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Peripheral blood film

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https://www.readbyqxmd.com/read/28715687/highly-selective-and-sensitive-fluorogenic-ferric-probes-based-on-aggregation-enhanced-emission-with-sime3-substituted-polybenzene
#1
Xuefeng Wang, Hua Wang, Qin Jiang, Yong-Ill Lee, Shengyu Feng, Hong-Guo Liu
In this study, thiophene was linked to polybenzene to generate novel fluorescent probes, namely 3,4-diphenyl-2,5-di(2-thienyl)phenyl-trimethylsilane (DPTB-TMS) with a -SiMe3 substituent and 3,4-diphenyl-2,5-di(2-thienyl)phenyl (DPTB) without the -SiMe3 substituent, respectively. Both of the two compounds exhibit aggregation-enhanced emission (AEE) properties in tetrahydrofuran/water mixtures due to restricted intramolecular rotation of the peripheral groups, which make the two compounds good candidates for the detection of Fe(3+) ions in aqueous-based solutions...
July 13, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28653041/plasmodium-falciparum-genotypes-in-matched-peripheral-placental-and-umbilical-cord-blood-in-an-area-characterised-by-unstable-malaria-transmission-in-eastern-sudan
#2
Omar E Fadlelseed, Maha E Osman, Nahla M Shamseldin, Amar B Elhussein, Ishag Adam
BACKGROUND: There are few published studies on Plasmodium falciparum genotypes in peripheral, placental and umbilical cord blood in areas characterised by unstable malaria transmission. METHOD: A cross-sectional study was conducted to investigate P. falciparum genotypes in matched peripheral, placental and umbilical cord blood in eastern Sudan. Thick blood smears and P. falciparum merozoite surface protein 1 (MSP1) and 2 (MSP2) genes as polymorphic markers in polymerase chain reactions were investigated in 3 kinds of samples of 153 pregnant women at delivery...
June 2017: Heliyon
https://www.readbyqxmd.com/read/28644307/x-linked-sideroblastic-anemia-in-a-malay-boy-with-alas2-s568g-mutation
#3
Thomas A K Susanto, Rajat Bhattacharyya
Dimorphism in peripheral blood film was noted in a 16 year old Malay boy with anemia who was eventually diagnosed with X-linked sideroblastic anemia. A mutation in ALAS2 S568G was identified which has not been described previously in a Malay ethnic group.
July 2017: Journal of Pediatric Hematology/oncology
https://www.readbyqxmd.com/read/28594466/bleeding-is-not-the-main-clinical-issue-in-many-patients-with-inherited-thrombocytopaenias
#4
REVIEW
F Melazzini, C Zaninetti, C L Balduini
Bleeding diathesis has been considered for a long time the main clinical issue impacting the lives of patients affected by inherited thrombocytopaenias. However, the number of known inherited thrombocytopaenias greatly increased in recent years, and careful evaluation of hundreds of patients affected by these 'new' disorders revealed that most of them are at risk of developing additional life-threatening disorders during childhood or adult life. These additional disorders are usually more serious and dangerous than low platelet count...
June 8, 2017: Haemophilia: the Official Journal of the World Federation of Hemophilia
https://www.readbyqxmd.com/read/28576331/rapid-preparation-and-single-cell-analysis-of-concentrated-blood-smears-using-a-high-throughput-blood-cell-separator-and-a-microfabricated-grid-film
#5
Dongwon You, Sein Oh, Byeongyeon Kim, Young Ki Hahn, Sungyoung Choi
Cytological examination of peripheral white blood cells inhomogeneously distributed on a blood smear is currently limited by the low abundance and random sampling of the target cells. To address the challenges, we present a new approach to prepare and analyze concentrated blood smears by rapidly enriching white blood cells up to 32-fold with 92% recovery on average at a high throughput (1mL/min) using a deterministic migration-based separator and by systematically analyzing a large number of the cells distributed over a blood slide using a microfabricated grid film...
May 24, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28549617/hematopathological-alterations-of-major-tumor-suppressor-cascade-vital-cell-cycle-inhibitors-and-hematopoietic-niche-components-in-experimental-myelodysplasia
#6
Ritam Chatterjee, Shubhangi Gupta, Sujata Law
Myelodysplastic syndrome (MDS) is a poorly understood dreadful hematopoietic disorder that involves maturational defect and abnormalities in blood cell production leading to dysplastic changes and peripheral blood pancytopenia. The present work aims in establishing the mechanistic relationship of the expressional alterations of major tumor suppressor cascade, vital cell cycle inhibitors and hematopoietic microenvironmental components with the disease pathophysiologies. The study involves the development of N-N' Ethylnitrosourea (ENU) induced mouse model of MDS, characterization of the disease with blood film and bone marrow smear studies, scanning electron microscopic observation, mitochondrial membrane potential determination, flowcytometric analysis of osteoblastic and vascular niche components along with the expressional study of cleaved caspase-3, PCNA, Chk-2, p53, Ndn, Gfi-1, Tie-2, Sdf-1, Gsk-3β, p18 and Myt-1 in the bone marrow compartment...
May 23, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28476929/the-case-of-the-mysterious-vanishing-spleen-autosplenectomy-complicating-pneumococcal-sepsis
#7
Georgina Moritz, Megan Jenkins, Dushyant Shetty, Julie Blundell
A 57-year-old previously healthy fisherman was admitted in fulminant pneumococcal septic shock, with disseminated intravascular coagulation, requiring aggressive management including bilateral below-knee amputations for ischaemic necrosis. He began to recover and was discharged for rehabilitation, however during his convalescence was found to be hypercalcaemic. No malignancy was found on CT scan, but it was noted that his spleen was absent, replaced by a 4 cm smooth-walled, fluid-filled lesion. This was unexpected as an ultrasound in intensive care 10 weeks previously had demonstrated a normal spleen...
May 4, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28474283/hucmncs-protect-vascular-endothelium-and-prevent-isr-after-endovascular-interventional-therapy-for-vascular-diseases-in-t2dm-rabbits
#8
Haixia Ding, Tong Zhang, Yaping Du, Bei Liu, Yueqin Liu, Fujun Wang
The therapeutic effect of transplantation of human umbilical cord blood cell-derived mononuclear cells (HUCMNCs) on treating in-stent restenosis (ISR) after endovascular interventional therapy (EIT) was evaluated in preclinical rabbit model of type 2 diabetes mellitus (T2DM)-related peripheral artery disease (PAD). HUCMNCs were transplanted to T2DM rabbits subjected to femoral artery occlusion surgery and received EIT. Serum concentration of soluble vascular endothelial cadherin (VE-cad) and plasma concentration of lipoprotein-associated phospholipase A2 (Lp-PLA2) were determined with enzyme-linked immunosorbent assay before and after the transplantation...
May 4, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28399686/prevalence-of-congenital-malaria-in-newborns-of-mothers-co-infected-with-hiv-and-malaria-in-benin-city
#9
Fidelis Ewenitie Eki-Udoko, Ayebo E Sadoh, Michael O Ibadin, Augustine I Omoigberale
BACKGROUND: HIV and Plasmodium falciparum malaria co-infection annually complicates about one million pregnancies in sub-Saharan Africa. Congenital malaria (CM) has deleterious effects on newborns. Little is known about the effect of co-infections on the prevalence of CM in infants born by these women. This study was carried out to determine the prevalence of CM in newborns of mothers co-infected with HIV and malaria compared to HIV-negative mothers with malaria in Benin-City. METHODS: Subjects were 162 newborns of mothers co-infected with HIV and malaria...
April 11, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28388959/autosomal-recessive-inherited-bleeding-disorders-in-pakistan-a-cross-sectional-study-from-selected-regions
#10
Arshi Naz, Muhammad Younus Jamal, Samina Amanat, Ikram Din Ujjan, Akber Najmuddin, Humayun Patel, Fazle Raziq, Nisar Ahmed, Ayisha Imran, Tahir Sultan Shamsi
BACKGROUND: Autosomal recessive bleeding disorders (ARBDs) include deficiencies of clotting factors I, II, V, VII, X, XI, XIII, vitamin K dependent clotting factors, combined factor V & VIII, Von Willebrand Disease (vWD) type 3, Glanzmann's thrombasthenia (GT) and Bernard-Soulier syndrome. Patients with primary bleeding disorders from all the major provincial capitals of Pakistan were screened for ARBDs. Prothrombin (PT), activated partial thromboplastin time (APTT), bleeding time (BT) and fibrinogen levels were measured...
April 7, 2017: Orphanet Journal of Rare Diseases
https://www.readbyqxmd.com/read/28352150/clinical-and-allergological-analysis-of-ocular-manifestations-of-sick-building-syndrome
#11
Yusuke Saeki, Kazuaki Kadonosono, Eiichi Uchio
PURPOSE: The disease concept of sick building syndrome (SBS) is still unclear. Ocular mucous membrane irritation is one of the major symptoms of SBS. However, the immunological aspects of the ocular complications of SBS are not yet clarified. The clinical and allergological aspects of SBS cases with ocular disorders with special reference to allergic conjunctival diseases (ACD) were analyzed, especially with respect to local immunological features. METHODS: Twelve cases of SBS with ocular findings and 49 cases of ACD (allergic conjunctivitis [AC], atopic keratoconjunctivitis [AKC], and vernal keratoconjunctivitis [VKC]) for comparison were evaluated...
2017: Clinical Ophthalmology
https://www.readbyqxmd.com/read/28347295/factors-associated-with-anemia-among-sri-lankan-primary-school-children-in-rural-north-central-province
#12
Gayani Shashikala Amarasinghe, Naotunna Palliya Guruge Chamidri Randika Naottunna, Thilini Chanchala Agampodi, Suneth Buddhika Agampodi
BACKGROUND: Despite interventions, childhood anemia is still a major public health problem in low and middle income countries. Purpose of the present study is to determine factors associated with anemia among rural primary school children in Sri Lanka, a country undergoing rapid socioeconomic changes. METHODS: Multi stage cluster sampling was used to select 100 rural schools in NCP and a maximum of 50 children aged 60-131 months were enrolled from each school. Self-administered questionnaires were sent to parents...
March 27, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28345407/knee-menisci
#13
James Kevin Bryceland, Andrew John Powell, Thomas Nunn
The menisci of the knees are semicircular fibrocartilaginous structures consisting of a hydrophilic extracellular matrix containing a network of collagen fibers, glycoproteins, and proteoglycans maintained by a cellular component. The menisci are responsible for more than 50% of load transmission across the knee and increase joint congruity thereby also aiding in fluid film lubrication of the joint. In the United Kingdom, meniscal tears are the most common form of intra-articular knee injury and one of the commonest indications for orthopedic intervention...
April 2017: Cartilage
https://www.readbyqxmd.com/read/28320389/age-and-geographic-patterns-of-plasmodium-falciparum-malaria-infection-in-a-representative-sample-of-children-living-in-burkitt-lymphoma-endemic-areas-of-northern-uganda
#14
Marlena Maziarz, Tobias Kinyera, Isaac Otim, Paul Kagwa, Hadijah Nabalende, Ismail D Legason, Martin D Ogwang, Samuel Kirimunda, Benjamin Emmanuel, Steven J Reynolds, Patrick Kerchan, Moses M Joloba, Andrew W Bergen, Kishor Bhatia, Ambrose O Talisuna, Robert J Biggar, James J Goedert, Ruth M Pfeiffer, Sam M Mbulaiteye
BACKGROUND: Falciparum malaria is an important risk factor for African Burkitt lymphoma (BL), but few studies have evaluated malaria patterns in healthy BL-age children in populations where both diseases are endemic. To obtain accurate current data, patterns of asymptomatic malaria were investigated in northern Uganda, where BL is endemic. METHODS: Between 2011 and 2015, 1150 apparently healthy children under 15 years old were sampled from 100 villages in northern Uganda using a stratified, multi-stage, cluster survey design...
March 20, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28316450/a-case-of-anemia-how-important-is-peripheral-blood-film-before-transfusing
#15
Pankaj Kumar, Geetanjali Jindal, Vishal Guglani
No abstract text is available yet for this article.
January 2017: Asian Journal of Transfusion Science
https://www.readbyqxmd.com/read/28265257/regulatory-b-cells-in-seropositive-myasthenia-gravis-versus-healthy-controls
#16
Md Rezaul Karim, Hong-Yan Zhang, Jiang Yuan, Qiang Sun, Yun-Fu Wang
OBJECTIVE: To find out if the failure in immunotolerance of myasthenia gravis (MG) is a possible aspect of deduction in Breg cells and to characterize B cell subsets in MG. METHODS: Flow cytometry detection and enzyme-linked immunosorbent assays in peripheral blood films of 10 MG patients and 10 healthy controls (HCs) were performed after isolation of B cells. The CD19(+)CD5(+)CD1d(+) Breg cells percentages were measured to complement a B cell phenotype assay and frequencies of B cell subsets...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28191805/ring-chromosome-may-signal-progression-of-fanconi-anemia
#17
Ream Elzain Abdelgadir, Kulthom Mohamed, Imad Fadl Elmula
BACKGROUND: Fanconi anemia (FA) is a genomic instability disorder associated with high risk of AML. Ring chromosomes are results of genomic instability and observed in many human neoplasias. The present study aimed to assess the role of ring chromosome in the progression of FA. MATERIAL AND METHODS: The study included 60 patients with provisional diagnosis of FA. A total of 5 ml of venous blood was collected and processed for complete hemogram, peripheral blood film, and breakage test...
September 2016: Gulf Journal of Oncology
https://www.readbyqxmd.com/read/28184306/effect-of-malaria-infection-on-hematological-profiles-of-people-living-with-human-immunodeficiency-virus-in-gambella-southwest-ethiopia
#18
Tsion Sahle, Tilahun Yemane, Lealem Gedefaw
BACKGROUND: Malaria and human immunodeficiency virus are the two most devastating global health problems causing more than two million deaths each year. Hematological abnormalities such as anemia, thrombocytopenia and leucopenia are the common complications in malaria and HIV co-infected individuals. The aim of this study was to determine the effect of malaria infection on hematological profiles of people living with HIV attending Gambella Hospital ART clinic, Southwestern Ethiopia. OBJECTIVE: To determine the effect of malaria infection on hematological profiles of people living with HIV attending Gambella Hospital ART clinic, Southwestern Ethiopia...
2017: BMC Hematology
https://www.readbyqxmd.com/read/28077149/plasmodium-falciparum-malaria-co-infection-with-tick-borne-relapsing-fever-in-dakar
#19
Mamadou A Diallo, Baidy S Kane, Mouhamadou Ndiaye, Mouhamed Dieng, Khadim Diongue, Aida S Badiane, Mame Cheikh Seck, Daouda Ndiaye
BACKGROUND: West African tick-borne relapsing fever (TBRF) due to Borrelia crocidurae and malaria are co-endemics in Senegal. Although expected to be high, co-infections are rarely reported. A case of falciparum malaria and B. crocidurae co-infection in a patient from Velingara (South of Senegal) is discussed. CASE: A 28 year-old-male patient presented to Aristide Le Dantec Hospital for recurrent fever. He initially presented to a local post health of Pikine (sub-urban of Dakar) and was diagnosed for malaria on the basis of positive malaria rapid diagnostic test (RDT) specific to Plamodium falciparum...
January 11, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28068960/nutritional-status-among-primary-school-children-in-rural-sri-lanka-a-public-health-challenge-for-a-country-with-high-child-health-standards
#20
N P G C R Naotunna, M Dayarathna, H Maheshi, G S Amarasinghe, V S Kithmini, M Rathnayaka, L Premachandra, N Premarathna, P C Rajasinghe, G Wijewardana, T C Agampodi, S B Agampodi
BACKGROUND: Nutritional status of pre adolescent children is not widely studied in Sri Lanka. The purpose of this study was to determine the nutritional status among pre-adolescent school children in a rural province of Sri Lanka. METHODS: A school based cross sectional study was carried out in North Central Province in 100 rural schools, selected using multi stage cluster sampling with probability proportionate to size. Children in grade one to five were enrolled with a maximum cluster size of fifty...
January 10, 2017: BMC Public Health
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