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https://www.readbyqxmd.com/read/28087612/biofilm-formation-and-antimicrobial-resistance-genes-of-coagulase-negative-staphylococci-isolated-from-cows-with-mastitis-in-argentina
#1
Mariela E Srednik, Yannick D N Tremblay, Josée Labrie, Marie Archambault, Mario Jacques, Fernández Cirelli Alicia, Elida R Gentilini
Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008-2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation and Staphylococcus haemolyticus species formed the strongest biofilms...
January 12, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28069883/comparison-of-phenotypic-methods-for-the-detection-of-penicillinase-in-staphylococcus-aureus-and-proposal-of-a-practical-diagnostic-approach
#2
Michael Hombach, Christoph Weissert, Maria Magdalena Senn, Reinhard Zbinden
OBJECTIVES: Disc diffusion is a cost-efficient, low-complexity, reliable method for detection of blaZ-mediated benzylpenicillin resistance in Staphylococcus aureus if the zone edge is inspected. EUCAST breakpoints cannot fully separate β-lactamase-positive from β-lactamase-negative strains, and EUCAST recommends the zone edge test. Literature on nitrocefin-based testing and the zone edge test is scarce with wide variations in reported assay performance. METHODS: This study compared two different nitrocefin-based commercial and in-house tests and the EUCAST-based zone edge test for penicillinase detection in S...
January 8, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28004151/reciprocal-regulation-of-mtor-complexes-in-pancreatic-islets-from-humans-with-type-2-diabetes
#3
Ting Yuan, Sahar Rafizadeh, Kanaka Durga Devi Gorrepati, Blaz Lupse, Jose Oberholzer, Kathrin Maedler, Amin Ardestani
AIMS/HYPOTHESIS: Mechanistic target of rapamycin complex 1 (mTORC1) is a master regulator of nutritional status at the cellular and organismic level. While mTORC1 mediates beta cell growth and expansion, its hyperactivation has been observed in pancreatic islets from animal models of type 2 diabetes and leads to beta cell loss. We sought to determine whether such mTORC1 activation occurs in humans with type 2 diabetes or in metabolically stressed human islets and whether mTORC1 blockade can restore beta cell function of diabetic islets...
December 21, 2016: Diabetologia
https://www.readbyqxmd.com/read/27992232/optically-detected-degradation-of-nayf4-yb-tm-based-upconversion-nanoparticles-in-phosphate-buffered-saline-solution
#4
Olivija Plohl, Marco Kraft, Janez Kovač, Blaž Belec, Maja Ponikvar-Svet, Christian Würth, Darja Lisjak, Ute Resch-Genger
In a proof-of-concept study, we assessed different analytical and spectroscopic parameters for stability screening of differently sized β-NaYF4:20 mol % Yb(3+), 2 mol % Tm(3+) upconversion nanoparticles (UCNPs) exemplarily in the bioanalytically relevant buffer phosphate buffered saline (PBS; pH 7.4) at 37 and 50 °C. This included the potentiometric determination of the amount of released fluoride ions, surface analysis with X-ray photoelectron spectroscopy (XPS), and steady-state and time-resolved fluorescence measurements...
January 3, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27957840/all-solid-state-reference-electrode-with-heterogeneous-membrane
#5
Andrzej Lewenstam, Teresa Blaz, Jan Migdalski
Novel reference electrodes with a solid contact coated by a heterogeneous polymer membrane are described. The electrodes are obtained using Ag nanoparticles, AgBr, KBr suspended in tetrahydrofuran solution of PVC and DOS and deposited on Ag substrate, or another substrate covered with Ag, by drop casting. After a short period of soaking in a KBr solution, stable and reproducible formal potentials of -157 ± 2 mV (vs Ag/AgCl/3 M KCl) were observed, and the solid-contact reference electrodes were ready to use...
December 22, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27928206/doping-attitudes-and-covariates-of-potential-doping-behaviour-in-high-level-team-sport-athletes-gender-specific-analysis
#6
Damir Sekulic, Enver Tahiraj, Milan Zvan, Natasa Zenic, Ognjen Uljevic, Blaz Lesnik
Team sports are rarely studied with regard to doping behaviour and doping-related factors regardless of their global popularity. This study aimed to investigate doping factors and covariates of potential doping behaviour in high-level team-sport athletes. The subjects were 457 high-performing, national- and international-level athletes (21.9 ± 3.4 years of age; 179 females) involved in volleyball (n = 77), soccer (n = 163), basketball (n = 114) and handball (n = 103). Previously validated self-administered questionnaires aimed at evidencing sport factors, doping-related factors, knowledge on sport nutrition and doping, and attitudes to performance enhancement were used...
December 2016: Journal of Sports Science & Medicine
https://www.readbyqxmd.com/read/27927926/clinical-and-microbiological-aspects-of-%C3%AE-lactam-resistance-in-staphylococcus-lugdunensis
#7
Ian H McHardy, Jennifer Veltman, Janet Hindler, Katia Bruxvoort, Marissa M Carvalho, Romney M Humphries
Antimicrobial susceptibility results from broth microdilution MIC testing of 993 S. lugdunensis isolates recovered from patients at a tertiary care medical center from 2008-2015 were reviewed. Ninety-two oxacillin-susceptible isolates were selected to assess the accuracy of penicillin MIC, penicillin disk diffusion and three β-lactamase tests, including the cefoxitin-induced nitrocefin test, penicillin cloverleaf assay and penicillin disk zone-edge test. Results of all phenotypic tests were compared to blaZ PCR...
December 7, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27913076/biofilm-production-and-beta-lactamic-resistance-in-brazilian-staphylococcus-aureus-isolates-from-bovine-mastitis
#8
Viviane Figueira Marques, Cássia Couto da Motta, Bianca da Silva Soares, Dayanne Araújo de Melo, Shana de Mattos de Oliveira Coelho, Irene da Silva Coelho, Helene Santos Barbosa, Miliane Moreira Soares de Souza
Staphylococcus spp. play an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant species due to the production of virulence factors such as slime, which is required for biofilm formation. This study aimed to evaluate biofilm production and its possible relation to beta-lactamic resistance in 20 S. aureus isolates from bovine mastitic milk. The isolates were characterized by pheno-genotypic and MALDI TOF-MS assays and tested for genes such as icaA, icaD, bap, agr RNAIII, agr I, agr II, agr III, and agr IV, which are related to slime production and its regulation...
January 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27904413/influence-of-yellow-light-emitting-diodes-at-590-nm-on-storage-of-apple-tomato-and-bell-pepper-fruit
#9
Doris Kokalj, Janez Hribar, Blaž Cigić, Emil Zlatić, Lea Demšar, Lovro Sinkovič, Helena Šircelj, Grega Bizjak, Rajko Vidrih
The objective of this study is to investigate the effects of irradiation from light-emitting diodes (LEDs) on several fruits during storage. To improve storage and increase the contents of some bioactive compounds, apple, tomato and red bell pepper fruits were exposed to yellow light emitted from the diodes at 590 nm. The contents of ascorbic acid, total phenolics, total flavonoids and several pigments were investigated, along with the antioxidant potential. The colour parameters (L*, a* and b*) and firmness of the fruit were also determined...
June 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/27889420/correlation-of-phenotypic-tests-with-the-presence-of-the-blaz-gene-for-detection-of-beta-lactamase
#10
Adriano Martison Ferreira, Katheryne Benini Martins, Vanessa Rocha da Silva, Alessandro Lia Mondelli, Maria de Lourdes Ribeiro de Souza da Cunha
Staphylococcus aureus and Staphylococcus saprophyticus are the most common and most important staphylococcal species associated with urinary tract infections. The objective of the present study was to compare and to evaluate the accuracy of four phenotypic methods for the detection of beta-lactamase production in Staphylococcus spp. Seventy-three strains produced a halo with a diameter ≤28mm (penicillin resistant) and all of them were positive for the blaZ gene. Among the 28 susceptible strain (halo ≥29mm), 23 carried the blaZ gene and five did not...
January 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27867460/percutaneous-dual-valve-intervention-in-a-high-risk-patient-with-severe-aortic-and-mitral-stenosis
#11
Blaz Mrevlje, Mohamad Aboukura, Christoph A Nienaber
Aortic stenosis is the most frequent and mitral stenosis is the least frequent native single-sided valve disease in Europe. Patients with the combination of severe symptomatic degenerative aortic and mitral stenosis are very rare. Guidelines for the treatment of heart valve diseases are clear for single-valve situations. However, there is no common agreement or recommendation for the best treatment strategy in patients with multiple valve disease and severe concomitant comorbidities. A 76-year-old female patient with the combination of severe degenerative symptomatic aortic and mitral stenosis and several comorbidities including severe obesity, who was found unsuitable surgical candidate by the heart team and unsuitable for two-time general anesthesia in the case of two-step single-valve percutaneous approach by anesthesiologists, underwent successful percutaneous dual-valve single-intervention (transcatheter aortic valve implantation and percutaneous mitral balloon commissurotomy)...
July 2016: Heart Views: the Official Journal of the Gulf Heart Association
https://www.readbyqxmd.com/read/27861199/heart-rate-reduction-with-adjusted-dose-ivabradine-in-patients-undergoing-coronary-computed-tomographic-angiography-a-randomized-trial
#12
Blaž Kacijan, Zala Novak, Borut Jug, Maja Dolenc Novak, Matjaž Vrtovec, Barbara Gužič Salobir
OBJECTIVE: Our prospective, randomized, open-label study assessed the efficacy of a heart rate-lowering, adjusted-dose protocol with ivabradine prior to coronary computed tomographic angiography (CCTA). METHODS: Patients undergoing CCTA were randomized to 7 days of adjusted-dose ivabradine or standard care (ie, no additional medication). Heart rate and β-blocker and antianxiety medication use on the day of the CCTA were recorded. RESULTS: One hundred one patients were randomized (mean age, 60 [SD, 13] years; 66% women)...
November 17, 2016: Journal of Computer Assisted Tomography
https://www.readbyqxmd.com/read/27815733/acute-rheumatic-fever-outbreak-in-southern-central-european-country
#13
Urška Kočevar, Nataša Toplak, Blaž Kosmač, Luka Kopač, Samo Vesel, Natalija Krajnc, Matjaž Homan, Rina Rus, Tadej Avčin
: A decline in the incidence of acute rheumatic fever (ARF) in developed countries over the past century can be attributed to the improved public hygiene and to widespread use of antibiotics. ARF seemed to be a rare disease in southern central European country, Slovenia, up to 2010 when we noticed an increase in the number of patients with ARF. In order to assess the current incidence of ARF, we performed a retrospective study of all patients with ARF treated at the University Children's Hospital Ljubljana from January 2008 until the end of December 2014...
January 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/27812538/proproliferative-and-antiapoptotic-action-of-exogenously-introduced-yap-in-pancreatic-%C3%AE-cells
#14
Ting Yuan, Sahar Rafizadeh, Zahra Azizi, Blaz Lupse, Kanaka Durga Devi Gorrepati, Sushil Awal, Jose Oberholzer, Kathrin Maedler, Amin Ardestani
Loss of functional pancreatic β cells is a hallmark of both type 1 and 2 diabetes. Identifying the pathways that promote β cell proliferation and/or block β cell apoptosis is a potential strategy for diabetes therapy. The transcriptional coactivator Yes-associated protein (YAP), a major downstream effector of the Hippo signaling pathway, is a key regulator of organ size and tissue homeostasis by modulating cell proliferation and apoptosis. YAP is not expressed in mature primary human and mouse β cells. We aimed to identify whether reexpression of a constitutively active form of YAP promotes β cell proliferation/survival...
November 3, 2016: JCI Insight
https://www.readbyqxmd.com/read/27798063/diversity-of-species-and-antimicrobial-resistance-determinants-of-staphylococci-in-superficial-waters-in-spain
#15
Paula Gómez, Cristina Casado, Yolanda Sáenz, Laura Ruiz-Ripa, Vanesa Estepa, Myriam Zarazaga, Carmen Torres
The objectives were to determine the presence and diversity of staphylococcal species in surface waters in La Rioja region (Spain), and to characterize recovered isolates. Staphylococci were detected in 42 of 47 evaluable samples, and 72 isolates were obtained, of which 13 were coagulase-positive (CoPS) and 59 were coagulase-negative (CoNS). Twelve CoPS were identified as S. aureus and typed as follows (number of strains): t002/t502/ST5 (four), t10668/ST425 (one), t10712//ST1643 (one), t843/ST130 (one), t10855/ST2461 (one), t3369/ST2657 (one), t1166/ST133 (one), t8083/ST2049 (one) and t045/ST2460 (one); and one as S...
January 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/27783982/the-response-of-aminopeptidases-of-phaseolus-vulgaris-to-drought-depends-on-the-developmental-stage-of-the-leaves
#16
Maruška Budič, Blaž Cigić, Maja Šoštarič, Jerica Sabotič, Vladimir Meglič, Janko Kos, Marjetka Kidrič
Aminopeptidases, together with other proteases, execute and regulate the total and specifically limited protein breakdown involved in plant physiology, raising the possibility of their involvement in response to drought. We have identified, in leaves of Phaseolus vulgaris L., five aminopeptidases (E.C.3.4.11) whose levels of activity changed when three week old plants were subjected to drought. First, second and third trifoliate leaves were investigated separately. The aminopeptidases were first identified then isolated using ion exchange chromatography of leaf extracts...
December 2016: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/27760017/phenotypical-and-genotypical-antimicrobial-resistance-of-coagulase-negative-staphylococci-isolated-from-cow-mastitis
#17
I Klimiene, M Virgailis, A Pavilonis, R Siugzdiniene, R Mockeliunas, M Ruzauskas
The objectives of this study were to determine the prevalence and antimicrobial resistance of coagulase-negative staphylococci (CNS) isolated from dairy cows with subclinical mastitis. Antimicrobial resistance in staphylococci were evaluated by breakpoint values specific to the species (EU-CAST). The presence of resistance-encoding genes was detected by multiplex PCR. A total of 191 CNS isolates were obtained. The CNS isolates were typically resistant to penicillin (67.4%), tetracyc-line (18.9%), and erythromycin (13...
September 1, 2016: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/27754770/molecular-and-phenotypic-characterization-revealed-high-prevalence-of-multidrug-resistant-methicillin-susceptible-staphylococcus-aureus-in-chongqing-southwestern-china
#18
Yancheng Yang, Zhen Hu, Weilong Shang, Qiwen Hu, Junmin Zhu, Jie Yang, Huagang Peng, Xiaopeng Zhang, Hui Liu, Yanguang Cong, Shu Li, Xiaomei Hu, Renjie Zhou, Xiancai Rao
Methicillin-susceptible Staphylococcus aureus (MSSA) accounts for ∼40% of staphylococcal infections in China. However, the molecular characterization of MSSA is not well described. In this study, 124 MSSA strains collected in 2013 from a comprehensive teaching hospital in Chongqing, Southwestern China, were subjected to antibiotics susceptibility testing and molecular typing, including multilocus sequence typing, staphylococcal protein A (spa) gene typing, accessory gene regulator (agr) typing, pulsed-field gel electrophoresis (PFGE) typing, Panton-Valentine leukocidin (pvl) gene detection, and antibiotic-resistant gene detection...
October 18, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27744178/computational-identification-of-non-synonymous-polymorphisms-within-regions-corresponding-to-protein-interaction-sites
#19
Blaz Skrlj, Tanja Kunej
BACKGROUND: Protein-protein interactions (PPI) play an important role in function of all organisms and enable understanding of underlying metabolic processes. Computational predictions of PPIs are an important aspect in proteomics, as experimental methods may result in high degree of false positive results and are more expensive. Although there are many databases collecting predicted PPIs, exploration of genetics information underlying PPI interactions has not been investigated thoroughly...
October 4, 2016: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/27714592/characteristics-of-cefazolin-inoculum-effect-positive-methicillin-susceptible-staphylococcus-aureus-infection-in-a-multicentre-bacteraemia-cohort
#20
K-H Song, S-I Jung, S Lee, S Park, S M Kiem, S H Lee, Y G Kwak, Y K Kim, H-C Jang, Y-S Kim, H-I Kim, C J Kim, K-H Park, N J Kim, M-D Oh, H B Kim
Cefazolin treatment failure has been observed in high-inoculum infections caused by methicillin-susceptible Staphylococcus aureus (MSSA) with a cefazolin inoculum effect (CIE). However, data on the characteristics and risk factors for the acquisition of CIE-positive MSSA infection are scarce. CIE positivity was measured as an MIC ≥ 16 μg/ml with a high inoculum (∼5 × 10(7) CFU/ml). The blaZ gene type was assessed through sequence analysis. The clinical characteristics and risk factors for the acquisition of CIE-positive MSSA infection were assessed...
October 6, 2016: European Journal of Clinical Microbiology & Infectious Diseases
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