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Peripheral blood film

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https://www.readbyqxmd.com/read/28077149/plasmodium-falciparum-malaria-co-infection-with-tick-borne-relapsing-fever-in-dakar
#1
Mamadou A Diallo, Baidy S Kane, Mouhamadou Ndiaye, Mouhamed Dieng, Khadim Diongue, Aida S Badiane, Mame Cheikh Seck, Daouda Ndiaye
BACKGROUND: West African tick-borne relapsing fever (TBRF) due to Borrelia crocidurae and malaria are co-endemics in Senegal. Although expected to be high, co-infections are rarely reported. A case of falciparum malaria and B. crocidurae co-infection in a patient from Velingara (South of Senegal) is discussed. CASE: A 28 year-old-male patient presented to Aristide Le Dantec Hospital for recurrent fever. He initially presented to a local post health of Pikine (sub-urban of Dakar) and was diagnosed for malaria on the basis of positive malaria rapid diagnostic test (RDT) specific to Plamodium falciparum...
January 11, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28068960/nutritional-status-among-primary-school-children-in-rural-sri-lanka-a-public-health-challenge-for-a-country-with-high-child-health-standards
#2
N P G C R Naotunna, M Dayarathna, H Maheshi, G S Amarasinghe, V S Kithmini, M Rathnayaka, L Premachandra, N Premarathna, P C Rajasinghe, G Wijewardana, T C Agampodi, S B Agampodi
BACKGROUND: Nutritional status of pre adolescent children is not widely studied in Sri Lanka. The purpose of this study was to determine the nutritional status among pre-adolescent school children in a rural province of Sri Lanka. METHODS: A school based cross sectional study was carried out in North Central Province in 100 rural schools, selected using multi stage cluster sampling with probability proportionate to size. Children in grade one to five were enrolled with a maximum cluster size of fifty...
January 10, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28042208/utility-of-peripheral-film-findings-and-its-correlation-with-automated-analyzer-an-audit-from-tertiary-care-hospital
#3
Sidra Asad, Imran Ahmed, Natasha Ali
BACKGROUND AND OBJECTIVE: With the advent of automated hematology analyzer, the use of traditional microscopy of blood film has become limited. The objective of our study was to determine the percentage of peripheral blood smear review in our institution in the era of automation and to identify reasons of manual review. MATERIALS AND METHODS: This was a prospective audit from January 1, 2015, to January 15, 2015. Consecutive complete blood count (CBC) samples and peripheral smear requests made up the sample size...
January 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/27974955/reproducibility-of-manual-platelet-estimation-following-automated-low-platelet-counts
#4
Zainab S Al-Hosni, Murtadha Al-Khabori, Sahimah Al-Mamari, Jamal Al-Qasabi, Hiedi Davis, Hatim Al-Lawati, Arwa Z Al-Riyami
OBJECTIVES: Manual platelet estimation is one of the methods used when automated platelet estimates are very low. However, the reproducibility of manual platelet estimation has not been adequately studied. We sought to assess the reproducibility of manual platelet estimation following automated low platelet counts and to evaluate the impact of the level of experience of the person counting on the reproducibility of manual platelet estimates. METHODS: In this cross-sectional study, peripheral blood films of patients with platelet counts less than 100 × 10(9)/L were retrieved and given to four raters to perform manual platelet estimation independently using a predefined method (average of platelet counts in 10 fields using 100× objective multiplied by 20)...
November 2016: Oman Medical Journal
https://www.readbyqxmd.com/read/27938703/detection-and-prevalence-of-boid-inclusion-body-disease-in-collections-of-boas-and-pythons-using-immunological-assays
#5
L Chang, D Fu, M D Stenglein, J A Hernandez, J L DeRisi, E R Jacobson
Inclusion body disease (IBD) of boas and pythons is characterized by the intracytoplasmic accumulation of an antigenic 68 kDa viral protein IBDP, more recently known as the nucleoprotein (NP) of the reptarenaviruses. Blood samples of 131 captive boas and pythons (53 boa constrictors, Boa constrictor; 35 rainbow boas, Epicrates cenchria; 22 ball pythons, Python regius; 5 carpet pythons, Morelia spilota; 6 Burmese pythons, Python bivittatus; 4 Jamaican boas, Epicrates subflavus; 5 anacondas, Eunectes spp.; and 1 green tree python, Morelia viridis) were obtained from 28 collections in the USA...
December 2016: Veterinary Journal
https://www.readbyqxmd.com/read/27826893/comparison-of-time-domain-spectral-domain-and-swept-source-oct-in-evaluating-aqueous-cells-in-vitro
#6
Meng Li, Huaizhou Wang, Yue Liu, Xifang Zhang, Ningli Wang
Different optical coherence tomography (OCT), both time-domain OCT and spectral-domain OCT, has been used to evaluate anterior chamber cells in previous studies. Recently, commercial swept-source OCT was available. The comparison among three kinds of OCTs have not been done before. Here, we compared their feasibility in discerning aqueous cells in vitro. The peripheral blood was diluted to eight different cell concentrations and was packed into plastic-film bag. Each sample was tested using different scan modes of three OCTs...
December 2016: Science China. Life Sciences
https://www.readbyqxmd.com/read/27788243/submicroscopic-and-asymptomatic-plasmodium-parasitaemia-associated-with-significant-risk-of-anaemia-in-papua-indonesia
#7
Zuleima Pava, Faustina H Burdam, Irene Handayuni, Leily Trianty, Retno A S Utami, Yusrifar Kharisma Tirta, Enny Kenangalem, Daniel Lampah, Andreas Kusuma, Grennady Wirjanata, Steven Kho, Julie A Simpson, Sarah Auburn, Nicholas M Douglas, Rintis Noviyanti, Nicholas M Anstey, Jeanne R Poespoprodjo, Jutta Marfurt, Ric N Price
Submicroscopic Plasmodium infections are an important parasite reservoir, but their clinical relevance is poorly defined. A cross-sectional household survey was conducted in southern Papua, Indonesia, using cluster random sampling. Data were recorded using a standardized questionnaire. Blood samples were collected for haemoglobin measurement. Plasmodium parasitaemia was determined by blood film microscopy and PCR. Between April and July 2013, 800 households and 2,830 individuals were surveyed. Peripheral parasitaemia was detected in 37...
2016: PloS One
https://www.readbyqxmd.com/read/27630383/the-relationship-between-malaria-parasitemia-malaria-preventive-measures-and-average-birth-weight-of-babies-in-a-tertiary-facility-in-owerri-nigeria
#8
E E Idih, B U Ezem, E A Nzeribe, A O Onyegbule, B C Duru, C C Amajoyi
BACKGROUND: Despite the global efforts made to eradicate malaria, it continues to be a significant cause of morbidity and mortality in both neonates and the parturients. This study was done to determine the relationship between placental parasitemia, average neonatal birth weight and the relationship between the use of malaria preventive measures and the occurrence of placental parasitemia with the aim to improving maternal and neonatal outcome. PATIENTS AND METHODS: This cross-sectional study was done at the labor ward unit of the Federal Medical Center, Owerri, from December 2013 to May 2014...
July 2016: Nigerian Medical Journal: Journal of the Nigeria Medical Association
https://www.readbyqxmd.com/read/27609159/delayed-onset-of-congenital-hereditary-endothelial-dystrophy-due-to-compound-heterozygous-slc4a11-mutations
#9
Babu Lal Kumawat, Ranjan Gupta, Arundhati Sharma, Seema Sen, Shikha Gupta, Radhika Tandon
BACKGROUND: Congenital hereditary endothelial dystrophy (CHED) is an autosomal recessive disorder characterized by bilateral, symmetrical, noninflammatory corneal clouding (edema) present at birth or shortly thereafter. This study reports on an unusual delayed presentation of CHED with compound heterozygous SLC4A11 mutations. MATERIALS AND METHODS: A 45-year-old female, presenting with bilateral decreased vision since childhood that deteriorated in the last 5 years, was evaluated to rule out trauma, viral illness, chemical injury, glaucoma, and corneal endothelial dystrophies...
July 2016: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/27554598/short-term-culture-of-monocytes-as-an-in%C3%A2-vitro-evaluation-system-for-bionanomaterials-designated-for-medical-use
#10
Ekaterina Igorevna Shishatskaya, Dragana Nikitovic, Alexander Shabanov Vasilievich, George N Tzanakakis, Aristidis M Tsatsakis, Natalia Gennadievna Menzianova
We studied the feasibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bionanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro- and nano-dimensions, fullerenes (F) and nanodiamonds (ND), which are either currently in use and/or potentially applicable in medicine. Additionally, the effect of creating a protein corona on ND and F particles was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a well-established tool for assessing the viability/metabolic activity of cells...
October 2016: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/27462588/a-successful-treatment-of-hypercalcemia-with-zoledronic-acid-in-a-15-year-old-boy-with-acute-lymphoblastic-leukemia
#11
Hye-Jin Park, Eun-Jin Choi, Jin-Kyung Kim
Severe hypercalcemia in children is a rare medical emergency. We present a case of a 15-year-old boy with hypercalcemia (total calcium level, 14.2 mg/dL) with a normal complete blood count, no circulating blasts in the peripheral blood film, and no other signs of acute lymphoblastic leukemia (ALL), including no signs of lymphadenopathy or hepatosplenomegaly. The hypercalcemia was successfully treated with zoledronic acid. As hypercalcemia can be the only presenting symptom of ALL in children, the diagnosis is often delayed...
June 2016: Annals of Pediatric Endocrinology & Metabolism
https://www.readbyqxmd.com/read/27427750/nanoparticles-with-therapeutic-properties-generate-various-response-of-human-peripheral-blood-mononuclear-cells
#12
Marzena Szwed, Ralph Santos-Oliveira
In the present study we report the interactions of four types of different nanoparticles with normal peripheral blood mononuclear cells. To our research we chose four types of nanoparticles which possess therapeutic properties (Trastuzumab, ethylene-diamine-tetra-methylene-phosphonic for breast and bone cancers treatment, respectively) or can be used as the ingredients of sun-protected films (nanoemulsions with or without chitosan). By carrying out XTT survival assay we observed that both types of tested nanoemulsions suppressed the proliferation of normal lymphocytes...
June 2016: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/27424614/a-comparative-study-of-azithromycin-and-sulphadoxine-pyrimethamine-as-prophylaxis-against-malaria-in-pregnancy
#13
Rukiyat Adeola Abdus-Salam, Folasade Adenike Bello, Fatai A Fehintola, Ayodele O Arowojolu
CONTEXT: The benefit of malaria prophylaxis in pregnancy is threatened by emergence of Plasmodium falciparum resistance to antimalarial agents for chemoprophylaxis and treatment. AIM: This study aimed to compare the effectiveness of azithromycin (AZ) with sulphadoxine-pyrimethamine (SP) for malaria prevention. SETTINGS AND DESIGN: A prospective comparative study of antenatal clinic attendees at the University College Hospital, Ibadan, Nigeria...
April 2016: Nigerian Postgraduate Medical Journal
https://www.readbyqxmd.com/read/27413326/haemato-biochemical-and-thyroxin-status-in-trypanosoma-evansi-infected-dogs
#14
B Sudhakara Reddy, K Nalini Kumari, S Sivajothi, V C Rayulu
In one year period of study, dogs with inappetence, fever, ocular discharges, dullness, enlarged lymph nodes were screened for the presence of haemoprotozoans at College Hospital of College of Veterinary Science, Tirupati. Wet blood film examination and stained blood smear examination was done to confirm the condition. Peripheral blood smears of dogs revealed the presence of Trypansomes. Trypanosoma evansi was confirmed based on the morphology and measurements of the organisms in the stained blood smears.  Haematology revealed decreased total erythrocyte count, packed cell volume, haemoglobin and total leucocyte count values...
June 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27380711/elevated-monocyte-phosphorylated-p38-in-nearby-employees-after-a-chemical-explosion
#15
André Sulen, Stein H L Lygre, Sigrun M Hjelle, Bjørg E Hollund, Bjørn T Gjertsen
Personalised health surveillance is infrequent or absent in occupational and environmental medicine. The shortage of functional tests in relevant cells and tissues greatly limits our understanding of environmental exposures and associated disease risk. We evaluated single cell signalling in peripheral blood mononuclear cells from 301 individuals in a cross sectional health survey 18 months after a chemical explosion of sulphorous coker gasoline. The accident created a malodourous environment leading to long-term health complaints...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27377175/semi-automatic-rating-method-for-neutrophil-alkaline-phosphatase-activity
#16
Kanae Sugano, Kotomi Hashi, Misaki Goto, Kiyotaka Nishi, Rie Maeda, Keigo Kono, Mai Yamamoto, Kazunori Okada, Sanae Kaga, Keiko Miwa, Taisei Mikami, Nobuo Masauzi
BACKGROUND: The neutrophil alkaline phosphatase (NAP) score is a valuable test for the diagnosis of myeloproliferative neoplasms, but it has still manually rated. Therefore, we developed a semi-automatic rating method using Photoshop(®) and Image-J, called NAP-PS-IJ. METHODS: Neutrophil alkaline phosphatase staining was conducted with Tomonaga's method to films of peripheral blood taken from three healthy volunteers. At least 30 neutrophils with NAP scores from 0 to 5+ were observed and taken their images...
July 4, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27315799/malaria-prevention-practices-and-delivery-outcome-a-cross-sectional-study-of-pregnant-women-attending-a-tertiary-hospital-in-northeastern-nigeria
#17
Hamzat U Muhammad, Fatima J Giwa, Adebola T Olayinka, Shakir M Balogun, IkeOluwapo Ajayi, Olufemi Ajumobi, Patrick Nguku
BACKGROUND: Malaria in pregnancy remains a public health problem in Nigeria. It causes maternal anaemia and adversely affects birth outcome leading to low birth weight, abortions and still births. Nigeria has made great strides in addressing the prevention and control of malaria in pregnancy. However, recent demographic survey shows wide disparities in malaria control activities across the geopolitical zones. This situation has been compounded by the political unrest and population displacement especially in the Northeastern zone leaving a significant proportion of pregnant women at risk of diseases, including malaria...
2016: Malaria Journal
https://www.readbyqxmd.com/read/27299745/pain-related-reactions-among-premature-infants-with-gestational-age-less-than-26-weeks-an-observational-cohort-study
#18
Kyriakos Martakis, Christoph Hünseler, Kruthika Thangavelu, Angela Kribs, Bernhard Roth
INTRODUCTION: There is insufficient information regarding acute pain reactions among premature infants with a gestational age of less than 26 weeks and no appropriate scale for pain measurement in this age group. We hypothesized that these infants present specific reactions to a standardized pain stimulus within the first 3 days of life. METHODOLOGY: Mixed-methods, prospective, open-label, single-arm, observational study. Routine capillary or peripheral blood takes were filmed...
2016: Neonatology
https://www.readbyqxmd.com/read/27277355/a-survey-on-current-practice-of-management-of-early-onset-neonatal-sepsis
#19
A C Dey, M I Hossain, S Afroze, S K Dey, M A Mannan, M Shahidullah
It was a survey type of cross sectional study where the participants were from different teaching/referral hospital across the country and was done to gather information regarding current practice of management of neonatal sepsis among paediatricians and neonatologists and was conducted on the spot during a national conference of Bangladesh Perinatal Society in December 2013. Specialists in neonatology, paediatrics, and some other disciplines working in different institutes across the country were requested to respond...
April 2016: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/27269821/mimicking-form-and-function-of-native-small-diameter-vascular-conduits-using-mulberry-and-non-mulberry-patterned-silk-films
#20
Prerak Gupta, Manishekhar Kumar, Nandana Bhardwaj, Jadi Praveen Kumar, C S Krishnamurthy, Samit Kumar Nandi, Biman B Mandal
Autologous graft replacement as a strategy to treat diseased peripheral small diameter (≤6 mm) blood vessel is often challenged by prior vein harvesting. To address this issue, we fabricated native-tissue mimicking multilayered small diameter vascular graft (SDVG) using mulberry (Bombyx mori) and Indian endemic non-mulberry (Antheraea assama and Philosamia ricini) silk. Patterned silk films were fabricated on microgrooved PDMS mold, casted by soft lithography. The biodegradable patterned film templates with aligned cell sheets were rolled onto an inert mandrel to mimic vascular conduit...
June 29, 2016: ACS Applied Materials & Interfaces
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