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equipment contamination

Linlin Cai, Huawei Wang, Lijiao Liang, Guangyu Wang, Xinglian Xu, Huhu Wang
Bacterial biofilms formed on equipment surfaces are potential sources of cross-contamination and can be responsible for the spread of bacteria involved in food spoilage, such as some Enterobacteriaceae family members. In this study, the effect of chlorite-based disinfectants, including sodium hypochlorite (SH), chlorine dioxide (CD), strongly acidic electrolyzed water (StAEW), and neutral electrolyzed water (NEW), on inactivation of mono-biofilms of Enterobacter cloacae, Klebsiella oxytoca, and Citrobacter freundii was evaluated separately...
April 18, 2018: Journal of Food Science
Jessica A Gray, P Scott Chandry, Mandeep Kaur, Chawalit Kocharunchitt, John P Bowman, Edward M Fox
High mortality and hospitalization rates have seen Listeria monocytogenes as a foodborne pathogen of public health importance for many years and of particular concern for high-risk population groups. Food manufactures face an ongoing challenge in preventing the entry of L. monocytogenes into food production environments (FPEs) due to its ubiquitous nature. In addition to this, the capacity of L. monocytogenes strains to colonize FPEs can lead to repeated identification of L. monocytogenes in FPE surveillance...
2018: Frontiers in Microbiology
M Martinasek, Z Rivera, A Ferrer, E Freundt
SETTING: Hookah smoking among young adults remains a public health threat. Increasing research has uncovered the deleterious effects of hookah smoking, including both acute and chronic health conditions. Due to the current lack of regulation, hookah bars/lounges lack protocols for equipment sanitation. OBJECTIVES: To examine evidence of bacterial contamination in hookah pipes due to a lack of sanitation regulations. DESIGN: For this field/laboratory study, 10 hookah bars/lounges were studied...
May 1, 2018: International Journal of Tuberculosis and Lung Disease
Chi-Ju Kim, Juhee Park, Vijaya Sunkara, Tae-Hyeong Kim, Yongjin Lee, Kyusang Lee, Mi-Hyun Kim, Yoon-Kyoung Cho
The potential utility of circulating tumour DNA (ctDNA) in patient blood for cancer diagnostics and real-time monitoring of disease progression is highly recognized. However, the lack of automated and efficient methods for cell-free DNA (cfDNA) isolation from peripheral blood has remained a challenge for broader acceptance of liquid biopsy in general clinical settings. Here, we demonstrate a lab-on-a-disc system equipped with newly developed, electromagnetically actuated, and reversible diaphragm valves that allows fully automated and rapid (<30 min) isolation of cfDNA from whole blood (>3 ml) to achieve high detection sensitivity by minimizing the degradation of fragile ctDNA as well as contamination of wild-type DNA from abundant blood cells...
April 16, 2018: Lab on a Chip
Elena Véliz, Teresa Vergara, Mercedes Pearcy, Jeannette Dabanch
Introduction Dental care has become a challenge for healthcare associated infection prevention programs, since the environment, within other factors, plays an important role in the transmission chain. Materials and Methods An intervention program was designed for the Dental Unit of Hospital Militar de Santiago, between years 2014 and 2015. The program contemplated 3 stages: diagnostic, intervention and evaluation stage. Objective To improve the safety of critical surfaces involved in dental healthcare. Results During the diagnostic stage, the cleaning and disinfection process was found to be deficient...
2018: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Sergio F Bodini, Marzio Malizia, Annalisa Tortelli, Luca Sanfilippo, Xingpeng Zhou, Roberta Arosio, Marzia Bernasconi, Stefano Di Lucia, Angela Manenti, Pompeo Moscetta
A novel tool, the DAMTA analyzer (Device for Analytical Monitoring and Toxicity Assessment), designed for fully automated toxicity measurements based on luminescent bacteria as well as for concomitant determination of chemical parameters, was developed and field-tested. The instrument is a robotic water analyzer equipped with a luminometer and a spectrophotometer, integrated on a thermostated reaction plate which contains a movable carousel with 80 cuvettes. Acute toxicity is measured on-line using a wild type Photobacterium phosphoreum strain with measurable bioluminescence and unaltered sensitivity to toxicants lasting up to ten days...
April 5, 2018: Ecotoxicology and Environmental Safety
David G Drewry, Lauren M Sauer, Kathryn Shaw-Saliba, Jennifer Therkorn, Kaitlin Rainwater-Lovett, Thomas Pilholski, Brian T Garibaldi
The Johns Hopkins Hospital created a biocontainment unit (BCU) to care for patients with highly infectious diseases while assuring healthcare worker safety. Research to date for BCU protocols and practices are based on case reports and lessons learned from patient care and exercises. This study seeks to be the first to explore the influences of healthcare worker movement and personal protective equipment (PPE) doffing on the transport of simulant pathogen particles in a BCU. A cough device released 1 μm fluorescent polystyrene beads (PSLs) in the patient room...
March 2018: Health Security
Omar Laraqui, Nadia Manar, Salwa Laraqui, Tarik Ghailan, Frédéric Deschamps, Reda Hammouda, Chakib El Houssine Laraqui
BACKGROUND: The main objective was to evaluate the prevalence of skin diseases amongst fishermen, to specify the different clinical forms, associations and most frequent localisations, and to identify potential aetiological factors. The secondary objectives were to assess the knowledge, attitudes and practices of seafarers regarding occupational dermatoses to conduct information, awareness and education campaigns on risk factors and behaviours and to propose adapted prevention. MATERIALS AND METHODS: This cross-sectional survey involved 1102 artisanal fishermen who attended the annual legal medical consultation at the occupational health service...
2018: International Maritime Health
Andie S Lee, Elizabeth White, Leigh G Monahan, Slade O Jensen, Raymond Chan, Sebastiaan J van Hal
OBJECTIVETo describe the transmission dynamics of the emergence and persistence of vanA vancomycin-resistant enterococcus (VRE) in an intensive care unit (ICU) using whole-genome sequencing of patient and environmental isolates.DESIGNRetrospective cohort study.SETTINGICU in a tertiary referral center.PARTICIPANTSPatients admitted to the ICU over an 11-month period.METHODS VanA VRE isolated from patients (n=31) were sequenced using the Illumina MiSeq platform. Environmental samples from bed spaces, equipment, and waste rooms were collected...
April 3, 2018: Infection Control and Hospital Epidemiology
Valentina Innocenzi, Francesco Tortora, Marina Prisciandaro, Ida De Michelis, Francesco Vegliò, Giuseppe Mazziotti di Celso
Hydrometallurgical processes for the treatment and recovery of metals from waste electrical and electronic equipment produce wastewaters containing heavy metals. These residual solutions cannot be discharged into the sewer without an appropriate treatment. Specific wastewater treatments integrated with the hydrometallurgical processes ensure a sustainable recycling loops of the electrical wastes to maximize the metals recovery and minimize the amount of wastes and wastewaters produced. In this research activity the efficiency of ultrafiltration combined with surfactant micelles (micellar-enhanced ultrafiltration) was tested to remove metals form leach liquors obtained after leaching of NiMH spent batteries...
March 27, 2018: Journal of Environmental Management
Bruce W Chaffee, Michael J Lander, Catherine Christen, Kimberly A Redic
Purpose The primary aim was to determine if dispensing of cyclophosphamide tablets resulted in accumulated residue on pharmacy counting tools during a simulated outpatient dispensing process. Secondary objectives included determining if cyclophosphamide contamination exceeded a defined threshold level of 1 ng/cm2 and if a larger number of prescriptions dispensed resulted in increased contamination. Methods Mock prescriptions of 40 cyclophosphamide 50 mg tablets were counted on clean trays in three scenarios using a simulated outpatient pharmacy after assaying five cleaned trays as controls...
January 1, 2018: Journal of Oncology Pharmacy Practice
Amber Arnold, Graham Bickler, Thomas Stephen Harrison
Background: Part 2A Orders are the legal means which allow local authorities (LAs), upon application to court, to exercise powers over persons, things or premises to protect public health. Methods: We surveyed lead professionals involved in applications to understand the use and utility of such Orders since their inception in April 2010 to July 2015. Results: All applications for Orders were granted; 29 for persons (28 for tuberculosis, 1 for HIV); these were renewed in 18 (18/25, 72%) cases up to seven times; 23 applications related to things (tattoo and piercing equipment); and three applications related to 'premises' (Escherichia coli 0137 on farm, faecal contamination)...
March 24, 2018: Journal of Public Health
Jing Meng, Tieyu Wang, Shuai Song, Pei Wang, Qifeng Li, Yunqiao Zhou, Yonglong Lu
With the shift of fluorine chemical industry from developed countries to China and increasing demand for fluorine chemical products, occurrence of perfluoroalkyl substances (PFASs) in production and application areas has attracted more attention. In this study, 153 soil samples were collected from 21 cities along the urbanizing coastal area of the Bohai and Yellow Seas. PFASs in this area were relatively higher, compared with other study areas. The concentrations ranged from 2.76 to 64.0 ng g-1 , and those in most sites were between 2...
March 24, 2018: Environmental Pollution
Anna Ławniczek-Wałczyk, Marcin Cyprowski, Małgorzata Gołofit-Szymczak, Angelina Wójcik-Fatla, Violetta Zając, Rafał L Górny
BACKGROUND: <sup></sup>In recent years, the number of people suffering from diseases caused by fungi has been increasing. However, knowledge of the biodiversity of fungal pathogens in the work environment is still insufficient. The aim of this work was to evaluate the exposure to fungi being disseminated in the air of workplaces contaminated with organic dust of plant and animal origin. MATERIAL AND METHODS: Bioaerosol samples were collected at 3 occupational settings (poultry farm, biomass burning power plant and wastewater treatment plant) using button samplers...
March 21, 2018: Medycyna Pracy
Melanie L Yarbrough, Jennie H Kwon, Meghan A Wallace, Tiffany Hink, Angela Shupe, Victoria J Fraser, Erik R Dubberke, Carey-Ann D Burnham
Laboratory testing to support the care of patients with highly infectious diseases may pose a risk for laboratory workers. However, data on the risk of virus transmission during routine laboratory testing conducted using standard personal protective equipment (PPE) are sparse. Our objective was to measure laboratory contamination during routine analysis of patient specimens. Remnant specimens were spiked with the nonpathogenic MS2 bacteriophage at 1.0 x 107 PFU/mL and biomarker contamination was assessed using RT-PCR for MS2...
March 21, 2018: Journal of Clinical Microbiology
E Gonzalez, F E Pitre, A P Pagé, J Marleau, W Guidi Nissim, M St-Arnaud, M Labrecque, S Joly, E Yergeau, N J B Brereton
BACKGROUND: One method for rejuvenating land polluted with anthropogenic contaminants is through phytoremediation, the reclamation of land through the cultivation of specific crops. The capacity for phytoremediation crops, such as Salix spp., to tolerate and even flourish in contaminated soils relies on a highly complex and predominantly cryptic interacting community of microbial life. METHODS: Here, Illumina HiSeq 2500 sequencing and de novo transcriptome assembly were used to observe gene expression in washed Salix purpurea cv...
March 21, 2018: Microbiome
A Leiva, F Granados-Chinchilla, M Redondo-Solano, M Arrieta-González, E Pineda-Salazar, A Molina
Animal by-product rendering establishments are still relevant industries worldwide. Animal by-product meal safety is paramount to protect feed, animals, and the rest of the food chain from unwanted contamination. As microbiological contamination may arise from inadequate processing of slaughterhouse waste and deficiencies in good manufacturing practices within the rendering facilities, we conducted an overall establishment's inspection, including the product in several parts of the process.An evaluation of the Good Manufacturing Practices (GMP) was carried out, which included the location and access (i...
March 19, 2018: Poultry Science
Joseph Wambui, Peter Lamuka, Edward Karuri, Joseph Matofari, Patrick Murigu Kamau Njage
The microbial contamination level profiles (MCLPs) attributed to contamination of beef carcasses, personnel, and equipment in five Kenyan small and medium enterprise slaughterhouses were determined. Aerobic plate counts, Enterobacteriaceae, Staphylococcus, and Salmonella were used to determine contamination at four different slaughter stages, namely, dehiding, evisceration, splitting, and dispatch. Microbiological criteria of the four microorganisms were used to score contamination levels (CLs) as poor (0), poor to average (1), average (2), or good (3)...
April 2018: Journal of Food Protection
Shakthi Rk Devan, Suresh Vasu, Yogesha Mallikarjuna, Ramkumar Ponraj, Gireesh Kamath, Suresh Poosala
Biodecontamination is important for eliminating pathogens at research animal facilities, thereby preventing contamination within barrier systems. We enhanced our facility's standard biodecontamination method to replace the traditional foggers, and the new system was used effectively after creating bypass ducts in HVAC units so that individual rooms could be isolated. The entire system was controlled by inhouse-developed supervisory control and data-acquisition software that supported multiple cycles of decontamination by equipment, which had different decontamination capacities, operated in parallel, and used different agents, including H2O2 vapor and ClO2 gas...
March 1, 2018: Journal of the American Association for Laboratory Animal Science: JAALAS
Syahir Habib, Siti Aqlima Ahmad, Wan Lutfi Wan Johari, Mohd Yunus Abd Shukor, Siti Aisyah Alias, Khalilah Abdul Khalil, Nur Adeela Yasid
BACKGROUND: Biodegradation of hydrocarbons in Antarctic soil has been reported to be achieved through the utilisation of indigenous cold-adapted microorganisms. Although numerous bacteria isolated from hydrocarbon-contaminated sites in Antarctica were able to demonstrate promising outcomes in utilising hydrocarbon components as their energy source, reports on the utilisation of hydrocarbons by strains isolated from pristine Antarctic soil are scarce. In the present work, two psychrotolerant strains isolated from Antarctic pristine soil with the competency to utilise diesel fuel as the sole carbon source were identified and optimised through conventional and response surface method...
March 17, 2018: Microbial Cell Factories
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