Xiao Wang, Xiaobao Deng, Yidun Zhang, Weiyi Dong, Qiao Rao, Qingmei Huang, Fei Tang, Rong Shen, Hongzhi Xu, Zhen Jin, Youzhi Tang, Dan Du
The recent global upsurge in Monkeypox virus (MPXV) outbreaks underscores the critical need for rapid and precise diagnostic solutions, particularly in resource-constrained settings. The gold standard diagnostic method, qRT-PCR, is hindered by its time-consuming nature, requirement for nucleic acid purification, expensive equipment, and the need for highly trained personnel. Traditional CRISPR/Cas fluorescence assays, relying on trans-cleavage of ssDNA/RNA reporters labeled with costly fluorophores and quenchers, pose challenges that limit their widespread application, especially for point-of-care testing (POCT)...
April 16, 2024: Biosensors & Bioelectronics