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Peripheral blood smear

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https://www.readbyqxmd.com/read/28331130/cytopenias-and-clonal-expansion-of-gamma-delta-t-cells-in-a-patient-with-anaplasmosis-a-potential-diagnostic-pitfall
#1
Daniel Marko, Anamarija M Perry, Arjuna Ponnampalam, Michel R Nasr
Human granulocytic anaplasmosis is a rare, tick-borne infectious disease caused by Anaplasma phagocytophilum. Herein, we report a rare case of human granulocytic anaplasmosis associated with cytopenias and clonal expansion of gamma/delta T-cells in the bone marrow. A 77-year old man presented multiple times to the emergency department complaining of muscle weakness. Complete blood count detected cytopenias and peripheral blood smear showed pseudo Pelger-Huet neutrophils. These findings prompted bone marrow evaluation with ancillary studies including flow cytometry, karyotyping and T-cell rearrangement studies...
2017: Journal of Clinical and Experimental Hematopathology: JCEH
https://www.readbyqxmd.com/read/28327821/disseminated-histoplasmosis-diagnosed-in-a-peripheral-blood-smear
#2
André Luiz Maltos, Evaldo Maia, Cristina da Cunha Hueb Barata de Oliveira
No abstract text is available yet for this article.
January 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28321611/iron-deficiency-and-iron-deficiency-anemia-in-3-5-months-old-breastfed-healthy-infants
#3
Sudarsan Krishnaswamy, Dharmagat Bhattarai, Bhavneet Bharti, Prateek Bhatia, Reena Das, Deepak Bansal
OBJECTIVE: To assess the prevalence of iron deficiency (ID) and iron deficiency anemia (IDA) in predominantly breastfed, 3-5-mo-old infants, born at term, with a birth weight ≥ 2.5 kg. METHODS: The cross-sectional study was conducted in the outpatient department of a tertiary care center from January 2013 through December 2014. INCLUSION CRITERIA: Age: 90-180 d, exclusively/predominantly breastfed, birth weight ≥ 2.5 kg and term gestation...
March 21, 2017: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/28318177/can-soluble-transferrin-receptor-be-used-in-diagnosing-iron-deficiency-anemia-and-assessing-iron-response-in-infants-with-moderate-acute-malnutrition
#4
Bahar Bu Yu Kkaragöz, Necat A Akgun, Ayse D Bulus, Sultan Durmus Aydogdu, Cengiz Bal
OBJECTIVE: To evaluate the efficacy of soluble transferrin receptor (sTfR) in diagnosing iron deficiency anemia (IDA) and evaluating iron response in infants with moderate acute malnutrition (MAM). POPULATION AND METHODS: Infants with hemoglobin (Hb) levels lower than threshold values for anemia for their ages and hypochromic/ microcytic anemia on peripheral smear were recruited. MAM was defined as weight/height z score < -2 to -3. Complete blood count (CBC), iron parameters and sTfR were compared among 41 infants with MAM and anemia (MA group), 32 infants with anemia without MAM (group A), and healthy controls (n= 30)...
April 1, 2017: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/28316415/molecular-and-pathological-studies-on-natural-cases-of-bovine-theileriosis
#5
Anamika Gupta, Kuldip Gupta, Geeta Devi Leishangthem, Mandeep Singh Bal, N K Sood, Amarjit Singh
The present communication is a part of study conducted on 32 cases of bovine lymphadenopathies. Out of which, six cases of bovine theileriosis were diagnosed on the basis of peripheral blood smear examination and gross lesions in lymph nodes and visceral organs. Nested PCR using two primer sets N516/N517 and 14136/294 was conducted on samples collected from affected lymph nodes confirms Theileria annulata infection in five out of six cases. Sequencing analysis of amplified product showed 92, 94 and 93 % homology of isolate TH1_Bovine, TH2_Bovine and THEN_Bovine for T...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28304254/clinical-trypanosoma-cruzi-disease-after-cardiac-transplantation-in-a-cynomolgus-macaque-macaca-fascicularis
#6
Elana R Rybak, Steve Shipley, Ivan Tatarov, Tianshu Zhang, Wenji Sun, Gheorghe Braileanu, Lars Burdorf, Evelyn Sievert, Agnes M Azimzadeh, Louis J DeTolla, Richard N Pierson Iii
A cynomolgus macaque received a heterotopic cardiac allograft as part of a transplant study, with monoclonal antibodies targeted to specific immune costimulation molecules (CD154, CD28) but no traditional immunosuppressive therapy after surgery. Clinical anemia was detected on postoperative day (POD) 35 and had worsened (Hgb, 2.3 g/dL; Hct = 7.3%) by POD 47, despite type-matched whole-blood transfusions. After a total of 4 blood transfusions, hematologic parameters were improved (Hgb, 5.9 g/dL; Hct, 18.7%)...
December 1, 2016: Comparative Medicine
https://www.readbyqxmd.com/read/28296756/transient-appearance-of-edta-dependent-pseudothrombocytopenia-in-a-postoperative-patient-with-sepsis-a-case-report
#7
Xiang Shi, Zhongyuan Lin, Liyan He, Wenchao Li, Lijun Mo, Yinyin Li, Zheng Yang, Wu-Ning Mo
RATIONALE: Ethylenediaminetetraacetic acid-dependent pseudothrombocytopenia (EDTA-PTCP) is a rare phenomenon characterized by spuriously low platelet counts when EDTA reacts with harvested blood. However, to the best of our knowledge, only two cases involving EDTA-PTCP in postoperative patients with sepsis have been reported. Here, we describe a case of EDTA-PTCP that appeared transiently in a postoperative patient with sepsis. PATIENT CONCERNS: A 68-year-old female patient underwent laparoscopic tension-free hernioplasty for incisional hernia...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28290970/severe-systemic-lead-toxicity-resulting-from-extra-articular-retained-shrapnel-presenting-as-jaundice-and-hepatitis-a-case-report-and-review-of-the-literature
#8
Ian A Grasso, Matthew R Blattner, Tyler Short, John W Downs
INTRODUCTION: Despite greater than 60,000 nonfatal firearm injuries per year in the United States, retained shrapnel is a relatively rare cause of systemic lead toxicity with less than 100 cases reported in the medical literature since 1867. While intra-articular retained shrapnel as a cause of lead toxicity is well-described, extra-articular fragments are less well known to cause symptomatic disease. CASE REPORT: A 31-year-old man initially presented with abdominal pain, constipation, jaundice, and elevated liver transaminases approximately 3 weeks after suffering a left lower extremity injury during athletic activity...
March 2017: Military Medicine
https://www.readbyqxmd.com/read/28289585/the-mefv-gene-pathogenic-variants-and-phenotype-genotype-correlation-in-children-with-familial-mediterranean-fever-in-the-%C3%A3-anakkale-population
#9
F Battal, F Silan, N Topaloğlu, H Aylanç, Ş Yıldırım, F Köksal Binnetoğlu, M Tekin, N Kaymaz, O Ozdemir
The aim of the current study was to determine the frequency of the Mediterranean fever (MEFV) gene pathogenic variants in 60 children diagnosed with familial Mediterranean fever (FMF) and to compare the phenotype-genotype correlation. Genomic DNA was isolated by the spin-column method from peripheral blood samples (collected in vacutainers containing EDTA) and buccal smears. The MEFV gene profiles for the current FMF cohort were genotyped by pyrosequencing and direct Sanger sequencing techniques for the target pathogenic variants...
December 1, 2016: Balkan Journal of Medical Genetics: BJMG
https://www.readbyqxmd.com/read/28282710/early-onset-friedreich-s-ataxia-with-oculomotor-apraxia
#10
Amene Saghazadeh, Sina Hafizi, Firouzeh Hosseini, Mahmoud Reza Ashrafi, Nima Rezaei
Friedreich's ataxia (FRDA) is a rare autosomal recessive spinocerebellar ataxia which in the majority of cases is associated with a GAA-trinucleotide repeat expansion in the first intron of Frataxin gene located on chromosome 9. The clinical features include progressive gait and limb ataxia, cerebellar dysarthria, neuropathy, optic atrophy, and loss of vibration and proprioception. Ataxia with ocular motor apraxia type 1 (AOA1) is another autosomal recessive cerebellar ataxia which is associated with oculomotor apraxia, hypoalbuminaemia, and hypercholesterolemia...
February 2017: Acta Medica Iranica
https://www.readbyqxmd.com/read/28279619/led-fluorescence-microscopy-novel-method-for-malaria-diagnosis-compared-with-routine-methods
#11
Riddhi Hathiwala, Preeti R Mehta, Gita Nataraj, Siddhi Hathiwala
Rapid and accurate diagnosis of malaria is the need of hour for effective management and controlling drug resistance. The conventional and gold-standard method, Light microscopy (LM), is time-consuming, requires trained staff and well-maintained equipments. The newly developed, rapid diagnostic tests (RDT) are fast and reliable, but give only qualitative results, are expensive and have short shelf life. Light Emission Diode fluorescence microscopy (LED FM) may provide a reliable alternative which can be used for routine diagnosis...
March 6, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28275014/severe-lead-toxicity-attributed-to-bullet-fragments-retained-in-soft-tissue
#12
Debora Weiss, Don Lee, Ryan Feldman, Kate E Smith
A man aged 30 years presented to an emergency department with a 1 month history of severe abdominal pain, jaundice, constipation, lower extremity weakness and weight loss. A peripheral blood smear was performed that showed basophilic stippling of erythrocytes prompting a blood lead level (BLL) evaluation. The patient had a BLL of >200 µg/dL. Retained bullet fragments were identified in the left lower extremity from a previous gunshot wound 10 years prior. Lead from the excised bullet fragment was consistent with the patient's blood lead by isotope ratio analysis...
March 8, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28274003/comparative-study-on-antenatal-and-perinatal-outcome-of-vivax-and-falciparum-malaria-in-a-tertiary-care-hospital-of-kolkata-india
#13
Mousumi Datta, Jhuma Biswas, Shyamal Dasgupta, Kaushik Banerjee, Subhendu Choudhury, Sandip Kumar Sengupta, Prakash Das
INTRODUCTION: Malaria occurring in pregnancy is associated with considerable maternal and perinatal morbidity. In India, the problem is compounded by dual parasitological aetiology of Plasmodiumvivax (P.vivax) and Plasmodium falciparum (P.falciparum). AIM: To compare the outcome of infections by P. vivax and P.falciparum species among pregnant women in a hospital setting. MATERIALS AND METHODS: Pregnant women who tested positive for malaria either by microscopy of peripheral blood smear or ELISA test for double antigen were enrolled in the study...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28271682/fungal-spores-affect-peripheral-blood-counting-in-sysmex-5000i-automated-hematology-counter-and-relative-case-report
#14
Zhang Xiao-Lei, Deng Fang, Shan Wu-Lin, Ge Xin, Li Ming
BACKGROUND: Fungal spores can be observed on peripheral blood smears and may affect cell counting in an automated hematology counter. The purpose of this study was to evaluate the effects of fungal spores on cell counting in Sysmex-5000i automated hematology counter. METHODS: The common clinical fungal spores we selected were C. albicans, C. krusei, C. tropicalis, and C. glabrata, using 0.9% normal saline to formulate a final concentration of 4 - 6 x 109/L fungal liquid, mixed with peripheral blood (containing high, medium and low concentrations of white blood cells and platelets), and formulated to different concentrations (4 - 6 x 108/L, 4 - 6 x 107/L, 4 - 6 x 106/L, 4 - 6 x 105/L)...
March 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28264705/improved-molecular-detection-of-babesia-infections-in-animals-using-a-novel-quantitative-real-time-pcr-diagnostic-assay-targeting-mitochondrial-dna
#15
Barbara A Qurollo, Nikole R Archer, Megan E Schreeg, Henry S Marr, Adam J Birkenheuer, Kaitlin N Haney, Brittany S Thomas, Edward B Breitschwerdt
BACKGROUND: Babesiosis is a protozoal, tick transmitted disease found worldwide in humans, wildlife and domesticated animals. Commonly used approaches to diagnose babesiosis include microscopic examination of peripheral blood smears, detection of circulating antibodies and PCR. To screen and differentiate canine Babesia infections many PCR assays amplify the 18S rRNA gene. These sequences contain hypervariable regions flanked by highly conserved regions allowing for amplification of a broad-range of Babesia spp...
March 7, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28255016/prognostic-impact-of-circulating-plasma-cells-in-patients-with-multiple-myeloma-implications-for-plasma-cell-leukaemia-definition
#16
Miquel Granell, Xavier Calvo, Antoni Garcia-Guiñón, Lourdes Escoda, Eugènia Abella, Clara M Martínez, Montserrat Teixidó, Maria Teresa Gimenez, Alicia Senín, Patricia Sanz, Desirée Campoy, Ana Vicent, Leonor Arenillas, Laura Rosiñol, Jorge Sierra, Joan Blade, Carlos Fernández de Larrea
The presence of circulating plasma cells in patients with multiple myeloma is considered a marker for highly proliferative disease. In the present study, the impact of circulating plasma cells assessed by cytology on survival of patients with multiple myeloma was analysed. Wright-Giemsa stained peripheral blood smears of 482 patients with newly diagnosed myeloma or plasma cell leukaemia were reviewed and patients were classified in four categories according to the percentage of circulating plasma cells: 0%, 1-4%, 5-20% and plasma cell leukemia with the following frequencies: 382 (79...
March 2, 2017: Haematologica
https://www.readbyqxmd.com/read/28250930/disseminated-histoplasmosis-a-rare-clinical-phenotype-with-difficult-diagnosis
#17
Xiao-Feng Xiong, Li-Li Fan, Mei Kang, Jia Wei, De-Yun Cheng
We describe a rare and interesting case of a 37-year-old man who presented with an intermittent fever, progressive cytopenia, and hepatosplenomegaly. Histopathological examination of a bone marrow smear revealed haemophagocytes and intracellular yeast-like Histoplasma capsulatum (H. capsulatum); thus, we prolonged the blood culture duration to detect fungi, and H. capsulatum was detected in the peripheral blood. After the diagnosis of disseminated histoplasmosis, the patient was successfully treated with amphotericin B and symptomatic therapy...
May 2017: Respirology Case Reports
https://www.readbyqxmd.com/read/28245368/-clinical-and-immunophenotypic-properties-of-small-cell-variant-of-t-cell-prolymphocytic-leukemia
#18
Ya-Ping Yu, Li-Ping Wang, Ping Song, Jian-Gang Mei, Zhi-Ming An, Xiao-Gang Zhou, Feng Li, Yu-Mei Tang, Yong-Ping Zhai
OBJECTIVE: To investigate the clinical, morphologic and immunophenotypic properties of the patients with small cell variant of T-cell prolymphocytic leukaemia(T-PLL). METHODS: Peripheral blood and bone marrow cytomorphologic and immunophenotypic examination, and T-cell receptor(TCR) gene rearrangement detection were used to verify the diagnosis for 2 patients with lymphocytosis. Two patients were treated with combined chemotherapeutic protocol based on fludarabine...
February 2017: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/28194178/role-of-helicobacter-pylori-eradication-therapy-on-platelet-recovery-in-chronic-immune-thrombocytopenic-purpura
#19
Khan Sheema, Ujjan Ikramdin, Naz Arshi, Naz Farah, Sheikh Imran
Background. Idiopathic thrombocytopenic purpura (ITP) is a bleeding disorder in which the immune system destroys native platelets. In this condition an autoantibody is generated against a platelet antigen. ITP affects women more often than men and is more common in children than adults. Objective. To assess the effect of Helicobacter pylori eradication therapy (HPET) on platelet count in Helicobacter pylori associated chronic immune thrombocytopenic purpura (chronic ITP) in adult. Materials and Methods. It is an interventional prospective study conducted at Liaquat University of Medical and Health Sciences, Jamshoro, from 2014 to 2015...
2017: Gastroenterology Research and Practice
https://www.readbyqxmd.com/read/28193504/strong-anti-epstein-barr-virus-ebv-or-cytomegalovirus-cmv-cellular-immune-responses-predict-survival-and-a-favourable-response-to-anti-tuberculosis-therapy
#20
REVIEW
Tumaini Nagu, Said Aboud, Martin Rao, Mecky Matee, Rebecca Axelsson, Davide Valentini, Ferdinand Mugusi, Alimuddin Zumla, Markus Maeurer
BACKGROUND: Intact immune responses to cytomegalovirus (CMV) and Epstein-Barr virus (EBV) represent a biologically and clinically relevant correlate of 'immunological fitness' in humans. However, there is a lack of knowledge concerning anti-EBV or anti-CMV responses in patients with pulmonary tuberculosis (TB), in whom aberrant immune responses may promote progression of clinical disease. METHODS: Venous blood samples were obtained at the time of (sputum smear positive) pulmonary TB diagnosis...
February 11, 2017: International Journal of Infectious Diseases: IJID
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