keyword
MENU ▼
Read by QxMD icon Read
search

FokI

keyword
https://www.readbyqxmd.com/read/27906437/vitamin-d-receptor-polymorphisms-as-tool-for-early-screening-of-severe-bone-loss-in-women-patients-with-rheumatoid-arthritis
#1
G Di Spigna, A Del Puente, B Covelli, E Abete, E Varriale, S Salzano, L Postiglione
OBJECTIVE: Rheumatoid Arthritis (RA) is an autoimmune inflammatory disease that leads to local and systemic arthritis and bone loss. Exploring genetic markers of candidate genes in osteoporosis and inflammatory cytokine genes could be a useful tool for the early identification of bone loss and fracture risk in RA patients. The target of this study is the evaluation and correlation between of Single Nucleotide Polymorphisms (SNPs) of Vitamin D Receptor (VDR) and possible effects on bone loss in RA...
November 2016: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/27905063/efficient-gene-targeting-in-mouse-zygotes-mediated-by-crispr-cas9-protein
#2
Chris J Jung, Junli Zhang, Elizabeth Trenchard, Kent C Lloyd, David B West, Barry Rosen, Pieter J de Jong
The CRISPR/Cas9 system has rapidly advanced targeted genome editing technologies. However, its efficiency in targeting with constructs in mouse zygotes via homology directed repair (HDR) remains low. Here, we systematically explored optimal parameters for targeting constructs in mouse zygotes via HDR using mouse embryonic stem cells as a model system. We characterized several parameters, including single guide RNA cleavage activity and the length and symmetry of homology arms in the construct, and we compared the targeting efficiency between Cas9, Cas9nickase, and dCas9-FokI...
November 30, 2016: Transgenic Research
https://www.readbyqxmd.com/read/27899266/dna-aptamers-against-foki-nuclease-domain-for-genome-editing-applications
#3
Maui Nishio, Daisuke Matsumoto, Yoshio Kato, Koichi Abe, Jinhee Lee, Kaori Tsukakoshi, Ayana Yamagishi, Chikashi Nakamura, Kazunori Ikebukuro
Genome editing with site-specific nucleases (SSNs) can modify only the target gene and may be effective for gene therapy. The main limitation of genome editing for clinical use is off-target effects; excess SSNs in the cells and their longevity can contribute to off-target effects. Therefore, a controlled delivery system for SSNs is necessary. FokI nuclease domain (FokI) is a common DNA cleavage domain in zinc finger nuclease (ZFN) and transcription activator-like effector nuclease. Previously, we reported a zinc finger protein delivery system that combined aptamer-fused, double-strand oligonucleotides and nanoneedles...
November 22, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27891515/the-association-between-vdr-gene-polymorphisms-and-diabetic-retinopathy-susceptibility-a-systematic-review-and-meta-analysis
#4
Yun Zhang, Wei Xia, Ping Lu, Huijuan Yuan
Aims. Studies on the associations of vitamin D receptor (VDR) gene polymorphisms with diabetic retinopathy (DR) susceptibility reported conflicting results. A systematic meta-analysis was undertaken to clarify this topic. Methods. A systematic search of electronic databases (PubMed, EMBASE, and CNKI) was carried out until March 31, 2016. The pooled odds ratio (OR) and 95% confidence interval (CI) were used to assess the strength of the association. Results. A total of 7 studies fulfilling the inclusion criteria were included in this meta-analysis (649 cases and 707 controls)...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27883072/non-rvd-mutations-that-enhance-the-dynamics-of-the-tal-repeat-array-along-the-superhelical-axis-improve-talen-genome-editing-efficacy
#5
Naoya Tochio, Kohei Umehara, Jun-Ichi Uewaki, Holger Flechsig, Masaharu Kondo, Takehisa Dewa, Tetsushi Sakuma, Takashi Yamamoto, Takashi Saitoh, Yuichi Togashi, Shin-Ichi Tate
Transcription activator-like effector (TALE) nuclease (TALEN) is widely used as a tool in genome editing. The DNA binding part of TALEN consists of a tandem array of TAL-repeats that form a right-handed superhelix. Each TAL-repeat recognises a specific base by the repeat variable diresidue (RVD) at positions 12 and 13. TALEN comprising the TAL-repeats with periodic mutations to residues at positions 4 and 32 (non-RVD sites) in each repeat (VT-TALE) exhibits increased efficacy in genome editing compared with a counterpart without the mutations (CT-TALE)...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27836663/vitamin-d-receptor-gene-foki-variant-in-diabetic-foot-ulcer-and-its-relation-with-oxidative-stress
#6
Negin Soroush, Mania Radfar, Armita Kakavand Hamidi, Mohammad Abdollahi, Mostafa Qorbani, Farideh Razi, Ensieh Nasli Esfahani, Mahsa M Amoli
PURPOSE: The patient's suffering and financial costs affiliated with Diabetic Foot Ulcer (DFU), as one of the most important complications of diabetes, are highly undesirable and this highlights the importance of preventive medicine about this disorder. Furthermore hyperglycemia causes generation of free radicals which leads to oxidative stress (OS). Hence, this study aims to examine the association between vitamin D receptor (VDR) gene FokI polymorphism and DFU in Iranian population and also its correlation with OS biomarkers...
November 9, 2016: Gene
https://www.readbyqxmd.com/read/27834325/lack-of-association-between-foki-polymorphism-in-vitamin-d-receptor-gene-vdr-type-2-diabetes-mellitus-in-the-tunisian-population
#7
Imen Mahjoubi, Amani Kallel, Mohamed Hédi Sbaï, Bochra Ftouhi, Meriam Ben Halima, Zeineb Jemaa, Moncef Feki, Hedia Slimane, Riadh Jemaa, Naziha Kaabachi
BACKGROUND & OBJECTIVES: The impact of several environmental and genetic factors on diabetes is well documented. Though the association between the vitamin D receptor (VDR) gene polymorphisms and type 2 diabetes mellitus (T2DM) has been analyzed in different ethnic groups, the results have been inconsistent. The aim of this study was to evaluate the possible association between VDR FokI polymorphism and genetic susceptibility to T2DM in Tunisian population. METHODS: A total of 439 unrelated patients with T2DM and 302 healthy controls were included in the study...
July 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27806471/variations-in-vitamin-d-receptor-gene-adiposity-phenotypes-and-metabolic-profile-in-overweight-women-with-hypovitaminosis-d
#8
Samira Ebrahimof, Hamid Zand, Sakineh Shab-Bidar, Marjan Zarif Yeganeh, Farzaneh Jahangiri, Katayoun Pourvali, Maryam Sadat Daneshpour, Mehdi Hedayati
BACKGROUND: The possible role of vitamin D receptor (VDR) genotypes in determining adiposity phenotypes and cardiovascular risk factors has been suggested previously. The aim of this study was to evaluate the associations of the VDR gene variants with adiposity phenotypes and metabolic profile in Iranian overweight women with hypovitaminos is D. METHODS: Three VDR variants including FokI (rs2228570), BsmI (rs1544410) and rs757343 were detected using PCR-sequencing...
June 30, 2016: Global Journal of Health Science
https://www.readbyqxmd.com/read/27806302/telomere-internal-double-strand-breaks-are-repaired-by-homologous-recombination-and-parp1-lig3-dependent-end-joining
#9
Ylli Doksani, Titia de Lange
Shelterin protects chromosome ends from the DNA damage response. Although the mechanism of telomere protection has been studied extensively, the fate of double-strand breaks (DSBs) inside telomeres is not known. Here, we report that telomere-internal FokI-induced DSBs activate ATM kinase-dependent signaling in S-phase but are well tolerated and repaired efficiently. Homologous recombination contributes to repair, leading to increased telomere length heterogeneity typical of the alternative lengthening of telomeres (ALT) pathway...
November 1, 2016: Cell Reports
https://www.readbyqxmd.com/read/27798234/nuclear-constriction-segregates-mobile-nuclear-proteins-away-from-chromatin
#10
Jerome Irianto, Charlotte R Pfeifer, Rachel R Bennett, Yuntao Xia, Irena L Ivanovska, Andrea J Liu, Roger A Greenberg, Dennis E Discher
As a cancer cell squeezes its nucleus through adjacent tissue, penetrates a basement membrane, or enters a small blood capillary, chromatin density and nuclear factors might be physically perturbed. Here, in cancer cell migration through rigid micropores and in passive pulling into micropipettes, local compaction of chromatin is observed coincident with depletion of mobile factors. Hetero/eu-chromatin has been previously estimated from molecular mobility measurements to occupy a volume fraction f that might be two-thirds of the nuclear volume, but based on the relative intensity of DNA and histones in several cancer cell lines drawn into narrow constrictions, f can easily increase locally to nearly 100%...
October 26, 2016: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/27797588/association-between-the-foki-and-apai-polymorphisms-in-the-vitamin-d-receptor-gene-and-intervertebral-disc-degeneration-a-systematic-review-and-meta-analysis
#11
Noel Pabalan, Lani Tabangay, Hamdi Jarjanazi, Luiz Angelo Vieira, Aline Amaro Dos Santos, Caio Parente Barbosa, Luciano Miller Reis Rodrigues, Bianca Bianco
BACKGROUND: Evidence supporting an association of intervertebral disc degeneration (DD) with polymorphisms of the vitamin D receptor (VDR) gene has been controversial. We perform a meta-analysis of these studies to determine if there was substantial evidence to support such an association between the VDR polymorphisms and DD. METHODS: PubMed, Embase, and Science Direct databases were searched for studies that investigated associations of the FokI (rs2228570, rs10735810), and ApaI (rs7975253) polymorphisms of the VDR gene with DD...
October 31, 2016: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/27790329/vitamin-d-receptor-gene-matrix-metalloproteinase-3-polymorphisms-and-the-risk-of-intervertebral-disc-degeneration-susceptibility-meta-analysis
#12
REVIEW
Luming Nong, Yongjing Huang, Shujie Zhao, Nanwei Xu
Several studies have evaluated the association between vitamin D receptor, matrix metalloproteinase 3 (MMP-3) polymorphisms and the risk of intervertebral disc degeneration susceptibility. The findings were inconsistent. This meta-analysis aimed to systematically assess the association between vitamin D receptor, MMP-3 polymorphisms and the risk of intervertebral disc degeneration susceptibility. A search of various databases was done covering all papers published until December 31th, 2014. Eight, 4, 3 studies were finally included that addressed the risk of intervertebral disc degeneration susceptibility and vitamin D receptor FokI (rs2228570), ApaI (rs7975232), and MMP-3 (rs731236) polymorphisms, respectively...
October 2016: Asian Spine Journal
https://www.readbyqxmd.com/read/27786189/crispr-rna-guided-foki-nucleases-repair-a-pah-variant-in-a-phenylketonuria-model
#13
Yi Pan, Nan Shen, Sabine Jung-Klawitter, Christian Betzen, Georg F Hoffmann, Jörg D Hoheisel, Nenad Blau
The CRISPR/Cas9 system is a recently developed genome editing technique. In this study, we used a modified CRISPR system, which employs the fusion of inactive Cas9 (dCas9) and the FokI endonuclease (FokI-dCas9) to correct the most common variant (allele frequency 21.4%) in the phenylalanine hydroxylase (PAH) gene - c.1222C>T (p.Arg408Trp) - as an approach toward curing phenylketonuria (PKU). PKU is the most common inherited diseases in amino acid metabolism. It leads to severe neurological and neuropsychological symptoms if untreated or late diagnosed...
October 27, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27778467/association-between-vitamin-d-receptor-gene-polymorphism-and-ankylosing-spondylitis-in-han-chinese
#14
Pingping Zhang, Qiuxia Li, Jun Qi, Qing Lv, Xuqi Zheng, Xinyu Wu, Jieruo Gu
OBJECTIVES: To investigate whether vitamin D receptor (VDR) gene polymorphisms confer susceptibility to aankylosing spondylitis (AS) and study its polymorphisms in Han Chinese. METHODS: We screened single nucleotide polymorphisms (SNPs) in the VDR region through genome-wide genotyping chips in AS cases and healthy controls, then used the exome sequencing result to analyze all the potential AS-associated SNPs in the VDR gene. RESULTS: Thirty-two SNPs were found in the VDR gene in the genome-wide genotyping chips and the logistic regression result showed no significant difference between AS cases and controls...
October 25, 2016: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/27772736/vitamin-d-receptor-gene-polymorphisms-and-cognitive-decline-in-parkinson-s-disease
#15
Nicole M Gatto, Kimberly C Paul, Janet S Sinsheimer, Jeff M Bronstein, Yvette Bordelon, Rebecca Rausch, Beate Ritz
We and others have suggested that vitamin D receptor gene (VDR) polymorphisms influence susceptibility for Parkinson's disease (PD), Alzheimer's disease (AD), mild cognitive impairment (MCI) or overall cognitive functioning. Here we examine VDR polymorphisms and cognitive decline in patients with PD. Non-Hispanic Caucasian PD patients (n=190) in the Parkinson Environment Gene (PEG) study were successfully genotyped for seven VDR polymorphisms. Cognitive function was assessed with the Mini-Mental State Exam (MMSE) at baseline and at a maximum of three follow-up exams...
November 15, 2016: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/27771345/the-association-between-vitamin-d-receptor-polymorphisms-and-serum-25-hydroxyvitamin-d-levels-with-ulcerative-colitis-in-chinese-han-population
#16
Shu-Zi Zheng, Da-Guan Zhang, Hao Wu, Li-Jia Jiang, Jie Jin, Xiu-Qing Lin, Ran Ding, Yi Jiang
There is now growing evidence suggesting that Vitamin D is playing a critical role in modulating the innate and adaptive immune responses. Several polymorphisms have been identified in the vitamin D receptor (VDR) gene but their association with ulcerative colitis (UC) susceptibility remained controversy. In the current study, we examined the association between VDR polymorphisms and serum level of 25-hydroxyvitamin D [25(OH)D] with UC in Chinese Han population. Polymorphisms of FokI (rs2228570)/BsmI (rs1544410)/ApaI (rs7975232)/TaqI (rs731236) in the VDR gene were assessed in a case-control study comprising 404 UC patients and 612 controls...
October 19, 2016: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/27709568/all-in-one-crispr-cas9-foki-dcas9-vector-mediated-multiplex-genome-engineering-in-cultured-cells
#17
Tetsushi Sakuma, Takuya Sakamoto, Takashi Yamamoto
CRISPR-Cas9 enables highly convenient multiplex genome engineering in cultured cells, because it utilizes generic Cas9 nuclease and an easily customizable single-guide RNA (sgRNA) for site-specific DNA double-strand break induction. We previously established a multiplex CRISPR-Cas9 assembly system for constructing an all-in-one vector simultaneously expressing multiple sgRNAs and Cas9 nuclease or other Cas9 variants including FokI-dCas9, which supersedes the wild-type Cas9 with regard to high specificity. In this chapter, we describe a streamlined protocol to design and construct multiplex CRISPR-Cas9 or FokI-dCas9 vectors, to introduce them into cultured cells by lipofection or electroporation, to enrich the genomically edited cells with a transient puromycin selection, to validate the mutation efficiency by Surveyor nuclease assay, and to perform off-target analyses...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27695504/vitamin-d-receptor-gene-foki-polymorphisms-and-tuberculosis-susceptibility-a-meta-analysis
#18
Yan Cao, Xinjing Wang, Zhihong Cao, Xiaoxing Cheng
INTRODUCTION: The association between FokI polymorphism of vitamin D receptor (VDR) and tuberculosis (TB) susceptibility has been investigated previously; however, the results were inconsistent and conflicting. In the present study, a meta-analysis was performed to assess the relationship between VDR FokI gene polymorphism and the risk of TB. MATERIAL AND METHODS: Databases including PubMed and Embase were searched for genetic association studies of FokI polymorphism of vitamin D receptor (VDR) and TB...
October 1, 2016: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/27688524/taqi-foki-and-apai-polymorphisms-in-the-vitamin-d-receptor-in-beh%C3%A3-et-s-disease-in-turkish-population
#19
Gaye Erten, Muhammed Kalkan, Sema Bilgiç Gazioğlu, Nilgun Akdeniz, Elif Ozkok, Burcak Vural
Objectives. In our study we aimed to determine VDR gene polymorphisms in patients with Behçet's disease (BD) and neuro-Behçet's disease (NBD) in Turkish population. Methods. PBL obtained from 37 patients with BD, 21 patients with NB, and 30 healthy controls were investigated. Genomic DNA was extracted from whole blood using the QIAamp Blood Kit. VDR ApaI (rs7975232), VDR FokI (rs2228570), and VDR TaqI (rs731236) genotyping was performed by real-time polymerase chain reaction with SimpleProbe melting-curve analysis...
2016: Disease Markers
https://www.readbyqxmd.com/read/27686292/serum-vitamin-d-levels-and-gene-polymorphisms-fok1-and-apa1-in-children-with-type-i-diabetes-and-healthy-controls
#20
Maimoona Nasreen, Khalid Pervaiz Lone, Saba Khaliq, Shagufta Khaliq
OBJECTIVE: To compare the pattern of Vitamin D receptor (VDR) polymorphisms (Apa I and Fok I) in Type I Diabetes mellitus (T1DM) as cases vs healthy population as control and to investigate the association of VDR polymorphism with vitamin D levels in cases and controls. METHODS: The hypothesis of the study was "VDR gene polymorphisms (Fok 1 and Apa 1) and vitamin D levels are associated with the T1DM". The case-control study was carried out on 44 cases and 44 controls...
October 2016: JPMA. the Journal of the Pakistan Medical Association
keyword
keyword
103910
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"