pulmonary tuberculosis (PTB) have been reported in China. Therefore, we designed a retrospective study to evaluate the diagnostic accuracy of this assay. METHODS: Clinical, radiological, and microbiological characteristics of 238 PTB-suspected patients were retrospectively reviewed. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) for the diagnosis of active PTB were calculated for the Xpert® MTB/RIF assay using TB culture or final diagnosis based on clinical and radiological evaluation as a reference standard...
February 8, 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29427966/do-higher-cut-off-values-for-tuberculin-skin-test-increase-the-specificity-and-diagnostic-agreement-with-interferon-gamma-release-assays-in-immunocompromised-bacillus-calmette-gu%C3%A3-rin-vaccinated-patients
#6
Serhat Erol, Fatma Arslan Ciftci, Aydin Ciledag, Akin Kaya, Ozlem Ozdemir Kumbasar
PURPOSE: Immunocompromised patients with latent tuberculosis infection (LTBI) are at high risk of progression to active tuberculosis. Detection and treatment of LTBI in this group of patients are very important to control active tuberculosis. Tuberculin skin test (TST) and interferon gamma release assays (IGRAs) are two methods for detection of LTBI. Diagnostic agreement between two tests are poor especially in Bacillus Calmette-Guérin (BCG) vaccinated immunocompromised patients. In this study, we tried to figure out if the use of a higher cut-off for TST increases diagnostic agreement with IGRAs and TST specificity and or not...
February 7, 2018: Advances in Medical Sciences
https://www.readbyqxmd.com/read/29410366/performance-of-the-tb-lamp-diagnostic-assay-in-reference-laboratories-results-from-a-multicentre-study
#7
Thu Hang Pham, Jonathan Peter, Fernanda C Q Mello, Tommy Parraga, Nguyen Thi Ngoc Lan, Pamela Nabeta, Eloise Valli, Tatiana Caceres, Keertan Dheda, Susan E Dorman, Doris Hillemann, Christen M Gray, Mark D Perkins
OBJECTIVE: To evaluate the diagnostic performance of TB-LAMP, a manual molecular tuberculosis (TB) detection method, and provide comparison to the Xpert MTB/RIF assay. METHODS: In a large multicentre study, two sputum samples were collected from participants with TB symptoms in reference laboratories in Peru, South Africa, Brazil, and Vietnam. Each sample was tested with TB-LAMP. The reference standard consisted of four direct smears, four cultures, and clinical and radiological findings...
February 2, 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29406800/rapid-molecular-diagnostics-for-multi-drug-resistant-tuberculosis-in-india
#8
Rajeswari Ramachandran, M Muniyandi
Rapid molecular diagnostic methods help in the detection of TB and Rifampicin resistance. These methods detect TB early, are accurate and play a crucial role in reducing the burden of drug resistant tuberculosis. Areas covered: This review analyses rapid molecular diagnostic tools used in the diagnosis of MDR-TB in India, such as the Line Probe Assay and GeneXpert. We have discussed the burden of MDR-TB and the impact of recent diagnostic tools on case detection and treatment outcomes. This review also discusses the costs involved in establishing these new techniques in India...
February 6, 2018: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/29405982/simultaneous-detections-of-genetic-fragment-and-single-nucleotide-mutation-with-a-three-tiered-output-for-tuberculosis-diagnosis
#9
Shih-Ying Huang, Jia-Ru Chang, Yu-Chieh Liao, Horng-Yunn Dou, Min-Chieh Chuang
Tuberculosis (TB) remains one of the major infectious diseases worldwide. The pathogenic bacterium, Mycobacterium tuberculosis (M.tb), continuously evolves strains carrying drug-resistance genes, thus posing a growing challenge to TB prevention and treatment. We report a diagnostic system that uses a molecular beacon probe and an assistant strand as the core to simultaneously interact with an M.tb-specific fragment (in IS6110) and a single nucleotide substitution (SNS)-encoded segment (in rpoB) associated with drug resistance...
May 12, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29399559/molecular-diagnosis-of-tuberculosis
#10
REVIEW
Fariz Nurwidya, Diah Handayani, Erlina Burhan, Faisal Yunus
Tuberculosis (TB) is one of the leading causes of adult death in the Asia-Pacific Region, including Indonesia. As an infectious disease caused by Mycobacterium tuberculosis (MTB), TB remains a major public health issue especially in developing nations due to the lack of adequate diagnostic testing facilities. Diagnosis of TB has entered an era of molecular detection that provides faster and more cost-effective methods to diagnose and confirm drug resistance in TB cases, meanwhile, diagnosis by conventional culture systems requires several weeks...
January 2018: Chonnam Medical Journal
https://www.readbyqxmd.com/read/29382431/-identification-and-application-of-mycobacterium-tuberculosis-esxn-specific-cell-epitopes-in-the-diagnosis-of-pulmonary-tuberculosis
#11
Zhigang Xu, Dezhou Li, Xinyu Chen, Zhiliang Duan, Jiayuan Mao, Jinsheng Wen
Objective To identify Mycobacterium tuberculosis ESAT-6 protein esxN-specific HLA-A*0201-restricted CTL epitopes and assess the diagnostic potential of the identified epitopes in pulmonary tuberculosis. Methods The esxN-specific HLA-A*0201-restricted CTL epitopes were predicted by the T epitope prediction software SYFPEITHI and further synthesized. The binding affinity of the candidate epitopes for HLA-A*0201 was detected using MHC-peptide complex stabilization assay. The immunogenicity of candidate epitopes were assessed using ELISPOT in HLA-A*0201 transgenic mice...
December 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29371122/diagnostic-value-of-dual-input-computed-tomography-perfusion-on-detecting-bronchial-pulmonary-artery-fistula-in-tuberculosis-patients-with-massive-hemoptysis
#12
Haixian Qu, Maoqiang Wang, Zhijun Wang, Guokun Ao, Xiaodong Yuan, Qiang Li, Zepeng Ma, Qianru Xu, Jieyu Yan, Yanhua Bai
RATIONALE AND OBJECTIVES: This prospective study aimed to evaluate the diagnostic performance of dual-input computed tomography perfusion technique (DI-CTP) in identifying the bronchial-pulmonary artery fistula in patients tuberculosis with massive hemoptysis. MATERIAL AND METHODS: Twenty patients with tuberculosis with massive hemoptysis were enrolled from January 2015 to December 2015. The association between DI-CTP parameters and the diagnostic outcomes of digital subtraction angiography was assessed...
January 19, 2018: Academic Radiology
https://www.readbyqxmd.com/read/29368890/-diagnostic-rentability-of-close-pleural-biopsy-tru-cut-vs-cope
#13
Héctor Glenn Valdez-López, Alma Iris Cano-Rodríguez, Mario Montemayor-Chapa, Juan Francisco Castillo-Sánchez
BACKGROUND: The prevalence of pleural effusion in Mexico is over 400 per 100 000 inhabitants. The etiology is infectious in 45.7% and neoplastic in 32.6%. Closed pleural biopsy sensibility is 48-70% in cancer and 50-59% in tuberculosis using Cope or Abrams needle. In 1989, Tru-cut needle biopsy was described in a small study for massive pleural effusions with a sensibility of 86%. Our Institute has a wide experience with this infrequently procedure with reliable results than using Cope needle...
January 2018: Revista Médica del Instituto Mexicano del Seguro Social
https://www.readbyqxmd.com/read/29359690/from-latent-tuberculosis-infection-to-tuberculosis-news-in-diagnostics-quantiferon-plus
#14
Dagmara I Borkowska, Agnieszka M Napiórkowska, Sylwia A Brzezińska, Monika Kozińska, Anna T Zabost, Ewa M Augustynowicz-Kopeć
It is estimated that one third of the world's population have latent tuberculosis infection and that this is a significant reservoir for future tuberculosis cases. Most cases occur within two years following initial infection. The identification of individuals with latent tuberculosis infection is difficult due to the lack of an ideal diagnostic assay and incomplete understanding of latent infection. Currently, there are three tests: the oldest tuberculin skin test, T-SPOT.TB and the latest QuantiFERON-Plus for the detection of Mycobacterium tuberculosis infection...
March 30, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/29348383/improving-diagnostic-of-pulmonary-tuberculosis-in-hiv-patients-by-bronchoscopy-a-cross-sectional-study
#15
Prayudi Santoso, Arto Y Soeroto, Rianita Juniati, Yovita Hartantri, Rudi Wisaksana, Bachti Alisjabana, Heda M Nataprawira, Ida Parwati
BACKGROUND: diagnostic of pulmonary TB in HIV patients is a problem due to non specific clinical features, or radiological appearance. HIV patients with CD4≤200 cells/mL infected with M. tuberculosis have less capacity in containing M. tuberculosis, developing granulomas, casseous necrosis, or cavities. This condition is caused by weakend inflammatory which later reduced sputum production and may cause false negative result. This study aimed to assess differences in the positivity level of acid fast bacilli (AFB) and cultures of M...
October 2017: Acta Medica Indonesiana
https://www.readbyqxmd.com/read/29348166/clinical-evaluation-of-a-blood-assay-to-diagnose-paucibacillary-tuberculosis-via-bacterial-antigens
#16
Chang Liu, Christopher J Lyon, Yang Bu, Zaian Deng, Elisabetta Walters, Yan Li, Liqun Zhang, Anneke C Hesseling, Edward A Graviss, Ye Hu
BACKGROUND: The diagnosis of active tuberculosis (TB)10 cases primarily relies on methods that detect Mycobacterium tuberculosis (Mtb) bacilli or their DNA in patient samples (e.g., mycobacterial culture and Xpert MTB/RIF assays), but these tests have low clinical sensitivity for patients with paucibacillary TB disease. Our goal was to evaluate the clinical performance of a newly developed assay that can rapidly diagnose active TB cases by direct detection of Mtb-derived antigens in patients' blood samples...
January 18, 2018: Clinical Chemistry
https://www.readbyqxmd.com/read/29332654/evaluation-of-loop-mediated-isothermal-amplification-lamp-assay-in-the-diagnosis-of-tubercular-lymphadenitis-a-pilot-study
#17
Baijayantimala Mishra, Vinaykumar Hallur, Bijayini Behera, C Preetam, Priti Nanda Mishra, Jyotirmayee Turuk, Prasanta Raghab Mohapatra
Tubercular lymphadenitis (TBLA) contributes to 30-40% of extrapulmonary TB cases in the immunocompetent individuals and 40-50% in people with HIV. Current diagnostic methods for TBLA like Gene-Xpert or PCR are costly and conventional methods like fine needle aspiration cytology, histopathology lack sensitivity and specificity. Culture which is considered as gold standard require high turnaround time. Loop mediated isothermal amplification (LAMP) assay has been developed as a novel technique for nucleic acid amplification and has shown promising results in the diagnosis of pulmonary tuberculosis...
January 2018: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/29332644/role-of-serum-adenosine-deaminase-in-pulmonary-tuberculosis
#18
Varinder Saini, Bhaskar Lokhande, Shivani Jaswal, Deepak Aggarwal, Kranti Garg, Jasbinder Kaur
BACKGROUND: Definitive laboratory diagnosis and confirmation of tuberculosis remains a major challenge because of lack of specificity and sensitivity of diagnostic methods especially in sputum smear negative tuberculosis. Many studies have proved the role of ADA in diagnosis of tuberculosis in effusion fluids and a decrease in ADA activity after treatment. This study was aimed to investigate the role of serum ADA level as an early diagnostic and prognostic marker for pulmonary tuberculosis (PTB)...
January 2018: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/29330981/comparison-of-ziehl-neelsen-based-light-microscopy-with-led-fluorescent-microscopy-for-tuberculosis-diagnosis-an-insight-from-a-limited-resource-high-burden-setting
#19
Muhammad Yahya Noori, Faiza Ali, Zaheer Ali, Shaheen Sharafat
BACKGROUND: Microscopy is the most widely used tool for Tuberculosis screening. Conventionally, Ziehl-Neelsen (ZN) staining has been the widely used for staining Acid-Fast Bacilli (AFB) but with the advent of Fluorescent staining, Auramine O stain is now being adapted as the preferred method for setups with high workload as it has the advantage of being less laborious, since bacteria fluoresce in front of a dark background and are easier to count. This study was performed to compare the efficiency of the two methods in a high-burden, limited resource setting to see the magnitude of diagnostic accuracy between ZN and Fluorescent Microscopy, using culture as the standard...
October 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/29330384/detection-of-apparent-cell-free-m-tuberculosis-dna-from-plasma
#20
E S Click, W Murithi, G S Ouma, K McCarthy, M Willby, S Musau, H Alexander, E Pevzner, J Posey, K P Cain
New diagnostics are needed to improve clinicians' ability to detect tuberculosis (TB) disease in key populations such as children and persons living with HIV and to rapidly detect drug resistance. Circulating cell-free DNA (ccfDNA) in plasma is a diagnostic target in new obstetric and oncologic applications, but its utility for diagnosing TB is not known. Here we show that Mycobacterium tuberculosis complex DNA can be detected in plasma of persons with sputum smear-positive TB, even in the absence of mycobacteremia...
January 12, 2018: Scientific Reports
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