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https://www.readbyqxmd.com/read/28931453/molecular-characterization-and-distribution-of-plasmodium-matutinum-a-common-avian-malaria-parasite
#1
Gediminas Valkiūnas, Mikas Ilgūnas, Dovilė Bukauskaitė, Vaidas Palinauskas, Rasa Bernotienė, Tatjana A Iezhova
Species of Plasmodium (Plasmodiidae, Haemosporida) are widespread and cause malaria, which can be severe in avian hosts. Molecular markers are essential to detect and identify parasites, but still absent for many avian malaria and related haemosporidian species. Here, we provide first molecular characterization of Plasmodium matutinum, a common agent of avian malaria. This parasite was isolated from a naturally infected thrush nightingale Luscinia luscinia (Muscicapidae). Fragments of mitochondrial, apicoplast and nuclear genomes were obtained...
September 21, 2017: Parasitology
https://www.readbyqxmd.com/read/28918832/streptococcus-anginosus-group-bacterial-infections
#2
Tasaduq Fazili, Scott Riddell, Deanna Kiska, Tim Endy, Luca Giurgea, Calden Sharngoe, Waleed Javaid
BACKGROUND: The Streptococcus anginosus group (SAG) causes a variety of infections in adults. To better understand the burden of SAG infections and their associated morbidity and mortality, we conducted a retrospective analysis of these infections in adults at a tertiary care center. METHODS: A retrospective review of all cultures positive for SAG in adults and a corresponding review of the patients' medical records were conducted at a tertiary care facility in central New York...
September 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/28915435/an-overview-of-systematic-reviews-on-the-public-health-consequences-of-social-isolation-and-loneliness
#3
REVIEW
N Leigh-Hunt, D Bagguley, K Bash, V Turner, S Turnbull, N Valtorta, W Caan
OBJECTIVES: Social isolation and loneliness have been associated with ill health and are common in the developed world. A clear understanding of their implications for morbidity and mortality is needed to gauge the extent of the associated public health challenge and the potential benefit of intervention. STUDY DESIGN: A systematic review of systematic reviews (systematic overview) was undertaken to determine the wider consequences of social isolation and loneliness, identify any differences between the two, determine differences from findings of non-systematic reviews and to clarify the direction of causality...
September 12, 2017: Public Health
https://www.readbyqxmd.com/read/28902609/knee-extension-loss-secondary-to-a-cyclops-like-gouty-tophus-a-case-report-and-literature-review
#4
Michael Doany, Nicole Lopez, Andrew Rokito
While gouty arthritis of the knee is not uncommon, associated mechanical block to extension is a rarely seen complication. This report presents a unique case of extension loss due to a single, isolated intra-articular gouty tophus. Only a few similar reports have been described in the literature involving cases that are often initially suspected to be related to inherent structural knee pathology as opposed to a systemic condition or illness.
May 2017: Bulletin of the Hospital for Joint Diseases
https://www.readbyqxmd.com/read/28890518/a-case-of-imported-dengue-fever-from-burkina-faso-to-japan-in-october-2016
#5
Takehiro Hashimoto, Satoshi Kutsuna, Takahiro Maeki, Shigeru Tajima, Saho Takaya, Yuichi Katanami, Kei Yamamoto, Nozomi Takeshita, Kayoko Hayakawa, Yasuyuki Kato, Shuzo Kanagawa, Norio Ohmagari
Dengue fever remains underreported in Africa due to lack of awareness among healthcare providers, the presence of other febrile illnesses, and insufficient laboratory testing. We present a case of imported dengue fever from Burkina Faso to Japan, where an outbreak of dengue was reported on October 18, 2016. The phylogenetic analysis revealed that the isolate from our patient belonged to a distinct of sylvatic dengue viruses, suggesting that dengue viruses have been maintained in the mosquitoes and human cycles in Burkina Faso for more than 30 years...
September 11, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28884123/deviation-of-the-fecal-stream-in-colonic-bowel-segments-results-in-increased-numbers-of-isolated-lymphoid-follicles-in-the-submucosal-compartment-in-a-novel-murine-model-of-diversion-colitis
#6
Annabel Kleinwort, Paula Döring, Christine Hackbarth, Claus-Dieter Heidecke, Tobias Schulze
INTRODUCTION: Diversion colitis is a significant health problem due to its high incidence in patients with diverting enterostomy. This mucosal inflammation presents characteristic histopathological features allowing for the differentiation of this entity from other inflammatory bowel diseases. The pathophysiology of this disease remains ill-defined, in part due to the lack of appropriate animal models. The present study was performed in order to develop and characterize a murine model of diversion colitis...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28883819/immunogenicity-of-dna-vaccine-against-h5n1-containing-extended-kappa-b-site-in-vivo-study-in-mice-and-chickens
#7
Patrycja Redkiewicz, Anna Stachyra, Róz A Sawicka, Katarzyna Bocian, Anna Góra-Sochacka, Piotr Kosson, Agnieszka Sirko
Influenza is one of the most important illnesses in the modern world, causing great public health losses each year due to the lack of medication and broadly protective, long-lasting vaccines. The development of highly immunogenic and safe vaccines is currently one of the major problems encountered in efficient influenza prevention. DNA vaccines represent a novel and powerful alternative to the conventional vaccine approaches. To improve the efficacy of the DNA vaccine against influenza H5N1, we inserted three repeated kappa B (κB) motifs, separated by a 5-bp nucleotide spacer, upstream of the cytomegalovirus promoter and downstream of the SV40 late polyadenylation signal...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28878022/drusen-in-patient-derived-hipsc-rpe-models-of-macular-dystrophies
#8
Chad A Galloway, Sonal Dalvi, Sandy S C Hung, Leslie A MacDonald, Lisa R Latchney, Raymond C B Wong, Robyn H Guymer, David A Mackey, David S Williams, Mina M Chung, David M Gamm, Alice Pébay, Alex W Hewitt, Ruchira Singh
Age-related macular degeneration (AMD) and related macular dystrophies (MDs) are a major cause of vision loss. However, the mechanisms underlying their progression remain ill-defined. This is partly due to the lack of disease models recapitulating the human pathology. Furthermore, in vivo studies have yielded limited understanding of the role of specific cell types in the eye vs. systemic influences (e.g., serum) on the disease pathology. Here, we use human induced pluripotent stem cell-retinal pigment epithelium (hiPSC-RPE) derived from patients with three dominant MDs, Sorsby's fundus dystrophy (SFD), Doyne honeycomb retinal dystrophy/malattia Leventinese (DHRD), and autosomal dominant radial drusen (ADRD), and demonstrate that dysfunction of RPE cells alone is sufficient for the initiation of sub-RPE lipoproteinaceous deposit (drusen) formation and extracellular matrix (ECM) alteration in these diseases...
September 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28867170/assessment-of-pit-latrines-in-a-peri-urban-community-in-kwazulu-natal-south-africa-as-a-source-of-antibiotic-resistant-e-coli-strains
#9
Lorika S Beukes, Tracy L B King, Stefan Schmidt
Due to the frequent use of antibiotics and recurring illnesses related to multidrug-resistant (MDR) bacteria in South Africa, we determined if MDR Escherichia coli were present in pit latrine fecal sludge samples obtained from a peri-urban community in KwaZulu-Natal, South Africa. The abundance of E. coli in pit latrine samples was established using a most probable number (MPN) method with species confirmation done using biochemical tests and polymerase chain reaction (PCR). Forty-four randomly selected E. coli pit latrine isolates were further characterized, using the European committee on antimicrobial susceptibility testing (EUCAST) disk diffusion method to establish antibiotic resistance profiles for these E...
August 12, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28835578/altered-immune-responses-in-broiler-chicken-husbandry-workers-and-their-association-with-endotoxin-exposure
#10
Ravi Gautam, Yong Heo, GyeongDong Lim, EunSeob Song, Katharine Roque, JaeHee Lee, YeonGyeong Kim, AhRang Cho, SoJung Shin, ChangYul Kim, GiHwan Bang, JiYun Bahng, HyoungAh Kim
Exposure to bioaerosols in indoor animal farms associates with respiratory illnesses, but little is known about the immune modulation to chicken farmers. This study aimed to compare the general immunity of chicken farmers with those of control subjects with non-agricultural jobs. Blood taken from the farmers and controls was subjected to plasma IgE and IgG subclass measurements. Isolated peripheral blood mononuclear cells (PBMC) were stimulated and cytokine production was measured. Indoor total and respirable dust levels and their endotoxin (LPS) and aflatoxin (AF) levels in the farms were measured...
August 22, 2017: Industrial Health
https://www.readbyqxmd.com/read/28826328/refugee-and-immigrant-women-s-experience-of-postpartum-depression-a-meta-synthesis
#11
Carolyn L Tobin, Pam Di Napoli, Cheryl Tatano Beck
The aim of this work is to synthesize qualitative research on refugee and immigrant women's experiences of postpartum depression (PPD) to gain insight into the unique needs of this group of women. This population is more at risk of developing PPD due to a complexity of issues including pre- and postmigratory stressors; however, there is currently little research on this topic available to health care providers and policy makers. Thirteen articles met inclusion criteria, and five themes emerged from the meta-synthesis: (a) suffering in solitude, (b) the invisible illness, (c) cultural conceptualizations, (d) barriers to help seeking, and (e) facilitators of help seeking...
January 1, 2017: Journal of Transcultural Nursing: Official Journal of the Transcultural Nursing Society
https://www.readbyqxmd.com/read/28804559/microrna-1907-enhances-atherosclerosis-associated-endothelial-cell-apoptosis-by-suppressing-bcl-2
#12
Jin Zhao, Shu-Lin Ou, Wei-You Wang, Chang Yan, Lu-Xiang Chi
Injury and endothelial cell apoptosis are hall marks of atherosclerosis (AS). However, the mechanisms underlying its pathogenesis remain ill-defined. Recent evidence of a role for microRNAs in AS-associated endothelial cell apoptosis encouraged us to address this question. Here, AS was developed in ApoE (-/-) mice supplied with a high-fat diet (HFD), compared to ApoE (-/-) mice supplied with a normal diet (ND). Mouse endothelial cells were isolated from the aortic arch using flow cytometry based on their expression of CD31...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28799681/genomic-and-clinical-evidence-uncovers-the-enterohepatic-species-helicobacter-valdiviensis-as-a-potential-human-intestinal-pathogen
#13
Pablo Fresia, Ronald Jara, Rafael Sierra, Ignacio Ferrés, Gonzalo Greif, Gregorio Iraola, Luis Collado
BACKGROUND: Helicobacter valdiviensis is a recently described enterohepatic species isolated from wild bird's fecal samples. Currently, its pathogenic potential and clinical significance are unknown mainly due to the lack of whole-genome sequences to compare with other helicobacters and the absence of specific screenings to determine its prevalence in humans. MATERIALS AND METHODS: The species type strain (WBE14(T) ) was whole-genome-sequenced, and comparative analyses were carried out including the genomes from other Helicobacter species to determine the exact phylogenetic position of H...
August 11, 2017: Helicobacter
https://www.readbyqxmd.com/read/28797733/propagation-of-respiratory-viruses-in-human-airway-epithelia-reveals-persistent-virus-specific-signatures
#14
Manel Essaidi-Laziosi, Francisco Brito, Sacha Benaoudia, Léna Royston, Valeria Cagno, Mélanie Fernandes-Rocha, Isabelle Piuz, Evgeny Zdobnov, Song Huang, Samuel Constant, Marc-Olivier Boldi, Laurent Kaiser, Caroline Tapparel
BACKGROUND: Leading etiologies of acute illnesses, respiratory viruses typically cause self-limited diseases, though severe complications can occur in fragile patients. Rhinoviruses, respiratory enteroviruses, influenza virus, respiratory syncytial viruses and coronaviruses are highly prevalent respiratory pathogens, but due to the lack of reliable animal models, their differential pathogenesis remains poorly characterized. OBJECTIVE: To compare infections by respiratory viruses isolated from clinical specimens using reconstituted human airway epithelia...
August 7, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28777716/research-priorities-in-subspecialty-palliative-care-policy-initiatives
#15
Lynn F Reinke, Diane E Meier
Palliative care demonstrably improves quality of life for the seriously ill in a manner that averts preventable health crises and their associated costs. Because of these outcomes, palliative care is now broadening its reach beyond hospitals, and hospice care for those near death, to patients and their families living in the community with chronic multimorbidities that have uncertain or long expected survival. In this article, we address research needed to enable policies supportive of palliative care access and quality, including changes in regulatory, accreditation, financing, and training approaches in the purview of policy makers...
August 2017: Journal of Palliative Medicine
https://www.readbyqxmd.com/read/28757139/clinical-and-epidemiological-characteristics-of-human-parainfluenza-virus-infections-of-children-in-southern-taiwan
#16
Kai-Wen Wu, Shih-Min Wang, Ching-Fen Shen, Tzong-Shiann Ho, Jen-Ren Wang, Ching-Chuan Liu
BACKGROUND: Human parainfluenza viruses (HPIV) 1-4 had been analyzed as being one of the most frequent causes of hospitalizations for young children with respiratory tract illnesses. METHODS: This retrospective study was performed from children virologically confirmed as HPIV infection through throat swab or nasopharyngeal aspirates at a tertiary care university hospital, between January 2012 and December 2014. HPIV4 was not checked and analyzed, due to not include in the commercial kit...
June 22, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28754148/nosocomial-bacterial-infections-and-their-antimicrobial-susceptibility-patterns-among-patients-in-ugandan-intensive-care-units-a-cross-sectional-study
#17
Peter Agaba, Janat Tumukunde, J V B Tindimwebwa, Arthur Kwizera
BACKGROUND: The intensive care unit (ICU) admits critically ill patients requiring advanced airway, respiratory, cardiac and renal support. Despite the highly-specialized interventions, the mortality and morbidity is still high due to a number of reasons including nosocomial infections, which are the most likely complications in hospitalized patients with the rates being highest among ICU patients. METHODS: In this cross-sectional study of 111 adult patients admitted to 2 of the ICUs in Uganda, we set out to describe the commonest bacterial infections, their antimicrobial susceptibility patterns and factors associated with development of a nosocomial infection...
July 28, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28723693/upregulation-of-microrna-876-induces-endothelial-cell-apoptosis-by-suppressing-bcl-xl-in-development-of-atherosclerosis
#18
Kaicheng Xu, Peng Liu, Yue Zhao
BACKGROUND/AIMS: The injury and apoptotic cell death of endothelial cells hallmark the development of atherosclerosis (AS), characterized by dysregulation of lipid homeostasis, immune responses, and formation of coronary plaques. However, the mechanisms underlying the initiation of endothelial cell apoptosis remain ill-defined. Recent evidence suggests a role of microRNAs in the processes of AS-associated endothelial cell apoptosis. Thus, we studied this question in the current study...
July 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28720009/the-effect-of-isolated-finger-stiffness-on-adjacent-digit-function
#19
Rasha Baaqeel, Kitty Wu, Shrikant J Chinchalkar, Douglas C Ross
BACKGROUND: Isolated stiffness in a single finger can affect the function of adjacent digits and decrease overall hand function due to the quadriga phenomenon. This study objectively quantifies the dysfunctional impact of each individual stiff finger upon the remaining digits. METHODS: Twenty-five individuals (10 men and 15 women) with a mean age of 31 years (range, 18-58 years) without any upper limb pathology, neuropathy, or systemic illness were recruited. Volar-based finger splints were used to hold individual digits of the dominant hand (24 right and 1 left) sequentially in full extension at the metacarpophalangeal (MCP), proximal interphalangeal (PIP), and distal interphalangeal (DIP) joints...
March 1, 2017: Hand: Official Journal of the American Association for Hand Surgery
https://www.readbyqxmd.com/read/28718274/-microbiome-and-next-generation-sequencing
#20
REVIEW
A Suárez Moya
The human microbiome is an internal ecosystem that refers to the community of microorganisms that populate the human body. These microorganisms are essential to support his health, because the interaction between the host immune system and microorganisms, provide the host with protection against pathogens, and contributes to the preservation of health. Bacteriological culture has been the basis for traditional microbiology; however, most of the bacterial forms observed in nature cannot be isolated with laboratory culture methods...
July 17, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
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