keyword
MENU ▼
Read by QxMD icon Read
search

Pbmc

keyword
https://www.readbyqxmd.com/read/29245183/increased-expression-and-modulated-regulatory-activity-of-co-inhibitory-receptors-pd-1-tigit-and-tim-3-in-lymphocytes-of-systemic-sclerosis-patients
#1
Michelle Fleury, Anna C Belkina, Elizabeth A Proctor, Christopher Zammitti, Robert W Simms, Douglas A Lauffenburger, Jennifer E Snyder-Cappione, Robert Lafyatis, Hans Dooms
OBJECTIVE: Immune dysfunction is an important component of the disease process underlying Systemic Sclerosis (SSc), but the mechanisms contributing to altered immune cell function in SSc remain poorly defined. Here, we measured the expression and function of the co-inhibitory receptors (co-IRs) PD-1, TIGIT, TIM-3, and LAG-3 in lymphocyte subsets from the peripheral blood of SSc patients. METHODS: Co-IR expression levels on immune cell subsets were analyzed using a 16-color flow cytometry panel...
December 15, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29241838/telomerase-level-increase-is-related-to-n-3-polyunsaturated-fatty-acid-efficacy-in-first-episode-schizophrenia-secondary-outcome-analysis-of-the-offer-randomized-clinical-trial
#2
Tomasz Pawełczyk, Marta Grancow-Grabka, Elżbieta Trafalska, Janusz Szemraj, Natalia Żurner, Agnieszka Pawełczyk
Schizophrenia is associated with shortening of the lifespan mainly due to cardiovascular events, cancer and chronic obstructive pulmonary disease. Both telomere attrition and decrease of telomerase levels were observed in schizophrenia. Polyunsaturated fatty acids (PUFA) influence multiple biochemical mechanisms which are postulated to accelerate telomere shortening and limit the longevity of patients with schizophrenia. Intervention studies based on add-on therapy with n-3 polyunsaturated fatty acids (n-3 PUFA) in patients with schizophrenia did not assess the changes in telomerase levels...
December 11, 2017: Progress in Neuro-psychopharmacology & Biological Psychiatry
https://www.readbyqxmd.com/read/29241030/expression-profiles-of-genes-involved-in-tlrs-and-nlrs-signaling-pathways-of-water-buffaloes-infected-with-fasciola-gigantica
#3
Fu-Kai Zhang, Jun-Ling Hou, Ai-Jiang Guo, Ai-Ling Tian, Zhao-An Sheng, Wen-Bin Zheng, Wei-Yi Huang, Hany M Elsheikha, Xing-Quan Zhu
Infection of ruminants and humans with Fasciola gigantica is attracting increasing attention due to its economic impact and public health significance. However, little is known of innate immune responses during F. gigantica infection. Here, we investigated the expression profiles of genes involved in Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways in buffaloes infected with 500F. gigantica metacercariae. Serum, liver and peripheral blood mononuclear cell (PBMC) samples were collected from infected and control buffaloes at 3, 10, 28, and 70days post infection (dpi)...
December 11, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29239690/antibody-drug-conjugates-design-and-development-for-therapy-and-imaging-in-and-beyond-cancer-labex-mabimprove-industrial-workshop-july-27-28-2017-tours-france
#4
Camille Martin, Claire Kizlik-Masson, André Pèlegrin, Hervé Watier, Marie-Claude Viaud-Massuard, Nicolas Joubert
The annual "Antibody Industrial Symposium", co organized by LabEx MAbImprove, MabDesign and Polepharma, was held in Tours, France on June 27-28, 2017. The focus was on antibody-drug-conjugates (ADCs), new entities which realize the hope of Paul Ehrlich's magic bullet. ADCs result from the bioconjugation of a highly cytotoxic drug to a selective monoclonal antibody, which acts as a vector. Building on knowledge gained during the development of three approved ADCs, brentuximab vedotin (Adcetris®), ado trastuzumab emtansine (Kadcyla®) and inotuzumab ozogamicin (Besponsa®), and the many ADCs in development, this meeting addressed strategies and the latest innovations in the field from fundamental research to manufacturing...
December 14, 2017: MAbs
https://www.readbyqxmd.com/read/29237833/neutralizing-activity-of-broadly-neutralizing-anti-hiv-1-antibodies-against-clade-b-clinical-isolates-produced-in-peripheral-blood-mononuclear-cells
#5
Yehuda Z Cohen, Julio C C Lorenzi, Michael S Seaman, Lilian Nogueira, Till Schoofs, Lisa Krassnig, Allison Butler, Katrina Millard, Tomas Fitzsimons, Xiaoju Daniell, Juan P Dizon, Irina Shimeliovich, David C Montefiori, Marina Caskey, Michel C Nussenzweig
Recently discovered broadly neutralizing anti-HIV-1 antibodies (bNAbs) demonstrate extensive breadth and potency against diverse HIV-1 strains, and represent a promising approach for the treatment and prevention of HIV-1 infection. The breadth and potency of these antibodies have primarily been evaluated using panels of HIV-1 Env-pseudotyped viruses produced in 293T cells expressing molecularly cloned Envs. Here we report on the ability of 5 bNAbs currently in clinical development to neutralize circulating primary HIV-1 isolates derived from peripheral blood mononuclear cells (PBMC), and compare the results to the pseudovirus panels used to characterize the bNAbs...
December 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29237049/the-comet-assay-in-human-biomonitoring-cryopreservation-of-whole-blood-and-comparison-with-isolated-mononuclear-cells
#6
Gudrun Koppen, Sofie De Prins, An Jacobs, Vera Nelen, Greet Schoeters, Sabine A S Langie
The comet assay is often applied in human biomonitoring. Most of the time the assay is performed with isolated peripheral blood mononuclear cells (PBMC). However, using whole blood instead of isolated cells reduces processing time, and only 20 µl is sufficient for analysis. In this study, a cryopreservation protocol for human whole blood for application in the comet assay was optimised by removing excess plasma before adding freezing medium. Cryopreservation of whole blood samples (n = 30) did not increase the detected level of strand breaks and formamidopyrimidine DNA glycosylase (FPG)-sensitive sites...
December 9, 2017: Mutagenesis
https://www.readbyqxmd.com/read/29236770/association-between-preoperative-peripheral-blood-mononuclear-cell-gene-expression-profiles-early-postoperative-organ-function-recovery-potential-and-long-term-survival-in-advanced-heart-failure-patients-undergoing-mechanical-circulatory-support
#7
Galyna Bondar, Ryan Togashi, Martin Cadeiras, Joanna Schaenman, Richard K Cheng, Lindsay Masukawa, Josephine Hai, Tra-Mi Bao, Desai Chu, Eleanor Chang, Maral Bakir, Sophie Kupiec-Weglinski, Victoria Groysberg, Tristan Grogan, Joseph Meltzer, Murray Kwon, Maura Rossetti, David Elashoff, Elaine Reed, Pei Pei Ping, Mario C Deng
BACKGROUND: Multiorgan dysfunction syndrome contributes to adverse outcomes in advanced heart failure (AdHF) patients after mechanical circulatory support (MCS) implantation and is associated with aberrant leukocyte activity. We tested the hypothesis that preoperative peripheral blood mononuclear cell (PBMC) gene expression profiles (GEP) can predict early postoperative improvement or non-improvement in patients undergoing MCS implantation. We believe this information may be useful in developing prognostic biomarkers...
2017: PloS One
https://www.readbyqxmd.com/read/29236750/identification-of-gad65-aa-114-122-reactive-memory-like-nk-cells-in-newly-diagnosed-type-1-diabetic-patients-by-hla-class-i-pentamers
#8
Valentina Perri, Elena Gianchecchi, Loredana Cifaldi, Marsha Pellegrino, Ezio Giorda, Marco Andreani, Marco Cappa, Alessandra Fierabracci
Type 1 diabetes is an autoimmune disease, in which pancreatic β cells are destroyed by autoreactive T cells in genetically predisposed individuals. Serum beta cell autoantibody specificities have represented the mainstay for classifying diabetes as autoimmune-mediated and for stratifying risk in first-degree relatives. In recent years, approaches were attempted to solve the difficult issue of detecting rare antigen-specific autoreactive T cells and their significance to etiopathogenesis such as the use of the MHC multimer technology...
2017: PloS One
https://www.readbyqxmd.com/read/29236355/b-cell-specific-protein-fcrla-is-expressed-by-plasmacytoid-dendritic-cells-in-humans
#9
Evdokiya Reshetnikova, Sergey Guselnikov, Olga Volkova, Konstantin Baranov, Alexander Taranin, Ludmila Mechetina
BACKGROUND: Fc receptor-like A (FCRLA) is a unique member of a family of Fc receptor like-molecules that lacks a transmembrane region and is an ER-resident protein. In mice and humans, FCRLA has been known as a B cell specific protein. We report here that, in humans, FCRLA is also expressed in a subpopulation of plasmacytoid dendritic cells (pDCs). METHODS: Human peripheral blood mononuclear cells (PBMC), splenocytes, and tonsillar cells were stained for lineage markers followed by fixation/saponin permeabilization and intracellular staining for FCRLA, and then analyzed by flow cytometry with CD123 and CD303 used as pDC markers...
December 13, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29232525/a-modified-ficoll-paque-gradient-method-for-isolating-mononuclear-cells-from-the-peripheral-and-umbilical-cord-blood-of-humans-for-biobanks-and-clinical-laboratories
#10
Yanjuan Jia, Hui Xu, Yonghong Li, Chaojun Wei, Rui Guo, Fang Wang, Yu Wu, Jing Liu, Jing Jia, Junwen Yan, Xiaoming Qi, Yuanting Li, Xiaoling Gao
Although the Ficoll-Paque method is classically used to isolate peripheral blood mononuclear cells (PBMCs), modifications in this method are required for a more rapid and economic output for biobanks and clinical laboratories, particularly in developing countries. In this study, we addressed this issue by modifying the Ficoll-Paque method for the isolation of PBMCs or mononuclear cells from the peripheral and the umbilical cord blood of healthy and diseased (infected, anemic, and chronic obstructive pulmonary disease) adult individuals...
December 12, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/29227717/quercetin-restores-corticosteroid-sensitivity-in-cells-from-patients-with-chronic-obstructive-pulmonary-disease
#11
Akihisa Mitani, Aishah Azam, Chaitanya Vuppusetty, Kazuhiro Ito, Nicolas Mercado, Peter J Barnes
Corticosteroid resistance is a major barrier to the effective treatment of chronic obstructive pulmonary disease (COPD). Oxidative stress from cigarette smoke and chronic inflammation is likely to induce this corticosteroid insensitivity. Quercetin is a polyphenol that has been reported to be an active oxygen scavenger as well as a functional adenosine monophosphate-activated protein kinase (AMPK) activator. The aim of this study was to investigate the effect of quercetin on corticosteroid responsiveness in COPD cells...
December 11, 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/29227276/alteration-in-the-cytokine-secretion-pattern-in-t-cells-of-patients-with-cystic-fibrosis-caused-by-dna-methyltransferase-inhibitor-5-azacitidine
#12
E Kvaratskhelia, N Dabrundashvili, M Gagua, E Maisuradze, M Kamkamidze, E Abzianidze
Cystic fibrosis (CF) is the autosomal-recessive disorder caused by mutation in the cystic fibrosis transmembrane conductance regulator gene (CFTR). The Airway inflammation plays a central role in the progression of CF disease. Cystic fibrosis characterized by the overproduction of the pro-inflammatory cytokines and reduced expression of anti-inflammatory cytokines. Although the mechanisms of abnormal cytokine expression is still poorly understood, altered epigenetic regulations in T cells might contribute. In the present study we examined the expression of IFN-γ and IL-10 by CF T cells prior to and following 5-azaC treatment...
November 2017: Georgian Medical News
https://www.readbyqxmd.com/read/29226636/the-value-of-gpc3-and-gp73-in-clinical-diagnosis-of-hepatocellular-carcinoma
#13
Ji-Sheng Jing, Wei Ye, Ying-Kui Jiang, Jie Ma, Meng-Qi Zhu, Jiu-Ming Ma, Hua Zhou, La-Qing Yu, Yong-Feng Yang, Shun-Cai Wang
BACKGROUND: The incidence of hepatocellular carcinoma (HCC) has increased over the past decades in China. Current screening methods of HCC such as detection of α-fetoprotein (AFP) combined with liver ultrasonography remain unsatisfactory. Many HCC patients have already missed the optimal treatment period when diagnosed. Our study aimed to evaluate the value of Glypican 3 (GPC3) and Golgi protein 73 (GP73) in the detection of HCC. METHODS: Thirty-nine patients with HCC and 31 patients with liver cirrhosis were enrolled...
November 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/29226098/occult-hcv-infection-oci-diagnosis-in-cirrhotic-and-non-cirrhotic-na%C3%A3-ve-patients-by-intra-pbmc-nested-viral-rna-pcr
#14
Mohamed Darwish Ahmed Abd Alla, Saleh Ahmed Elibiary, George Y Wu, Mostafa Kamel El-Awady
Background and Aims: Occult HCV infections (OCIs) include IgG antibody seronegative cryptogenic (COCIs), as well as seropositive secondary naïve (SNOCIs) and experienced (SEOCIs) cases. We used peripheral-blood-mononuclear-cell (PBMC)-PCR to evaluate COCIs and SNOCIs prevalence, serum HCV spontaneous disappearance (SCSD) in naïve cirrhotics and non-cirrhotics, intra-PBMC HCV-RNA strands in relation to cirrhosis density in naïve non-viremia cases, and HCV-RNA seroconversion after 1 year of solitary naïve intra-PBMC infection...
December 28, 2017: Journal of Clinical and Translational Hepatology
https://www.readbyqxmd.com/read/29225331/elevated-peripheral-expression-of-neuregulin-1-nrg1-mrna-isoforms-in-clozapine-treated-schizophrenia-patients
#15
Md Shaki Mostaid, Ting Ting Lee, Gursharan Chana, Suresh Sundram, Cynthia Shannon Weickert, Christos Pantelis, Ian Everall, Chad Bousman
Differential expression of neuregulin-1 (NRG1) mRNA isoforms and proteins has been reported in schizophrenia, primarily in post-mortem brain tissue. In this study, we examined 12 NRG1 SNPs, eight NRG1 mRNA isoforms (type I, type I(Ig2), type II, type III, type IV, EGFα, EGFβ, pan-NRG1) in whole blood, and NRG1-β1 protein in serum of clozapine-treated schizophrenia patients (N = 71) and healthy controls (N = 57). In addition, using cultured peripheral blood mononuclear cells (PBMC) from 15 healthy individuals, we examined the effect of clozapine on NRG1 mRNA isoform and protein expression...
December 11, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/29225295/effect-of-smoking-status-on-monocyte-tissue-factor-activity-carotid-atherosclerosis-and-long-term-prognosis-in-metabolic-syndrome
#16
Keiichi Kohashi, Akihiro Nakagomi, Taichirou Morisawa, Ikuko Endoh, Naomi Kawaguchi, Yoshiki Kusama, Wataru Shimizu
BACKGROUND: Smoking increases the risk of atherothrombotic events. Tissue factor (TF) mainly expressed on monocytes plays an important role in thrombosis and atherosclerosis. Metabolic syndrome (MetS) is being increasingly recognized as a major atherothrombotic risk factor, but the effects of smoking on monocyte TF activity (MTFA), carotid atherosclerosis estimated on carotid intima-media thickness (CIMT), and long-term prognosis in MetS remain unclear.Methods and Results:A total of 301 MetS patients lacking any known cardiovascular disease were prospectively investigated and classified into 4 groups according to smoking status at entry and at 12 months as follows: never smokers, past smokers, quitters, and persistent smokers...
December 9, 2017: Circulation Journal: Official Journal of the Japanese Circulation Society
https://www.readbyqxmd.com/read/29225136/in-vitro-apoptotic-mechanism-of-a-novel-synthetic-quinazolinyl-derivative-induces-caspase-dependent-intrinsic-pathway-on-thp-1-leukemia-cell-line
#17
V Sridhar, S K Arepalli, L R Velatooru, J Venkateswara Rao, P Kavin Kennedy, B Narsaiah
Several quinazoline derivatives have been found to possess a broad spectrum of biological activities. Previously our research group has synthesized and studied the anti-proliferative effects of N-Decyl-N-(2-Methyl-4-Quinazolinyl) Amine (DMQA). The current study evaluated the cytotoxic and apoptotic properties of DMQA in THP-1 cells. The cytotoxic potential of DMQA was assessed using MTT assay on a panel of cancer cell lines which include HeLa, Mia PaCa-2, A 375, B16-F10, A 549,A 431, U937, THP-1, HL-60 and peripheral blood mononuclear cells (PBMC's)...
December 7, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29223571/noval-nitroaromatic-compound-activates-autophagy-and-apoptosis-pathways-in-hl60%C3%A2-cells
#18
Gabriele Mendes M C Perdigão, Marcela Silva Lopes, Lucas Bonfim Marques, Pedro Henrique Dias Moura Prazeres, Kamila de Sousa Gomes, Renata Barbosa de Oliveira, Mauro Cunha Xavier Pinto, Elaine Maria de Souza-Fagundes
N-(2-butanoyloxyethyl)-4-(chloromethyl)-3-nitrobenzamide (NBCN) is a nitroaromatic bioreductible compound with cytotoxic effects in cancer cells lines. The aim of this work was investigated the molecular mechanisms involved in cell death promoted by NBCN on HL60 cells line. We observed that NBCN treatment increased intracellular ROS, reduced mitochondria membrane potential (ΔΨm). The treatment also induced morphological changes, phosphatidylserine exposure, cell cycle arrest in G2/M-phase, DNA condensation and fragmentation in HL60 cells, but did not showed cytotoxic effects on normal human peripheral blood mononuclear cells (PBMC)...
December 6, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29221433/hyporesponsiveness-of-natural-killer-cells-and-impaired-inflammatory-responses-in-critically-ill-patients
#19
Minkyung Kim, Minjoo Kim, Hana Jeong, Jey Sook Chae, Young Sam Kim, Jae Gil Lee, Younsoo Cho, Jong Ho Lee
BACKGROUND: To investigate natural killer (NK) cell activity, circulating cytokine level and peripheral blood mononuclear cell (PBMC) cytokine production status in critically ill patients. METHODS: Blood samples were collected <24 h after admission from 24 intensive care unit (ICU) patients and 24 age-, sex-, and body mass index (BMI)-matched healthy controls. Serum cytokine concentrations and cytokine production by PBMCs and lipopolysaccharide (LPS)-stimulated PBMCs were measured...
December 8, 2017: BMC Immunology
https://www.readbyqxmd.com/read/29218794/examining-the-potential-for-porcine-derived-islet-cells-to-harbour-viral-pathogens
#20
Claire Crossan, Zoe O'Hara, Nizar Mourad, Pierre Gianello, Linda Scobie
With an onus on safety in the potential use of porcine islet cells as a treatment for diabetes, the use of animals lacking exogenous pathogens is clearly important and multilevel screening strategies have been presented on testing animals and the product. In this study, we wished to investigate whether islet cells indeed harboured the same viral pathogens of concern in the source animal. PMBC and islet cells from both adult and neonatal source animals were directly compared and tested for PCMV, PLHV, PCV2, PPV and HEV using both molecular and serological assays...
December 7, 2017: Xenotransplantation
keyword
keyword
10388
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"