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https://www.readbyqxmd.com/read/28893220/the-added-value-of-musculoskeletal-ultrasound-to-clinical-evaluation-in-the-treatment-decision-of-rheumatoid-arthritis-outpatients-physician-experience-matters
#1
C Sifuentes-Cantú, I Contreras-Yáñez, L Saldarriaga, A C Lozada, M Gutiérrez, V Pascual-Ramos
BACKGROUND: Musculoskeletal ultrasound improves the accuracy of detecting the level of disease activity (DA) in RA patients, although its impact on the final treatment decision in a real clinical setting is uncertain. The objectives were to define the percentage of clinical scenarios from an ongoing cohort of RA outpatients in which the German Ultrasound Score on 7 joints (GUS-7) impacted the treatment and to explore if the impact differed between a senior rheumatologist (SR) vs. a trainee (TR)...
September 11, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28878804/the-latex-protein-mlx56-from-mulberry-morus-multicaulis-protects-plants-against-insect-pests-and-pathogens
#2
Ying-Ping Gai, Ya-Nan Zhao, Huai-Ning Zhao, Chuan-Zhong Yuan, Shuo-Shuo Yuan, Shuo Li, Bing-Sen Zhu, Xian-Ling Ji
Biotic stresses are major constraints limiting the leaf quality and productivity of mulberry. MLX56 is a unique chitin-binding protein isolated from Shin-Ichinose (Morus alba) latex that displays toxicity against lepidopteran caterpillars. In this study, the full-length cDNA encoding MLX56 was isolated from Husang 32 (M. multicaulis) and designated HMLX56. Amino acid sequence analysis and protein modeling of three MLX56 proteins showed that they were highly conserved among Morus species. Tissue expression pattern analysis showed that the HMLX56 gene was strongly expressed in mulberry bark and leaves but only slightly expressed in fruits...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28878506/analysis-of-tandem-repeat-units-of-the-promoter-of-capsanthin-capsorubin-synthase-ccs-gene-in-pepper-fruit
#3
Shi-Lin Tian, Zheng Li, Li Li, S N M Shah, Zhen-Hui Gong
Capsanthin/capsorubin synthase (Ccs) gene is a key gene that regulates the synthesis of capsanthin and the development of red coloration in pepper fruits. There are three tandem repeat units in the promoter region of Ccs, but the potential effects of the number of repetitive units on the transcriptional regulation of Ccs has been unclear. In the present study, expression vectors carrying different numbers of repeat units of the Ccs promoter were constructed, and the transient expression of the β-glucuronidase (GUS) gene was used to detect differences in expression levels associated with the promoter fragments...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28842561/construction-and-in-vivo-assembly-of-a-catalytically-proficient-and-hyperthermostable-de-novo-enzyme
#4
Daniel W Watkins, Jonathan M X Jenkins, Katie J Grayson, Nicola Wood, Jack W Steventon, Kristian K Le Vay, Matthew I Goodwin, Anna S Mullen, Henry J Bailey, Matthew P Crump, Fraser MacMillan, Adrian J Mulholland, Gus Cameron, Richard B Sessions, Stephen Mann, J L Ross Anderson
Although catalytic mechanisms in natural enzymes are well understood, achieving the diverse palette of reaction chemistries in re-engineered native proteins has proved challenging. Wholesale modification of natural enzymes is potentially compromised by their intrinsic complexity, which often obscures the underlying principles governing biocatalytic efficiency. The maquette approach can circumvent this complexity by combining a robust de novo designed chassis with a design process that avoids atomistic mimicry of natural proteins...
August 25, 2017: Nature Communications
https://www.readbyqxmd.com/read/28755726/the-underappreciated-doctors-of-the-american-civil-rights-movement-part-i-theodore-roosevelt-mason-howard-md
#5
EDITORIAL
Richard D deShazo, Sara B Parker
During the fight to end segregation in the United States, most of the 25 or so black physicians who had not already left Mississippi took risks to become active in civil rights locally and nationally. One of the first was T.R.M. Howard, MD, whose life story is both an encouragement and warning for today's physicians. Howard, the protégé of a white Adventist physician, became active in civil rights during medical school. While serving as chief surgeon of the all-black hospital in Mississippi, he formed his own civil rights organization in 1951 and worked to solve the shootings of 2 of its members, George Lee and Gus Courts, and the murder of Emmett Till in 1955...
July 2017: American Journal of the Medical Sciences
https://www.readbyqxmd.com/read/28755354/transient-gus-gene-expression-in-date-palm-fruit-using-agroinjection-transformation-technique
#6
Mohei El-Din M Solliman, Hebaallah A Mohasseb, Abdullatif A Al-Khateeb, Jameel M Al-Khayri, Suliman A Al-Khateeb
Transient expression of foreign genes in plant tissue is a valuable tool for testing the efficacy of transformation methods. In this work, we present, for the first time, the utilization of agroinjection as an efficient transformation system for gene delivery in date palm fruit. The research utilized Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pRI201-AN-GUS carrying the beta-glucuronidase (GUS) gene, under the control of a CaMV 35S and kanamycin (NPTII) as an antibiotic gene under the control of a NOS promoter...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28746737/divergent-expression-of-cytokinin-biosynthesis-signaling-and-catabolism-genes-underlying-differences-in-feeding-sites-induced-by-cyst-and-root-knot-nematodes
#7
Carola D Dowd, Demosthenis Chronis, Zoran S Radakovic, Shahid Siddique, Thomas Schmülling, Tomáš Werner, Tatsuo Kakimoto, Florian M W Grundler, Melissa G Mitchum
Cyst and root-knot nematodes are obligate parasites of economic importance with a remarkable ability to reprogram root cells into unique metabolically active feeding sites. Previous studies have suggested a role for cytokinin in feeding site formation induced by these two types of nematodes, but the mechanistic details have not yet been described. Using Arabidopsis as a host plant species, we conducted a comparative analysis of cytokinin genes in response to the beet cyst nematode (BCN), Heterodera schachtii, and the root-knot nematode (RKN), Meloidogyne incognita...
July 26, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28611805/transcriptomic-analysis-in-strawberry-fruits-reveals-active-auxin-biosynthesis-and-signaling-in-the-ripe-receptacle
#8
Elizabeth Estrada-Johnson, Fabiana Csukasi, Carmen M Pizarro, José G Vallarino, Yulia Kiryakova, Amalia Vioque, Javier Brumos, Nieves Medina-Escobar, Miguel A Botella, José M Alonso, Alisdair R Fernie, José F Sánchez-Sevilla, Sonia Osorio, Victoriano Valpuesta
The role of auxin in ripening strawberry (Fragaria ×ananassa) fruits has been restricted to the early stages of development where the growth of the receptacle is dependent on the delivery of auxin from the achenes. At later stages, during enlargement of the receptacle, other hormones have been demonstrated to participate to different degrees, from the general involvement of gibberellins and abscisic acid to the more specific of ethylene. Here we report the involvement of auxin at the late stages of receptacle ripening...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28603536/molecular-transducers-from-roots-are-triggered-in-arabidopsis-leaves-by-root-knot-nematodes-for-successful-feeding-site-formation-a-conserved-post-embryogenic-de-novo-organogenesis-program
#9
Rocío Olmo, Javier Cabrera, Miguel A Moreno-Risueno, Hidehiro Fukaki, Carmen Fenoll, Carolina Escobar
Root-knot nematodes (RKNs; Meloidogyne spp.) induce feeding cells (giant cells; GCs) inside a pseudo-organ (gall) from still unknown root cells. Understanding GCs ontogeny is essential to the basic knowledge of RKN-plant interaction and to discover novel and effective control strategies. Hence, we report for the first time in a model plant, Arabidopsis, molecular, and cellular features concerning ectopic de novo organogenesis of RKNs GCs in leaves. RKNs induce GCs in leaves with irregular shape, a reticulated cytosol, and fragmented vacuoles as GCs from roots...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28578872/an-atlas-of-%C3%AE-glucuronidases-in-the-human-intestinal-microbiome
#10
Rebecca M Pollet, Emma H D'Agostino, William G Walton, Yongmei Xu, Michael S Little, Kristen A Biernat, Samuel J Pellock, Loraine M Patterson, Benjamin C Creekmore, Hanna N Isenberg, Rohini R Bahethi, Aadra P Bhatt, Jian Liu, Raad Z Gharaibeh, Matthew R Redinbo
Microbiome-encoded β-glucuronidase (GUS) enzymes play important roles in human health by metabolizing drugs in the gastrointestinal (GI) tract. The numbers, types, and diversity of these proteins in the human GI microbiome, however, remain undefined. We present an atlas of GUS enzymes comprehensive for the Human Microbiome Project GI database. We identify 3,013 total and 279 unique microbiome-encoded GUS proteins clustered into six unique structural categories. We assign their taxonomy, assess cellular localization, reveal the inter-individual variability within the 139 individuals sampled, and discover 112 novel microbial GUS enzymes...
July 5, 2017: Structure
https://www.readbyqxmd.com/read/28555430/effect-of-gene-order-in-dna-constructs-on-gene-expression-upon-integration-into-plant-genome
#11
M Aydın Akbudak, Vibha Srivastava
Several plant biotechnology applications are based on the expression of multiple genes located on a single transformation vector. The principles of stable expression of foreign genes in plant cells include integration of full-length gene fragments consisting of promoter and transcription terminator sequences, and avoiding converging orientation of the gene transcriptional direction. Therefore, investigators usually generate constructs in which genes are assembled in the same orientation. However, no specific information is available on the effect of the order in which genes should be assembled in the construct to support optimum expression of each gene upon integration in the genome...
June 2017: 3 Biotech
https://www.readbyqxmd.com/read/28544311/impact-of-body-mass-index-on-robot-assisted-radical-cystectomy-with-intracorporeal-urinary-diversion
#12
Nariman Ahmadi, Thomas G Clifford, Gus Miranda, Jie Cai, Monish Aron, Mihir M Desai, Inderbir S Gill
OBJECTIVES: To determine the impact of body mass index (BMI) on peri-operative and oncological outcomes after robot-assisted radical cystectomy (RARC) with intracorporeal urinary diversion. PATIENTS AND METHODS: A total of 216 patients undergoing RARC, extended lymphadenectomy and intracorporeal urinary diversion, between July 2010 and December 2015, were categorized into four BMI groups according to the 2004 World Health Organization obesity classification groups: <25 kg/m(2) (normal); 25-29...
May 22, 2017: BJU International
https://www.readbyqxmd.com/read/28520714/alteration-of-stream-temperature-by-natural-and-artificial-beaver-dams
#13
Nicholas Weber, Nicolaas Bouwes, Michael M Pollock, Carol Volk, Joseph M Wheaton, Gus Wathen, Jacob Wirtz, Chris E Jordan
Beaver are an integral component of hydrologic, geomorphic, and biotic processes within North American stream systems, and their propensity to build dams alters stream and riparian structure and function to the benefit of many aquatic and terrestrial species. Recognizing this, beaver relocation efforts and/or application of structures designed to mimic the function of beaver dams are increasingly being utilized as effective and cost-efficient stream and riparian restoration approaches. Despite these verities, the notion that beaver dams negatively impact stream habitat remains common, specifically the assumption that beaver dams increase stream temperatures during summer to the detriment of sensitive biota such as salmonids...
2017: PloS One
https://www.readbyqxmd.com/read/28505352/the-nodulin-26-like-intrinsic-membrane-protein-osnip3-2-is-involved-in-arsenite-uptake-by-lateral-roots-in-rice
#14
Yi Chen, Sheng-Kai Sun, Zhong Tang, Guidong Liu, Katie L Moore, Frans J M Maathuis, Antony J Miller, Steve P McGrath, Fang-Jie Zhao
Previous studies have shown that the Nodulin 26-like intrinsic membrane protein (NIP) Lsi1 (OsNIP2;1) is involved in arsenite [As(III)] uptake in rice (Oryza sativa). However, the role of other rice NIPs in As(III) accumulation in planta remains unknown. In the present study, we investigated the role OsNIP3;2 in As(III) uptake in rice. When expressed in Xenopus laevis oocytes, OsNIP3;2 showed a high transport activity for As(III). Quantitative real-time RT-PCR showed that the expression of OsNIP3;2 was suppressed by 5 µM As(III), but enhanced by 20 and 100 µM As(III)...
May 17, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28462487/psychopathology-and-friendship-in-children-and-adolescents-disentangling-the-role-of-co-occurring-symptom-domains-with-serial-mediation-models
#15
Arthur Gus Manfro, Pedro M Pan, Ary Gadelha, Marcelo Fleck, Maria C do Rosário, Hugo Cogo-Moreira, Rodrigo Affonseca-Bressan, Jair Mari, Euripedes C Miguel, Luis A Rohde, Giovanni A Salum
The consolidation of social friendship groups is a vital part of human development. The objective of this study is to understand the direct and indirect influences of three major symptomatic domains-emotional, hyperkinetic, and conduct-on friendship. Specifically, we aim to study if the associations of one domain with friendship may be mediated by co-occurring symptoms from another domain. A total of 2512 subjects aged 6-14 years participated in this study. Friendship was evaluated by the Development and Well-Being Assessment's friendship section...
May 2, 2017: European Child & Adolescent Psychiatry
https://www.readbyqxmd.com/read/28430019/characterization-of-rph24-a-gene-conferring-adult-plant-resistance-to-puccinia-hordei-in-barley
#16
Laura A Ziems, Lee T Hickey, Gregory J Platz, Jerome D Franckowiak, Peter M Dracatos, Davinder Singh, Robert F Park
We identified Rph24 as a locus in barley (Hordeum vulgare L.) controlling adult plant resistance (APR) to leaf rust, caused by Puccinia hordei. The locus was previously reported as a quantitative trait locus in barley line ND24260-1 and named qRphND. We crossed ND24260-1 to the leaf-rust-susceptible standard Gus and determined inheritance patterns in the progeny. For the comparative marker frequency analysis (MFA), resistant and susceptible tails of the F2 were genotyped with Diversity Arrays Technology genotyping-by-sequencing (DArT-Seq) markers...
July 2017: Phytopathology
https://www.readbyqxmd.com/read/28392226/the-relationship-of-plasma-creatinine-as-egfr-and-high-sensitivity-cardiac-troponin-and-nt-probnp-concentrations-in-a-hospital-and-community-outpatient-population
#17
Julia M Potter, Aaron J Simpson, Jennifer Kerrigan, Emma Southcott, Marie M Salib, Gus Koerbin, Peter E Hickman
OBJECTIVES: While persons with overt renal failure have a well-described rise in troponin and NT-proBNP, it is less well described what the relationship is between cardiac markers and persons with impaired renal function, not requiring dialysis. DESIGN & METHODS: We have collected ALL samples referred to our pathology practice over a 24h period and measured hs-cTnI, hs-cTnT, NT-proBNP, calculated the eGFR, and related our measurements to clinical outcomes. RESULTS: For both men and women, for all of hs-cTnI, hs-cTnT and NT-proBNP, there was a graded response, as renal function worsened, the concentration of the cardiac marker increased...
October 2017: Clinical Biochemistry
https://www.readbyqxmd.com/read/28287543/modified-drop-tower-impact-tests-for-american-football-helmets
#18
G Alston Rush, R Prabhu, Gus A Rush, Lakiesha N Williams, M F Horstemeyer
A modified National Operating Committee on Standards for Athletic Equipment (NOCSAE) test method for American football helmet drop impact test standards is presented that would provide better assessment of a helmet's on-field impact performance by including a faceguard on the helmet. In this study, a merger of faceguard and helmet test standards is proposed. The need for a more robust systematic approach to football helmet testing procedures is emphasized by comparing representative results of the Head Injury Criterion (HIC), Severity Index (SI), and peak acceleration values for different helmets at different helmet locations under modified NOCSAE standard drop tower tests...
February 19, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28265995/analyzing-cytokinin-responses-during-plant-nematode-interactions
#19
Florian M W Grundler, Shahid Siddique
Cyst nematodes are obligate biotrophs that induce the formation of a hypertrophied and hypermetabolic syncytial-feeding site in roots of the host plants. Cytokinin signaling is activated at the site of infection and contributes significantly to the formation of syncytium. Here, we describe a protocol for visualizing cytokinin signaling activation in Arabidopsis upon infection with cyst nematode Heterodera schachtii using promoter reporter lines, TCSn:GFP and ARR5:GUS.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28263716/statistical-considerations-for-determining-high-sensitivity-cardiac-troponin-reference-intervals
#20
MULTICENTER STUDY
Peter E Hickman, Gus Koerbin, Julia M Potter, Walter P Abhayaratna
The troponin 99th percentile is used as the laboratory decision point in the diagnosis of acute myocardial infarction. A recent publication has shown that the statistical treatment for outlier removal may dramatically change the calculated troponin 99th percentile. We have used our large database from the previously reported Canberra Heart Study to independently assess the effect of various methods for removing outliers on the calculated 99th percentile. We have performed the same exercise using the troponin 97...
June 2017: Clinical Biochemistry
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