Christiane Brugger, Jacob Schwartz, Scott Novick, Song Tong, Joel Hoskins, Nadim Majdalani, Rebecca Kim, Martin Filipovski, Sue Wickner, Susan Gottesman, Patrick Griffin, Alexandra M Deaconescu
In enterobacteria such as Escherichia coli, the general stress response is mediated by σs , the stationary phase dissociable promoter specificity subunit of RNA polymerase. σs is degraded by ClpXP during active growth in a process dependent on the RssB adaptor, which is thought to be stimulated by phosphorylation of a conserved aspartate in its N-terminal receiver domain. Here we present the crystal structure of full-length RssB bound to a beryllofluoride phosphomimic. Compared to the structure of RssB bound to the IraD anti-adaptor, our new RssB structure with bound beryllofluoride reveals conformational differences and coil-to-helix transitions in the C-terminal region of the RssB receiver domain and in the inter-domain segmented helical linker...
November 8, 2023: Journal of Biological Chemistry