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https://www.readbyqxmd.com/read/28094314/reassessment-of-rebleeding-risk-of-forrest-ib-oozing-peptic-ulcer-bleeding-in-a-large-international-randomized-trial
#1
Dennis M Jensen, Stefan Eklund, Tore Persson, Henrik Ahlbom, Robert Stuart, Alan N Barkun, Ernest J Kuipers, Joachim Mössner, James Y Lau, Joseph J Sung, Jan Kilhamn, Tore Lind
OBJECTIVES: Our aims were to assess risks of early rebleeding after successful endoscopic hemostasis for Forrest oozing (FIB) peptic ulcer bleeding (PUBs) compared with other stigmata of recent hemorrhage (SRH). METHODS: These were post hoc multivariable analyses of a large, international, double-blind study (NCT00251979) of patients randomized to high-dose intravenous (IV) esomeprazole (PPI) or placebo for 72 h. Rebleeding rates of patients with PUB SRH treated with either PPI or placebo after successful endoscopic hemostasis were also compared...
January 17, 2017: American Journal of Gastroenterology
https://www.readbyqxmd.com/read/28077090/gbs-based-single-dosage-markers-for-linkage-and-qtl-mapping-allow-gene-mining-for-yield-related-traits-in-sugarcane
#2
Thiago Willian Almeida Balsalobre, Guilherme da Silva Pereira, Gabriel Rodrigues Alves Margarido, Rodrigo Gazaffi, Fernanda Zatti Barreto, Carina Oliveira Anoni, Cláudio Benício Cardoso-Silva, Estela Araújo Costa, Melina Cristina Mancini, Hermann Paulo Hoffmann, Anete Pereira de Souza, Antonio Augusto Franco Garcia, Monalisa Sampaio Carneiro
BACKGROUND: Sugarcane (Saccharum spp.) is predominantly an autopolyploid plant with a variable ploidy level, frequent aneuploidy and a large genome that hampers investigation of its organization. Genetic architecture studies are important for identifying genomic regions associated with traits of interest. However, due to the genetic complexity of sugarcane, the practical applications of genomic tools have been notably delayed in this crop, in contrast to other crops that have already advanced to marker-assisted selection (MAS) and genomic selection...
January 11, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28070899/sofosbuvir-based-treatment-regimens-real-life-results-of-14-409-chronic-hcv-genotype-4-patients-in-egypt
#3
A Elsharkawy, R Fouad, W El Akel, M El Raziky, M Hassany, G Shiha, M Said, I Motawea, T El Demerdash, S Seif, A Gaballah, Y El Shazly, M A M Makhlouf, I Waked, A O Abdelaziz, A Yosry, M El Serafy, M Thursz, W Doss, G Esmat
BACKGROUND: Chronic hepatitis C virus infection is one of the most important health problems in Egypt. The Ministry of Health's National Treatment Programme introduced sofosbuvir-based therapy in October 2014. AIM: To assess the clinical effectiveness and predictors of response to SOF-based treatment regimens, either dual therapy, with SOF/ribavirin (RBV) for 6 months or triple therapy with SOF/peg-IFN-alfa-2a/RBV for 3 months, in a cohort of patients treated in National Treatment Programme affiliated centres in Egypt...
January 9, 2017: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28070199/performance-and-utility-of-transient-elastography-and-non-invasive-markers-of-liver-fiibrosis-in-patients-with-autoimmune-hepatitis-a-single-centre-experience
#4
Olympia E Anastasiou, Matthias Büchter, Hideo A Baba, Johannes Korth, Ali Canbay, Guido Gerken, Alisan Kahraman
OBJECTIVES: Autoimmune hepatitis (AIH) is a relatively rare cause of hepatic dysfunction, which can lead to acute liver failure (ALV) and cirrhosis if not treated. The performance of transient elastography (TE) compared to liver biopsy has been evaluated in many liver diseases. The aim of the present study was to evaluate the performance of TE and other non-invasive markers for liver fiibrosis in patients with biopsy-proven AIH. METHODS: Fifty-three patients who were treated at the department of gastroenterology and hepatology of the University Clinic Essen from 2008 to 2013 included in this retrospective study...
November 2016: Hepatitis Monthly
https://www.readbyqxmd.com/read/28067683/acoustic-radiation-force-impulse-elastography-comparison-and-combination-with-other-noninvasive-tests-for-the-diagnosis-of-compensated-liver-cirrhosis
#5
Lukas Pfeifer, Werner Adler, Steffen Zopf, Jürgen Siebler, Dane Wildner, Ruediger S Goertz, Barbara Schellhaas, Markus F Neurath, Deike Strobel
BACKGROUND AND AIMS: The aim of this study was to compare acoustic radiation force impulse (ARFI) elastography with other noninvasive tests and to develop a new score for the assessment of liver fibrosis/cirrhosis. MATERIALS AND METHODS: B-mode ultrasound (including high-frequency liver surface evaluation), routine blood tests, ARFI quantification, and mini-laparoscopic liver evaluation were obtained in compensated patients scheduled for mini-laparoscopic biopsy...
January 6, 2017: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28062041/improved-force-spectroscopy-using-focused-ion-beam-modified-cantilevers
#6
J K Faulk, D T Edwards, M S Bull, T T Perkins
Atomic force microscopy (AFM) is widely used in biophysics, including force-spectroscopy studies of protein folding and protein-ligand interactions. The precision of such studies increases with improvements in the underlying quality of the data. Currently, data quality is limited by the mechanical properties of the cantilever when using a modern commercial AFM. The key tradeoff is force stability vs short-term force precision and temporal resolution. Here, we present a method that avoids this compromise: efficient focused-ion-beam (FIB) modification of commercially available cantilevers...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28060754/diagnostic-value-of-fib-4-for-liver-fibrosis-in-patients-with-hepatitis-b-a-meta-analysis-of-diagnostic-test
#7
Zhi Yin, Jin Zou, Qiongxuan Li, Lizhang Chen
This study is aimed at evaluating the diagnostic value of FIB-4 for liver fibrosis in patients with hepatitis B through a meta-analysis of diagnostic test. We conducted a comprehensive search in the Pubmed, Embase, Web of Science, and Chinese National Knowledge Infrastructure before October 31, 2016. Stata 14.0 software was used for calculation and statistical analyses. We used the sensitivity, specificity, positive and negative likelihood ratio (PLR, NLR), diagnostic odds ratio (DOR) and 95% confidence intervals (CIs) to evaluate the diagnostic value of FIB-4 for liver fibrosis in patients with hepatitis B...
January 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28054154/interstitial-cell-modulation-of-pyeloureteric-peristalsis-in-the-mouse-renal-pelvis-examined-using-fibsem-tomography-and-calcium-indicators
#8
Hikaru Hashitani, Michael J Nguyen, Haruka Noda, Retsu Mitsui, Ryuhei Higashi, Keisuke Ohta, Kei-Ichiro Nakamura, Richard J Lang
Typical and atypical smooth muscle cells (TSMCs and ASMCs, respectively) and interstitial cells (ICs) within the pacemaker region of the mouse renal pelvis were examined using focused ion beam scanning electron (FIB SEM) tomography, immunohistochemistry and Ca(2+) imaging. Individual cells within 500-900 electron micrograph stacks were volume rendered and associations with their neighbours established. 'Ribbon-shaped', Ano1 Cl(-) channel immuno-reactive ICs were present in the adventitia and the sub-urothelial space adjacent to the TSMC layer...
January 4, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28040888/a-seasonal-assessment-of-opportunistic-premise-plumbing-pathogens-in-roof-harvested-rainwater-tanks
#9
Kerry A Hamilton, Warish Ahmed, Andrew Palmer, Kylie Smith, Simon Toze, Charles N Haas
A seasonal study on the occurrence of six opportunistic premise plumbing pathogens (OPPPs) in 24 roof-harvested rainwater (RHRW) tanks repeatedly sampled over six monthly sampling events (n = 144) from August 2015 to March 2016 was conducted using quantitative qPCR. Fecal indicator bacteria (FIB) Escherichia coli (E. coli) and Enterococcus spp. were enumerated using culture-based methods. All tank water samples over the six events were positive for at least one OPPP (Legionella spp., Legionella pneumophila, Mycobacterium avium, Mycobacterium intracellulare, Pseudmonas aeruginosa, or Acanthamoeba spp...
January 2, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28035087/fib-synthesis-of-bi2se3-1d-nanowires-demonstrating-the-co-existence-of-shubnikov-de-haas-oscillations-and-linear-magnetoresistance
#10
Biplab Bhattacharyya, Alka Sharma, V P S Awana, T D Senguttuvan, Sudhir Husale
Since the discovery of topological insulators (TIs), there are considerable interests in demonstrating metallic surface states (SS), their shielded robust nature to the backscattering and study their properties at nanoscale dimensions by fabricating nanodevices. Here we address an important scientific issue related to TI whether one can clearly demonstrate the robustness of topological surface states (TSS) to the presence of disorder that does not break any fundamental symmetry. The simple straightforward method of FIB milling was used to synthesize nanowires of Bi2Se3 which we believe is an interesting route to test robustness of TSS and the obtained results are new compared to many of the earlier papers on quantum transport in TI demonstrating the robustness of metallic SS to gallium (Ga) doping...
February 22, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28003795/the-effect-of-size-on-the-strength-of-fcc-metals-at-elevated-temperatures-annealed-copper
#11
Jeffrey M Wheeler, Christoph Kirchlechner, Jean-Sébastien Micha, Johann Michler, Daniel Kiener
As the length scale of sample dimensions is reduced to the micron and sub-micron scales, the strength of various materials has been observed to increase with decreasing size, a fact commonly referred to as the 'sample size effect'. In this work, the influence of temperature on the sample size effect in copper is investigated using in situ microcompression testing at 25, 200 and 400 °C in the SEM on vacuum-annealed copper structures, and the resulting deformed structures were analysed using X-ray μLaue diffraction and scanning electron microscopy...
December 1, 2016: Philosophical Magazine (Abingdon, England)
https://www.readbyqxmd.com/read/28002184/prevalence-and-predictors-of-hospitalizations-among-hiv-infected-and-at-risk-hiv-uninfected-women
#12
Anna L Hotton, Kathleen M Weber, Ronald C Hershow, Kathryn Anastos, Peter Bacchetti, Elizabeth T Golub, Deborah Gustafson, Alexandra M Levine, Mary Young, Mardge H Cohen
OBJECTIVES: We evaluated the Veterans Aging Cohort Study (VACS) Index score, an index composed of age, CD4 count, viral load, hemoglobin, Hepatitis C (HCV) co-infection, Fibrosis Index-4 (FIB-4), and estimated glomerular filtration rate (EGFR), and psychosocial and clinical risk factors for all-cause hospitalization among HIV-infected women on HAART and HIV-uninfected women. METHODS: Data were collected from 2008 - 2014 from 1585 HAART-experienced HIV-infected and 692 uninfected women...
December 17, 2016: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/27957576/3d-multi-energy-deconvolution-electron-microscopy
#13
Michiel de Goede, Eric Johlin, Beniamino Sciacca, Faysal Boughorbel, Erik C Garnett
Three-dimensional (3D) characterization of nanomaterials is traditionally performed by either cross-sectional milling with a focused ion beam (FIB), or transmission electron microscope tomography. While these techniques can produce high quality reconstructions, they are destructive, or require thin samples, often suspended on support membranes. Here, we demonstrate a complementary technique allowing non-destructive investigation of the 3D structure of samples on bulk substrates. This is performed by imaging backscattered electron (BSE) emission at multiple primary beam energies - as the penetration depth of primary electrons is proportional to the beam energy, depth information can be obtained through variations in the beam acceleration...
December 13, 2016: Nanoscale
https://www.readbyqxmd.com/read/27955711/hcv-co-infection-and-markers-of-liver-injury-and-fibrosis-among-hiv-positive-childbearing-women-in-ukraine-results-from-a-cohort-study
#14
Heather Bailey, Nataliya Nizova, Violeta Martsynovska, Alla Volokha, Ruslan Malyuta, Mario Cortina-Borja, Claire Thorne
BACKGROUND: Ukraine's injecting drug use-driven HIV epidemic is among the most severe in Europe with high burden of HCV co-infection. HIV/HCV co-infected individuals are at elevated risk of HCV-related morbidity, but little is known about burden of liver disease and associated factors in the HIV-positive population in Ukraine, particularly among women. METHODS: Characteristics of 2050 HIV-positive women enrolled into the Ukrainian Study of HIV-infected Childbearing Women were described by HCV serostatus...
December 12, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27935960/hydrological-regime-and-water-shortage-as-drivers-of-the-seasonal-incidence-of-diarrheal-diseases-in-a-tropical-montane-environment
#15
Laurie Boithias, Marc Choisy, Noy Souliyaseng, Marine Jourdren, Fabrice Quet, Yves Buisson, Chanthamousone Thammahacksa, Norbert Silvera, Keooudone Latsachack, Oloth Sengtaheuanghoung, Alain Pierret, Emma Rochelle-Newall, Sylvia Becerra, Olivier Ribolzi
BACKGROUND: The global burden of diarrhea is a leading cause of morbidity and mortality worldwide. In montane areas of South-East Asia such as northern Laos, recent changes in land use have induced increased runoff, soil erosion and in-stream suspended sediment loads, and potential pathogen dissemination. To our knowledge, few studies have related diarrhea incidences to catchment scale hydrological factors such as river discharge, and loads of suspended sediment and of Fecal Indicator Bacteria (FIB) such as Escherichia coli, together with sociological factors such as hygiene practices...
December 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27935164/kaolin-activated-thromboelastography-and-standard-coagulation-assays-in-cyanotic-and-acyanotic-infants-undergoing-complex-cardiac-surgery-a-prospective-cohort-study
#16
Alessandra Rizza, Zaccaria Ricci, Chiara Pezzella, Isabella Favia, Giovina Di Felice, Marco Ranucci, Paola Cogo
BACKGROUND AND AIMS: Several studies report the use of thromboelatography (TEG) to monitor coagulation in pediatric cardiac surgery. The aim of this study was to compare baseline and intraoperative TEG, TEG-functional fibrinogen, and standard coagulation assays in children with cyanotic and acyanotic congenital heart disease (CHD) undergoing cardiac surgery. METHODS: This is a prospective observational study of 63 children aged <24 months undergoing cardiac surgery with cardiopulmonary bypass (CPB)...
December 9, 2016: Paediatric Anaesthesia
https://www.readbyqxmd.com/read/27934775/real-world-effectiveness-and-predictors-of-sustained-virological-response-with-all-oral-therapy-in-21-242-hepatitis-c-genotype-1-patients
#17
Lisa I Backus, Pamela S Belperio, Troy A Shahoumian, Timothy P Loomis, Larry A Mole
BACKGROUND: Predictors of SVR to all-oral hepatitis C virus (HCV) regimens can inform nuanced treatment decisions. We evaluated effectiveness and identified predictors of SVR for ledipasvir/sofosbuvir±ribavirin (LDV/SOF±RBV) and ombitasvir/paritaprevir/ritonavir+dasabuvir (OPrD)±RBV in patients treated in routine practice. METHODS: Observational, intent-to-treat cohort of 21,142 genotype 1 patients initiating 8 or 12 weeks of LDV/SOF±RBV or 12 weeks of OPrD±RBV at any Veterans Affairs facility...
December 9, 2016: Antiviral Therapy
https://www.readbyqxmd.com/read/27926787/comparison-of-the-effects-of-a-balanced-crystalloid-based-and-a-saline-based-tetrastarch-solution-on-canine-whole-blood-coagulation-and-platelet-function
#18
Annina Reuteler, Shannon Axiak-Flammer, Judith Howard, Katja-Nicole Adamik
OBJECTIVE: To evaluate the effects of a 6% hydroxyethyl starch (130/0.42) solution in either a buffered, electrolyte-balanced (HES-BAL), or a saline (HES-SAL) carrier solution on canine platelet function and whole blood coagulation. DESIGN: Prospective, randomized study. SETTING: University teaching hospital. ANIMALS: Thirty-seven client-owned dogs undergoing general anesthesia for arthroscopy or imaging studies. INTERVENTIONS: Dogs received a 15 mL/kg intravenous bolus of HES-SAL (n = 13), HES-BAL (n = 14), or a modified Ringer's solution (n = 10) over 30-40 minutes...
December 7, 2016: Journal of Veterinary Emergency and Critical Care
https://www.readbyqxmd.com/read/27918627/exploring-dynamic-surface-processes-during-silicate-mineral-wollastonite-dissolution-with-liquid-cell-tem
#19
D N Leonard, R Hellmann
Most liquid cell transmission electron microscopy (LC TEM) studies focus on nanoparticles or nanowires, in large part because the preparation and study of materials in this size range is straightforward. By contrast, this is not true for samples in the micrometre size range, in large part because of the difficulties associated with sample preparation starting from a 'bulk' material. There are also many advantages inherent to the study of micrometre-sized samples compared to their nanometre-sized counterparts...
December 5, 2016: Journal of Microscopy
https://www.readbyqxmd.com/read/27869059/site-specific-preparation-of-intact-solid-liquid-interfaces-by-label-free-in-situ-localization-and-cryo-focused-ion-beam-lift-out
#20
Michael J Zachman, Emily Asenath-Smith, Lara A Estroff, Lena F Kourkoutis
Scanning transmission electron microscopy (STEM) allows atomic scale characterization of solid-solid interfaces, but has seen limited applications to solid-liquid interfaces due to the volatility of liquids in the microscope vacuum. Although cryo-electron microscopy is routinely used to characterize hydrated samples stabilized by rapid freezing, sample thinning is required to access the internal interfaces of thicker specimens. Here, we adapt cryo-focused ion beam (FIB) "lift-out," a technique recently developed for biological specimens, to prepare intact internal solid-liquid interfaces for high-resolution structural and chemical analysis by cryo-STEM...
December 2016: Microscopy and Microanalysis
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