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Katherine J Harrison, Valérie de Crécy-Lagard, Rémi Zallot
Summary: The examination of gene neighborhood is an integral part of comparative genomics but no tools to produce publication quality graphics of gene clusters are available. Gene Graphics is a straightforward web application for creating such visuals. Supported inputs include National Center for Biotechnology Information (NCBI) gene and protein identifiers with automatic fetching of neighboring information, GenBank files and data extracted from the SEED database. Gene representations can be customized for many parameters including gene and genome names, colors and sizes...
December 7, 2017: Bioinformatics
Xiaomeng Han, Zhen Zhou, Xiaojie Mei, Yan Ma, Zhenfang Xie
In order to investigate effects of waste activated sludge (WAS) fermentation liquid on anoxic/oxic- membrane bioreactor (A/O-MBR), two A/O-MBRs with and without WAS fermentation liquid addition were operated in parallel. Results show that addition of WAS fermentation liquid clearly improved denitrification efficiency without deterioration of nitrification, while severe membrane fouling occurred. WAS fermentation liquid resulted in an elevated production of proteins and humic acids in bound extracellular polymeric substance (EPS) and release of organic matter with high MW fractions in soluble microbial product (SMP) and loosely bound EPS (LB-EPS)...
December 2, 2017: Bioresource Technology
Rinkesh Kumar Bansal, Gaurav Kumar Patil, Rajesh Puri, Narendra S Choudhary, Saurabh R Patle, Zubin D Sharma, Randhir Sud
Background and aim:  There is paucity of data about endoscopic pancreatic sphincteroplasty (EPS) after endoscopic pancreatic sphincterotomy (EPST) in the treatment of chronic pancreatitis. The aim of this study was to establish the indications for EPS, complications related to it, and to examine its effectiveness in managing chronic pancreatitis after a year of follow-up. Methods:  We evaluated the safety and efficacy of pancreatic balloon dilation coupled with sphincterotomy for the treatment of chronic pancreatitis...
December 2017: Endoscopy International Open
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No abstract text is available yet for this article.
September 2017: International Journal of Pharmaceutical Compounding
Yijing Zhu, Yayi Wang, Shuai Zhou, Xuxin Jiang, Xiao Ma, Chao Liu
Antibiotic resistance genes (ARGs) are an emerging concern in wastewater treatment plants (WWTPs), as dissemination of ARGs can pose a serious risk to human health. Few studies, however, have quantified ARGs in membrane bioreactors (MBRs), although MBRs have been widely used for both municipal and industrial wastewater treatment. To reveal the capacity of MBRs for removal of ARGs and the response of membrane fouling after antibiotic exposure, five typical ARG subtypes (sulI, sulII, tetC, tetX and ereA) and int1 were quantified affiliated by systematic membrane foulants analysis in a laboratory-scale anoxic/aerobic membrane bioreactor (A/O-MBR)...
November 30, 2017: Water Research
Jun Chen, Cuiqin Yin, Jie Jin, Ke Wu, Zhimin Yu, Chengxun Deng
Ultraviolet-visible (UV-Vis) absorbance spectra were adopted to quantify the binding of metal ions (e.g., Fe(III), Cu(II), Pb(II), and Cd(II)) on three MW fractions (> 100, 10~100, and < 10 k Da) of extracellular polymeric substances (EPS) extracted from mixed cultures dominated by anaerobic ammonium-oxidizing bacteria (AnAOB). The results showed that the AnAOB EPS with different MW size ranges all had strongest binding capability of Fe(III), and the lowest binding capability of Cd(II). The complexation ability of metal ions for the EPS of AnAOB with molecular weight < 10 kDa was stronger than EPS with >100 and 10~100 kDa, very likely because of the contribution of the tyrosine-, tryptophan-, and aromatic protein-like components...
December 7, 2017: Environmental Science and Pollution Research International
Fany M Iseka, Benjamin T Goetz, Insha Mushtaq, Wei An, Luke R Cypher, Timothy A Bielecki, Eric C Tom, Priyanka Arya, Sohinee Bhattacharyya, Matthew D Storck, Craig L Semerad, James E Talmadge, R Lee Mosley, Vimla Band, Hamid Band
T cells use the endocytic pathway for key cell biological functions, including receptor turnover and maintenance of the immunological synapse. Some of the established players include the Rab GTPases, the SNARE complex proteins, and others, which function together with EPS-15 homology domain-containing (EHD) proteins in non-T cell systems. To date, the role of the EHD protein family in T cell function remains unexplored. We generated conditional EHD1/3/4 knockout mice using CD4-Cre and crossed these with mice bearing a myelin oligodendrocyte glycoprotein-specific TCR transgene...
December 6, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
Zhan-Peng Sun, An-Qi Li, Wen-Huan Jia, Sen Ye, Grace Van Eps, Jian-Min Yu, Wei-Jun Yang
Cancer stem-like cells (CSCs) have been identified as the initial cell in formation of cancer. Quiescent CSCs can "hide out" from traditional cancer therapy which may produce an initial response but are often unsuccessful in curing patients. Thus, levels of CSC in patients may be used as an indicator to measure the chance of recurrence of cancer after therapy. The goals of our work are to develop specific exosomal miRNA clusters for gastric CSCs that can potentially predict which patients are at high risk for developing gastric cancer (GC) in order to diagnose GC at an early stage...
November 7, 2017: Oncotarget
Teresa Chan, Mathew Mercuri, Kenneth Van Dewark, Jonathan Sherbino, Alan Schwartz, Geoff Norman, Matthew Lineberry
PURPOSE: Emergency physicians (EPs) regularly manage multiple patients simultaneously, often making time-sensitive decisions around priorities for multiple patients. Few studies have explored physician cognition in multi-patient scenarios. The authors sought to develop a conceptual framework to describe how EPs think in busy, multi-patient environments. METHOD: From July 2014 to May 2015, a qualitative study was conducted at McMaster University, using a think aloud protocol to examine how 10 attending EPs and 10 junior residents made decisions in multi-patient environments...
November 28, 2017: Academic Medicine: Journal of the Association of American Medical Colleges
Xiaochun Huang, Chang Chen, Chunhua Ren, Yingying Li, Yiqin Deng, Yiying Yang, Xiongqi Ding
Colanic acid (CA) is a group I extracellular polysaccharide (EPS) that contributes to resistance against adverse environments in many members of the Enterobacteriaceae. In the present study, a genetic locus EPSC putatively involved in CA biosynthesis was identified in Vibrio alginolyticus ZJ-51, which undergoes colony morphology variation between translucent/smooth (ZJ-T) and opaque/rugose (ZJ-O). EPSC in ZJ-T carries 21 ORFs and resembles the CA cluster of Escherichia coli K-12. The deletion of EPSC led to decreased EPS and biofilm formation in both genetic backgrounds but no alternation of lipopolysaccharide...
December 6, 2017: Biofouling
Woojae Myung, Jin Hong Park, Sook-Young Woo, Seonwoo Kim, Sang Ha Kim, Jae Won Chung, Hyo Shin Kang, Shinn-Won Lim, Junbae Choi, Duk L Na, Seong Yoon Kim, Jae-Hong Lee, Seol-Heui Han, Seong Hye Choi, Sang Yun Kim, Bernard J Carroll, Doh Kwan Kim
Objective: Extrapyramidal signs (EPS) are common in patients with mild cognitive impairment (MCI). However, few studies have assessed the effect of EPS on the clinical course of MCI. We aimed to evaluate whether patients with EPS show more frequent progression from MCI to Alzheimer's disease (AD) and to other types of dementia. Methods: Participants (n=882) with MCI were recruited, and were followed for up to 5 years. The EPS positive group was defined by the presence of at least one EPS based on a focused neurologic examination at baseline...
November 2017: Psychiatry Investigation
Bruna Corradetti, Francesca Taraballi, Claudia Corbo, Fernando Cabrera, Laura Pandolfi, Silvia Minardi, Xin Wang, Jeffrey Van Eps, Guillermo Bauza, Bradley Weiner, Ennio Tasciotti
In mammals, tissue regeneration is accomplished through a well-regulated, complex cascade of events. The disruption of the cellular and molecular processes involved in tissue healing might lead to scar formation. Most tissue engineering approaches have tried to improve the regenerative outcome following an injury, through the combination of biocompatible materials, stem cells and bioactive factors. However, implanted materials can cause further healing impairments due to the persistent inflammatory stimuli that trigger the onset of chronic inflammation...
December 5, 2017: Scientific Reports
Mahalingam Anjugam, Baskaralingam Vaseeharan, Arokiadhas Iswarya, Mani Divya, Narayanan Marimuthu Prabhu, Krishnasamy Sankaranarayanan
The present study reports the biological synthesis of silver nanoparticles using crustacean immune molecule β-1, 3 glucan binding protein (β-GBP) purified from the haemolymph of blue swimmer crab Portunus pelagicus. The characterization of synthesized β-GBP based silver nanoparticles (Ppβ-GBP-AgNPs) was made by UV-Vis spectroscopy, XRD, FTIR and TEM analysis. UV-Vis spectra recorded the strong absorbance peak at 420 nm due to its surface plasmon resonance. The XRD analysis revealed the crystalline nature of synthesized nanoparticles with Bragg's reflection peaks at (111), (200), (220), (311) planes...
December 2, 2017: Microbial Pathogenesis
Mary Jacob, Hamid Shokoohi, Fabith Moideen, Amelia Pousson, Keith Boniface
Introduction: Focused training in transthoracic echocardiography enables emergency physicians (EPs) to accurately estimate the left ventricular function. This study aimed to evaluate the efficacy of a brief training program utilizing standardized echocardiography video clips in this regard. Methods: A before and after design was used to determine the efficacy of a 1 hour echocardiography training program using PowerPoint presentation and standardized echocardiography video clips illustrating normal and abnormal left ventricular ejection fraction (LVEF) as well as video clips emphasizing the measurement of mitral valve E-point septal separation (EPSS)...
2017: Emergency (Tehran, Iran)
Matgorzata Statkiewicz, Anna Matuszewska, Magdalena Jaszek, Grzegorz Janusz, Monika Osinska, Justyna Sulej, Dawid Stefaniuk, Michat Mikula, Jerzy Ostrowski
Three bioactive fractions isolated from Cerrena unicolor cultures-crude endopolysaccharide (c-EPS), laccase, and a subfraction of low-molecular weight secondary metabolites-were used to determine potential cytotoxic effects on the mouse melanoma B16-F10 cell line (American Type Culture Collection CRL-6475). The results obtained prove that all examined fractions exhibited activity against the investigated tumor cells. In addition, an evident immunomodulatory effect of the c-EPS fraction was observed. Our results show that the levels of 2 cytokines (tumor necrosis factor-a and chemokine ligand 2) in mouse inner medullary collecting duct mIMCD-3 cells (American Type Culture Collection CRL-2123) stimulated by c-EPS were significantly higher...
2017: International Journal of Medicinal Mushrooms
Santosh Pandit, Ji-Eun Jung, Hyeon-Mi Choi, Jae-Gyu Jeon
The present study investigated the effect of periodic 1-min fluoride treatments on Streptococcus mutans biofilms and then determined the relationship between anti-biofilm activity, treatment frequency, and fluoride concentration using a linear-fitting procedure. S. mutans biofilms were periodically treated (1-min/treatment) with fluoride during biofilm formation and analyzed using microbiological methods, confocal microscopy, and real-time PCR. The results indicated that reductions in the dry weight and acidogenicity of biofilms due to periodic fluoride treatment occurred in a concentration dependent manner...
December 4, 2017: Biofouling
Li Ou, Li Ang, Zhang Chujun, Huang Jingyu, Meng Yongli, Yuan Shenjing, Huang Junhua, Gao Xu, Yao Yulong, Yin Rui, Hu Jinpan, Ding Bin, Hu Xiufang
Paenibacillus elgii B69 produces a new xylose-containing exopolysaccharide (EPS) that effectively removes the pollutants from wastewater through flocculation. However, information about the biosynthesis of this EPS is limited. In this study, sequence analysis showed six putative glycosyltransferases (GTs) genes in polysaccharide gene clusters involved in glycosidic linkages of repeating units. Each gene was deleted and phenotypes were examined to understand the functions of these genes. Two of the genes were deleted successfully to encode a priming glucose GT and a side-chain xylose GT, but other genes were unsuccessfully deleted because of the accumulation of toxic intermediate products...
December 3, 2017: Applied Microbiology and Biotechnology
Xiaoping Wan, Mingming Gao, Maosheng Ye, Yun-Kun Wang, Hai Xu, Mingyu Wang, Xin-Hua Wang
The growing occurrence of antibiotics in water environment is causing increasing concern. To investigate the impact of frequently detected sulfadiazine on the formation of aerobic granular sludge, four sequencing batch reactors (SBRs) were set up with different environmentally relevant concentrations of sulfadiazine. Results showed that sulfadiazine pressure could lead to larger and more compact sludge particles and cause slight effect on reactor performance. Presence of sulfadiazine apparently increased the extracellular polymeric substances (EPS) secretion of microorganisms...
November 23, 2017: Bioresource Technology
Consolazione Caruso, Carmen Rizzo, Santina Mangano, Annarita Poli, Paola Di Donato, Barbara Nicolaus, Gaetano Di Marco, Luigi Michaud, Angelina Lo Giudice
The EPS-producing Pseudoalteromonas sp. MER144 was selected among 606 isolates from Antarctic seawater due to its evident slimy appearance on agar plates. The production of EPSs was enhanced by a step-by-step approach varying the carbon source, substrate and NaCl concentrations, temperature, and pH. Optimal conditions for the EPS production resulted at temperature of 4 °C and pH 7, with addition of 2% sucrose (w/v) and 3% NaCl (w/v). EPSs produced under optimal conditions were chemically characterized, resulting in a moderate carbohydrate content (35%), uronic acids (14%), and proteins (12%)...
December 1, 2017: Environmental Science and Pollution Research International
Małgorzata Marczak, Andrzej Mazur, Piotr Koper, Kamil Żebracki, Anna Skorupska
Rhizobia dwell and multiply in the soil and represent a unique group of bacteria able to enter into a symbiotic interaction with plants from the Fabaceae family and fix atmospheric nitrogen inside de novo created plant organs, called nodules. One of the key determinants of the successful interaction between these bacteria and plants are exopolysaccharides, which represent species-specific homo- and heteropolymers of different carbohydrate units frequently decorated by non-carbohydrate substituents. Exopolysaccharides are typically built from repeat units assembled by the Wzx/Wzy-dependent pathway, where individual subunits are synthesized in conjunction with the lipid anchor undecaprenylphosphate (und-PP), due to the activity of glycosyltransferases...
December 1, 2017: Genes
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