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Peripheral blood film

Meng Li, Huaizhou Wang, Yue Liu, Xifang Zhang, Ningli Wang
Different optical coherence tomography (OCT), both time-domain OCT and spectral-domain OCT, has been used to evaluate anterior chamber cells in previous studies. Recently, commercial swept-source OCT was available. The comparison among three kinds of OCTs have not been done before. Here, we compared their feasibility in discerning aqueous cells in vitro. The peripheral blood was diluted to eight different cell concentrations and was packed into plastic-film bag. Each sample was tested using different scan modes of three OCTs...
November 4, 2016: Science China. Life Sciences
Zuleima Pava, Faustina H Burdam, Irene Handayuni, Leily Trianty, Retno A S Utami, Yusrifar Kharisma Tirta, Enny Kenangalem, Daniel Lampah, Andreas Kusuma, Grennady Wirjanata, Steven Kho, Julie A Simpson, Sarah Auburn, Nicholas M Douglas, Rintis Noviyanti, Nicholas M Anstey, Jeanne R Poespoprodjo, Jutta Marfurt, Ric N Price
Submicroscopic Plasmodium infections are an important parasite reservoir, but their clinical relevance is poorly defined. A cross-sectional household survey was conducted in southern Papua, Indonesia, using cluster random sampling. Data were recorded using a standardized questionnaire. Blood samples were collected for haemoglobin measurement. Plasmodium parasitaemia was determined by blood film microscopy and PCR. Between April and July 2013, 800 households and 2,830 individuals were surveyed. Peripheral parasitaemia was detected in 37...
2016: PloS One
E E Idih, B U Ezem, E A Nzeribe, A O Onyegbule, B C Duru, C C Amajoyi
BACKGROUND: Despite the global efforts made to eradicate malaria, it continues to be a significant cause of morbidity and mortality in both neonates and the parturients. This study was done to determine the relationship between placental parasitemia, average neonatal birth weight and the relationship between the use of malaria preventive measures and the occurrence of placental parasitemia with the aim to improving maternal and neonatal outcome. PATIENTS AND METHODS: This cross-sectional study was done at the labor ward unit of the Federal Medical Center, Owerri, from December 2013 to May 2014...
July 2016: Nigerian Medical Journal: Journal of the Nigeria Medical Association
Babu Lal Kumawat, Ranjan Gupta, Arundhati Sharma, Seema Sen, Shikha Gupta, Radhika Tandon
BACKGROUND: Congenital hereditary endothelial dystrophy (CHED) is an autosomal recessive disorder characterized by bilateral, symmetrical, noninflammatory corneal clouding (edema) present at birth or shortly thereafter. This study reports on an unusual delayed presentation of CHED with compound heterozygous SLC4A11 mutations. MATERIALS AND METHODS: A 45-year-old female, presenting with bilateral decreased vision since childhood that deteriorated in the last 5 years, was evaluated to rule out trauma, viral illness, chemical injury, glaucoma, and corneal endothelial dystrophies...
July 2016: Indian Journal of Ophthalmology
Ekaterina Igorevna Shishatskaya, Dragana Nikitovic, Alexander Shabanov Vasilievich, George N Tzanakakis, Aristidis M Tsatsakis, Natalia Gennadievna Menzianova
We studied the feasibility of using a short-term culture of monocytes, isolated from peripheral donor blood, to assess the biological activity of different types of bionanomaterials (BNM): biodegradable polimeric particles, fiber and film substrates of micro- and nano-dimensions, fullerenes (F) and nanodiamonds (ND), which are either currently in use and/or potentially applicable in medicine. Additionally, the effect of creating a protein corona on ND and F particles was investigated. The cellular reduction of (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a well-established tool for assessing the viability/metabolic activity of cells...
October 2016: Food and Chemical Toxicology
Hye-Jin Park, Eun-Jin Choi, Jin-Kyung Kim
Severe hypercalcemia in children is a rare medical emergency. We present a case of a 15-year-old boy with hypercalcemia (total calcium level, 14.2 mg/dL) with a normal complete blood count, no circulating blasts in the peripheral blood film, and no other signs of acute lymphoblastic leukemia (ALL), including no signs of lymphadenopathy or hepatosplenomegaly. The hypercalcemia was successfully treated with zoledronic acid. As hypercalcemia can be the only presenting symptom of ALL in children, the diagnosis is often delayed...
June 2016: Annals of Pediatric Endocrinology & Metabolism
Marzena Szwed, Ralph Santos-Oliveira
In the present study we report the interactions of four types of different nanoparticles with normal peripheral blood mononuclear cells. To our research we chose four types of nanoparticles which possess therapeutic properties (Trastuzumab, ethylene-diamine-tetra-methylene-phosphonic for breast and bone cancers treatment, respectively) or can be used as the ingredients of sun-protected films (nanoemulsions with or without chitosan). By carrying out XTT survival assay we observed that both types of tested nanoemulsions suppressed the proliferation of normal lymphocytes...
June 2016: Journal of Nanoscience and Nanotechnology
Rukiyat Adeola Abdus-Salam, Folasade Adenike Bello, Fatai A Fehintola, Ayodele O Arowojolu
CONTEXT: The benefit of malaria prophylaxis in pregnancy is threatened by emergence of Plasmodium falciparum resistance to antimalarial agents for chemoprophylaxis and treatment. AIM: This study aimed to compare the effectiveness of azithromycin (AZ) with sulphadoxine-pyrimethamine (SP) for malaria prevention. SETTINGS AND DESIGN: A prospective comparative study of antenatal clinic attendees at the University College Hospital, Ibadan, Nigeria...
April 2016: Nigerian Postgraduate Medical Journal
B Sudhakara Reddy, K Nalini Kumari, S Sivajothi, V C Rayulu
In one year period of study, dogs with inappetence, fever, ocular discharges, dullness, enlarged lymph nodes were screened for the presence of haemoprotozoans at College Hospital of College of Veterinary Science, Tirupati. Wet blood film examination and stained blood smear examination was done to confirm the condition. Peripheral blood smears of dogs revealed the presence of Trypansomes. Trypanosoma evansi was confirmed based on the morphology and measurements of the organisms in the stained blood smears.  Haematology revealed decreased total erythrocyte count, packed cell volume, haemoglobin and total leucocyte count values...
June 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
André Sulen, Stein H L Lygre, Sigrun M Hjelle, Bjørg E Hollund, Bjørn T Gjertsen
Personalised health surveillance is infrequent or absent in occupational and environmental medicine. The shortage of functional tests in relevant cells and tissues greatly limits our understanding of environmental exposures and associated disease risk. We evaluated single cell signalling in peripheral blood mononuclear cells from 301 individuals in a cross sectional health survey 18 months after a chemical explosion of sulphorous coker gasoline. The accident created a malodourous environment leading to long-term health complaints...
2016: Scientific Reports
Kanae Sugano, Kotomi Hashi, Misaki Goto, Kiyotaka Nishi, Rie Maeda, Keigo Kono, Mai Yamamoto, Kazunori Okada, Sanae Kaga, Keiko Miwa, Taisei Mikami, Nobuo Masauzi
BACKGROUND: The neutrophil alkaline phosphatase (NAP) score is a valuable test for the diagnosis of myeloproliferative neoplasms, but it has still manually rated. Therefore, we developed a semi-automatic rating method using Photoshop(®) and Image-J, called NAP-PS-IJ. METHODS: Neutrophil alkaline phosphatase staining was conducted with Tomonaga's method to films of peripheral blood taken from three healthy volunteers. At least 30 neutrophils with NAP scores from 0 to 5+ were observed and taken their images...
July 4, 2016: Journal of Clinical Laboratory Analysis
Hamzat U Muhammad, Fatima J Giwa, Adebola T Olayinka, Shakir M Balogun, IkeOluwapo Ajayi, Olufemi Ajumobi, Patrick Nguku
BACKGROUND: Malaria in pregnancy remains a public health problem in Nigeria. It causes maternal anaemia and adversely affects birth outcome leading to low birth weight, abortions and still births. Nigeria has made great strides in addressing the prevention and control of malaria in pregnancy. However, recent demographic survey shows wide disparities in malaria control activities across the geopolitical zones. This situation has been compounded by the political unrest and population displacement especially in the Northeastern zone leaving a significant proportion of pregnant women at risk of diseases, including malaria...
2016: Malaria Journal
Kyriakos Martakis, Christoph Hünseler, Kruthika Thangavelu, Angela Kribs, Bernhard Roth
INTRODUCTION: There is insufficient information regarding acute pain reactions among premature infants with a gestational age of less than 26 weeks and no appropriate scale for pain measurement in this age group. We hypothesized that these infants present specific reactions to a standardized pain stimulus within the first 3 days of life. METHODOLOGY: Mixed-methods, prospective, open-label, single-arm, observational study. Routine capillary or peripheral blood takes were filmed...
2016: Neonatology
A C Dey, M I Hossain, S Afroze, S K Dey, M A Mannan, M Shahidullah
It was a survey type of cross sectional study where the participants were from different teaching/referral hospital across the country and was done to gather information regarding current practice of management of neonatal sepsis among paediatricians and neonatologists and was conducted on the spot during a national conference of Bangladesh Perinatal Society in December 2013. Specialists in neonatology, paediatrics, and some other disciplines working in different institutes across the country were requested to respond...
April 2016: Mymensingh Medical Journal: MMJ
Prerak Gupta, Manishekhar Kumar, Nandana Bhardwaj, Jadi Praveen Kumar, C S Krishnamurthy, Samit Kumar Nandi, Biman B Mandal
Autologous graft replacement as a strategy to treat diseased peripheral small diameter (≤6 mm) blood vessel is often challenged by prior vein harvesting. To address this issue, we fabricated native-tissue mimicking multilayered small diameter vascular graft (SDVG) using mulberry (Bombyx mori) and Indian endemic non-mulberry (Antheraea assama and Philosamia ricini) silk. Patterned silk films were fabricated on microgrooved PDMS mold, casted by soft lithography. The biodegradable patterned film templates with aligned cell sheets were rolled onto an inert mandrel to mimic vascular conduit...
June 29, 2016: ACS Applied Materials & Interfaces
Lucia Fontanelli Sulekova, Simona Gabrielli, Maurizio De Angelis, Giovanni L Milardi, Carlo Magnani, Biancamaria Di Marco, Gloria Taliani, Gabriella Cancrini
BACKGROUND: Human dirofilariosis is still a little known infection even in endemic areas. Dirofilariosis is zoonotic infection usually abortive in humans; instead, we report a very rare case (the 4th in the world), the first in Italy, in which at least two infective larvae became mature adults that mated and produced active microfilariae even though they did not reach peripheral blood. CASE PRESENTATION: A 30-year-old Italian woman presented with a transient oedematous swelling on the left abdominal wall with a creeping eruption followed by the occurrence of a subcutaneous nodular painless mass in the iliac region...
2016: BMC Infectious Diseases
Samuel Wanji, Dizzle Bita Tayong, Laura E Layland, Fabrice R Datchoua Poutcheu, Winston Patrick Chounna Ndongmo, Jonas Arnaud Kengne-Ouafo, Manuel Ritter, Nathalie Amvongo-Adjia, Fanny Fri Fombad, Charity Nya Njeshi, Armand Seraphin Nkwescheu, Peter A Enyong, Achim Hoerauf
BACKGROUND: Mansonellosis remains one of the most neglected of tropical diseases and its current distribution in the entire forest block of southern Cameroon is unknown. In order to address this issue, we have surveyed the distribution of Mansonella perstans in different bioecological zones and in addition, elucidated the influence of multiple rounds of ivermectin (IVM) based mass drug administration (MDA). METHODS: A mixed design was used. Between 2000 and 2014, both cross-sectional and longitudinal surveys were carried out in 137 communities selected from 12 health districts belonging to five main bioecological zones of the southern part of Cameroon...
2016: Parasites & Vectors
Chih-Chun Chang, Jen-Tang Sun, Tse-Hsuan Liou, Chin-Fu Kuo, Chia-Hao Bei, Sheng-Jun Lin, Wei-Ting Tsai, N-Chi Tan, Ching-Biau Liou, Ming-Jang Su, Tzung-Hai Yen, Fang-Yeh Chu
BACKGROUND: It is reported that the percentage of smudge cells in the blood smear could be a prognostic indicator in chronic lymphocytic leukemia. However, the clinical significance of smudge cells in other hematological malignancies, solid tumors or non-malignant diseases is less clear. Hence, this study was conducted to survey the clinical significance of smudge cells in hematological cancers and other disorders. MATERIALS AND METHODS: From January to November, 2015, the clinical data of patients who received blood examination with differential counts for clinical purpose and were found to have smudge cells in the peripheral blood film in Far Eastern Memorial Hospital were selected...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
SreyRam Kuy, Ramon A L Romero, SreyReath Kuy
A 67-year old man presented with a painful left foot and a putrid odor. His past medical history was significant for poorly controlled diabetes mellitus, coronary artery disease, and peripheral vascular disease. His surgical history included a prior right below-knee amputation for a diabetic foot infection three years prior, and a left third toe amputation for osteomyelitis one month ago. He was an active smoker. His laboratory data revealed a white blood count of 22 k/uL and a blood glucose of 381 mg/dL. Physical exam demonstrated an erythematous and edematous left foot with subcutaneous crepitus along the plantar surface...
September 2015: Journal of the Louisiana State Medical Society: Official Organ of the Louisiana State Medical Society
María Luisa Lozano, N Revilla, T J Gonzalez-Lopez, S Novelli, J R González-Porras, B Sánchez-Gonzalez, N Bermejo, S Pérez, F J Lucas, M T Álvarez, M J Arilla, M Perera, J do Nascimento, R M Campos, L F Casado, V Vicente
Very few data exist on the management of adult patients diagnosed with primary immune thrombocytopenia (ITP). The objectives of this study were to describe the diagnostic and treatment patterns for ITP and to compare the findings to recent ITP guidelines. We retrospectively analyzed the medical records of adult ITP patients diagnosed with primary ITP between January 2011 and June 2012 and examined whether management strategies were consistent or not with eight recent guideline-recommended practices. Overall, median age at the diagnosis of the disease (n = 101) was 58 years and median platelet count 12 × 10(9)/L with 75...
June 2016: Annals of Hematology
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