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https://www.readbyqxmd.com/read/29327727/generation-of-a-precise-oct4-hrgfp-knockin-cynomolgus-monkey-model-via-crispr-cas9-assisted-homologous-recombination
#1
Yiqiang Cui, Yuyu Niu, Jiankui Zhou, Yongchang Chen, Yiwei Cheng, Shangang Li, Zongyong Ai, Chu Chu, Hong Wang, Bo Zheng, Xuejin Chen, Jiahao Sha, Xuejiang Guo, Xingxu Huang, Weizhi Ji
No abstract text is available yet for this article.
January 12, 2018: Cell Research
https://www.readbyqxmd.com/read/29322514/the-yin-and-yang-of-yy1-in-tumor-growth-and-suppression
#2
REVIEW
Levon M Khachigian
Yin Yang-1 (YY1) is a zinc finger protein and member of the GLI-Kruppel family that can activate or inactivate gene expression depending on interacting partners, promoter context and chromatin structure, and may be involved in the transcriptional control of ∼10% of the total mammalian gene set. A growing body of literature indicates that YY1 is overexpressed in multiple cancer types and that increased YY1 levels correlate with poor clinical outcomes in many cancers. However the role of YY1 in the promotion or suppression of tumor growth remains controversial and its regulatory effects may be tumor cell type dependent at least in experimental systems...
January 11, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29321622/evaluation-of-a-developmental-hierarchy-for-breast-cancer-cells-to-assess-risk-based-patient-selection-for-targeted-treatment
#3
Sarah A Bliss, Sunirmal Paul, Piotr W Pobiarzyn, Seda Ayer, Garima Sinha, Saumya Pant, Holly Hilton, Neha Sharma, Maria F Cunha, Daniel J Engelberth, Steven J Greco, Margarette Bryan, Magdalena J Kucia, Sham S Kakar, Mariusz Z Ratajczak, Pranela Rameshwar
This study proposes that a novel developmental hierarchy of breast cancer (BC) cells (BCCs) could predict treatment response and outcome. The continued challenge to treat BC requires stratification of BCCs into distinct subsets. This would provide insights on how BCCs evade treatment and adapt dormancy for decades. We selected three subsets, based on the relative expression of octamer-binding transcription factor 4 A (Oct4A) and then analysed each with Affymetrix gene chip. Oct4A is a stem cell gene and would separate subsets based on maturation...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321513/tousled-like-kinase-1-is-a-negative-regulator-of-core-transcription-factors-in-murine-embryonic-stem-cells
#4
Jina Lee, Min Seong Kim, Su Hyung Park, Yeun Kyu Jang
Although the differentiation of pluripotent cells in embryonic stem cells (ESCs) is often associated with protein kinase-mediated signaling pathways and Tousled-like kinase 1 (Tlk1) is required for development in several species, the role of Tlk1 in ESC function remains unclear. Here, we used mouse ESCs to study the function of Tlk1 in pluripotent cells. The knockdown (KD)-based Tlk1-deficient cells showed that Tlk1 is not essential for ESC self-renewal in an undifferentiated state. However, Tlk1-KD cells formed irregularly shaped embryoid bodies and induced resistance to differentiation cues, indicating their failure to differentiate into an embryoid body...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29320691/network-features-and-dynamical-landscape-of-naive-and-primed-pluripotency
#5
Benjamin Pfeuty, Clémence Kress, Bertrand Pain
Although the broad and unique differentiation potential of pluripotent stem cells relies on a complex transcriptional network centered around Oct4, Sox2, and Nanog, two well-distinct pluripotent states, called "naive" and "primed", have been described in vitro and markedly differ in their developmental potential, their expression profiles, their signaling requirements, and their reciprocal conversion. Aiming to determine the key features that segregate and coordinate these two states, data-driven optimization of network models is performed to identify relevant parameter regimes and reduce network complexity to its core structure...
January 9, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29319535/scalable-and-physiologically-relevant-microenvironments-for-human-pluripotent-stem-cell-expansion-and-differentiation
#6
Qiang Li, Haishuang Lin, Qian Du, Kan Liu, Ou Wang, Catelyn Antionette Evans, Hannah Marie Christian, Chi Zhang, Yuguo Lei
Human pluripotent stem cells (hPSCs) are required in large numbers for various biomedical applications. However, the scalable and cost-effective culturing of high quality hPSCs and their derivatives remains very challenging. Here, we report a novel and physiologically relevant 3D culture system (called the AlgTube cell culture system) for hPSC expansion and differentiation. With this system, cells are processed into and cultured in microscale alginate hydrogel tubes that are suspended in the cell culture medium in a culture vessel...
January 10, 2018: Biofabrication
https://www.readbyqxmd.com/read/29317833/clinicopathological-and-prognostic-significance-of-oct4-in-patients-with-hepatocellular-carcinoma-a-meta-analysis
#7
REVIEW
Chaojie Liang, Yingchen Xu, Hua Ge, Guangming Li, Jixiang Wu
Background and aims: Octamer-binding transcription factor 4 (OCT4) has been implicated in the development of hepatocellular carcinoma (HCC), although the findings are controversial. We conducted a meta-analysis to assess the correlation between OCT4 and the clinicopathological characteristics and the prognostic value in HCC. Methods: An electronic search for relevant articles was conducted in PubMed, Cochrane Library, Web of Science, EMBASE database, Chinese CNKI, and Chinese WanFang database...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29317250/arctigenin-protects-against-ultraviolet-a-induced-damage-to-stemness-through-inhibition-of-the-nf-%C3%AE%C2%BAb-mapk-pathway
#8
See-Hyoung Park, Jae Youl Cho, Sae Woong Oh, Mingyeong Kang, Seung Eun Lee, Ju Ah Yoo, Kwangseon Jung, Jienny Lee, Sang Yeol Lee, Jongsung Lee
The stemness of stem cells is negatively affected by ultraviolet A (UVA) irradiation. This study was performed to examine the effects of arctigenin on UVA-irradiation-induced damage to the stemness of human mesenchymal stem cells (hMSCs) derived from adipose tissue. The mechanisms of action of arctigenin were also investigated. A BrdU-incorporation assay demonstrated that arctigenin attenuated the UVA-induced reduction of the cellular proliferative potential. Arctigenin also increased the UVA-induced reduction in stemness of hMSCs by upregulating stemness-related genes such as SOX2, OCT4, and NANOG...
January 6, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29312817/murine-pluripotent-stem-cells-that-escape-differentiation-inside-teratomas-maintain-pluripotency
#9
Yangli Pei, Liang Yue, Wei Zhang, Jinzhu Xiang, Zhu Ma, Jianyong Han
Background: Pluripotent stem cells (PSCs) offer immense potential as a source for regenerative therapies. The teratoma assay is widely used in the field of stem cells and regenerative medicine, but the cell composition of teratoma is still elusive. Methods: We utilized PSCs expressing enhanced green fluorescent protein (EGFP) under the control of the Pou5f1 promoter to study the persistence of potential pluripotent cells during teratoma formation in vivo. OCT4-MES (mouse embryonic stem cells) were isolated from the blastocysts of 3...
2018: PeerJ
https://www.readbyqxmd.com/read/29307582/igf-1r-promotes-symmetric-self-renewal-and-migration-of-alkaline-phosphatase-germ-stem-cells-through-hif-2%C3%AE-oct4-cxcr4-loop-under%C3%A2-hypoxia
#10
Yung-Che Kuo, Heng-Kien Au, Jue-Liang Hsu, Hsiao-Feng Wang, Chiung-Ju Lee, Syue-Wei Peng, Ssu-Chuan Lai, Yu-Chih Wu, Hong-Nerng Ho, Yen-Hua Huang
Hypoxia cooperates with endocrine signaling to maintain the symmetric self-renewal proliferation and migration of embryonic germline stem cells (GSCs). However, the lack of an appropriate in vitro cell model has dramatically hindered the understanding of the mechanism underlying this cooperation. Here, using a serum-free system, we demonstrated that hypoxia significantly induced the GSC mesenchymal transition, increased the expression levels of the pluripotent transcription factor OCT4 and migration-associated proteins (SDF-1, CXCR4, IGF-1, and IGF-1R), and activated the cellular expression and translocalization of the CXCR4-downstream proteins ARP3/pFAK...
January 3, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29305964/in-vitro-and-in-vivo-characterization-of-stem-like-cells-from-canine-osteosarcoma-and-assessment-of-drug-sensitivity
#11
Monica Gatti, Agnese Solari, Alessandra Pattarozzi, Chiara Campanella, Stefano Thellung, Lorella Maniscalco, Raffaella De Maria, Roberto Würth, Alessandro Corsaro, Adriana Bajetto, Alessandra Ratto, Angelo Ferrari, Antonio Daga, Federica Barbieri, Tullio Florio
CSC self-renewing and drug resistance cause treatment failure and tumor recurrence. Osteosarcoma is an aggressive bone tumor characterized by biological and molecular heterogeneity, possibly dependent on cancer stem cells (CSCs). CSC identification in osteosarcoma and their efficient targeting are still open questions. Spontaneous canine osteosarcoma shares clinical and biological features with the human tumors, representing a model for translational studies. We characterized three CSC-enriched canine osteosarcoma cultures...
January 3, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29304842/erk-inhibition-promotes-neuroectodermal-precursor-commitment-by-blocking-self-renewal-and-primitive-streak-formation-of-the-epiblast
#12
Yang Yu, Xiaoxiao Wang, Xiaoxin Zhang, Yanhua Zhai, Xukun Lu, Haixia Ma, Kai Zhu, Tongbiao Zhao, Jianwei Jiao, Zhen-Ao Zhao, Lei Li
BACKGROUND: Pluripotent stem cells hold great promise for regenerative medicine. However, before clinical application, reproducible protocols for pluripotent stem cell differentiation should be established. Extracellular signal-regulated protein kinase (ERK) signaling plays a central role for the self-renewal of epiblast stem cells (EpiSCs), but its role for subsequent germ layer differentiation is still ambiguous. We proposed that ERK could modulate differentiation of the epiblast. METHODS: PD0325901 was used to inhibit ERK activation during the differentiation of embryonic stem cells and EpiSCs...
January 5, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29299196/extract-of-mouse-embryonic-stem-cells-induces-the-expression-of-pluripotency-genes-in-human-adipose-tissue-derived-stem-cells
#13
Paria Motamen Salehi, Tahereh Foroutan, Arash Javeri, Masoumeh Fakhr Taha
Objectives: In some previous studies, the extract of embryonic carcinoma cells (ECCs) and embryonic stem cells (ESCs) have been used to reprogram somatic cells to more dedifferentiated state. The aim of this study was to investigate the effect of mouse ESCs extract on the expression of some pluripotency markers in human adipose tissue-derived stem cells (ADSCs). Materials and Methods: Human ADSCs were isolated from subcutaneous abdominal adipose tissue and characterized by flow cytometric analysis for the expression of some mesenchymal stem cell markers and adipogenic and osteogenic differentiation...
November 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29299179/lncrnas-and-their-role-in-cancer-stem-cells
#14
REVIEW
Shusen Chen, Jiamin Zhu, Feng Wang, Zhifeng Guan, Yangyang Ge, Xi Yang, Jing Cai
Cancer stem cells (CSCs) play a vital role in the formation of tumors and have been studied as a target of anticancer therapy. Long non-coding RNAs (lncRNAs) are important in the genesis and progression of cancer. Various lncRNAs, such as ROR, HOTAIR, H19, UCA1, and ARSR, are involved in cancer stemness. These lncRNAs could regulate the expression of CSC-related transcriptional factors, such as SOX2, OCT4, and NANOG, in colorectal, prostate, bladder, breast, liver, and other cancer types. In this work, we review the progress of lncRNAs and cancer stem cells and discuss the potential signal pathways of lncRNAs in cancer stemness...
December 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/29297135/stem-cell-factor-supports-migration-in-canine-mesenchymal-stem-cells
#15
Nathaly Enciso, Luciana L K Ostronoff, Guillermo Mejías, Leticia G León, María Luisa Fermín, Elena Merino, Cristina Fragio, Luis Avedillo, Concepción Tejero
Adult Mesenchymal Stem Cells (MSC) are cells that can be defined as multipotent cells able to differentiate into diverse lineages, under appropriate conditions. These cells have been widely used in regenerative medicine, both in preclinical and clinical settings. Initially discovered in bone marrow, MSC can now be isolated from a wide spectrum of adult and foetal tissues. Studies to evaluate the therapeutic potential of these cells are based on their ability to arrive to damaged tissues. In this paper we have done a comparative study analyzing proliferation, surface markers and OCT4, SOX9, RUNX2, PPARG genes expression in MSC cells from Bone marrow (BMMSC) and Adipose tissue (ASC)...
January 3, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29286621/quasi-stem-cells-derived-from-human-somatic-cells-by-chemically-modified-carbon-nanotubes
#16
Jae-Hyeok Lee, Hyuk-Kwon Kwon, Hyeon-Jun Shin, Gwang-Hyeon Nam, Jae-Ho Kim, Sangdun Choi
Surface modification of micro- and nanotopography has been employed to alter the surface properties of scaffolds for controlling cell attachment, proliferation, and differentiation. This study reports a method for generating multinucleated colonies as evidenced by spherical colony formation through nanotopography-induced expression of reprogramming factors in human dermal fibroblasts. Colony formation is achieved by subjecting the cells to specific environments, such as culturing with single-walled carbon nanotubes and poly-L-lysine (PLL-SWCNTs)...
December 29, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29281720/reversible-dual-inhibitor-against-g9a-and-dnmt1-improves-human-ipsc-derivation-enhancing-met-and-facilitating-transcription-factor-engagement-to-the-genome
#17
Juan Roberto Rodriguez-Madoz, Edurne San Jose-Eneriz, Obdulia Rabal, Natalia Zapata-Linares, Estibaliz Miranda, Saray Rodriguez, Angelo Porciuncula, Amaia Vilas-Zornoza, Leire Garate, Victor Segura, Elizabeth Guruceaga, Xabier Agirre, Julen Oyarzabal, Felipe Prosper
The combination of defined factors with small molecules targeting epigenetic factors is a strategy that has been shown to enhance optimal derivation of iPSCs and could be used for disease modelling, high throughput screenings and/or regenerative medicine applications. In this study, we showed that a new first-in-class reversible dual G9a/DNMT1 inhibitor compound (CM272) improves the efficiency of human cell reprogramming and iPSC generation from primary cells of healthy donors and patient samples, using both integrative and non-integrative methods...
2017: PloS One
https://www.readbyqxmd.com/read/29280266/senescence-promotes-in%C3%A2-vivo-reprogramming-through-p16ink4a-and-il-6
#18
Lluc Mosteiro, Cristina Pantoja, Alba de Martino, Manuel Serrano
Cellular senescence is a damage response aimed to orchestrate tissue repair. We have recently reported that cellular senescence, through the paracrine release of interleukin-6 (IL6) and other soluble factors, strongly favors cellular reprogramming by Oct4, Sox2, Klf4, and c-Myc (OSKM) in nonsenescent cells. Indeed, activation of OSKM in mouse tissues triggers senescence in some cells and reprogramming in other cells, both processes occurring concomitantly and in close proximity. In this system, Ink4a/Arf-null tissues cannot undergo senescence, fail to produce IL6, and cannot reprogram efficiently; whereas p53-null tissues undergo extensive damage and senescence, produce high levels of IL6, and reprogram efficiently...
December 27, 2017: Aging Cell
https://www.readbyqxmd.com/read/29277003/eugenol-enhances-proliferation-and-migration-of-mouse-bone-marrow-derived-mesenchymal-stem-cells-in-vitro
#19
Sajjad Sisakhtnezhad, Mojdeh Heidari, Ali Bidmeshkipour
Mesenchymal stem cells (MSCs) have received considerable attention in regenerative medicine during the past decade. Eugenol is a natural and versatile vegetable molecule, which has a wide variety of therapeutic effects. Although different biological and pharmaceutical functions of Eugenol are well known, its effect on MSCs has not been studied yet. Therefore, this study was focused on investigating the effect of Eugenol on the proliferation and migration of bone marrow (BM)-derived MSCs in vitro. To do so, BM-MSCs were isolated from 4 to 8 weeks old NMRI mice...
December 17, 2017: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29275121/ve-cadherin-regulates-the-self-renewal-of-mouse-embryonic-stem-cells-via-lif-stat3-signaling-pathway
#20
Ningning He, Xiaoniao Chen, Dan Wang, Ke Xu, Lingling Wu, Yuanyuan Liu, Hongyan Tao, Qinjun Zhao, Xiaocang Cao, Yuhao Li, Na Liu, Xin Qi, Zhongchao Han, Deling Kong, Jun Yang, Zongjin Li
With the abilities of self-renewal and differentiation, embryonic stem (ES) cells provide an unlimited source for stem cell-based therapeutics. However, the maintenance of ES cells with mouse embryonic fibroblasts (MEFs) can limit the clinical translation of ES cells. In the present study, we synthesized a fusion protein of the immunoglobulin G (IgG) fragment crystallizable region and vascular endothelial cadherin (VE-cadherin) extracellular domain (VE-cad-Fc) as a substrate for mouse ES cell culture, and we hypothesized that VE-cadherin could enhance the pluripotency and self-renewal of ES cells...
December 16, 2017: Biomaterials
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