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https://www.readbyqxmd.com/read/29456814/ascorbic-acid-augments-colony-spreading-by-reducing-biofilm-formation-of-methicillin-resistant-staphylococcus-aureus
#1
Zulfiqar Ali Mirani, Muhammad Naseem Khan, Anila Siddiqui, Fouzia Khan, Mubashir Aziz, Shagufta Naz, Ayaz Ahmed, Seema Ismat Khan
Objectives: Staphylococcus aureus is a Gram-positive pathogen, well known for its resistance and versatile lifestyle. Under unfavourable conditions, it adapts biofilm mode of growth. For staphylococcal biofilm formation, production of extracellular polymeric substances (EPS) is a pre-requisite, which is regulated by ica operon-encoded enzymes. This study was designed to know the impact of ascorbic acid on biofilm formation and colony spreading processes of S. aureus and MRSA. Materials and Methods: The isolates of methicillin-resistant S...
February 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29455259/influence-of-zn-ii-stress-induction-on-component-variation-and-sorption-performance-of-extracellular-polymeric-substances-eps-from-bacillus-vallismortis
#2
Peifei Ding, Weifeng Song, Ziheng Yang, Jingyi Jian
Bacillus vallismortis (B. vallismortis), an aerobic heterotrophic bacteria, was screened in a laboratory pilot study, to assess the interaction between the heavy metal Zn(II) and extracellular polymeric substances (EPS). The influence of Zn(II) stress on EPS production, component variation, and sorption performance, was investigated. The characteristics of B. vallismortis EPS formed under stress were analyzed using FTIR, 3D-EEM and XPS. EPS was used as an adsorbent and the adsorption capacity and adsorption behavior of EPS formed with and without Zn(II) stress, were compared and assessed...
February 17, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29454277/nitrogen-removal-enhancement-using-lactic-acid-fermentation-products-from-food-waste-as-external-carbon-sources-performance-and-microbial-communities
#3
Jialing Tang, Xiaochang C Wang, Yisong Hu, Yunhui Pu, Jin Huang, Huu Hao Ngo, Yonggang Zeng, Yuyou Li
In this study, nitrogen removal using the lactic acid fermentation products from food waste and other external chemical carbon sources (sodium acetate, sodium lactate and starch) was investigated. Similar to sodium acetate and lactate, the lactic acid-enriched fermentation liquid from food waste (FLFW) exhibited a high denitrification rate (5.5 mg NO x -N/(g-VSS h)) and potential (0.16 g NO 3 - -N/g COD), and could achieve high NH 4 + -N and total nitrogen (TN) removal efficiencies during long-term operation...
February 8, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29454055/optimization-chain-conformation-and-characterization-of-exopolysaccharide-isolated-from-leuconostoc-mesenteroides-drp105
#4
Hanwen Xing, Renpeng Du, Fangkun Zhao, Ye Han, Huazhi Xiao, Zhijiang Zhou
Response surface methodology (RSM) was used to optimize the fermentation condition of exopolysaccharide (EPS) producing strain Leuconostoc mesenteroides DRP105. Result showed that the optimum condition was sucrose 86.83 g/L, tryptone 15.47 g/L, initial pH 7.18 and maximum yield was 53.79 ± 0.78 g/L in 36 h fermentation. Chain conformation was characterized by Congo red test, β-elimination and circular dichroism (CD), which indicated that the EPS was O-linkage and exhibited random coil structure in aqueous solution...
February 14, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29449173/exopolysaccharide-production-from-bacillus-velezensis-ky471306-using-statistical-experimental-design
#5
Saad A M Moghannem, Mohamed M S Farag, Amr M Shehab, Mohamed S Azab
Exopolysaccharide (EPS) biopolymers produced by microorganisms play a crucial role in the environment such as health and bio-nanotechnology sectors, gelling agents in food and cosmetic industries in addition to bio-flocculants in the environmental sector as they are degradable, nontoxic. This study focuses on the improvement of EPS production through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett-Burman design indicated that; molasses, yeast extract and incubation temperature are the most effective parameters...
January 18, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29448346/exceptional-points-near-first-and-second-order-quantum-phase-transitions
#6
Pavel Stránský, Martin Dvořák, Pavel Cejnar
We study the impact of quantum phase transitions (QPTs) on the distribution of exceptional points (EPs) of the Hamiltonian in the complex-extended parameter domain. Analyzing first- and second-order QPTs in the Lipkin-Meshkov-Glick model we find an exponentially and polynomially close approach of EPs to the respective critical point with increasing size of the system. If the critical Hamiltonian is subject to random perturbations of various kinds, the averaged distribution of EPs close to the critical point still carries decisive information on the QPT type...
January 2018: Physical Review. E
https://www.readbyqxmd.com/read/29446045/effects-of-extracellular-orotic-acid-on-acute-contraction-induced-adaptation-patterns-in-c2c12-cells
#7
Thomas Beiter, Jens Hudemann, Christof Burgstahler, Andreas M Nieß, Barbara Munz
Dietary administration of orotic acid (OA), an intermediate in the pyrimidine biosynthetic pathway, is considered to provide a wide range of beneficial effects, including cardioprotection and exercise adaptation. Its mechanisms of action, when applied extracellularly, however, are barely understood. In this study, we evaluated potential effects of OA on skeletal muscle using an in vitro contraction model of electrically pulse-stimulated (EPS) C2C12 myotubes. By analyzing a subset of genes representing inflammatory, metabolic, and structural adaptation pathways, we could show that OA supplementation diminishes the EPS-provoked expression of inflammatory transcripts (interleukin 6, Il6; chemokine (C-X-C Motif) ligand 5, Cxcl5), and attenuated transcript levels of nuclear receptor subfamily 4 group A member 3 (Nr4A3), early growth response 1 (Egr1), activating transcription factor 3 (Atf3), and fast-oxidative MyHC-IIA isoform (Myh2)...
February 14, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29442729/genomic-and-proteomic-evidences-for-exopolysaccharide-biosynthesis-in-sphingobium-chungbukense-dj77
#8
Soo Youn Lee, Simranjeet Singh Sekhon, Mihye Kim, Ji-Young Ahn, Hyung Cheol Kim, Young-Chang Kim, Yang-Hoon Kim
Sphingobium chungbukense DJ77 is a Gram-negative bacterium has metabolic capability of producing exopolysaccharide (EPS) as a potential reducing and stabilizing agent for metallic nanoparticle synthesis. In this study, we investigated the genomic and proteomic analysis to verify metabolic pathway and involved genes and enzymes related to EPS biosynthesis in S. chungbukense DJ77. End-sequencing results of randomly selected fosmid library, which were prepared from high molecular weight DNA of S. chungbukense DJ77, showed identity to sequences from genes related the EPS biosynthesis pathways in several bacteria...
June 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29442149/influence-of-different-sugars-and-initial-ph-on-%C3%AE-glucan-formation-by-lactobacillus-brevis-tmw-1-2112
#9
Marion E Fraunhofer, Frank Jakob, Rudi F Vogel
We wanted to identify key factors influencing the extent of β-glucan production by Lactobacillus brevis TMW 1.2112, which has been isolated from viscous, spoiled beer and which could contribute to viscosity increases of spoiled beverages via exopolysaccharide (EPS) production. In this way, we analyzed the influence of different initial pH values and carbohydrate sources on growth of and slime/β-glucan formation by this strain. In a screening of 48 carbohydrates, 14 fermentable sugars which enabled growth were identified...
February 13, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29441840/the-ethics-of-real-time-ems-direction-suggested-curricular-content
#10
Jay M Brenner, Andrew L Aswegan, Laura E Vearrier, Jesse B Basford, Kenneth V Iserson
Ethical dilemmas can create moral distress in even the most experienced emergency physicians (EPs). Following reasonable and justified approaches can help alleviate such distress. The purpose of this article is to guide EPs providing Emergency Medical Services (EMS) direction to navigate through common ethical issues confronted in the prehospital delivery of care, including protecting privacy and confidentiality, decision-making capacity and refusal of treatment, withholding of treatment, and termination of resuscitation (TOR)...
February 14, 2018: Prehospital and Disaster Medicine
https://www.readbyqxmd.com/read/29439486/biosorption-of-cadmium-by-non-toxic-extracellular-polymeric-substances-eps-synthesized-by-bacteria-from-marine-intertidal-biofilms
#11
Juan Carlos Camacho-Chab, María Del Refugio Castañeda-Chávez, Manuel Jesús Chan-Bacab, Ruth Noemí Aguila-Ramírez, Itzel Galaviz-Villa, Pascual Bartolo-Pérez, Fabiola Lango-Reynoso, Carolina Tabasco-Novelo, Christine Gaylarde, Benjamín Otto Ortega-Morales
Cadmium is a major heavy metal found in polluted aquatic environments, mainly derived from industrial production processes. We evaluated the biosorption of solubilized Cd 2+ using the extracellular polymeric substances (EPS) produced by Bacillus sp. MC3B-22 and Microbacterium sp. MC3B-10 (Microbactan); these bacteria were originally isolated from intertidal biofilms off the coast of Campeche, Mexico. EPS were incubated with different concentrations of cadmium in ultrapure water. Residual Cd 2+ concentrations were determined by Inductive Coupled Plasma-Optic Emission Spectrometry and the maximum sorption capacity (Qmax) was calculated according to the Langmuir model...
February 11, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29438575/primary-care-professionals-providing-non-urgent-care-in-hospital-emergency-departments
#12
REVIEW
Daniela Gonçalves-Bradley, Jaspreet K Khangura, Gerd Flodgren, Rafael Perera, Brian H Rowe, Sasha Shepperd
BACKGROUND: In many countries emergency departments (EDs) are facing an increase in demand for services, long waits, and severe crowding. One response to mitigate overcrowding has been to provide primary care services alongside or within hospital EDs for patients with non-urgent problems. However, it is unknown how this impacts the quality of patient care and the utilisation of hospital resources, or if it is cost-effective. This is the first update of the original Cochrane Review published in 2012...
February 13, 2018: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/29434396/structural-and-functional-properties-of-exopolysaccharide-excreted-by-a-novel-bacillus-anthracis-strain-pfab2-of-hot-spring-origin
#13
Aparna Banerjee, Shalini Gaur Rudra, Koushik Mazumder, Vinod Nigam, Rajib Bandopadhyay
Exopolysaccharide produced by a unique avirulent Bacillus anthracis strain PFAB2 of hot spring origin has been characterized and its functional properties are investigated which is a first report. Maximum yield of EPS is 7.66 g/l with 2% glucose and 1% peptone as optimum carbon and nitrogen source respectively. The EPS is found to be a homopolymer consisting of only glucose as principle monosaccharide component. Through 1H NMR study, different dextran-like proton peaks are observed. Molecular weight of the EPS resembles low molecular weight bacterial origin polysaccharides...
March 2018: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/29433934/evidence-based-review-of-pharmacotherapy-for-acute-agitation-part-2-safety
#14
Leslie S Zun
BACKGROUND: The management of acute agitation in the emergency department often requires the administration of rapid-acting antipsychotic agents. However, there are few comparative studies and little guidance regarding the risks associated with use of such drugs in the acute setting. OBJECTIVE: This structured evidence-based review compared the safety of antipsychotic pharmacotherapies for acute agitation using data from randomized controlled trials identified by a literature search of the PubMed database...
February 9, 2018: Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29431296/utility-of-incomplete-right-bundle-branch-block-as-an-isolated-ecg-finding-in-children-undergoing-initial-cardiac-evaluation
#15
Omar Meziab, Dominic J Abrams, Mark E Alexander, Laura Bevilacqua, Vassilios Bezzerides, Doug Y Mah, Edward P Walsh, John K Triedman
OBJECTIVE: This study evaluates the ability of experienced pediatric electrophysiologists (EPs) to reliably classify incomplete right bundle branch block (IRBBB) and assesses its clinical utility as an isolated ECG finding in a group of healthy outpatient children without prior cardiac evaluation. DESIGN: We performed a retrospective analysis of all electrocardiographic and echocardiographic records at Boston Children's Hospital between January 1, 2005, and December 31, 2014...
February 12, 2018: Congenital Heart Disease
https://www.readbyqxmd.com/read/29431142/do-eps-change-their-clinical-behaviour-in-the-hallway-or-when-a-companion-is-present-a-cross-sectional-survey
#16
Hanni Stoklosa, Meredith Scannell, Zheng Ma, Bernard Rosner, Ashley Hughes, J Stephen Bohan
OBJECTIVES: Our aim was to determine whether emergency physicians (EPs) felt their standard patient evaluation practice was modified by two non-private clinical encounters: hallway encounters and encounters during which a companion was present. METHODS: We administered an iteratively developed cross-sectional survey at an annual national professional meeting. We used logistic regression to compare relationships among non-private clinical encounters and predictors of interest...
February 3, 2018: Emergency Medicine Journal: EMJ
https://www.readbyqxmd.com/read/29429709/synergetic-pretreatment-of-waste-activated-sludge-by-hydrodynamic-cavitation-combined-with-fenton-reaction-for-enhanced-dewatering
#17
Meiqiang Cai, Jianqiang Hu, Guanghu Lian, Ruiyang Xiao, Zhijun Song, Micong Jin, Chunying Dong, Quanyuan Wang, Dewen Luo, Zongsu Wei
The dewatering of waste activated sludge by integrated hydrodynamic cavitation (HC) and Fenton reaction was explored in this study. We first investigated the effects of initial pH, sludge concentration, flow rate, and H2O2 concentration on the sludge dewaterability represented by water content, capillary suction time and specific resistance to filtration. The results of dewatering tests showed that acidic pH and low sludge concentration were favorable to improve dewatering performance in the HC/Fenton system, whereas optimal flow rate and H2O2 concentration applied depended on the system operation...
April 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29428762/characterization-of-a-cryptic-plasmid-isolated-from-lactobacillus-casei-cp002616-and-construction-of-shuttle-vectors-based-on-its-replicon
#18
Xin Song, He Huang, Zhiqiang Xiong, Yongjun Xia, Guangqiang Wang, Boxing Yin, Lianzhong Ai
The cryptic plasmid pLC2W was isolated from Lactobacillus casei CP002616. Nucleotide sequence analysis revealed that 4 putative open reading frames (ORF) were responsible for DNA replication. Four Escherichia coli-Lactobacillus shuttle vectors were constructed using different lengths of the pLC2W replicon to identify the shortest functional replicon. The length of the pLC2W replicon did not affect the stability of the plasmids. Green fluorescent protein (GFP) as a reporter was expressed successfully in several lactobacilli using our constructed vectors...
February 7, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29428052/heart-score-and-stress-test-emergency-department-bayesian-decision-scheme-results-from-the-acute-care-diagnostic-collaboration
#19
Naureen Farook, L Cochon, A D Bode, B P Langer, A A Baez
BACKGROUND: Accurate identification of patients at risk of major adverse cardiac events (MACE) places a substantial burden on emergency physicians (EPs). Bayesian nomogram for risk stratification in low- to intermediate-risk cardiovascular patients has not been investigated previously. OBJECTIVE: The objective of this study was to develop a comparative diagnostic model using Bayesian statistics for exercise treadmill test (ETT) and stress echocardiogram (ECHO) to calculate post-test diagnostic risk of MACE using HEART (history, electrocardiogram, age, risk factors, and troponin) risk score as predictor of pretest probability...
February 2018: Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29426120/contaminants-of-emerging-concern-in-the-hartbeespoort-dam-catchment-and-the-umngeni-river-estuary-2016-pollution-incident-south-africa
#20
Cornelius Rimayi, David Odusanya, Jana M Weiss, Jacob de Boer, Luke Chimuka
A quantitative assessment of pollutants of emerging concern in the Hartbeespoort Dam catchment area was conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to establish the occurrence, source and distribution of 15 environmental pollutants, including 10 pharmaceuticals, 1 pesticide and 4 steroid hormones. Seasonal sampling was conducted in the Hartbeespoort Lake using sub-surface grab sampling to determine the lake's ecological status and obtain data for establishment of progressive operational monitoring...
February 2, 2018: Science of the Total Environment
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