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https://www.readbyqxmd.com/read/28717309/molecular-characterization-of-antibiotic-resistant-staphylococcus-aureus-from-livestock-bovine-and-swine
#1
Asima Zehra, Randhir Singh, Simranpreet Kaur, J P S Gill
AIM: The aim of this study was to figure the prevalence, phenotypic and genotypic antibiotic resistance (AR) pattern of Staphylococcus aureus isolated from bovine and swine nares. MATERIALS AND METHODS: Colonies with typical morphology on Baird-Parker agar supplemented with egg-yolk tellurite emulsion were selected and biochemically/genotypically identified as S. aureus. These strains were further subjected to epsilometer test for their sensitivity to various clinically important antibiotics and antibiotic susceptibility testing for amoxicillin/clavulanic acid, and double-disk diffusion testing was performed by the standard disc diffusion method following CLSI guidelines...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28713454/balance-basic-anthropometrics-and-performance-in-young-alpine-skiers-longitudinal-analysis-of-the-associations-during-two-competitive-seasons
#2
Blaz Lesnik, Damir Sekulic, Matej Supej, Michael R Esco, Milan Zvan
Balance is hypothesized to be important in alpine skiing, while it is known that balance depends on anthropometric indices. The aim of this investigation was to explore the association between balance, anthropometrics and skiing-results over two competitive seasons among youth alpine-skiers. Eighty-one skiers (40 females) participated in this study. The participants were tested twice over two competitive seasons: when they were 12-13 years old (U14) and when they were 14-15 years old (U16). The variables consisted of anthropometrics (body height and body mass) and three balance indexes (medio-lateral-, anterio-posterior- and overall-stability-index)...
June 2017: Journal of Human Kinetics
https://www.readbyqxmd.com/read/28669380/acute-kidney-injury-in-critically-ill-adult-patients-with-seasonal-influenza-infection
#3
Ana Dovč, Vladimir Premru, Blaž Pečavar, Rafael Ponikvar
BACKGROUND: Acute kidney injury (AKI) occurs in 22.4 - 100% of critically-ill hospitalized patients with influenza infection. In up to 2/3, it is severe enough to necessitate renal replacement therapy. We aimed to document the incidence of AKI among patients with influenza-related critical illness and its relation to clinical outcomes. METHODS: We conducted a retrospective observational study of all adult patients with acute respiratory illness and laboratory-confirmed influenza infection admitted to non-surgical intensive care units at the University Medical Centre Ljubljana between January 1, 2016, and March 31, 2016...
July 2, 2017: Clinical Nephrology
https://www.readbyqxmd.com/read/28647622/risk-factors-for-unsuccessful-acetabular-press-fit-fixation-at-primary-total-hip-arthroplasty
#4
Urban Brulc, Vane Antolič, Blaž Mavčič
BACKGROUND: Surgeon at primary total hip arthroplasty sometimes cannot achieve sufficient cementless acetabular press-fit fixation and must resort to other fixation methods. Despite a predominant use of cementless cups, this issue is not fully clarified, therefore we performed a large retrospective study to: 1) identify risk factors related to patient or implant or surgeon for unsuccessful intraoperative press-fit, 2) check for correlation between surgeons' volume of operated cases and the press-fit success rate...
June 21, 2017: Orthopaedics & Traumatology, Surgery & Research: OTSR
https://www.readbyqxmd.com/read/28621381/the-methodology-applied-in-dpph-abts-and-folin-ciocalteau-assays-has-a-large-influence-on-the-determined-antioxidant-potential
#5
Helena Abramovič, Blaž Grobin, Nataša Poklar, Blaž Cigić
Antioxidant potential (AOP) is not only the property of the matrix analyzed but also depends greatly on the methodology used. The chromogenic radicals 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and Folin-Ciocalteu (FC) assay were applied to estimate how the method and the composition of the assay solvent influence the AOP determined for coffee, tea, beer, apple juice and dietary supplements. Large differences between the AOP values depending on the reaction medium were observed, with the highest AOP determined mostly in the FC assay...
June 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/28588310/the-development-of-fluorescent-protein-tracing-vectors-for-multicolor-imaging-of-clinically-isolated-staphylococcus-aureus
#6
Fuminori Kato, Motoki Nakamura, Motoyuki Sugai
Recent advances in fluorescent protein technology provide a wide variety of biological imaging applications; however current tools for bio-imaging in the Gram-positive bacterium Staphylococcus aureus has necessitated further developments for fluorescence intensity and for a multicolor palette of fluorescent proteins. To enhance the expression of multicolor fluorescent proteins in clinical S. aureus strains, we developed new fluorescent protein expression vectors, containing the blaZ/sodp promoter consisting of the β-lactamase gene (blaZ) promoter and the ribosome binding site (RBS) of superoxide dismutase gene (sod)...
June 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28578698/dictyexpress-a-web-based-platform-for-sequence-data-management-and-analytics-in-dictyostelium-and-beyond
#7
Miha Stajdohar, Rafael D Rosengarten, Janez Kokosar, Luka Jeran, Domen Blenkus, Gad Shaulsky, Blaz Zupan
BACKGROUND: Dictyostelium discoideum, a soil-dwelling social amoeba, is a model for the study of numerous biological processes. Research in the field has benefited mightily from the adoption of next-generation sequencing for genomics and transcriptomics. Dictyostelium biologists now face the widespread challenges of analyzing and exploring high dimensional data sets to generate hypotheses and discovering novel insights. RESULTS: We present dictyExpress (2.0), a web application designed for exploratory analysis of gene expression data, as well as data from related experiments such as Chromatin Immunoprecipitation sequencing (ChIP-Seq)...
June 2, 2017: BMC Bioinformatics
https://www.readbyqxmd.com/read/28531417/obesity-in-the-elderly-weight-loss-yes-or-no
#8
Milena Kovač Blaž
No abstract text is available yet for this article.
August 2016: Clinical Nutrition ESPEN
https://www.readbyqxmd.com/read/28522975/hypoxia-and-inactivity-related-physiological-changes-constipation-inflammation-are-not-reflected-at-the-level-of-gut-metabolites-and-butyrate-producing-microbial-community-the-planhab-study
#9
Robert Šket, Nicole Treichel, Tadej Debevec, Ola Eiken, Igor Mekjavic, Michael Schloter, Marius Vital, Jenna Chandler, James M Tiedje, Boštjan Murovec, Zala Prevoršek, Blaž Stres
We explored the assembly of intestinal microbiota in healthy male participants during the run-in (5 day) and experimental phases [21-day normoxic bed rest (NBR), hypoxic bedrest (HBR)], and hypoxic ambulation (HAmb) in a strictly controlled laboratory environment, balanced fluid, and dietary intakes, controlled circadian rhythm, microbial ambiental burden, and 24/7 medical surveillance. The fraction of inspired O2 (FiO2) and partial pressure of inspired O2 (PiO2) were 0.209 and 133.1 ± 0.3 mmHg for NBR and 0...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28498966/genprobis-web-server-for-mapping-of-sequence-variants-to-protein-binding-sites
#10
Janez Konc, Blaz Skrlj, Nika Erzen, Tanja Kunej, Dusanka Janezic
Discovery of potentially deleterious sequence variants is important and has wide implications for research and generation of new hypotheses in human and veterinary medicine, and drug discovery. The GenProBiS web server maps sequence variants to protein structures from the Protein Data Bank (PDB), and further to protein-protein, protein-nucleic acid, protein-compound, and protein-metal ion binding sites. The concept of a protein-compound binding site is understood in the broadest sense, which includes glycosylation and other post-translational modification sites...
May 11, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28497118/novel-%C3%AE-lactamase-blaarl-in-staphylococcus-arlettae
#11
Sabrina N Andreis, Vincent Perreten, Sybille Schwendener
Whole-genome sequencing of penicillin-resistant Staphylococcus arlettae strain SAN1670 from bovine mastitis milk revealed a novel β-lactamase operon consisting of the β-lactamase-encoding gene blaARL, the antirepressor-encoding gene blaR1ARL, and the repressor-encoding gene blaIARL. The functionality of blaARL was demonstrated by gene expression in Staphylococcus aureus. The blaARL operon was chromosomally located in SAN1670 and present in 10 additional unrelated strains, suggesting intrinsic penicillin resistance in S...
May 2017: MSphere
https://www.readbyqxmd.com/read/28478822/animal-nutrition-and-breeding-conditions-modify-the-physiology-of-isolated-primary-cells
#12
Irina Milisav, Blaž Banič, Dušan Šuput
Animal primary cell cultures are widely used in biomedical research to investigate cell metabolism, diseases and to devise novel treatments. Modern animal breeding techniques are developed to unify, control and reduce the amount of microorganisms that the animals are being exposed to. Furthermore, health monitoring and strict caging and handling protocols allow animals to be exposed only to a selected spectrum of microbes. We are starting to appreciate that nutrition can influence composition of gut microbiota that can impact hosting organism's physiology and can even result in development of pathological changes...
May 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28470080/genetic-characterisation-of-antimicrobial-resistance-and-virulence-genes-in-i-staphylococcus-aureus-i-isolated-from-commercial-broiler-chickens-in-the-durban-metropolitan-area-south-africa
#13
Nelisiwe Mkize, Oliver T Zishiri, Samson Mukaratirwa
Antimicrobial resistance of Staphylococcus aureus in human and veterinary medicine is a serious worldwide problem. The aim of this study was to investigate the prevalence of S. aureus in commercial broiler chickens as well as to establish antimicrobial susceptibility and the distribution of genetic determinants conferring resistance and virulence. One hundred and ninety-four samples were aseptically collected from broiler chicken slaughterhouses and retail outlets around the Durban metropolitan area in South Africa...
May 4, 2017: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/28452122/identification-of-sequence-variants-within-experimentally-validated-protein-interaction-sites-provides-new-insights-into-molecular-mechanisms-of-disease-development
#14
Blaz Skrlj, Janez Konc, Tanja Kunej
Protein interactions (PI) underlie complex biological processes. Protein interaction partners include DNA, RNA, ions, small chemical compounds, and proteins (protein-protein interactions; PPI). Analysis of sequence variants within regions corresponding to experimentally validated PI sites presents novel opportunities for understanding of complex diseases. Such information has not been systematically collected due to the fact that datasets are dispersed throughout databases and publications. Sequence variants and PI regions were obtained from the UniProt database...
April 28, 2017: Molecular Informatics
https://www.readbyqxmd.com/read/28400004/effect-of-food-related-stress-conditions-and-loss-of-agr-and-sigb-on-seb-promoter-activity-in-s-%C3%A2-aureus
#15
Henna-Maria Sihto, Roger Stephan, Christoph Engl, John Chen, Sophia Johler
Staphylococcal enterotoxin B (SEB) causes staphylococcal food poisoning and is produced in up to ten times higher quantities than other major enterotoxins. While Staphylococcus aureus growth is often repressed by competing flora, the organism exhibits a decisive growth advantage under some stress conditions. So far, data on the influence of food-related stressors and regulatory mutations on seb expression is limited and largely based on laboratory strains, which were later reported to harbor mutations. Therefore, the aim of this study was to investigate the influence of stress and regulatory mutations on seb promoter activity...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28375940/electrophysiological-studies-to-detect-peripheral-neuropathy-in-children-treated-with-vincristine
#16
Marko Kavcic, Blaz Koritnik, Matevz Krzan, Orjana Velikonja, Tomaz Prelog, Milica Stefanovic, Maruša Debeljak, Janez Jazbec
Patients treated with vincristine predictably develop peripheral neuropathy. The aim of our study was to investigate the pattern of vincristine-induced neuropathy in children by nerve conduction studies and somatosensory-evoked potentials (SSEPs). We included data from 39 children who received vincristine for various pediatric malignancies, and we performed initial and follow-up (after a minimum of 4 doses of vincristine 1.5 mg/m) conduction studies in 27 patients and SSEPs studies in 34 patients. On follow-up the most prevalent symptoms were paresthesias (44%) and constipation (22%), and the most common neurological sign was impaired myotatic reflexes (89%)...
May 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28363998/e3-ligase-ciap2-mediates-downregulation-of-mre11-and-radiosensitization-in-response-to-hdac-inhibition-in-bladder-cancer
#17
Judith Nicholson, Sarah J Jevons, Blaz Groselj, Sophie Ellermann, Rebecca Konietzny, Martin Kerr, Benedikt M Kessler, Anne E Kiltie
The MRE11/RAD50/NBS1 (MRN) complex mediates DNA repair pathways, including double-strand breaks induced by radiotherapy. Meiotic recombination 11 homolog (MRE11) is downregulated by histone deacetylase inhibition (HDACi), resulting in reduced levels of DNA repair in bladder cancer cells and radiosensitization. In this study, we show that the mechanism of this downregulation is posttranslational and identify a C-terminally truncated MRE11, which is formed after HDAC inhibition as full-length MRE11 is downregulated...
March 31, 2017: Cancer Research
https://www.readbyqxmd.com/read/28355437/radiofrequency-exposures-of-workers-on-low-power-fm-radio-transmitters
#18
Blaž Valic, Bor Kos, Peter Gajšek
Low-power radio transmitters are one of the most common radio frequency sources and the exposure limit values (ELVs) for occupational exposure may be exceeded close to them. Therefore, a detailed analysis and assessment of occupational exposure in their vicinity is presented in the paper. For 20 different exposure scenarios, electric field strength and specific absorption rate (SAR) values were computed to determine whether the action levels (ALs) and ELVs of the European directive 2013/35/EU are exceeded for different 500 W radio transmitters...
May 1, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/28346685/experience-of-shame-mediates-the-relationship-between-pathological-narcissism-and-suicidal-ideation-in-psychiatric-outpatients
#19
Nenad Jaksic, Darko Marcinko, Milena Skocic Hanzek, Blaz Rebernjak, John S Ogrodniczuk
OBJECTIVE: Pathological narcissism, described by 2 dysfunctional phenotypic forms-grandiosity and vulnerability-has often been connected to suicidal tendencies in theoretical and clinical literatures. Furthermore, shame proneness has been implicated as a key mechanism that links these 2 constructs. However, empirical evidence for the presumed relationship between pathological narcissism and suicidal tendencies is sparse, and no prior research has investigated the role of shame proneness in this relationship...
March 27, 2017: Journal of Clinical Psychology
https://www.readbyqxmd.com/read/28339104/transferable-antibiotic-resistances-in-marketed-edible-grasshoppers-locusta-migratoria-migratorioides
#20
Andrea Osimani, Cristiana Garofalo, Lucia Aquilanti, Vesna Milanović, Federica Cardinali, Manuela Taccari, Marina Pasquini, Stefano Tavoletti, Francesca Clementi
Grasshoppers are the most commonly eaten insects by humans worldwide, as they are rich in proteins and micronutrients. This study aimed to assess the occurrence of transferable antibiotic resistance genes in commercialized edible grasshoppers. To this end, the prevalence of 12 selected genes [aac(6')-Ie aph(2″)-Ia, blaZ, erm(A), erm(B), erm(C), mecA, tet(M), tet(O), tet(S), tet(K), vanA, vanB] coding for resistance to antibiotics conventionally used in clinical practice was determined. The majority of samples were positive for tet(M) (70...
May 2017: Journal of Food Science
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