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https://www.readbyqxmd.com/read/28381086/automated-quantitative-enzyme-biosensing-in-24-well-microplates
#1
Somjai Teanphonkrang, Albert Schulte
We report a novel system for glucose estimation in model and real samples, utilizing enzyme-modified pencil leads (PL) as effective electrochemical biosensors for robotic substrate quantification in 24-well microplates. Electrochemically formed carboxyl groups on the surface of the graphite were cross-linked to amino groups in the enzyme so as to attach glucose oxidase to the PL surface. Automated amperometric sensing of glucose solutions in microtiter-plate wells used computer-controlled stepper motors to move the biosensor/counter/reference electrode assemblies sequentially between the samples...
April 6, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28376986/transition-hand-off-from-inpatient-to-outpatient-treatment-of-acute-pyelonephritis-in-an-elderly-male
#2
Patricia L Orlando, Laura Shane-McWhorter
Pyelonephritis is the progression of a urinary tract infection (UTI) to the kidney. In younger patients the infection may not be as severe and may even be treated with oral antibiotics. However, in elderly males pyelonephritis can be more complex and may require hospitalization and treatment with intravenous antibiotics. In the United States UTIs are responsible for frequent visits to emergency departments by elderly individuals. Current literature suggests that pyelonephritis in elderly males is a serious infection that may result in significant morbidity and mortality...
April 1, 2017: Consultant Pharmacist: the Journal of the American Society of Consultant Pharmacists
https://www.readbyqxmd.com/read/28376849/effect-of-an-interactive-cardiopulmonary-resuscitation-assist-device-with-an-automated-external-defibrillator-synchronised-with-a-ventilator-on-the-cpr-performance-of-emergency-medical-service-staff-a-randomised-simulation-study
#3
Rainer Nitzschke, Christoph Doehn, Jan F Kersten, Julian Blanz, Tobias J Kalwa, Norman A Scotti, Jens C Kubitz
BACKGROUND: The present study evaluates whether the quality of advanced cardiac life support (ALS) is improved with an interactive prototype assist device. This device consists of an automated external defibrillator linked to a ventilator and provides synchronised visual and acoustic instructions for guidance through the ALS algorithm and assistance for face-mask ventilations. METHODS: We compared the cardiopulmonary resuscitation (CPR) quality of emergency medical system (EMS) staff members using the study device or standard equipment in a mannequin simulation study with a prospective, controlled, randomised cross-over study design...
April 4, 2017: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
https://www.readbyqxmd.com/read/28340542/expectations-and-attitudes-of-individuals-with-type-1-diabetes-after-using-a-hybrid-closed-loop-system
#4
Esti Iturralde, Molly L Tanenbaum, Sarah J Hanes, Sakinah C Suttiratana, Jodie M Ambrosino, Trang T Ly, David M Maahs, Diana Naranjo, Natalie Walders-Abramson, Stuart A Weinzimer, Bruce A Buckingham, Korey K Hood
Purpose The first hybrid closed loop (HCL) system, which automates insulin delivery but requires user inputs, was approved for treatment of type 1 diabetes (T1D) by the US Food and Drug Administration in September 2016. The purpose of this study was to explore the benefits, expectations, and attitudes of individuals with T1D following a clinical trial of an HCL system. Methods Thirty-two individuals with T1D (17 adults, 15 adolescents) participated in focus groups after 4 to 5 days of system use. Content analysis generated themes regarding perceived benefits, hassles, and limitations...
April 2017: Diabetes Educator
https://www.readbyqxmd.com/read/28334591/crossing-the-communication-chasm-challenges-and-opportunities-in-transitions-of-care-from-the-hospital-to-the-primary-care-clinic
#5
Nicholas A Rattray, Jason J Sico, LeeAnn M Cox, Alissa L Russ, Marianne S Matthias, Richard M Frankel
BACKGROUND: Transitions of care from specialty and acute settings to primary care abound. Compared to the continuity in end-of-shift handoffs, care transitions involve provider communication between practices and facilities with their own cultures and bureaucracies. Using the transition from acute care to outpatient primary care for stroke/transient ischemic attack (TIA) patients as a case study, this qualitative research explored communication practices and institutional arrangements among clinical providers responsible for longitudinal management of hypertension...
March 2017: Joint Commission Journal on Quality and Patient Safety
https://www.readbyqxmd.com/read/28328729/the-superiority-of-the-two-thumb-over-the-two-finger-technique-for-single-rescuer-infant-cardiopulmonary-resuscitation
#6
Sang Yoon Lee, Jun Young Hong, Je Hyeok Oh, Sun-Han Son
OBJECTIVES: The two-finger technique (TFT) using the index-middle fingers of the right hand (TFT-R23) was recently confirmed to produce deeper chest compression depth (CCD) compared with the TFT using any other fingers. This study was carried out to confirm whether the TFT-R23 would be as effective as the two-thumb technique (TTT). In addition, individual finger strengths were measured to identify the reasons why the TTT and TFT-R23 produced deeper CCD than any other methods. METHODS: This prospective randomized cross-over trial compared TTT with TFT-R23...
March 21, 2017: European Journal of Emergency Medicine: Official Journal of the European Society for Emergency Medicine
https://www.readbyqxmd.com/read/28325473/foxh1-occupies-cis-regulatory-modules-prior-to-dynamic-transcription-factor-interactions-controlling-the-mesendoderm-gene-program
#7
Rebekah M Charney, Elmira Forouzmand, Jin Sun Cho, Jessica Cheung, Kitt D Paraiso, Yuuri Yasuoka, Shuji Takahashi, Masanori Taira, Ira L Blitz, Xiaohui Xie, Ken W Y Cho
The interplay between transcription factors and chromatin dictates gene regulatory network activity. Germ layer specification is tightly coupled with zygotic gene activation and, in most metazoans, is dependent upon maternal factors. We explore the dynamic genome-wide interactions of Foxh1, a maternal transcription factor that mediates Nodal/TGF-β signaling, with cis-regulatory modules (CRMs) during mesendodermal specification. Foxh1 marks CRMs during cleavage stages and recruits the co-repressor Tle/Groucho in the early blastula...
March 27, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28304316/perspectives-and-attitudes-of-pediatricians-concerning-post-discharge-care-practice-of-premature-infants
#8
A Gad, E Parkinson, N Khawar, A Elmeki, P Narula, D Hoang
OBJECTIVE: Survival rates of premature infants are at a historical high and increasingly more pediatricians are caring for former premature infants. The goal of this study was to describe the perspectives and attitudes of pediatricians, as well as, the challenges of rendering post-neonatal intensive care unit (NICU) discharge care for premature infants. METHODS: An anonymous 22-question web-based survey was emailed to pediatricians who are current members of the American Academy of Pediatrics (AAP) and practicing in Kings County, New York...
March 16, 2017: Journal of Neonatal-perinatal Medicine
https://www.readbyqxmd.com/read/28275613/development-of-a-new-interfacility-extracorporeal-membrane-oxygenation-transport-program-for-pediatric-lung-transplantation-evaluation
#9
REVIEW
W Joshua Frazier, Edward G Shepherd, Samantha W Gee
Pediatric lung transplantation is a life-saving intervention for children with irreversible end-stage lung disease. Access to transplant can be limited by geographic isolation from a center or the presence of comorbidities affecting transplant eligibility. Extracorporeal membrane oxygenation (ECMO)-supported patients are an uncommon but historically high-risk cohort of patients considered for lung transplant. We report the development of a service at our center to provide transport services to our hospital for patients unable to wean from ECMO support at their local institution for the purpose of evaluation for lung transplantation by our program...
February 2017: Annals of Translational Medicine
https://www.readbyqxmd.com/read/28241908/implementation-and-evaluation-of-the-safety-net-specialty-care-program-in-the-denver-metropolitan-area
#10
Meredith P Fort, Lynnette M Namba, Sarah Dutcher, Tracy Copeland, Neysa Bermingham, Chris Fellenz, Deborah Lantz, John J Reusch, Elizabeth A Bayliss
OBJECTIVES: In response to limited access to specialty care in safety-net settings, an integrated delivery system and three safety-net organizations in the Denver, CO, metropolitan area launched a unique program in 2013. The program offers safety-net providers the option to electronically consult with specialists. Uninsured patients may be seen by specialists in office visits for a defined set of services. This article describes the program, identifies aspects that have worked well and areas that need improvement, and offers lessons learned...
2017: Permanente Journal
https://www.readbyqxmd.com/read/28174647/the-art-of-vaginal-breech-birth-at-term-on-all-fours
#11
Hajo I J Wildschut, Hinke van Belzen-Slappendel, Suze Jans
Despite a shift in clinical practice favouring cesarean section for breech presentation, adequate skills are still needed for a safe vaginal breech birth. This case report illustrates the physiological mechanism of vaginal breech birth. The accompanying pictures are a testimony to the "hands-off" approach and could be used for educational purposes.
February 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/28167175/effect-of-melt-method-on-thoracolumbar-connective-tissue-the-full-study
#12
Faria Sanjana, Hans Chaudhry, Thomas Findley
Altered connective tissue structure has been identified in adults with chronic low back pain (LBP). A self-care treatment for managing LBP is the MELT method. The MELT method is a hands-off, self-treatment that is said to alleviate chronic pain, release tension and restore mobility, utilizing specialized soft treatments balls, soft body roller and techniques mimicking manual therapy. The objective of this study was to determine whether thickness of thoracolumbar connective tissue and biomechanical and viscoelastic properties of myofascial tissue in the low back region change in subjects with chronic LBP as a result of MELT...
January 2017: Journal of Bodywork and Movement Therapies
https://www.readbyqxmd.com/read/28152762/ro-ils-radiation-oncology-incident-learning-system-data-trends-2014-2015
#13
Eric C Ford, Nadine L Eads, Ksenija Kapetanovic, Cindy Tomlinson
59 Background: Incident learning is one of the most effective ways to improve quality care. To facilitate patient safety improvement at a national level, American Society for Radiation Oncology (ASTRO) and American Association of Physicists in Medicine (AAPM) launched RO-ILS: Radiation Oncology Incident Learning System in June 2014. RO-ILS mission is to facilitate safer and higher quality care through a shared learning environment that is secure and non-punitive. METHODS: To ensure the security and protection of data, ASTRO contracted with Clarity PSO, a federally-certified patient safety organization that operates under the auspices of the Patient Safety and Quality Improvement Act of 2005...
March 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28152685/optimizing-discharge-teaching-process-to-increase-the-efficiency-and-effectiveness-of-the-follow-up-call
#14
Gina Aranzamendez, Beverly A Hayes, Kavita Pathak, Duke Rohe, Valerie Haywood
226 Background: Patients undergoing complex oncologic operations are at high risk for perioperative complications associated with adverse effects on quality of life, costs and overall outcomes. Complications within 30 days of discharge are most commonly identified during first 48-72 hours post-discharge. Telephone follow-up can provide a safety net and is part of a larger discharge teaching process. Initial review of the current follow up processes underscored many variations. An interdisciplinary team was tasked to optimize discharge processes across the continuum of care which leads to a more efficient follow-up call encounter...
March 2016: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
https://www.readbyqxmd.com/read/28132428/us-physician-and-midwife-adherence-to-active-management-of-the-third-stage-of-labor-international-recommendations
#15
Mavis N Schorn, Mary S Dietrich, Beth Donaghey, Ann F Minnick
INTRODUCTION: The prevalence of postpartum hemorrhage has increased in the United States despite the international promotion of active management of the third stage of labor. Adherence to the international recommendations in the United States is unclear. It is also not known how the components of active management are related to other practices that may be used during the third stage of labor. The purpose of this study was to determine routine practice patterns for managing the third stage of labor in the United States...
January 2017: Journal of Midwifery & Women's Health
https://www.readbyqxmd.com/read/28128492/beyond-attachment-roles-of-dc-sign-in-dengue-virus-infection
#16
Ping Liu, Marc Ridilla, Pratik Patel, Laurie Betts, Emily Gallichotte, Lidea Shahidi, Nancy L Thompson, Ken Jacobson
Dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN), a C-type lectin expressed on the plasma membrane by human immature dendritic cells, is a receptor for numerous viruses including Ebola, SARS and dengue. A controversial question has been whether DC-SIGN functions as a complete receptor for both binding and internalization of dengue virus (DENV) or whether it is solely a cell surface attachment factor, requiring either hand-off to another receptor or a co-receptor for internalization...
January 27, 2017: Traffic
https://www.readbyqxmd.com/read/28125898/mechanisms-and-factors-associated-with-tackle-related-injuries-in-south-african-youth-rugby-union-players
#17
Nicholas Burger, Mike Ian Lambert, Wayne Viljoen, James Craig Brown, Clint Readhead, Steve den Hollander, Sharief Hendricks
BACKGROUND: The majority of injuries in rugby union occur during tackle events. The mechanisms and causes of these injuries are well established in senior rugby union. PURPOSE: To use information from an injury database and assess video footage of tackle-related injuries in youth rugby union matches to identify environmental factors and mechanisms that are potentially confounding to these injuries. STUDY DESIGN: Descriptive epidemiological study...
February 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28106651/evaluating-the-impact-of-intraoperative-surgical-team-handoffs-on-patient-outcomes
#18
Lauren E Giugale, Sarah Sears, Erin S Lavelle, Charelle M Carter-Brooks, Michael Bonidie, Jonathan P Shepherd
OBJECTIVE: The aim of the study was to assess the impact of intraoperative personnel handoffs on clinical outcomes in patients undergoing minimally invasive sacrocolpopexy (SCP). METHODS: We retrospectively reviewed SCPs performed at an academic center between 2009 and 2014. We analyzed the number of staff handoffs, defined as any instance a scrub technician (tech) or circulating nurse handed off responsibility for a break or shift change. Outcomes included operative (OR) time and composite variables for major complications (conversion to an open procedure, bladder injury, bowel injury, blood transfusion, infection, ileus, bowel obstruction, readmission, or mesh complication) and prolapse recurrence (prolapse at or beyond the hymen or retreatment)...
January 18, 2017: Female Pelvic Medicine & Reconstructive Surgery
https://www.readbyqxmd.com/read/28097858/-the-current-status-of-patient-safety-in-argentina-cross-sectional-study
#19
Lucrecia Arpí, Néstor D Panattieri, Cristina Godio, Verónica Sabio Paz, Nora Dackiewicz
BACKGROUND: Patient safety is a priority for healthcare organizations. For the PRONAP´s 2013 final exam, the Quality & Patient Safety Subcommittee and the PRONAP managers designed a survey to be answered by pediatrician students nationwide. It was destined to evaluate attitudes, practices and safety conditions in which they worked. AIM: To assess the current state of practices in patient safety. MATERIAL AND METHODS: Setting and sample: PRONAP students (7,438 pediatrician nationwide) who answered 2013 final exam...
February 1, 2017: Archivos Argentinos de Pediatría
https://www.readbyqxmd.com/read/28078762/competence-in-the-use-of-supraglottic-airways-by-australian-surf-lifesavers-for-cardiac-arrest-ventilation-in-a-manikin
#20
Lachlan Holbery-Morgan, Cara Angel, Michelle Murphy, James Carew, Finn Douglas, Robert Murphy, Natalie Hood, Andrew Rechtman, Christopher Scarff, Nicholas Simpson, Andrew Stewardson, Daniel Steinfort, Sam Radford, Ned Douglas, Douglas Johnson
OBJECTIVES: Lifesavers in Australia are taught to use pocket mask (PM) rescue breathing and bag valve mask (BVM) ventilation, despite evidence that first responders might struggle with these devices. Novices have successfully used the Laryngeal Mask Airway (LMA) Supreme and iGel devices previously, but there has been no previous comparison of the ability to train lifesavers to use the supraglottic airways compared to standard techniques for cardiac arrest ventilation. METHODS: The study is a prospective educational intervention whereby 113 lifesavers were trained to use the LMA and iGel supraglottic airways...
February 2017: Emergency Medicine Australasia: EMA
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