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Microbiology specimen collection

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https://www.readbyqxmd.com/read/29225753/study-of-bacterial-flora-associated-with-mobile-phones-of-healthcare-workers-and-non-healthcare-workers
#1
Raghavendra Rao Morubagal, Sowmya Govindanahalli Shivappa, Rashmi Padmanabha Mahale, Sumana Mhadevaiah Neelambike
Background and Objectives: Despite improvements in modern diagnosis and therapies, hospital acquired infections remain a leading problem of global health systems. Healthcare workers mobile phones is a reservoir for potential pathogens. Despite the high possibility of being contaminated, mobile phones are rarely clean and are often touched during or after examination of patients and handling of specimens without proper hand washing. The main objective of the present study was to isolate, identify different types of bacteria and their antibiotic sensitivity from mobile phones of healthcare workers and non-health-care workers...
June 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29221301/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-a-powerful-tool-for-identification-of-corynebacterium-species
#2
Rong Bao, Xiaodong Gao, Bijie Hu, Zhaoyan Zhou
Background: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a powerful tool that has initiated a revolution in the clinical microbiology laboratory for identification of nosocomial pathogens. The efficacy of MALDI-TOF MS produced by bioMerieux, Marcy l'Etoile, France (VITEK MS) for detecting Corynebacterium remains unknown. Methods: Corynebacterium isolates were isolated from clinical specimen in a tertial teaching hospital from 2012 to 2013...
September 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29217418/molecular-characterization-of-virulence-and-antimicrobial-resistance-profile-of-shigella-species-isolated-from-children-with-moderate-to-severe-diarrhea-in-northeastern-brazil
#3
Pedro Henrique Quintela Soares Medeiros, Aldo Ângelo Moreira Lima, Marjorie Moreira Guedes, Alexandre Havt, Mariana Duarte Bona, Luís Carlos Rey, Alberto Melo Soares, Richard Littleton Guerrant, Bernhard H Weigl, Ila Fernanda Nunes Lima
Molecular characterization of virulence and antimicrobial resistance profiles were determined for Shigella species isolated from children with diarrhea in Fortaleza, Brazil. Fecal specimens were collected along with socioeconomic and clinical data from children with moderate to severe diarrhea requiring emergency care. Shigella spp. were isolated by standard microbiological techniques, and we developed 4 multiplex polymerase chain reaction assays to detect 16 virulence-related genes (VRGs). Antimicrobial susceptibility tests were performed using disk diffusion assays...
November 10, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29208879/oncoe6-positivity-among-via-positive-suspected-cases-from-colposcopy-clinic-of-mymensingh-medical-college-hospital-mymensingh
#4
F Nahar, M A Hossain, S K Paul, M U Ahmed, S Khatun, N Akhter, G R Bhuiyan, S A Nasreen, S Roy, T K Barman, N Laskar, H Begum, S Abedin, N Haque, S Ahmed, N Kobayashi
Cervical cancer is a major world health problem for women. It is the fourth most leading cause of death in women around the world. High risk HPV DNA has been shown to be present in 99.7% of cervical cancers worldwide. Oncoprotein E6 and E7 play an important role in the development of cervical cancer which can be detected by OncoE6 cervical test. This Cross sectional observational study was performed to detect E6 Oncoprotein from cervical swab by OncoE6 cervical test. Following universal safety precautions a total of 47 endocervical swabs were collected from Colposcopy clinic of Obstetrics and Gynaecology department of Mymensingh Medical College Hospital (MMCH), Mymensingh, Bangladesh from January 2015 to December 2015...
October 2017: Mymensingh Medical Journal: MMJ
https://www.readbyqxmd.com/read/29207709/isolation-of-the-rare-opportunistic-yeast-saprochaete-capitata-from-clinical-samples-experience-from-a-tertiary-care-hospital-in-southern-india-and-a-brief-review-of-the-literature
#5
Umabala Pamidimukkala, Anuradha Kancharla, Sukanya Sudhaharan, Sadashivudu Gundeti, Surendra Mandarapu, Vamsi Krishna Nagalla, Sree Bhushan Raju, Sandhya Devi Karanam
Introduction: Saprochaete capitata (Teleomorph: Magnusiomyces capitatus) is a ubiquitous yeast found in environmental sources such as soil, water, air, plants and dairy products. It is also a part of the normal microbial flora in humans. The yeast is being increasingly reported as an opportunistic pathogen, especially in patients in the haemato-oncology setting, the infection being often mistakenly diagnosed as invasive candidiasis. Aim: To review the epidemiological, clinical and microbiological features of six patients admitted in our hospital over a period of 10 years (from January 2007 to December 2016), from whom Saprochaete capitata was isolated...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29204224/bacteriology-and-antibiogram-of-urinary-tract-infection-among-female-patients-in-a-tertiary-health-facility-in-south-eastern-nigeria
#6
REVIEW
Angus N Oli, Vivian B Akabueze, Chijioke E Ezeudu, George U Eleje, Obiora S Ejiofor, Ifeanyichukwu U Ezebialu, Charlotte B Oguejiofor, Ifeoma M Ekejindu, George O Emechebe, Kenneth N Okeke
Background: Urinary Tract Infection (UTI) is a common contagion among men and women with the incidence relatively higher among women due to their differing anatomy. An understanding of the kind of pathogens implicated in urinary tract infections as well as antibiotic susceptibility profiling may help the clinician make rationally correct empirical choice in their treatment. Objective: This study is aimed at determining the type and antibiotic susceptibility pattern of bacterial uropathogens isolated from female patients attending Chukwuemeka Odumegwu Ojukwu University Teaching Hospital (COOUTH), Awka, Nigeria...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/29166867/clinical-and-virological-factors-associated-with-gastrointestinal-symptoms-in-patients-with-acute-respiratory-infection-a-two-year-prospective-study-in-general-practice-medicine
#7
Laetitia Minodier, Shirley Masse, Lisandru Capai, Thierry Blanchon, Pierre-Emmanuel Ceccaldi, Sylvie van der Werf, Thomas Hanslik, Remi Charrel, Alessandra Falchi
BACKGROUND: Gastrointestinal (GI) symptoms, such as diarrhea, vomiting, abdominal pain and nausea are not an uncommon manifestation of an acute respiratory infection (ARI). We therefore evaluated clinical and microbiological factors associated with the presence of GI symptoms in patients consulting a general practitioner (GP) for ARI. METHODS: Nasopharyngeal swabs, stool specimens and clinical data from patients presenting to GPs with an ARI were prospectively collected during two winter seasons (2014-2016)...
November 22, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29144593/risk-factors-for-seasonal-influenza-virus-detection-in-stools-of-patients-consulting-in-general-practice-for-acute-respiratory-infections-in-france-2014-2016
#8
L Minodier, S Masse, L Capai, T Blanchon, P E Ceccaldi, S van der Werf, T Hanslik, N R Charrel, A Falchi
BACKGROUND: Previous studies reported detection of influenza RNA in stools of patients with seasonal influenza infection. While this detection may have a clinical significance, other factors may influence the stools positivity for influenza viruses. OBJECTIVES: The objective of this study was to investigate demographical, clinical and microbiological factors which could favour the presence of influenza viral RNA in the stools of patients with laboratory-confirmed influenza infection...
November 16, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/29119726/cotrimoxazole-prophylaxis-was-associated-with-enteric-commensal-bacterial-resistance-among-hiv-exposed-infants-in-a-randomized-controlled-trial-botswana
#9
Kathleen M Powis, Sajini Souda, Shahin Lockman, Gbolahan Ajibola, Kara Bennett, Jean Leidner, Michael D Hughes, Sikhulile Moyo, Erik van Widenfelt, Haruna B Jibril, Joseph Makhema, Max Essex, Roger L Shapiro
INTRODUCTION: Despite declining risk of vertical HIV transmission, prophylactic cotrimoxazole (CTX) remains widely used to reduce morbidity and mortality in the event of HIV infection among exposed infants, with an inherent risk of conferring commensal antimicrobial resistance. Using data from a randomized, placebo-controlled trial of infant CTX prophylaxis, we sought to quantify emergence of antibiotic resistance. METHODS: HIV-exposed uninfected infants enrolled in the Botswana Mpepu study were randomized to prophylactic CTX or placebo between 14 and 34 days of life and continued through 15 months...
November 2017: Journal of the International AIDS Society
https://www.readbyqxmd.com/read/29073317/the-diagnostic-yield-of-noninvasive-microbiologic-sputum-sampling-in-a-cohort-of-patients-with-clinically-diagnosed-hospital-acquired-pneumonia
#10
Elliot L Naidus, Mary T Lasalvia, Edward R Marcantonio, Shoshana J Herzig
The clinical predictors of positive sputum culture have not been previously reported in hospital-acquired pneumonia (HAP), and data on yield of sputum culture in this setting are scant. Current Infectious Disease Society of America guidelines for HAP recommend noninvasive sputum sampling, though the data for this practice are limited. We assessed the yield of sputum culture in HAP cases at an academic medical center from January 2007 to July 2013. HAP cases were identifi ed by International Classifi cation of Diseases, Ninth Revision-Clinical Modifi cation codes for bacterial pneumonia and all cases were validated by chart review...
October 18, 2017: Journal of Hospital Medicine: An Official Publication of the Society of Hospital Medicine
https://www.readbyqxmd.com/read/29071076/burden-of-bacterial-upper-respiratory-tract-pathogens-in-school-children-of-nepal
#11
Sangita Thapa, Shishir Gokhale, Annavarapu Laxminarasimha Sharma, Lokendra Bahadur Sapkota, Shamshul Ansari, Rajendra Gautam, Sony Shrestha, Puja Neopane
INTRODUCTION: Respiratory tract infections are one of the most common human infections in all age group and important cause of mortality and morbidity worldwide. Most bacterial upper respiratory tract infections are vaccine preventable. This study aimed to determine the prevalence of carrier state of bacterial upper respiratory tract pathogens among school children. It also aimed to study their antibiograms. METHODS: The specimen from posterior pharyngeal wall and tonsils were collected from 204 participants on calcium alginate coated swabs (HiMedia)...
2017: BMJ Open Respiratory Research
https://www.readbyqxmd.com/read/29051689/detection-of-newly-defined-superantigenic-toxin-genes-and-coagulase-gene-polymorphism-in-staphylococcus-aureus-isolates
#12
Bareq A Al-Ajealy, Maysa S M Al-Shukri, Hassan S Al-Jumaily
The current study aims to use coagulase (coa) polymorphism gene to identify Staphylococcus aureus isolated from stool samples, evaluate the efficiency of these methods in discriminating variable strains, and compare these subtypes with antibiotypes. A total of 100 specimens were collected from patients in Babylon province, Iraq, between July 2016 and September 2016. Twenty S. aureus strains were isolated and identified using standard laboratory microbiological tests. The bacterial isolates were then examined by coa gene restriction fragment length polymorphism genotyping...
October 2017: Reviews in Medical Microbiology: a Journal of the Pathological Society of Great Britain and Ireland
https://www.readbyqxmd.com/read/29034852/comparison-of-direct-plating-and-broth-enrichment-culture-methods-for-detection-of-potential-bacterial-pathogens-in-respiratory-secretions
#13
Ravinder Kaur, Jareth Wischmeyer, Matthew Morris, Michael E Pichichero
OBJECTIVE: We compared the recovery of potential respiratory bacterial pathogens and normal flora from nasopharyngeal specimens collected from children during health and at the onset of acute otitis media (AOM) by selective direct-plating and overnight broth-enrichment. METHODS: Overall, 3442 nasal wash (NW) samples collected from young children were analysed from a 10-year prospective study. NWs were cultured by (1) direct-plating to TSAII/5 % sheep blood agar and chocolate agar plates and (2) overnight broth-enrichment in BacT/ALERT SA-broth followed by plating...
October 16, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29026631/salmonella-typhimurium-gastroenteritis-leading-to-chronic-prosthetic-vascular-graft-infection
#14
Milo Cullinan, Michael Clarke, Tim Dallman, Steven Peart, Deborah Wilson, Daniel Weiand
Introduction. It is estimated up to 6 % of prosthetic vascular grafts become infected. Staphylococcus aureus is predominant in early infection and coagulase-negative staphylococci are predominant in late infections. Enterobacteriaceae cause 14-40 % of prosthetic vascular graft infections. This is, to our knowledge the first reported case of Salmonella gastroenteritis causing chronic prosthetic vascular graft infection (PVGI). Case presentation. A 57 years old lady presented with signs and symptoms of prosthetic vascular graft infection...
August 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29026619/16s-rrna-deep-sequencing-identifies-actinotignum-schaalii-as-the-major-component-of-a-polymicrobial-intra-abdominal-infection-and-implicates-a-urinary-source
#15
Andrew Bryan, Lindsey M Kirkpatrick, John J Manaloor, Stephen J Salipante
Introduction. It can be difficult to catalogue the individual organisms comprising polymicrobial patient infections, both because conventional clinical microbiological culture does not facilitate the isolation and enumeration of all members of a complex microbial community, and because fastidious organisms may be mixed with organisms that grow rapidly in vitro. Empiric antimicrobial treatment is frequently employed based on the anatomical site and the suspected source of the infection, especially when an appropriately collected surgical specimen is not obtained...
May 2017: JMM Case Reports
https://www.readbyqxmd.com/read/28972874/impact-of-sequential-culture-results-on-diagnosis-and-de-escalation-of-the-antibiotic-regimen-in-joint-and-bone-infections
#16
Solen Kernéis, Cécile Leprince, Denis Archambeau, Luc Eyrolle, Philippe Leclerc, Hélène Poupet, Julien Loubinoux, Rémy Gauzit, Dominique Salmon, Odile Launay, Claire Poyart, Philippe Anract, Philippe C Morand
BACKGROUND: According to existing guidelines, orthopedic specimens collected in joint and bone infections (JBI) in our institution are cultured on several media sets and incubated for two, seven, and 14 days. The optimal timing for de-escalation of the first-line antibiotic combination according to the culture results needs to be defined. METHODS: Single-center, retrospective analysis of all adult patients with a first documented episode of JBI between May 2012 and April 2013...
November 2017: Surgical Infections
https://www.readbyqxmd.com/read/28966488/changing-prevalence-and-antibiotic-drug-resistance-pattern-of-pathogens-seen-in-community-acquired-pediatric-urinary-tract-infections-at-a-tertiary-care-hospital-of-north-india
#17
Vrushali Patwardhan, Dinesh Kumar, Varun Goel, Sarman Singh
AIMS AND OBJECTIVES: The aim and objective of this study was to assess the temporal changes in the microbiological profiles and antimicrobial resistance patterns of uropathogens in pediatric community-acquired UTI. MATERIALS AND METHODS: This is a retrospective analysis of data collected over a Scattered period of 5 years. The baseline data collected were from January to December 2009, and the second period considered for comparison was from January to December 2014...
October 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28951981/prospective-evaluation-of-a-high-multiplexing-real-time-polymerase-chain-reaction-array-for-the-rapid-identification-and-characterization-of-bacteria-causative-of-nosocomial-pneumonia-from-clinical-specimens-a-proof-of-concept-study
#18
S Roisin, T-D Huang, R de Mendonça, C Nonhoff, P Bogaerts, M Hites, B Delaere, S Hamels, F de Longueville, Y Glupczynski, O Denis
The purpose of this study was evaluation of the VAPChip assay based on the "Rapid-Array-PCR-technology" which targets 13 respiratory pathogens and 24 β-lactam resistance genes directly on respiratory clinical specimens. The first step included analysis of 45 respiratory specimens in order to calibrate and determine the threshold for target genes. The second prospective step involved 85 respiratory samples from patients suspected of nosocomial pneumonia collected in two academic hospitals over an 8-month period...
September 27, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28933357/risk-of-asymptomatic-bacteriuria-among-people-with-sickle-cell-disease-in-accra-ghana
#19
Eric S Donkor, Jonathan A Osei, Isaac Anim-Baidoo, Samuel Darkwah
Asymptomatic bacteriuria (ASB) is benign except in certain medical conditions such as pregnancy and immunosuppression. In Ghana, there are hardly any studies on urinary infections among sickle cell disease (SCD) patients, and the few studies carried out in Africa focused on pediatric SCD populations. The current study aimed to investigate the risk of ASB among SCD patients at a tertiary hospital in Ghana. This was a cross-sectional study involving 110 SCD patients and 110 age and sex matched healthy controls...
February 15, 2017: Diseases (Basel)
https://www.readbyqxmd.com/read/28929958/-screening-of-corynebacterium-diphtheriae-corynebacterium-ulcerans-and-corynebacterium-pseudotuberculosis-in-throat-swab-specimens-of-children-with-upper-respiratory-tract-infections
#20
Zeynep Gökçe Gayretli Aydın, Gönül Tanır, Selin Nar Ötgün, Meral Turan, Türkan Aydın Teke, Ayşe Kaman, Selçuk Kılıç
Although a significant decrease has been reported in the incidence of diphteria in many regions of the world following the routine diphtheria immunization programs, the emergence of new cases indicated that toxigenic strains are still circulating in the community. Diphtheria vaccine does not provide protection against asymptomatic carriage and colonization of non-toxigenic Corynebacterium diphtheriae. It is a known fact that invasive infections may arise from non-toxigenic C.diphtheriae strains that the non-toxigenic strains can become toxigenic strains leading to diphteria...
July 2017: Mikrobiyoloji Bülteni
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