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https://www.readbyqxmd.com/read/28944418/endogenous-epitope-tagging-of-heat-shock-protein-70-isoform-hsc70-using-crispr-cas9
#1
Nitika, Andrew W Truman
Heat shock protein 70 (Hsp70) is an evolutionarily well-conserved molecular chaperone involved in several cellular processes such as folding of proteins, modulating protein-protein interactions, and transport of proteins across the membrane. Binding partners of Hsp70 (known as "clients") are identified on an individual basis as researchers discover their particular protein of interest binds to Hsp70. A full complement of Hsp70 interactors under multiple stress conditions remains to be determined. A promising approach to characterizing the Hsp70 "interactome" is the use of protein epitope tagging and then affinity purification followed by mass spectrometry (AP-MS/MS)...
September 24, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28944359/crestal-sinus-lift-using-an-implant-with-an-internal-l-shaped-channel-1-year-after-loading-results-from-a-prospective-cohort-study
#2
Marco Tallarico, David L Cochran, Erta Xhanari, Claudia Dellavia, Elena Canciani, Eitan Mijiritsky, Silvio Mario Meloni
PURPOSE: To evaluate the clinical and radiographic outcomes of a one-stage crestal sinus elevation procedure using a self-tapping endosseous implant system (iRaise, Maxillent, Herzliya, Israel) developed for sinus augmentation, 1 year after loading. MATERIALS AND METHODS: Patients needing restoration in the posterior maxilla with a residual alveolar crest of 3 to 8 mm in height and 5 mm in width distal to the canine as measured on CBCT scan were treated using the iRaise sinus lift system...
2017: European Journal of Oral Implantology
https://www.readbyqxmd.com/read/28943728/short-peptide-epitope-design-from-hantaviruses-causing-hfrs
#3
Sathish Sankar, Mageshbabu Ramamurthy, Balaji Nandagopal, Gopalan Sridharan
Several genotypes of the hantavirus cause hemorrhagic fever with renal syndrome (HFRS) and is an important public health problem worldwide. There is now growing interest to develop subunit vaccines especially focused to elicit cytotoxic T lymphocyte responses which are important against viral infection. We identified candidate T-cell epitopes that bind to Class I HLA supertypes towards identifying potential subunit vaccine entity. These epitopes are conserved in all 5 hantavirus genotypes of HFRS (Hantaan, Dobrava- Belgrade, Seoul, Gou virus and Amur)...
2017: Bioinformation
https://www.readbyqxmd.com/read/28942319/highly-efficient-detection-of-salbutamol-in-environmental-water-samples-by-an-enzyme-immunoassay
#4
Zhenjiang Liu, Bo Zhang, Jianfan Sun, Yinhui Yi, Ming Li, Daolin Du, Fang Zhu, Jiawen Luan
Salbutamol (SAL) has potential hazardous effects on health, and has been found in natural water globally. To protect consumers from risks related to SAL residues, a fast and highly sensitive enzyme-linked immunosorbent assay (ELISA) was developed for the detection of SAL in environmental water samples. SAL hapten was synthesized and conjugated to proteins to be used as the immunogen for producing polyclonal antibodies. Under optimized conditions, the ELISA showed a 50% inhibitory concentration (IC50 value) of 0...
September 20, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28942244/kinetic-product-and-computational-studies-of-the-ultrasonic-induced-degradation-of-4-methylcyclohexanemethanol-mchm
#5
Danni Cui, Alexander M Mebel, Luis E Arroyo-Mora, Howard Holness, Kenneth G Furton, Kevin O'Shea
A massive spill of 4-methylcyclohexanemethanol (MCHM), a semi-volatile organic compound, contaminated the Elk river and forced the recent closure of tap water for nearly 300,000 residents. Typical water treatment methods are not effective for MCHM remediation, however ultrasonic irradiation leads to its rapid pseudo-first order degradation. The degradation processes were effectively modeled employing heterogeneous kinetic models with the reaction surface corresponding to the gas-liquid interface of the cavitation bubble...
September 5, 2017: Water Research
https://www.readbyqxmd.com/read/28941549/the-corticostriatal-adenosine-a2a-receptor-controls-maintenance-and-retrieval-of-spatial%C3%A2-working-memory
#6
Zhihui Li, Xingjun Chen, Tao Wang, Ying Gao, Fei Li, Long Chen, Jin Xue, Yan He, Yan Li, Wei Guo, Wu Zheng, Liping Zhang, Fenfen Ye, Xiangpeng Ren, Yue Feng, Piu Chan, Jiang-Fan Chen
BACKGROUND: Working memory (WM) taps into multiple executive processes including encoding, maintenance, and retrieval of information, but the molecular and circuit modulation of these WM processes remains undefined due to the lack of methods to control G protein-coupled receptor signaling with temporal resolution of seconds. METHODS: By coupling optogenetic control of the adenosine A2A receptor (A2AR) signaling, the Cre-loxP-mediated focal A2AR knockdown with a delayed non-match-to-place (DNMTP) task, we investigated the effect of optogenetic activation and focal knockdown of A2ARs in the dorsomedial striatum (n = 8 to 14 per group) and medial prefrontal cortex (n = 16 to 22 per group) on distinct executive processes of spatial WM...
August 1, 2017: Biological Psychiatry
https://www.readbyqxmd.com/read/28940944/multivariate-optimization-of-dispersive-liquid-liquid-microextraction-for-the-determination-of-paclobutrazol-and-triflumizole-in-water-by-gc-ms
#7
Dotse Selali Chormey, Yeşim Karakuş, Sena Karayaka, Çağla Özsöyler, Abdürrezzak Emin Bozdoğan, Sezgin Bakırdere
A new analytical method based on dispersive liquid-liquid microextraction with gas chromatography and mass spectrometry has been optimized for the simultaneous determination of paclobutrazol and triflumizole in tap water and wastewater samples. A two-level, full-factorial design that allowed the study of main effects and factor interactions was applied to analyze the influence on microextraction process by chloroform, ethanol, potassium iodide and hand shaking. The extraction conditions selected were 200 μL of chloroform, 3...
September 23, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28940164/fabrication-of-nanoscale-pits-with-high-throughput-on-polymer-thin-film-using-afm-tip-based-dynamic-plowing-lithography
#8
Yang He, Yanquan Geng, Yongda Yan, Xichun Luo
We show that an atomic force microscope (AFM) tip-based dynamic plowing lithography (DPL) approach can be used to fabricate nanoscale pits with high throughput. The method relies on scratching with a relatively large speed over a sample surface in tapping mode, which is responsible for the separation distance of adjacent pits. Scratching tests are carried out on a poly(methyl methacrylate) (PMMA) thin film using a diamond-like carbon coating tip. Results show that 100 μm/s is the critical value of the scratching speed...
September 22, 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28939415/qualitative-differences-in-offline-improvement-of-procedural-memory-by-daytime-napping-and-overnight-sleep-an-fmri-study
#9
Sho K Sugawara, Takahiko Koike, Hiroaki Kawamichi, Kai Makita, Yuki H Hamano, Haruka K Takahashi, Eri Nakagawa, Norihiro Sadato
Daytime napping offers various benefits for healthy adults, including enhancement of motor skill learning. It remains controversial whether napping can provide the same enhancement as overnight sleep, and if so, whether the same neural underpinning is recruited. To investigate this issue, we conducted functional MRI during motor skill learning, before and after a short day-nap, in 13 participants, and compared them with a larger group (N=47) who were tested following regular overnight sleep. Training in a sequential finger-tapping task required participants to press a keyboard in the MRI scanner with their non-dominant left hand as quickly and accurately as possible...
September 19, 2017: Neuroscience Research
https://www.readbyqxmd.com/read/28937607/tip-pressure-on-semicircular-specimens-in-tapping-mode-atomic-force-microscopy-in-viscous-fluid-environments
#10
Hua-Ju Shih, Ching-Liang Dai, Po-Jen Shih
Tapping mode (TM) atomic force microscopy (AFM) in a liquid environment is widely used to measure the contours of biological specimens. The TM triggers the AFM probe approximately at the resonant frequencies and controls the tip such that it periodically touches the specimen along the scanning path. The AFM probe and its tip produce a hydrodynamic pressure on the probe itself and press the specimen. The tip to specimen size ratio is known to affect the measurement accuracy of AFM, however, few studies have focused on the hydrodynamic pressure caused by the effects of specimen size...
September 22, 2017: Sensors
https://www.readbyqxmd.com/read/28937144/ubiquitous-euphorbia-is-anything-but-euphoria-for-the-eye-a-reminder-to-get-any-area-of-contact-with-the-toxic-sap-under-the-tap
#11
https://www.readbyqxmd.com/read/28936338/photochemically-active-dna-intercalating-ruthenium-and-related-complexes-insights-by-combining-crystallography-and-transient-spectroscopy
#12
Christine J Cardin, John M Kelly, Susan J Quinn
Recent research on the study of the interaction of ruthenium polypyridyl compounds and defined sequence nucleic acids is reviewed. Particular emphasis is paid to complexes [Ru(LL)2(Int)](2+) containing potentially intercalating ligands (Int) such as dipyridophenazine (dppz), which are known to display light-switching or photo-oxidising behaviour, depending on the nature of the ancillary ligands. X-ray crystallography has made a key contribution to our understanding, and the first complete survey of structural results is presented...
July 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28935825/effect-of-tapering-after-a-period-of-high-volume-sprint-interval-training-on-running-performance-and-muscular-adaptations-in-moderately-trained-runners
#13
Casper Skovgaard, Nicki Winfield Almquist, Thue Kvorning, Peter Møller Christensen, Jens Bangsbo
The effect of tapering following a period of high-volume sprint interval training (SIT) and a basic volume of aerobic training on performance and muscle adaptations in moderately trained runners was examined. Eleven (8 males, 3 females) runners (maximum oxygen uptake (VO2-max): 56.8±2.9 mL·min(-1)·kg(-1); mean±SD) conducted high-volume SIT (HV; 20 SIT sessions; 8-12 x 30-s all-out) for 40 days followed by 18 days of tapering (TAP; 4 SIT sessions; 4 x 30-s all-out). Before and after HV as well as midway through and at the end of TAP, the subjects completed a 10-km running test and a repeated running test at 90% of vVO2-max to exhaustion (RRT)...
September 21, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28935585/developmental-exposure-to-low-concentrations-of-two-brominated-flame-retardants-bde-47-and-bde-99-causes-life-long-behavioral-alterations-in-zebrafish
#14
Lilah Glazer, Corinne N Wells, Meghan Drastal, Kathryn-Ann Odamah, Richard E Galat, Mamta Behl, Edward D Levin
BACKGROUND: Polybrominated diphenyl ethers (PBDEs) were widely used as flame retardants until the early 2000s, mainly in home furnishings and electronics. The persistence of PBDEs in the environment leads to continued ubiquitous exposure to low levels, with infants and children experiencing higher exposures than adults. Accumulating evidence suggest that low-level exposures during early life stages can affect brain development and lead to long-term behavioral impairments. We investigated the effects of zebrafish exposure to low doses of the two prominent PBDEs; 2,2',4,4',5,-Pentabromodiphenyl ether (BDE-99) and 2,2',4,4',-Tetrabromodiphenyl ether (BDE-47), during embryo-development on short- and long-term behavioral endpoints...
September 18, 2017: Neurotoxicology
https://www.readbyqxmd.com/read/28935108/discovery-of-new-glycosidases-from-metagenomic-libraries
#15
Zach Armstrong, Peter Rahfeld, Stephen G Withers
Microorganisms routinely perform complex enzymatic transformations of natural material; thus, their enzymes have the potential to tackle medical and industrial challenges. However, a vast number of microbes are recalcitrant to cultivation. Functional screening of environmental DNA allows us to tap into the seemingly boundless diversity of enzymes encoded by microbial populations. In this chapter, we describe methods for the isolation of environmental DNA, generation of metagenomic libraries, and the functional screening of these libraries for new glycosidases...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28934777/effectiveness-of-thermal-annular-procedures-in-treating-discogenic-low-back-pain
#16
Standiford Helm Ii, Thomas T Simopoulos, Milan Stojanovic, Salahadin Abdi, Mohamed Ahamed El Terany
BACKGROUND: Discogenic low back is a distinct clinic entity characterized by pain arising from a damaged disc. The diagnosis is clouded by the controversy surrounding discography. The treatment options are limited, with unsatisfactory results from both conservative treatment and surgery. Multiple interventional therapies have been developed to treat discogenic pain, but most have not yet been validated by high quality studies.The best studied treatment for discogenic pain is the use of heat, which has been labeled as thermal intradiscal procedures (TIPs) by the Centers for Medicare and Medicaid Services (CMS)...
September 2017: Pain Physician
https://www.readbyqxmd.com/read/28934722/rice-consumption-and-squamous-cell-carcinoma-of-the-skin-in-a-united-states-population
#17
Anala Gossai, M Scot Zens, Tracy Punshon, Brian P Jackson, Ann E Perry, Margaret R Karagas
BACKGROUND: Rice contains arsenic, a known skin carcinogen. Rice intake has been associated with arsenic-related skin lesions in South Asia, but its association with skin cancers is as yet unknown. OBJECTIVES: We aimed to investigate whether rice intake contributes to urinary arsenic concentration and risk of squamous cell carcinoma (SCC) of the skin in a U.S. population. METHODS: Rice consumption was assessed using a food frequency questionnaire administered as part of a population-based case-control study of 487 SCC cases and 462 age- and gender-matched controls...
September 7, 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28934099/phase-imaging-and-nanoscale-energy-dissipation-of-supported-graphene-using-amplitude-modulation-atomic-force-microscopy
#18
Borislav Vasic, Aleksandar Matkovic, Rados Gajic
We investigate phase imaging of supported graphene using amplitude modulation atomic force microscopy (AFM), so called, tapping mode. The phase contrast between graphene and neighbouring substrate grows at hard tapping conditions and the contrast is enhanced compared to the topographic one. Therefore, the phase measurements could enable a high contrast imaging of graphene and related two dimensional materials and heterostructures, not achievable with conventional AFM based topographic measurements. Obtained phase maps are then transformed into energy dissipation maps which are important for graphene applications in various mechanical systems...
September 21, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28933478/natural-product-modulators-of-human-sensations-and-mood-molecular-mechanisms-and-therapeutic-potential
#19
REVIEW
Tomáš Pluskal, Jing-Ke Weng
Humans perceive physical information about the surrounding environment through their senses. This physical information is registered by a collection of highly evolved and finely tuned molecular sensory receptors. A multitude of bioactive, structurally diverse ligands have evolved in nature that bind these molecular receptors. The complex, dynamic interactions between the ligands and the receptors lead to changes in our sensory perception or mood. Here, we review our current knowledge of natural products and their derived analogues that interact specifically with human G protein-coupled receptors, ion channels, and nuclear hormone receptors to modulate the sensations of taste, smell, temperature, pain, and itch, as well as mood and its associated behaviour...
September 21, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28932958/sensitive-determination-of-cadmium-using-solidified-floating-organic-drop-microextraction-slotted-quartz-tube-flame-atomic-absorption-spectroscopy
#20
Erhan Akkaya, Dotse Selali Chormey, Sezgin Bakırdere
In this study, solidified floating organic drop microextraction (SFODME) by 1-undecanol was combined with slotted quartz tube flame atomic absorption spectrometry (SQT-FAAS) for the determination of cadmium at trace levels. Formation of a complex with 4,4'-dimethyl-2,2'-bipyridine facilitated the extraction of cadmium from aqueous solutions. Several chemical variables were optimized in order to obtain high extraction outputs. Parameters such as concentration of the ligand, pH, and amount of buffer solution were optimized to enhance the formation of cadmium complex...
September 20, 2017: Environmental Monitoring and Assessment
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