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https://www.readbyqxmd.com/read/29656550/mir-181c-brk1-axis-plays-a-key-role-in-actin-cytoskeleton-dependent-t-cell-function
#1
Shok Ping Lim, Nikolaos Ioannou, Alan G Ramsay, David Darling, Joop Gäken, Ghulam J Mufti
MicroRNAs are short endogenous noncoding RNAs that play pivotal roles in a diverse range of cellular processes. The miR-181 family is important in T cell development, proliferation, and activation. In this study, we have identified BRK1 as a potential target of miR-181c using a dual selection functional assay and have showed that miR-181c regulates BRK1 by translational inhibition. Given the importance of miR-181 in T cell function and the potential role of BRK1 in the involvement of WAVE2 complex and actin polymerization in T cells, we therefore investigated the influence of miR-181c-BRK1 axis in T cell function...
April 14, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29656374/expression-of-circulating-semaphorin3a-and-its-association-with-inflammation-and-bone-destruction-in-rheumatoid-arthritis
#2
Hui Gao, Xiao-Xu Ma, Qian Guo, Lin-Feng Xie, Yu-Chao Zhong, Xue-Wu Zhang
To determine the expression of Semaphorin3A (Sema3A) in rheumatoid arthritis (RA) patients, and analyze the correlation between serum Sema3A and the pathogenesis of RA. The concentration of serum Sema3A and its mRNA expression level were detected in RA patients. The association of serum Sema3A level with clinical and laboratory features of RA were analyzed. Serum Sema3A of 130 RA patients (15.89 ± 8.58 ng/ml) was significantly higher than that of 150 HC (6.96 ± 2.62 ng/ml) and 215 patients with other rheumatic diseases (P < 0...
April 14, 2018: Clinical Rheumatology
https://www.readbyqxmd.com/read/29656112/influence-of-immunosuppressive-drugs-on-the-cd30-molecule-in-kidney-transplanted-patients
#3
Patricia Cristina Grenzi, Érika Fernandes Campos, Hélio Tedesco-Silva, Claudia Rosso Felipe, Maria Fernanda Soares, José Medina-Pestana, Hinrich Peter Hansen, Maria Gerbase-DeLima
BACKGROUND: Soluble CD30 (sCD30) is a suggested marker for kidney transplantation outcomes. We investigated whether sCD30 serum levels are influenced by immunosuppression and whether they correlate with findings in protocol biopsies and with CD30 gene expression in peripheral blood mononuclear cells (PBMC). METHODS: We studied 118 kidney transplant recipients that initially received tacrolimus (TAC) and, at month-3, were converted or not to sirolimus (SRL). RESULTS: sCD30 serum levels gradually declined after transplantation, being the decline more pronounced in the SRL group...
April 12, 2018: Human Immunology
https://www.readbyqxmd.com/read/29655569/activation-of-the-porcine-alveolar-macrophages-via-toll-like-receptor-4-nf-%C3%AE%C2%BAb-mediated-pathway-provides-a-mechanism-of-resistin-leading-to-inflammation
#4
Bi Li, Jing Fang, Zhicai Zuo, Sirui Yin, Tingting He, Mingxian Yang, Junliang Deng, Liuhong Shen, Xiaoping Ma, Shumin Yu, Ya Wang, Zhihua Ren, Hengmin Cui
Resistin, a previously discovered cysteine-rich adipokine known to regulate glucose metabolism, has been emerged as a mediator in inflammation and immunity. Its level was supposed to be related to the expression of indicators, such as interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) in inflammation. Toll-like receptor 4 (TLR4) was reported to be a receptor for resistin in cells, like leukocytes and peripheral blood mononuclear cells (PBMC). However, the pro-inflammatory role of resistin and its intracellular mechanisms in alveolar macrophages have not been thoroughly validated...
April 11, 2018: Cytokine
https://www.readbyqxmd.com/read/29651447/impaired-gal-9-dysregulates-the-pbmc-induced-th1-th2-imbalance-in-abortion-prone-matings
#5
Mengzhou He, Ming Jiang, Yuan Zhou, Fanfan Li, Meitao Yang, Yao Fan, Yin Xie, Rajluxmee Beejadhursing, Ling Feng, Dongrui Deng
Recurrent miscarriage is defined as the loss of 3 or more consecutive pregnancies; however, the underlying immunologic mechanisms that trigger pregnancy loss remain largely unelucidated. Galectin-9 (Gal-9) may modulate a variety of biologic functions and play an important role in Th1/Th2 immune deviation. To analyze the mechanism of Gal-9 in abortion, we used the classical abortion-prone mouse model (DBA/2-mated CBA/J mice) to detect the expression of Gal-9 at the maternal-fetal interface. We also mimicked the immune environment of pregnancy by culturing trophoblast cells with peripheral blood mononuclear cells (PBMCs) to explore how Gal-9 might be involved in the pathogenesis of abortion...
2018: Journal of Immunology Research
https://www.readbyqxmd.com/read/29649425/effects-of-grape-consumption-on-biomarkers-of-inflammation-endothelial-function-and-pbmc-gene-expression-in-obese-subjects
#6
Allison S Bardagjy, Qian Hu, Kathryn A Giebler, Addison Ford, Francene M Steinberg
This study investigated effects of grape consumption on biomarkers of cardiovascular health in obese participants in both postprandial and chronic settings. Twenty obese adults participated in this randomized, placebo controlled, double-blinded crossover trial. Participants were randomized to consume 60 g freeze-dried polyphenol-rich whole grape powder (GP) or placebo (PBO) followed by high fat high carbohydrate (HFHC) meal challenge. Following acute challenge, participants consumed their respective treatment daily for 4 weeks to determine effects of chronic consumption...
April 9, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29643246/the-dynamics-of-siv-2-ltr-circles-in-the-presence-and-absence-of-cd8-cells
#7
Benjamin B Policicchio, Erwing Fabian Cardozo, Paola Sette, Cuiling Xu, George Haret-Richter, Tammy Dunsmore, Cristian Apetrei, Ivona Pandrea, Ruy M Ribeiro
CD8+ cells play a key role in HIV/SIV infection, but their specific mechanism(s) of action in controlling the virus are unclear. 2-LTR circles are extrachromosomal products generated upon failed integration of HIV/SIV. To understand the specific effects of CD8+ cells on infected cells, we analyzed the dynamics of 2-LTR circles in SIVmac251-infected rhesus macaques (RM) treated with an integrase inhibitor (INT). Twenty RMs underwent CD8+ cell depletion, received RAL monotherapy or a combination of both. Blood, lymph nodes (LNs) and gut biopsies were routinely sampled...
April 11, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29643191/cd83-antibody-inhibits-human-b-cell-responses-to-antigen-as-well-as-dendritic-cell-mediated-cd4-t-cell-responses
#8
Kuan Y Wong, Rebecca Baron, Therese A Seldon, Martina L Jones, Alison M Rice, David J Munster
Anti-CD83 Ab capable of Ab-dependent cellular cytotoxicity can deplete activated CD83+ human dendritic cells, thereby inhibiting CD4 T cell-mediated acute graft-versus-host disease. As CD83 is also expressed on the surface of activated B lymphocytes, we hypothesized that anti-CD83 would also inhibit B cell responses to stimulation. We found that anti-CD83 inhibited total IgM and IgG production in vitro by allostimulated human PBMC. Also, Ag-specific Ab responses to immunization of SCID mice xenografted with human PBMC were inhibited by anti-CD83 treatment...
April 11, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29643001/immune-cell-bdnf-expression-in-treatment-na%C3%A3-ve-relapsing-remitting-multiple-sclerosis-patients-and-following-one-year-of-immunomodulation-therapy
#9
Alicja Kalinowska-Łyszczarz, Mikołaj A Pawlak, Aleksandra Wyciszkiewicz, Krystyna Osztynowicz, Wojciech Kozubski, Sławomir Michalak
Although neurons are the main source of neurotrophins in the healthy brain, neurotrophins can also be expressed in the immune system. We have previously shown that in relapsing-remitting multiple sclerosis (RRMS) lower immune-cell neurotrophin levels are associated with brain atrophy and cognitive impairment. The aim of the present study was to assess if immune-cell neurotrophin expression is impaired in MS as compared with the healthy controls, and to describe if these levels change in treatment-naïve RRMS patients, following one year of immunomodulation...
March 29, 2018: Neurologia i Neurochirurgia Polska
https://www.readbyqxmd.com/read/29628923/evaluation-of-antigen-conjugated-fluorescent-beads-to-identify-antigen-specific-b-cells
#10
Isabel Correa, Kristina M Ilieva, Silvia Crescioli, Sara Lombardi, Mariangela Figini, Anthony Cheung, James F Spicer, Andrew N J Tutt, Frank O Nestle, Panagiotis Karagiannis, Katie E Lacy, Sophia N Karagiannis
Selection of single antigen-specific B cells to identify their expressed antibodies is of considerable interest for evaluating human immune responses. Here, we present a method to identify single antibody-expressing cells using antigen-conjugated fluorescent beads. To establish this, we selected Folate Receptor alpha (FRα) as a model antigen and a mouse B cell line, expressing both the soluble and the membrane-bound forms of a human/mouse chimeric antibody (MOv18 IgG1) specific for FRα, as test antibody-expressing cells...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29627691/selective-cytotoxic-and-genotoxic-activities-of-5-2-bromo-5-methoxybenzylidene-thiazolidine-2-4-dione-against-nci-h292-human-lung-carcinoma-cells
#11
Maria do D Rodrigues, Priscila B G S Santiago, Karla M R Marques, Valéria R A Pereira, Maria C A B de Castro, Jeanne C L L Cantalice, Teresinha G da Silva, Mônica L Adam, Silene C do Nascimento, Julianna F C de Albuquerque, Gardenia C G Militao
BACKGROUND: Thiazolidine-2,4-dione ring system is used as a pharmacophore to build various heterocyclic compounds aimed to interact with biological targets. In the present study, benzylidene-2,4-thiazolidinedione derivatives (compounds 2-5) were synthesized and screened against cancer cell lines and the genotoxicity and cytotoxicity of the most active compound (5) was investigated on normal and lung cancer cell line. METHODS: For in vitro cytotoxic screening, the MTT assay was used for HL60 and K562 (leukemia), MCF-7 (breast adenocarcinoma), HT29 (colon adenocarcinoma), HEp-2 (cervix carcinoma) and NCI-H292 (lung carcinoma) tumor cell lines and Alamar-blue assay was used for non-tumor cells (PBMC, human peripheral blood mononuclear cells) were used...
November 22, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29626432/newcastle-disease-virus-strain-af2240-as-an-oncolytic-virus-a-review
#12
REVIEW
Jeevanathan Kalyanasundram, Aini Hamid, Khatijah Yusoff, Suet Lin Chia
The discovery of tumour selective virus-mediated apoptosis marked the birth of an alternative cancer treatment in the form of oncolytic viruses. Even though, its oncolytic efficiency was demonstrated more than 50 years ago, safety concerns which resulted from mild to lethal side effects hampered the progress of oncolytic virus research. Since the classical oncolytic virus studies rely heavily on its natural oncolytic ability, virus manipulation was limited, thereby, restricted efforts to improve its safety...
April 4, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29621898/curcumin-and-its-synthetic-analogue-dimethoxycurcumin-differentially-modulates-antioxidant-status-of-normal-human-peripheral-blood-mononuclear-cells
#13
Emmanuel Simon, P Aswini, V B Sameer Kumar, Gokuldas Mankadath
Curcumin is a polyphenol derived from the herb Curcuma longa, which has been extensively studied in terms of its antitumour, antioxidant, and chemopreventive activity as well as various other effects. In the present work we compared curcumin with its synthetic analogue dimethoxycurcumin (dimc) in terms of its antioxidant enzyme-modulating effects in human peripheral blood mononuclear cells (PBMC). We found that these compounds modulate antioxidant enzymes differentially. Both curcumin and dimethoxycurcumin effected a decrease in lipid peroxidation status in PBMC, however, curcumin had better activity in this regard...
April 6, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29621771/low-necroptosis-process-predicts-poor-treatment-outcome-of-human-papillomavirus-positive-cervical-cancers-by-decreasing-tumor-associated-macrophages-m1-polarization
#14
Lin Li, Song Yu, Chunyi Zang
BACKGROUND/AIM: The aim of this study was to assess the functions of the necroptosis process on the prognosis of high-risk human papillomavirus (HR-HPV)-related cervical cancer. METHODS: PCR and western blotting were used to demonstrate the expression of the necroptosis marker, mixed lineage kinase domain-like protein (MLKL), in whole blood and peripheral blood mononuclears (PBMCs) of 89 cervical cancer patients and 15 healthy volunteers. Necroptosis levels and M1 polarization were determined in tumor co-cultured macrophages...
April 5, 2018: Gynecologic and Obstetric Investigation
https://www.readbyqxmd.com/read/29621357/oncolytic-effect-of-wild-type-newcastle-disease-virus-isolates-in-cancer-cell-lines-in-vitro-and-in-vivo-on-xenograft-model
#15
Kseniya S Yurchenko, Peipei Zhou, Anna V Kovner, Evgenii L Zavjalov, Lidiya V Shestopalova, Alexander M Shestopalov
Oncolyic virotherapy is one of the modern experimental techniques to treat human cancers. Here we studied the antitumor activity of wild-type Newcastle disease virus (NDV) isolates from Russian migratory birds. We showed that NDV could selectively kill malignant cells without affecting healthy cells. We evaluated the oncolytic effect of 44 NDV isolates in 4 histogenetically different human cell lines (HCT116, HeLa, A549, MCF7). The safety of the isolates was also tested in normal peripheral blood mononuclear (PBMC) cells...
2018: PloS One
https://www.readbyqxmd.com/read/29621281/evidence-of-functional-cell-mediated-immune-responses-to-nontypeable-haemophilus-influenzae-in-otitis-prone-children
#16
Elke Seppanen, Dino Tan, Karli J Corscadden, Andrew J Currie, Peter C Richmond, Ruth B Thornton, Lea-Ann S Kirkham
Otitis media (OM) remains a common paediatric disease, despite advances in vaccinology. Susceptibility to recurrent acute OM (rAOM) has been postulated to involve defective cell-mediated immune responses to common otopathogenic bacteria. We compared the composition of peripheral blood mononuclear cells (PBMC) from 20 children with a history of rAOM (otitis-prone) and 20 healthy non-otitis-prone controls, and assessed innate and cell-mediated immune responses to the major otopathogen nontypeable Haemophilus influenzae (NTHi)...
2018: PloS One
https://www.readbyqxmd.com/read/29620199/correlation-between-autophagy-levels-in-peripheral-blood-mononuclear-cells-and-clinical-parameters-in-patients-with-chronic-obstructive-pulmonary-disease
#17
Yanjun Wu, Bo Xu, Xin He, Bo Wu, Yunxiao Li, Ganggang Yu, Chunting Tan, Haoyan Wang
Autophagy serves a role in the pathogenesis of chronic inflammatory diseases. The aim of the present study was to compare the autophagy levels in the peripheral blood mononuclear cells (PBMCs) of patients with chronic obstructive pulmonary disease (COPD) and healthy individuals and to assess the association between autophagy and the clinical parameters of COPD. Samples of peripheral blood from 20 patients with stable COPD and 20 healthy controls were collected. PBMCs were harvested using Ficoll density gradient centrifugation...
March 29, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29620176/%C3%AE-2%C3%A2-adrenergic-receptor-functionality-and-genotype-in-two-different-models-of-chronic-inflammatory-disease-liver-cirrhosis-and-osteoarthritis
#18
Reyes Roca, Pablo Esteban, Pedro Zapater, María-Del-Mar Inda, Anna Lucia Conte, Laura Gómez-Escolar, Helena Martínez, José F Horga, José M Palazon, Ana M Peiró
The present study was designed to investigate the functional status of β2 adrenoceptors (β2AR) in two models of chronic inflammatory disease: liver cirrhosis (LC) and osteoarthritis (OA). The β2AR gene contains three single nucleotide polymorphisms at amino acid positions 16, 27 and 164. The aim of the present study was to investigate the potential influence of lymphocyte β2AR receptor functionality and genotype in LC and OA patients. Blood samples from cirrhotic patients (n=52, hepatic venous pressure gradient 13±4 mmHg, CHILD 7±2 and MELD 11±4 scores), OA patients (n=30, 84% Kellgren‑Lawrence severity 4 grade, 14% knee replacement joint) and healthy volunteers as control group (n=26) were analyzed...
March 29, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29619756/tissue-specific-phenotype-and-activation-of-inkt-cells-in-morbidly-obese-subjects-interaction-with-adipocytes-and-effect-of-bariatric-surgery
#19
Soledad López, Sara García-Serrano, Carolina Gutierrez-Repiso, Francisca Rodríguez-Pacheco, Ailec Ho-Plagaro, Concepción Santiago-Fernandez, Gonzalo Alba, Marta Cejudo-Guillen, Alberto Rodríguez-Cañete, Sergio Valdes, Lourdes Garrido-Sanchez, David Pozo, Eduardo García-Fuentes
BACKGROUND: The immune response of visceral adipose tissue (VAT) in obesity, in particular the role of invariant natural killer T (iNKT) cells, has not yet been fully elucidated. OBJECTIVE: To characterize iNKT cells and its activation status in VAT and peripheral blood mononuclear cells (PBMC) in morbidly obese subjects (MO), and to analyze their association with metabolic parameters. SUBJECTS AND METHODS: Twenty non-obese and 20 MO subjects underwent Roux-en-Y gastric bypass (RYGB) and were studied before and 6 months after RYGB...
April 4, 2018: Obesity Surgery
https://www.readbyqxmd.com/read/29618666/peptide-vaccine-immunotherapy-biomarkers-and-response-patterns-in-pediatric-gliomas
#20
Sören Müller, Sameer Agnihotri, Karsen E Shoger, Max I Myers, Nicholas Smith, Srilakshmi Chaparala, Clarence R Villanueva, Ansuman Chattopadhyay, Adrian V Lee, Lisa H Butterfield, Aaron Diaz, Hideho Okada, Ian F Pollack, Gary Kohanbash
Low-grade gliomas (LGGs) are the most common brain tumor affecting children. We recently reported an early phase clinical trial of a peptide-based vaccine, which elicited consistent antigen-specific T cell responses in pediatric LGG patients. Additionally, we observed radiologic responses of stable disease (SD), partial response (PR), and near-complete/complete response (CR) following therapy. To identify biomarkers of clinical response in peripheral blood, we performed RNA sequencing on PBMC samples collected at multiple time points...
April 5, 2018: JCI Insight
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