keyword
MENU ▼
Read by QxMD icon Read
search

FokI

keyword
https://www.readbyqxmd.com/read/29643436/satellite-dna-in-vicia-faba-is-characterized-by-remarkable-diversity-in-its-sequence-composition-association-with-centromeres-and-replication-timing
#1
Laura Ávila Robledillo, Andrea Koblížková, Petr Novák, Katharina Böttinger, Iva Vrbová, Pavel Neumann, Ingo Schubert, Jiří Macas
Satellite DNA, a class of repetitive sequences forming long arrays of tandemly repeated units, represents substantial portions of many plant genomes yet remains poorly characterized due to various methodological obstacles. Here we show that the genome of the field bean (Vicia faba, 2n = 12), a long-established model for cytogenetic studies in plants, contains a diverse set of satellite repeats, most of which remained concealed until their present investigation. Using next-generation sequencing combined with novel bioinformatics tools, we reconstructed consensus sequences of 23 novel satellite repeats representing 0...
April 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29589202/the-impact-of-five-vdr-polymorphisms-on-multiple-sclerosis-risk-and-progression-a-case-control-and-genotype-phenotype-study
#2
Pavel Křenek, Yvonne Benešová, Julie Bienertová-Vašků, Anna Vašků
Vitamin D receptor polymorphisms have been the target of many studies focusing on multiple sclerosis. However, previously reported results have been inconclusive. The objective of this study was to investigate the association between five vitamin D receptor polymorphisms (EcoRV, FokI, ApaI, TaqI, and BsmI) and multiple sclerosis susceptibility and its course. The study was carried out as a case-control and genotype-phenotype study, consisted of 296 Czech multiple sclerosis patients and 135 healthy controls...
March 28, 2018: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29581796/polymorphisms-in-vitamin-d-receptor-genes-in-association-with-childhood-autism-spectrum-disorder
#3
Zengyu Zhang, Sufang Li, Lianfang Yu, Jun Liu
Both genetic and environmental factors have been implicated in the etiology of autism spectrum disorder (ASD). This case-control study aimed to determine the association of single-nucleotide polymorphisms (SNPs) rs731276 (TaqI), rs1568820 (Cdx2), rs1544410 (BsmI), and rs2228570 (FokI) in the vitamin D receptor (VDR) gene with susceptibility of childhood ASD and severity of the disease. A total of 201 children with ASD and 200 healthy controls from the Han Chinese population were recruited. SNP genotyping was carried out by TaqMan probe-based real-time PCR using genomic DNA extracted from blood cells...
2018: Disease Markers
https://www.readbyqxmd.com/read/29569696/association-of-rs2228570-polymorphism-of-vitamin-d-receptor-gene-with-lumbar-degenerative-disc-disease
#4
Selçuk Özdoğan, Cumhur Kaan Yaltirik, Seda Güleç Yilmaz, Ayhan Koçak, Turgay Isbir
AIM: Lumbar degenerative disc disease (LDDD) is the most common cause of lower back pain (LBP) and sciatica. The vitamin D receptor (VDR) gene, which is located on chromosome 12 (12q12-q14), was the first gene reported to be potentially related to intervertebral degenerative disc disease risks. We conducted a case-control study of a Turkish population and investigated the association between the VDR gene rs2228570 FokI polymorphism and the development of LDDD. MATERIAL AND METHODS: This was a prospective case-control study that included 45 patients with LDDD and 49 healthy individuals (control group)...
March 11, 2018: Turkish Neurosurgery
https://www.readbyqxmd.com/read/29562138/rational-design-of-mini-cas9-for-transcriptional-activation
#5
Dacheng Ma, Shuguang Peng, Weiren Huang, Zhiming Cai, Zhen Xie
Nuclease dead Cas9 (dCas9) has been widely used for modulating gene expression by fusing with different activation or repression domains. However, delivery of the CRISPR/Cas system fused with variant effector domains in a single adeno-associated virus (AAV) remains challenging due to the payload limit. Here, we engineered a set of downsized variants of Cas9 including Staphylococcus aureus Cas9 (SaCas9) that retained DNA binding activity by deleting conserved functional domains. We demonstrated that fusing FokI nuclease domain to the N-terminal of the minimal SaCas9 (mini-SaCas9) or to the middle of the split mini-SaCas9 can trigger efficient DNA cleavage...
March 21, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29560132/associations-between-vdr-gene-polymorphisms-and-colorectal-cancer-susceptibility-an-updated-meta-analysis-based-on-39-case-control-studies
#6
Zhipeng Pan, Mengya Chen, Xingxing Hu, Hua Wang, Jiajia Yang, Congjun Zhang, Faming Pan, Guoping Sun
Background: Recent studies have reported the associations between vitamin D receptor ( VDR ) polymorphisms and colorectal cancer (CRC), but the results were not always consistent. This meta-analysis aims to evaluate whether VDR polymorphisms are associated with CRC susceptibility. Materials And Methods: Studies on the associations between VDR polymorphisms and CRC were retrieved from the Web of Science, PubMed, the Chinese Biomedical Database (CBM), Chinese National Knowledge Infrastructure (CNKI) and Wanfang (Chinese) databases...
February 27, 2018: Oncotarget
https://www.readbyqxmd.com/read/29555202/association-of-vitamin-d-receptor-gene-polymorphisms-with-serum-25-oh-d-levels-and-metabolic-syndrome-in-thai-population
#7
Boonnisa Sangkaew, Manit Nuinoon, Nutjaree Jeenduang
Metabolic syndrome (MetS) increases the risk of developing cardiovascular disease (CVD) and type 2 diabetes mellitus (T2DM). The vitamin D receptor gene (VDR) polymorphisms have been found associated with MetS and serum 25(OH)D levels but these associations remain controversial. The aim of this study was to investigate the relationship between the VDR polymorphisms and MetS, metabolic components, and serum 25(OH)D levels within the Thai population. A case-control study included 237 participants with MetS according to the MetS diagnostic criteria of NCEP ATPIII and 376 controls...
March 16, 2018: Gene
https://www.readbyqxmd.com/read/29544394/association-of-vitamin-d-receptor-gene-taqi-foki-and-apai-variants-with-arteriovenous-fistula-failure-in-hemodialysis-patients
#8
Can Huzmeli, Gokhan Bagci, Ferhan Candan, Binnur Bagci, Lale Akkaya, Mansur Kayatas
PURPOSE: We investigated the influence of the vitamin D receptor gene TaqI (rs731236), ApaI (rs7975232), and FokI (rs2228570) polymorphisms in arteriovenous fistula failure in hemodialysis patients. METHODS: This study was carried out with 54 patients who experienced two or more fistula failures in the late period after arteriovenous fistula operation and 58 control patients with no history of arteriovenous fistula failure in 3 years or longer. The polymerase chain reaction-restriction fragment length polymorphism method was used to determine the vitamin D receptor TaqI, FokI, and ApaI polymorphisms...
March 1, 2018: Journal of Vascular Access
https://www.readbyqxmd.com/read/29535654/using-cognitive-agents-to-train-negotiation-skills
#9
Christopher A Stevens, Jeroen Daamen, Emma Gaudrain, Tom Renkema, Jakob Dirk Top, Fokie Cnossen, Niels A Taatgen
Training negotiation is difficult because it is a complex, dynamic activity that involves multiple parties. It is often not clear how to create situations in which students can practice negotiation or how to measure students' progress. Some have begun to address these issues by creating artificial software agents with which students can train. These agents have the advantage that they can be "reset," and played against multiple times. This allows students to learn from their mistakes and try different strategies...
2018: Frontiers in Psychology
https://www.readbyqxmd.com/read/29530503/vitamin-d-deficiency-and-vitamin-d-receptor-variants-in-mothers-and-their-neonates-are-risk-factors-for-neonatal-sepsis
#10
Safaa I Tayel, Shimaa E Soliman, Hanan M Elsayed
BACKGROUND AND AIM: increasing prevalence of neonatal sepsis in recent years catch attention to early prevention and management. Vitamin D receptor (VDR) polymorphism can modulate VDR expression level that greatly influences immunity and susceptibility to microbial infections. We aimed to investigate the association of VDR polymorphism at FokI, rs2228570 T/C, and TaqI, rs731236 C/T gene with serum 25-hydroxyvitamin D level and risk of neonatal sepsis. METHODS: This work carried on 160 subjects classified into 80 cases (40 mothers and their 40 septic neonates) and 80 healthy controls (40 volunteer mothers and their 40 healthy neonates)...
March 9, 2018: Steroids
https://www.readbyqxmd.com/read/29506625/vdbp-vdr-mutations-and-other-factors-related-with-vitamin-d-metabolism-may-be-associated-with-type-1-diabetes-mellitus
#11
Deniz Kirac, Ceyda Dincer Yazan, Hazal Gezmis, Ali Yaman, Goncagul Haklar, Onder Sirikci, Elif Cigdem Altunok, Oguzhan Deyneli
Type 1 diabetes mellitus (T1DM) is an insulin dependent autoimmune disorder resulting the progressive destruction of pancreatic beta cells. Another possible factor considered to be related with T1DM is vitamin D deficiency. Therefore in this study it was aimed to investigate the associations between T1DM, vitamin D binding protein (VDBP) and vitamin D receptor (VDR) gene mutations which are related with vitamin D metabolism. Fifty five T1DM paitents and 40 healthy volunteers were recruited to the study. FokI (rs2228570), BsmI (rs1544410) mutations in VDR; rs4588 and rs7041 polymorphisms in VDBP were investigated with real-time polymerase chain reaction (RT-PCR)...
February 28, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29483716/-influence-of-vitamin-d-receptor-foki-polymorphism-on-expression-of-cyp24a1-in-periodontal-cells
#12
K N Liu, H X Meng, J X Hou
OBJECTIVE: There is asingle nucleotide polymorphism (SNP) in the exon 2 of the vitamin D receptor (VDR) gene that can be distinguished using the restriction endonuclease FokI, and accordingly divided into three genotypes: FF, Ff and ff. VDR-FokI polymorphism was the only known SNP that could alter the protein structure of VDR. CYP24A1 is the gene encoding vitamin D 24 hydroxylase and is a vitamin D responsive gene. The influence of rs2228570 on transcriptional activation by VDR in human gingival fibroblasts (hGF) and periodontal ligament cells (hPDLC) was investigated in this study...
February 18, 2018: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/29479872/associations-of-vitamin-d-levels-and-vitamin-d-receptor-genotypes-with-patient-reported-outcome-disease-activity-in-patients-with-rheumatoid-arthritis
#13
Sawsan O Khoja, Yasser El-Miedany, Archana P Iyer, Sami M Bahlas, Khadijah S Balamash, Mohamed F Elshal
BACKGROUND: The aim of this study is to evaluate the prevalence of vitamin-D insufficiency and vitamin-D receptor (VDR) polymorphisms in rheumatoid arthritis (RA) patients and its association with disease activity and patient reported outcomes (PROs). METHODS: Eighty-two individuals were included in a cross-sectional study (41 RA patients, 41 controls). Prior to assessment, each patient completed a PRO questionnaire. Serum vitamin-D levels and genotyping for VDR were assessed...
January 1, 2018: Clinical Laboratory
https://www.readbyqxmd.com/read/29468338/vitamin-d-receptor-foki-taqi-and-apai-polymorphisms-and-susceptibility-to-systemic-lupus-erythematosus-an-updated-meta-analysis
#14
Sang-Cheol Bae, Young Ho Lee
This study aimed to explore whether vitamin D receptor (VDR) polymorphisms are associated with susceptibility to systemic lupus erythematosus (SLE). Meta-analyses were conducted to determine the associations between the VDR FokI, TaqI, and ApaI polymorphisms and SLE in all subjects and each ethnic group. A total of 12 studies were considered in the meta-analysis, which involved 1974 patients and 2506 controls. Meta-analysis of the VDR FokI polymorphism showed no association between SLE and the FokI F allele in all study subjects (OR = 1...
February 21, 2018: Clinical Rheumatology
https://www.readbyqxmd.com/read/29467039/influence-of-lifestyle-characteristics-and-vdr-polymorphisms-as-risk-factors-for-intervertebral-disc-degeneration-a-case-control-study
#15
Luiz Angelo Vieira, Aline Amaro Dos Santos, Carla Peluso, Caio Parente Barbosa, Bianca Bianco, Luciano Miller Reis Rodrigues
BACKGROUND: Intervertebral disc degeneration (DD) is an important cause of low back pain and its precise aetiology is not fully understood, being attributed to cumulative environmental, biomechanical and genetic effects. The vitamin D plays a key role in regulation of calcium homeostasis and bone mineralization, exerting its biological activities by binding to a high-affinity receptor (VDR). Polymorphisms in VDR gene were previously associated with DD process, however with conflicting results...
February 21, 2018: European Journal of Medical Research
https://www.readbyqxmd.com/read/29465575/impact-of-vitamin-d-receptor-and-binding-protein-gene-polymorphisms-in-clinical-and-laboratory-data-of-hcv-patients-cross-sectional-study
#16
Letícia de Paula Scalioni, Betânia Rodrigues Dos Santos, Poli Mara Spritzer, Cristiane Alves Villela-Nogueira, Lia Laura Lewis-Ximenez, Priscila Pollo-Flores, Eliane Bordalo Cathalá Esberard, Carlos Eduardo Brandão-Mello, Elisabeth Lampe, Livia Melo Villar
Potential relationship of vitamin D, vitamin D receptor (VDR), and vitamin D binding protein (DBP) have been suggested in the pathophysiology of hepatitis C virus (HCV) infection. The aim of this observational study is to determine vitamin D levels, and VDR and DBP genetic polymorphism according demographic and laboratory data in chronic HCV patients (CHC).A total of 148 CHC patients gave serum samples for testing 25-hydroxyvitamin D (25 (OH)D) level by immunochemiluminometric assay (<20 ng/mL defined as deficient) and donated blood samples to allelic discrimination analysis using TaqMan assays...
February 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29458003/fgf-23-pth-and-vitamin-d-status-in-end-stage-renal-disease-patients-affected-by-vdr-foki-and-bsmi-variants
#17
Mouna Bouksila, Wajih Kaabachi, Mehdi Mrad, Wided Smaoui, Elhem Cheour El Kateb, Mohammed Karim Zouaghi, Kamel Hamzaoui, Afef Bahlous
OBJECTIVES: The aim of this study was to evaluate the association between two VDR SNPs FokI and BsmI and mineral status in ESRD patients. DESIGN AND METHODS: Our case-control study included 100 patients with chronic renal failure in ESRD and 149 healthy subjects. We measured the serum Vitamin D levels and the serum intact PTH level by Electrochemiluminescence Technology (cobas E411 analyzer). We evaluated the serum FGF23 levels by indirect ELISA method. The genotyping of two VDR gene variants FokI and BsmI was carried out by PCR-RFLP technique...
February 16, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29397342/high-frequency-of-type-2-diabetes-and-impaired-glucose-tolerance-in-japanese-subjects-with-the-angiopoietin-like-protein-8-r59w-variant
#18
Jianhui Liu, Kunimasa Yagi, Atsushi Nohara, Daisuke Chujo, Azusa Ohbatake, Aya Fujimoto, Yukiko Miyamoto, Junji Kobayashi, Masakazu Yamagishi
BACKGROUND: Angiopoietin-like protein 8 (ANGPTL8) is considered to be metabolically multifunctional. One notable function still to be elucidated definitively is a betatrophic role in protecting and preserving pancreatic beta-cell function. There is, however, a paucity of data regarding the role of ANGPTL8 in the etiology of type 2 diabetes (T2D), but some findings of human research have suggested the potential for significant involvement. OBJECTIVE: To examine the frequency of T2D and impaired glucose tolerance (IGT) in Japanese subjects with the ANGPTL8 R59W variant...
December 29, 2017: Journal of Clinical Lipidology
https://www.readbyqxmd.com/read/29391820/relationship-of-vitamin-d-receptor-gene-polymorphisms-in-helicobacter-pylori-gastric-patients
#19
Dinelma de Jesus Martins, Gyselly Cb Matos, Rosane Sp Loiola, Vivian D'Annibale, Tereza Corvelo
Purpose: The present study aimed to investigate the association between the human VDR gene and Helicobacter pylori infection. Patients and methods: A cross-sectional study was conducted on 208 adult patients with upper gastrointestinal symptoms. Gastric biopsy specimens were obtained from each patient for molecular DNA and histological examination. Patients were genotyped for VDR gene polymorphisms using polymerase chain reaction and restriction fragment length polymorphism analysis...
2018: Clinical and Experimental Gastroenterology
https://www.readbyqxmd.com/read/29388852/associations-between-vitamin-d-receptor-polymorphisms-and-susceptibility-to-behcet-s-disease-a-meta-analysis
#20
Zahra Mirfeizi, Samira Tabaei, Yalda Ravanshad, Kamila Hashemzadeh, Elahe Kharazmi, Hassan Mehrad-Majd
BACKGROUND: The vitamin D receptor (VDR) gene polymorphisms have been reported to be related to the development of Behcet's disease (BD). However, the results have been inconsistent among diverse populations. Therefore, this comprehensive meta-analysis has been designed to assess a more accurate association between VDR polymorphisms and BD susceptibility. METHODS: An electronic literature search was conducted to identify eligible studies. Pooled odds ratios (OR) with corresponding 95% confidence interval (CI) were calculated in different genetic models to assess this association...
February 1, 2018: Immunological Investigations
keyword
keyword
103910
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"