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https://www.readbyqxmd.com/read/29338017/comparative-genomics-identifies-distinct-lineages-of-s-enteritidis-from-queensland-australia
#1
Rikki M A Graham, Lester Hiley, Irani U Rathnayake, Amy V Jennison
Salmonella enterica is a major cause of gastroenteritis and foodborne illness in Australia where notification rates in the state of Queensland are the highest in the country. S. Enteritidis is among the five most common serotypes reported in Queensland and it is a priority for epidemiological surveillance due to concerns regarding its emergence in Australia. Using whole genome sequencing, we have analysed the genomic epidemiology of 217 S. Enteritidis isolates from Queensland, and observed that they fall into three distinct clades, which we have differentiated as Clades A, B and C...
2018: PloS One
https://www.readbyqxmd.com/read/29319754/identification-and-antimicrobial-suceptibility-profile-of-bacteria-causing-bovine-mastitis-from-dairy-farms-in-pelotas-rio-grande-do-sul
#2
C H Freitas, J F Mendes, P V Villarreal, P R Santos, C L Gonçalves, H L Gonzales, P S Nascente
Mastitis is an inflammatory process of the udder tissue caused mainly by the bacteria Staphylococcus aureus. The indiscriminate use of antibiotics fosters conditions that favor the selection of resistant microorganisms, suppressing at the same time susceptible forms, causing a serious problem in dairy cattle. Given the importance in performing an antibiogram to select the most adequate antimicrobial therapy, the aim of this study was to identify bacteria isolated from cow's milk with mastitis, in dairy farms situated in the city of Pelotas, Rio Grande do Sul, and to determinate the susceptibility profile of these isolates against the antibiotics used to treat this illness...
January 8, 2018: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/29300761/investigation-of-prevalence-of-free-shiga-toxin-producing-escherichia-coli-stec-specific-bacteriophages-and-its-correlation-with-stec-bacterial-hosts-in-a-produce-growing-area-in-salinas-california
#3
Yen-Te Liao, Irwin A Quintela, Kimberly Nguyen, Alexandra Salvador, Michael B Cooley, Vivian C H Wu
Shiga toxin-producing E. coli (STEC) causes approximately 265,000 illnesses and 3,600 hospitalizations annually and is highly associated with animal contamination due to the natural reservoir of ruminant gastrointestinal tracts. Free STEC-specific bacteriophages against STEC strains are also commonly isolated from fecal-contaminated environment. Previous studies have evaluated the correlation between the prevalence of STEC-specific bacteriophages and STEC strains to improve animal-associated environment. However, the similar information regarding free STEC-specific bacteriophages prevalence in produce growing area is lacking...
2018: PloS One
https://www.readbyqxmd.com/read/29291191/experiences-of-unemployment-and-well-being-after-job-loss-during-economic-recession-results-of-a-qualitative-study-in-east-central-sweden
#4
Anne-Sofie Hiswåls, Anneli Marttila, Emelie Mälstam, Gloria Macassa
Introduction: Several studies have revealed an association between unemployment and ill health, and shown that unemployment can affect people differently. This study aimed to provide an understanding of the experiences of unemployment and perceptions of wellbeing among persons who involuntary lost their work during the recent economic recession in Gävle Municipality. Methods: Sixteen unemployed men and women aged 28-62 were interviewed face-to-face. A purposeful sampling strategy was used in order to suit the research question and to increase the variation among informants...
December 13, 2017: Journal of Public Health Research
https://www.readbyqxmd.com/read/29284913/telemental-health-care-an-effective-alternative-to-conventional-mental-care-a-systematic-review
#5
Mostafa Langarizadeh, Mohsen S Tabatabaei, Kamran Tavakol, Majid Naghipour, Alireza Rostami, Fatemeh Moghbeli
Background and Objectives: Due to the high costs of conventional mental health care, there has been a rise in the application of web-based technologies in recent years, i.e., telemental health care. We conducted this systematic review in 2017, using high quality research articles on the applications, technologies, advantages and challenges associated with telemental health care published since year 2000. Methods: We used a combination of relevant key words to search four major databases, such as "Web of Sciences, Embase, PubMed and Science Direct"...
December 2017: Acta Informatica Medica: AIM
https://www.readbyqxmd.com/read/29284248/streptococcus-suis-a-re-emerging-pathogen-associated-with-occupational-exposure-to-pigs-or-pork-products-part-i-epidemiology
#6
Jacek Dutkiewicz, Jacek Sroka, Violetta Zając, Bernard Wasiński, Ewa Cisak, Anna Sawczyn, Anna Kloc, Angelina Wójcik-Fatla
<i>Streptococcus suis</i> (ex Elliot 1966, Kilpper-Bälz & Schleifer 1987) is a facultatively anaerobic Gram-positive ovoid or coccal bacterium surrounded by a polysaccharide capsule. Based on the antigenic diversity of the capsule, <i>S. suis</i> strains are classified serologically into 35 serotypes. <i>Streptococcus suis</i> is a commensal of pigs, commonly colonizing their tonsils and nasal cavities, mostly in weaning piglets between 4-10 weeks of age. This species occurs also in cattle and other mammals, in birds and in humans...
December 23, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/29276241/development-of-institutional-guidelines-for-management-of-gram-negative-bloodstream-infections-incorporating-local-evidence
#7
Elizabeth B Nimmich, P Brandon Bookstaver, Joseph Kohn, Julie Ann Justo, Katie L Hammer, Helmut Albrecht, Majdi N Al-Hasan
Background: Appropriate empirical antimicrobial therapy is associated with improved outcomes of patients with Gram-negative bloodstream infections (BSI). Objective: Development of evidence-based institutional management guidelines for empirical antimicrobial therapy of Gram-negative BSI. Methods: Hospitalized adults with Gram-negative BSI in 2011-2012 at Palmetto Health hospitals in Columbia, SC, USA, were identified. Logistic regression was used to examine the association between site of infection acquisition and BSI due to Pseudomonas aeruginosa or chromosomally mediated AmpC-producing Enterobacteriaceae (CAE)...
November 2017: Hospital Pharmacy
https://www.readbyqxmd.com/read/29261162/food-borne-outbreak-investigation-and-molecular-typing-high-diversity-of-staphylococcus-aureus-strains-and-importance-of-toxin-detection
#8
Sarah Denayer, Laurence Delbrassinne, Yacine Nia, Nadine Botteldoorn
Staphylococcus aureus is an important aetiological agent of food intoxications in the European Union as it can cause gastro-enteritis through the production of various staphylococcal enterotoxins (SEs) in foods. Reported enterotoxin dose levels causing food-borne illness are scarce and varying. Three food poisoning outbreaks due to enterotoxin-producing S. aureus strains which occurred in 2013 in Belgium are described. The outbreaks occurred in an elderly home, at a barbecue event and in a kindergarten and involved 28, 18, and six cases, respectively...
December 20, 2017: Toxins
https://www.readbyqxmd.com/read/29220754/association-of-high-mortality-with-extended-spectrum-%C3%AE-lactamase-esbl-positive-cultures-in-community-acquired-infections
#9
Sumit Ray, Dimple Anand, Sankalp Purwar, Arijit Samanta, Kaustubh V Upadhye, Prasoon Gupta, Debashis Dhar
PURPOSE: Infections due to multidrug resistant organisms have become a serious health concern worldwide. The present study was conducted to investigate the spectrum of microbial resistance pattern in the community and their effects on mortality. METHODS: A retrospective review and analysis of prospectively collected data was done of all patients admitted with diagnosis of sepsis in two tertiary care ICU's for a period of two years. Demographics, culture positivity, microbial spectrum, resistance pattern and outcome data were collected...
October 31, 2017: Journal of Critical Care
https://www.readbyqxmd.com/read/29217126/fast-protocol-for-the-production-of-histoplasma-capsulatum-antigens-for-antibody-detection-in-the-immunodiagnosis-of-histoplasmosis
#10
Roseli Santos de Freitas, Camila Mika Kamikawa, Adriana Pardini Vicentini
BACKGROUND: Current methods for the production of Histoplasma capsulatum antigens are problematic in terms of standardization, specificity, stability, repeatability and reproducibility. AIMS: In this study, we sought to optimize the methodology for producing H. capsulatum antigens, and to evaluate its applicability. METHODS: Antigenic preparations obtained from 12 H. capsulatum isolates were evaluated by double immunodiffusion and immunoblotting assays against homologous and heterologous sera...
December 4, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/29207703/diagnosis-of-acute-q-fever-by-detection-of-coxiella-burnetii-dna-using-real-time-pcr-employing-a-commercial-genesig-easy-kit
#11
Jothimani Pradeep, Selvaraj Stephen, Stanley Ambroise, Dhandapany Gunasekaran
Introduction: Query (Q) fever is an important zoonosis and a cause of concern for humans, due to the potential bioterrorism threat posed by the causative agent, Coxiella burnetii. Because of the danger of contracting the illness, isolation attempts are seldom made. Serological and molecular diagnostic tests are the main option. Aim: To study the prevalence of acute Q fever in Puducherry and surrounding districts of Tamil Nadu, India, employing a new commercial Real-Time Polymerase Chain Reaction (RT-PCR) kit and confirming it by the gold standard Immunofluorescence Assay (IFA)...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29195496/-does-the-salmonella-genomic-island-1-sgi1-confer-invasiveness-properties-to-human-isolates
#12
Claire de Curraize, Lucie Amoureux, Julien Bador, Angélique Chapuis, Eliane Siebor, Claire Clément, Juliette Sauge, Ludwig-Serge Aho-Glélé, Catherine Neuwirth
BACKGROUND: In the eighties, a multidrug resistant clone of Salmonella Typhimurium DT104 emerged in UK and disseminated worldwide. This clone harbored a Salmonella genomic island 1 (SGI1) that consists of a backbone and a multidrug resistant region encoding for penta-resistance (ampicillin, chloramphenicol/florfenicol, streptomycin/spectinomycin, sulphonamides and tetracycline (ACSSuT)). Several authors suggested that SGI1 might have a potential role in enhancement of virulence properties of Salmonella enterica...
December 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29188067/isolation-and-characterization-of-leptospira-interrogans-serovar-copenhageni-from-a-dog-from-saint-kitts
#13
Christopher R Larson, Michelle Dennis, Rajeev V Nair, Alejandro Llanes, Andrea Peda, Shamara Welcome, Sreekumari Rajeev
Introduction. Leptospirosis is a zoonotic bacterial disease of global distribution affecting humans and animals. The initial phase of leptospirosis resembles many other febrile illness and due to its broad and biphasic clinical manifestations, selection and implementation of appropriate diagnostic tests can be challenging. Case presentation. This report describes a case investigation of a 14 weeks old male, orphan puppy, presented with generalised jaundice, anemia, weakness, and anorexia. Clinical abnormalities included the evidence of renal and hepatic failure...
October 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29188050/effects-of-household-washing-on-bacterial-load-and-removal-of-escherichia-coli-from-lettuce-and-ready-to-eat-salads
#14
Elisabeth Uhlig, Crister Olsson, Jiayi He, Therese Stark, Zuzanna Sadowska, Göran Molin, Siv Ahrné, Beatrix Alsanius, Åsa Håkansson
Customer demands for fresh salads are increasing, but leafy green vegetables have also been linked to food-borne illness due to pathogens such as Escherichia coli O157:H7. As a safety measure, consumers often wash leafy vegetables in water before consumption. In this study, we analyzed the efficiency of household washing to reduce the bacterial content. Romaine lettuce and ready-to-eat mixed salad were washed several times in flowing water at different rates and by immersing the leaves in water. Lettuce was also inoculated with E...
November 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/29161941/are-we-missing-the-staphylococcus-aureus-bacteraemia-forest-for-the-mrsa-trees
#15
Kevin B Laupland
In this edition of the journal, Jokinen et al. report on a population based study of Staphylococcus aureus bacteraemia (SAB) in Finland. They observe increasing incidence of cases over time that are associated with an increase in blood culture sampling. As compared to methicillin-sensitive SAB that increases during the study period, the incidence of methicillin-resistant SAB increases and then decreases. The proportion of cases with penicillin-sensitive SAB markedly increases in the study representing 43% of isolates in the most recent year of surveillance...
November 21, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/29153459/-intrafamilial-disruptive-behaviour-disorders-in-children-and-adolescents-present-data-and-treatment-prospects
#16
E Bousquet, N Franc, C Ha, D Purper-Ouakil
Child-to-parent violence is a form of family violence that is still a well-kept secret. Abused parents can be victims of different types of abuse. Children can use both physical and psychological violence such as financial threats to take control of the home. In this situation, parents often no longer dare to contradict their children by fear of triggering uncontrollable violence. Although the phenomenon recently drew the attention of the media, there is still little knowledge about its prevalence and clinical characteristics...
November 16, 2017: L'Encéphale
https://www.readbyqxmd.com/read/29132413/germacranolide-type-sesquiterpene-lactones-from-smallanthus-sonchifolius-with-promising-activity-against-leishmania-mexicana-and-trypanosoma-cruzi
#17
Jerónimo L Ulloa, Renata Spina, Agustina Casasco, Patricia B Petray, Virginia Martino, Miguel A Sosa, Fernanda M Frank, Liliana V Muschietti
BACKGROUND: Leishmaniasis and Chagas disease are life-threatening illnesses caused by the protozoan parasites Leishmania spp. and Trypanosoma cruzi, respectively. They are known as "neglected diseases" due to the lack of effective drug treatments and the scarcity of research work devoted to them. Therefore, the development of novel and effective drugs is an important and urgent need. Natural products are an important source of bioactive molecules for the development of new drugs. In this study, we evaluated the activity of enhydrin, uvedalin and polymatin B, three sesquiterpene lactones (STLs) isolated from Smallanthus sonchifolius, on Leishmania mexicana (MNYC/BZ/62/M) and Trypanosoma cruzi (Dm28c)...
November 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29107574/high-prevalence-of-salmonella-spp-in-wastewater-reused-for-irrigation-assessed-by-molecular-methods
#18
Paula Santiago, Ana Jiménez-Belenguer, Jorge García-Hernández, Rosa Montes Estellés, Manuel Hernández Pérez, M Angeles Castillo López, María Antonia Ferrús, Yolanda Moreno
Salmonella spp. is one of the most important causal agents of food-borne illness in developed countries and its presence in irrigation water poses a risk to public health. Its detection in environmental samples is not easy when culture methods are used, and molecular techniques such as PCR or ribosomal rRNA probe hybridization (Fluorescent in situ Hybridization, FISH) are outstanding alternatives. The aim of this work was to determine the environmental risk due to the presence of Salmonella spp. in wastewater by culture, PCR and FISH...
January 2018: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29102764/treatment-of-malaria-and-related-symptoms-using-traditional-herbal-medicine-in-ethiopia
#19
Sultan Suleman, Takele Beyene Tufa, Dereje Kebebe, Sileshi Belew, Yimer Mekonnen, Fanta Gashe, Seid Musa, Evelien Wynendaele, Luc Duchateau, Bart De Spiegeleer
ETHNOPHARMACOLOGICAL RELEVANCE: Medicinal plants have always been an integral part of different cultures in Ethiopia in the treatment of different illnesses including malaria and related symptoms. However, due to lack of proper documentation, urbanization, drought, acculturation and deforestation, there is an increased risk of losing this traditional knowledge. Hence, the use of the indigenous knowledge should be well documented and validated for potential future use. AIM OF THE STUDY: To gather and document information on medicinal plants which are used in the traditional treatment of malaria and related symptoms in Ethiopia...
November 1, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29056694/inquiring-into-the-gaps-of-campylobacter-surveillance-methods
#20
REVIEW
Maria Magana, Stylianos Chatzipanagiotou, Angeliki R Burriel, Anastasios Ioannidis
Campylobacter is one of the most common pathogen-related causes of diarrheal illnesses globally and has been recognized as a significant factor of human disease for more than three decades. Molecular typing techniques and their combinations have allowed for species identification among members of the Campylobacter genus with good resolution, but the same tools usually fail to proceed to subtyping of closely related species due to high sequence similarity. This problem is exacerbated by the demanding conditions for isolation and detection from the human, animal or water samples as well as due to the difficulties during laboratory maintenance and long-term storage of the isolates...
July 19, 2017: Veterinary Sciences
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