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A Fernández-Salas, M A Alonso-Díaz, R A Alonso-Morales, R Lezama-Gutiérrez, J C Rodríguez-Rodríguez, J A Cervantes-Chávez
The acaricidal effects of 55 strains of Metarhizium anisopliae (Metschnikoff) Sorokin, 1883 (Hypocreales: Clavicipitaceae) isolated from paddocks of cattle farms were evaluated in two Rhipicephalus microplus (Canestrini 1887) (Ixodida: Ixodidae) populations, of which one was multi-resistant and one was susceptible to chemical acaricides. Percentage mortality and reproductive efficiency indices in R. microplus were evaluated by adult immersion tests at a dose of 1 × 10(8) conidia/mL for each fungal strain. Some strains were selected to calculate lethal concentrations to kill 50% (LC50 ) and 99% (LC99 ) of engorged ticks...
October 19, 2016: Medical and Veterinary Entomology
Fu-Nan Cho, San-Nung Chen, Yu-Hsiang Chang, James R Carey
OBJECTIVE: Placental site trophoblastic tumor (PSTT) is rare and is characterized by a slow growth. The objective of this report is to present a case of PSTT associated with irregular vaginal spotting that occurred 1 year after normal vaginal delivery. CASE REPORT: This report provides interesting ultrasound, hysteroscopy, and histology findings of PSTT. It is difficult to make a clinical diagnosis of PSTT at an early stage. Without the use of immunohistochemical analysis, PSTT may evade histological detection...
October 2016: Taiwanese Journal of Obstetrics & Gynecology
Liangliang Xiang, Błażej Poźniak, Tian-Yin Cheng
Tick-borne diseases are a major epidemiological problem worldwide. The aim of this study was to investigate the bacterial composition of saliva obtained from engorged adult Rhipicephalus microplus females. Saliva samples collected from partially or fully engorged adult female ticks were analysed using an ultra-high-throughput Illumina HiSeq 2500 sequencing system. To elucidate the possible routes of bacterial transmission, the bacterial flora from whole ticks were also investigated. Proteobacteria, Firmicutes, and Actinobacteria were the predominant phyla in all samples, and Acinetobacter, Rickettsia, Escherichia and Coxiella were the major genera...
October 15, 2016: Antonie Van Leeuwenhoek
Rintaro Yokoyama, Satoshi Iihoshi, Kei Miyata, Kentaro Toyama, Katsuya Komatsu, Masahiko Wanibuchi, Nobuhiro Mikuni
OBJECT: We report a relatively rare case of pediatric iatrogenic extradural vertebral arteriovenous fistula. CASE: A five-year-old boy with a history of multiple surgeries for Total Anomalous Pulmonary Venous Return(TAPVR)exhibited vascular engorgement of his right neck from about 4 years old. We thought that this was caused by his past operations. He demonstrated swollen blood vessels in the right neck and pulmonary hypertension with increased right heart load...
October 2016: No Shinkei Geka. Neurological Surgery
Deborah C Jaworski, Chuanmin Cheng, Arathy D S Nair, Roman R Ganta
Monocytic ehrlichiosis in people caused by the intracellular bacterium, Ehrlichia chaffeensis, is an emerging infectious disease transmitted by the lone star tick, Amblyomma americanum. Tick transmission disease models for ehrlichiosis require at least two hosts and two tick blood feeding episodes to recapitulate the natural transmission cycle. One blood feeding is necessary for the tick to acquire the infection from an infected host and the next feeding is needed to transmit the bacterium to a naïve host...
September 28, 2016: Ticks and Tick-borne Diseases
Quraish Ghadiali, Anna Tan, K Bailey Freund
PURPOSE: We describe a patient with an unusually posterior varix of a vortex vein ampulla. METHODS: Case report. RESULTS: An unusually posterior varix of a vortex vein ampulla mimicking a choroidal neoplasm was identified. The diagnosis was obscured by the presence of overlying drusen. Through a combination of indocyanine green angiography and enhanced depth imaging optical coherence tomography, the varix was identified as an engorged vascular process correlating to ophthalmoscopic and tomographic examination...
August 23, 2016: Retinal Cases & Brief Reports
Bosheng He, Jinhua Gu, Sheng Huang, Xuesong Gao, Jinhe Fan, Meihong Sheng, Lin Wang, Shenchu Gong
INTRODUCTION: This study was performed to evaluate the diagnostic performance of multi-slice CT angiography combined with enterography in determining the cause and location of obstruction as well as intestinal ischaemia in patients with small bowel obstruction (SBO). METHODS: This study retrospectively summarized the image data of 57 SBO patients who received both multi-slice CT angiography and enterography examination between December 2012 and May 2013. The CT diagnoses of SBO and intestinal ischaemia were correlated with the findings at surgery or digital subtraction angiography, which were set as standard references...
October 5, 2016: Journal of Medical Imaging and Radiation Oncology
Luís Adriano Anholeto, Patrícia Rosa de Oliveira, Rodney Alexandre Ferreira Rodrigues, Caroline Dos Santos Spindola, Marcelo Bahia Labruna, Marcos Aparecido Pizano, Karina Neoob de Carvaldo Castro, Maria Izabel Camargo-Mathias
The use of synthetic acaricides is currently the main method to control ticks. However, the indiscriminate use of these chemicals can lead to the selection of resistant individuals and in the accumulation of chemical residues in the environment, contaminating the soil and water streams, consequently affecting the flora, fauna, and the human beings as well. In this sense, the objective of this study was to investigate the acaricidal effect of crude ethanolic extract of Acmella oleracea (L.) R.K. Jansen aerials parts at different concentrations on fed males and semi-engorged females of A...
September 30, 2016: Ticks and Tick-borne Diseases
Setareh Jahfari, Agnetha Hofhuis, Manoj Fonville, Joke van der Giessen, Wilfrid van Pelt, Hein Sprong
BACKGROUND: Tick-borne diseases are the most prevalent vector-borne diseases in Europe. Knowledge on the incidence and clinical presentation of other tick-borne diseases than Lyme borreliosis and tick-borne encephalitis is minimal, despite the high human exposure to these pathogens through tick bites. Using molecular detection techniques, the frequency of tick-borne infections after exposure through tick bites was estimated. METHODS: Ticks, blood samples and questionnaires on health status were collected from patients that visited their general practitioner with a tick bite or erythema migrans in 2007 and 2008...
October 2016: PLoS Neglected Tropical Diseases
Fabrício M Alves, Cíntia C Bernardo, Flávia R S Paixão, Lucas P Barreto, Christian Luz, Richard A Humber, Éverton K K Fernandes
The current study investigated the thermotolerance of Metarhizium anisopliae s.l. conidia from the commercial products Metarril® SP Organic and Metarril® WP. The efficacy of these M. anisopliae formulations against the tick Rhipicephalus sanguineus s.l. was studied in laboratory under optimum or heat-stress conditions. The products were prepared in water [Tween® 80, 0.01 % (v/v)] or pure mineral oil. Conidia from Metarril® SP Organic suspended in water presented markedly delayed germination after heating to constant 40 °C (for 2, 4, or 6 h) compared to conidia suspended in mineral oil...
October 4, 2016: Parasitology Research
Minako Kaido, Osama M A Ibrahim, Motoko Kawashima, Reiko Ishida, Enrique A Sato, Kazuo Tsubota
PURPOSE: To investigate the efficacy of eyelid margin cleansing with ofloxacin ophthalmic ointment in obstructive meibomian gland dysfunction (MGD) patients. METHODS: Thirty-one eyes of 31 patients diagnosed with obstructive MGD were enrolled. All subjects were instructed to rub the eyelid edge with ofloxacin eye ointment once daily prior to bathing. Lid margin abnormalities, meibum properties, and tear stability were observed before and 3 months after ointment treatment...
October 3, 2016: Japanese Journal of Ophthalmology
Gilian van Duijvendijk, Wouter van Andel, Manoj Fonville, Gerrit Gort, Joppe W Hovius, Hein Sprong, Willem Takken
Several microorganisms have been shown to manipulate their host or vector to enhance their own transmission. Here we examined whether an infection with Borrelia afzelii affects its transmission between its bank vole (Myodes glareolus, Schreber, 1780) host and tick vector. Captive-bred bank voles were inoculated with B. afzelii or control medium, after which host preference of Ixodes ricinus L. nymphs was determined in a Y-tube olfactometer. Thereafter, infected and uninfected bank voles were placed in a semifield arena containing questing larvae to measure larval tick attachment...
September 30, 2016: Journal of Medical Entomology
Paula Barroso Cruz, Alan Franco Barbosa, Viviane Zeringóta, Diego Melo, Tatiane Novato, Queli Cristina Fidelis, Rodrigo Luiz Fabri, Mário Geraldo de Carvalho, Armando Ubirajara Oliveira Sabaa-Srur, Erik Daemon, Caio Márcio Oliveira Monteiro
We evaluated the acaricidal activity of Acmella oleracea methanol extract and spilanthol on Rhipicephalus microplus and Dermacentor nitens. The extract was made through maceration with methanol. From this extract, a dichloromethane fraction with 99% spilanthol was obtained and tested on R. microplus larvae and engorged females and D. nitens larvae. For evaluation against larvae, the modified larval packet test was used, and both the methanol extract and dichloromethane fraction were tested at concentrations of 0...
September 15, 2016: Veterinary Parasitology
Genevieve E Becker, Hazel A Smith, Fionnuala Cooney
BACKGROUND: Breastfeeding is important, however not all infants can feed at the breast and methods of expressing milk need evaluation. OBJECTIVES: To assess acceptability, effectiveness, safety, effect on milk composition, contamination and costs of methods of milk expression. SEARCH METHODS: We searched the Cochrane Pregnancy and Childbirth Group's Trials Register (21 March 2016), handsearched relevant journals and conference proceedings, and contacted experts in the field to seek additional published or unpublished studies...
September 29, 2016: Cochrane Database of Systematic Reviews
Mustapha Dahmani, Bernard Davoust, Frédéric Rousseau, Didier Raoult, Florence Fenollar, Oleg Mediannikov
Rhipicephalus bursa is one of 79 species of the genus Rhipicephalus in the family of Ixodidae. In this study, we investigated Anaplasmataceae bacteria associated with R. bursa collected after an epizootic outbreak of ovine anaplasmosis. 76 adult ticks, (60 male and 16 female ticks), were removed from sheep in two farms and all identified as R. bursa, all females were partially engorged. We found that 50% of the ticks were positive in the initial Anaplasmataceae qPCR screening. Bacterial species was identified by analyzing the sequences of amplicons of 23S rRNA, groEL and rpoB genes...
September 19, 2016: Ticks and Tick-borne Diseases
Lei Liu, Tian-Yin Cheng, Fen Yan
Tick-borne-diseases (TBD) pose a huge threat to the health of both humans and animals worldwide. Tick vaccines constitute an attractive alternative for tick control, due to their cost-efficiency and environmental-friendliness. Subolesin, a protective antigen against ticks, is reported to be a promising candidate for the development of broad-spectrum vaccines. However, the entire length of its gene, subA, and its gene expression pattern in different tissues and blood-feeding status (or different levels of engorgement) have not been studied extensively...
September 15, 2016: Experimental & Applied Acarology
Ignatius Ngene Esene, Ahmed M Ashour, Eric Marvin, Mohamed Nosseir, Zeiad Y Fayed, Khaled Seoud, Khaled El Bahy
INTRODUCTION: Pure spinal epidural cavernous hemangiomas (PSECHs) are rare vascular lesions with about 100 cases reported. Herein, we present a case series of 7 PSECHs discussing their clinical presentation, radiological characteristics, surgical technique and intraoperative findings, pathological features, and functional outcome. MATERIALS AND METHODS: We retrieved from the retrolective databases of the senior authors, patients with pathologically confirmed PSECH operated between January 2002 and November 2015...
July 2016: Journal of Craniovertebral Junction and Spine
Glenn Isaacson
Acute otitis media can be difficult to diagnose in an uncooperative, crying child. The vessels of the external auditory canal and tympanic membrane can engorge simulating acute otitis media. We examined and photographed the ears of children and young adults during a modified Valsalva maneuver to document the pattern of vascular congestion. Absent signs of middle ear effusion, lack of true tympanic membrane bulging, and normal pneumatic otoscopy help to differentiate the appearance of a crying child's ear from that seen in acute bacterial otitis media...
September 12, 2016: Pediatric Infectious Disease Journal
Peter James Krause, Jonathan Schwab, Sukanya Narasimhan, Janna Brancato, Guang Xu, Stephen M Rich
A 5-year old Massachusetts resident developed hard tick-borne relapsing fever caused by Borrelia miyamotoi. A partially engorged Ixodes scapularis tick was removed from her scalp and identified as infected with B. miyamotoi using PCR. Two weeks later, she developed an illness compatible with B. miyamotoi infection that included fatigue and recurrent fever. The diagnosis was confirmed by B. miyamotoi seroconversion.
September 12, 2016: Pediatric Infectious Disease Journal
Gabriel Cerqueira Alves Costa, Adriana Coelho Soares, Marcos Horácio Pereira, Nelder Figueiredo Gontijo, Maurício Roberto Viana Sant'Anna, Ricardo Nascimento Araujo
Ornithodoros rostratus is an argasid tick and its importance is based on its hematophagy and the resulting transmission of pathogens such as Rickettsia rickettsii and Coxiella burnetii unto its vertebrate hosts. In the face of a lack of physiological studies related to hematophagy in argasid ticks, this paper aims to identify and characterize the events that occur throughout the feeding by O. rostratus on live hosts. Electrical signals and alterations on the feeding site were monitored using intravital microscopy and electromyography...
September 13, 2016: Journal of Experimental Biology
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