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https://www.readbyqxmd.com/read/28895868/identification-of-novel-biomarkers-for-treatment-monitoring-in-canine-leishmaniosis-by-high-resolution-quantitative-proteomic-analysis
#1
Silvia Martinez-Subiela, Anita Horvatic, Damian Escribano, Luis Pardo-Marin, Meric Kocaturk, Vladimir Mrljak, Richard Burchmore, Jose J Ceron, Zeki Yilmaz
The objective of this study was to use the Tandem Mass Tag (TMT) isobaric label-based proteomic approach, in order to identify new potential biomarkers for the treatment monitoring of canine leishmaniosis that could not be identified by the use of gel-based techniques. For this purpose serum samples were obtained from 5 clinically diseased dogs before and one month after the treatment of canine leishmaniosis. The non-depleted serum samples were subjected to reduction, alkylation and trypsin digestion, and the resulting peptides were labeled using 6-plex TMT reagents...
September 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28865205/investigation-of-the-bone-metabolism-in-dogs-with-leishmaniasis
#2
M Ari, A Bildik, S Ertug
The present study was aimed at determining bone metabolism related changes in dogs with visseral leishmaniasis such as vitamin D, calcium, phosphate and ALP levels as compared to healthy ones. A total of 40 serums of male dogs aged between 4 and 6 years that were sent to Adnan Menderes University, Faculty of Medicine, Parasitology Laboratory from Kuşadasy and Bodrum districts between the years 2012 and 2013, suspected with leishmaniasis, by the veterinary physicians in accordance with the cold chain rules and 20 of which were evaluated as leishmania seropositive and 20 as leishmania seronegative by the IFA test, were included in this study...
March 1, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28863873/evaluation-of-the-associations-between-immunoglobulin-e-levels-and-the-number-of-natural-teeth
#3
Kyungdo Han, Eunjoo Lee, Jun-Beom Park
BACKGROUND/PURPOSE: The purpose of the study was to evaluate the association between the number of natural teeth and the total serum immunoglobulin E (IgE), allergen-specific IgE against Dermatophagoides farina, allergen-specific IgE against cockroaches, or allergen-specific IgE against dogs with nationally representative data using the Korean National Health and Nutrition Examination Survey (KNHANES). METHODS: This study involved a cross-sectional analysis using KNHANES data from 2010...
August 29, 2017: Journal of the Formosan Medical Association, Taiwan Yi Zhi
https://www.readbyqxmd.com/read/28804921/serum-thymidine-kinase-1-canine-c-reactive-protein-haptoglobin-and-vitamin-d-concentrations-in-dogs-with-immune-mediated-hemolytic-anemia-thrombocytopenia-and-polyarthropathy
#4
M Grobman, H Outi, H Rindt, C Reinero
BACKGROUND: Relapses of immune-mediated hemolytic anemia (IMHA), thrombocytopenia (ITP), or polyarthropathy (IMPA) occur despite normal hematologic and cytologic parameters. Thymidine kinase 1 (TK1), canine C-reactive protein (c-CRP), haptoglobin (HPT), and 25-Hydroxyvitamin-D (25(OH)D) might be adjunct to current monitoring strategies. HYPOTHESIS/OBJECTIVES: Compare serum concentrations of TK1, c-CRP, HPT, and 25(OH)D in dogs with well- and poorly controlled primary IMHA, ITP, or IMPA...
September 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28634899/primary-prevention-of-food-allergy
#5
REVIEW
Rachel L Peters, Melanie R Neeland, Katrina J Allen
PURPOSE OF REVIEW: This article summarises recent developments on the prevention of food allergy in terms of the 5 D's of the development of food allergy: dry skin, diet, dogs, dribble, and vitamin D. RECENT FINDINGS: While several advances have improved our understanding of the development of food allergy, few preventive strategies have been implemented beyond changes in infant feeding guidelines. These now state that the introduction of allergenic solids such as peanuts should occur in the first year of life...
August 2017: Current Allergy and Asthma Reports
https://www.readbyqxmd.com/read/28611427/canine-leishmaniasis-progression-is-associated-with-vitamin-d-deficiency
#6
A Rodriguez-Cortes, C Martori, A Martinez-Florez, A Clop, M Amills, J Kubejko, J Llull, J M Nadal, J Alberola
The relationship between vitamin D deficiency and the risk of suffering from a plethora of health disorders, ranging from autoimmune processes to infectious diseases has been widely described. Nonetheless, the potential role of vitamin D in visceral leishmaniasis remains uncharacterized. In the Mediterranean basin, where the dog is leishmania's main peri-domestic reservoir, control measures against the canine disease have shown beneficial effects on the incidence of human leishmaniasis. In this study, we measured the vitamin D levels in serum samples from a cohort of 68 healthy and disease dogs from a highly endemic area and we have also studied the relationship of these levels with parasitological and immunological parameters...
June 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28576851/urinary-tamm-horsfall-protein-albumin-vitamin-d-binding-protein-and-retinol-binding-protein-as-early-biomarkers-of-chronic-kidney-disease-in-dogs
#7
Fernanda Chacar, Márcia Kogika, Talita R Sanches, Douglas Caragelasco, Cínthia Martorelli, Camila Rodrigues, Jose Manuel C Capcha, Dennis Chew, Lúcia Andrade
Proteinuria is a marker and mediator of chronic kidney disease (CKD). In clinical practice, the urinary protein-to-creatinine ratio (UP/C) is of limited usefulness, because it indicates only the magnitude of proteinuria and not the origin of the loss (glomerular or tubular). The complete assessment of proteinuria includes quantitative and qualitative evaluations, both of which are required in order to optimize the therapy. In addition to measuring the UP/C, we performed SDS-PAGE and western blotting to determine the expression of albumin, vitamin D-binding protein (VDBP), retinol-binding protein (RBP), and Tamm-Horsfall protein (THP) in urine samples of 49 dogs: healthy (control) dogs (n = 9); and dogs with CKD (n = 40), stratified by stage...
June 2017: Physiological Reports
https://www.readbyqxmd.com/read/28419560/fibroblast-growth-factor-23-concentration-in-dogs-with-chronic-kidney-disease
#8
L M Harjes, V J Parker, K Dembek, G S Young, L H Giovaninni, M M Kogika, D J Chew, R E Toribio
BACKGROUND: Chronic kidney disease (CKD) is associated with hyperphosphatemia, decreased vitamin D metabolite concentrations, and hyperparathyroidism. This syndrome is known as CKD-mineral bone disorder (CKD-MBD). Recently, it has been shown that an increase in fibroblast growth factor-23 (FGF-23) concentration is an early biomarker of CKD in people. It is an independent risk factor for both progression of renal disease and survival time in humans and cats with CKD. Information about FGF-23 in healthy dogs and those with CKD is lacking...
May 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28403556/assessment-of-endogenous-25-hydroxyvitamin-d-serum-concentrations-by-liquid-chromatography-tandem-mass-spectrometry-in-various-animal-species
#9
Carol A Fritz, Kimberly A Navetta, David P Wolford, Jennifer L Colangelo
BACKGROUND: Mass spectrometry (MS) has become the preferential method for the analysis of vitamin D in the clinic, yet no single platform is utilized for preclinical species in drug development studies. For vitamin D, the MS platform can provide certain benefits such as applicability of a single assay for multiple species, low cost, and high specificity. OBJECTIVES: A quantitative liquid chromatography-tandem MS (LC-MS/MS) assay for 25-hydroxyvitamin D3 (25OHD3 ) and D2 (25OHD2 ) was validated for rat, dog, mouse, and monkey, and suitability for drug development studies was assessed...
June 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28390394/hypovitaminosis-d-is-associated-with-negative-outcome-in-dogs-with-protein-losing-enteropathy-a-retrospective-study-of-43-cases
#10
K Allenspach, J Rizzo, A E Jergens, Y M Chang
BACKGROUND: Hypovitaminosis D has previously been shown to be prevalent amongst dogs with protein losing enteropathy (PLE). The hypothesis of this study was that Low 25-hydroxyvitamin D (25(OH) D) serum concentrations could be a risk factor for negative outcome in dogs with PLE. Forty-three dogs diagnosed with PLE (2005-2014) and which serum Vitamin D serum concentrations were collected and archived at -80 Degrees C were analyzed. Post-diagnostic communication with referring veterinarians was made to determine outcome of PLE dogss: Dogs which died due to PLE within 4 months after diagnosis (negative outcome group, n = 22) and dogs alive or which died due to another disease at the end point of the study (1 year after diagnosis, good outcome group, n = 21)...
April 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28294587/vitamin-d-levels-and-allergic-diseases-an-italian-cross-sectional-multicenter-survey
#11
MULTICENTER STUDY
C Lombardi, G Passalacqua
Background. During the two last decades, the interest in the role of Vitamin D (VD) in allergic disease has increased. Apart from the well-known actions of VD in bone metabolism, recent studies suggested its possible role as an immune-modulator in allergy. Objective. This study, conducted over the Italian territory, evaluated the possible correlations between VD serum level and diagnosed allergic diseases (rhinitis / asthma, food allergy, atopic dermatitis). Thus, VD was assessed in patients with physician-diagnosed allergic diseases...
March 2017: European Annals of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28186657/association-of-vitamin-d-metabolites-with-parathyroid-hormone-fibroblast-growth-factor-23-calcium-and-phosphorus-in-dogs-with-various-stages-of-chronic-kidney-disease
#12
V J Parker, L M Harjes, K Dembek, G S Young, D J Chew, R E Toribio
BACKGROUND: Hypovitaminosis D is associated with progression of renal disease, development of renal secondary hyperparathyroidism (RHPT), chronic kidney disease-mineral bone disorder (CKD-MBD), and increased mortality in people with CKD. Despite what is known regarding vitamin D dysregulation in humans with CKD, little is known about vitamin D metabolism in dogs with CKD. OBJECTIVES: The purpose of our study was to further elucidate vitamin D status in dogs with different stages of CKD and to relate it to factors that affect the development of CKD-MBD, including parathyroid hormone (PTH), fibroblast growth factor-23 (FGF-23), calcium, and phosphorus concentrations...
May 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/27928177/reduction-of-serum-25-hydroxyvitamin-d-concentrations-with-intravenous-lipid-emulsion-in-a-dog
#13
Brittany Heggem Perry, Maureen McMichael, Markus Rick, Emily Jewell
The recommended daily allowance of vitamin D has been increased. Toxicosis in pets may increase as a result. A dog ingested ~ 200 000 IU of vitamin D, serum concentrations were above the reference range (RR) and decreased to the RR after lipid treatment. This is the first known report of lipid treatment for hypervitaminosis D.
December 2016: Canadian Veterinary Journal. la Revue Vétérinaire Canadienne
https://www.readbyqxmd.com/read/27810204/serum-25-hydroxyvitamin-d-concentrations-in-dogs-with-osteosarcoma-do-not-differ-from-those-of-age-and-weight-matched-control-dogs
#14
Jennifer L Willcox, Catherine Hammett-Stabler, Marlene L Hauck
Vitamin D concentrations show an inverse correlation with incidence of certain tumors in people and dogs. Additionally, human osteosarcoma has been associated with dysregulation of vitamin D-dependent pathways. The study objective was to compare serum 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in 20 dogs with osteosarcoma to age- and weight-matched control dogs. We hypothesized that dogs with osteosarcoma would have lower serum 25-hydroxyvitamin D than control dogs. The mean 25-hydroxyvitamin D3 concentrations for dogs with osteosarcoma and matched-controls were 34...
November 2016: Veterinary Journal
https://www.readbyqxmd.com/read/27632366/evaluation-and-comparison-of-vitamin-d-responsive-gene-expression-in-ovine-canine-and-equine-kidney
#15
Sara Azarpeykan, Keren E Dittmer, Jonathan C Marshall, Kalyani C Perera, Erica K Gee, Els Acke, Keith G Thompson
The aim of this study was to determine the relative abundance and relationship of vitamin D responsive and calcium transporting transcripts (TRPV5, TRPV6, calD9k, calD28k, PMCA, NCX1, CYP27B1, CYP24A1, and VDR) in ovine, canine and, equine kidney using quantitative real-time PCR (RT-qPCR), and then perform a comparison between the three species. Renal tissue samples were harvested post-mortem from 10 horses, 10 sheep, and five dogs. Primers were designed for each gene. For each sample total RNA was extracted, cDNA synthesised, and RT-qPCR was performed...
2016: PloS One
https://www.readbyqxmd.com/read/27547394/oral-vitamin-d-supplementation-at-five-times-the-recommended-allowance-marginally-affects-serum-25-hydroxyvitamin-d-concentrations-in-dogs
#16
Lauren R Young, Robert C Backus
Little is known regarding optimal vitamin D status in adult dogs. To date no studies on vitamin D supplementation for improving vitamin D status have been reported for adult dogs. The aims of this study were to identify dogs with low vitamin D status and evaluate an oral dosage of cholecalciferol (D3) for effectiveness in increasing vitamin D status. For this, forty-six privately owned dogs were evaluated. Of the dogs, thirty-three (or 71·7 %) had serum 25-hydroxyvitamin D (25(OH)D) concentrations less than 100 ng/ml, a minimum previously suggested for vitamin D sufficiency in dogs...
2016: Journal of Nutritional Science
https://www.readbyqxmd.com/read/27470342/cooperative-antiproliferative-and-differentiation-enhancing-activity-of-medicinal-plant-extracts-in-acute-myeloid-leukemia-cells
#17
Gulzhan T Zhamanbayeva, Araylim N Aralbayeva, Maira K Murzakhmetova, Sultan T Tuleukhanov, Michael Danilenko
Acute myeloid leukemia (AML) is an aggressive hematopoietic malignancy with poor prognosis and limited treatment options. Sea buckthorn (Hippophae rhamnoides) berries, dog rose (Rosa canina) rosehips, and garden sage (Salvia officinalis) and oregano (Origanum vulgare) aerial parts are widely used in traditional medicine and exhibit antitumor effects in preclinical models. However, these plants remain scarcely tested for antileukemic activity. Here, we show that their water-ethanol leaf extracts reduced the growth and viability of AML cells and, at non-cytotoxic doses, potentiated cell differentiation induced by a low concentration of 1α,25-dihydroxyvitamin D3, the hormonal form of vitamin D, in a cell type-dependent manner...
August 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27171904/current-knowledge-of-vitamin-d-in-dogs
#18
Nicole Weidner, Adronie Verbrugghe
There is emerging interest in linking vitamin D status to physiological health and disease states in the dog, as evidenced by the recent increase in publications in this area. This research has most likely been spurred by the studies exploring vitamin D and disease in humans. However, there are important differences in vitamin D intake and metabolism between humans and dogs that should be accounted for. The understanding of basic vitamin D metabolism and the relationship between vitamin D intake and vitamin D status in dogs remains even more limited than current knowledge in humans...
May 12, 2016: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/27153102/analytic-validation-of-commercially-available-immunoassays-for-the-measurement-of-serum-cobalamin-and-folate-concentrations-in-pigs
#19
Niels Grützner, Darrel Knabe, Bruce D Lawhorn, Brandon Dominguez, Johannes Kauffold, Jan S Suchodolski, Jörg M Steiner
BACKGROUND: Cobalamin (vitamin B12 ) and folate (vitamin B9 ) are important for the amino acid metabolism and nucleic acid synthesis. Immunoassays for the measurement of both vitamins in serum are routinely used in people, cats, and dogs, serving as indicators for clinical disorders including cobalamin and/or folate deficiency, small intestinal dysbiosis, or inadequate dietary supply of these vitamins. The analysis of these analytes may also be clinically useful in pigs. OBJECTIVE: The purpose of this study was to analytically validate immunoassays for the measurement of cobalamin and folate concentrations in porcine serum, and to determine serum cobalamin and folate concentrations in healthy newborn pigs pre- and postweaning...
June 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27033937/plasma-biomarkers-of-sirs-and-mods-associated-with-canine-babesiosis
#20
J Kuleš, C de Torre-Minguela, R Barić Rafaj, J Gotić, P Nižić, J J Ceron, V Mrljak
Canine babesiosis is a tick-borne disease caused by the haemoprotozoan parasites of the genus Babesia. Early detection of systemic inflammatory response syndrome (SIRS) and multiple organ dysfunction syndrome (MODS) is of major importance in clinical practice for providing information about severity and outcomes of the disease and therapy. Plasma samples were taken at admission from five dogs with uncomplicated babesiosis caused by B. canis canis, five dogs with babesiosis and SIRS, five dogs with babesiosis and MODS, and five healthy dogs...
April 2016: Research in Veterinary Science
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