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https://www.readbyqxmd.com/read/28105837/hrp-mimicking-dnazyme-catalyzed-in-situ-generation-of-polyaniline-to-assist-signal-amplification-for-ultrasensitive-surface-plasmon-resonance-biosensing
#1
Haiyin Li, Jiafu Chang, Ting Hou, Feng Li
It is well-known that the horseradish peroxidase- (HRP-) mimicking DNAzyme, namely, hemin/G-quadruplex, can effectively catalyze the polymerization of aniline to form DNA-guided polyaniline. Meanwhile, polyaniline exhibits extraordinary electrical, electrochemical, and redox properties, as well as excellent SPR signal-enhancing ability. Herein, we report a novel ultrasensitive surface plasmon resonance (SPR) biosensor based on HRP-mimicking DNAzyme-catalyzed in situ formation of polyaniline for signal amplification, using bleomycin (BLM) as the proof-of-concept analyte...
January 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28066798/an-ngago-tool-for-genome-editing-did-crispr-cas9-just-find-a-competitor
#2
Qiang Wei, Junyi Liao, Xinyi Yu, Eric J Wang, Claire Wang, Hue H Luu, Rex C Haydon, Michael J Lee, Tong-Chuan He
While CRISPR/Cas9-mediated genome editing technology has been experiencing a rapid transformation during the past few years, a recent report on NgAgo-mediated single-stranded DNA-guided genome editing may offer an attractive alternative for genome manipulation. While it's too early to predict whether NgAgo will be able to compete with or be superior to CRISPR/Cas9, the scientific community is anxiously waiting for further optimization and broader applications of the NgAgo genome editing technology.
September 2016: Genes & Diseases
https://www.readbyqxmd.com/read/28053232/directional-emission-from-dye-functionalized-plasmonic-dna-superlattice-microcavities
#3
Daniel J Park, Jessie C Ku, Lin Sun, Clotilde M Lethiec, Nathaniel P Stern, George C Schatz, Chad A Mirkin
Three-dimensional plasmonic superlattice microcavities, made from programmable atom equivalents comprising gold nanoparticles functionalized with DNA, are used as a testbed to study directional light emission. DNA-guided nanoparticle colloidal crystallization allows for the formation of micrometer-scale single-crystal body-centered cubic gold nanoparticle superlattices, with dye molecules coupled to the DNA strands that link the particles together, in the form of a rhombic dodecahedron. Encapsulation in silica allows one to create robust architectures with the plasmonically active particles and dye molecules fixed in space...
January 17, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27893702/failure-to-detect-dna-guided-genome-editing-using-natronobacterium-gregoryi-argonaute
#4
Seung Hwan Lee, Giandomenico Turchiano, Hirotaka Ata, Somaira Nowsheen, Marianna Romito, Zhenkun Lou, Seuk-Min Ryu, Stephen C Ekker, Toni Cathomen, Jin-Soo Kim
No abstract text is available yet for this article.
November 28, 2016: Nature Biotechnology
https://www.readbyqxmd.com/read/27813013/dna-directed-self-assembly-of-highly-ordered-and-dense-single-walled-carbon-nanotube-arrays
#5
Hareem Maune, Si-Ping Han
Single-walled carbon nanotubes (SWNT or CNT) have unique and well-known high-performance material properties that can enable revolutionary increases in the performance of electronic devices and architectures. However, fabrication of large-scale SWNT-based ICs is an enormously challenging, unsolved problem, and self-assembly is likely needed for critical steps. Over the past several years, methods have been introduced to created ordered carbon nanotube structures using DNA guided self-assembly. In this chapter, we briefly review the challenges involved in using DNA to assemble SWNT nanostructures, and then give detailed methods to assemble dense, aligned SWNT arrays...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27741323/mechanistic-insights-into-archaeal-and-human-argonaute-substrate-binding-and-cleavage-properties
#6
Sarah Willkomm, Adrian Zander, Dina Grohmann, Tobias Restle
Argonaute (Ago) proteins from all three domains of life are key players in processes that specifically regulate cellular nucleic acid levels. Some of these Ago proteins, among them human Argonaute2 (hAgo2) and Ago from the archaeal organism Methanocaldococcus jannaschii (MjAgo), are able to cleave nucleic acid target strands that are recognised via an Ago-associated complementary guide strand. Here we present an in-depth kinetic side-by-side analysis of hAgo2 and MjAgo guide and target substrate binding as well as target strand cleavage, which enabled us to disclose similarities and differences in the mechanistic pathways as a function of the chemical nature of the substrate...
2016: PloS One
https://www.readbyqxmd.com/read/27730722/dry-sintering-meets-wet-silver-ion-soldering-charge-transfer-plasmon-engineering-of-solution-assembled-gold-nanodimers-from-visible-to-near-infrared%C3%A2-i-and-ii%C3%A2-regions
#7
Lingling Fang, Yueliang Wang, Miao Liu, Ming Gong, An Xu, Zhaoxiang Deng
Achieving highly tunable and localized surface plasmon resonance up to near infrared (NIR) regions is a key target in nanoplasmonics. In particular, a self-assembly process capable of producing highly uniform and solution-processable nanomaterials with tailor-made plasmonic properties is lacking. We herein address this problem through a conjunctive use of wet Ag(+) soldering and dry thermal sintering to produce nanodimer-derived structures with precisely engineered charge-transfer plasmon (CTP). The sintered dimers are water soluble, featuring gradually shifted CTP spanning an 800 nm wavelength range (up to NIR II)...
October 12, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27640875/dna-guided-genome-editing-using-structure-guided-endonucleases
#8
Gaurav K Varshney, Shawn M Burgess
The search for novel ways to target and alter the genomes of living organisms accelerated rapidly this decade with the discovery of CRISPR/Cas9. Since the initial discovery, efforts to find alternative methods for altering the genome have expanded. A new study presenting an alternative approach has been demonstrated that utilizes flap endonuclease 1 (FEN-1) fused to the Fok1 endonuclease, which shows potential for DNA-guided genome targeting in vivo.
2016: Genome Biology
https://www.readbyqxmd.com/read/27571968/crispr-cas9-a-promising-way-to-exploit%C3%A2-genetic-variation-in-plants
#9
REVIEW
Reema Rani, Prashant Yadav, Kalyani M Barbadikar, Nikita Baliyan, Era Vaidya Malhotra, Binay Kumar Singh, Arun Kumar, Dhiraj Singh
Creation of variation in existing gene pool of crop plants is the foremost requirement in crop improvement programmes. Genome editing is a tool to produce knock out of target genes either by introduction of insertion or by deletion that disrupts the function of a specific gene. The CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) system is the most recent addition to the toolbox of sequence-specific nucleases that includes ZFNs and TALENs. The CRISPR/Cas9 system allows targeted cleavage of genomic DNA guided by a small noncoding RNA, resulting in gene modifications by both non-homologous end joining and homology-directed repair mechanisms...
December 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27514463/laboratory-medicine-in-the-clinical-decision-support-for-treatment-of-hypercholesterolemia-pharmacogenetics-of-statins
#10
REVIEW
Gualberto Ruaño, Richard Seip, Andreas Windemuth, Alan H B Wu, Paul D Thompson
Statin responsiveness is an area of great research interest given the success of the drug class in the treatment of hypercholesterolemia and in primary and secondary prevention of cardiovascular disease. Interrogation of the patient's genome for gene variants will eventually guide anti-hyperlipidemic intervention. In this review, we discuss methodological approaches to discover genetic markers predictive of class-wide and drug-specific statin efficacy and safety. Notable pharmacogenetic findings are summarized from hypothesis-free genome wide and hypothesis-led candidate gene association studies...
September 2016: Clinics in Laboratory Medicine
https://www.readbyqxmd.com/read/27428505/dna-scaffolds-for-the-dictated-assembly-of-left-right-handed-plasmonic-au-np-helices-with-programmed-chiro-optical-properties
#11
Alessandro Cecconello, Jason S Kahn, Chun-Hua Lu, Larousse Khosravi Khorashad, Alexander O Govorov, Itamar Willner
Within the broad interest of assembling chiral left- and right-handed helices of plasmonic nanoparticles (NPs), we introduce the DNA-guided organization of left- or right-handed plasmonic Au NPs on DNA scaffolds. The method involves the self-assembly of stacked 12 DNA quasi-rings interlinked by 30 staple-strands. By the functionalization of one group of staple units with programmed tether-nucleic acid strands and additional staple elements with long nucleic acid chains, acting as promoter strands, the promoter-guided assembly of barrels modified with 12 left- or right-handed tethers is achieved...
August 10, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27207137/dna-guided-genome-editing-tool
#12
Dandan Zhang, Jian-Feng Li
No abstract text is available yet for this article.
July 2016: Science China. Life Sciences
https://www.readbyqxmd.com/read/27136078/dna-guided-genome-editing-using-the-natronobacterium-gregoryi-argonaute
#13
Feng Gao, Xiao Z Shen, Feng Jiang, Yongqiang Wu, Chunyu Han
The RNA-guided endonuclease Cas9 has made genome editing a widely accessible technique. Similar to Cas9, endonucleases from the Argonaute protein family also use oligonucleotides as guides to degrade invasive genomes. Here we report that the Natronobacterium gregoryi Argonaute (NgAgo) is a DNA-guided endonuclease suitable for genome editing in human cells. NgAgo binds 5' phosphorylated single-stranded guide DNA (gDNA) of ∼24 nucleotides, efficiently creates site-specific DNA double-strand breaks when loaded with the gDNA...
July 2016: Nature Biotechnology
https://www.readbyqxmd.com/read/26990992/structural-plasticity-of-pam-recognition-by-engineered-variants-of-the-rna-guided-endonuclease-cas9
#14
Carolin Anders, Katja Bargsten, Martin Jinek
The RNA-guided endonuclease Cas9 from Streptococcus pyogenes (SpCas9) forms the core of a powerful genome editing technology. DNA cleavage by SpCas9 is dependent on the presence of a 5'-NGG-3' protospacer adjacent motif (PAM) in the target DNA, restricting the choice of targetable sequences. To address this limitation, artificial SpCas9 variants with altered PAM specificities have recently been developed. Here we report crystal structures of the VQR, EQR, and VRER SpCas9 variants bound to target DNAs containing their preferred PAM sequences...
March 17, 2016: Molecular Cell
https://www.readbyqxmd.com/read/26851184/dna-guided-precision-medicine-for-cancer-a-case-of-irrational-exuberance
#15
Emile E Voest, Rene Bernards
Precision treatment with targeted cancer drugs requires the selection of patients who are most likely to benefit from a given therapy. We argue here that the use of a combination of both DNA and transcriptome analyses will significantly improve drug response prediction.
February 2016: Cancer Discovery
https://www.readbyqxmd.com/read/26725969/bio-recognitive-photonics-of-a-dna-guided-organic-semiconductor
#16
Seung Hyuk Back, Jin Hyuk Park, Chunzhi Cui, Dong June Ahn
Incorporation of duplex DNA with higher molecular weights has attracted attention for a new opportunity towards a better organic light-emitting diode (OLED) capability. However, biological recognition by OLED materials is yet to be addressed. In this study, specific oligomeric DNA-DNA recognition is successfully achieved by tri (8-hydroxyquinoline) aluminium (Alq3), an organic semiconductor. Alq3 rods crystallized with guidance from single-strand DNA molecules show, strikingly, a unique distribution of the DNA molecules with a shape of an 'inverted' hourglass...
2016: Nature Communications
https://www.readbyqxmd.com/read/26492515/mechanistic-insight-into-dna-guided-control-of-nanoparticle-morphologies
#17
Li Huey Tan, Yuan Yue, Nitya Sai Reddy Satyavolu, Arzeena Sultana Ali, Zidong Wang, Yuqing Wu, Yi Lu
Although shapes and surface characteristics of nanoparticles are known to play important roles in defining their properties, it remains challenging to fine-tune the morphologies systematically and predictably. Recently, we have shown that DNA molecules can serve as programmable ligands to fine-tune the morphologies of nanomaterials. Despite this discovery, the mechanism of how the morphology can be controlled and the roles of the DNA molecules in contributing to such control are not understood. We herein report mechanistic investigation of DNA-mediated morphological evolution of gold nanoprism seeds into nonagon, hexagon, and six-pointed stars, some of which display rough surfaces, in the presence of homo-oligomeric T30, G20, C30, and A30...
November 18, 2015: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/26330564/dna-guided-establishment-of-nucleosome-patterns-within-coding-regions-of-a-eukaryotic-genome
#18
Leslie Y Beh, Manuel M Müller, Tom W Muir, Noam Kaplan, Laura F Landweber
A conserved hallmark of eukaryotic chromatin architecture is the distinctive array of well-positioned nucleosomes downstream from transcription start sites (TSS). Recent studies indicate that trans-acting factors establish this stereotypical array. Here, we present the first genome-wide in vitro and in vivo nucleosome maps for the ciliate Tetrahymena thermophila. In contrast with previous studies in yeast, we find that the stereotypical nucleosome array is preserved in the in vitro reconstituted map, which is governed only by the DNA sequence preferences of nucleosomes...
November 2015: Genome Research
https://www.readbyqxmd.com/read/26241189/modular-optimization-of-multi-gene-pathways-for-fumarate-production
#19
Xiulai Chen, Pan Zhu, Liming Liu
Microbial fumarate production from renewable feedstock is a promising and sustainable alternative to petroleum-based chemical synthesis. Here, we report a modular engineering approach that systematically removed metabolic pathway bottlenecks and led to significant titer improvements in a multi-gene fumarate metabolic pathway. On the basis of central pathway architecture, yeast fumarate biosynthesis was re-cast into three modules: reduction module, oxidation module, and byproduct module. We targeted reduction module and oxidation module to the cytoplasm and the mitochondria, respectively...
January 2016: Metabolic Engineering
https://www.readbyqxmd.com/read/26140366/approaching-the-golden-fleece-a-molecule-at-a-time-biophysical-insights-into-argonaute-instructed-nucleic-acid-interactions
#20
REVIEW
Veronika A Herzog, Stefan L Ameres
Argonaute proteins act at the core of nucleic acid-guided interference pathways that regulate gene expression and defend organisms against foreign genetic elements in all domains of life. Here, we review recent biophysical studies on how Argonaute proteins instruct oligonucleotides in the process of target finding, binding, cleavage, and release, as measured at high spatiotemporal resolution by single-molecule approaches. In the context of previous structural, biochemical, and computational studies, a model emerges for how Argonaute proteins manipulate the thermodynamic rules for nucleic acid hybridization to convey efficiency and specificity to RNA- and DNA-guided regulatory processes...
July 2, 2015: Molecular Cell
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