keyword
MENU ▼
Read by QxMD icon Read
search

Microbiology specimen collection

keyword
https://www.readbyqxmd.com/read/29051689/detection-of-newly-defined-superantigenic-toxin-genes-and-coagulase-gene-polymorphism-in-staphylococcus-aureus-isolates
#1
Bareq A Al-Ajealy, Maysa S M Al-Shukri, Hassan S Al-Jumaily
The current study aims to use coagulase (coa) polymorphism gene to identify Staphylococcus aureus isolated from stool samples, evaluate the efficiency of these methods in discriminating variable strains, and compare these subtypes with antibiotypes. A total of 100 specimens were collected from patients in Babylon province, Iraq, between July 2016 and September 2016. Twenty S. aureus strains were isolated and identified using standard laboratory microbiological tests. The bacterial isolates were then examined by coa gene restriction fragment length polymorphism genotyping...
October 2017: Reviews in Medical Microbiology: a Journal of the Pathological Society of Great Britain and Ireland
https://www.readbyqxmd.com/read/29034852/comparison-of-direct-plating-and-broth-enrichment-culture-methods-for-detection-of-potential-bacterial-pathogens-in-respiratory-secretions
#2
Ravinder Kaur, Jareth Wischmeyer, Matthew Morris, Michael E Pichichero
OBJECTIVE: We compared the recovery of potential respiratory bacterial pathogens and normal flora from nasopharyngeal specimens collected from children during health and at the onset of acute otitis media (AOM) by selective direct-plating and overnight broth-enrichment. METHODS: Overall, 3442 nasal wash (NW) samples collected from young children were analysed from a 10-year prospective study. NWs were cultured by (1) direct-plating to TSAII/5 % sheep blood agar and chocolate agar plates and (2) overnight broth-enrichment in BacT/ALERT SA-broth followed by plating...
October 16, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29026631/salmonella-typhimurium-gastroenteritis-leading-to-chronic-prosthetic-vascular-graft-infection
#3
Milo Cullinan, Michael Clarke, Tim Dallman, Steven Peart, Deborah Wilson, Daniel Weiand
Introduction. It is estimated up to 6 % of prosthetic vascular grafts become infected. Staphylococcus aureus is predominant in early infection and coagulase-negative staphylococci are predominant in late infections. Enterobacteriaceae cause 14-40 % of prosthetic vascular graft infections. This is, to our knowledge the first reported case of Salmonella gastroenteritis causing chronic prosthetic vascular graft infection (PVGI). Case presentation. A 57 years old lady presented with signs and symptoms of prosthetic vascular graft infection...
August 2017: JMM Case Reports
https://www.readbyqxmd.com/read/29026619/16s-rrna-deep-sequencing-identifies-actinotignum-schaalii-as-the-major-component-of-a-polymicrobial-intra-abdominal-infection-and-implicates-a-urinary-source
#4
Andrew Bryan, Lindsey M Kirkpatrick, John J Manaloor, Stephen J Salipante
Introduction. It can be difficult to catalogue the individual organisms comprising polymicrobial patient infections, both because conventional clinical microbiological culture does not facilitate the isolation and enumeration of all members of a complex microbial community, and because fastidious organisms may be mixed with organisms that grow rapidly in vitro. Empiric antimicrobial treatment is frequently employed based on the anatomical site and the suspected source of the infection, especially when an appropriately collected surgical specimen is not obtained...
May 2017: JMM Case Reports
https://www.readbyqxmd.com/read/28972874/impact-of-sequential-culture-results-on-diagnosis-and-de-escalation-of-the-antibiotic-regimen-in-joint-and-bone-infections
#5
Solen Kernéis, Cécile Leprince, Denis Archambeau, Luc Eyrolle, Philippe Leclerc, Hélène Poupet, Julien Loubinoux, Rémy Gauzit, Dominique Salmon, Odile Launay, Claire Poyart, Philippe Anract, Philippe C Morand
BACKGROUND: According to existing guidelines, orthopedic specimens collected in joint and bone infections (JBI) in our institution are cultured on several media sets and incubated for two, seven, and 14 days. The optimal timing for de-escalation of the first-line antibiotic combination according to the culture results needs to be defined. METHODS: Single-center, retrospective analysis of all adult patients with a first documented episode of JBI between May 2012 and April 2013...
October 3, 2017: Surgical Infections
https://www.readbyqxmd.com/read/28966488/changing-prevalence-and-antibiotic-drug-resistance-pattern-of-pathogens-seen-in-community-acquired-pediatric-urinary-tract-infections-at-a-tertiary-care-hospital-of-north-india
#6
Vrushali Patwardhan, Dinesh Kumar, Varun Goel, Sarman Singh
AIMS AND OBJECTIVES: The aim and objective of this study was to assess the temporal changes in the microbiological profiles and antimicrobial resistance patterns of uropathogens in pediatric community-acquired UTI. MATERIALS AND METHODS: This is a retrospective analysis of data collected over a Scattered period of 5 years. The baseline data collected were from January to December 2009, and the second period considered for comparison was from January to December 2014...
October 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28951981/prospective-evaluation-of-a-high-multiplexing-real-time-polymerase-chain-reaction-array-for-the-rapid-identification-and-characterization-of-bacteria-causative-of-nosocomial-pneumonia-from-clinical-specimens-a-proof-of-concept-study
#7
S Roisin, T-D Huang, R de Mendonça, C Nonhoff, P Bogaerts, M Hites, B Delaere, S Hamels, F de Longueville, Y Glupczynski, O Denis
The purpose of this study was evaluation of the VAPChip assay based on the "Rapid-Array-PCR-technology" which targets 13 respiratory pathogens and 24 β-lactam resistance genes directly on respiratory clinical specimens. The first step included analysis of 45 respiratory specimens in order to calibrate and determine the threshold for target genes. The second prospective step involved 85 respiratory samples from patients suspected of nosocomial pneumonia collected in two academic hospitals over an 8-month period...
September 27, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28933357/risk-of-asymptomatic-bacteriuria-among-people-with-sickle-cell-disease-in-accra-ghana
#8
Eric S Donkor, Jonathan A Osei, Isaac Anim-Baidoo, Samuel Darkwah
Asymptomatic bacteriuria (ASB) is benign except in certain medical conditions such as pregnancy and immunosuppression. In Ghana, there are hardly any studies on urinary infections among sickle cell disease (SCD) patients, and the few studies carried out in Africa focused on pediatric SCD populations. The current study aimed to investigate the risk of ASB among SCD patients at a tertiary hospital in Ghana. This was a cross-sectional study involving 110 SCD patients and 110 age and sex matched healthy controls...
February 15, 2017: Diseases (Basel)
https://www.readbyqxmd.com/read/28929958/-screening-of-corynebacterium-diphtheriae-corynebacterium-ulcerans-and-corynebacterium-pseudotuberculosis-in-throat-swab-specimens-of-children-with-upper-respiratory-tract-infections
#9
Zeynep Gökçe Gayretli Aydın, Gönül Tanır, Selin Nar Ötgün, Meral Turan, Türkan Aydın Teke, Ayşe Kaman, Selçuk Kılıç
Although a significant decrease has been reported in the incidence of diphteria in many regions of the world following the routine diphtheria immunization programs, the emergence of new cases indicated that toxigenic strains are still circulating in the community. Diphtheria vaccine does not provide protection against asymptomatic carriage and colonization of non-toxigenic Corynebacterium diphtheriae. It is a known fact that invasive infections may arise from non-toxigenic C.diphtheriae strains that the non-toxigenic strains can become toxigenic strains leading to diphteria...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28914110/evaluation-of-dual-target-specific-real-time-pcr-for-the-detection-of-kingella-kingae-in-a-danish-paediatric-population
#10
Victoria Elizabeth de Knegt, Gitte Qvist Kristiansen, Kristian Schønning
BACKGROUND: We aimed to evaluate the relevance of dual target real-time polymerase chain (PCR) assays targeting the rtxA and cpn60 genes of the paediatric pathogen Kingella kingae. We also studied for the first time the clinical and epidemiological features of K. kingae infections in a Danish population. METHOD: Children with K. kingae-positive cultures were identified from 11,477 children and 86 children younger than 16 years old from whom blood cultures and joint fluid cultures were obtained between January 2010 and November 2016...
September 15, 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28861140/microbiology-education-in-nursing-practice
#11
Robert J Durrant, Alexa K Doig, Rebecca L Buxton, JoAnn P Fenn
Nurses must have sufficient education and training in microbiology to perform many roles within clinical nursing practice (e.g., administering antibiotics, collecting specimens, preparing specimens for transport and delivery, educating patients and families, communicating results to the healthcare team, and developing care plans based on results of microbiology studies and patient immunological status). It is unclear whether the current microbiology courses required of nursing students in the United States focus on the topics that are most relevant to nursing practice...
2017: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/28855761/effect-of-conventional-irrigation-and-photoactivated-disinfection-on-enterococcus-faecalis-in-root-canals-an-in-vitro-study
#12
Niyati Balakrishna, Prashant Moogi, G Vinay Kumar, B R Prashanth, Nithin Kumar Shetty, Kaushal R Rao
AIMS: A study was done to evaluate the antimicrobial efficacy of sodium hypochlorite (NaOCl) and photoactivated disinfection (PAD) on Enterococcus faecalis. SETTINGS AND DESIGN: Random sampling, in-vitro study. SUBJECTS AND METHODS: Access opening and biomechanical preparation were performed on fifty freshly extracted mandibular second premolars. The specimens were sterilized; 15 μm of E. faecalis was inoculated into each canal and incubated at 36°C for 24 h...
March 2017: Journal of Conservative Dentistry: JCD
https://www.readbyqxmd.com/read/28816188/assessment-of-changes-in-nickel-and-chromium-levels-in-the-gingival-crevicular-fluid-during-fixed-orthodontic-treatment
#13
Vinny Bhasin, Swati J Pustake, Viprat Joshi, Anil Tiwari, Meenakshi Bhasin, Ramandeep S Punia
BACKGROUND: Various components of appliances used in fixed orthodontic treatment are fabricated from materials that are highly resistant in nature and have high strength and biocompatibility. Corrosion of materials occurs inside the oral cavity due to numerous environmental or oral factors that act on them. These factors include temperature, pH variation, salivary conditions, mechanical loads, microbiological and enzymatic activity, and various food components. Gingival crevicular fluid (GCF) is the material obtained from the gingival sulcus and might act as a potential source for various biomarkers in the orthodontic setup because inflammatory-induced response is directly related to orthodontic forces in GCF...
August 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28794179/evaluation-of-copan-fecalswab-as-specimen-type-for-use-in-xpert-c-difficile-assay
#14
Michael J Mashock, Matthew L Faron, Blake W Buchan, Nathan A Ledeboer
Liquid-based microbiology (LBM) devices incorporating flocked swabs and preservation medium ease transport of specimens and improve specimen yield compared to traditional fiber wound swabs; however, the performance of LBM collection devices has not been evaluated in many molecular assays. It is unclear how the differences in matrix and specimen loading with an LBM device will affect test performance compared to traditional collection devices. The purpose of this study was to evaluate the performance of specimens collected in FecalSwab transport medium (Copan Diagnostics, Murrieta, CA) compared to unpreserved stool using the Cepheid Xpert C...
October 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28774863/will-co-trimoxazole-resistance-rates-ever-go-down-resistance-rates-remain-high-despite-decades-of-reduced-co-trimoxazole-consumption
#15
Koen B Pouwels, Rahul Batra, Amita Patel, Jonathan D Edgeworth, Julie V Robotham, Timo Smieszek
OBJECTIVE: Several studies showed that a substantial decline in the use of co-trimoxazole did not result in a decline in resistance rates among Escherichia coli isolates. Since mathematical models have shown that it may take decades before resistance rates start to decline to relevant levels, we performed a new analysis using more recently collected data. METHODS: Data were extracted from Guy's and St Thomas' Hospitals Transmission and Antimicrobial Record database which contains microbiological test results from all specimens tested between 2002 and 2014...
July 31, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28719310/severity-of-pneumonia-in-under-5-year-old-children-from-developing-countries-a-multicenter-prospective-observational-study
#16
MULTICENTER STUDY
Thomas Bénet, Valentina Sanchez Picot, Shally Awasthi, Nitin Pandey, Ashish Bavdekar, Anand Kawade, Annick Robinson, Mala Rakoto-Andrianarivelo, Maryam Sylla, Souleymane Diallo, Graciela Russomando, Wilma Basualdo, Florence Komurian-Pradel, Hubert Endtz, Philippe Vanhems, Gláucia Paranhos-Baccalà, For The Gabriel Network
Pneumonia is the leading cause of death in children. The objectives were to evaluate the microbiological agents linked with hypoxemia in hospitalized children with pneumonia from developing countries, to identify predictors of hypoxemia, and to characterize factors associated with in-hospital mortality. A multicenter, observational study was conducted in five hospitals, from India (Lucknow, Vadu), Madagascar (Antananarivo), Mali (Bamako), and Paraguay (San Lorenzo). Children aged 2-60 months with radiologically confirmed pneumonia were enrolled prospectively...
July 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28709485/biofilm-colonization-of-mycobacterium-abscessus-new-threat-in-hospital-acquired-surgical-site-infection
#17
Roumi Ghosh, Soumen Das, Harish Kela, Asmita De, Jayeeta Haldar, Prasanta Kumar Maiti
INTRODUCTION: Rapidly growing non-tuberculous mycobacteria (NTM) are hazardous cause of post-operative soft tissue infection leading to nosocomial outbreaks following various surgical procedures, especially laparoscopic surgeries using heat sensitive, non-autoclavable surgical instruments. METHODOLOGY: Surgery department of our hospital noticed increase in rate of post-laparoscopic abdominal port site infection (PSI) and informed the Microbiology Department. A prospective investigational study of defined cases with the aim of source tracing and formulation of infection control measures was initiated...
July 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28669830/drivers-and-impact-of-antifungal-therapy-in-critically-ill-patients-with-aspergillus-positive-respiratory-tract-cultures
#18
J A Paiva, P Mergulhão, A Gomes, F S Taccone, A-M Van den Abeele, P Bulpa, B Misset, W Meersseman, G Dimopoulos, J Rello, D Vogelaers, S Blot
Invasive pulmonary aspergillosis (IPA) is an increasingly recognised problem in critically ill patients. Little is known about how intensivists react to an Aspergillus-positive respiratory sample or the efficacy of antifungal therapy (AFT). This study aimed to identify drivers of AFT prescription and diagnostic workup in patients with Aspergillus isolation in respiratory specimens as well as the impact of AFT in these patients. ICU patients with an Aspergillus-positive respiratory sample from the database of a previous observational, multicentre study were analysed...
October 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28669567/nationwide-surveillance-of-bacterial-respiratory-pathogens-conducted-by-the-surveillance-committee-of-japanese-society-of-chemotherapy-the-japanese-association-for-infectious-diseases-and-the-japanese-society-for-clinical-microbiology-in-2012-general-view-of
#19
Katsunori Yanagihara, Akira Watanabe, Nobuki Aoki, Tetsuya Matsumoto, Masaki Yoshida, Junko Sato, Tomotaro Wakamura, Keisuke Sunakawa, Junichi Kadota, Hiroshi Kiyota, Satoshi Iwata, Mitsuo Kaku, Hideaki Hanaki, Yoshinobu Ohsaki, Satoru Fujiuchi, Manabu Takahashi, Kenichi Takeuchi, Hiroaki Takeda, Hideki Ikeda, Makoto Miki, Susumu Nakanowatari, Hiroshi Takahashi, Mutsuko Utagawa, Hajime Nishiya, Sayoko Kawakami, Eriko Morino, Jin Takasaki, Kazuhisa Mezaki, Naohiko Chonabayashi, Chie Tanaka, Hideko Sugiura, Hajime Goto, Takeshi Saraya, Daisuke Kurai, Yasuhiro Katono, Rika Inose, Yoshihito Niki, Takahiro Takuma, Makoto Kudo, Shigeru Ehara, Yoshimi Sato, Hiroki Tsukada, Nobuei Watabe, Yasuo Honma, Hiroshige Mikamo, Yuka Yamagishi, Atsushi Nakamura, Minoru Ohashi, Masafumi Seki, Shigeto Hamaguchi, Masahiro Toyokawa, Yasunori Fujikawa, Noriko Mitsuno, Akira Ukimura, Takayuki Miyara, Takahito Nakamura, Keiichi Mikasa, Kei Kasahara, Koji Ui, Saori Fukuda, Akihiro Nakamura, Mika Morimura, Mikio Yamashita, Yoshio Takesue, Yasunao Wada, Keisuke Sugimoto, Nobuchika Kusano, Motoko Nose, Eiichirou Mihara, Masao Kuwabara, Masao Doi, Yaeko Watanabe, Hirokazu Tokuyasu, Satoshi Hino, Kiyoshi Negayama, Hiroshi Mukae, Toshinori Kawanami, Toshiyuki Ota, Masaki Fujita, Junichi Honda, Kazufumi Hiramatsu, Yosuke Aoki, Mami Fukuoka, Hiroki Magarifuchi, Zenzo Nagasawa, Norihito Kaku, Jiro Fujita, Futoshi Higa, Masao Tateyama
The nationwide surveillance on antimicrobial susceptibility of bacterial respiratory pathogens from the patients in Japan was conducted by Japanese Society of Chemotherapy, Japanese association for infectious diseases and Japanese society for Clinical Microbiology in 2012. The isolates were collected from clinical specimens obtained from well-diagnosed adult patients with respiratory tract infections during the period between January and December in 2012 by three societies. Antimicrobial susceptibility testing was conducted at the central reference laboratory according to the method recommended by Clinical Laboratory Standard Institutes...
September 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28596960/acinetobacter-baumannii-infection-in-transfusion-dependent-thalassemia-patients-with-sepsis
#20
Muzaheed Abdul Rasheed, Faisal Mousa Alzahrani, Saeed Sattar Shaikh
PURPOSE: To identify the Acinetobacter baumannii infection among transfusion dependent thalassemia patients. METHODS: A quantitative approach was employed to assess Acinetobacter baumannii infection in transfusion dependent thalassemia patients. Samples were collected from 916 patients, which have shown bacterial growth on MacConkey and blood agar culture media. A. baumannii strains were identified by microbiological methods and Gram's staining. API 20 E kit (Biomerieux, USA) was used for final identification...
2017: BioMed Research International
keyword
keyword
103812
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"