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https://www.readbyqxmd.com/read/28528498/lipopeptides-produced-by-bacillus-subtilis-as-new-biocontrol-products-against-fusariosis-in-ornamental-plants
#1
Gabriela Mihalache, Tiberius Balaes, Irina Gostin, Marius Stefan, François Coutte, François Krier
In this study, we have investigated the effects of three lipopeptides (fengycin, surfactin and mycosubtilin) produced by different strains of Bacillus subtilis against the phytopathogenic fungi Fusarium oxysporum f. sp. iridacearum, which affects the ornamental bulb plant populations of Iris sp. The antifungal effects were tested using minimum inhibitory concentration assay, determination of mycelium growth and spore germination inhibition rates. Also, in vivo tests on infected rhizomes and scanning electron microscopy were employed...
May 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28528421/end-of-life-care-for-glioblastoma-patients-at-a-large-academic-cancer-center
#2
Kamini E Kuchinad, Roy Strowd, Anne Evans, W Anthony Riley, Thomas J Smith
Glioblastoma (GBM) is a universally fatal disease, complicated by significant cognitive and physical disabilities, inherent to the disease course. The purpose of this study was to retrospectively analyze end-of-life care for GBM patients at an academic center and compare utilization of these services to national quality of care guidelines, with the goal of identifying opportunities to improve end-of-life care. Single center retrospective cohort study of GBM patients at Johns Hopkins Hospital (JHH) between 2009 and 2014, using electronic medical records and hospice records...
May 20, 2017: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/28528396/penetration-of-vancomycin-into-the-cerebrospinal-fluid-a-systematic-review
#3
REVIEW
Jessica E Beach, Jerrold Perrott, Ricky D Turgeon, Mary H H Ensom
INTRODUCTION: Infectious disease and pharmacokinetic textbooks indicate that vancomycin has poor penetration into the central nervous system due to its hydrophilic nature and high molecular weight. Recent literature suggests that penetration of vancomycin into cerebrospinal fluid (CSF) is higher than previously reported; therefore, we conducted a systematic review to assess the penetration of vancomycin into CSF. METHODS: We searched the MEDLINE, EMBASE, and CENTRAL electronic databases for English-language human studies evaluating serum and CSF concentrations of intravenous vancomycin...
May 20, 2017: Clinical Pharmacokinetics
https://www.readbyqxmd.com/read/28528395/enhancing-hair-growth-in-male-androgenetic-alopecia-by-a-combination-of-fractional-co2-laser-therapy-and-hair-growth-factors
#4
Yue Huang, Fenglin Zhuo, Linfeng Li
Laser therapy and growth factors have been used as alternative treatments for male androgenetic alopecia (MAA). The aim of this study is to determine the efficacy and safety of hair growth factors alone or combined with ablative carbon dioxide (CO2) fractional laser therapy in MAA. Twenty-eight men were enrolled in this randomized half-split study based on a left-head to right-head pattern. Fractional CO2 laser treatment was unilaterally performed; hair growth factors were bilaterally applied. Six sessions with 2-week intervals were performed...
May 21, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28528370/systematic-radiographic-evaluation-of-three-potential-implantation-sites-for-a-semi-implantable-bone-conduction-device-in-52-patients-after-previous-mastoid-surgery
#5
Bernhard G Weiss, Mattis Bertlich, Rebecka Scheele, Martin Canis, Mark Jakob, Jan M Sohns, Friedrich Ihler
The aim of this study was the evaluation of three localizations for the implantation of a semi-implantable transcutaneous bone conduction device after previous mastoid surgery. This is a retrospective review of electronic datasets of cranial computed tomography studies. The study setting is one tertiary referral center and included 52 consecutive adult patients (60 temporal bones) with a history of mastoid surgery. The intervention was virtual placement of the device with a planning software within the remaining mastoid as well as dorsal of the sigmoid sinus and caudal of the transverse sinus (retrosigmoidal localization) and dorsocranial of the parietomastoid suture and cranial of the transverse sinus (parietal localization)...
May 20, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28528367/evaluating-the-role-of-autologous-mesenchymal-stem-cell-seeded-on-decellularized-pericardium-in-the-treatment-of-myocardial-infarction-an-animal-study
#6
Abdol-Mohammad Kajbafzadeh, Seyed Hossein Ahmadi Tafti, Reza Khorramirouz, Shabnam Sabetkish, Seyedeh Maryam Kameli, Saghar Orangian, Shahram Rabbani, Nasim Oveisi, Marziyeh Golmohammadi, Zeynab Kashani
Inappropriate left ventricular remodeling following myocardial infarction (MI) can result in subsequent severe dysfunction. In this study, we tested the hypothesis that decellularized pericardium (DP) or seeded pericardial patch with autologous adipose-derived mesenchymal stem cells (ADMSCs) could be safely used in a MI scar and could improve heart function. Twelve rabbits were randomly divided into three equal groups. Four weeks after MI induction by ligation of the left anterior descending artery in 12 rabbits, animals of G1 (n = 4) received DP patch with labeled ADMSCs...
May 20, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28528289/platelet-hyperactivation-apoptosis-and-hypercoagulability-in-patients-with-acute-pulmonary-embolism
#7
Samin Rezania, Michael A Puskarich, Daniela N Petrusca, Evandro M Neto-Neves, Matthew T Rondina, Jeffrey A Kline
Changes in systemic redox balance can alter platelet activation and aggregation. Acute pulmonary embolism (PE) is a systematic inflammatory disease associated with mechanical shear stress, increased thrombin, catecholamines, serotonin and hemolysis, which cumulatively can hyperactivate platelets and accelerate their turnover. We tested the hypothesis that platelets from patients with moderately severe PE will show hyperstimulation and a pre-apoptotic phenotype associated with microparticles (MPs) in plasma...
May 13, 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28528255/molecular-structure-second-and-third-order-nonlinear-optical-properties-and-dft-studies-of-a-novel-non-centrosymmetric-chalcone-derivative-2e-3-4-fluorophenyl-1-4-1e-4-fluorophenyl-methylene-amino-phenyl-prop-2-en-1-one
#8
Shivaraj R Maidur, Parutagouda Shankaragouda Patil, Anusha Ekbote, Tze Shyang Chia, Ching Kheng Quah
In the present work, the title chalcone, (2E)-3-(4-fluorophenyl)-1-(4-{[(1E)-(4-fluorophenyl) methylene]amino}phenyl)prop-2-en-1-one (abbreviated as FAMFC), was synthesized and structurally characterized by single-crystal X-ray diffraction. The compound is crystallized in the monoclinic system with non-centrosymmetric space group P21 and hence it satisfies the essential condition for materials to exhibit second-order nonlinear optical properties. The molecular structure was further confirmed by using FT-IR and (1)H NMR spectroscopic techniques...
May 9, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28528213/optimal-electrostatic-interactions-between-substrate-and-protein-are-essential-for-radical-chemistry-in-ornithine-4-5-aminomutase
#9
Caitlyn Makins, Douglas A Whitelaw, Michael McGregor, Alix Petit, Robert G Mothersole, Kathleen E Prosser, Kirsten R Wolthers
Ornithine 4,5-aminomutase (OAM) from Clostridium sticklandii is an adenosylcobalamin (AdoCbl) and pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes a 1,2-amino shift, interconverting D-ornithine and 2S, 4R-diaminopentanoate. The reaction occurs via a radical-based mechanism whereby a PLP-bound substrate radical undergoes intramolecular isomerization via an azacyclopropylcarbinyl radical intermediate. Herein, we investigated the catalytic role of active site residues that form non-covalent interactions with PLP and/or substrate, D-ornithine...
May 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28528210/do-patients-need-to-stay-in-bed-all-day-in-the-epilepsy-monitoring-unit-safety-data-from-a-non-restrictive-setting
#10
Laura Craciun, Jørgen Alving, Elena Gardella, Daniella Terney, Pirgit Meritam, Melita Cacic Hribljan, Sándor Beniczky
PURPOSE: To assess whether injuries occur more often in an Epilepsy Monitoring Unit (EMU) where portable EEG amplifiers are used, and where patients can freely move within a large area during the monitoring. METHODS: Patients were monitored at the Danish Epilepsy Center, in an EMU specifically designed for this purpose, and they were under continuous surveillance by personnel dedicated to the EMU. Adverse events (AEs) - including injuries, were prospectively noted, as part of the safety policy of the hospital...
May 13, 2017: Seizure: the Journal of the British Epilepsy Association
https://www.readbyqxmd.com/read/28528208/mentoring-interventions-and-the-impact-of-protective-assets-on-the-reproductive-health-of-adolescent-girls-and-young-women
#11
REVIEW
Kate F Plourde, Nicole B Ippoliti, Geeta Nanda, Donna R McCarraher
Adolescent girls and young women (AGYW) are disproportionately affected by HIV and AIDS and other negative reproductive health (RH) outcomes. Emerging evidence suggests that programs to build AGYW's assets can help reduce their vulnerability to poor RH. Mentoring interventions have demonstrated a positive impact on a variety of youth development outcomes, including the protective assets needed to circumvent poor RH outcomes. The purpose of this review was to understand the types of mentoring programs for AGYW that have demonstrated effectiveness in improving protective assets, and/or, RH knowledge, intentions, behaviors, or outcomes themselves...
May 17, 2017: Journal of Adolescent Health: Official Publication of the Society for Adolescent Medicine
https://www.readbyqxmd.com/read/28528203/changes-in-resting-state-functional-brain-connectivity-and-withdrawal-symptoms-are-associated-with-acute-electronic-cigarette-use
#12
Andréa L Hobkirk, Travis T Nichols, Jonathan Foulds, Jessica M Yingst, Susan Veldheer, Shari Hrabovsky, John Richie, Thomas Eissenberg, Stephen J Wilson
Resting state functional brain connectivity (rsFC) may be an important neuromarker of smoking behavior. Prior research has shown, among cigarette smokers, that nicotine administration alters rsFC within frontal and parietal cortices involved in executive control, as well as striatal regions that drive reward processing. These changes in rsFC have been associated with reductions in withdrawal symptom severity. We currently have a limited understanding of how rsFC is affected by the use of electronic cigarettes (ecigs), an increasingly popular class of products, the members of which deliver nicotine with varying effectiveness...
May 17, 2017: Brain Research Bulletin
https://www.readbyqxmd.com/read/28528175/heterodimeric-v-nikolskii-phospholipases-a2-induce-aggregation-of-the-lipid-bilayer
#13
A S Alekseeva, D S Tretiakova, V P Chernikov, Yu N Utkin, Yu G Molotkovskii, E L Vodovozova, I A Boldyrev
We report that the action of the heterodimeric phospholipases A2 (PLA2s) from Vipera nikolskii, which comprises enzymatically active basic subunit and inactive acidic PLA2 homologue, on the lipid bilayer results in the aggregation and stacking of bilayers. These processes are demonstrated using two independent methods (fluorescence spectroscopy and electron microscopy). Aggregation of bilayers is possible because both subunits of the V. nikolskii heterodimer contain a membrane-binding site (also known as IBS)...
May 17, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28528145/uv-induced-electron-transfer-between-triethylamine-and-5-bromo-2-deoxyuridine-a-puzzle-concerning-the-photochemical-debromination-of-labeled-dna
#14
Paweł Wityk, Magdalena Zdrowowicz, Justyna Wiczk, Janusz Rak
5-Bromo-2'-deoxyuridine (BrdU) photosensitizes DNA to strand break formation. However, this type of photodamage is completely quenched by the presence of triethylamine (TEA) which originates from RP-HPLC purification commonly employed by oligonucleotide providers. While the presence of TEA in oligonucleotide samples does not interfere with PCR or other molecular biology applications, the mechanism of photochemical reaction proceeding in the labeled DNA is dramatically changed due to the photoinduced electron transfer (PET) between the photoexcited BrdU and the ground state TEA...
May 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28528135/presymptomatically-applied-ampa-receptor-antagonist-prevents-calcium-increase-in-vulnerable-type-of-motor-axon-terminals-of-mice-modeling-amyotrophic-lateral-sclerosis
#15
Roland Patai, Melinda Paizs, Massimo Tortarolo, Caterina Bendotti, Izabella Obál, József I Engelhardt, László Siklós
Increased intracellular calcium (Ca), which might be the consequence of an excess influx through Ca-permeable α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, plays a crucial role in degeneration of motor neurons. Previously we demonstrated that the presymptomatic application of AMPA receptor antagonist, talampanel, could reduce Ca elevation in spinal motor neurons of mice carrying the G93A mutation of superoxide dismutase 1 (SOD1), modeling amyotrophic lateral sclerosis (ALS). It remained to be examined whether the remote, functionally semi-autonomous motor axon terminals could be rescued from the Ca overload, or if the terminals, where the degeneration possibly starts, already experience intractable changes at early time points...
May 17, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28528134/enhancing-tumor-cell-response-to-multidrug-resistance-with-ph-sensitive-quercetin-and-doxorubicin-conjugated-multifunctional-nanoparticles
#16
Cenk Daglioglu
Classical chemotherapy uses chemotherapeutic agents as a mainstay of anticancer treatment. However, the development of multidrug resistance to chemotherapy limits the effectiveness of current cancer treatment. Nanosized bioconjugates combining a chemotherapeutic agent with a pharmacological approach may improve the curative effect of chemotherapeutic agents. Herein I addressed this issue by describing the synthesis, and testing of, pH-responsive Fe3O4@SiO2(FITC)-BTN/QUR/DOX multifunctional nanoparticles. The particles were designed to modulate resistance-mediating factors and to potentiate the efficacy of DOX against chemoresistance...
May 10, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28528133/chemical-synthesis-of-flower-like-hybrid-cu-oh-2-cuo-electrode-application-of-polyvinyl-alcohol-and-triton-x-100-to-enhance-supercapacitor-performance
#17
S K Shinde, V J Fulari, D-Y Kim, N C Maile, R R Koli, H D Dhaygude, G S Ghodake
In this research article, we report hybrid nanomaterials of copper hydroxide/copper oxide (Cu(OH)2/CuO). A thin films were prepared by using a facile and cost-effective successive ionic layer adsorption and reaction (SILAR) method. As-synthesized and hybrid Cu(OH)2/CuO with two different surfactants polyvinyl alcohol (PVA) and triton-X 100 (TRX-100) was prepared having distinct morphological, structural, and supercapacitor properties. The surface of the thin film samples were examined by scanning electron microscopy (SEM)...
May 9, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28528131/no-increase-in-the-incidence-of-acute-kidney-injury-in-a-population-based-annual-temporal-trends-epidemiology-study
#18
Kianoush Kashani, Min Shao, Guangxi Li, Amy W Williams, Andrew D Rule, Walter K Kremers, Michael Malinchoc, Ognjen Gajic, John C Lieske
Recent literature suggests an increase in the incidence of acute kidney injury (AKI). We evaluated population-based trends of AKI over the course of nine years, using a validated electronic health record tool to detect AKI. All adult residents (18 years of age and older) of Olmsted County, Minnesota (MN), admitted to the Mayo Clinic Hospital between 2006 and 2014 were included. The incidence rate of AKI was calculated and temporal trends in the annual AKI incident rates assessed. During the nine-year study period, 10,283, and 41,847 patients were admitted to the intensive care unit or general ward, with 1,740 and 2,811 developing AKI, respectively...
May 18, 2017: Kidney International
https://www.readbyqxmd.com/read/28528002/iron-microencapsulation-in-gum-tragacanth-using-solvent-evaporation-method
#19
Elham Asghari-Varzaneh, Mohammad Shahedi, Hajar Shekarchizadeh
In this study iron salt (FeSO4·7H2O) was microencapsulated in gum tragacanth hydrogel using solvent evaporation method. Three significant parameters (ferrous sulfate content, content of gum tragacanth, and alcohol to mixture ratio) were optimized by response surface methodology to obtain maximum encapsulation efficiency. Ferrous sulfate content, 5%, content of gum tragacanth, 22%, and alcohol to mixture ratio, 11:1 was determined to be the optimum condition to reach maximum encapsulation efficiency. Microstructure of iron microcapsules was thoroughly monitored using scanning electron microscopy (SEM)...
May 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28527981/an-identification-of-the-soft-polyelectrolyte-gel-like-layer-on-silica-colloids-using-atomic-force-and-electron-microscopy
#20
Jiří Škvarla, Juraj Škvarla
A procedure is introduced for measuring the radius of spherical colloid particles from the curvature of upper parts of their central cross-sectional profiles obtained by atomic force microscopy (AFM). To minimize the possible compression and displacement of the spheres, AFM is operated in a mode rendering a constant ultralow pN force on the tip. The procedure allows us to evaluate the mean radius of nearly monodisperse submicrometer spheres of silica in their natively hydrated state in aqueous electrolyte solutions, irrespective of whether they are coagulated or not...
May 16, 2017: Ultramicroscopy
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