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https://www.readbyqxmd.com/read/28544311/impact-of-body-mass-index-on-robotic-radical-cystectomy-with-intra-corporeal-urinary-diversion
#1
Nariman Ahmadi, Thomas G Clifford, Gus Miranda, Jie Cai, Monish Aron, Mihir M Desai, Inderbir S Gill
OBJECTIVES: To determine the impact of body mass index (BMI) on peri-operative and oncological outcomes following robotic radical cystectomy (RRC) with intra-corporeal urinary diversion (ICUD). SUBJECTS AND METHODS: A total of 216 patients undergoing RRC, extended lymphadenectomy and ICUD (07/2010-12/2015) were categorized into four BMI groups according to the 2004 World Health Organization (WHO) obesity classification: <25 kg/m(2) (normal), 25-29.9 kg/m(2) (pre-obese), 30-34...
May 22, 2017: BJU International
https://www.readbyqxmd.com/read/28520714/alteration-of-stream-temperature-by-natural-and-artificial-beaver-dams
#2
Nicholas Weber, Nicolaas Bouwes, Michael M Pollock, Carol Volk, Joseph M Wheaton, Gus Wathen, Jacob Wirtz, Chris E Jordan
Beaver are an integral component of hydrologic, geomorphic, and biotic processes within North American stream systems, and their propensity to build dams alters stream and riparian structure and function to the benefit of many aquatic and terrestrial species. Recognizing this, beaver relocation efforts and/or application of structures designed to mimic the function of beaver dams are increasingly being utilized as effective and cost-efficient stream and riparian restoration approaches. Despite these verities, the notion that beaver dams negatively impact stream habitat remains common, specifically the assumption that beaver dams increase stream temperatures during summer to the detriment of sensitive biota such as salmonids...
2017: PloS One
https://www.readbyqxmd.com/read/28505352/the-nodulin-26-like-intrinsic-membrane-protein-osnip3-2-is-involved-in-arsenite-uptake-by-lateral-roots-in-rice
#3
Yi Chen, Sheng-Kai Sun, Zhong Tang, Guidong Liu, Katie L Moore, Frans J M Maathuis, Antony J Miller, Steve P McGrath, Fang-Jie Zhao
Previous studies have shown that the Nodulin 26-like intrinsic membrane protein (NIP) Lsi1 (OsNIP2;1) is involved in arsenite [As(III)] uptake in rice (Oryza sativa). However, the role of other rice NIPs in As(III) accumulation in planta remains unknown. In the present study, we investigated the role OsNIP3;2 in As(III) uptake in rice. When expressed in Xenopus laevis oocytes, OsNIP3;2 showed a high transport activity for As(III). Quantitative real-time RT-PCR showed that the expression of OsNIP3;2 was suppressed by 5 µM As(III), but enhanced by 20 and 100 µM As(III)...
May 15, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28462487/psychopathology-and-friendship-in-children-and-adolescents-disentangling-the-role-of-co-occurring-symptom-domains-with-serial-mediation-models
#4
Arthur Gus Manfro, Pedro M Pan, Ary Gadelha, Marcelo Fleck, Maria C do Rosário, Hugo Cogo-Moreira, Rodrigo Affonseca-Bressan, Jair Mari, Euripedes C Miguel, Luis A Rohde, Giovanni A Salum
The consolidation of social friendship groups is a vital part of human development. The objective of this study is to understand the direct and indirect influences of three major symptomatic domains-emotional, hyperkinetic, and conduct-on friendship. Specifically, we aim to study if the associations of one domain with friendship may be mediated by co-occurring symptoms from another domain. A total of 2512 subjects aged 6-14 years participated in this study. Friendship was evaluated by the Development and Well-Being Assessment's friendship section...
May 2, 2017: European Child & Adolescent Psychiatry
https://www.readbyqxmd.com/read/28430019/characterization-of-rph24-a-gene-conferring-adult-plant-resistance-to-puccinia-hordei-in-barley
#5
Laura A Ziems, Lee T Hickey, Gregory J Platz, Jerome D Franckowiak, Peter M Dracatos, Davinder Singh, Robert F Park
We identified Rph24 as a locus in barley (Hordeum vulgare L.) controlling adult plant resistance (APR) to leaf rust, caused by Puccinia hordei. The locus was previously reported as a quantitative trait locus in barley line ND24260-1 and named qRphND. We crossed ND24260-1 to the leaf-rust-susceptible standard Gus and determined inheritance patterns in the progeny. For the comparative marker frequency analysis (MFA), resistant and susceptible tails of the F2 were genotyped with Diversity Arrays Technology genotyping-by-sequencing (DArT-Seq) markers...
April 21, 2017: Phytopathology
https://www.readbyqxmd.com/read/28392226/the-relationship-of-plasma-creatinine-as-egfr-and-high-sensitivity-cardiac-troponin-and-nt-probnp-concentrations-in-a-hospital-and-community-outpatient-population
#6
Julia M Potter, Aaron J Simpson, Jennifer Kerrigan, Emma Southcott, Marie M Salib, Gus Koerbin, Peter E Hickman
OBJECTIVES: While persons with overt renal failure have a well-described rise in troponin and NT-proBNP, it is less well described what the relationship is between cardiac markers and persons with impaired renal function, not requiring dialysis. DESIGN & METHODS: We have collected ALL samples referred to our pathology practice over a 24h period and measured hs-cTnI, hs-cTnT, NT-proBNP, calculated the eGFR, and related our measurements to clinical outcomes. RESULTS: For both men and women, for all of hs-cTnI, hs-cTnT and NT-proBNP, there was a graded response, as renal function worsened, the concentration of the cardiac marker increased...
April 6, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28287543/modified-drop-tower-impact-tests-for-american-football-helmets
#7
G Alston Rush, R Prabhu, Gus A Rush, Lakiesha N Williams, M F Horstemeyer
A modified National Operating Committee on Standards for Athletic Equipment (NOCSAE) test method for American football helmet drop impact test standards is presented that would provide better assessment of a helmet's on-field impact performance by including a faceguard on the helmet. In this study, a merger of faceguard and helmet test standards is proposed. The need for a more robust systematic approach to football helmet testing procedures is emphasized by comparing representative results of the Head Injury Criterion (HIC), Severity Index (SI), and peak acceleration values for different helmets at different helmet locations under modified NOCSAE standard drop tower tests...
February 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28265995/analyzing-cytokinin-responses-during-plant-nematode-interactions
#8
Florian M W Grundler, Shahid Siddique
Cyst nematodes are obligate biotrophs that induce the formation of a hypertrophied and hypermetabolic syncytial-feeding site in roots of the host plants. Cytokinin signaling is activated at the site of infection and contributes significantly to the formation of syncytium. Here, we describe a protocol for visualizing cytokinin signaling activation in Arabidopsis upon infection with cyst nematode Heterodera schachtii using promoter reporter lines, TCSn:GFP and ARR5:GUS.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28263716/statistical-considerations-for-determining-high-sensitivity-cardiac-troponin-reference-intervals
#9
Peter E Hickman, Gus Koerbin, Julia M Potter, Walter P Abhayaratna
The troponin 99th percentile is used as the laboratory decision point in the diagnosis of acute myocardial infarction. A recent publication has shown that the statistical treatment for outlier removal may dramatically change the calculated troponin 99th percentile. We have used our large database from the previously reported Canberra Heart Study to independently assess the effect of various methods for removing outliers on the calculated 99th percentile. We have performed the same exercise using the troponin 97...
March 1, 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28208118/validation-of-a-novel-monitoring-system-to-measure-contrast-volume-use-during-invasive-angiography
#10
Anand Prasad, Irma Scholler, Daniel Levin, Gus Banda, Christopher M Mullin, Steven R Bailey
BACKGROUND: Multiple studies have demonstrated the relationship between contrast volume (CV) and the risk of acute kidney injury (AKI). Quantification of total CV is often estimated and therefore may be inaccurate. We describe validation of a novel contrast monitoring system (CMS) (Osprey Medical), which is designed to detect and display injection by injection and total CV use on a monitor system in real time. METHODS: Thirty patients undergoing a coronary or peripheral angiogram were included...
February 15, 2017: Journal of Invasive Cardiology
https://www.readbyqxmd.com/read/28112257/ethylene-response-pathway-modulates-attractiveness-of-plant-roots-to-soybean-cyst-nematode-heterodera-glycines
#11
Yanfeng Hu, Jia You, Chunjie Li, Valerie M Williamson, Congli Wang
Plant parasitic nematodes respond to root exudates to locate their host roots. In our studies second stage juveniles of Heterodera glycines, the soybean cyst nematode (SCN), quickly migrated to soybean roots in Pluronic F-127 gel. Roots of soybean and non-host Arabidopsis treated with the ethylene (ET)-synthesis inhibitor aminoethoxyvinylglycine (AVG) were more attractive to SCN than untreated roots, and significantly more nematodes penetrated into roots. Moreover, Arabidopsis ET insensitive mutants (ein2, ein2-1, ein2-5, ein3-1, ein5-1, and ein6) were more attractive than wild-type plants...
January 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28105851/in-situ-hybridization-and-promoter-analysis-reveal-that-cotton-leaf-curl-multan-betasatellite-localizes-in-the-phloem
#12
O Eini, M S Rasheed, J W Randles
Begomoviruses (the family Geminiviridae) have either a monopartite or a bipartite (DNA A and DNA B) single-stranded DNA genome. DNA B contains the open reading frame for movement protein and enables some bipartite begomoviruses to invade non-phloem tissues, whereas monopartite begomoviruses are limited to the phloem. Betasatellite DNA replicates in association with some monopartite begomoviruses to produce severe symptoms and enhance replication of helper virus in some hosts. The cotton leaf curl Multan betasatellite (CLCuMuB) has been shown to substitute for the DNA B of a bipartite begomovirus by permitting systemic infection, but the tissue tropism and the role of betasatellites in releasing monopartite begomoviruses from the phloem to adjacent tissue has not been described...
2017: Acta Virologica
https://www.readbyqxmd.com/read/28079296/elasticity-in-macrophage-synthesized-biocrystals
#13
Elizabeth M Horstman, Rahul K Keswani, Benjamin A Frey, Phillip M Rzeczycki, Vernon LaLone, Jeffery A Bertke, Paul J A Kenis, Gus R Rosania
Supramolecular crystalline assembly constitutes a rational approach to bioengineer intracellular structures. Here, biocrystals of clofazimine (CFZ) that form in vivo within macrophages were measured to have marked curvature. Isolated crystals, however, showed reduced curvature suggesting that intracellular forces bend these drug crystals. Consistent with the ability of biocrystals to elastically deform, the inherent crystal structure of the principal molecular component of the biocrystals-the hydrochloride salt of CFZ (CFZ-HCl)-has a corrugated packing along the (001) face and weak dispersive bonding in multiple directions...
January 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28030617/sublethal-effects-of-imidacloprid-on-honey-bee-colony-growth-and-activity-at-three-sites-in-the-u-s
#14
William G Meikle, John J Adamczyk, Milagra Weiss, Ales Gregorc, Don R Johnson, Scott D Stewart, Jon Zawislak, Mark J Carroll, Gus M Lorenz
Imidacloprid is a neonicotinoid pesticide heavily used by the agricultural industry and shown to have negative impacts on honey bees above certain concentrations. We evaluated the effects of different imidacloprid concentrations in sugar syrup using cage and field studies, and across different environments. Honey bee colonies fed sublethal concentrations of imidicloprid (0, 5, 20 and 100 ppb) over 6 weeks in field trials at a desert site (Arizona), a site near intensive agriculture (Arkansas) and a site with little nearby agriculture but abundant natural forage (Mississippi) were monitored with respect to colony metrics, such as adult bee and brood population sizes, as well as pesticide residues...
2016: PloS One
https://www.readbyqxmd.com/read/28009322/predicting-of-risks-of-groundwater-and-surface-water-pollution-with-different-classes-of-herbicides-in-soil-in-eastern-europe-climate-conditions
#15
M Korshun, O Dema, O Kucherenko, O Korshun, S Garkavyi, I Pelio, A Antonenko, N Velikaia
Application of pesticides in modern agriculture is a powerful permanent risk factor for public health and the natural environment. The aim of the study was a comparative hygienic assessment of the danger of contamination of ground and surface water sources with most widely used herbicides of different chemical classes (sulfonylureas, imidazolinones, pirimidinilkarboksilovye compounds semicarbazones). Field hygienic experiments for studying of the residues dynamics of studied herbicides concentration in agrocenosis objects were made by us in different types of soils: chernozem, sod-podzolic, podzolized forest...
November 2016: Georgian Medical News
https://www.readbyqxmd.com/read/28007029/arabidopsis-prc1-core-component-atring1-regulates-stem-cell-determining-carpel-development-mainly-through-repression-of-class-i-knox-genes
#16
Donghong Chen, Anne M Molitor, Lin Xu, Wen-Hui Shen
BACKGROUND: Polycomb repressive complex 2 (PRC2)-catalyzed H3K27me3 marks are tightly associated with the WUS-AG negative feedback loop to terminate floral stem cell fate to promote carpel development, but the roles of Polycomb repressive complex 1 (PRC1) in this event remain largely uncharacterized. RESULTS: Here we show conspicuous variability in the morphology and number of carpels among individual flowers in the absence of the PRC1 core components AtRING1a and AtRING1b, which contrasts with the wild-type floral meristem consumed by uniform carpel production in Arabidopsis thaliana...
December 22, 2016: BMC Biology
https://www.readbyqxmd.com/read/27965209/effectiveness-of-chlorthalidone-plus-amiloride-for-the-prevention-of-hypertension-the-prever-prevention-randomized-clinical-trial
#17
Sandra Costa Fuchs, Carlos E Poli-de-Figueiredo, José A Figueiredo Neto, Luiz César N Scala, Paul K Whelton, Francisca Mosele, Renato Bandeira de Mello, José F Vilela-Martin, Leila B Moreira, Hilton Chaves, Marco Mota Gomes, Marcos R de Sousa, Ricardo Pereira E Silva, Iran Castro, Evandro José Cesarino, Paulo Cesar Jardim, João Guilherme Alves, André Avelino Steffens, Andréa Araujo Brandão, Fernanda M Consolim-Colombo, Paulo Ricardo de Alencastro, Abrahão Afiune Neto, Antônio C Nóbrega, Roberto Silva Franco, Dario C Sobral Filho, Alexandro Bordignon, Fernando Nobre, Rosane Schlatter, Miguel Gus, Felipe C Fuchs, Otávio Berwanger, Flávio D Fuchs
BACKGROUND: Prehypertension is associated with higher cardiovascular risk, target organ damage, and incidence of hypertension. The Prevention of Hypertension in Patients with PreHypertension (PREVER-Prevention) trial aimed to evaluate the efficacy and safety of a low-dose diuretic for the prevention of hypertension and end-organ damage. METHODS AND RESULTS: This randomized, parallel, double-blind, placebo-controlled trial was conducted in 21 Brazilian academic medical centers...
December 13, 2016: Journal of the American Heart Association
https://www.readbyqxmd.com/read/27918241/detection-of-oil-palm-root-penetration-by-agrobacterium-mediated-transformed-ganoderma-boninense-expressing-green-fluorescent-protein
#18
Nisha Govender, Mui-Yun Wong
A highly efficient and reproducible Agrobacterium-mediated transformation protocol for Ganoderma boninense was developed to facilitate observation of the early stage infection of basal stem rot (BSR). The method was proven amenable to different explants (basidiospore, protoplast, and mycelium) of G. boninense. The transformation efficiency was highest (62%) under a treatment combination of protoplast explant and Agrobacterium strain LBA4404, with successful expression of an hyg marker gene and gus-gfp fusion gene under the control of heterologous p416 glyceraldehyde 3-phosphate dehydrogenase promoter...
April 2017: Phytopathology
https://www.readbyqxmd.com/read/27889363/new-tools-for-the-hydromorphological-assessment-and-monitoring-of-european-streams
#19
M Rinaldi, B Belletti, M Bussettini, F Comiti, B Golfieri, B Lastoria, E Marchese, L Nardi, N Surian
Hydromorphological stream assessment has significantly expanded over the last years, but a need has emerged from recent reviews for more comprehensive, process-based methods that consider the character and dynamics of the river with greater accuracy. With this as a focus, a series of hydromorphological tools have been developed and/or further extended in Europe within the context of the REFORM (REstoring rivers FOR effective catchment Management) project. The aim of this paper is to present the set of REFORM hydromorphological assessment methods and, based on some examples of their application, to illustrate and discuss their synergic use, specific features, limitations and strengths...
November 23, 2016: Journal of Environmental Management
https://www.readbyqxmd.com/read/27872504/harmonising-reference-intervals-for-three-calculated-parameters-used-in-clinical-chemistry
#20
REVIEW
David Hughes, Gus Koerbin, Julia M Potter, Nicholas Glasgow, Nic West, Walter P Abhayaratna, Juleen Cavanaugh, David Armbruster, Peter E Hickman
For more than a decade there has been a global effort to harmonise all phases of the testing process, with particular emphasis on the most frequently utilised measurands. In addition, it is recognised that calculated parameters derived from these measurands should also be a target for harmonisation. Using data from the Aussie Normals study we report reference intervals for three calculated parameters: serum osmolality, serum anion gap and albumin-adjusted serum calcium. The Aussie Normals study was an a priori study that analysed samples from 1856 healthy volunteers...
August 2016: Clinical Biochemist. Reviews
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