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https://www.readbyqxmd.com/read/29055204/influence-of-bizerte-city-wastewater-treatment-plant-wwtp-on-abundance-and-antibioresistance-of-culturable-heterotrophic-and-fecal-indicator-bacteria-of-bizerte-lagoon-tunisia
#1
Mariem Souissi, Rached Laabidi, Patricia Aissa, Olivier Pringault, Olfa Ben Said
The waste water treatment plant (WWTP) of the city of Bizerte concentrates different types of chemical and biological pollutants in the Bizerte lagoon (Tunisia). Considering four upstream and downstream WWTP discharge stations, seventy nine, culturable bacterial strains were isolated and identified from water and sediment as fecal coliforms, fecal streptococci, pathogenic staphylococci and non-enterobacteriacea. Fecal coliforms were most abundant (2.5 10(5) bacteria/mg) in sediment of WWTP discharge. Leuconostoc spp (23...
October 18, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29054666/occurrence-and-diversity-of-antibiotic-resistance-in-untreated-hospital-wastewater
#2
Qiang Wang, Panliang Wang, Qingxiang Yang
Antibiotics, antibiotic-resistant bacteria (ARB), antibiotic-resistance genes (ARGs), and mobile genetic elements (MGEs) have been reported in many environments. However, the investigation of their occurrence and diversity in untreated hospital wastewater is still insufficient. High concentrations of antibiotic residues were found in hospital wastewater using solid-phase extraction and UPLC-MS/MS analysis. The concentrations of six of 14 antibiotics reached μg/L levels in the hospital wastewater, which is higher than reported in other aquatic environments...
October 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29054663/characterization-of-bacterial-diversity-in-contaminated-groundwater-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#3
Misty S Martin, Inês C Santos, Doug D Carlton, Paula Stigler-Granados, Zacariah L Hildenbrand, Kevin A Schug
Groundwater is a major source for drinking water in the United States, and therefore, its quality and quantity is of extreme importance. One major concern that has emerged is the possible contamination of groundwater due to the unconventional oil and gas extraction activities. As such, the impacts of exogenous contaminants on microbial ecology is an area to be explored to understand what are the chemical and physical conditions that allow the proliferation of pathogenic bacteria and to find alternatives for water treatment by identifying organic-degrading bacteria...
October 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29054657/feed-additives-shift-gut-microbiota-and-enrich-antibiotic-resistance-in-swine-gut
#4
Yi Zhao, Jian-Qiang Su, Xin-Li An, Fu-Yi Huang, Christopher Rensing, Kristian Koefoed Brandt, Yong-Guan Zhu
Antibiotic resistance genes (ARGs) are emerging environmental contaminants posing a threat to public health. Antibiotics and metals are widely used as feed additives and could consequently affect ARGs in swine gut. In this study, high-throughput quantitative polymerase chain reaction (HT-qPCR) based ARG chip and next-generation 16S rRNA gene amplicon sequencing data were analyzed using multiple statistical approaches to profile the antibiotic resistome and investigate its linkages to antibiotics and metals used as feed additives and to the microbial community composition in freshly collected swine manure samples from three large-scale Chinese pig farms...
October 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29054656/development-and-evaluation-of-diffusive-gradients-in-thin-films-technique-for-measuring-antibiotics-in-seawater
#5
Huaijun Xie, Jingwen Chen, Qining Chen, Chang-Er L Chen, Juan Du, Feng Tan, Chengzhi Zhou
As coastal waters in many regions of the world have been polluted by organic micro-pollutants such as antibiotics that can promote the development of resistance genes, it is of importance to monitor the levels of antibiotics in coastal waters. For this purpose, a reliable and robust sampling approach based on diffusive gradients in thin films (DGT) was developed in this study. The DGT measurement can provide a time weighted average concentration of pollutants. A binding material (resin XDA-1) with a high adsorption capacity for antibiotics, which can resist the pH and ionic strength of seawaters, was selected...
October 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29054550/-mlva-analysis-and-virulence-profile-of-community-acquired-methicillin-resistant-staphylococcus-aureus-causing-infections-in-paraguayan-children
#6
Fátima Rodríguez Acosta, Wilma D Basualdo Acuña, Héctor Castro, Ana Campuzano, María L Macchi, Juana Ortellado de Canese, Patricia Almada, Mónica Rodríguez, Lorena Grau, Gladys Velázquez, Carmen Espínola, Gloria C Samudio Domínguez, Gloria Gómez, Letizia Carpinelli, Rosa M Guillén Fretes
Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is one of the first causes of skin and soft tissue infections, and can also produce severe diseases such as osteomyelitis and pneumonia. The aim of this descriptive study was to determine the SCCmec type and virulence profile and to study the genetic diversity by MLVA analysis of 21 CA-MRSA isolates that infected Paraguayan children in 2010. The SCCmec type and virulence factors were performed by PCR and genetic diversity by MLVA (multiple locus variable analysis)...
October 17, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29054298/antimicrobial-resistance-in-community-acquired-urinary-tract-infections-in-paris-in-2015
#7
D Chervet, O Lortholary, J-R Zahar, A Dufougeray, B Pilmis, H Partouche
BACKGROUND: Urinary tract infection (UTI) is one of the most frequent community-acquired infection. Escherichia coli resistance has been on the rise since 2000s. METHODS: We conducted a prospective multicenter cohort study including adults who had a positive urine cytobacteriological examination (UCBE) performed in our Parisian suburb laboratory platform from October 2014 to March 2015. RESULTS: A total of 1223 patients were included: 995 (81...
October 17, 2017: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/29054003/adhesion-force-sensing-and-activation-of-a-membrane-bound-sensor-to-activate-nisin-efflux-pumps-in-staphylococcus-aureus-under-mechanical-and-chemical-stresses
#8
Vera Carniello, Akshay K Harapanahalli, Henk J Busscher, Henny C van der Mei
Nisin-associated-sensitivity-response-regulator (NsaRS) in Staphylococcus aureus is important for its adhesion to surfaces and resistance against antibiotics, like nisin. NsaRS consists of an intra-membrane-located sensor NsaS and a cytoplasmatically-located response-regulator NsaR, which becomes activated upon receiving phosphate groups from the intra-membrane-located sensor. HYPOTHESIS: The intra-membrane location of the NsaS sensor leads us to hypothesize that the two-component NsaRS system not only senses "chemical" (nisin) but also "mechanical" (adhesion) stresses to modulate efflux of antibiotics from the cytoplasm...
October 7, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29053557/bacterial-keratitis-isolated-organisms-and-antibiotic-resistance-patterns-in-san-francisco
#9
Michelle Y Peng, Vicky Cevallos, Stephen D McLeod, Thomas M Lietman, Jennifer Rose-Nussbaumer
PURPOSE: Understanding the spectrum of pathogens in a given geographic region is important when deciding on empiric antibiotic therapy. In this study, we evaluate the spectrum of bacterial organisms cultured from corneal samples and their antibiotic sensitivities to guide initial treatment of keratitis. METHODS: We performed a retrospective case review of cultures from suspected infectious keratitis cases at the Francis I. Proctor Foundation, University of California, San Francisco, from 1996 through 2015...
October 17, 2017: Cornea
https://www.readbyqxmd.com/read/29052797/suppressive-antibiotic-therapy-with-oral-tetracyclines-for-prosthetic-joint-infections-a-retrospective-study-of-78-patients
#10
M Pradier, O Robineau, A Boucher, M Titecat, N Blondiaux, M Valette, C Loïez, E Beltrand, S Nguyen, H Dézeque, H Migaud, Eric Senneville
PURPOSE: This study aimed at describing the use of oral cyclines (i.e., doxycycline and minocycline) as suppressive antibiotic therapy (SAT) in patients with periprosthetic joint infections (PJIs). METHODS: Medical charts of all patients with surgical revisions for PJIs who were given cycline-based SAT because of a high failure of various origins were reviewed. Data regarding tolerability and effectiveness of cycline-based SAT were analysed. RESULTS: Seventy-eight patients of mean age 64 ± 17 years received cycline-base SAT in the period from January 2006 to January 2014...
October 20, 2017: Infection
https://www.readbyqxmd.com/read/29052326/effects-of-the-antimicrobial-peptide-protegrine-1-on-sperm-viability-and-bacterial-load-of-boar-seminal-doses
#11
S Sancho, M Briz, M Yeste, S Bonet, E Bussalleu
The presence of bacteria adversely affects boar sperm quality of seminal doses intended for artificial insemination. Currently, the most common measure to prevent bacteriospermia is the addition of antibiotics in semen extenders; however, mounting evidence shows that microbial resistance exists. A promising alternative to replace antibiotics are antimicrobial peptides. In this study, the effects of the antimicrobial peptide protegrine 1 (PG1) on the sperm viability and bacterial load of boar seminal doses were evaluated...
October 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29052092/a-72-h-intervention-for-improvement-of-the-rate-of-optimal-antibiotic-therapy-in-patients-with-bloodstream-infections
#12
R Murri, F Taccari, T Spanu, T D'Inzeo, I Mastrorosa, F Giovannenze, G Scoppettuolo, G Ventura, C Palazzolo, M Camici, S Lardo, B Fiori, M Sanguinetti, R Cauda, M Fantoni
Antimicrobial stewardship programs are implemented to optimize the use of antibiotics and control the spread of antibiotic resistance. Many antimicrobial stewardship interventions have demonstrated significant efficacy in reducing unnecessary prescriptions of antibiotics, the duration of antimicrobial therapy, and mortality. We evaluated the benefits of a combination of rapid diagnostic tests and an active re-evaluation of antibiotic therapy 72 h after the onset of bloodstream infection (BSI). All patients with BSI from November 2015 to November 2016 in a 1100-bed university hospital in Rome, where an Infectious Disease Consultancy Unit (Unità di Consulenza Infettivologica, UDCI) is available, were re-evaluated at the bedside 72 h after starting antimicrobial therapy and compared to two pre-intervention periods: the UDCI was called by the ward physician for patients with BSI and the UDCI was called directly by the microbiologist immediately after a pathogen was isolated from blood cultures...
October 19, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29051752/icesag37-a-novel-integrative-and-conjugative-element-carrying-antimicrobial-resistance-genes-and-potential-virulence-factors-in-streptococcus-agalactiae
#13
Kaixin Zhou, Lianyan Xie, Lizhong Han, Xiaokui Guo, Yong Wang, Jingyong Sun
ICESag37, a novel integrative and conjugative element carrying multidrug resistance and potential virulence factors, was characterized in a clinical isolate of Streptococcus agalactiae. Two clinical strains of S. agalactiae, Sag37 and Sag158, were isolated from blood samples of new-borns with bacteremia. Sag37 was highly resistant to erythromycin and tetracycline, and susceptible to levofloxacin and penicillin, while Sag158 was resistant to tetracycline and levofloxacin, and susceptible to erythromycin. Transfer experiments were performed and selection was carried out with suitable antibiotic concentrations...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29051747/pirab-vp-bearing-vibrio-parahaemolyticus-and-vibrio-campbellii-pathogens-isolated-from-the-same-ahpnd-affected-pond-possess-highly-similar-pathogenic-plasmids
#14
Xuan Dong, Dexi Bi, Hailiang Wang, Peizhuo Zou, Guosi Xie, Xiaoyuan Wan, Qian Yang, Yanping Zhu, Mengmeng Chen, Chengcheng Guo, Zhen Liu, Wenchao Wang, Jie Huang
Acute hepatopancreatic necrosis disease (AHPND) is a severe shrimp disease originally shown to be caused by virulent strains of Vibrio parahaemolyticus (VPAHPND). Rare cases of AHPND caused by Vibrio species other than V. parahaemolyticus were reported. We compared an AHPND-causing V. campbellii (VCAHPND) and a VPAHPND isolate from the same AHPND-affected pond. Both strains are positive for the virulence genes pirAB(vp) . Immersion challenge test with Litopenaeus vannamei indicated the two strains possessed similar pathogenicity...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29051656/antimicrobial-resistance-in-diarrheagenic-escherichia-coli-from-ready-to-eat-foods
#15
Cíntia Matos Lima, Ingrid Evelyn Gomes Lima Souza, Taila Dos Santos Alves, Clícia Capibaribe Leite, Norma Suely Evangelista-Barreto, Rogeria Comastri de Castro Almeida
Certain subgroups of Escherichia coli have congenital or acquired virulence properties that allow them to cause a wide spectrum of disease. The aim of this study was to investigate the occurrence of diarrheagenic E. coli strains in ready-to-eat (RTE) foods produced in institutional, commercial and hotel restaurants in Salvador, Brazil. The presence of virulent isolates and antimicrobial resistance were evaluated. Four hundred forty-six samples were collected and grouped into cereals and vegetables, meat-based preparations, cooked salads, raw salads, garnishes, soups and sauces, desserts and juices...
October 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29051620/antimicrobial-peptide-loaded-gold-nanoparticle-dna-aptamer-conjugates-as-highly-effective-antibacterial-therapeutics-against-vibrio-vulnificus
#16
Boeun Lee, Jonggwan Park, Minkyung Ryu, Soochan Kim, Minju Joo, Ji-Hyun Yeom, Suk Kim, Yoonkyung Park, Kangseok Lee, Jeehyeon Bae
Vibrio vulnificus causes fatal infections in humans, and antibiotics are commonly used in treatment regimens against V. vulnificus infection. However, the therapeutic effects of antibiotics are limited by multidrug resistance. In this study, we demonstrated that an antimicrobial peptide (AMP), HPA3P(His), loaded onto a gold nanoparticle-DNA aptamer (AuNP-Apt) conjugate (AuNP-Apt-HPA3P(His)) is an effective therapeutic tool against V. vulnificus infection in vivo in mice. HPA3P(His) induced bacterial cell death through the disruption of membrane integrity of V...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29051379/ectopic-colonization-of-oral-bacteria-in-the-intestine-drives-th1-cell-induction-and-inflammation
#17
Koji Atarashi, Wataru Suda, Chengwei Luo, Takaaki Kawaguchi, Iori Motoo, Seiko Narushima, Yuya Kiguchi, Keiko Yasuma, Eiichiro Watanabe, Takeshi Tanoue, Christoph A Thaiss, Mayuko Sato, Kiminori Toyooka, Heba S Said, Hirokazu Yamagami, Scott A Rice, Dirk Gevers, Ryan C Johnson, Julia A Segre, Kong Chen, Jay K Kolls, Eran Elinav, Hidetoshi Morita, Ramnik J Xavier, Masahira Hattori, Kenya Honda
Intestinal colonization by bacteria of oral origin has been correlated with several negative health outcomes, including inflammatory bowel disease. However, a causal role of oral bacteria ectopically colonizing the intestine remains unclear. Using gnotobiotic techniques, we show that strains of Klebsiella spp. isolated from the salivary microbiota are strong inducers of T helper 1 (TH1) cells when they colonize in the gut. These Klebsiella strains are resistant to multiple antibiotics, tend to colonize when the intestinal microbiota is dysbiotic, and elicit a severe gut inflammation in the context of a genetically susceptible host...
October 20, 2017: Science
https://www.readbyqxmd.com/read/29051236/first-complete-genome-sequence-of-methicillin-resistant-staphylococcus-aureus-strain-so-1977-isolated-from-khartoum-sudan
#18
Sofia B Mohamed, Mohamed S Ali, Faisal M Alamir, Tahani B Alyas, Abdallah E Ahmed, Almeen O Seed, Rihab A Omer
Methicillin-resistant Staphylococcus aureus is increasingly becoming resistant to most antibiotics and consequently has become a challenging public health problem in Sudan. The present study documented the first complete genome sequence of strain SO-1977, isolated from a contaminated wound in Sudan.
October 19, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29051234/draft-genome-sequence-of-a-salmonella-enterica-serovar-typhi-strain-resistant-to-fourth-generation-cephalosporin-and-fluoroquinolone-antibiotics
#19
Danish Gul, Robert F Potter, Hurmat Riaz, Shifa Tariq Ashraf, Meghan A Wallace, Tehmina Munir, Amjad Ali, C A Burnham, Gautam Dantas, Saadia Andleeb
Typhoid is endemic in developing countries. We report here the first draft genome sequence of a Salmonella enterica serovar Typhi clinical isolate from Pakistan exhibiting resistance to cefepime (a fourth-generation cephalosporin) and fluoroquinolone antibiotics, two of the last-generation therapies against this pathogen. The genome is ~4.8 Mb, with two putative plasmids.
October 19, 2017: Genome Announcements
https://www.readbyqxmd.com/read/29051020/draft-genome-sequences-of-three-multidrug-resistant-cutibacterium-formerly-propionibacterium-acnes-strains-isolated-from-acne-patients-china
#20
Nanxue Zhang, Zhong Lu, Ying Ma
OBJECTIVES: Cutibacterium acnes (formerly Propionibacterium acnes), an anaerobic Gram-positive bacterium, is a skin commensal and considered to be associated with acne. Here we report draft genome sequences of three multidrug-resistant C. acnes strains (CA17, CA39, and CA51), isolated from skin lesions of acne patients from China. METHODS: The whole genome of the three multidrug-resistant C. acnes strains were sequenced using an Illumina HiSeq 2500 platform. De novo assembly and annotation were performed with SPAdes-3...
October 16, 2017: Journal of Global Antimicrobial Resistance
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