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Chainarong Navanukraw, Vilaivan Khanthusaeng, Aree Kraisoon, Suthipong Uriyapongson
The study was conducted to compare estrous rate, ovulatory response, plasma progesterone concentrations, and conception rate following cervical artificial insemination in goats given a new or once-used controlled internal drug release (CIDR) device with human chorionic gonadotropin (hCG). Fifty-six Thai-native goats with the average age and body weight of 11 months and 17.3 kg received a 14-day treatment with a new CIDR device (Eazi-Breed(TM)CIDR®, Pfizer, NY, USA) or a once-used CIDR device. All goats received a 300-IU injection of hCG (Chorulon®, Intervet International B...
December 2014: Tropical Animal Health and Production
A B Nascimento, R W Bender, A H Souza, H Ayres, R R Araujo, J N Guenther, R Sartori, M C Wiltbank
Reproductive management programs that synchronize ovulation can ovulate a smaller than normal follicle, potentially resulting in inadequate progesterone (P4) concentrations after artificial insemination (AI). Ovulation of the dominant follicle of the first follicular wave with human chorionic gonadotropin (hCG) treatment can produce an accessory corpus luteum and increase circulating P4 concentrations. This manuscript reports the results of 2 separate analyses that evaluated the effect of hCG treatment post-AI on fertility in lactating dairy cows...
May 2013: Journal of Dairy Science
J O Giordano, M C Wiltbank, J N Guenther, M S Ares, G Lopes, M M Herlihy, P M Fricke
Our objectives were to (1) compare the effect on pregnancies per artificial insemination (P/AI) of presynchronization of the estrous cycle with human chorionic gonadotropin (hCG) 7d before resynchronization of ovulation (Resynch) initiated 25 d after timed artificial insemination (TAI) and compare the presynchronization treatment with the Double-Ovsynch (DO) protocol, and (2) evaluate whether hCG for presynchronization could be replaced with GnRH. In experiment 1, lactating Holstein cows were blocked by parity and were randomly assigned to receive (1) Resynch-25 (D25), the Resynch protocol (GnRH-7 d-PGF(2 α)-56 h-GnRH-16 h-TAI) initiated 25 d after TAI (n=418); (2) HGPG, presynchronization with hCG (2,000 IU of Chorulon) 7d before D25 (n=450); and (3) DO (Pre-Resynch, GnRH-7 d-PGF(2 α)-72 h-GnRH; Breeding-Resynch, GnRH-7 d-PGF(2 α)-56 h-GnRH-16 h-TAI) initiated 22d after TAI (n=405)...
October 2012: Journal of Dairy Science
M Saleh, M Shahin, W Wuttke, M Gauly, W Holtz
The present investigation addresses the pharmacokinetics of human chorionic gonadotropin (hCG), intramuscularly (i.m.) administered to goats. Nine pluriparous does of the Boer goat breed, 2-6 years of age and weighing 45-60 kg, were administered 500 IU hCG (2 ml Chorulon) deep into the thigh musculature 18 h after superovulatory FSH treatment. Blood samples were drawn from the jugular vein at 2  h intervals for the first 24h, at 6 h intervals until 42 h, and at 12 h intervals until 114 h after administration...
July 2012: Reproduction: the Official Journal of the Society for the Study of Fertility
B Rostami, A Niasari-Naslaji, M Vojgani, D Nikjou, H Amanlou, A Gerami
The purpose of the present study was to hasten the resumption of ovarian activity early postpartum in lactating dairy cows, using equine chorionic gonadotropin (eCG), to enhance follicular growth, followed by hCG, to induce ovulation. Primiparous Holstein dairy cows (n=21) were assigned equally into eCG, eCG-hCG and Control groups. Cows in the eCG and eCG-hCG groups received an i.m. injection of eCG (500 IU Folligon®) on Day 6 postpartum. Cows in the eCG-hCG group were also given an i.m. injection of hCG (500 IU Chorulon®), once dominant follicle reached the diameter of 13-16 mm following eCG injection...
October 2011: Animal Reproduction Science
A N Al Yacoub, M Gauly, B Sohnrey, W Holtz
The aim of this investigation was to optimize fixed-time insemination in goats by clustering ovulations in prostaglandin F(2α)-synchronized goats either with gonadotropin releasing hormone (GnRH) or human chorionic gonadotropin (hCG). The underlying intention was to reduce the incidence of short cycles by providing a more sustained stimulation of the corpus luteum by substituting the commonly used GnRH with longer-acting hCG. It was conjectured that this might render the corpus luteum less prone to premature regression...
December 2011: Theriogenology
Craig A Watson, Jeffrey E Hill, Jeffrey Scott Graves, Amy L Wood, Kathy Heym Kilgore
The spotted green pufferfish (Tetraodon nigroviridis) is an important genetics model animal due to its small, well-mapped genome. However, only wild-caught juveniles and adults are available to researchers. A lack of gametes, fertilized eggs, developing embryos, and other early life stages hampers development of the full potential of T. nigroviridis as a model research species. We report on successful spawning trials using a novel induced spawning technique, ovarian lavage. Chorulon(®) (human chorionic gonadotropin, hCG) was injected into a catheter inserted into the oviduct at a rate of 3 μl/g body weight...
June 2009: Marine Genomics
R C Rossetti, A Perdigão, F S Mesquita, M Sá Filho, G P Nogueira, R Machado, C M B Membrive, M Binelli
The objective was to compare pharmacological strategies aiming to inhibit prostaglandin F2 alpha (PGF(2α)) synthesis (flunixin meglumine; FM), stimulate growth of the conceptus (recombinant bovine somatotropin; bST) and progesterone (P(4)) synthesis (human chorionic gonadotropin; hCG), as well as their combinations, regarding their ability to improve pregnancy rates in beef cattle. Lactating Nelore cows (N = 975), 35 to 70 days postpartum, were synchronized and inseminated by timed artificial insemination (TAI) on Day 0...
September 1, 2011: Theriogenology
C R Dahlen, S L Bird, C A Martel, Kc Olson, J S Stevenson, G C Lamb
We determined the effects of administering hCG 7 d after a fixed-time AI (TAI) on ovarian response, concentrations of progesterone, and pregnancy rates in postpartum suckled beef cows. Cows at 6 locations received 100 microg of GnRH (Fertagyl, Intervet Animal Health, Millsboro, DE) and a controlled internal drug release (CIDR) device (CIDR EAZI-Breed, Pfizer Animal Health, New York, NY), followed in 7 d by 25 mg of PGF(2alpha) (Lutalyse, Pfizer Animal Health) and CIDR removal. At 64 h after CIDR removal, cows received an injection of GnRH and AI (d 0), and then were stratified by days postpartum and parity and assigned randomly to 2 treatments administered 7 d after TAI: 1) 1 mL of saline (saline; n = 252); or 2) 1,000 IU of hCG (Chorulon, Intervet Animal Health; n = 254)...
July 2010: Journal of Animal Science
T H Khan, N F G Beck, M Khalid
The objectives of this study were to determine the effect of a single intramuscular injection of 200IU hCG (Chorulon) on Day 12 post-mating on ovarian function and subsequent lambing performance in ewes and ewe lambs bred at synchronised oestrus during the breeding season and on the lambing performance of ewes induced to breed during late anoestrus. All animals were mated to rams at synchronised oestrus and on Day 12 post-mating given normal saline or 200IU hCG. In Experiment 1, laparoscopic results showed that hCG treatment induced accessory corpora lutea in ewes (control=0/7; hCG=5/7) but not in ewe lambs (control=0/7; hCG=0/7)...
November 2009: Animal Reproduction Science
N Shams-Esfandabadi, A Shirazi, P Mirshokrai, M Bonyadian
The objectives of this study were to determine the effect of administration of exogenous hCG 5 days after Artificial Insemination (AI) on serum progesterone concentration and conception rate in lactating dairy cows. In this experiment 5 days after AI the cows were alternately assigned randomly to treatment group (n=70) received an injection of 3000 IU hCG (Chorulon, each vial contained 1500 IU, Intervet, Holland) and control group (n=88), received no treatment. On day 5 and 12 blood samples from 20 of cows (10 in each group) were obtained to measure serum progesterone concentrations...
August 15, 2007: Pakistan Journal of Biological Sciences: PJBS
J R Clulow, H Buss, H Sieme, J A Rodger, A J Cawdell-Smith, G Evans, D Rath, L H A Morris, W M C Maxwell
In the 2004/2005 breeding season, the fertility of sex-sorted (SS) and non-sorted (NS) frozen stallion spermatozoa from two Hannovarian stallions was compared. A hysteroscopic insemination technique [Morris, L.H., Tiplady, C., Allen, W.R., 2003a. Pregnancy rates in mares after a single fixed time hysteroscopic insemination of low numbers of frozen-thawed spermatozoa onto the uterotubal junction. Equine Vet. J. 35, 197-201] was used to deposit low doses (6, 13 or 25 x 10(6) frozen-thawed SS or NS spermatozoa) onto the utero-tubal junction at 32 or 38 h after the administration of Chorulon (2500 IU, Intervet)...
November 2008: Animal Reproduction Science
T H Khan, N F G Beck, M Khalid
The objectives of this study were to determine the effect of GnRH analogue (buserelin) or human chorionic gonadotrophin (hCG, Chorulon) treatment on Day 12 of pregnancy on ovarian function, plasma hormone concentrations, conceptus growth and placentation in ewes and ewe lambs. After oestrus synchronization with progestagen sponges and eCG, all the animals were mated with fertile rams. Both ewes and ewe lambs (20 per treatment group) were given either normal saline or 4 microg GnRH or 200 IU hCG on Day 12 post-mating...
December 2007: Animal Reproduction Science
Jeffrey R Sommer, E Bruce Collins, Jose L Estrada, Robert M Petters
An efficient protocol was developed to synchronize and superovulate mature pigs for the collection of pronuclear stage embryos suitable for DNA microinjection. A timed and coordinated regimen of Lutalyse, PG600 and Chorulon along with daily checking for estrus allowed synchronization of groups of gilts having estrous cycles at regular intervals. Pigs 10-16 days after the beginning of standing estrus have been successfully synchronized into estrus using this protocol. A standard dose of each drug was used independent of size or age of the animal...
August 2007: Animal Reproduction Science
A K Goel, K P Agrawal
Superovulatory response and embryo production efficacy were investigated in adult (age 2-4 years, average body weight: 27-43 kg) cycling Jakhrana goats (n = 15) under semi-arid environmental conditions of India by administering different superovulatory regimens. Goats were reared under semi-intensive system of management in established farm conditions. To synchronize oestrus, a luteolytic dose of carboprost tromethamine (Upjohn, UK) was administered intramuscularly to all does at the dose rate of 5 microg per kg body weight in a double dose schedule with an interval of 11 days...
October 2005: Tropical Animal Health and Production
Manzoor A Nowshari, Syed A Ali
The purpose of the present investigation was to study the extent to which season and the gonadotropin preparation interferes with the superovulatory response in the dromedary. Adult camels were treated for superovulation during the breeding (November to April) and non-breeding season (May to October). Animals were synchronized by daily i.m. injections of progesterone (125 mg/animal/day, Jurox, UK) for 10 to 14 days. Superovulation was induced by 400mg pFSH alone (Follitropin V, Vetrepharm, Canada) administered in eight descending doses at 12h intervals or a combination of PMSG (2000IU, Folligon, Intervet, The Netherlands), injected with last injection of progesterone and 400mg pFSH in eight descending doses...
October 15, 2005: Theriogenology
L H A Morris, C Tiplady, W R Allen
REASONS FOR PERFORMING STUDY: To compensate for the wide variation in the freezability of stallion spermatozoa, it has become common veterinary practice to carry out repeated ultrasonography of the ovaries of oestrous mares in order to be able to inseminate them within 6-12 h of ovulation with a minimum of 300-500 x 10(6) frozen-thawed spermatozoa. Furthermore, in order to achieve satisfactory fertility, this requirement for relatively high numbers of spermatozoa currently limits our ability to exploit recently available artificial breeding technologies, such as sex-sorted semen, for which only 5-20 x 10(6) spermatozoa are available for insemination...
March 2003: Equine Veterinary Journal
S J Griffiths, T A Bramley, G S Menzies, D J Adams
An 18 kDa pregnancy urine protein preparation, purified to apparent electrophoretic homogeneity as judged by silver-staining of polyacrylamide gels, inhibited binding of 125I-hLH (human luteinizing hormone) to Candida albicans microsomes, reacted with monoclonal and polyclonal antibodies raised against human chorionic gonadotrophin (hCG) beta-core protein and exhibited ribonuclease (RNase) activity. Eleven of the 12 amino acids at the N-terminus of a protein in this preparation were identical to those of the N-terminus of human non-secretory ribonuclease...
October 31, 1997: Molecular and Cellular Endocrinology
L Kostov, G Radev, A Andreev
Experiments were carried out to raise the conception rate in sows during the summer season under industrial conditions of breeding. Eight test groups of 365 animals were formed as well as four control ones with 137 pigs. The sucking period lasted 28 to 33 days. When in estrus the sows were mated with boars twice at a 24-hour interval. In five groups that were dried up from July to September was recorded the effect of several kinds of gonadotropic hormones administered alone or in combination with vitamin E and steroid hormones -- Gravohormone Tolligon, an oil emulsion of PMS (500 IU), with 250 Chorulon and 2 mg estradiol benzoicum; an oil solution of PMS (500 IU) with 2 mg estradiol, 25 mg testosterone and 30 mg vitamin E...
1976: Veterinarno-meditsinski Nauki
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