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https://www.readbyqxmd.com/read/29454017/a-rapid-method-for-titration-of-ascovirus-infectivity
#1
Ningning Han, Zishu Chen, Hu Wan, Guohua Huang, Jianhong Li, Byung Rae Jin
Ascoviruses are a recently described family and the traditional plaque assay and end-point PCR assay have been used for their titration. However, these two methods are time-consuming and inaccurate to titrate ascoviruses. In the present study, a quick method for the determination of the titer of ascovirus stocks was developed based on ascovirus-induced apoptosis in infected insect cells. Briefly, cells infected with serial dilutions of virus (10 -2 to 10 -10 ) for 24 h were stained with trypan blue. The stained cells were counted, and the percentage of nonviable cells was calculated...
February 14, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29453683/flow-energy-loss-as-a-predictive-parameter-for-right-ventricular-deterioration-caused-by-pulmonary-regurgitation-after-tetralogy-of-fallot-repair
#2
Miyuki Shibata, Keiichi Itatani, Taiyu Hayashi, Takashi Honda, Atsushi Kitagawa, Kagami Miyaji, Minoru Ono
The optimal timing for pulmonary valve replacement after Tetralogy of Fallot (TOF) repair remains controversial. In this study, we estimated the feasibility of using flow energy loss (FEL) to predict right ventricular (RV) deterioration due to pulmonary regurgitation after TOF repair. We examined RV outflow tract (RVOT) flow in nine patients who underwent TOF or double-outlet right ventricle repair in the intervention group (Group I) and compared them with three healthy children in the control group (Group C)...
February 16, 2018: Pediatric Cardiology
https://www.readbyqxmd.com/read/29453333/scleraxis-is-a-transcriptional-activator-that-regulates-the-expression-of-tenomodulin-a-marker-of-mature-tenocytes-and-ligamentocytes
#3
Chisa Shukunami, Aki Takimoto, Yuriko Nishizaki, Yuki Yoshimoto, Seima Tanaka, Shigenori Miura, Hitomi Watanabe, Tetsushi Sakuma, Takashi Yamamoto, Gen Kondoh, Yuji Hiraki
Tenomodulin (Tnmd) is a type II transmembrane glycoprotein predominantly expressed in tendons and ligaments. We found that scleraxis (Scx), a member of the Twist-family of basic helix-loop-helix transcription factors, is a transcriptional activator of Tnmd expression in tenocytes. During embryonic development, Scx expression preceded that of Tnmd. Tnmd expression was nearly absent in tendons and ligaments of Scx-deficient mice generated by transcription activator-like effector nucleases-mediated gene disruption...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453313/multigene-profiling-of-ctcs-in-mcrpc-identifies-a-clinically-relevant-prognostic-signature
#4
Udit Singhal, Yugang Wang, James Henderson, Yashar S Niknafs, Yuanyuan Qiao, Amy Gursky, Alexander Zaslavsky, Jae-Seung Chung, David C Smith, R Jeffrey Karnes, S Laura Chang, Felix Y Feng, Ganesh S Palapattu, Russell S Taichman, Arul M Chinnaiyan, Scott A Tomlins, Todd M Morgan
The trend towards precision-based therapeutic approaches dictated by molecular alterations offers substantial promise for men with metastatic castration resistant prostate cancer (mCRPC). However, current approaches for molecular characterization are primarily tissue based, necessitating serial biopsies to understand changes over time and are limited by the challenges inherent to extracting genomic material from predominantly bone metastases. Therefore, a circulating tumor cell (CTC)-based assay was developed to determine gene expression across a panel of clinically relevant and potentially actionable prostate cancer related genes...
February 16, 2018: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29453278/molecular-signatures-of-circulating-melanoma-cells-for-monitoring-early-response-to-immune-checkpoint-therapy
#5
Xin Hong, Ryan J Sullivan, Mark Kalinich, Tanya Todorova Kwan, Anita Giobbie-Hurder, Shiwei Pan, Joseph A LiCausi, John D Milner, Linda T Nieman, Ben S Wittner, Uyen Ho, Tianqi Chen, Ravi Kapur, Donald P Lawrence, Keith T Flaherty, Lecia V Sequist, Sridhar Ramaswamy, David T Miyamoto, Michael Lawrence, Mehmet Toner, Kurt J Isselbacher, Shyamala Maheswaran, Daniel A Haber
A subset of patients with metastatic melanoma have sustained remissions following treatment with immune checkpoint inhibitors. However, analyses of pretreatment tumor biopsies for markers predictive of response, including PD-1 ligand (PD-L1) expression and mutational burden, are insufficiently precise to guide treatment selection, and clinical radiographic evidence of response on therapy may be delayed, leading to some patients receiving potentially ineffective but toxic therapy. Here, we developed a molecular signature of melanoma circulating tumor cells (CTCs) to quantify early tumor response using blood-based monitoring...
February 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29453249/hhex-induces-promyelocyte-self-renewal-and-cooperates-with-growth-factor-independence-to-cause-myeloid-leukemia-in-mice
#6
Jacob T Jackson, Ashley P Ng, Benjamin J Shields, Sue Haupt, Ygal Haupt, Matthew P McCormack
The hematopoietically expressed homeobox (Hhex) transcription factor is overexpressed in human myeloid leukemias. Conditional knockout models of murine acute myeloid leukemia indicate that Hhex maintains leukemia stem cell self-renewal by enabling Polycomb-mediated epigenetic repression of the Cdkn2a tumor suppressor locus, encoding p16 Ink4a and p19 Arf However, whether Hhex overexpression also affects hematopoietic differentiation is unknown. To study this, we retrovirally overexpressed Hhex in hematopoietic progenitors...
February 27, 2018: Blood Advances
https://www.readbyqxmd.com/read/29452461/angiogenesis-precedes-cardiomyocyte-migration-in-regenerating-mammalian-hearts
#7
Arnar B Ingason, Andrew B Goldstone, Michael J Paulsen, Akshara D Thakore, Vi N Truong, Bryan B Edwards, Anahita Eskandari, Tanner Bollig, Amanda N Steele, Y Joseph Woo
OBJECTIVE: Although the mammalian heart's ability to fully regenerate is debated, its potential to extensively repair itself is gaining support. We hypothesized that heart regeneration relies on rapid angiogenesis to support myocardial regrowth and sought to characterize the timeline for angiogenesis and cell proliferation in regeneration. METHODS: One-day-old CD-1 mice (P1, N = 60) underwent apical resection or sham surgery. Hearts were explanted at serial time points from 0 to 30 days postresection and analyzed with immunohistochemistry to visualize vessel ingrowth and cardiomyocyte migration into the resected region...
March 2018: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29452310/serial-long-term-follow-up-of-a-medically-managed-aortic-prosthetic-valve-thrombosis
#8
Bonou Maria, Tzanis Georgios, Viniou Nora-Athina, Biliou Smaragda, Barbetseas John
No abstract text is available yet for this article.
February 13, 2018: Hellenic Journal of Cardiology: HJC, Hellēnikē Kardiologikē Epitheōrēsē
https://www.readbyqxmd.com/read/29452203/morphology-of-the-elytral-base-sclerites
#9
Miwa Sugimoto, Naoki Ogawa, Kazunori Yoshizawa
The elytral base sclerites (= sclerites located at the articular region between the forewing and thorax in Coleoptera) of selected taxa were examined and homologized. Although the elytral base sclerites are highly modified compared to the wing base sclerites of the other neopterans, they can be homologized by using the conservative wing flapping and folding lines as landmarks. A reduction of the first axillary sclerite was identified as a general trend of the elytral base sclerites, although the sclerite usually has a very important function to mediate flight power from the notum to the wing...
February 13, 2018: Arthropod Structure & Development
https://www.readbyqxmd.com/read/29451816/effects-of-teriparatide-on-morphology-of-aortic-calcification-in-aged-hyperlipidemic-mice
#10
Jeffrey J Hsu, Jinxiu Lu, Soban Umar, Jason T Lee, Rajan Kulkarni, Yichen Ding, Chih-Chiang Chang, Tzung K Hsiai, Akishige Hokugo, Ioannis Gkouveris, Sotirios Tetradis, Ichiro Nishimura, Linda L Demer, Yin Tintut
Calcific aortic vasculopathy correlates with bone loss in osteoporosis in an age-independent manner. Prior work suggests that teriparatide, the bone anabolic treatment for postmenopausal osteoporosis, may inhibit onset of aortic calcification. Whether teriparatide affects progression of pre-existing aortic calcification, widespread among this patient population, is unknown. Female apolipoprotein E-deficient mice were aged for over one year to induce aortic calcification, treated for 4.5 weeks with daily injections of control vehicle (PBS), teriparatide 40 µg/kg (PTH40) or 400 µg/kg (PTH400), and assayed for aortic calcification by micro-computed tomography (microCT) before and after treatment...
February 16, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29451445/repeated-measurements-of-renal-function-in-evaluating-its-decline-in-cats
#11
Natalie C Finch, Harriet M Syme, Jonathan Elliott
Objectives The aim of this study was to describe the variability in renal function markers in non-azotaemic and azotaemic cats, and also the rate of change in the markers. Methods Plasma creatinine concentration and its reciprocal, glomerular filtration rate (GFR) and urine specific gravity (USG) were studied as markers of renal function in client-owned cats. GFR was determined using a corrected slope-intercept iohexol clearance method. Renal function testing was performed at baseline and a second time point...
February 1, 2018: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/29450869/long-term-observation-of-acute-onset-autoimmune-hepatitis-presenting-clinically-and-radiologically-as-acute-hepatitis
#12
Keiichi Fujiwara, Yoshihiro Fukuda, Katsushi Seza, Masaya Saito, Shin Yasui, Masayuki Nakano, Osamu Yokosuka, Naoya Kato
BACKGROUND: There is yet no gold standard for the diagnosis of acute-onset autoimmune hepatitis (A-AIH), especially histologically acute AIH. As a result, long-term observation of A-AIH has been difficult and the nature is not well known. We retrospectively analyzed the clinicopathological features of A-AIH over a long prospective follow-up period. METHODS: Clinical, biochemical, immunological and pathological features of 30 patients (21 female, mean age 55.1 ± 13...
February 15, 2018: Hepatology International
https://www.readbyqxmd.com/read/29450743/construction-of-a-reverse-genetic-system-for-porcine-astrovirus
#13
Yifeng Qin, Qingli Fang, Huan Liu, Chengyuan Ji, Ying Chen, Kang Ouyang, Zuzhang Wei, Weijian Huang
In order to construct a full-length infectious cDNA clone of porcine astrovirus, three fragments covering the complete genome of PAstV1-GX1 strain were amplified by RT-PCR. All three PCR-amplified fragments were cloned into T-Vector pMD19 (Simple), and subsequently assembled into a full-length cDNA clone by subcloning. A silent nucleotide change creating a PstI site was engineered into the full-length cDNA clone to distinguish the rescued virus from the parental virus. Upon transfection of BHK-21 cells with the in vitro transcripts of both the original and constructed cDNAs, typical cytopathic effects were observed on PK-15 cells after serial passaging of the cell supernatant...
February 15, 2018: Archives of Virology
https://www.readbyqxmd.com/read/29450721/analysis-of-changes-in-thermal-growing-season-indices-tgsi-and-their-relations-with-some-selected-atmospheric-teleconnection-patterns-atps-over-the-northwest-of-iran
#14
Ali Akbar Sabziparvar, Fatemeh Khoshhal Jahromi
The daily minimum air temperature data from 18 stations located in the northwest of Iran during the period 1986-2015 was used to analyse the inter-annual variations and trends of thermal growing season indices (tGSI) and their relations with different atmospheric teleconnection patterns (ATPs). To analyze the changes in tGSI, tGSS (thermal growing season start), tGSE (thermal growing season end), and tGSL, the time period between tGSS and tGSE were considered. Using non-parametric Mann-Kendall and Spearman tests, the existence of a significant trend for time series of the tGSI and the correlation between ATPs and tGSI was evaluated...
February 15, 2018: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/29450606/combination-transarterial-chemoembolization-and-microwave-ablation-improves-local-tumor-control-for-3-to-5-cm-hepatocellular-carcinoma-when-compared-with-transarterial-chemoembolization-alone
#15
Amanda R Smolock, Mircea M Cristescu, Audrey Hinshaw, Kaitlin M Woo, Shane A Wells, Timothy J Ziemlewicz, Meghan G Lubner, Prasad S Dalvie, J Louis Hinshaw, Christopher L Brace, Orhan S Ozkan, Fred T Lee, Paul Laeseke
PURPOSE: To compare transarterial chemoembolization (TACE) monotherapy to combination TACE and microwave ablation (MWA) for local control of 3- to 5-cm hepatocellular carcinoma (HCC). METHODS: Patients with HCC between 3 and 5 cm treated with TACE monotherapy or combination TACE + MWA at a single institution between 2007 and 2016 were retrospectively reviewed. Twenty-four HCCs (median diameter 3.8 cm) in 16 patients (13 males; median age 64 years) were treated using TACE monotherapy...
February 15, 2018: Abdominal Radiology
https://www.readbyqxmd.com/read/29450470/incidence-of-delayed-intracranial-hemorrhage-in-older-patients-after-blunt-head-trauma
#16
James A Chenoweth, Samuel D Gaona, Mark Faul, James F Holmes, Daniel K Nishijima
Importance: Current guidelines conflict on the management of older adults who have blunt head trauma taking anticoagulant and antiplatelet medications. This is partially due to the limited data comparing patients who are taking these medications with those who are not. Objective: To investigate the incidence of delayed traumatic intracranial hemorrhage in older adults with head trauma, including those taking anticoagulant and antiplatelet medications. Design, Setting, and Participants: This prospective observational cohort study included patients 55 years and older who had blunt head trauma and were transported via emergency medical services between August 1, 2015, and September 30, 2016...
February 14, 2018: JAMA Surgery
https://www.readbyqxmd.com/read/29449271/targeted-therapies-for-targeted-populations-anti-egfr-treatment-for-egfr-amplified-gastroesophageal-adenocarcinoma
#17
Steven B Maron, Lindsay Alpert, Heewon A Kwak, Samantha Lomnicki, Leah Chase, David Xu, Emily O'Day, Rebecca J Nagy, Richard B Lanman, Fabiola Cecchi, Todd Hembrough, Alexa Schrock, John Hart, Shu-Yuan Xiao, Namrata Setia, Daniel V T Catenacci
Previous anti-EGFR trials in unselected gastroesophageal adenocarcinoma (GEA) patients were resoundingly negative. We identified EGFR amplification in 5% (19/363) of patients at the University of Chicago, including 6% (8/140) who were prospectively screened with intention-to-treat using anti-EGFR therapy. Seven pts received >1 dose of treatment: three first line FOLFOX plus ABT-806, one second line FOLFIRI plus cetuximab, and three third/fourth line cetuximab alone. Treatment achieved objective response in 58% (4/7) and disease control in 100% (7/7) with a median progression-free survival of 10 months...
February 15, 2018: Cancer Discovery
https://www.readbyqxmd.com/read/29448889/potential-of-the-moringa-oleifera-saline-extract-for-the-treatment-of-dairy-wastewater-application-of-the-response-surface-methodology
#18
Dalila Maria Formentini-Schmitt, Márcia Regina Fagundes-Klen, Márcia Teresinha Veit, Soraya Moreno Palácio, Daniela Estelita Goes Trigueros, Rosangela Bergamasco, Gustavo Affonso Pisano Mateus
In this work, the coagulation/flocculation/sedimentation treatment of dairy wastewater samples was investigated through serial factorial designs utilizing the saline extract obtained from Moringa oleifera (Moringa) as a coagulant. The sedimentation time, pH, Moringa coagulant (MC) dose and concentration of CaCl 2 have been evaluated through the response surface methodology (RSM) in order to obtain the ideal turbidity removal conditions. The empirical quadratic model, in conjunction with the desirability function, demonstrated that it is possible to obtain turbidity removals of 98...
February 16, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29448503/construction-and-characterization-of-cell-penetrating-peptide-fused-fibroblast-growth-factor-and-vascular-endothelial-growth-factor-for-an-enhanced-percutaneous-delivery-system
#19
Sang Won Choi, Rudra Pangeni, Dai Hyun Jung, Su Jung Kim, Jin Woo Park
The exogenous administration of growth factors has been examined for treating wounds and such factors serve as cosmetic agents for skin regeneration. However, the topical application of growth factors is hampered by their limited percutaneous absorption. In this study, we genetically modified basic fibroblast growth factor (bFGF) and vascular endothelial growth factor-A (VEGF-A) using a cell-penetrating peptide to facilitate their permeation into skin and to avoid multiple steps in the chemical or physical modification of biomaterials...
February 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29448186/cognitive-development-in-children-of-adolescent-mothers-the-impact-of-socioeconomic-risk-and-maternal-sensitivity
#20
Christine Firk, Kerstin Konrad, Beate Herpertz-Dahlmann, Wolfgang Scharke, Brigitte Dahmen
BACKGROUND: Adolescent motherhood is accompanied by a constellation of risk factors that translate into developmental risk for the off-spring. Socioeconomic risk that is associated with adolescent motherhood as well as maternal interactive behaviors may contribute to the impact of adolescent motherhood on children's developmental outcome. OBJECTIVE: Therefore, the aim of the current study was to investigate differences in children's cognitive development between children of adolescent and adult mothers in their first two years of life and to examine whether socioeconomic risk (e...
February 12, 2018: Infant Behavior & Development
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