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Microbiology specimen collection

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https://www.readbyqxmd.com/read/29685676/effectiveness-of-a-novel-specimen-collection-system-in-reducing-blood-culture-contamination-rates
#1
Mary Bell, Catherine Bogar, Jessica Plante, Kristen Rasmussen, Sharon Winters
PROBLEM: False-positive blood-culture results due to skin contamination of samples remain a persistent problem for health care providers. Our health system recognized that our rates of contamination across the 4 emergency department campuses were above the national average. METHODS: A unique specimen collection system was implemented throughout the 4 emergency departments and became the mandatory way to collect adult blood cultures. The microbiology laboratory reported contamination rates weekly to manage potential problems; 7 months of data are presented here...
April 20, 2018: Journal of Emergency Nursing: JEN: Official Publication of the Emergency Department Nurses Association
https://www.readbyqxmd.com/read/29657531/antimicrobial-efficacy-of-2-5-sodium-hypochlorite-2-chlorhexidine-and-ozonated-water-as-irrigants-in-mesiobuccal-root-canals-with-severe-curvature-of-mandibular-molars
#2
Sergio Luiz Pinheiro, Caio Cesar da Silva, Lucas Augusto da Silva, Marina P Cicotti, Carlos Eduardo da Silveira Bueno, Carlos Eduardo Fontana, Letícia R Pagrion, Natália P Dalmora, Thaís T Daque, Francisco Uf de Campos
Objective: The aim of this study is to evaluate the antimicrobial efficacy of 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water on biofilms of Enterococcus faecalis , Streptococcus mutans , and Candida albicans in mesiobuccal root canals with severe curvature of mandibular molars. Materials and Methods: This was an experimental ex vivo study in microbiologic laboratory. Sixty mesiobuccal root canals with severe curvature of mandibular molars were contaminated with standard strains of E...
January 2018: European Journal of Dentistry
https://www.readbyqxmd.com/read/29644884/prospective-analysis-of-surgical-bone-margins-after-partial-foot-amputation-in-diabetic-patients-admitted-with-moderate-to-severe-foot-infections
#3
Brian M Schmidt, Jonathan B McHugh, Rajiv M Patel, James S Wrobel
BACKGROUND: Osteomyelitis is common in diabetic foot infections and medical management can lead to poor outcomes. Surgical management involves sending histopathologic and microbiologic specimens which guides future intervention. We examined the effect of obtainment of surgical margins in patients undergoing forefoot amputations to identify patient characteristics associated with outcomes. Secondary aims included evaluating interobserver reliability of histopathologic data at both the distal-to and proximal-to surgical bone margin...
April 1, 2018: Foot & Ankle Specialist
https://www.readbyqxmd.com/read/29617982/gepotidacin-for-the-treatment-of-uncomplicated-urogenital-gonorrhea-a-phase-2-randomized-dose-ranging-single-oral-dose-evaluation
#4
Stephanie N Taylor, David H Morris, Ann K Avery, Kimberly A Workowski, Byron E Batteiger, Courtney A Tiffany, Caroline R Perry, Aparna Raychaudhuri, Nicole E Scangarella-Oman, Mohammad Hossain, Etienne F Dumont
Background: In this phase 2 study, we evaluated the efficacy and safety of oral gepotidacin, a novel triazaacenaphthylene bacterial type II topoisomerase inhibitor, for the treatment of uncomplicated urogenital gonorrhea. Methods: Adult participants with suspected urogenital gonorrhea were enrolled and completed baseline (day 1) and test-of-cure (days 4-8) visits. Pretreatment and posttreatment urogenital swabs were collected for Neisseria gonorrhoeae (NG) culture and susceptibility testing...
April 2, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29615167/pseudomonas-aeruginosa-evaluation-of-pathogen-burden-and-drug-resistance-trends-in-a-tertiary-care-hospital
#5
Muhammad Saeed, Farhan Rasheed, Raja Kamran Afzal, Shahida Hussain, Saba Riaz, Adeel Ahmad
OBJECTIVE: To evaluate the pathogen burden and antibiotic-resistance trends of Pseudomonas aeruginosa among hospitalised patients at a tertiary care hospital. STUDY DESIGN: Retrospective, hospital record-based, cross-sectional study. PLACE AND DURATION OF STUDY: Microbiology Laboratory, Allama Iqbal Medical College/Jinnah Hospital, Lahore, from January 2014 to December 2016. METHODOLOGY: A total of 5,960 samples were collected from clinically suspected cases of bacterial infections, admitted to the hospital...
April 2018: Journal of the College of Physicians and Surgeons—Pakistan: JCPSP
https://www.readbyqxmd.com/read/29578101/diagnostic-accuracy-of-multiplex-real-time-pcr-approaches-in-compared-with-cultivation-based-detection-methods-monitoring-the-endopathogenic-microbiota-pre-and-post-photo-activated-disinfection
#6
Maryam Pourhajibagher, Abbas Bahador
BACKGROUND: Several microbial species that have been implicated in the pathogenesis of endodontic diseases colonize the infected root canal system. Since the complete removal of endopathogenic agents is essential in endodontic infections therapy, photo-activated disinfection (PAD) is suggested as an alternative method to traditional antimicrobial therapy. Recent studies reported that the molecular methods with low sensitivity and high efficiency to identify fastidious anaerobic endopathogenic microbiota can be replaced by the cultivation-based approaches...
March 22, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29569277/use-of-genetic-climatic-and-microbiological-data-to-inform-reintroduction-of-a-regionally-extinct-butterfly
#7
Vlad Dincă, Zsolt Bálint, Raluca Vodă, Leonardo Dapporto, Paul D N Hebert, Roger Vila
Species reintroductions are increasingly used as means of mitigating biodiversity loss. Besides habitat quality at the site targeted for reintroduction, the choice of source population can be critical for success. The butterfly Melanargia russiae (Esper's marbled white) was extirpated from Hungary over 100 years ago, and a reintroduction program has recently been approved. We used museum specimens of this butterfly, mitochondrial DNA data (mtDNA), endosymbiont screening, and climatic-similarity analyses to determine which extant populations should be used for its reintroduction...
March 22, 2018: Conservation Biology: the Journal of the Society for Conservation Biology
https://www.readbyqxmd.com/read/29517630/impact-of-antibiotic-resistant-bacterial-and-fungal-infections-in-outcome-of-acute-pancreatitis
#8
Praneeth Moka, Pooja Goswami, Arti Kapil, Immaculata Xess, Vishnubhatla Sreenivas, Anoop Saraya
OBJECTIVES: The knowledge about pathogens and their antibiotic susceptibility patterns is essential to select an appropriate antibiotic. METHODS: We investigated the microbiological profile in pancreatic and extrapancreatic infections, and antibiotic sensitivity pattern in patients with acute pancreatitis. RESULTS: Of 556 patients with acute pancreatitis, only 189 developed bacterial infection; however, bacteremia was present in 42 patients (7...
April 2018: Pancreas
https://www.readbyqxmd.com/read/29511571/private-collection-high-correlation-of-sample-collection-and-patient-admission-date-in-clinical-microbiological-testing-complicates-sharing-of-phylodynamic-metadata
#9
Ryan C Shean, Alexander L Greninger
Infectious pathogens are known for their rapid evolutionary rates with new mutations arising over days to weeks. The ability to rapidly recover whole genome sequences and analyze the spread and evolution of pathogens using genetic information and pathogen collection dates has lead to interest in real-time tracking of infectious transmission and outbreaks. However, the level of temporal resolution afforded by these analyses may conflict with definitions of what constitutes protected health information (PHI) and privacy requirements for de-identification for publication and public sharing of research data and metadata...
January 2018: Virus Evolution
https://www.readbyqxmd.com/read/29506629/the-prevalence-of-cmy-2-oxa-48-and-kpc-2-genes-in-clinical-isolates-of-klebsiella-spp
#10
Talayeh Tavakoly, Samar Jamali, Ali Mojtahedi, Mohammadali Khan Mirzaei, Mohammad Shenagari
Klebsiella pneumoniae is a Gram-negative bacterium which causes several human infections. Treatment of infections related to K. pneumoniae has become problematic, because of increasing trend of extended spectrum β-lactamases producing (ESBLs) strains. The present study was aimed to detect the prevalence of ESBL-producing Klebsiella spp. and KPC-2, CMY-2 and OXA-48 β-lactamase encoding genes in clinical isolates of Klebsiella spp. isolated from hospitalized patients. In this cross-sectional study carried out from February to August 2014, 144 isolates of Klebsiella spp...
February 28, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/29501453/staphylococcus-aureus-in-the-airways-of-cystic-fibrosis-patients-a-retrospective-long-term-study
#11
Mathias Schwerdt, Claudia Neumann, Bianca Schwartbeck, Stefanie Kampmeier, Susann Herzog, Dennis Görlich, Angelika Dübbers, Jörg Große-Onnebrink, Christina Kessler, Peter Küster, Holger Schültingkemper, Janina Treffon, Georg Peters, Barbara C Kahl
BACKGROUND: Cystic fibrosis (CF) is an autosomal recessive disease associated with chronic airway infections by Staphylococcus aureus as one of the earliest and most prevalent pathogens. We conducted a retrospective study to determine the S. aureus infection status of CF patients treated since 1994 at two certified CF-centres in Münster, Germany, to get insights into the dynamics of S. aureus airway infection and the clinical impact on lung function on a long-term perspective. MATERIALS AND METHODS: We used data from our microbiological database collected between 1994 and 2016 for patients treated at two centres in Münster, Germany, respectively, to determine the infection status for S...
February 24, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29489887/accelerating-access-to-quality-tb-care-for-pediatric-tb-cases-through-better-diagnostic-strategy-in-four-major-cities-of-india
#12
Neeraj Raizada, Sunil D Khaparde, Virender Singh Salhotra, Raghuram Rao, Aakshi Kalra, Soumya Swaminathan, Ashwani Khanna, Kamal Kishore Chopra, M Hanif, Varinder Singh, K R Umadevi, Sreenivas Achuthan Nair, Sophie Huddart, C H Surya Prakash, Shalini Mall, Pooja Singh, B K Saha, Claudia M Denkinger, Catharina Boehme, Sanjay Sarin
BACKGROUND: Diagnosis of TB in children is challenging, and is largely based on positive history of contact with a TB case, clinical and radiological findings, often without microbiological confirmation. Diagnostic efforts are also undermined by challenges in specimen collection and the limited availability of high sensitivity, rapid diagnostic tests that can be applied with a quick turnaround time. The current project was undertaken in four major cities of India to address TB diagnostic challenges in pediatric population, by offering free of cost Xpert testing to pediatric presumptive TB cases, thereby paving the way for better TB care...
2018: PloS One
https://www.readbyqxmd.com/read/29486354/an-in-vivo-evaluation-of-microbial-diversity-before-and-after-the-photo-activated-disinfection-in-primary-endodontic-infections-traditional-phenotypic-and-molecular-approaches
#13
Maryam Pourhajibagher, Abbas Bahador
BACKGROUND: It is essential to identify the root canal microbial diversity and count due to the polymicrobial nature of the primary endodontic infection that is associated with the microbial diversity and increased resistance to the antimicrobial agents. Photo-activated disinfection (PAD), also known as antimicrobial photodynamic therapy, is a new promising non-antibiotic approach, studied to prevent microbial resistance and treatment failure. MATERIALS AND METHODS: In this study, we investigated the effect of PAD on reduction of microbial diversity and count, related with primary endodontic infections...
February 24, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29479554/molecular-identification-of-bacteria-in-intra-abdominal-abscesses-using-deep-sequencing
#14
Andrew Kozlov, Lorenzo Bean, Emilie V Hill, Lisa Zhao, Eric Li, Gary P Wang
Background: Intra-abdominal abscesses are localized collections of pus, which generally arise from a breach in the normal mucosal defense barrier that allows bacteria from gastrointestinal tract, and less commonly from the gynecologic or urinary tract, to induce inflammation, resulting in an infection. The microbiology of these abscesses is usually polymicrobial, associated with the primary disease process. However, the microbial identity, diversity and richness in intra-abdominal abscesses have not been well characterized, due in part to the difficulty in cultivating commensal organisms using standard culture-based techniques...
February 2018: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/29479244/physician-perspectives-on-vaccination-and-diagnostic-testing-in-children-with-gastroenteritis-a-primary-care-physician-survey
#15
Arissa J Sperou, James A Dickinson, Bonita Lee, Marie Louie, Xiao-Li Pang, Linda Chui, Otto G Vanderkooi, Stephen B Freedman
Objectives: Gastroenteritis remains a common paediatric illness. Little is known about physician knowledge of enteric pathogen diagnostic tests. At the time of study conduct, Alberta lacked a publicly funded rotavirus vaccination program and knowledge of primary care physician perspectives was lacking. We sought to ascertain diagnostic testing methods and to understand knowledge and perceptions regarding enteric pathogen vaccination. Methods: A 30-item electronic survey was distributed across Alberta's five health care zones...
September 2017: Paediatrics & Child Health
https://www.readbyqxmd.com/read/29467997/investigation-of-class-i-ii-and-iii-integrons-among-acinetobacter-baumannii-isolates-from-hospitalized-patients-in-isfahan-iran
#16
Mehrdad Halaji, Aliakbar Rezaei, Mehrdad Zalipoor, Jamshid Faghri
Objectives: This study aimed to determine the prevalence of class I, II, and III integrons among clinical Acinetobacter baumannii isolates collected from hospitalized patients. Methods: This cross-sectional study was conducted at two teaching hospitals in Isfahan, Iran, from October 2015 to October 2016. A total of 147 non-duplicate A. baumannii isolates were collected from clinical specimens and identified as A. baumannii using standard microbiological methods and confirmed by genotyping...
January 2018: Oman Medical Journal
https://www.readbyqxmd.com/read/29458156/how-to-isolate-identify-and-determine-antimicrobial-susceptibility-of-anaerobic-bacteria-in-routine-laboratories
#17
REVIEW
Elisabeth Nagy, Lyudmila Boyanova, Ulrik S Justesen
BACKGROUND: There has been an increased interest in the study of anaerobic bacteria, which cause human infection during the past decade. Many new genera and species have been described by to use 16S rRNA gene sequencing of clinical isolates obtained from different infection sites with commercially available special culture media to support the growth of anaerobes. Several systems, such as anaerobic pouches, boxes, jars and chambers provide suitable anaerobic culture conditions to isolate even strict anaerobic bacteria successfully from clinical specimens...
February 16, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29454845/evaluation-of-a-new-multiplex-pcr-assay-paragenie-g-amoeba-real-time-pcr-kit-targeting-giardia-intestinalis-entamoeba-histolytica-and-entamoeba-dispar-entamoeba-moshkovskii-from-stool-specimens-evidence-for-the-limited-performances-of-microscopy-based-approach
#18
Florent Morio, Stéphane Valot, Adrien Laude, Guillaume Desoubeaux, Nicolas Argy, Céline Nourrisson, Christelle Pomares, Marie Machouart, Yohann Le Govic, Frédéric Dalle, Françoise Botterel, Nathalie Bourgeois, Estelle Cateau, Marion Leterrier, Fakhri Jeddi, Manuel Gaboyard, Patrice Le Pape
OBJECTIVES: Besides the potential to identify a wide variety of gastrointestinal parasites, microscopy remains the gold standard in clinical microbiology for amoeba species identification and especially, when coupled with adhesin detection, to discriminate the pathogenic E. histolytica from its sisters but non-pathogenic species E. dispar/E. moschovskii. However, this approach is time-consuming, requires a high-level of expertise that can be jeopardized considering the low prevalence of gastrointestinal parasites in non-endemic countries...
February 15, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29444832/differential-contributions-of-specimen-types-culturing-and-16s-rrna-sequencing-in-diagnosis-of-prosthetic-joint-infections
#19
Lone Heimann Larsen, Vesal Khalid, Yijuan Xu, Trine Rolighed Thomsen, Henrik C Schønheyder
Prosthetic joint failure is mainly caused by infection, aseptic failure (AF), and mechanical problems. Infection detection has been improved with modified culture methods and molecular diagnostics. However, comparisons between modified and conventional microbiology methods are difficult, due to variations in specimen sampling. In this prospective, multidisciplinary study of hip or knee prosthetic failures, we assessed the contributions of different specimen types, extended culture incubations, and 16S rRNA sequencing for diagnosing prosthetic joint infections (PJI)...
February 14, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29427686/laboratory-diagnosis-of-urinary-tract-infections-towards-a-bilulu-consensus-guideline
#20
REVIEW
Matthijs Oyaert, Britt Van Meensel, Reinoud Cartuyvels, Johan Frans, Wim Laffut, Patricia Vandecandelaere, Hans De Beenhouwer
Urinary tract infections (UTI) are very common throughout life and account for the majority of the workload in the clinical microbiology laboratory. Clear instructions for the interpretation of urine cultures by the laboratory technicians are indispensable to obtain standardized, reliable, and clinically useful results. In literature, there is often a lack of evidence-based practice in processing urinary samples in the laboratory. In this consensus document, the BILULU Study Group presents a practical approach for the implementation of existing guidelines for the culture of urine in the microbiology laboratory and offers answers for issues where no clear solution is available in the guidelines...
March 2018: Journal of Microbiological Methods
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