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vitamin D Receptor Gene HIV

Wbeimar Aguilar-Jimenez, Simon Villegas-Ospina, Sandra Gonzalez, Wildeman Zapata, Irma Saulle, Micaela Garziano, Mara Biasin, Mario Clerici, Maria T Rugeles
BACKGROUND: Although the anti-HIV-1 effects of Vitamin D (VitD) have been reported, mechanisms behind such protection remain largely unexplored. METHODS: Effects of active VitD (calcitriol) at 10, 10 and 10 M or its precursor (calcidiol) at 100 and 250 nM, on HIV-1 infection, immune activation and gene expression were analyzed in vitro in cells of Colombian and Italian healthy donors, respectively. We quantified levels of released p24 by ELISA, of intracellular p24 and cell-surface expression of CD38 and HLA-DR by flow cytometry, and mRNA expression of antiviral and immunoregulatory genes by qRT-PCR...
August 5, 2016: Journal of Acquired Immune Deficiency Syndromes: JAIDS
Lynne McNamara, Simbarashe Takuva, Tobias Chirwa, Patrick MacPhail
BACKGROUND: Host genetic factors may a play role in susceptibility to infection. Vitamin-D is an immunomodulator that may play a role in HIV infection. Vitamin-D action is mediated by the vitamin-D receptor. We establish prevalence of ApaI, BsmI, FokI and TaqI polymorphisms (VDRPs) amongst a black southern African HIV+ve population and investigate polymorphic differences between HIV+ve and -ve people. METHODS: Seventy-nine sex and age-group matched HIV+ve patients of African origin initiating antiretroviral therapy (ART) and 79 HIV-ve participants, also of African origin, were recruited from a public sector HIV testing and treatment clinic and investigated for the 4 polymorphisms...
2016: International Journal of Molecular Epidemiology and Genetics
Amita Gupta, Grace Montepiedra, Akshay Gupte, Bret Zeldow, Jennifer Jubulis, Barbara Detrick, Avy Violari, Shabir Madhi, Raziya Bobat, Mark Cotton, Charles Mitchell, Stephen Spector
BACKGROUND: This study examined the associations of 25-hydroxyvitamin D and specific host genetic variants that affect vitamin D levels or its effects on immune function, with the risk of TB or mortality in children. METHODS: A case-cohort sample of 466 South African infants enrolled in P1041 trial (NCT00080119) underwent 25-hydroxyvitamin D testing by chemiluminescent immunoassay. Single nucleotide polymorphisms (SNPs) that alter the effect of vitamin D [e.g. vitamin D receptor (VDR)], vitamin D levels [e...
2016: PloS One
Liling Huang, Cunxu Liu, Guangfu Liao, Xiaobing Yang, Xiuwen Tang, Jingjie Chen
The association between vitamin D receptor (VDR) FokI polymorphism and tuberculosis (TB) risk remains a matter of debate. Potential selection bias exists in most studies using HIV-positive TB patients.An update meta-analysis was carried out to derive a more reliable assessment of the association between FokI polymorphisms and TB risk, especially in HIV-negative TB patients. All major databases from inception to June 2015 were searched for all publications that studied the association between FokI polymorphism and TB risk...
December 2015: Medicine (Baltimore)
Chun Xu, Peijun Tang, Cheng Ding, Chang Li, Jun Chen, Zhenlei Xu, Yi Mao, Meiying Wu, Jun Zhao
BACKGROUND: Vitamin D receptor (VDR) gene FokI polymorphism have been studied in relation to tuberculosis (TB) in many populations and provided inconsistent results. In this study, we carried out a meta-analysis to derive a more reliable assessment on FokI polymorphism and the risk of HIV-negative TB. METHODS: The Embase, PubMed, and Cochrane Library databases were used to undertake a comprehensive systematic literature review of all current published VDR gene FOKI association studies aimed at the risk of TB up to June 30, 2015...
2015: PloS One
Nirupama Chandel, Ashwani Malhotra, Pravin C Singhal
Illicit drug abuse is highly prevalent and serves as a powerful co-factor for HIV exacerbation. Epigenetic alterations in drug abuse and HIV infection determine expression of several critical genes such as vitamin D receptor (VDR), which participates in proliferation, differentiation, cell death under both physiological and pathological conditions. On that account, active vitamin D, the ligand of VDR, is used as an adjuvant therapy to control infection, slow down progression of chronic kidney diseases, and cancer chemotherapy...
2015: Frontiers in Microbiology
Nirupama Chandel, Kameshwar S Ayasolla, Xiqian Lan, Maria Sultana-Syed, Amrita Chawla, Rivka Lederman, Vasupradha Vethantham, Moin A Saleem, Praveen N Chander, Ashwani Malhotra, Pravin C Singhal
HIV (human immunodeficiency virus) has been reported to induce podocyte injury through down regulation of vitamin D receptor (VDR) and activation of renin angiotensin system; however, the involved mechanism is not clear. Since HIV has been reported to modulate gene expression via epigenetic phenomena, we asked whether epigenetic factors contribute to down regulation of VDR. Kidney cells in HIV transgenic mice and HIV-infected podocytes (HIV/HPs) displayed enhanced expression of SNAIL, a repressor of VDR. To elucidate the mechanism, we studied the effect of HIV on expression of molecules involved in SNAIL repressor complex formation and demonstrated that HIV enhances expression of the histone deacetylase HDAC1 and DNA methyl transferases DNMT3b and DNMT1...
October 9, 2015: Journal of Molecular Biology
Partab Rai, Tejinder Singh, Rivka Lederman, Amrita Chawla, Dileep Kumar, Kang Cheng, Gautam Valecha, Peter W Mathieson, Moin A Saleem, Ashwani Malhotra, Pravin C Singhal
In the present study, we evaluated the effect of short term hyperglycemia on renal lesions in a mouse model (Tg26) of HIV-associated nephropathy (HIVAN). Control and Tg26 mice in groups (n=6) were administered either normal saline (FVBN or Tg) or streptozotocin (FVBN+STZ or Tg26+STZ). After two weeks, biomarkers were collected and kidneys were harvested. FVBN+ STZ and Tg26+STZ displayed elevated serum glucose levels when compared to FVBN and Tg26 respectively. Tg26+STZ displayed elevated (P<0.05) blood urea nitrogen (BUN) levels (P<0...
March 2015: Cellular Signalling
Marina Laplana, Manuel Sánchez-de-la-Torre, Teresa Puig, Antonio Caruz, Joan Fibla
Vitamin-D has pleiotropic effects on calcium and bone metabolism, cellular growth control, cell differentiation and modulation of both innate and acquired immune response. Previous studies revealed the association of vitamin-D receptor gene (VDR) polymorphism with infection diseases including HIV-1 infection. To assess for association between polymorphisms of vitamin-D pathway genes CYP27B1, vitamin-D binding protein (VDBP) and VDR with HIV-1 infection, disease progression to acquired immunodeficiency syndrome (AIDS) was analysed according to CDC93 criteria in a cohort of 185 HIV-1 seroprevalent patients belonging to the injection drug users...
July 15, 2014: Gene
Nirupama Chandel, Bipin Sharma, Mohammad Husain, Divya Salhan, Tejinder Singh, Partab Rai, Peter W Mathieson, Moin A Saleem, Ashwani Malhotra, Pravin C Singhal
Alterations in the podocyte actin cytoskeleton have been implicated in the development of proteinuric kidney diseases. In the present study, we evaluated the effect of HIV on the podocyte actin cytoskeleton and the mechanism involved. We hypothesized that HIV may be compromising the actin cytoskeleton via downregulation of the vitamin D receptor (VDR) of conditionally immortalized differentiated human podocytes (CIDHPs). HIV-transduced podocytes (HIV/CIDHPs) not only displayed downregulation of VDR but also showed activation of the renin-angiotensin system (RAS) in the form of enhanced expression of renin and increased production of ANG II...
June 1, 2013: American Journal of Physiology. Renal Physiology
Khanh vinh quoc Luong, Lan Thi Hoàng Nguyen
Patients with human immuno-deficiency virus (HIV)-infection have a high prevalence of abnormal bone metabolism and vitamin D deficiency. Vitamin D treatment has some benefit in patients with HIV infection. In this paper, we review the evidence for an association between vitamin D and HIV infection. Literature search was done from Medline. Genetic studies have provided the opportunity to determine which proteins link vitamin D to HIV pathology [i.e., the major histocompatibility complex class II molecules, vitamin D receptor, cytochrome P(450), renin-angiotensin system, apoli-poprotein E, liver X receptor, toll-like receptor, poly(ADP-ribosyl) polymerase-1, natural resistance-associated macrophage protein 1, and the Sp1 promoter gene]...
January 2013: Current HIV Research
Ai-Leng Khoo, Hans J P M Koenen, Meta Michels, Sharon Ooms, Marjolein Bosch, Mihai G Netea, Irma Joosten, André J A M van der Ven
Vitamin D(3) is known to have an effect on the immune function. We investigated the immunomodulatory capability of vitamin D(3) in HIV-infected patients and studied the expression of chemokine receptors on regulatory T cells (Treg). Vitamin D(3)-deficient HIV-1-seropositive subjects were treated with cholecalciferol (vitamin D(3)) at a dose of 800 IU daily for 3 months (n=9) or 25,000 IU weekly for 2 months (n=7). Peripheral blood mononuclear cells (PBMCs) were isolated and analyzed for skin-homing (CCR4 and CCR10) and gut-homing (CCR9 and integrin α(4)β(7)) marker expression on Treg, by flow cytometry, before and after supplementation...
February 2013: AIDS Research and Human Retroviruses
Divya Salhan, Mohammad Husain, Ashaan Subrati, Rohan Goyal, Tejinder Singh, Partab Rai, Ashwani Malhotra, Pravin C Singhal
Reactive oxygen species (ROS) have been demonstrated to contribute to HIV-induced tubular cell injury. We hypothesized that HIV-induced ROS generation may be causing tubular cell injury through downregulation of vitamin D receptor (VDR) and associated downstream effects. In the present study, HIV not only downregulated tubular cell VDR expression but also inflicted DNA injury. On the other hand, EB-1089, a VDR agonist (VD), inhibited both downregulation of VDR and tubular cell DNA injury in the HIV milieu. H(2)O(2) (an O(-) donor) directly downregulated tubular cell VDR, whereas catalase, a free radical scavenger, inhibited HIV-induced downregulation of tubular cell VDR expression...
August 15, 2012: American Journal of Physiology. Renal Physiology
S Raghavan, K Alagarasu, P Selvaraj
Tuberculosis (TB) is the frequent major opportunistic infection in HIV-infected patients, and is the leading cause of mortality among HIV-infected patients. Genetic susceptibility to TB in HIV negative subjects is well documented. Since coinfections can influence the way in which immune system respond to different pathogens, genetic susceptibility to TB in HIV patients might also change. Studies from India and other parts of the world have shown that genetic susceptibility to TB is influenced by HIV infection...
January 2012: Tuberculosis
Leah A Garcia, Keisha K King, Monica G Ferrini, Keith C Norris, Jorge N Artaza
Skeletal muscle wasting is an important public health problem associated with aging, chronic disease, cancer, kidney dialysis, and HIV/AIDS. 1,25-Dihydroxyvitamin D (1,25-D3), the active form of vitamin D, is widely recognized for its regulation of calcium and phosphate homeostasis in relation to bone development and maintenance and for its calcemic effects on target organs, such as intestine, kidney, and parathyroid glands. Emerging evidence has shown that vitamin D administration improves muscle performance and reduces falls in vitamin D-deficient older adults...
August 2011: Endocrinology
P Selvaraj
Vitamin D plays a major role in bone mineral density and calcium homeostasis. Apart from its classical action, the active form of vitamin D [1,25-dihydroxyvitamin D(3) (1,25(OH)(2)D(3))] influences the innate and adaptive immune functions through vitamin D receptor (VDR) that are present in various cells of the immune system. Vitamin D deficiencies have been associated with development of tuberculosis (TB) disease, caused by Mycobacterium tuberculosis. Vitamin D(3) is shown to enhance macrophage phagocytosis of M...
2011: Vitamins and Hormones
Carmen Torres, Manuel Sánchez de la Torre, Carelia García-Moruja, Antonio José Carrero, María del Monte Trujillo, Joan Fibla, Antonio Caruz
Vitamin-D-receptor (VDR) mediates immunomodulatory effects of vitamin-D₃ (VD₃). The VDR-rs1544410_GG polymorphism has been associated with delayed progression rates to AIDS and resistance to HIV-1 infection. The aim of the present study was to investigate differences in VD₃ mediated effects on rs1544410 genotyped dendritic cells (DCs) and macrophages (MDM), key cells involved in HIV-1 infection. Immature DCs exhibited lower b-actin-normalized VDR mRNA expression in rs1544410_GG compared to cells with a rs1544410_AA genotype...
September 2010: Current HIV Research
Inna G Ovsyannikova, Neelam Dhiman, Iana H Haralambieva, Robert A Vierkant, Megan M O'Byrne, Robert M Jacobson, Gregory A Poland
Toll-like, vitamin A and D receptors and other innate proteins participate in various immune functions. We determined whether innate gene-sequence variations are associated with rubella vaccine-induced cytokine immune responses. We genotyped 714 healthy children (11-19 years of age) after two doses of rubella-containing vaccine for 148 candidate SNP markers. Rubella virus-induced cytokines were measured by ELISA. Twenty-two significant associations (range of P values 0.002-0.048) were found between SNPs in the vitamin A receptor family (RARA, RARB, TOP2B and RARG), vitamin D receptor and downstream mediator of vitamin D signaling (RXRA) genes and rubella virus-specific (IFN-gamma, IL-2, IL-10, TNF-alpha, and GM-CSF) cytokine immune responses...
February 2010: Human Genetics
Oznur Tastan, Yanjun Qi, Jaime G Carbonell, Judith Klein-Seetharaman
Human immunodeficiency virus-1 (HIV-1) in acquired immune deficiency syndrome (AIDS) relies on human host cell proteins in virtually every aspect of its life cycle. Knowledge of the set of interacting human and viral proteins would greatly contribute to our understanding of the mechanisms of infection and subsequently to the design of new therapeutic approaches. This work is the first attempt to predict the global set of interactions between HIV-1 and human host cellular proteins. We propose a supervised learning framework, where multiple information data sources are utilized, including co-occurrence of functional motifs and their interaction domains and protein classes, gene ontology annotations, posttranslational modifications, tissue distributions and gene expression profiles, topological properties of the human protein in the interaction network and the similarity of HIV-1 proteins to human proteins' known binding partners...
2009: Pacific Symposium on Biocomputing
K Alagarasu, P Selvaraj, S Swaminathan, G Narendran, P R Narayanan
INTRODUCTION: Vitamin D receptor (VDR) gene polymorphisms in the 5' regulatory region (Cdx2 and A-1012G), coding region (FokI), and 3' untranslated region (UTR; BsmI, ApaI, and TaqI) were studied to find out whether these polymorphisms are associated with susceptibility to or protection against HIV-1 and development of tuberculosis (TB) in human immunodeficiency virus (HIV)-1-infected patients. STUDY SUBJECTS AND METHODS: The study was carried out in 131 HIV patients without TB (HIV+ TB-) and 113 HIV patients with TB (HIV+ TB+; includes 82 patients with pulmonary TB (HIV+ PTB+) and 31 with extra pulmonary TB), 108 HIV-negative pulmonary TB patients (HIV- PTB+), and 146 healthy controls...
March 2009: Journal of Clinical Immunology
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