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Microbiology specimen collection

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https://www.readbyqxmd.com/read/28323727/experience-with-the-use-of-single-use-disposable-bronchoscope-in-the-icu-in-a-tertiary-referral-center-of-singapore
#1
Dominic C Marshall, Rucha S Dagaonkar, Chan Yeow, Anura T Peters, Siew Kin Tan, Dessmon Y H Tai, Soon Keng Gohs, Albert Y H Lim, Benjamin Ho, Sennen J W Lew, John Abisheganaden, Akash Verma
BACKGROUND: Flexible bronchoscopy is performed frequently in intensive care units (ICUs) for various indications using the reusable conventional bronchoscope (CB). Recently, "single-use disposable bronchoscope" (SB) was introduced into the health care industry. The purpose of this study was to compare the utility of SB with CB in ICU. METHODS: A retrospective review of medical records of patients undergoing flexible bronchoscopy in the ICUs in the year 2015. RESULTS: Ninety-three patients undergoing flexible bronchoscopy in the ICU were studied...
April 2017: Journal of Bronchology & Interventional Pulmonology
https://www.readbyqxmd.com/read/28317567/oral-mucosal-infections-insights-into-specimen-collection-and-medication-management
#2
REVIEW
Arvind Babu Rajendra Santosh, Baddam Venkat Ramana Reddy
Oral mucosal infections appear as localized or generalized lesions. Symptoms range from almost unnoticeable lesions to severe pain. Systemic disease, age, immunocompromised condition, and medication use are common causes. Local causes include dentures, poor oral hygiene, traumatized epithelium, ulcerations, dentures, implants, oral piercing, and reduced salivary secretion. Oral mucosal infections are underdiagnosed and microbiological diagnosis should be more frequently used. Candidiasis is most frequently diagnosed...
April 2017: Dental Clinics of North America
https://www.readbyqxmd.com/read/28260599/peritonsillar-abscess-clinical-aspects-of-microbiology-risk-factors-and-the-association-with-parapharyngeal-abscess
#3
REVIEW
Tejs Ehlers Klug
PTA is a collection of pus located between the tonsillar capsule and the pharyngeal constrictor muscle. It is considered a complication of acute tonsillitis and is the most prevalent deep neck infection (approximately 2000 cases annually in Denmark) and cause of acute admission to Danish ENT departments. Teenagers and young adults are most commonly affected and males may predominate over females. However, no studies of age- and gender-stratified incidence rates have previously been published. Furthermore, smoking may be associated with increased risk of peritonsillar abscess (PTA) development, although the magnitude of the association has not been estimated...
March 2017: Danish Medical Journal
https://www.readbyqxmd.com/read/28257985/microbiological-aspects-of-fournier-s-gangrene
#4
Tuncay Yilmazlar, Baris Gulcu, Ozgen Isik, Ersin Ozturk
BACKGROUND: Fournier's gangrene (FG) is a devastating disease that is characterized by necrotizing fasciitis of the perineal, genital, or perianal region. Broad-spectrum antibiotics are the key component of its treatment. However, there is paucity of data regarding the optimal empirical antibiotherapy for FG. MATERIALS AND METHODS: Data from patients who underwent surgery for FG between January 2007 and December 2012 were retrieved from a prospectively collected departmental FG database...
February 28, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/28250261/high-prevalence-of-campylobacter-ureolyticus-in-stool-specimens-of-children-with-diarrhea-in-japan
#5
Noritoshi Hatanaka, Akinori Shimizu, Srinuan Somroop, Yiming Li, Masahiro Asakura, Akira Nagita, Sharda Prasad Awasthi, Atsushi Hinenoya, Shinji Yamasaki
Campylobacter ureolyticus has been considered as a potential pathogenic bacterium. In this study, a total 586 stool samples were collected from 0-12 year-old children with diarrhea between November 2013 and April 2015 and examined with microbiological tests in the hospital for diagnosis of common enteric pathogens including C. jejuni and C. coli. Then in our laboratory, these samples were analyzed by 16S rRNA sequence-based Campylobacter genus-specific PCR (C16S PCR) and 283 (48.3%) samples show positive with this PCR assay...
February 28, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28218980/microbiologic-yield-of-bronchoalveolar-lavage-specimens-from-stem-cell-transplant-recipients
#6
Kenneth K Sakata, Christine L Klassen, Kathryn B Bollin, Thomas E Grys, James L Slack, Lewis J Wesselius, Holenarasipur R Vikram
PURPOSE: Stem cell transplant (SCT) recipients commonly undergo bronchoalveolar lavage (BAL) collection as an infectious pulmonary work-up. Previous studies report the utility and overall diagnostic yield of fiberoptic bronchoscopy with BAL in this vulnerable population, though none focused purely on microbiologic yield or made comparisons with less invasive means of pathogen detection. We sought to determine and elaborate on the microbiologic yield of BAL in SCT recipients, assess a correlation between BAL studies and less invasive means of pathogen detection, and assess the utility of repeating a BAL within 30 days...
February 20, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28218003/virulotyping-of-shigella-spp-isolated-from-pediatric-patients-in-tehran-iran
#7
Reza Ranjbar, Masomeh Bolandian, Payam Behzadi
Shigellosis is a considerable infectious disease with high morbidity and mortality among children worldwide. In this survey the prevalence of four important virulence genes including ial, ipaH, set1A, and set1B were investigated among Shigella strains and the related gene profiles identified in the present investigation, stool specimens were collected from children who were referred to two hospitals in Tehran, Iran. The samples were collected during 3 years (2008-2010) from children who were suspected to shigellosis...
February 20, 2017: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/28186464/evaluation-of-antimicrobial-photodynamic-therapy-using-indocyanine-green-and-near-infrared-diode-laser-against-enterococcus-faecalis-in-infected-human-root-canals
#8
Charis Beltes, Nikolaos Economides, Hercules Sakkas, Chrissanthy Papadopoulou, Theodoros Lambrianidis
OBJECTIVE: Photodynamic therapy (PDT) is considered a promising adjunct to the currently available endodontic disinfection techniques leading to more effective reduction of intracanal bacteria. The present ex vivo study aimed to assess the antimicrobial effect of PDT using indocyanine green (ICG) as photosensitizer and a near-infrared (NIR) diode laser in root canals of human teeth infected with Enterococcus faecalis. MATERIALS AND METHODS: Ninety single-rooted teeth after chemomechanical preparation and sterilization were contaminated with an E...
February 9, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28149487/clinical-response-and-outcome-of-pneumonia-due-to-multi-drug-resistant-acinetobacter-baumannii-in-critically-ill-patients
#9
Lida Shojaei, Mostafa Mohammadi, Mohammad-Taghi Beigmohammadi, Mahsa Doomanlou, Alireza Abdollahi, Mohammad Mehdi Feizabadi, Hossein Khalili
BACKGROUND AND OBJECTIVES: The frequency of multi-drug resistant Acinetobacter spp. infections is increasing in Iran. Considering availability of limited therapeutic options, clinical response and outcome of ventilator-associated pneumonia due to multi-drug resistant A.baumannii were evaluated in critically ill patients. MATERIALS AND METHODS: In this prospective study, 29 patients with carbapenem resistance A. baumannii ventilator-associated pneumonia were enrolled...
October 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28139956/bacterial-microbiota-in-harbor-seals-phoca-vitulina-from-the-north-sea-of-schleswig-holstein-germany-around-the-time-of-morbillivirus-and-influenza-epidemics
#10
Ursula Siebert, Marion Rademaker, Sophie A Ulrich, Peter Wohlsein, Katrin Ronnenberg, Ellen Prenger-Berninghoff
We present microbiologic findings in harbor seal (phoca; Phoca vitulina ) carcasses collected from the North Sea of Schleswig-Holstein, Germany, 1996-2014, and interpret results in relation to potential variations caused by phocine distemper virus and influenza A virus mass mortalities. We conducted microbiologic investigations on 2,124 tissue samples from lung, liver, kidney, spleen, intestine, and mesenteric lymph nodes from 549 dead harbor seals of the German North Sea. A large variety of bacteria, including potentially pathogenic species such as Bordetella bronchiseptica , Brucella spp...
January 31, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28115402/surgical-resection-of-a-rare-cutaneous-manifestation-of-scedosporium-apiospermum-in-a-patient-who-underwent-renal-transplant
#11
A C S Stoneham, S E Stoneham, S A Wyllie, A N Pandya
A man aged 47 years who was immunosuppressed following renal transplantation for focal segmental glomerulosclerosis was referred to the Plastic Surgery team for management of a painful, chronic, granulomatous lesion of the right forearm. Serial ultrasound scans and MRI scans were not diagnostic, but microbiological specimens tested positive for the fungus Scedosporium apiospermum The renal transplant graft-which was failing-was removed, allowing him to cease immunosuppression. He then underwent a resection of the lesion and reconstruction with a split thickness skin graft...
January 23, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28115025/an-outbreak-of-ralstonia-pickettii-bloodstream-infection-associated-with-an-intrinsically-contaminated-normal-saline-solution
#12
Yin-Yin Chen, Wan-Tsuei Huang, Chia-Ping Chen, Shu-Mei Sun, Fu-Mei Kuo, Yu-Jiun Chan, Shu-Chen Kuo, Fu-Der Wang
OBJECTIVE Ralstonia pickettii has caused contamination of pharmaceutical solutions in many countries, resulting in healthcare infections or outbreak events. We determined the source of the outbreak of R. pickettii bloodstream infection (BSI). METHODS This study was conducted in a 3,000-bed tertiary referral medical center in Taiwan with >8,500 admissions during May 2015. Patients had been treated in the injection room or chemotherapy room at outpatient departments, emergency department, or hospital wards...
January 24, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28101156/clinical-and-microbiological-profile-of-babies-born-with-risk-of-neonatal-sepsis
#13
Bin Zhou, Xiao Liu, Jie-Bin Wu, Bao Jin, Yan-Yan Zhang
The aim of the present study was to evaluate the effects of antibiotics on the condition of babies born with risk of neonatal sepsis. From March, 2014 to February, 2015, 200 neonates born with risk factors of septicemia in the Neonatal Intensive Care Unit at Xuzhou Central Hospital, were enrolled in the present study. Venous blood samples were collected within 6 h of birth using aseptic technique. Part of the blood specimens were cultured using BACTEC PEDS PLUS/F Culture Vials. Subsequently, the subcultures were prepared from each presumptive positive vial and bacterial isolates were identified...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28077671/impact-of-long-term-care-facility-residence-on-the-antibiotic-resistance-of-urinary-tract-escherichia-coli-and-klebsiella
#14
Alicia Rosello, Andrew C Hayward, Susan Hopkins, Carolyne Horner, Dean Ironmonger, Peter M Hawkey, Sarah R Deeny
BACKGROUND: Long-term care facilities (LTCFs) are thought to be important reservoirs of antimicrobial-resistant (AMR) bacteria; however, there is no routine surveillance of resistance in LTCF residents, or large population-based studies comparing AMR in LTCFs with the community, so the relative burden of AMR in LTCFs remains unknown. OBJECTIVES: To compare the frequency of antibiotic resistance of urinary tract bacteria from residents of LTCFs for the elderly and adults aged 70 years or older living in the community...
January 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28043600/benefits-and-challenges-of-molecular-diagnostics-for-childhood-tuberculosis
#15
Cristina Gutierrez
Expanding tuberculosis (TB)-diagnostic services, including access to rapid tests, is a World Health Organization (WHO) strategy to accelerate progress toward ending TB. Faster and more sensitive molecular tests capable of diagnosing TB and drug-resistant TB have the technical capacity to address limitations associated with smears and cultures by increasing accuracy and shortening turnaround times as compared with those of these conventional laboratory methods. Nucleic acid amplification assays used to detect and analyze Mycobacterium tuberculosis (MTB)-complex nucleic acids can be used directly on specimens from patients suspected of having TB...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28018869/pathogens-and-antimicrobial-susceptibility-profiles-in-critically-ill-patients-with-bloodstream-infections-a-descriptive-study
#16
Rachel D Savage, Robert A Fowler, Asgar H Rishu, Sean M Bagshaw, Deborah Cook, Peter Dodek, Richard Hall, Anand Kumar, François Lamontagne, François Lauzier, John Marshall, Claudio M Martin, Lauralyn McIntyre, John Muscedere, Steven Reynolds, Henry T Stelfox, Nick Daneman
BACKGROUND: Surveillance of antimicrobial resistance is vital to guiding empirical treatment of infections. Collating and reporting routine data on clinical isolate testing may offer more timely information about resistance patterns than traditional surveillance network methods. METHODS: Using routine microbiology testing data collected from the Bacteremia Antibiotic Length Actually Needed for Clinical Effectiveness retrospective cohort study, we conducted a descriptive secondary analysis among critically ill patients in whom bloodstream infections had been diagnosed in 14 intensive care units (ICUs) in Canada...
October 2016: CMAJ Open
https://www.readbyqxmd.com/read/27941028/intra-abdominal-candida-spp-infection-in-acute-abdomen-in-a-quality-assurance-qa-certified-academic-setting
#17
Vito Rodolico, Paola Di Carlo, Gaspare Gulotta, Francesco D'Arpa, Giuseppe Salamone, Gianfranco Cocorullo, Antonino Agrusa, Anna Giammanco, Consolato Sergi
AIMS: To evaluate the contribution of light microscopy to detecting Candida spp infection in patients with complicated intra-abdominal infections (IAIs) admitted for acute abdomen to a quality assurance (QA)-certified surgical emergency ward. METHODS: We conducted a retrospective study (2008-2012) of 809 abdominal intraoperative or biopsy tissue specimens obtained from patients admitted with acute abdomen and microbiological samples positive for Candida spp. Demographic data, mortality, comorbidities, specimen type, microscopy results, special histological staining performed, antimicrobial therapy were collected and analysed...
December 9, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27894388/-analysis-of-distribution-and-drug-resistance-of-pathogens-isolated-from-541-hospitalized-children-with-burn-infection
#18
J X Dai, L Li, L Xu, Z H Chen, X Y Li, M Liu, Y Q Wen, X D Chen
Objective: To investigate the distribution and drug resistance of pathogens isolated from hospitalized children with burn infection. Methods: Totally 541 patients were hospitalized in Fujian Medical University Union Hospital, the 180th Hospital of Chinese People's Liberation Army(PLA), the 92th Hospital of PLA, and Longyan First Hospital from January 2013 to December 2015. Totally 848 clinical specimens (blood, catheter tip attachments, wound exudate, etc.) were collected for pathogen detection. After being identified by an automatic microbiological identification system, drug resistance of pathogens to 41 commonly-used antibiotics in clinic including gentamicin, aztreonam, erythromycin, clindamycin, etc...
November 20, 2016: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/27891182/case-control-study-of-diarrheal-disease-etiology-in-individuals-over-5%C3%A2-years-in-southwest-china
#19
Shun-Xian Zhang, Chun-Li Yang, Wen-Peng Gu, Lin Ai, Emmanuel Serrano, Pin Yang, Xia Zhou, Shi-Zhu Li, Shan Lv, Zhi-Sheng Dang, Jun-Hu Chen, Wei Hu, Li-Guang Tian, Jia-Xu Chen, Xiao-Nong Zhou
BACKGROUND: Acute diarrhea is one of the major public health problems worldwide. Most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in China. METHODS: 271 diarrhea cases and 149 healthy controls over 5 years were recruited from four participating hospitals between June 2014 and July 2015. Each stool specimen was collected to detect a series of enteric pathogens, involving five viruses (Rotavirus group A, RVA; Norovirus, NoV; Sapovirus, SaV; Astrovirus, As; and Adenovirus, Ad), seven bacteria (diarrheagenic Escherichia coli, DEC; non-typhoidal Salmonella, NTS; Shigella spp...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/27890455/the-detection-of-microbial-dna-but-not-cultured-bacteria-is-associated-with-increased-mortality-in-patients-with-suspected-sepsis-a-prospective-multi-centre-european-observational-study
#20
MULTICENTER STUDY
M J O'Dwyer, M H Starczewska, J Schrenzel, K Zacharowski, D J Ecker, R Sampath, D Brealey, M Singer, N Libert, M Wilks, J-L Vincent
OBJECTIVES: Blood culture results inadequately stratify the mortality risk in critically ill patients with sepsis. We sought to establish the prognostic significance of the presence of microbial DNA in the bloodstream of patients hospitalized with suspected sepsis. METHODS: We analysed the data collected during the Rapid Diagnosis of Infections in the Critically Ill (RADICAL) study, which compared a novel culture-independent PCR/electrospray ionization-mass spectrometry (ESI-MS) assay with standard microbiological testing...
March 2017: Clinical Microbiology and Infection
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