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equipment contamination

Jennifer R DuBose, Zorana Matić, Maria Fernanda Wong Sala, Joel M Mumma, Colleen S Kraft, Lisa M Casanova, Kimberly Erukunuakpor, Francis T Durso, Victoria L Walsh, Puja Shah, Craig M Zimring, Jesse T Jacob
OBJECTIVE: To identify ways that the built environment may support or disrupt safe doffing of personal protective equipment (PPE) in biocontainment units (BCU). DESIGN: We observed interactions between healthcare workers (HCWs) and the built environment during 41 simulated PPE donning and doffing exercises. SETTING: The BCUs of 4 Ebola treatment facilities and 1 high-fidelity BCU mockup.ParticipantsA total of 64 HCWs (41 doffing HCWs and 15 trained observers) participated in this study...
June 18, 2018: Infection Control and Hospital Epidemiology
Selina Traxler, Ann-Christin Bischoff, Phillip Trefz, Jochen Schubert, Wolfram Miekisch
Volatile organic compound (VOC) profiles emitted in trace concentrations from bacteria or cells has gained increasing importance over the last decades. Analysis of VOCs in the headspace does not interfere with in vitro systems and, therefore, offers new options for noninvasive monitoring of cultures. Currently there is not any available standardized in-vitro sampling system which considers effects of dilution and contamination onto ppbV to pptV VOC concentrations during. In this study a new in-vitro system for online and offline headspace measurement of biological cultures was designed...
June 14, 2018: Journal of Breath Research
Sarah L Krein, Jeanmarie Mayer, Molly Harrod, Lauren E Weston, Lynn Gregory, Laura Petersen, Matthew H Samore, Frank A Drews
Importance: Using personal protective equipment (PPE) and transmission-based precautions are primary strategies for reducing the transmission of infectious agents. Objective: To identify and characterize failures in transmission-based precautions, including PPE use, by health care personnel that could result in self-contamination or transmission during routine, everyday hospital care. Design, Setting, and Participants: This qualitative study involved direct observation inside and outside patient rooms on clinical units from March 1, 2016, to November 30, 2016...
June 11, 2018: JAMA Internal Medicine
Luisa Garofalo, Alessia Mariacher, Rita Fanelli, Rosario Fico, Rita Lorenzini
In Western countries dogs and cats are the most popular pets, and people are increasingly opposed to their rearing for the fur industry. In 2007, a Regulation of the European Union (EU) banned the use and trade of dog and cat furs, but an official analytical protocol to identify them as source species was not provided, and violations of law are still frequent in all Member States. In this paper we report on the development and validation of a simple and affordable DNA method for species detection in furs to use as an effective tool to combat illegal trade in fur products...
2018: PeerJ
Corné Swart, Silvia Martínez-Jaime, Michal Gorka, Kerstin Zander, Alexander Graf
In-gel digestion has been used as a standard method for the preparation of protein samples for mass spectrometry analysis for over 25 years. Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The modified method, termed "High Throughput in Gel digestion" (HiT-Gel), is based on a 96-well plate format which results in a drastic reduction in labour intensity and sample handling...
June 5, 2018: Scientific Reports
Ahmadreza Yazdannik, Vajihe Atashi, Somayeh Ghafari
Background: Failure to provide proper respiratory care leads to incidence of certain complications such as ventilator-associated pneumonia. Nurses have a crucial role in providing this care. The aim of this study is to assess the performance of ICU (Intensive Care Unit) nurses in providing respiratory care. Materials and Methods: The present descriptive cross-sectional study recruited 120 nurses working in selected hospitals affiliated to Isfahan University of Medical Sciences from March to August 2016...
May 2018: Iranian Journal of Nursing and Midwifery Research
Jesse T Jacob, Loreen A Herwaldt, Francis T Durso
PURPOSE OF REVIEW: Human factors engineering (HFE) approaches are increasingly being used in healthcare, but have been applied in relatively limited ways to infection prevention and control (IPC). Previous studies have focused on using selected HFE tools, but newer literature supports a system-based HFE approach to IPC. RECENT FINDINGS: Cross-contamination and the existence of workarounds suggest that healthcare workers need better support to reduce and simplify steps in delivering care...
May 24, 2018: Current Opinion in Infectious Diseases
Gheorghe G Bălan, Irina Roşca, Elena-Laura Ursu, Florica Doroftei, Andra-Cristina Bostănaru, Eugen Hnatiuc, Valentin Năstasă, Vasile Şandru, Gabriela Ştefănescu, Anca Trifan, Mihai Mareş
Introduction: Duodenoscopes have been widely used for both diagnostic and therapeutic endoscopic retrograde cholangiopancreatography procedures. Numerous outbreaks of duodenoscope-associated infections involving multidrug-resistant bacteria have recently been reported. Plasma activated water (PAW) has been widely considered an effective agent for surface decontamination and is increasingly used for disinfection of medical equipment. The aim of this study was to evaluate whether the duodenoscopes currently on market are suited for the repeated use of PAW and to test the efficacy of PAW for their disinfection...
2018: Infection and Drug Resistance
Jerome Nriagu, Chuanwu Xi, Azhar Siddique, Annette Vincent, Basem Shomar
Deteriorating water quality from aging infrastructure, growing threat of pollution from industrialization and urbanization, and increasing awareness about waterborne diseases are among the factors driving the surge in worldwide use of point-of-entry (POE) and point-of-use (POU) filters. Any adverse influence of such consumer point-of-use systems on quality of water at the tap remains poorly understood, however. We determined the chemical and microbiological changes in municipal water from the point of entry into the household plumbing system until it leaves from the tap in houses equipped with filters...
May 29, 2018: Scientific Reports
Derick Nyabera Malavi, Tawanda Muzhingi, George Ooko Abong'
Limited information exists on the status of hygiene and probable sources of microbial contamination in Orange Fleshed Sweet Potato (OFSP) puree processing. The current study is aimed at determining the level of compliance to Good Manufacturing Practices (GMPs), hygiene, and microbial quality in OFSP puree processing plant in Kenya. Intensive observation and interviews using a structured GMPs checklist, environmental sampling, and microbial analysis by standard microbiological methods were used in data collection...
2018: International Journal of Food Science
Youngbeom Yu, Yang Hun Choi, Jaewon Choi, Soohoon Choi, Sung Kyu Maeng
The diversity of organic micropollutants (OMPs) in aquatic environments has been increasing rapidly during the last decade. Therefore, it is important to monitor and attenuate emerging contaminants before they can negatively affect the aquatic environment. However, due to the diversity and complexity of OMPs, there are limitations to using a single method for treating a combination of these pollutants. To address this issue, a mobile water treatment system (MWTS) equipped with different treatment units was designed to remove OMPs under field conditions...
May 20, 2018: Science of the Total Environment
Mahiyar F Nasarwanji, Jonisha Pollard, William Porter
Slips, trips, and falls from mobile mining equipment have been documented for decades. However, little research has been conducted to determine the events precipitating these incidents during ingress or egress. This study examined slips, trips, and falls sustained during ingress or egress from front-end loaders to determine the frequencies of factors that may contribute to injuries. Non-fatal injuries, when getting on or off of front-end wheel loaders specifically, were identified, coded, and analyzed from the Mine Safety and Health Administration's accidents, injuries, and illnesses database...
May 2018: International Journal of Industrial Ergonomics
Andrew Turner
Black products constitute about 15% of the domestic plastic waste stream, of which the majority is single-use packaging and trays for food. This material is not, however, readily recycled owing to the low sensitivity of black pigments to near infrared radiation used in conventional plastic sorting facilities. Accordingly, there is mounting evidence that the demand for black plastics in consumer products is partly met by sourcing material from the plastic housings of end-of-life waste electronic and electrical equipment (WEEE)...
May 17, 2018: Environment International
Hoda Sabati, Hossein Motamedi
OBJECTIVE: Water in oil emulsions increase oil processing costs and cause damage to refinery equipment which necessitates demulsification. Since chemical demulsifiers cause environmental pollution, biodemulsifiers have been paid more attention. This study aims to identify biodemulsifier-producing bacteria from petroleum contaminated environments. RESULTS: As a result, several biodemulsifier producing strains were found that Stenotrophomonas sp. strain HS7 (accession number: MF445088) which produced a cell associated biodemulsifier showed the highest demulsifying ratio, 98...
May 15, 2018: Biotechnology Letters
Moo-Been Chang, Yen-Chen Hsu, Shu-Hao Chang
A continuous pilot-scale system (CPS) equipped with effective air pollution control devices (APCDs) is used for remediating the sediments contaminated with PCDD/Fs, PCP and Hg simultaneously. The removal efficiencies of these three pollutants in sediments collected from seawater pond and river, respectively, are evaluated via thermal treatment processes. PAHs and CBz formed during thermal oxidation and pyrolysis are also analyzed for better understanding the behaviors of chlorinated organic compounds. Experimental results indicate that low-molecular-weight PAHs are closely related to the formation of CBz, PCDD/Fs, and CPs, while low chlorinated PCDD/Fs and CBz are predominant in flue gas with thermal oxidation...
May 7, 2018: Chemosphere
Joshua T Maxwell, Chin-Hsien Tsai, Tahmina A Mohiuddin, Jennifer Q Kwong
Ca2+ handling by mitochondria is a critical function regulating both physiological and pathophysiological processes in a broad spectrum of cells. The ability to accurately measure the influx and efflux of Ca2+ from mitochondria is important for determining the role of mitochondrial Ca2+ handling in these processes. In this report, we present two methods for the measurement of mitochondrial Ca2+ handling in both isolated mitochondria and cultured cells. We first detail a plate reader-based platform for measuring mitochondrial Ca2+ uptake using the Ca2+ sensitive dye calcium green-5N...
April 27, 2018: Journal of Visualized Experiments: JoVE
Andrea Paul, Lukas Wander, Roland Becker, Caroline Goedecke, Ulrike Braun
The increasing pollution of terrestrial and aquatic ecosystems with plastic debris leads to the accumulation of microscopic plastic particles of still unknown amount. To monitor the degree of contamination, analytical methods are urgently needed, which help to quantify microplastics (MP). Currently, time-costly purified materials enriched on filters are investigated both by micro-infrared spectroscopy and/or micro-Raman. Although yielding precise results, these techniques are time consuming, and are restricted to the analysis of a small part of the sample in the order of few micrograms...
May 12, 2018: Environmental Science and Pollution Research International
A M G A Laheij, J J de Soet, C M C Volgenant
The increasing resistance to antibiotics and the aging of the population of patients who visit the dental practice can lead to more frequent infections with (resistant) microorganisms. The prevention of cross contamination by the implementation of effective measures in the area of hygiene and procedures of infection control is therefore important. The furnishing of a dental practice can play an important part in prevention. What are the important issues in furnishing a practice? A distinction is made between 'critical' and 'non-critical' rooms...
May 2018: Nederlands Tijdschrift Voor Tandheelkunde
Shuang Mei, Yudan Wang, Guojun Wen, Yang Hu
Increasing deployment of optical fiber networks and the need for reliable high bandwidth make the task of inspecting optical fiber connector end faces a crucial process that must not be neglected. Traditional end face inspections are usually performed by manual visual methods, which are low in efficiency and poor in precision for long-term industrial applications. More seriously, the inspection results cannot be quantified for subsequent analysis. Aiming at the characteristics of typical defects in the inspection process for optical fiber end faces, we propose a novel method, “difference of min-max ranking filtering” (DO2MR), for detection of region-based defects, e...
May 3, 2018: Sensors
Dipendra Thapaliya, Jhalka Kadariya, Mike Capuano, Haleigh Rush, Clair Yee, Mark Oet, Sapana Lohani, Tara C Smith
BACKGROUND: Staphylococcus aureus is a major public health concern due to the emergence of virulent and drug resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA). Although numerous studies have been conducted to assess the environmental contamination of S. aureus in health-care and household settings, little is known about the prevalence and epidemiology of S. aureus, including MRSA, on environmental surfaces of children's playgrounds. This study investigated the prevalence and molecular epidemiology of S...
May 9, 2018: Pediatric Infectious Disease Journal
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