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diagnostic methods tuberculosis

Ambika Anand, Courtney Wagner, Steve S Kong, Elliot Griffith, Punnavit Harimtepathip, Kathryn K Baker, Stephen Rineer, Judith Simms-Cendan, Magdalena Pasarica
Introduction Latent tuberculosis infection (LTBI) screening with targeted treatment has been successful in eradicating tuberculosis (TB) as an endemic infection in the United States. The Centers for Disease Control and Prevention (CDC) recommends screening for high-risk patients. The aim of this study was to increase LTBI screening, detection, and treatment in our student-run free clinic while providing an innovative platform for education in primary care topics. Methods A questionnaire for screening for LTBI was adapted from CDC guidelines...
April 16, 2018: Curēus
Akul Singhania, Raman Verma, Christine M Graham, Jo Lee, Trang Tran, Matthew Richardson, Patrick Lecine, Philippe Leissner, Matthew P R Berry, Robert J Wilkinson, Karine Kaiser, Marc Rodrigue, Gerrit Woltmann, Pranabashis Haldar, Anne O'Garra
Whole blood transcriptional signatures distinguishing active tuberculosis patients from asymptomatic latently infected individuals exist. Consensus has not been achieved regarding the optimal reduced gene sets as diagnostic biomarkers that also achieve discrimination from other diseases. Here we show a blood transcriptional signature of active tuberculosis using RNA-Seq, confirming microarray results, that discriminates active tuberculosis from latently infected and healthy individuals, validating this signature in an independent cohort...
June 19, 2018: Nature Communications
Bernice J Klotoe, Barbara Molina-Moya, Harrison Magdinier Gomes, Michel K Gomgnimbou, Lorenna Oliveira Suzarte, Maria H Féres Saad, Sajid Ali, José Dominguez, Edita Pimkina, Elena Zholdybayeva, Christophe Sola, Guislaine Refrégier
Several diagnostic tests are being developed to detect drug resistance in tuberculosis. In line with previous developments detecting rifampicin and isoniazid resistance using microbead-based systems (spoligoriftyping and TB-SPRINT), we present here an assay called TB-EFI detecting mutations involved in resistance to ethambutol, fluoroquinolones and the three classical injectable drugs (kanamycin, amikacin and capreomycin) in Mycobacterium tuberculosis. The proposed test includes both wild-type and mutant probes for each targeted locus...
June 15, 2018: Journal of Microbiological Methods
Tatiana Metcalf, Jaime Soria, Silvia M Montano, Eduardo Ticona, Carlton A Evans, Luz Huaroto, Matthew Kasper, Eric S Ramos, Nicanor Mori, Podjanee Jittamala, Kesinee Chotivanich, Irwin F Chavez, Pratap Singhasivanon, Sasithon Pukrittayakamee, Joseph R Zunt
BACKGROUND: Meningitis caused by Mycobacterium tuberculosis is a major cause of morbidity and mortality worldwide. We evaluated the performance of cerebrospinal fluid (CSF) testing with the GeneXpert MTB/RIF assay versus traditional approaches for diagnosing tuberculosis meningitis (TBM). METHODS: Patients were adults (n = 37) presenting with suspected TBM to the Hospital Nacional Dos de Mayo, Lima, Peru, during 12 months until 1st January 2015. Each participant had a single CSF specimen that was divided into aliquots that were concurrently tested for M...
2018: PloS One
Ronaldo Rodrigues da Costa, Suzane Fernandes da Silva, Romário Costa Fochat, Raquel Leite Macedo, Thamiris Vilela Pereira, Marcio Roberto Silva, Carmen Perches Gomide Pinto, Isabel Cristina Gonçalves Leite
OBJECTIVE: To compare the performance of the Ogawa-Kudoh method with the modified Petroff technique in diagnosis of pulmonary tuberculosis. METHODS: A total of 205 sputum samples from 166 patients with clinical suspicion or under pulmonary tuberculosis follow-up, seen at a public tertiary care hospital, from July 2014 to July 2016 were used. All samples were simultaneously processed using the Ogawa-Kudoh and modified Petroff decontamination methods, according to the recommendations of the Ministry of Health...
June 11, 2018: Einstein
Mahmut Sunnetcioglu, Ali Irfan Baran, Irfan Binici, Fatih Esmer, Bilge Gultepe
OBJECTIVE: To determine whether there were any changes in demographic and clinical features of extrapulmonary tuberculosis cases. METHODS: This retrospective study was conducted at Yuzuncu Yil University, Van, Turkey, and comprised records of extrapulmonary tuberculosis patients treated between January 2009 and July 2014 at the tuberculosis control dispensary. Descriptive and clinical data, including age, gender, site of involvement, diagnostic method and coexisting systemic diseases, were noted...
May 2018: JPMA. the Journal of the Pakistan Medical Association
Ying Kong, Jeffrey D Cirillo
Fluorescence imaging has been applied to various areas of biological research, including studies of physiological, neurological, oncological, cell biological, molecular, developmental, immunological, and infectious processes. In this chapter, we describe methods of fluorescent imaging applied to examination of subcutaneous and pulmonary mycobacterial infections in an animal model. Since slow growth of Mycobacterium tuberculosis (Mtb) hinders development of new diagnostics, therapeutics, and vaccines for tuberculosis (TB), we developed fluorescent protein (FP) expressing mycobacterial strains for in vivo imaging, which can be used to track bacterial location and to quantitate bacterial load directly in living animals...
2018: Methods in Molecular Biology
Ronan M Doyle, Carrie Burgess, Rachel Williams, Rebecca Gorton, Helen Booth, James Brown, Josephine M Bryant, Jackie Chan, Dean Creer, Jolyon Holdstock, Heinke Kunst, Stefan Lozewicz, Gareth Platt, Erika Yara Romero, Graham Speight, Simon Tiberi, Ibrahim Abubakar, Marc Lipman, Timothy D McHugh, Judith Breuer
The current methods available to diagnose antimicrobial resistant Mycobacterium tuberculosis infections require positive culture or only test a limited number of resistance-associated mutations. Rapid, accurate identification of antimicrobial resistance enables prompt initiation of effective treatment. Here, we determine the utility of whole-genome sequencing (WGS) M. tuberculosis directly from routinely obtained diagnostic sputum samples to provide a comprehensive resistance profile compared to Mycobacterial Growth Indicator Tube (MGIT) WGS...
May 30, 2018: Journal of Clinical Microbiology
P M Gordaliza, A Muñoz-Barrutia, L E Via, S Sharpe, M Desco, J J Vaquero
PURPOSE: Computed tomography (CT) images enable capturing specific manifestations of tuberculosis (TB) that are undetectable using common diagnostic tests, which suffer from limited specificity. In this study, we aimed to automatically quantify the burden of Mycobacterium tuberculosis (Mtb) using biomarkers extracted from x-ray CT images. PROCEDURES: Nine macaques were aerosol-infected with Mtb and treated with various antibiotic cocktails. Chest CT scans were acquired in all animals at specific times independently of disease progression...
May 29, 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Panuvat Chutichetpong, Nopchulee Cheeveewattanagul, Prapaporn Srilohasin, Patsamon Rijiravanich, Angkana Chaiprasert, Werasak Surareungchai
Drug susceptibility testing (DST) for Mycobacterium tuberculosis currently faces multiple challenges, including lengthy ineffective standard methods, the expensive cost of molecular tests, and large bulky diagnostic machines. In this work, a disposable MPT64 sensor was developed to rapidly determine drug susceptibility (DS-TB) and multidrug resistant tuberculosis (MDR-TB) using an electrochemical sandwich-immunosensor for the detection of MPT64 as an indicator of Mycobacterium tuberculosis growth. Anti-MPT64 (as capture antibody; cAb) was immobilised on screen printed carbon electrodes to specifically bind with MPT64 target protein...
September 26, 2018: Analytica Chimica Acta
Sonia Ahlawat, Surekha Dabla, Vinay Kumar, Machiavelli Singh, Kiran Bala, Promod K Mehta
BACKGROUND Tuberculoma and neurocysticercosis (NCC) often show similar clinical and neuroimaging features. Differential diagnosis of these 2 diseases is imperative, as tuberculoma is an active infection that requires immediate anti-tubercular therapy (ATT). CASE REPORT We present the case of a 17-year-old Indian girl with fever, severe headache, and right 6th cranial nerve palsy. Brain magnetic resonance imaging (MRI) showed multiple tiny ring-enhancing lesions in bilateral cerebral parenchyma with mild perilesional edema, which were initially thought to be NCC, but subsequently were diagnosed as brain tuberculomas...
May 24, 2018: American Journal of Case Reports
Kiran Thakur, Mitashee Das, Kelly E Dooley, Amita Gupta
Central nervous system (CNS) involvement of tuberculosis (TB) is the most severe manifestation of TB and accounts for approximately 5 to 10% of all extrapulmonary TB (EPTB) cases and approximately 1% of all TB cases. TB meningitis (TBM) is the most common form of CNS TB, though other forms occur, often in conjunction with TBM, including intracranial tuberculomas, tuberculous brain abscesses, and spinal tubercular arachnoiditis. CNS TB often presents with nonspecific clinical features that mimic symptoms of other neurological conditions, often making diagnosis difficult...
April 2018: Seminars in Neurology
Davood Azadi, Tahereh Motallebirad, Kazem Ghaffari, Hasan Shojaei
Background: Tuberculosis is one of the most important infectious diseases that has claimed its victims throughout much of known human history. With Koch's discovery of the tubercle bacillus as the etiologic agent of the disease, his sanitary and hygienic measures, which were based on his discovery and the development of a vaccine against tuberculosis by Albert Calmette and Camille Guérin in 1921, an attenuated Mycobacterium bovis strain, bacilli Calmette-Guérin (BCG), and the discovery of the first antibiotic against tuberculosis, streptomycin by Selman Waksman in 1943, soon led to the opinion that appropriate control measures had become available for tuberculosis and it had been assumed that the disease could ultimately be eradicated...
2018: Open Microbiology Journal
Shagun Gupta, Vipan Kakkar
Early diagnosis and on-time effective treatment are indispensable for Tuberculosis (TB) control - a life threatening infectious communicable disease. The conventional techniques for diagnosing TB normally take two to three weeks. This delay in diagnosis and further increase in detection complexity due to the emerging risks of XDR-TB (Extensively drug Resistant-TB) and MDR-TB (Multidrug Resistant-TB) are evoking interest of researchers in the field of developing rapid TB detection techniques such as biosensing and other point-of-care (POC) techniques...
September 15, 2018: Biosensors & Bioelectronics
Jing-Wen Ai, Yang Li, Qi Cheng, Peng Cui, Hong-Long Wu, Bin Xu, Wen-Hong Zhang
BACKGROUND: A 45-year-old man who complained of continuous fever and multiple hepatic masses was admitted to our hospital. Repeated MRI manifestations were similar while each radiological report suggested contradictory diagnosis pointing to infections or malignances respectively. Pathologic examination of the liver tissue showed no direct evidence of either infections or tumor. We performed next-generation sequencing on the liver tissue and peripheral blood to further investigate the possible etiology...
May 11, 2018: Clinics and Research in Hepatology and Gastroenterology
Melissa Davidsen Jørstad, Msafiri Marijani, Anne Ma Dyrhol-Riise, Lisbet Sviland, Tehmina Mustafa
BACKGROUND: Extrapulmonary tuberculosis (EPTB) is a diagnostic challenge. An immunochemistry-based MPT64 antigen detection test (MPT64 test) has reported higher sensitivity in the diagnosis of EPTB compared with conventional methods. The objective of this study was to implement and evaluate the MPT64 test in routine diagnostics in a low-resource setting. METHODS: Patients with presumptive EPTB were prospectively enrolled at Mnazi Mmoja Hospital, Zanzibar, and followed to the end of treatment...
2018: PloS One
Kursat Arslan, Ufuk Taner, Suleyman Cagan Efe, Burak Ayca, Turgut Karabag
Tuberculosis-associated pericardial disorders are an excessively rare manifestation of extrapulmonary tuberculosis. The patients may present with constrictive pericarditis or pericardial fluid accumulation leading to cardiac tamponade. This paper reports a case of tuberculosis-associated pericardial effusion with dense fibrinous material not causing tamponade in a foreigner presenting with nonspecific symptoms. It also provides a discussion about the diagnostic and therapeutic methods as well as interesting echocardiographic images of the patient...
May 2, 2018: Medical Ultrasonography
Malesiba Mampotoko Selepe, Abraham Christoffel van Wyk, Kim Gilberte Pauline Hoek, Yvonne Prince, Paul David van Helden, Robin Mark Warren, Colleen Anne Wright
BACKGROUND: The rapid diagnosis of extrapulmonary tuberculosis in children remains challenging. The presence of enlarged lymph nodes provides an opportunity to obtain diagnostic material through fine needle aspiration biopsy (FNAB). Mycobacterial culture, traditionally the reference standard, has a slow turnaround time and PCR-based methods are not widely available in developing countries. Direct visualization of mycobacteria on microscopy can be a rapid method to confirm the diagnosis...
May 3, 2018: Diagnostic Cytopathology
Hansong Chae, Sung Jae Shin
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a serious global health problem in the 21st century because of its high mortality. Mtb is an extremely successful human-adapted pathogen that displays a multifactorial ability to control the host immune response and to evade killing by drugs, resulting in the breakdown of BCG vaccine-conferred anti-TB immunity and development of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mtb. Although genetic components of the genomes of the Mtb complex strains are highly conserved, showing over 99% similarity to other bacterial genera, recently accumulated evidence suggests that the genetic diversity of the Mtb complex strains has implications for treatment outcomes, development of MDR/XDR Mtb, BCG vaccine efficacy, transmissibility, and epidemiological outbreaks...
May 2018: Journal of Microbiology / the Microbiological Society of Korea
Y M Park, K H Oh, J-G Cho, S-K Baek, S-Y Kwon, K-Y Jung, J-S Woo
In this study, we compared the diagnostic accuracy and safety of fine-needle aspiration cytology and core-needle biopsy in patients with cervical lymphadenopathy or salivary gland tumour, and provided a basis for selecting the appropriate diagnostic method in clinical situations. A total of 278 patients were included in this study. The sensitivities of fine-needle aspiration cytology and core-needle biopsy were 66.7% and 100%, respectively, and negative predictive values were 92.6% and 100%, respectively, for diagnosing malignancy...
April 27, 2018: International Journal of Oral and Maxillofacial Surgery
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