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Canine, veterinary, regenerative medicine

Hyeok-Soo Shin, Heung-Myong Woo, Byung-Jae Kang
BACKGROUND: Platelet-rich plasma (PRP) has been expected for regenerative medicine because of its growth factors. However, there is considerable variability in the recovery and yield of platelets and the concentration of growth factors in PRP preparations. The aim of this study was to identify optimal relative centrifugal force and spin time for the preparation of PRP from canine blood using a double-centrifugation tube method. METHODS: Whole blood samples were collected in citrate blood collection tubes from 12 healthy beagles...
June 26, 2017: BMC Veterinary Research
Toshiya Nishimura, Shingo Hatoya, Ryoji Kanegi, Daluthgamage Pasty Himali Wijesekera, Kousuke Sanno, Erina Tanaka, Kikuya Sugiura, Noritoshi Kawate Hiromitsu Tamada, Hiroshi Imai, Toshio Inaba
Canine induced pluripotent stem cells (ciPSCs) are an attractive source for regenerative veterinary medicine, and may also serve as a disease model for human regenerative medicine. Extending the application of ciPSCs from bench to bedside, however, requires resolving many issues. We generated ciPSCs expressing doxycycline-inducible murine Oct3/4 (Pou5f1), Sox2, Klf4, and c-Myc, which were introduced using lentiviral vectors. The resultant ciPSCs required doxycycline to proliferate in the undifferentiated state...
April 2017: Molecular Reproduction and Development
Cheryl Stroud, Igor Dmitriev, Elena Kashentseva, Jeffrey N Bryan, David T Curiel, Hans Rindt, Carol Reinero, Carolyn J Henry, Philip J Bergman, Nicola J Mason, Josephine S Gnanandarajah, Julie B Engiles, Falon Gray, Danielle Laughlin, Anita Gaurnier-Hausser, Anu Wallecha, Margie Huebner, Yvonne Paterson, Daniel O'Connor, Laura S Treml, James P Stannard, James L Cook, Marc Jacobs, Gerald J Wyckoff, Lee Likins, Ubadah Sabbagh, Andrew Skaff, Amado S Guloy, Harlen D Hays, Amy K LeBlanc, Joan R Coates, Martin L Katz, Leslie A Lyons, Gayle C Johnson, Gary S Johnson, Dennis P O'Brien, Dongsheng Duan, James P Calvet, Barbara Gandolfi, David A Baron, Mark L Weiss, Debra A Webster, Francis N Karanu, Edward J Robb, Robert J Harman
A1 One health advances and successes in comparative medicine and translational researchCheryl StroudA2 Dendritic cell-targeted gorilla adenoviral vector for cancer vaccination for canine melanomaIgor Dmitriev, Elena Kashentseva, Jeffrey N. Bryan, David T. CurielA3 Viroimmunotherapy for malignant melanoma in the companion dog modelJeffrey N. Bryan, David Curiel, Igor Dmitriev, Elena Kashentseva, Hans Rindt, Carol Reinero, Carolyn J. HenryA4 Of mice and men (and dogs!): development of a commercially licensed xenogeneic DNA vaccine for companion animals with malignant melanomaPhilip J...
August 2016: Clinical and Translational Medicine
Nathalie Saulnier, Julia Loriau, Marine Febre, Clément Robert, Rodolphe Rakic, Tancrède Bonte, Samuel Buff, Stéphane Maddens
In veterinary medicine, therapeutic mesenchymal stromal cells (MSC) have been traditionally isolated from adult bone marrow or adipose tissue. Neonatal tissues, normally discarded at birth from all species have become an alternative source of cells for regenerative medicine in the human clinic. These cells have been described as being more primitive, proliferative and immunosuppressive than their adult counterparts. Our objective was to examine if this phenomena holds true in dogs. Little information exists regarding canine neonatal MSC characterisation...
March 2016: Veterinary Immunology and Immunopathology
Camila Marx, Maiele Dornelles Silveira, Nance Beyer Nardi
Mesenchymal stem cells, considered one of the most promising cell types for therapeutic applications due to their capacity to secrete regenerative bioactive molecules, are present in all tissues. Stem cells derived from the adipose tissue have been increasingly used for cell therapy in humans and animals, both as freshly isolated, stromal vascular fraction (SVF) cells, or as cultivated adipose-derived stem cells (ASCs). ASCs have been characterized in different animal species for proliferation, differentiation potential, immunophenotype, gene expression, and potential for tissue engineering...
April 1, 2015: Stem Cells and Development
Barbara G McMahill, Dori L Borjesson, Maya Sieber-Blum, Jan A Nolta, Beverly K Sturges
The use of cell transplantation for spinal cord injury is a rapidly evolving field in regenerative medicine. Numerous animal models are currently being used. However, translation to human patients is still a challenging step. Dogs are of increasing importance as a translational model for human disease since there is a greater awareness of the need to increase the quality of preclinical data. The use of dogs ultimately brings benefit to both human and veterinary medicine. In this review we analyze experimental and clinical studies using cell transplantation for canine spinal cord injury...
February 2015: Stem Cell Reviews
Francisco Javier Rodríguez-Jiménez, Teresa Valdes-Sánchez, José M Carrillo, Mónica Rubio, Manuel Monleon-Prades, Dunia Mercedes García-Cruz, Montserrat García, Ramón Cugat, Victoria Moreno-Manzano
Osteoarticular pathologies very often require an implementation therapy to favor regeneration processes of bone, cartilage and/or tendons. Clinical approaches performed on osteoarticular complications in dogs constitute an ideal model for human clinical translational applications. The adipose-derived mesenchymal stem cells (ASCs) have already been used to accelerate and facilitate the regenerative process. ASCs can be maintained in vitro and they can be differentiated to osteocytes or chondrocytes offering a good tool for cell replacement therapies in human and veterinary medicine...
2012: Journal of Functional Biomaterials
Hedwig S Kruitwagen, Bart Spee, Baukje A Schotanus
New curative therapies for severe liver disease are urgently needed in both the human and veterinary clinic. It is important to find new treatment modalities which aim to compensate for the loss of parenchymal tissue and to repopulate the liver with healthy hepatocytes. A prime focus in regenerative medicine of the liver is the use of adult liver stem cells, or hepatic progenitor cells (HPCs), for functional recovery of liver disease. This review describes recent developments in HPC research in dog and cat and compares these findings to experimental rodent studies and human pathology...
2014: BMC Veterinary Research
Baukje A Schotanus, Louis C Penning, Bart Spee
Liver cell turnover is very slow, especially compared to intestines and stomach epithelium and hair cells. Since the liver is the main detoxifying organ in the body, it does not come as a surprise that the liver has an unmatched regenerative capacity. After 70% partial hepatectomy, the liver size returns to normal in about two weeks due to replication of differentiated hepatocytes and cholangiocytes. Despite this, liver diseases are regularly encountered in the veterinary clinic. Dogs primarily present with parenchymal pathologies such as hepatitis...
December 2013: Veterinary Quarterly
Sang-Bum Park, Min-Soo Seo, Hyung-Sik Kim, Kyung-Sun Kang
Recent studies have shown that amniotic membrane tissue is a rich source of stem cells in humans. In clinical applications, the amniotic membrane tissue had therapeutic effects on wound healing and corneal surface reconstruction. Here, we successfully isolated and identified multipotent stem cells (MSCs) from canine amniotic membrane tissue. We cultured the canine amniotic membrane-derived multipotent stem cells (cAM-MSCs) in low glucose DMEM medium. cAM-MSCs have a fibroblast-like shape and adhere to tissue culture plastic...
2012: PloS One
Susan W Volk, Yanjian Wang, Kurt D Hankenson
Clinical trials utilizing bone marrow-derived mesenchymal stem cell (BM-MSC) therapies show promise for treating a variety of pathologic conditions. Paramount to optimization of such cell-based therapies is a thorough understanding of MSC biology. Despite the tremendous potential that exists for the clinical use of canine BM-MSCs in veterinary medicine, as well as in preclinical studies for human medicine, relatively little information exists regarding basic biological properties of the cells. In this study, we compared the importance of donor characteristics (age and harvest site) and ex vivo expansion on canine BM-MSC frequency (CFU-f) and differentiation potential...
2012: Cell Transplantation
Rose Eli Grassi Rici, Dayane Alcântara, Paula Fratini, Cristiane Valverde Wenceslau, Carlos Eduardo Ambrósio, Maria Angelica Miglino, Durvanei Augusto Maria
BACKGROUND: The bone morphogenetic proteins (BMPs) belong to a unique group of proteins that includes the growth factor TGF-β. BMPs play important roles in cell differentiation, cell proliferation, and inhibition of cell growth. They also participate in the maturation of several cell types, depending on the microenvironment and interactions with other regulatory factors. Depending on their concentration gradient, the BMPs can attract various types of cells and act as chemotactic, mitogenic, or differentiation agents...
2012: BMC Veterinary Research
M N Hall, W S Rosenkrantz, J H Hong, C E Griffin, C M Mendelsohn
Stem cells and their potential therapeutic uses in human and veterinary medicine have generated considerable interest. These cells have a number of potentially unique immunologic properties; most notable are their reported regenerative and antiinflammatory capabilities. The aim of this prospective pilot study was to evaluate the efficacy of intravenously administered autogenous adipose-derived mesenchymal stem cells (AD-MSCs) in the treatment of canine atopic dermatitis. AD-MSCs administered intravenously at a dose of 1...
2010: Veterinary Therapeutics: Research in Applied Veterinary Medicine
Linda L Black, James Gaynor, Dean Gahring, Cheryl Adams, Dennis Aron, Susan Harman, Daniel A Gingerich, Robert Harman
Autologous stem cell therapy in the field of regenerative veterinary medicine involves harvesting tissue, such as fat, from the patient, isolating the stem and regenerative cells, and administering the cells back to the patient. Autologous adipose-derived stem cell therapy has been commercially available since 2003, and the current study evaluated such therapy in dogs with chronic osteoarthritis of the hip. Dogs treated with adipose-derived stem cell therapy had significantly improved scores for lameness and the compiled scores for lameness, pain, and range of motion compared with control dogs...
2007: Veterinary Therapeutics: Research in Applied Veterinary Medicine
G Minkus, W Breuer, M Kirsch
Cases of diabetes mellitus in young cats and dogs (younger than five years) are described rarely in contrary to reports in older patients (older than eight years). Histological alterations of pancreatic islets in young canine and feline diabetic patients represent the main issue of this publication together with the regenerative capacity of insulin-producing cells in these patients. These regenerative phenomenons of differentiated islet cells (replication) or their progenitors (neogenesis deriving from stem cells) are detected in young cats and dogs in isolated cases whereas they are in general missing in older patients...
May 1998: Tierärztliche Praxis. Ausgabe K, Kleintiere/Heimtiere
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