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https://www.readbyqxmd.com/read/27596591/effects-of-repeated-comparative-intradermal-tuberculin-testing-on-test-results-a-longitudinal-study-in-tb-free-red-deer
#1
Azlan Che-Amat, Maria Ángeles Risalde, David González-Barrio, Jose Antonio Ortíz, Christian Gortázar
BACKGROUND: Diagnosing tuberculosis (TB) in farmed red deer (Cervus elaphus) is challenging and might require combining cellular and humoral diagnostic tests. Repeated skin-testing with mycobacterial purified protein derivatives (PPDs) might sensitize or desensitize the subjects to both kinds of diagnostic tools. We evaluated the effect of repeated (every 6 months) comparative tuberculin skin testing on skin test and ELISA responsiveness in farmed red deer hinds from a TB-free herd. Eighteen 8-month old hinds were inoculated with bovine and avian PPDs and the mitogen phytohaemagglutinin (PHA), as positive control and concurrently tested by ELISA for antibodies against avian (avian PPD, aPPD and protoplasmatic antigen 3, PPA3) and bovine antigens (bPPD and MPB70)...
2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27032499/oral-administration-of-heat-inactivated-mycobacterium-bovis-reduces-the-response-of-farmed-red-deer-to-avian-and-bovine-tuberculin
#2
Vladimir López, David González-Barrio, José Francisco Lima-Barbero, José Antonio Ortiz, Lucas Domínguez, Ramón Juste, Joseba M Garrido, Iker A Sevilla, Pilar Alberdi, José de la Fuente, Christian Gortázar
Orally delivered mycobacterial antigens may not sensitize the immunized animals causing a positive tuberculin skin test response. As the first step to address this critical issue, we characterized the response of farmed red deer (Cervus elaphus) to orally delivered heat-inactivated Mycobacterium bovis. Thirty-two adult red deer hinds from a farm known to be free of tuberculosis (TB) were randomly assigned to two different treatment groups, immunized (n=24) and control (n=8). Immunized hinds were dosed orally with 2 ml of PBS containing 6 × 10(6) heat-inactivated M...
April 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/26667540/evaluation-of-diagnostic-accuracy-of-the-comparative-tuberculin-skin-test-in-rehabilitant-bornean-orangutans-pongo-pygmaeus
#3
Rosalie Dench, Fransiska Sulistyo, Agus Fahroni, Joost Philippa
The tuberculin skin test (TST) has been the mainstay of tuberculosis (TB) testing in primates for decades, but its interpretation in orangutans (Pongo spp.) is challenging, because many animals react strongly, without evidence of infection with Mycobacterium tuberculosis complex. One explanation is cross-reactivity with environmental nontuberculous mycobacteria (NTM). The use of a comparative TST (CTST), comparing reactivity to avian (representing NTM) and bovine (representing tuberculous mycobacteria) tuberculins aids in distinguishing cross-reactivity due to sensitization by NTM from shared antigens...
December 2015: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/26525466/-the-efficacious-algorithm-for-the-diagnostics-and-treatment-of-benign-paroxysmal-positional-vertigo
#4
N L Kunel'skaya, A L Guseva, E V Baibakova, S D Chistov, M A Chugunova
The objective of the present study was to elucidate the prevalence of various forms of benign paroxysmal positional vertigo (BPPV) among the patients with the complaints of dizziness who had applied for the medical aid. The secondary objective was to evaluate the effectiveness of the newly developed algorithm for the diagnostics and treatment of various forms of the disease. A total of 195 patients with the verified BPPD diagnosis were available for the examination. The fraction of the patients visiting the otoneurologist's office with complaints of this conditions is estimated at 17...
2015: Vestnik Otorinolaringologii
https://www.readbyqxmd.com/read/26478942/metal-ion-complexes-of-n-n-bis-2-pyridylmethyl-trans-1-2-diaminocyclohexane-n-n-diacetic-acid-h2bpcd-cis-trans-isomerization-equilibria
#5
Jan Florián, Craig C McLauchlan, Daniel S Kissel, Chad C Eichman, Albert W Herlinger
The synthesis of N,N'-bis(2-pyridylmethyl)-trans-1,2-diaminocyclohexane-N,N'-diacetic acid (H2bpcd) and its complexation of Ga(III) and Co(III) are reported. H2bpcd and the metal-bpcd(2-) complexes, isolated as hexafluorophosphate salts, were characterized by elemental analysis, X-ray crystallography, IR spectroscopy, and (1)H and (13)C NMR spectroscopy. [Ga(bpcd)]PF6, [Ga(C22H26N4O4)]PF6, crystallized in the orthorhombic space group Ibca, with a = 13.8975(7) Å, b = 15.0872(7) Å, c = 22.2418(10) Å, and Z = 8...
November 2, 2015: Inorganic Chemistry
https://www.readbyqxmd.com/read/26288211/-the-complete-elimination-of-the-episodes-of-benign-positional-paroxysmal-vertigo-in-a-patient-with-the-involvement-of-the-posterior-semicircular-canal-following-the-eccentric-rotation-test
#6
É I Matsnev, E É Sigaleva
The objective of the present study was the clinical and neurophysiological analysis of the observations of a 42 year-old female patient suffering from benign paroxysmalpositional dizziness (BPPD) associated with the lesion of the posterior semicircular canal. The dynamic evaluation of the utricular function was performed during eccentric rotation of the patient in the vestibulometric chair at a speed of 300 degrees. The chair together with the patients was atomatically displaced during the rotation 3.5--4.0 cm to the right or left off the vertical axis, and the error in subjective perception of the deviation from the vertical was estimated...
2015: Vestnik Otorinolaringologii
https://www.readbyqxmd.com/read/26267100/short-communication-proteomic-characterization-of-tuberculin-purified-protein-derivative-from-mycobacterium-bovis
#7
Yun Sang Cho, Young-Boo Jang, Sang-Eun Lee, Je-Yoel Cho, Jung-Mo Ahn, Inyeong Hwang, Eunjeong Heo, Hyang-Mi Nam, Donghee Cho, Moon Her, Young Hwa Jean, Suk Chan Jung, Jong Man Kim, Hee Soo Lee, Keechan Lee, John T Belisle
Bovine tuberculin purified protein derivative (bPPD) is used as an intradermal test (IT) reagent to detect bovine tuberculosis (bTB) in most countries. Identification of bPPD proteins is critical to understanding the immunological reaction of IT at the molecular level. While bPPD from the United Kingdom (UK) and Brazil (BR) have been recently defined at the proteomic level, bPPD from the Republic of Korea (KR) has not yet been analyzed. Here, bPPD KR proteome was examined for the first time. In total, 271 proteins were identified, including Mycobacterium bovis-specific proteins Mb0854c and Mb2898, and 42 known T cell antigens...
August 2015: Research in Veterinary Science
https://www.readbyqxmd.com/read/25086394/synthesis-and-characterization-of-cationic-polymeric-nanoparticles-as-simvastatin-carriers-for-enhancing-the-osteogenesis-of-bone-marrow-mesenchymal-stem-cells
#8
Chau-Zen Wang, Yin-Chih Fu, Shih-Ciang Jian, Yan-Hsiung Wang, Po-Len Liu, Mei-Ling Ho, Chih-Kuang Wang
Simvastatin (SIM) can increase osteoblast activity and enhance osteogenesis. However, some limitations of SIM have been noted, such as statin-associated rhabdomyolysis and its poor solubility in water. In this study, we fabricated new cationic nanoparticles (NPs) designed for the controlled release of hydrophobic SIM and endocytosis by cells with the aim of reducing the total required amount of SIM administered and enhancing the osteogenesis of bone marrow mesenchymal stem cells (BMSCs). New copolymers of bis(poly(lactic-co-glycolic acid)-phenylalanine-polyethylene glycol)-quaternary ammonium grafted diethyltriamine (bis(PLGA-phe-PEG)-qDETA; BPPD) were created using a diethyltriamine-quaternary ammonium (qDETA) moiety, hetero-bifunctional polyethylene glycol (COOH-PEG-NH2), phenylalanine (phe) and poly(lactic-co-glycolic acid) (PLGA)...
October 15, 2014: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/24649926/metal-ion-complexes-of-n-n-bis-2-pyridylmethyl-1-3-diaminopropane-n-n-diacetic-acid-h2bppd
#9
Daniel S Kissel, Jan Florián, Craig C McLauchlan, Albert W Herlinger
A higher yield synthesis of N,N'-bis(2-pyridylmethyl)-1,3-diaminopropane-N,N'-diacetic acid (H2bppd) and its complexation of trivalent metal ions (Al(III), Ga(III), In(III)) and selected lanthanides (Ln(III)) are reported. H2bppd and the metal-bppd(2-) complexes, isolated as hexafluorophosphate salts, were characterized by elemental analysis, mass spectrometry, IR, and (1)H and (13)C NMR spectroscopy. [Ga(bppd)]PF6, [Ga(C19H22N4O4)]PF6, was crystallized as colorless needles by slow evaporation from anhydrous methanol; its molecular structure was solved by direct X-ray crystallography methods...
April 7, 2014: Inorganic Chemistry
https://www.readbyqxmd.com/read/23723791/-2-2-n-n-bis-pyridin-2-ylmeth-yl-propane-1-3-diyldi-nitrilo-di-acetato-cobalt-iii-hexa-fluoridophosphate-aceto-nitrile-0-064-solvate
#10
Craig C McLauchlan, Daniel S Kissel, William R Arnold, Albert W Herlinger
In the title compound, [Co(C19H22N4O4)]PF6·0.064CH3CN, commonly known as [Co(bppd)]PF6·0.064CH3CN, where bppd represents the historical ligand name N,N'-bis(2-pyridylmethyl)-1,3-diaminopropane-N,N'-diacetate, the Co(III) atom is coordinated in a distorted octa-hedral geometry with an N4O2 donor atom set. The acetate O atoms, which exhibit monodentate coordination, are oriented in a trans configuration with respect to each other, whereas the pyridyl N atoms are coordinated in a cis configuration. The compound crystallizes with two crystallographically unique cations and two anions per asymmetric unit along with a disordered, partially occupied (occupancy = 0...
May 1, 2013: Acta Crystallographica. Section E, Structure Reports Online
https://www.readbyqxmd.com/read/22523563/seroprevalence-and-risk-factors-associated-to-mycobacterium-bovis-in-wild-artiodactyl-species-from-southern-spain-2006-2010
#11
Ignacio García-Bocanegra, Bernat Pérez de Val, Antonio Arenas-Montes, Jorge Paniagua, Mariana Boadella, Christian Gortázar, Antonio Arenas
The control of bovine tuberculosis (bTB) is at a critical point in the last stage of eradication in livestock. Wildlife species recently have emerged infected with TB in Europe, particularly ungulates in the Iberian Peninsula. Epidemiological information regarding TB in wild ungulates including affected species, prevalence, associated risk factors and appropriate diagnostic methods need to be obtained in these countries. A cross-sectional study was carried out on wild artiodactyl species, including Eurasian wild boar (Sus scrofa) red deer (Cervus elaphus), roe deer (Capraelus capraelus), fallow deer (Dama dama), Spanish ibex (Capra pyrenaica hispanica) and mouflon (Ovis musimon), in Spain to assess the seroprevalence against Mycobacterium bovis or cross-reacting members of the Mycobcaterium tuberculosis complex (MTBC), and to provide information on associated risk factors...
2012: PloS One
https://www.readbyqxmd.com/read/22071126/performance-of-immunochromatographic-and-elisa-tests-for-detecting-fallow-deer-infected-with-mycobacterium-bovis
#12
M Boadella, J A Barasona, S Diaz-Sanchez, K P Lyashchenko, R Greenwald, J Esfandiari, C Gortazar
Fallow deer (Dama dama) are widely distributed as natural or naturalised populations, as well as in game parks and deer farms. We used 157 fallow deer sampled in populations considered to be Mycobacterium tuberculosis complex (MTC) free and 73 Mycobacterium bovis-infected fallow deer confirmed postmortem by culture to evaluate the diagnostic performance of two tests for the detection of anti-mycobacterial antibodies: the dual path platform (DPP) VetTB assay and the bovine purified protein derivative (bPPD) ELISA...
April 1, 2012: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/21581533/diaqua-n-n-bis-2-pyridylmethyl-ene-propane-1-3-diamine-manganese-ii-dibromide-aqua-bromido-n-n-bis-2-pyridylmethyl-ene-propane-1-3-diamine-manganese-ii-bromide-dibromido-n-n-bis-2-pyridylmethyl-ene-propane-1-3-diamine-manganese-ii-1-2-1
#13
In-Chul Hwang, Kwang Ha
There are three different Mn(II) complexes in the asymmetric unit of the title compound, [Mn(C(15)H(16)N(4))(H(2)O)(2)]Br(2)·2{[MnBr(C(15)H(16)N(4))(H(2)O)]Br}·[MnBr(2)(C(15)H(16)N(4))]. In the neutral complex, the Mn(2+) ion is six-coordinated in a distorted octa-hedral environment by four N atoms of the tetra-dentate ligand N,N'-bis-(2-pyridylmethyl-ene)propane-1,3-diamine (bppd) and two bromide ligands. In the two cationic complexes, the Mn(2+) ions are also six-coordinated in similar environments, but one Mn ion is coordinated by four N atoms of bppd, one Br atom and one O atom of a coordinating water mol-ecule, whereas the other Mn ion is coordinated by four N atoms of bppd and two O atoms of water ligands...
2008: Acta Crystallographica. Section E, Structure Reports Online
https://www.readbyqxmd.com/read/21217031/serologic-tests-for-detecting-antibodies-against-mycobacterium-bovis-and-mycobacterium-avium-subspecies-paratuberculosis-in-eurasian-wild-boar-sus-scrofa-scrofa
#14
Mariana Boadella, Konstantin Lyashchenko, Reena Greenwald, Javan Esfandiari, Raquel Jaroso, Tania Carta, Joseba M Garrido, Joaquín Vicente, José de la Fuente, Christian Gortázar
New tools to detect exposure of free-range Eurasian wild boar (Sus scrofa scrofa) to pathogenic mycobacteria would be valuable for improved disease surveillance and wildlife management. Two hundred sera from wild boar of known Mycobacterium bovis infection status were used to evaluate test suitability for the detection of antibodies against M. bovis and Mycobacterium avium subsp. paratuberculosis (or cross-reacting members of the M. avium complex). Two traditional enzyme-linked immunosorbent assays were evaluated using M...
January 2011: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/20807928/comparison-of-three-immunodiagnostic-assays-for-antemortem-detection-of-mycobacterium-bovis-stimulation-in-domestic-cats
#15
Karla A Fenton, Scott D Fitzgerald, John B Kaneene, John M Kruger, Rena Greenwald, Konstantin P Lyashchenko
Mycobacterium bovis causes disease in numerous mammalian species including humans, thus making research, surveillance, and control important in the eradication of tuberculosis. Domestic cats are susceptible to multiple mycobacterial species including Mycobacterium bovis; however, their role in the epidemiology of bovine tuberculosis is not fully documented. The current study was an evaluation of the immune response in specific pathogen-free (SPF) cats stimulated with sensitinogen, a heat-killed M. bovis product, using the rapid test, multiple antigen print immunoassay (MAPIA), and bovine-purified protein derivative (bPPD) single skin test...
September 2010: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/20633938/eurasian-wild-boar-response-to-skin-testing-with-mycobacterial-and-non-mycobacterial-antigens
#16
R Jaroso, J Vicente, I G Fernandez-de-Mera, A Aranaz, C Gortazar
Eurasian wild boar (Sus scrofa) are able to maintain bovine tuberculosis (bTB) in the wild and are most probably able to transmit the disease to other species, thus acting as a true wildlife reservoir. Translocation of wild boar is a common practice in European countries. Therefore, identifying effective tools for bTB diagnosis in living wild boar is crucial for the implementation of control measures. We describe for the first time the sex and origin related differences in the skin-test response to mycobacterial antigens (bPPD and aPPD) and to a non-mycobacterial antigen (PHA, a plant derived mitogen) in wild and farmed wild boar, and used a small sample of known M...
September 1, 2010: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/19423178/factors-affecting-red-deer-skin-test-responsiveness-to-bovine-and-avian-tuberculin-and-to-phytohaemagglutinin
#17
I G Fernández-de-Mera, J Vicente, U Höfle, F Ruiz Fons, J A Ortiz, C Gortázar
We studied the effect of management on the responsiveness of red deer (Cervus elaphus) to skin testing with mycobacterial and non-mycobacterial antigens. We hypothesized that individuals from populations of the same species under different management conditions would have a different immune responsiveness. Deer sampled in this study included 1041 adult animals from 6 Spanish farms and 111 adult wild deer. We injected four sites of the neck with 0.1 ml bovine purified protein derivative (PPD), 0.1 ml avian PPD, 0...
July 1, 2009: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/15708727/rules-for-the-classification-of-younger-children-with-nonverbal-learning-disabilities-and-basic-phonological-processing-disabilities
#18
Christine R Drummond, Saadia A Ahmad, Byron P Rourke
Rules for the classification of Nonverbal Learning Disabilities (NLD) and Basic Phonological Processing Disabilities (BPPD) that had been generated and tested on older children (ages 9-15) were applied to younger children (ages 7-8). The goal was to evaluate the applicability of these classification rules for a younger population with NLD and BPPD, and to make revisions if necessary. These rules were used to differentiate these two subtypes of learning disabilities using levels and patterns of performance on motor/psychomotor, tactile/perceptual, visual-spatial, auditory-perceptual, problem solving, and language measures...
March 2005: Archives of Clinical Neuropsychology: the Official Journal of the National Academy of Neuropsychologists
https://www.readbyqxmd.com/read/11935416/classification-rules-for-basic-phonological-processing-disabilities-and-nonverbal-learning-disabilities-formulation-and-external-validity
#19
P M Pelletier, S A Ahmad, B P Rourke
Rules for the classification of two subtypes of learning disabilities (LD), namely, Basic Phonological Processing Disabilities (BPPD) and Nonverbal Learning Disabilities (NLD), are reported. These rules (refined as a result of application to two separate samples of children with LD) were used to select children from an entirely new sample who could be classified as having "definite" or "probable" BPPD or NLD. As a first step in the external validation of these rules, the patterns of psychosocial functioning exhibited by these two subtypes of LD were determined...
June 2001: Child Neuropsychology: a Journal on Normal and Abnormal Development in Childhood and Adolescence
https://www.readbyqxmd.com/read/9150399/assessing-the-reliability-of-rna-folding-using-statistical-mechanics
#20
M Huynen, R Gutell, D Konings
We have analyzed the base-pairing probability distributions of 16 S and 16 S-like, and 23 S and 23 S-like ribosomal RNAs of Archaea, Bacteria, chloroplasts, mitochondria and Eukarya, as predicted by the partition function approach for RNA folding introduced by McCaskill. A quantitative analysis of the reliability of RNA folding is done by comparing the base-pairing probability distributions with the structures predicted by comparative sequence analysis (comparative structures). We distinguish two factors that show a relationship to the reliability of RNA minimum free energy structure...
April 18, 1997: Journal of Molecular Biology
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