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E Kaouech, K Kallel, S Anane, S Belhadj, S Abdellatif, K Mnif, T Ben Othmane, S Ben Lakhal, B Kilani, T Ben Châabane, E Chaker
Diagnosis of pneumocystis pneumonia is usually based on clinical features and X-rays photography and confirmed in the laboratory by visualisation of Pneumocystis organisms in stained preparations of respiratory specimens using several techniques (Gomori-Grocott, May-Grünwald Giemsa, bleu de toluidine O). Actually, PCR has considerably increased sensitivity of detection of Pneumocystis. The aim of this study is to compare conventional PCR results to those of staining techniques (Gomori-Grocott, May-Grünwald Giemsa) in addition to the X-ray and clinical findings in order to evaluate the contribution of each method...
July 2009: Pathologie-biologie
Christine Demanche, Fanelie Wanert, Nicolas Herrenschmidt, Claire Moussu, Isabelle Durand-Joly, Eduardo Dei-Cas, Rene Chermette, Jacques Guillot
As monkeys-derived Pneumocystis is closely related to P. jirovecii, simian populations should be considered as valuable models for the understanding of the epidemiology of human pneumocystosis. In the present study, the impact of environmental factors on the carriage of Pneumocystis was evaluated in socially organized group of immunocompetent macaques (Macaca fascicularis). The tribe, maintained in partial release at the Primatology Center of Strasbourg in France, comprised 29 animals at the end of the study...
2003: Journal of Eukaryotic Microbiology
M A Khan, N Farrag, P Butcher
OBJECTIVES: to compare immunofluorescence (IF) test, routinely used in the department for the detection of Pnemocystis carinni with simple polymerase chain reaction (PCR) and nested PCR (nPCR) METHODS: Bronchoalveolar lavage (BAL) and induced sputum (IS) specimens from HIV-positive (39), lung transplant ssart transplant (2), and one each from non-Hodgkin's lymphoma, drug addict and a premature baby were screened by IF test, simple PCR and nPCR for the presence of P.carinii. RESULTS: of the 46 specimens tested, two (4...
July 1999: Journal of Infection
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