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https://www.readbyqxmd.com/read/28444420/periportal-lymphatic-system-on-post-hepatobiliary-phase-gd-eob-dtpa-enhanced-mr-imaging-in-normal-subjects-and-patients-with-chronic-hepatitis-c
#1
Yasunari Yamada, Shunro Matsumoto, Hiromu Mori, Ryo Takaji, Maki Kiyonaga, Naoki Hijiya, Rika Tanoue, Kenichiro Tomonari, Shuichi Tanoue, Norio Hongo, Masayuki Ohta, Masataka Seike, Masafumi Inomata, Kazunari Murakami, Masatsugu Moriyama
PURPOSE: We sought to evaluate visualization of periportal lymphatics and lymph nodes (lymphatic system) on Gd-EOB-DTPA-enhanced magnetic resonance (MR) images using a fat-suppressed T2-weighted sequence with 3-dimensional (3D) volume isotropic turbo spin echo acquisition (VISTA) at 3.0 T in normal subjects and patients with chronic hepatitis C. METHODS: MR imaging was performed in 254 subjects between June 2013 and May 2016. After applying inclusion and exclusion criteria, the final population was 31 normal subjects and 34 patients with chronic hepatitis C...
April 25, 2017: Abdominal Radiology
https://www.readbyqxmd.com/read/28441599/transport-and-attenuation-of-salmonella-enterica-fecal-indicator-bacteria-and-a-poultry-litter-marker-gene-are-correlated-in-soil-columns
#2
Sirisha Mantha, Angela Anderson, Saraswati Poudel Acharya, Valerie J Harwood, Jennifer Weidhaas
Millions of tons of fecal-contaminated poultry litter are applied to U.S. agricultural fields annually. Precipitation and irrigation facilitate transport of fecal-derived pathogens and fecal indicator bacteria (FIB) to groundwater. The goal of this study was to compare transport of pathogens, FIB, and a microbial source tracking marker gene for poultry litter (LA35) in a simulated soil-to-groundwater system. Nine laboratory soil columns containing four different soil types were used to evaluate microbial transport to groundwater via infiltration...
April 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28433878/effect-of-freshwater-sediment-characteristics-on-the-persistence-of-fecal-indicator-bacteria-and-genetic-markers-within-a-southern-california-watershed
#3
Amity G Zimmer-Faust, Vanessa Thulsiraj, Catalina Marambio-Jones, Yiping Cao, John F Griffith, Patricia A Holden, Jennifer A Jay
In this study, the aging of culturable FIB and DNA representing genetic markers for Enterococcus spp. (ENT1A), general Bacteroides (GB3), and human-associated Bacteroides (HF183) in freshwater sediments was evaluated. Freshwater sediment was collected from four different sites within the upper and lower reach of the Topanga Creek Watershed and two additional comparator sites within the Santa Monica Bay, for a total of six sites. Untreated (ambient) and oven-dried (reduced microbiota) sediment was inoculated with 5% sewage and artificial freshwater...
April 18, 2017: Water Research
https://www.readbyqxmd.com/read/28425016/a-simple-bedside-blood-test-fibrofast-fib-5-is-superior-to-fib-4-index-for-the-differentiation-between-non-significant-and-significant-fibrosis-in-patients-with-chronic-hepatitis-c
#4
G Shiha, S Seif, A Eldesoky, M Elbasiony, R Soliman, A Metwally, K Zalata, N Mikhail
INTRODUCTION: A simple non-invasive score (Fibrofast, FIB-5) was developed using five routine laboratory tests (ALT, AST, alkaline phosphatase, albumin and platelets count) for the detection of significant hepatic fibrosis in patients with chronic hepatitis C. The FIB-4 index is a non-invasive test for the assessment of liver fibrosis, and a score of ≤1.45 enables the correct identification of patients who have non-significant (F0-1) from significant fibrosis (F2-4), and could avoid liver biopsy...
April 19, 2017: Hepatology International
https://www.readbyqxmd.com/read/28422310/adult-onset-cystic-fibrosis-liver-disease-diagnosis-and-characterization-of-an-underappreciated-entity
#5
Christopher Koh, Sasan Sakiani, Pallavi Surana, Xiongce Zhao, Jason Eccleston, David E Kleiner, David Herion, T Jake Liang, Jay H Hoofnagle, Milica Chernick, Theo Heller
BACKGROUND & AIMS: Cystic fibrosis liver disease (CFLD), a leading cause of death in cystic fibrosis (CF), is mostly described in pediatric populations. Adult-onset CFLD lacks sufficient characterization and diagnostic tools. METHODS: A cohort of CF patients without CFLD during childhood were followed for up to 38 years with serologic testing, imaging, and noninvasive fibrosis markers. Historical CFLD diagnostic criteria were compared with a newly proposed CFLD criteria...
April 19, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28414057/non-invasive-assessment-of-liver-fibrosis-in-patients-with-hbv-related-chronic-liver-disease-undergoing-antiviral-treatment-a-preliminary-study
#6
Cristina Stasi, Elena Salomoni, Umberto Arena, Giampaolo Corti, Paolo Montalto, Filippo Bartalesi, Fabio Marra, Giacomo Laffi, Stefano Milani, Anna Linda Zignego, Massimo Pinzani
In chronic hepatitis B (CHB) patients, fibrosis assessment during antiviral treatment is a key step in the clinical management. Aim of this study was to evaluate the performance of elastography in assessing fibrosis stage in CHB before and after two years of nucleoside/nucleotide analogues (NUC) treatment in comparison with indirect serum markers. CHB diagnosis was made according to standard criteria. A clinical and virological evaluation was performed at baseline and again at 3, 6, 9, 12 18, and 24 months during treatment...
April 13, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28413495/perioperative-application-of-salvianolate-on-oxidative-stress-and-plasma-imd-adm2-in-patients-with-acute-myocardial-infarction-undergoing-pci
#7
Qiang Zhang, Fengjun Jiao, Chongjun Hua
The present study was conducted to investigate the effects of salvianolate on perioperative oxidative stress and plasma Intermedin/adrenomedullin 2 (IMD/ADM2) in patients with acute myocardial infarction (AMI) undergoing percutaneous coronary intervention (PCI). The clinical data of 108 patients with AMI treated by PCI were retrospectively analyzed. According to the use of salvianolate in perioperative period, the patients were divided into the study group (n=62) and the control group (n=46). The control group was given routine treatment while the study group was given routine treatment combined with intravenous infusion of salvianolate (200 mg administered once at 24 h before operation, once/day after operation, 1 week later, the curative effect was observed)...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28403096/the-efficiency-and-safety-of-fascia-iliaca-block-for-pain-control-after-total-joint-arthroplasty-a-meta-analysis
#8
Peng Zhang, Jifeng Li, Yuze Song, Xiao Wang
BACKGROUND: This meta-analysis aimed to perform a meta-analysis including randomized controlled trials (RCTs) to assess the efficiency and safety of fascia iliaca block (FIB) for pain control in patients undergoing total joint arthroplasty (TJA). METHODS: A systematic search was performed in Medline (1966-2017.03), PubMed (1966-2017.03), Embase (1980-2017.03), ScienceDirect (1985-2017.03) and the Cochrane Library. Study evaluated the efficiency and safety of FIB in TJA was selected...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28394122/embedding-free-method-for-preparation-of-cross-sections-of-organic-materials-for-micro-chemical-analysis-using-gas-cluster-ion-beam-sputtering
#9
Ichiro Mihara, Rasmus Havelund, Ian S Gilmore
We present a novel in situ mask method for the preparation of cross-sections of organic materials such as polymer multilayer films suitable for chemical imaging of buried interfaces. We demonstrate this method on a model buried interface system consisting of a piece of Scotch tape adhered to a PET substrate and a protective film used in consumer packaging. A high dose of gallium from a focused ion beam (FIB) was used to produce a damaged overlayer on the surface of the organic sample. The damaged layer has a significantly slower sputter rate compared to the native undamaged organic material...
April 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28384541/focused-ion-beam-assisted-fabrication-of-soft-high-aspect-ratio-silicon-nanowire-atomic-force-microscopy-probes
#10
Peter Knittel, Nicolas Hibst, Boris Mizaikoff, Steffen Strehle, Christine Kranz
In this study, high-aspect ratio silicon nanowire (SiNW) - modified atomic force microscopy (AFM) probes are fabricated using focused ion beam (FIB) microfabrication technology and vapor-solid-solid synthesis. Commercially available soft silicon nitride probes are used for localized nanowire growth yielding soft high-aspect ratio AFM probes. The SiNW-modified cantilevers are used here for imaging in PeakForce Tappingۛ (PFT) mode, which offers high force control along with valuable information about tip-sample adhesion...
March 28, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28383028/3d-lattice-distortions-and-defect-structures-in-ion-implanted-nano-crystals
#11
Felix Hofmann, Edmund Tarleton, Ross J Harder, Nicholas W Phillips, Pui-Wai Ma, Jesse N Clark, Ian K Robinson, Brian Abbey, Wenjun Liu, Christian E Beck
Focussed Ion Beam (FIB) milling is a mainstay of nano-scale machining. By manipulating a tightly focussed beam of energetic ions, often gallium (Ga(+)), FIB can sculpt nanostructures via localised sputtering. This ability to cut solid matter on the nano-scale revolutionised sample preparation across the life, earth and materials sciences. Despite its widespread usage, detailed understanding of the FIB-induced structural damage, intrinsic to the technique, remains elusive. Here we examine the defects caused by FIB in initially pristine objects...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28374799/investigation-of-ac-magnetic-field-stimulated-nanoelectroporation-of-magneto-electric-nano-drug-carrier-inside-cns-cells
#12
Ajeet Kaushik, Roozbeh Nikkhah-Moshaie, Raju Sinha, Vinay Bhardwaj, Venkata Atluri, Rahul Dev Jayant, Adriana Yndart, Babak Kateb, Nezih Pala, Madhavan Nair
In this research, we demonstrate cell uptake of magneto-electric nanoparticles (MENPs) through nanoelectroporation (NEP) using alternating current (ac)-magnetic field stimulation. Uptake of MENPs was confirmed using focused-ion-beam assisted transmission electron microscopy (FIB-TEM) and validated by a numerical simulation model. The NEP was performed in microglial (MG) brain cells, which are highly sensitive for neuro-viral infection and were selected as target for nano-neuro-therapeutics. When the ac-magnetic field optimized (60 Oe at 1 kHz), MENPs were taken up by MG cells without affecting cell health (viability > 92%)...
April 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28372387/note-nanomechanical-characterization-of-soft-materials-using-a-micro-machined-nanoforce-transducer-with-an-fib-made-pyramidal-tip
#13
Z Li, S Gao, U Brand, K Hiller, N Wollschläger, F Pohlenz
The quantitative nanomechanical characterization of soft materials using the nanoindentation tech-nique requires further improvements in the performances of instruments, including their force resolution in particular. A micro-machined silicon nanoforce transducer based upon electrostatic comb drives featuring the force and depth resolutions down to ∼1 nN and 0.2 nm, respectively, is described. At the end of the MEMS transducer's main shaft, a pyramidal tip is fabricated using a focused ion beam facility. A proof-of-principle setup with this MEMS nanoindenter has been established to measure the mechanical properties of soft polydimethylsiloxane...
March 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28370277/3d-electron-microscopic-characterization-of-interstitial-cells-in-the-human-bladder-upper-lamina-propria
#14
Jochen Neuhaus, Birgit Schröppel, Martin Dass, Hans Zimmermann, Hartwig Wolburg, Petra Fallier-Becker, Thomas Gevaert, Claus J Burkhardt, Hoang Minh Do, Jens-Uwe Stolzenburg
AIMS: To explore the ultrastructure of interstitial cells in the upper lamina propria of the human bladder, to describe the spatial relationships and to investigate cell-cell contacts. METHODS: Focused ion beam scanning electron microscopy (FIB-SEM), 3-View SEM and confocal laser scanning microscopy were used to analyze the 3D ultrastructure of the upper lamina propria in male and female human bladders. RESULTS: 3View-SEM image stacks as large as 59 × 59 × 17 μm(3) (xyz) at a resolution of 16 × 16 × 50 nm(3) and high resolution (5 × 5 × 10 nm(3) ) FIB-SEM stacks could be analyzed...
March 31, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/28369305/comparing-child-pugh-meld-and-fib-4-to-predict-clinical-outcomes-in-hcv-infected-persons-results-from-erchives
#15
Adeel A Butt, Yanjie Ren, Vincent Lo Re, Tamar Taddei, David Kaplan
Background: Identifying HCV+ persons at high risk of early complications can help prioritize treatment decisions. We conducted this study to compare Child-Turcotte-Pugh (CP), MELD and FIB-4 scores for predicting clinical outcomes and to identify those at low risk of complications. Methods: Within Electronically Retrieved Cohort of HCV-Infected Veterans, we identified HCV+ persons and excluded those with HIV+, HBsAg+, prevalent hepatic decompensation (HD), hepatocellular carcinoma (HCC) and those treated for HCV...
March 22, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28364653/genetic-characterization-of-fecal-impacts-of-seagull-migration-on-an-urban-scenery-lake
#16
Baolei Wu, Xiaochang C Wang, Mawuli Dzakpasu
A microbial source tracking scheme was devised to differentiate fecal impacts of seagulls from that of human activities on an urban scenery lake in southern China, which is a major wintering ground for the black-headed seagull. Fecal contamination of seagulls was characterized by quantifying a novel genetic marker targeting Catellicoccus marimamalium. Quantification of this marker was combined with those of Escherichia coli, human-associated Bacteroidales, thermophilic Campylobacter and Helicobacter. Findings of a year-round study indicate that C...
March 20, 2017: Water Research
https://www.readbyqxmd.com/read/28361795/native-extracellular-matrix-fibroin-hydrogels-for-adipose-tissue-engineering-with-enhanced-vascularization
#17
Alişan Kayabölen, Dilek Keskin, Andaç Aykan, Yıldırım Karslıoğlu, Fatih Zor, Aysen Tezcaner
Adipose tissue engineering is a promising field for regeneration of soft tissue defects. However, vascularization is needed since cells in thick implants cannot reach to nutrient and oxygen by diffusion. Obtaining a biocompatible scaffold with good mechanical properties is another problem. In this study, we aimed to develop thick and vascularized adipose tissue constructs supporting cell viability and adipose tissue regeneration. Hydrogels were prepared by mixing rat decellularized adipose tissue (DAT) and silk fibroin at different v/v ratios (3:1, 1:1 and 1:3) and vortexing...
March 31, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28347811/whole-blood-coagulation-assays-rotem-and-t-tas-to-monitor-dabigatran-treatment
#18
Viktor Taune, Håkan Wallén, Anna Ågren, Gunilla Gryfelt, Carolina Sjövik, Anna M Wintler, Rickard E Malmström, Agneta Wikman, Mika Skeppholm
BACKGROUND: A rapid and reliable assessment of the dabigatran effect is desirable in dabigatran treated patients with uncontrolled bleeding or before acute surgery. OBJECTIVE: To evaluate how the viscoelastic point-of-care test Rotational thromboelastometry (ROTEM) and Total Thrombus-formation system (T-TAS), which studies thrombus formation under flowing conditions, correlate with dabigatran concentrations in patients with atrial fibrillation (AF). METHOD: ROTEM using the reagents In-tem, Ex-tem, Fib-tem or low tissue factor concentration (TF), and T-TAS with the AR-chip (shear rate 600s(-1), representing flow in large arteries) were investigated in whole blood samples...
March 18, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28347203/thromboelastometric-assessment-of-hemostasis-in-newborn-piemontese-calves
#19
Antonio Borrelli, Angelica Botto, Cristiana Maurella, Sara Falco, Elena Pagani, Barbara Miniscalco, Alberto Tarducci, Barbara Bruno
We investigated possible age-related differences in coagulation profiles in bovine species by means of rotational thromboelastometric (ROTEM) analysis. We evaluated hemostasis by ROTEM in newborn Piemontese calves at birth (T0), 8 d (T8), and 15 d (T15) of age and compared the ROTEM results obtained in 16 newborn calves with 28 adult Piemontese cattle. Hemostasis was evaluated using standard coagulation tests and ROTEM analysis, obtaining in-TEM, ex-TEM, and fib-TEM profiles. Statistically significant differences in the ROTEM profiles of newborn calves were found between T0 and T8 and between T0 and T15 ( p < 0...
March 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28327151/quantification-of-silver-nanoparticle-uptake-and-distribution-within-individual-human-macrophages-by-fib-sem-slice-and-view
#20
Erik Guehrs, Michael Schneider, Christian M Günther, Piet Hessing, Karen Heitz, Doreen Wittke, Ana López-Serrano Oliver, Norbert Jakubowski, Johanna Plendl, Stefan Eisebitt, Andrea Haase
BACKGROUND: Quantification of nanoparticle (NP) uptake in cells or tissues is very important for safety assessment. Often, electron microscopy based approaches are used for this purpose, which allow imaging at very high resolution. However, precise quantification of NP numbers in cells and tissues remains challenging. The aim of this study was to present a novel approach, that combines precise quantification of NPs in individual cells together with high resolution imaging of their intracellular distribution based on focused ion beam/ scanning electron microscopy (FIB/SEM) slice and view approaches...
March 21, 2017: Journal of Nanobiotechnology
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