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https://www.readbyqxmd.com/read/29668071/human-papillomavirus-an-unlikely-etiologic-factor-in-sinonasal-inverted-papilloma
#1
Sepideh Mohajeri, Chi Lai, Bibianna Purgina, Dakheelallah Almutairi, Tabassom Baghai, Jim Dimitroulakos, Shaun Kilty
OBJECTIVES/HYPOTHESIS: Tenuous evidence has supported the hypothesis that sinonasal inverted papilloma (SNIP) arise from human papillomavirus (HPV) infection. To clarify the role of HPV in SNIP, all known HPV sub-types were evaluated by employing a robust polymerase chain reaction-based method in a wide variety of SNIPs from a single institution. STUDY DESIGN: Retrospective surgical specimen tumor sample analysis. METHODS: HPV positivity among SNIP samples and those with squamous cell carcinoma (SCC) were compared...
April 18, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29667857/determining-risk-factors-that-increase-hospitalizations-in-patients-with-systemic-lupus-erythematosus
#2
D Li, H M Madhoun, W N Roberts, W Jarjour
Introduction Systemic lupus erythematosus (SLE) is a complex disease that is associated with significant mortality and an increased risk of hospitalization. Several validated instruments are available to measure disease activity in SLE patients. However, these instruments were not designed to screen for SLE patients at an increased risk of hospitalization. These instruments also fail to incorporate some data that are easily obtainable from electronic health records, such as the frequency of missed outpatient appointments...
January 1, 2018: Lupus
https://www.readbyqxmd.com/read/29667782/hypofolins-a-l-ent-labdane-diterpenoids-from-the-roots-of-hypoestes-phyllostachya-pink-splash
#3
Bin Cheng, Lin-Fen Ding, Tong Yan, Zhang-Qiao Xie, Zhi-Jun Zhang, Liu-Dong Song, Xing-De Wu, Qin-Shi Zhao
Twelve new ent-labdane diterpenoids, hypofolins A-F (1-6) and hypofolins G-L (7a/7b, 8a/8b, and 9a/9b), were isolated from the roots of Hypoestes phyllostachya "Pink Splash". Their structures were elucidated by extensive 1D and 2D NMR spectroscopic and HRMS data. The absolute configurations of 1, 2, 5, and 7a/7b were determined by single crystal X-ray diffraction and ECD analysis, as well as chemical transformations. Compounds 7a/7b, 8a/8b, and 9a/9b were isolated as three pairs of interconverting mixture of two isomers between ketone and hemiketal types...
April 18, 2018: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/29667293/enhanced-in-vitro-biocompatibility-and-water-dispersibility-of-magnetite-and-cobalt-ferrite-nanoparticles-employed-as-ros-formation-enhancer-in-radiation-cancer-therapy
#4
Stefanie Klein, Melek Kızaloğlu, Luis Portilla, Hyoungwon Park, Tobias Rejek, Julian Hümmer, Karsten Meyer, Rainer Hock, Luitpold V R Distel, Marcus Halik, Carola Kryschi
Efficient magnetic reactive oxygen species (ROS) formation enhancing agents after X-ray treatment are realized by functionalizing superparamagnetic magnetite (Fe3 O4 ) and Co-ferrite (CoFe2 O4 ) nanoparticles with self-assembled monolayers (SAMs). The Fe3 O4 and CoFe2 O4 nanoparticles are synthesized using Massart's coprecipitation technique. Successful surface modification with the SAM forming compounds 1-methyl-3-(dodecylphosphonic acid) imidazolium bromide, or (2-{2-[2-hydroxy-ethoxy]-ethoxy}-ethyl phosphonic acid provides biocompatibility and long-term stability of the Fe3 O4 and CoFe2 O4 nanoparticles in cell media...
April 18, 2018: Small
https://www.readbyqxmd.com/read/29667135/optimising-intratumoral-treatment-of-head-and-neck-squamous-cell-carcinoma-models-with-the-diterpene-ester-tigilanol-tiglate
#5
Catherine M E Barnett, Natasa Broit, Pei-Yi Yap, Jason K Cullen, Peter G Parsons, Benedict J Panizza, Glen M Boyle
The five-year survival rate for patients with head and neck squamous cell carcinoma (HNSCC) has remained at ~50% for the past 30 years despite advances in treatment. Tigilanol tiglate (TT, also known as EBC-46) is a novel diterpene ester that induces cell death in HNSCC in vitro and in mouse models, and has recently completed Phase I human clinical trials. The aim of this study was to optimise efficacy of TT treatment by altering different administration parameters. The tongue SCC cell line (SCC-15) was identified as the line with the lowest efficacy to treatment...
April 18, 2018: Investigational New Drugs
https://www.readbyqxmd.com/read/29667009/encapsulating-viability-of-multi-strain-lactobacilli-as-potential-probiotic-in-pigs
#6
Sudthidol Piyadeatsoontorn, Rutjawate Taharnklaew, Tewa Upathanpreecha, Pairat Sornplang
Important aspects of the selection of probiotics to be used for mixing in animal feed include host species specificity and probiotic cell survival during production and storage of their products. The research was to screen and investigate some probiotic properties of lactic acid bacteria (LAB) isolated from pig fecal samples. One hundred and thirty-eight representative LAB isolates, which were isolated from 51 pig fecal samples, were tested for acid and bile tolerance, antimicrobial susceptibility, antibacterial activity, potential adhesion to the cell surface, and survival rates when stored in varied microencapsulation forms: freeze-dried, spray-dried, and micro-beads...
April 17, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29666888/precise-determination-of-protein-extinction-coefficients-under-native-and-denaturing-conditions-using-sv-auc
#7
Andreas Hoffmann, Kerstin Grassl, Janine Gommert, Christian Schlesak, Alexander Bepperling
The accurate determination of protein concentration is an important though non-trivial task during the development of a biopharmaceutical. The fundamental prerequisite for this is the availability of an accurate extinction coefficient. Common approaches for the determination of an extinction coefficient for a given protein are either based on the theoretical prediction utilizing the amino acid sequence or the photometric determination combined with a measurement of absolute protein concentration. Here, we report on an improved SV-AUC based method utilizing an analytical ultracentrifuge equipped with absorbance and Rayleigh interference optics...
April 17, 2018: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/29666477/single-cell-analysis-of-pyroptosis-dynamics-reveals-conserved-gsdmd-mediated-subcellular-events-that-precede-plasma-membrane-rupture
#8
Nathalia M de Vasconcelos, Nina Van Opdenbosch, Hanne Van Gorp, Eef Parthoens, Mohamed Lamkanfi
Pyroptosis is rapidly emerging as a mechanism of anti-microbial host defense, and of extracellular release of the inflammasome-dependent cytokines interleukin (IL)-1β and IL-18, which contributes to autoinflammatory pathology. Caspases 1, 4, 5 and 11 trigger this regulated form of necrosis by cleaving the pyroptosis effector gasdermin D (GSDMD), causing its pore-forming amino-terminal domain to oligomerize and perforate the plasma membrane. However, the subcellular events that precede pyroptotic cell lysis are ill defined...
April 17, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29666434/stable-transformation-of-babesia-bigemina-and-babesia-bovis-using-a-single-transfection-plasmid
#9
Marta G Silva, Donald P Knowles, Monica L Mazuz, Brian M Cooke, Carlos E Suarez
Babesia bigemina and Babesia bovis, are the two major causes of bovine babesiosis, a global neglected disease in need of improved methods of control. Here, we describe a shared method for the stable transfection of these two parasites using electroporation and blasticidin/blasticidin deaminase as a selectable marker. Stably transfected B. bigemina and B. bovis were obtained using a common transfection plasmid targeting the enhanced green fluorescent protein-BSD (egfp-bsd) fusion gene into the elongation factor-1α (ef-1α) locus of B...
April 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29666189/single-cell-rna-seq-reveals-cell-heterogeneity-and-hierarchy-within-mouse-mammary-epithelia
#10
Heng Sun, Zhengqiang Miao, Xin Zhang, Un In Chan, Sek Man Su, Sen Guo, Chris Koon Ho Wong, Xiaoling Xu, Chu-Xia Deng
The mammary gland is very intricately and well organized into distinct tissues, including epithelia, endothelia, adipocytes, and stromal and immune cells. Many mammary gland diseases, such as breast cancer arise from abnormalities in the mammary epithelium, which is mainly composed of two distinct lineages, the basal and luminal cells. Because of the limitation of traditional transcriptome analysis of bulk mammary cells, the hierarchy and heterogeneity of mammary cells within these two lineages remain unclear...
April 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29666149/genetic-and-phenotypic-difference-in-cd8-t-cell-exhaustion-between-chronic-hepatitis-b-infection-and-hepatocellular-carcinoma
#11
Xiaochen Wang, Qifeng He, Haiyuan Shen, Xiao-Jie Lu, Beicheng Sun
BACKGROUND: Several recent studies published have suggested that T cell exhaustion exists both in chronic infection and cancer. However, to date, few studies have investigated their differences. Here we designed this study to explore the genetic and phenotypic difference in CD8+ T cell exhaustion between chronic hepatitis B (CHB) and hepatocellular carcinoma (HCC). METHODS: In this study, we assayed the phenotypes and functional states of CD8+ T cells separating from human CHB tissues and HCC tissues, and re-analyse the single-cell sequencing data (GSE98638) published previously...
April 17, 2018: Journal of Medical Genetics
https://www.readbyqxmd.com/read/29665662/-association-of-single-nucleotide-polymorphisms-of-tissue-factor-and-tissue-factor-pathway-inhibitor-with-venous-thromboembolism-in-patients-with-lung-cancer
#12
X L Zhang, J Zhu, W H Chen, Z G Zhai, Y H Zhang
Objective: s To investigate the distributions of single nucleotide polymorphisms (SNPs) of tissue factor (TF) and its inhibitor tissue factor pathway inhibitor (TFPI) and analyze the association of the SNPs with venous thromboembolism (VTE) in lung cancer patients. Methods: Between October 2009 and August 2013, a total of 152 hospitalized patients with newly diagnosed primary non-small cell lung cancer were enrolled from Beijing Chao Yang Hospital. Among them [male 105 cases, female 47 cases, with an age of (62...
March 27, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29665553/development-of-microfluidic-impedance-cytometry-enabling-the-quantification-of-specific-membrane-capacitance-and-cytoplasm-conductivity-from-100-000-single-cells
#13
Yang Zhao, Ke Wang, Deyong Chen, Beiyuan Fan, Ying Xu, Yifei Ye, Junbo Wang, Jian Chen, Chengjun Huang
This paper presents a new microfluidic impedance cytometry with crossing constriction microchannels, enabling the characterization of cellular electrical markers (e.g., specific membrane capacitance (Csm ) and cytoplasm conductivity (σcy )) in large cell populations (~ 100,000 cells) at a rate greater than 100 cells/s. Single cells were aspirated continuously through the major constriction channel with a proper sealing of the side constriction channel. An equivalent circuit model was developed and the measured impedance values were translated to Csm and σcy ...
April 7, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29665400/tolerant-industrial-yeast-saccharomyces-cerevisiae-posses-a-more-robust-cell-wall-integrity-signaling-pathway-against-2-furaldehyde-and-5-hydroxymethyl-2-furaldehyde
#14
Z Lewis Liu, Xu Wang, Scott A Weber
Cell wall integrity signaling pathway in Saccharomyces cerevisiae is a conserved function for detecting and responding to cell stress conditions but less understood for industrial yeast. We examined gene expression dynamics for a tolerant industrial yeast strain NRRL Y-50049 in response to challenges of furfural and HMF through comparative quantitative gene expression analysis using pathway-based qRT-PCR array assays. All tested genes from Y-50049, except for MLP2, demonstrated more resistant and significantly increased gene expression than that from a laboratory strain BY4741...
April 14, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29665375/luteolin-escape-mutants-of-dengue-virus-map-to-prm-and-ns2b-and-reveal-viral-plasticity-during-maturation
#15
Minhua Peng, Crystall Marie Dawn Swarbrick, Kitti Wing-Ki Chan, Dahai Luo, Wei Zhang, Xiaoping Lai, Geng Li, Subhash G Vasudevan
We previously showed that luteolin, a well-known plant-derived component found in the "heat clearing" class of Traditional Chinese Medicine (TCM) herbs, is an uncompetitive inhibitor (Ki 58.6 μM) of the host proprotein convertase furin, an endoprotease that is required for maturation of flaviviruses in the trans-Golgi compartment. Luteolin also weakly inhibited recombinant dengue virus NS2B/NS3 protease (Ki 140.36 μM) non-competitively. In order to further explore the mechanism of inhibition we isolated resistant mutants by continuous passaging of DENV2 in the presence of increasing concentrations of luteolin...
April 14, 2018: Antiviral Research
https://www.readbyqxmd.com/read/29665274/combining-multiplex-sers-nanovectors-and-multivariate-analysis-for-in-situ-profiling-of-circulating-tumor-cell-phenotype-using-a-microfluidic-chip
#16
Yizhi Zhang, Zhuyuan Wang, Lei Wu, Shenfei Zong, Binfeng Yun, Yiping Cui
Isolating and in situ profiling the heterogeneous molecular phenotype of circulating tumor cells are of great significance for clinical cancer diagnosis and personalized therapy. Herein, an on-chip strategy is proposed that combines size-based microfluidic cell isolation with multiple spectrally orthogonal surface-enhanced Raman spectroscopy (SERS) analysis for in situ profiling of cell membrane proteins and identification of cancer subpopulations. With the developed microfluidic chip, tumor cells are sieved from blood on the basis of size discrepancy...
April 17, 2018: Small
https://www.readbyqxmd.com/read/29665244/dafi-a-directed-recursive-data-filtering-and-clustering-approach-for-improving-and-interpreting-data-clustering-identification-of-cell-populations-from-polychromatic-flow-cytometry-data
#17
Alexandra J Lee, Ivan Chang, Julie G Burel, Cecilia S Lindestam Arlehamn, Aishwarya Mandava, Daniela Weiskopf, Bjoern Peters, Alessandro Sette, Richard H Scheuermann, Yu Qian
Computational methods for identification of cell populations from polychromatic flow cytometry data are changing the paradigm of cytometry bioinformatics. Data clustering is the most common computational approach to unsupervised identification of cell populations from multidimensional cytometry data. However, interpretation of the identified data clusters is labor-intensive. Certain types of user-defined cell populations are also difficult to identify by fully automated data clustering analysis. Both are roadblocks before a cytometry lab can adopt the data clustering approach for cell population identification in routine use...
April 17, 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29664094/analysis-of-poly-adp-ribose-polymerase-1-by-enzyme-initiated-auto-parylation-controlled-aggregation-of-hemin-graphene-nanocomposites
#18
Yong Liu, Xiaolin Xu, Haitang Yang, Ensheng Xu, Shuangshuang Wu, Wei Wei, Jin Chen
Poly(ADP-ribose) polymerase-1 (PARP-1) is a highly conserved nuclear enzyme, which binds tightly to damaged DNA and plays a key role in DNA repair, recombination, proliferation, and genomic stability. However, due to the poor electrochemical and optical activity of PARP-1 and its product PAR, only a few studies on its activity detection method have been reported. Herein, we report a simple and sensitive colorimetric strategy to monitor PARP-1 activity based on enzyme-initiated auto-PARylation-controlled aggregation of hemin-graphene nanocomposites (H-GNs)...
April 17, 2018: Analyst
https://www.readbyqxmd.com/read/29663528/purely-off-clamp-robotic-partial-nephrectomy-preliminary-3-year-oncological-and-functional-outcomes
#19
Giuseppe Simone, Leonardo Misuraca, Gabriele Tuderti, Francesco Minisola, Mariaconsiglia Ferriero, Giuseppe Romeo, Manuela Costantini, Samer F Al-Rawashdah, Salvatore Guaglianone, Michele Gallucci
OBJECTIVES: To describe our surgical technique and to report perioperative, 3-year oncological and functional outcomes of a single-center series of purely off-clamp robotic partial nephrectomy. METHODS: A prospective renal cancer institutional database was queried, and data of consecutive patients treated with purely off-clamp robotic partial nephrectomy between 2010 and 2015 in a high-volume center were collected. Perioperative complications, and 3-year oncological and functional outcomes were assessed...
April 16, 2018: International Journal of Urology: Official Journal of the Japanese Urological Association
https://www.readbyqxmd.com/read/29663029/yttrium-90-ibritumomab-tiuxetan-zevalin-followed-by-beam-z-beam-conditioning-regimen-and-autologous-stem-cell-transplantation-asct-in-relapsed-or-refractory-high-risk-b-cell-non-hodgkin-lymphoma-nhl-a-single-institution-italian-experience
#20
Chiara Ciochetto, Barbara Botto, Roberto Passera, Marilena Bellò, Giulia Benevolo, Carola Boccomini, Alessia Castellino, Annalisa Chiappella, Roberto Freilone, Maura Nicolosi, Lorella Orsucci, Clara Pecoraro, Patrizia Pregno, Gianni Bisi, Umberto Vitolo
Chemo-refractory NHL has a very poor outcome; the addiction of RIT to salvage regiment pre ASCT had recently demonstrated promising results.We performed a retrospective sequential study to determine the feasibility of standard Zevalin with BEAM in high-risk relapse/refractory NHL. A matched cohort analysis with a group treated with standard BEAM without Zevalin was performed as secondary endpoint. Between October 2006 and January 2013, 37 NHL patients at high risk for progression or early (< 1 year) or multiple relapses were treated with Z-BEAM and ASCT after R-DHAP or R-ICE as salvage therapy...
April 16, 2018: Annals of Hematology
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