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https://www.readbyqxmd.com/read/28598479/organs-on-chips-with-integrated-electrodes-for-trans-epithelial-electrical-resistance-teer-measurements-of-human-epithelial-barrier-function
#1
Olivier Y F Henry, Remi Villenave, Michael J Cronce, William D Leineweber, Maximilian A Benz, Donald E Ingber
Trans-epithelial electrical resistance (TEER) is broadly used as an experimental readout and a quality control assay for measuring the integrity of epithelial monolayers cultured under static conditions in vitro, however, there is no standard methodology for its application to microfluidic organ-on-a-chip (organ chip) cultures. Here, we describe a new microfluidic organ chip design that contains embedded electrodes, and we demonstrate its utility for assessing formation and disruption of barrier function both within a human lung airway chip lined by a fully differentiated mucociliary human airway epithelium and in a human gut chip lined by intestinal epithelial cells...
June 9, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28488235/emulating-host-microbiome-ecosystem-of-human-gastrointestinal-tract-in-vitro
#2
Gun-Seok Park, Min Hee Park, Woojung Shin, Connie Zhao, Sameer Sheikh, So Jung Oh, Hyun Jung Kim
The human gut microbiome performs prodigious physiological functions such as production of microbial metabolites, modulation of nutrient digestion and drug metabolism, control of immune system, and prevention of infection. Paradoxically, gut microbiome can also negatively orchestrate the host responses in diseases or chronic disorders, suggesting that the regulated and balanced host-gut microbiome crosstalk is a salient prerequisite in gastrointestinal physiology. To understand the pathophysiological role of host-microbiome crosstalk, it is critical to recreate in vivo relevant models of the host-gut microbiome ecosystem in human...
May 10, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28468904/host-genome-influence-on-gut-microbial-composition-and-microbial-prediction-of-complex-traits-in-pigs
#3
Amelia Camarinha-Silva, Maria Maushammer, Robin Wellmann, Marius Vital, Siegfried Preuss, Jörn Bennewitz
The aim of the present study was to analyze the interplay between gastrointestinal tract (GIT) microbiota, host genetics and complex traits in pigs using extended quantitative-genetic methods. The study design consisted of 207 pigs that were housed and slaughtered under standardized conditions and phenotyped for daily gain, feed intake and feed conversion rate. The pigs were genotyped with a standard 60K SNP chip. The GIT microbiota composition was analyzed by 16S rRNA gene amplicon sequencing technology. Eight from 49 investigated bacteria genera showed a significant narrow sense host heritability, ranging from 0...
May 3, 2017: Genetics
https://www.readbyqxmd.com/read/28451924/microfluidic-gut-on-a-chip-with-three-dimensional-villi-structure
#4
Kyu-Young Shim, Dongwook Lee, Jeonghun Han, Nam-Trung Nguyen, Sungsu Park, Jong Hwan Sung
Current in vitro gut models lack physiological relevance, and various approaches have been taken to improve current cell culture models. For example, mimicking the three-dimensional (3D) tissue structure or fluidic environment has been shown to improve the physiological function of gut cells. Here, we incorporated a collagen scaffold that mimics the human intestinal villi into a microfluidic device, thus providing cells with both 3D tissue structure and fluidic shear. We hypothesized that the combined effect of 3D structure and fluidic shear may provide cells with adequate stimulus to induce further differentiation and improve physiological relevance...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28146569/human-gut-on-a-chip-supports-polarized-infection-of-coxsackie-b1-virus-in-vitro
#5
Remi Villenave, Samantha Q Wales, Tiama Hamkins-Indik, Efstathia Papafragkou, James C Weaver, Thomas C Ferrante, Anthony Bahinski, Christopher A Elkins, Michael Kulka, Donald E Ingber
Analysis of enterovirus infection is difficult in animals because they express different virus receptors than humans, and static cell culture systems do not reproduce the physical complexity of the human intestinal epithelium. Here, using coxsackievirus B1 (CVB1) as a prototype enterovirus strain, we demonstrate that human enterovirus infection, replication and infectious virus production can be analyzed in vitro in a human Gut-on-a-Chip microfluidic device that supports culture of highly differentiated human villus intestinal epithelium under conditions of fluid flow and peristalsis-like motions...
2017: PloS One
https://www.readbyqxmd.com/read/28113162/omics-tech-gut-on-a-chip-and-bacterial-engineering-new-approaches-for-treating-inflammatory-bowel-diseases
#6
Leslie Mertz
It was six years ago that fecal transplantation first received prominent media attention and the public began to fully appreciate that the bacteria and other microbes in their bodies could have a real impact on health.
September 2016: IEEE Pulse
https://www.readbyqxmd.com/read/28074384/microfluidic-gut-liver-chip-for-reproducing-the-first-pass-metabolism
#7
Aerim Choe, Sang Keun Ha, Inwook Choi, Nakwon Choi, Jong Hwan Sung
After oral intake of drugs, drugs go through the first pass metabolism in the gut and the liver, which greatly affects the final outcome of the drugs' efficacy and side effects. The first pass metabolism is a complex process involving the gut and the liver tissue, with transport and reaction occurring simultaneously at various locations, which makes it difficult to be reproduced in vitro with conventional cell culture systems. In an effort to tackle this challenge, here we have developed a microfluidic gut-liver chip that can reproduce the dynamics of the first pass metabolism...
March 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28062270/the-role-of-gut-microbiota-in-health-and-disease-in%C3%A2-vitro-modeling-of-host-microbe-interactions-at-the-aerobe-anaerobe-interphase-of-the-human-gut
#8
REVIEW
Julius Z H von Martels, Mehdi Sadaghian Sadabad, Arno R Bourgonje, Tjasso Blokzijl, Gerard Dijkstra, Klaas Nico Faber, Hermie J M Harmsen
The microbiota of the gut has many crucial functions in human health. Dysbiosis of the microbiota has been correlated to a large and still increasing number of diseases. Recent studies have mostly focused on analyzing the associations between disease and an aberrant microbiota composition. Functional studies using (in vitro) gut models are required to investigate the precise interactions that occur between specific bacteria (or bacterial mixtures) and gut epithelial cells. As most gut bacteria are obligate or facultative anaerobes, studying their effect on oxygen-requiring human gut epithelial cells is technically challenging...
April 2017: Anaerobe
https://www.readbyqxmd.com/read/27743363/analysis-of-milk-oligosaccharides-by-mass-spectrometry
#9
Lauren D Wu, L Renee Ruhaak, Carlito B Lebrilla
Human milk oligosaccharides (HMOs) are a highly abundant constituent in human milk, and its protective and prebiotic properties have attracted considerable attention. HMOs have been shown to directly and indirectly benefit the overall health of the infant due to a number of functions including serving as a beneficial food for gut bacteria, block to pathogens, and aiding in brain development. Researchers are currently exploring whether these structures may act as possible disease and nutrition biomarkers. Because of this, rapid-throughput methods are desired to investigate biological activity in large patient sets...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27684630/co-culture-of-living-microbiome-with-microengineered-human-intestinal-villi-in-a-gut-on-a-chip-microfluidic-device
#10
Hyun Jung Kim, Jaewon Lee, Jin-Ha Choi, Anthony Bahinski, Donald E Ingber
Here, we describe a protocol to perform long-term co-culture of multi-species human gut microbiome with microengineered intestinal villi in a human gut-on-a-chip microphysiological device. We recapitulate the intestinal lumen-capillary tissue interface in a microfluidic device, where physiological mechanical deformations and fluid shear flow are constantly applied to mimic peristalsis. In the lumen microchannel, human intestinal epithelial Caco-2 cells are cultured to form a 'germ-free' villus epithelium and regenerate small intestinal villi...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27522435/characterization-of-porcine-milk-oligosaccharides-during-early-lactation-and-their-relation-to-the-fecal-microbiome
#11
COMPARATIVE STUDY
J Salcedo, S A Frese, D A Mills, D Barile
The composition of porcine milk oligosaccharides (PMO) was analyzed during early lactation and their relation to piglet gut microbiome was investigated. Pigs are considered ideal intestinal models to simulate humans because of the striking similarity in intestinal physiopathology to humans. The evolution of PMO was investigated in the milk from 3 healthy sows at prefarrowing, farrowing, and d 7 and 14 postpartum by Nano-LC Chip Quadrupole-Time-of-Flight mass spectrometer (Agilent Technologies, Santa Clara, CA)...
October 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27291426/human-gut-on-a-chip-technology-will-this-revolutionize-our-understanding-of-ibd-and-future-treatments
#12
EDITORIAL
Jaewon Lee, Jin-Ha Choi, Hyun Jung Kim
No abstract text is available yet for this article.
August 2016: Expert Review of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/27271944/gut-content-analysis-of-a-phloem-feeding-insect-bactericera-cockerelli-hemiptera-triozidae
#13
W Rodney Cooper, David R Horton, Thomas R Unruh, Stephen F Garczynski
Potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae), is a key pest of potato (Solanum tuberosum L., Solanales: Solanaceae) and a vector of "Candidatus Liberibacter solanacearum," the pathogen associated with zebra chip disease. In addition to its presence on cultivated crops, the psyllid regularly occurs on numerous uncultivated annual and perennial species within the Solanaceae. A better understanding of landscape-level ecology of B. cockerelli would substantially improve our ability to predict which potato fields are most likely to be colonized by infected psyllids...
August 2016: Environmental Entomology
https://www.readbyqxmd.com/read/27231242/effects-of-dietary-fibre-source-on-microbiota-composition-in-the-large-intestine-of-suckling-piglets
#14
Lingli Zhang, Chunlong Mu, Xiangyu He, Yong Su, Shengyong Mao, Jing Zhang, Hauke Smidt, Weiyun Zhu
This study aimed to investigate the effects of dietary fibre sources on the gut microbiota in suckling piglets, and to test the hypothesis that a moderate increase of dietary fibre may affect the gut microbiota during the suckling period. Suckling piglets were fed different fibre-containing diets or a control diet from postnatal day 7 to 22. Digesta samples from cecum, proximal colon and distal colon were used for Pig Intestinal Tract Chip analysis. The data showed that the effects of fibre-containing diet on the gut microbiota differed in the fibre source and gut location...
July 2016: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/27147463/the-third-dimension-new-developments-in-cell-culture-models-for-colorectal-research
#15
REVIEW
Joana F S Pereira, Nikhil T Awatade, Cláudia A Loureiro, Paulo Matos, Margarida D Amaral, Peter Jordan
Cellular models are important tools in various research areas related to colorectal biology and associated diseases. Herein, we review the most widely used cell lines and the different techniques to grow them, either as cell monolayer, polarized two-dimensional epithelia on membrane filters, or as three-dimensional spheres in scaffold-free or matrix-supported culture conditions. Moreover, recent developments, such as gut-on-chip devices or the ex vivo growth of biopsy-derived organoids, are also discussed. We provide an overview on the potential applications but also on the limitations for each of these techniques, while evaluating their contribution to provide more reliable cellular models for research, diagnostic testing, or pharmacological validation related to colon physiology and pathophysiology...
November 2016: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/27026084/effect-of-a-long-term-high-protein-diet-on-survival-obesity-development-and-gut-microbiota-in-mice
#16
COMPARATIVE STUDY
Pia Kiilerich, Lene Secher Myrmel, Even Fjære, Qin Hao, Floor Hugenholtz, Si Brask Sonne, Muriel Derrien, Lone Møller Pedersen, Rasmus Koefoed Petersen, Alicja Mortensen, Tine Rask Licht, Maria Unni Rømer, Ulla Birgitte Vogel, Linn Jeanette Waagbø, Natasa Giallourou, Qiang Feng, Liang Xiao, Chuan Liu, Bjørn Liaset, Michiel Kleerebezem, Jun Wang, Lise Madsen, Karsten Kristiansen
Female C57BL/6J mice were fed a regular low-fat diet or high-fat diets combined with either high or low protein-to-sucrose ratios during their entire lifespan to examine the long-term effects on obesity development, gut microbiota, and survival. Intake of a high-fat diet with a low protein/sucrose ratio precipitated obesity and reduced survival relative to mice fed a low-fat diet. By contrast, intake of a high-fat diet with a high protein/sucrose ratio attenuated lifelong weight gain and adipose tissue expansion, and survival was not significantly altered relative to low-fat-fed mice...
June 1, 2016: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/26960338/foxo3b-but-not-foxo3a-activates-cyp19a1a-in-epinephelus-coioides
#17
Qiongyou Liu, Yang Zhang, Boyang Shi, Huijie Lu, Lihong Zhang, Weimin Zhang
FOXO3 has been shown to be a critical transcription factor for folliculogenesis in mammals, while the information on its roles in reproduction of nonmammalian vertebrates remains scarce. In this study, two foxo3 homologs, namely foxo3a and foxo3b, were identified in a teleost, the orange-spotted grouper Epinephelus coioides. foxo3a was mainly expressed in the central nervous system, ovary, and gut whereas foxo3b was expressed ubiquitously in tissues examined. In contrast to the dominant expression of mammalian FOXO3 in germ cells but barely detectable in ovarian follicular cells, immunoreactive Foxo3a and Foxo3b were identified both in the ovarian germ cells and follicular cells...
May 2016: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/26888480/a-reductionist-metastasis-on-a-chip-platform-for-in-vitro-tumor-progression-modeling-and-drug-screening
#18
Aleksander Skardal, Mahesh Devarasetty, Steven Forsythe, Anthony Atala, Shay Soker
Current animal and 2-D cell culture models employed in metastasis research and drug discovery remain poor mimics of human cancer physiology. Here we describe a "metastasis-on-a-chip" system allowing real time tracking of fluorescent colon cancer cells migrating from hydrogel-fabricated gut constructs to downstream liver constructs within a circulatory fluidic device system that responds to environmental manipulation and drug treatment. Devices consist of two chambers in which gut and liver constructs are housed independently, but are connected in series via circulating fluid flow...
September 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/26668389/contributions-of-microbiome-and-mechanical-deformation-to-intestinal-bacterial-overgrowth-and-inflammation-in-a-human-gut-on-a-chip
#19
Hyun Jung Kim, Hu Li, James J Collins, Donald E Ingber
A human gut-on-a-chip microdevice was used to coculture multiple commensal microbes in contact with living human intestinal epithelial cells for more than a week in vitro and to analyze how gut microbiome, inflammatory cells, and peristalsis-associated mechanical deformations independently contribute to intestinal bacterial overgrowth and inflammation. This in vitro model replicated results from past animal and human studies, including demonstration that probiotic and antibiotic therapies can suppress villus injury induced by pathogenic bacteria...
January 5, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/26551449/localization-of-candidatus-liberibacter-solanacearum-and-evidence-for-surface-appendages-in-the-potato-psyllid-vector
#20
J M Cicero, T W Fisher, J K Brown
The potato psyllid Bactericera cockerelli is implicated as the vector of the causal agent of zebra chip of potato and vein-greening of tomato diseases. Until now, visual identification of bacteria in the genus 'Candidatus Liberibacter' has relied on direct imaging by light and electron microscopy without labeling, or with whole-organ fluorescence labeling only. In this study, aldehyde fixative followed by a coagulant fixative, was used to process adult psyllids for transmission electron microscopy (TEM) colloidal gold in situ hybridization experiments...
February 2016: Phytopathology
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