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https://www.readbyqxmd.com/read/28643098/-importance-of-wound-irrigation-solutions-and-fluids-with-antiseptic-effects-in-therapy-and-prophylaxis-update-2017
#1
REVIEW
Christian Willy, Catharina Scheuermann-Poley, Marcus Stichling, Thomas von Stein, Axel Kramer
INTRODUCTION: Despite the use of modern antibiotics as well as complex perioperative, intraoperative and postoperative prophylactic measures, the rate of surgical site infections (SSI) could not be significantly reduced. The introduction of biocompatible antiseptic drugs with a high microbiocidal effect provided a successful alternative for infection prevention and therapy, particularly in a time of increasing occurrence of multi-drug resistant pathogens. Hence, questions about the risk-benefit ratio of antiseptic wound irrigation solutions, the prophylactic use of wound irrigation solutions for the reduction of SSI and the effect of negative pressure wound therapy combined with instillation (NPWTi) need to be answered...
June 22, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/28642925/the-effect-of-gelatinase-production-of-enterococcus-faecalis-on-adhesion-to-dentin-after-irrigation-with-various-endodontic-irrigants
#2
Mehmet Burak Guneser, Ayce Unverdi Eldeniz
Objectives: The aim of this study was to evaluate whether the gelatinase production ability of Enterococcus faecalis provides any advantage on adhesion of this bacterium to dentin treated with various irrigants and their combinations. Materials and methods: Standardized dentin discs were randomly divided into five groups (n = 20): group 1: 2.5% sodium hypochlorite (NaOCl), group 2: 2% chlorhexidine (CHX), group 3: NaOCl + Saline + CHX, group 4: NaOCl + EDTA + NaOCl, group 5: QMix. After incubation of dentin discs with irrigants, each group was divided into two subgroups (n = 10) according to the bacterial strains used; a gelatinase-producing and a gelatinase-deficient strain of E...
December 2016: Acta Biomaterialia Odontologica Scandinavica
https://www.readbyqxmd.com/read/28633052/disinfection-efficiencies-of-sage-and-spearmint-essential-oils-against-planktonic-and-biofilm-staphylococcus-aureus-cells-in-comparison-with-sodium-hypochlorite
#3
Dimitrios Vetas, Eleni Dimitropoulou, Gregoria Mitropoulou, Yiannis Kourkoutas, Efstathios Giaouris
Staphylococcus aureus causes human infections and foodborne intoxications. This study explored the potential antibacterial actions of sage and spearmint essential oils (EOs) against both its planktonic and biofilm cells, in comparison with sodium hypochlorite (NaOCl), a commonly applied chemical sanitizer. Initially, the minimum inhibitory and bactericidal concentrations (MICs, MBCs) of each plant mixture were determined against planktonic cultures, following growth at 30°C for 24h. Stationary phase planktonic bacteria were then individually exposed for 6min to either each EO (applied at 1-2×MBC; 2...
June 8, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28631818/inactivation-of-bacillus-cereus-spores-on-red-chili-peppers-using-a-combined-treatment-of-aqueous-chlorine-dioxide-and-hot-air-drying
#4
Songyi Kim, Huyong Lee, Jee-Hoon Ryu, Hoikyung Kim
The effect of a combined treatment using aqueous chlorine dioxide (ClO2 ) and hot-air drying to inactivate Bacillus cereus spores on red chili peppers was evaluated. Ten washed and dried pepper samples, each comprising half of a single pepper (Capsicum annuum L.), were inoculated with B. cereus spore suspension. The inoculated samples were washed with sodium hypochlorite (NaOCl; 50, 100, or 200 μg/mL) or ClO2 (50, 100, or 200 μg/mL) solution for 1 min and then air-dried (25 ± 1 °C, 47 ± 1% relative humidity), which was followed by drying with hot air at 55 °C for up to 48 h...
June 20, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28629970/variation-analysis-of-bacterial-polyhydroxyalkanoates-production-using-saturated-and-unsaturated-hydrocarbons
#5
Saiqa Tufail, Sajida Munir, Nazia Jamil
Polyhydroxyalkanoates (PHA) are efficient, renewable and environment friendly polymeric esters. These polymers are synthesized by a variety of microbes under stress conditions. This study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. Six bacterial strains were isolated and identified as Bacillus cereus (KF270349), Klebsiella pneumoniae (KF270350), Bacillus subtilis (KF270351), Brevibacterium halotolerance (KF270352), Pseudomonas aeruginosa (KF270353), and Stenotrophomonas rhizoposid (KF270354) by ribotyping...
May 29, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28628976/effective-enzymatic-in-situ-saccharification-of-bamboo-shoot-shell-pretreated-by-dilute-alkalic-salts-sodium-hypochlorite-sodium-sulfide-pretreatment-under-the-autoclave-system
#6
Gang-Gang Chong, Yu-Cai He, Qiu-Xiang Liu, Xiao-Qin Kou, Xiao-Jun Huang, Jun-Hua Di, Cui-Luan Ma
In this study, dilute alkali salts (0.6% NaClO, 0.067% Na2S) pretreatment at 10% sulfidity under the autoclave system at 120°C for 40min was used for pretreating bamboo shoot shell (BSS). Furthermore, FT-IR, XRD and SEM were employed to characterize the changes in the cellulose structural characteristics (porosity, morphology, and crystallinity) of the pretreated BSS solid residue. After 72h, the reducing sugars and glucose from the enzymatic in situ hydrolysis of 50g/L pretreated BSS in dilute NaClO/Na2S media could be obtained at 31...
June 1, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28622484/bactericidal-effect-of-er-yag-laser-activated-sodium-hypochlorite-irrigation-against-biofilms-of-enterococcus-faecalis-isolate-from-canal-of-root-filled-teeth-with-periapical-lesions
#7
Xiaogang Cheng, Doudou Xiang, Wenxi He, Jun Qiu, Bing Han, Qing Yu, Yu Tian
OBJECTIVE: This study was to evaluate the bactericidal effect of Er:YAG laser-activated sodium hypochlorite irrigation (Er:YAG + NaOCl) on biofilms of Enterococcus faecalis clinical isolate. BACKGROUND DATA: It was reported that Er:YAG + NaOCl had effective bactericidal effect on laboratory-adapted E. faecalis strain, while no study has reported its effect on the clinical isolate. METHODS: Eighteen E. faecalis strains were isolated from 39 root-filled teeth with periapical lesions, and their biofilm formation abilities were evaluated using the crystal violet staining method...
June 16, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28620636/a-scanning-electron-microscopic-evaluation-of-the-effectiveness-of-etidronic-acid-smearclear-and-mtad-in-removing-the-intracanal-smear-layer
#8
Hemant Kumar Yadav, Rakesh Kumar Yadav, Anil Chandra, Aseem Prakash Tikku
STATEMENT OF THE PROBLEM: Root canal therapy should not simply be the extirpation of the pulp and widening of the canal. But one should also focus on how to completely remove the loosely-attached smear layer because it has adverse effects on the final outcome of the treatment. PURPOSE: This study compared the efficacy of Etidronic acid, SmearClear and MTAD to remove the smear layer created during instrumentation in different regions of the root canal. MATERIALS AND METHOD: Fifty single-rooted mandibular premolars were decoronated from the cementoenamel junction and instrumented using the ProTaper universal rotary file system along with copious irrigation by 1...
June 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28620631/the-effect-of-titanium-tetrafluoride-and-sodium-hypochlorite-on-the-shear-bond-strength-of-methacrylate-and-silorane-based-composite-resins-an-in-vitro-study
#9
Farahnaz Sharafeddin, Fatemeh Koohpeima, Nader Razazan
STATEMENT OF THE PROBLEM: The bond strength of composites with different adhesive systems with dentin is an important factor in long term durability of composite restorations. The effect of titanium tetrafluoride (TiF4) as anti caries agent and sodium hypochlorite (NaOCl) as disinfectant on the shear bond of nanofilled and silorane based composite resins have not been investigated in previous studies. PURPOSE: This study was conducted to determine bond strength between dentin and two composite systems, by means of shear bond test using TiF4 and NaOCl...
June 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/28620254/isolation-morphological-identification-and-in-vitro-antibacterial-activity-of-endophytic-bacteria-isolated-from-azadirachta-indica-neem-leaves
#10
Ankit Kumar Singh, Rajesh Kumar Sharma, Varsha Sharma, Tanmay Singh, Rajesh Kumar, Dimple Kumari
AIM: The objective of this study was to isolate endophytic bacteria from Azadirachta indica (neem) leaves, their identification and investigate their antibacterial activity against three Gram-positive bacteria, Staphylococcus aureus, Streptococcus pyogenes and Bacillus cereus and Gram-negative bacteria Escherichia coli, Salmonella Typhimurium and Klebsiella pneumoniae. MATERIALS AND METHODS: Fresh leaves of A. indica (neem) was procured from the Department of Botany, JNKVV, Jabalpur...
May 2017: Veterinary World
https://www.readbyqxmd.com/read/28610932/automatic-environmental-disinfection-with-hydrogen-peroxide-and-ion-silver-versus-manual-environmental-disinfection-with-sodium-hypochlorite-a-multicenter-randomized-before-and-after-trial
#11
D Mosci, G W Marmo, L Sciolino, C Zaccaro, R Antonellini, L Accogli, T Lazzarotto, M Mongardi, M P Landini
AIMS: 1. Compare the effectiveness of an automated disinfection system with hydrogen peroxide <8% and silver ion versus a manual method with 0.5% sodium hypochlorite solution to evaluate the reduction of microbial mesophilic contamination and Clostridium difficile (CD) presence; 2. Evaluate the organizational impact of these two technologies in terms of time required for the process. METHODS: A randomized multicenter trial performed in four public and private health facilities in the Emilia-Romagna Region, in different wards including medicine, orthopaedics, long-term care, recovery and functional rehabilitation, which were previously occupied by patients with Clostridium difficile Infection (CDI)...
June 10, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28606669/effectiveness-in-the-removal-of-endotoxins-and-microbiological-profile-in-primary-endodontic-infections-using-3-different-instrumentation-systems-a-randomized-clinical-study
#12
Daiana Cavalli, Cássia Cestari Toia, Esteban Isai Flores Orozco, Rayana Duarte Khoury, Flávia Goulart da Rosa Cardoso, Marcelo Corrêa Alves, Cláudio Antônio Talge Carvalho, Marcia Carneiro Valera
INTRODUCTION: This clinical study was conducted to correlate the microbiological profile and levels of endotoxins found in primary endodontic infection with the presence of clinical features and to evaluate the removal of microorganisms and endotoxins using rotary, reciprocating, and hybrid systems for biomechanical preparation. METHODS: Thirty single root canals with primary endodontic infection were evaluated with signs and symptoms and were randomly divided into 3 groups according to the instrumentation system used (n = 10) as follows: rotary Mtwo instruments (VDW, Munich, Germany) with 8 files, the reciprocating Reciproc system (VDW) with a single file, and Genius hybrid instruments with 3 files (1 rotary and 2 reciprocating files) with irrigation using 24 mL 2...
June 9, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28605289/effect-of-sub-lethal-concentrations-of-biocides-on-the-structural-parameters-and-viability-of-the-biofilms-formed-by-salmonella-typhimurium
#13
Rosa Capita, Laura Buzón-Durán, Félix Riesco-Peláez, Carlos Alonso-Calleja
This study aimed to investigate the effect of sub-minimum inhibitory concentrations (sub-MICs) of three food-grade biocides (benzalkonium chloride -BZK-, trisodium phosphate -TSP-, and sodium hypochlorite -SHY-) on Salmonella biofilms. The structural parameters and bacterial viability of the biofilms formed by a S. Typhimurium isolate from poultry was investigated by means of confocal laser scanning microscopy after staining with SYTO9 and propidium iodide. The MIC values for Salmonella cells before exposure to subinhibitory concentrations of biocides were 8...
June 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28597006/effect-of-final-irrigation-protocol-and-etching-mode-on-bond-strength-of-a-multimode-adhesive-in-the-root-canal
#14
Kerstin Bitter, Luise Polster, Haitham Askar, Manja von Stein-Lausnitz, Guido Sterzenbach
PURPOSE: To analyze the effects of ethanol for final post space irrigation and etching mode on the bond strength of fiber posts luted with a mild multimode adhesive (pH 2.3) as compared with a reference group using a self-adhesive resin cement (SAR). MATERIALS AND METHODS: Human anterior teeth were endodontically treated. After post space preparation, the root canals were irrigated using 1% sodium hypochlorite (NaOCl) applied with passive ultrasonic irrigation, followed by either distilled water (control) or distilled water and ethanol 99% as final post space irrigation...
June 8, 2017: Journal of Adhesive Dentistry
https://www.readbyqxmd.com/read/28593370/identification-of-optimum-fatty-acid-extraction-methods-for-two-different-microalgae-phaeodactylum-tricornutum-and-haematococcus-pluvialis-for-food-and-biodiesel-applications
#15
Paz Otero, Sushanta Kumar Saha, Joanne Mc Gushin, Siobhan Moane, John Barron, Patrick Murray
Microalgae have the potential to synthesize and accumulate lipids which contain high value fatty acids intended for nutrition and biodiesel applications. Nevertheless, lipid extraction methods for microalgae cells are not well established and there is not a standard analytical methodology to extract fatty acids from lipid-producing microalgae. In this paper, current lipid extraction procedures employing organic solvents (chloroform/methanol, 2:1 and 1:2, v/v), sodium hypochlorite solution (NaClO), acid-catalysed hot-water extraction and the saponification process [2...
June 7, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28577962/free-active-chlorine-in-sodium-hypochlorite-solutions-admixed-with-octenidine-smearoff-chlorhexidine-and-edta
#16
Unni Krishnan, Sreeja Saji, Roger Clarkson, Ratilal Lalloo, Alex J Moule
INTRODUCTION: The therapeutic effects of sodium hypochlorite (NaOCl) solutions are dependent on the levels of free available chlorine (FAC). Mixing these solutions with irrigants can result in significant reductions in FAC. Although the effect of some irrigants on FAC is known, the effect of other commonly used irrigants is not. Thus, the therapeutic ramifications of the concurrent use of these on the efficiency of NaOCl solutions is not known. METHODS: Aliquots of 5...
May 31, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28577961/histologic-evaluation-of-regenerative-endodontic-procedures-with-the-use-of-chitosan-scaffolds-in-immature-dog-teeth-with-apical-periodontitis
#17
Paulo J Palma, João C Ramos, João B Martins, Anibal Diogenes, Maria H Figueiredo, Paula Ferreira, Carlos Viegas, João M Santos
INTRODUCTION: The aim of this study was to evaluate histologically the newly formed tissues after regenerative endodontic procedures (REPs) in dogs using either a blood clot (BC) or 2 different formulations of a chitosan hydrogel as scaffolds. METHODS: Apical periodontitis was induced by inoculating immature teeth with oral plaque in 4 beagle dogs. Teeth (n = 96) were divided into 2 control (n = 20) and 4 test groups (n = 76) according to the treatment: apexification and REPs with BC, sodium hyaluronate:chitosan (HA:CS) scaffolds, or pectin:chitosan (P:CS) scaffolds...
May 31, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28577456/dna-decontamination-of-fingerprint-brushes
#18
Bianca Szkuta, Roland A H van Oorschot, Kaye N Ballantyne
Genetic profiling of DNA collected from fingerprints that have been exposed to various enhancement techniques is routine in many forensic laboratories. As a result of direct contact with fingermark residues during treatment, there is concern around the DNA contamination risk of dusting fingermarks with fingerprint brushes. Previous studies have demonstrated the potential for cross-contamination between evidentiary items through various mechanisms, highlighting the risk of using the same fingerprint brush to powder multiple surfaces within and between crime-scenes...
May 22, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28576338/susceptibility-of-ten-haemonchus-contortus-isolates-from-different-geographical-origins-towards-acetone-water-extracts-of-polyphenol-rich-plants-part-2-infective-l3-larvae
#19
J I Chan-Pérez, J F J Torres-Acosta, C A Sandoval-Castro, G S Castañeda-Ramírez, G Vilarem, C Mathieu, H Hoste
This study explored the variation in susceptibility to acetone:water plant extracts between infective larvae (L3) of ten Haemonchus contortus isolates from different geographical origin. The L3 of 10 different isolates were exposed either to the acetone:water extract of a temperate plant (Onobrychis viciifolia) or a tropical plant (Acacia pennatula) and were evaluated with the larval exsheathment inhibition assay (LEIA). The L3 of each isolate were incubated with different concentrations of each extract (0, 25, 50, 100, 200, 400, 600, 800, 1000 and 1200μg/mL of phosphate buffered saline (PBS))...
June 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28571279/comparative-evaluation-of-different-modes-of-laser-assisted-endodontics-in-primary-teeth-an-in-vitro-study
#20
Prabhakar Ramasetty Attiguppe, Krupa Kishore Tewani, Saraswathi V Naik, Chandrashekar M Yavagal, Basappa Nadig
INTRODUCTION: The presence of bacteria in root canals has been considered to be responsible for endodontic treatment failure, even in case of primary teeth. The use of lasers can be a valuable addition in removing bacterial load in areas where traditional methods may fail to succeed. Methods like direct laser irradiation of canals, Laser Activated Irrigation (LAI) with sodium hypochlorite (NaOCl) and Photodynamic Therapy (PDT) seem to be a promising alternative for disinfection. AIM: This study was designed to compare the efficacy of latest advancements in disinfection techniques using diode laser namely direct laser irradiation, photodynamic therapy and laser activated irrigation using sodium hypochlorite...
April 2017: Journal of Clinical and Diagnostic Research: JCDR
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