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https://www.readbyqxmd.com/read/29053867/molecular-cloning-and-functional-analysis-of-peafowl-pavo-cristatus-toll-like-receptor-7
#1
H Song, M Zhang, W Gao, L Wu, G Li
In order to clone the peafowl (Pavo cristatus) Toll-like receptor 7 (TLR7) gene and study its biological function, the peafowl TLR7 coding sequences (CDS) were amplified by PCR of cDNA from the whole spleen of peafowl. The full-length sequence of the peafowl TLR7 gene CDS is 3,141 bp and encodes a 1,046-amino acid protein with a classic TLR composition of 16 leucine-rich repeats (LRR). Insertions of amino acids were found at position 15 of LRR2, LRR5, LRR7, LRR9, LRR11, LRR12, LRR14, and LRR15; and position 10 of LRR11...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29045131/dapsone-and-nitroso-dapsone-activation-of-na%C3%A3-ve-t-cells-from-healthy-donors
#2
Abdulaziz Alzahrani, Monday O Ogese, Xioali Meng, James Waddington, Arun Tailor, John Farrell, James L Maggs, Catherine J Betts, B Kevin Park, Dean John Naisbitt
Dapsone (DDS) causes hypersensitivity reactions in 0.5-3.6% of patients. Although clinical diagnosis is indicative of a hypersensitivity reaction, studies have not been performed to define whether dapsone or a metabolite activates specific T-cells. Thus, the aims of this study were to explore the immunogenicity DDS and nitroso DDS (DDS-NO) using PBMC from healthy donors and splenocytes from mice and generate human T-cell clones to characterize mechanisms of T-cell activation. DDS-NO was synthesized from DDS-hydroxylamine and shown to bind to the thiol group of glutathione and human and mouse albumin through sulfonamide and N-hydroxyl sulphonamide adducts...
October 18, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/29043425/arylamine-n-acetyltransferase-1-in-situ-n-acetylation-on-cd3-peripheral-blood-mononuclear-cells-correlate-with-natb-mrna-and-nat1-haplotype
#3
Raúl A Salazar-González, Eneida Turiján-Espinoza, David W Hein, Perla C Niño-Moreno, Silvia Romano-Moreno, Rosa C Milán-Segovia, Diana P Portales-Pérez
Human arylamine N-acetyltransferase 1 (NAT1) is responsible for the activation and elimination of xenobiotic compounds and carcinogens. Genetic polymorphisms in NAT1 modify both drug efficacy and toxicity. Previous studies have suggested a role for NAT1 in the development of several diseases. The aim of the present study was to evaluate NAT1 protein expression and in situ N-acetylation capacity in peripheral blood mononuclear cells (PBMC), as well as their possible associations with the expression of NAT1 transcript and NAT1 genotype...
October 17, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29042950/expression-of-ccr3-sox5-and-lc3-in-patients-with-elderly-onset-rheumatoid-arthritis-and-the-clinical-significance
#4
Jing Wang, Qing Zhao
This study investigated the expression of C-C chemokine receptor type 3 (CCR3), transcription factor SOX5 (SOX5) and microtubule-associated protein 1 light chain 3 (LC3) in patients with elderly onset rheumatoid arthritis (EORA) and the clinical significance. Ninety patients with elderly onset rheumatoid arthritis were selected in our hospital from January to December in 2016 to serve as patient group. At the same time, 50 healthy people were selected as control group. Levels of CCR3, SOX5 and LC3 in serum of two groups were detected by enzyme-linked immunosorbent assay (ELISA)...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29042255/comparison-of-raw264-7-human-whole-blood-and-pbmc-assays-to-screen-for-immunomodulators
#5
Ingrid Elisia, Han Bee Pae, Vivian Lam, Rachel Cederberg, Elyse Hofs, Gerald Krystal
The RAW264.7 mouse macrophage cell line is used extensively to carry out in vitro screens for immunomodulators. Compounds that are effective at reducing the expression of pro-inflammatory cytokines or nitric oxide (NO) from lipopolysaccharide (LPS)-stimulated RAW264.7 cells are often considered candidate anti-inflammatory agents for humans. There is, however, very little data on the reliability of this screen to identify bona fide human immunomodulators. We compared the efficacy of 37 purported immunomodulators to modulate LPS or E...
October 14, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/29040164/cd52-expression-on-cd4-t-cells-in-hiv-positive-individuals-on-cart
#6
Fie Juhl Vojdeman, Julie Christine Gaardbo, Hans Jakob Hartling, Marco Gelpi, Malene Hove-Skovsgaard, Anders Elm Pedersen, Susanne Dam Nielsen
BACKGROUND: Human immune defect virus (HIV) persists in a latent state in quiescent CD4+ T cells preventing eradication of HIV. CD52 is a surface molecule modulated by HIV. We aimed at examining factors related to CD52 expression on CD4+ T cells in HIV-positive individuals and the impact of initiation of combination antiretroviral therapy (cART). METHODS: Peripheral blood mononuclear cells (PBMC) from 18 HIV-positive individuals and 10 uninfected age and gender matched controls were examined by flow cytometry for CD38 and CD52 expression on CD4+ T cells...
October 14, 2017: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29038620/dengue-virus-induces-nk-cell-activation-through-trail-expression-during-infection
#7
Mariana Gandini, Fabienne Petitinga-Paiva, Cíntia Ferreira Marinho, Gladys Correa, Luzia Maria De Oliveira-Pinto, Luiz José de Souza, Rivaldo Venâncio Cunha, Claire Fernandes Kubelka, Elzinandes Leal de Azeredo
Dengue is an acute febrile illness with a wide spectrum of signs and symptoms ranging from mild to severe forms characterized by plasma leakage that can be fatal. NK cells are one of the main effectors in early infection and may play an important role in dengue pathogenesis. We investigated NK cell involvement during dengue infection. A higher frequency of NK cell subsets and TRAIL+NK cells was found in mild DF cases when compared to that in severe cases or healthy donors. NK activation markers such as CD107a and TLR3 were upregulated in patients' cells compared to those in healthy donors...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/29037951/design-and-synthesis-of-novel-nitrogen-mustard-evodiamine-hybrids-with-selective-antiproliferative-activity
#8
Xu Hu, Yan Wang, Jingjing Xue, Tong Han, Runwei Jiao, Zhanlin Li, Weiwei Liu, Fanxing Xu, Huiming Hua, Dahong Li
A series of novel nitrogen mustard-evodiamine hybrids were synthesized and evaluated for their antitproliferative properties. The antiproliferative activities of 10a-d, 11a-d, and 12a-d against four different kinds of human cancer cell lines (PC-3, HepG2, THP-1 and HL-60) and human normal peripheral blood mononuclear cells (PBMC) were determined. The results showed that all the target hybrid compounds exhibited antiproliferative activities against tested human tumor cell lines to some extent and no antiproliferative activities (>200 μM) against human normal PBMC cells...
October 7, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29035843/generation-of-a-human-control-pbmc-derived-ips-cell-line-tusmi001-a-from-a-healthy-male-donor-of-han-chinese-genetic-background
#9
Ying Wang, Yue Zhang, Jing Zhang, Jing Lu, Chaowen Yang, Jian Zhao, Gang Li, Zhongmin Liu, Ying Lei
A 59-year old healthy man of Han Chinese genetic background donated his peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system was used to reprogram his PBMCs with the human OSKM (Oct4, Sox2, Kl4 and c-Myc) transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype...
October 6, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29034892/generation-of-a-human-induced-pluripotent-stem-cell-line-from-a-65-year-old-healthy-female-donor-with-chinese-han-genetic-background
#10
Ying Wang, Jing Zhang, Yue Zhang, Danyi Huang, Jian Zhao, Gang Li, Ying Lei
Peripheral blood mononuclear cells (PBMC) were collected from a 65-year old healthy woman with Chinese Han genetic background. The PBMCs were reprogrammed with the human OKSM transcription factors using the non-integrating episomal vector system. The transgene-free iPSC showed pluripotency verified by immunocytochemistry for pluripotency markers and differentiated spontaneously toward the 3 germ layers in vitro. Furthermore, the iPSC line showed normal karyotype. The iPSC line can be used as control in disease mechanism studies...
October 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29034576/substantial-variability-across-individuals-in-the-vascular-and-nutrigenomic-response-to-an-acute-intake-of-curcumin-a-randomised-controlled-trial
#11
Nicolas Barber-Chamoux, Dragan Milenkovic, Marie-Anne Verny, Véronique Habauzit, Bruno Pereira, Céline Lambert, Damien Richard, Céline Boby, Andrzej Mazur, Jean René Lusson, Claude Dubray, Christine Morand
SCOPE: Curcumin exerts biological activities of interest in cardiovascular prevention. However, its vascular protective effect is still poorly investigated in humans. The present study aims to assess vasculat effect of an acute intake of curcumin and its nutrigenomic impact in circulating immune cells. METHODS AND RESULTS: In a randomised, double-blind, crossover design, eighteen healthy smokers consumed a placebo or a 5-gram of curcumin. Before and two hours after the intake, vascular function measurements were performed by using flow-mediated dilation (FMD)...
October 15, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29031635/correlation-of-altered-expression-of-a-long-non-coding-rna-neat1-in-peripheral-blood-mononuclear-cells-with-dengue-disease-progression
#12
Abhay Deep Pandey, Saptamita Goswami, Shweta Shukla, Shaoli Das, Suman Ghosal, Manisha Pal, Bhaswati Bandyopadhyay, Vishnampettai Ramachandran, Nandita Basu, Vikas Sood, Priyanka Pandey, Jayprokas Chakrabarti, Sudhanshu Vrati, Arup Banerjee
The association of long non-coding RNAs (lncRNAs) with dengue disease progression is currently unknown. Therefore, the present study aimed to identify lncRNAs in different categories of dengue patients and evaluate their association with dengue disease progression. Herein, we examined the expression profiles of lncRNAs and protein-coding genes between other febrile illness (OFI) and different grade of dengue patients through high-throughput RNA sequencing. We identified Nuclear Enriched Abundant Transcript 1 (NEAT1) as one of the differentially expressed lncRNAs (adjusted P ≤ 0...
October 12, 2017: Journal of Infection
https://www.readbyqxmd.com/read/29029878/dielectric-properties-of-plasma-membrane-a-signature-for-dyslipidemia-in-diabetes-mellitus
#13
Kakali Ghoshal, Subhadip Chakraborty, Chirantan Das, Sanatan Chattopadhyay, Subhankar Chowdhury, Maitree Bhattacharyya
Dielectric properties of a living biological membrane play crucial role indicating the status of the cell in pathogenic or healthy condition. A distinct variation in membrane capacitance and impedance was observed for PBMC suspensions for diabetic and diabetic-dyslipidemic subjects compared to healthy control. Low frequency region were explicitly considered in electrical analysis to address complex membrane dielectric factors that alter the system capacitance of a PBMC suspension. Such variation was marked in size, morphology and membrane function of PBMCs for control and diseased cases...
October 10, 2017: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29023581/activating-and-inhibitory-receptors-on-natural-killer-cells-in-patients-with-systemic-lupus-erythematosis-regulation-with-interleukin-15
#14
Syh-Jae Lin, Ming-Ling Kuo, Hsiu-Shan Hsiao, Pei-Tzu Lee, Ji-Yih Chen, Jing-Long Huang
Natural killer (NK) cells may play an important role in the pathogenesis of SLE. Interleukin(IL)-15, an NK-enhancing cytokine, is over-expressed in SLE patients. In the present study, we examined the effect of IL-15 on NK cytotoxicity of SLE patients, and the expression of various activating and inhibitory NK receptors on NK cells from SLE patients in relation to disease activity. We also sought to determine how IL-15 would affect the NK receptor expression on NK cells from SLE patients. PBMCs were collected from 88 SLE patients with inactive disease activity (SLEDAI score<6) and active disease activity (SLEDAI score≥6), 26 age-matched healthy adults were used as controls...
2017: PloS One
https://www.readbyqxmd.com/read/29017772/t-cell-activation-rho-gtpase-activating-protein-tagap-is-upregulated-in-clinical-and-experimental-arthritis
#15
Maria Arshad, Attya Bhatti, Peter John, Fazal Jalil, Federica Borghese, Joanna Z Kawalkowska, Richard O Williams, Felix I L Clanchy
Genome-wide association studies have identified various susceptibility variants and loci associated with incidence of rheumatoid arthritis (RA) in different populations. One of these is T cell activation Rho GTPase activating protein (TAGAP). The present study sought to measure the expression of TAGAP in RA patients, CD4(+) T cells subsets from healthy humans and in mice with collagen-induced arthritis. Peripheral blood mononuclear cells (PBMC) from RA patients and tissues of arthritic mice at different stages of the disease were used for the evaluation of TAGAP mRNA expression...
October 7, 2017: Cytokine
https://www.readbyqxmd.com/read/29017362/association-between-uncoupling-protein-2-adiponectin-and-resting-energy-expenditure-in-obese-women-with-normal-and-low-resting-energy-expenditure
#16
Zahra Taghadomi Masoumi, Mohammad Reza Eshraghian, Mahdi Hedayati, Hamideh Pishva
Obesity is recognized as the most prevalent metabolic disease worldwide. Decreases in energy expenditure may increase risk of obesity. One of the key regulators of energy balance is uncoupling protein2 (UCP2), a transporter protein presents in mitochondrial inner membrane. Moreover, adiponectin is the most abundant adipocytokine, it may play a role in energy metabolism and gene expression of UCP2. The aim of this study was to investigate potential associations between the level of uncoupling protein 2 and adiponectin and their relationship with REE (Resting Energy Expenditure) in obese women with normal and low resting energy expenditure...
October 10, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28993458/dairy-heifers-naturally-exposed-to-fasciola-hepatica-develop-a-type-2-immune-response-and-concomitant-suppression-of-leukocyte-proliferation
#17
John Graham-Brown, Catherine Hartley, Helen Clough, Aras Kadioglu, Matthew Baylis, Diana J L Williams
Fasciola hepatica is a parasitic trematode of global importance in livestock. Control strategies reliant on anthelmintics are unsustainable due to the emergence of drug resistance. Vaccines are under development, but efficacy is variable. Evidence from experimental infection suggest vaccine efficacy may be affected by parasite-induced immunomodulation. Little is known about the immune response to F. hepatica following natural exposure. Hence we analysed the immune responses over time in calves naturally exposed to F...
October 9, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28988038/innate-immune-transcriptomic-evaluation-of-pbmc-isolated-from-sheep-after-infection-with-e-ruminantium-welgevonden-strain
#18
T Nefefe, J Liebenberg, M van Kleef, H C Steyn, A Pretorius
Heartwater is a tick-borne non-infectious fatal disease of wild and domestic ruminants caused by the bacterium Ehrlichia ruminantium, transmitted by Amblyomma ticks. Although there is evidence that interferon-gamma (IFN-γ) controls E. ruminantium growth and that cellular immune responses could be protective, an effective recombinant vaccine for this disease is lacking. An overall analysis of which immune pathways are up- or down-regulated in sheep peripheral blood mononuclear cells is expected to lead to a better understanding of the global immune response of sheep to E...
October 4, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28986543/organs-on-chip-approach-a-tool-to-evaluate-cancer-immune-cells-interactions
#19
Elena Biselli, Elena Agliari, Adriano Barra, Francesca Romana Bertani, Annamaria Gerardino, Adele De Ninno, Arianna Mencattini, Davide Di Giuseppe, Fabrizio Mattei, Giovanna Schiavoni, Valeria Lucarini, Erika Vacchelli, Guido Kroemer, Corrado Di Natale, Eugenio Martinelli, Luca Businaro
In this paper we discuss the applicability of numerical descriptors and statistical physics concepts to characterize complex biological systems observed at microscopic level through organ on chip approach. To this end, we employ data collected on a microfluidic platform in which leukocytes can move through suitably built channels toward their target. Leukocyte behavior is recorded by standard time lapse imaging. In particular, we analyze three groups of human peripheral blood mononuclear cells (PBMC): heterozygous mutants (in which only one copy of the FPR1 gene is normal), homozygous mutants (in which both alleles encoding FPR1 are loss-of-function variants) and cells from 'wild type' donors (with normal expression of FPR1)...
October 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28986261/mmp-9-cleaved-osteopontin-isoform-mediates-tumor-immune-escape-by-inducing-expansion-of-myeloid-derived-suppressor-cells
#20
Lijuan Shao, Bo Zhang, Lingxiong Wang, Liangliang Wu, Quancheng Kan, Kexing Fan
As an extracellular matrix protein, osteopontin (OPN) has been shown to play an important role in regulation of the immune response to tumors. Myeloid-derived suppressor cells (MDSCs), a heterogeneous population of myeloid progenitors, are major components of the immune suppressive tumor microenvironment and contribute to tumor evasion of the immune response. However, the specific regulating mechanisms underlying MDSCs expansion remain unclear. Here, we found that MDSCs accumulated in the spleen and tumors of 3LL tumor-bearing mice...
October 4, 2017: Biochemical and Biophysical Research Communications
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