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https://www.readbyqxmd.com/read/28738660/novel-biomimetic-enzyme-for-sensitive-detection-of-superoxide-anions
#1
Fang Peng, Tingting Xu, Fan Wu, Chunxue Ma, Yuhong Liu, Jianying Li, Bo Zhao, Chun Mao
Superoxide anion (O2(•-)), one of the most active reactive oxygen species (ROS) in micro-environment of the human body, is involved in some diseases if there is excess O2(•-) associated with oxidative stress. Accurate detection of its concentration has important medical diagnostic significance. In this work, a new electrochemical sensor was designed and fabricated for sensitive detection based on Mn-superoxide dismutase (MnSOD) that decorated onto the surface of magnetic polymeric nanotubes by surface self-assembly processes...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738658/an-ultrasensitive-chemiluminescence-aptasensor-for-thrombin-detection-based-on-iron-porphyrin-catalyzing-luminescence-desorbed-from-chitosan-modified-magnetic-oxide-graphene-composite
#2
Yuanling Sun, Yanhui Wang, Jianbo Li, Chaofan Ding, Yanna Lin, Weiyan Sun, Chuannan Luo
In this work, an ultrasensitive chemiluminescence (CL) aptasensor was prepared for thrombin detection based on iron porphyrin catalyzing luminol - hydrogen peroxide luminescence under alkaline conditions, and iron porphyrin was desorbed from chitosan modified magnetic oxide graphene composite (CS@Fe3O4@GO). Firstly, CS@Fe3O4@GO was prepared. CS@Fe3O4@GO has advantages of the good biocompatibility and positively charged on its surface of CS, the large specific surface area of GO and the easy separation characteristics of Fe3O4...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738646/synthesis-characterization-and-application-of-a-novel-multifunctional-stationary-phase-for-hydrophilic-interaction-reversed-phase-mixed-mode-chromatography
#3
Hayriye Aral, K Serdar Çelik, Ramazan Altındağ, Tarık Aral
A novel multifunctional stationary phase based on silica gel was synthesised starting from L- isoleucine and 4-phenylbutylamine and evaluated as a hydrophilic interaction/reversed-phase mixed-mode stationary phase for high-performance liquid chromatography (HPLC). The prepared stationary phase was characterized by elemental analysis, infrared spectroscopy (IR), scanning electron microscopy (SEM), Brunauer, Emmett and Teller (BET) and solid-state (13)C nuclear magnetic resonance (NMR). The mechanisms involved in the chromatographic separation are multi-interaction, including hydrophobic, π-π, hydrogen-bonding, dipole-dipole and ion-dipole interactions...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738635/microwave-induced-combustion-thermal-and-morphological-aspects-for-understanding-the-mechanism-of-ignition-process-for-analytical-applications
#4
Matheus F Pedrotti, Leticia S F Pereira, Cezar A Bizzi, Jose N G Paniz, Juliano S Barin, Erico M M Flores
In the present work, for the first time a systematic study was performed using an infrared camera and scanning electron microscopy (SEM) coupled to energy dispersive X-ray spectrometry (EDS) to evaluate the mechanisms involved in microwave-induced combustion method, which has been extensively used for sample preparation. Cellulose and glass fiber discs, wetted with the igniter solution (6molL(-1) NH4NO3), were evaluated under microwave field in a monomode system. The temperature of the discs surface was recorded during microwave irradiation and the effect of NH4NO3 concentration and irradiation time on cellulose oxidation was evaluated...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738630/simultaneous-determination-of-paracetamol-and-ciprofloxacin-in-biological-fluid-samples-using-a-glassy-carbon-electrode-modified-with-graphene-oxide-and-nickel-oxide-nanoparticles
#5
Anderson Martin Santos, Ademar Wong, Adriana Araújo Almeida, Orlando Fatibello-Filho
A simple and highly selective electrochemical method using a glassy carbon electrode (GCE) modified with graphene oxide (GO) and nickel oxide nanoparticles (NiONPs) was developed for the simultaneous determination of paracetamol (PAR) and ciprofloxacin (CIP). The electrochemical characterisation of the modified GCE was performed by cyclic voltammetry and electrochemical impedance spectroscopy. The morphological characterisation of the GO and NiONPs was performed by scanning electron microscopy and transmission electron microscopy...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738619/simultaneous-and-sensitive-electrochemical-detection-of-dihydroxybenzene-isomers-with-uio-66-metal-organic-framework-mesoporous-carbon
#6
Min Deng, Shourui Lin, Xiangjie Bo, Liping Guo
The zirconium-based MOF (UiO-66)/mesoporous carbon (MC) composite was synthesized using conventional hydrothermal method for the first time. The surface morphology and structure of UiO-66/MC composite were characterized by scanning electron microscopy (SEM), transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). A novel electrochemical sensor based on UiO-66/MC was constructed for simultaneous and sensitive determination of dihydroxybenzene isomers (DBIs) of hydroquinone (HQ), catechol (CT) and resorcinol (RS)...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738614/in-situ-synthesis-of-reduced-graphene-oxide-gold-nanoparticles-modified-electrode-for-speciation-analysis-of-copper-in-seawater
#7
Ming Liu, Dawei Pan, Wen Pan, Yun Zhu, Xueping Hu, Haitao Han, Chenchen Wang, Dazhong Shen
In this paper, reduced graphene oxide/gold nanoparticle/Nafion (rGO/AuNP/Nafion) modified electrode was fabricated through in-situ synthesis and used for determination of copper ions in strong salt electrolyte (0.5M NaCl) by differential pulse anodic stripping voltammetry (DPASV). This in-situ synthesis procedure was based on the ion-exchange and electrochemical reduction. The use of rGO provided a larger electrode surface and the presence of more active sites, while electron transfer was accelerated by incorporating AuNPs...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738593/boron-nitride-nanosheets-as-a-platform-for-fluorescence-sensing
#8
Yuanjin Zhan, Jingjing Yan, Mei Wu, Longhua Guo, Zhenyu Lin, Bin Qiu, Guonan Chen, Kwok-Yin Wong
Hexagonal boron nitride nanosheets (BNNS), one kind of inorganic graphene analogue, exhibit high fluorescence quenching ability via the photo-induced electron transfer (PET) and different affinity toward single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). As a proof of concept, the BNNS was first used as a sensing platform for the rapid detection of DNA and small molecules with high sensitivity and selectivity in this study. This strategy is versatile and quick fluorescence sensing of DNA and extensive DNA related analytes such as metal cations and small molecules...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738585/ordered-mesoporous-silica-film-as-a-novel-fiber-coating-for-solid-phase-microextraction
#9
Hui Jiang, Jiansheng Li, Xingru Hu, Jinyou Shen, Xiuyun Sun, Weiqing Han, Lianjun Wang
Ordered mesoporous silica (OMS) film supported on anodized titanium wire was fabricated as a new solid phase microextraction (SPME) fiber for the extraction of chlorophenols (CPs) from aqueous media followed by the determination with gas chromatography flame ionization detection (GC-FID). The supported OMS film was prepared via a solvent evaporation-induced self-assembly approach (EISA) combined with dip-coating technology. Commonly used fragile fused silica fiber was replaced by anodized titanium wire. The coating materials were characterized by N2 adsorption-desorption, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric (TG) analysis...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738579/compositional-engineering-of-vopcpho-tio2-nano-composite-to-reduce-the-absolute-threshold-value-of-humidity-sensors
#10
Mohamad Izzat Azmer, Fakhra Aziz, Zubair Ahmad, Ehsan Raza, Mansoor Ani Najeeb, Noshin Fatima, Tahani M Bawazeer, Mohammad S Alsoufi, R A Shakoor, Khaulah Sulaiman
This research work demonstrates compositional engineering of an organic-inorganic hybrid nano-composites for modifying absolute threshold of humidity sensors. Vanadyl-2,9,16,23-tetraphenoxy-29H,31H-phthalocyanine (VOPcPhO), an organic semiconductor, doped with Titanium-dioxide nanoparticles (TiO2 NPs) has been employed to fabricate humidity sensors. The morphology of the VOPcPhO:TiO2 nano-composite films has been analyzed by atomic force microscopy (AFM) and field emission scanning electron microscopy (FESEM)...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738574/a-highly-selective-and-sensitive-fluorescent-probe-for-hypochlorous-acid-and-its-lysosome-targetable-biological-applications
#11
Chang Liu, Xiaojie Jiao, Song He, Liancheng Zhao, Xianshun Zeng
A novel lysosome-targetable fluorescence probe PT-1 based on a photoinduced electron transfer (PET) mechanism has been designed and synthesized. For comparison, the probe PT-2 without morpholine moiety as a lysosome-directing group was also synthesized and investigated. Probes PT-1 and PT-2 exhibited high selectivity, high sensitivity (with the detection limits down to the 10(-10)M range) and response in real time (within 10s) toward HOCl over other reactive oxygen species (ROS). Both PT-1 and PT-2 were cell permeable and enabled them to be used for monitoring of HOCl in living cells...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738568/novel-imidazole-fluorescent-poly-ionic-liquid-nanoparticles-for-selective-and-sensitive-determination-of-pyrogallol
#12
Zhouyang Li, Yiwen Yang, Yanbo Zeng, Jianbo Wang, Haiqing Liu, Longhua Guo, Lei Li
This paper reports novel imidazole fluorescent poly(ionic liquid) nanoparticles (FPILNs) of poly(1-[(4-methyphenyl)methyl]-3-vinyl-imidazolium bromide (poly([MVI]Br) for selective and sensitive determination of pyrogallol. An imidazole ionic liquid of 1-[(4-methyphenyl)methyl]-3-vinyl-imidazolium bromide ([MVI]Br) was synthesized and used as the only monomer to obtain poly([MVI]Br) possessing phenyl fluorophores using a radical polymerization technique. The obtained poly([MVI]Br) can form nanoparticles in water...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738566/preparation-and-evaluation-of-a-novel-n-benzyl-phenethylamino-%C3%AE-cyclodextrin-bonded-chiral-stationary-phase-for-hplc
#13
Liang Li, Biaoping Cheng, Rendan Zhou, Zhigang Cao, Chun Zeng, Laisheng Li
A novel N-benzyl-phenethylamino-β-cyclodextrin-bonded ordered mesoporous SBA-15 chiral stationary phase (BZCDP) for high-performance liquid chromatography (HPLC) was prepared. The structure and morphology of the ligand and the stationary phase were characterized by mass spectrometry, elemental analysis, infrared spectroscopy, scanning electron microscopy, transmission electron microscopy and thermogravimetric analysis. The enantiomers of chiral compounds including nine common β-adrenergic blocker drugs (β-blockers), eight dansyl amino acids (DNS-AA) and six flavanones were successfully separated by polar organic solvent and reversed-phase chromatography, respectively...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738559/a-novel-dianionic-amino-acid-ionic-liquid-coated-peg-4000-modified-fe3o4-nanocomposite-for-the-magnetic-solid-phase-extraction-of-trypsin
#14
Qin Yang, Yuzhi Wang, Hongmei Zhang, Kaijia Xu, Xiaoxiao Wei, Panli Xu, Yigang Zhou
A novel magnetic extractant, PEG 4000 modified Fe3O4nanomaterial that coated with dianionic amino acid ionic liquid (Fe3O4@PEG@DAAAIL), was successfully synthesized and characterized. X-ray diffraction (XRD), transmission electron microscope (TEM), vibrating sample magnetometer (VSM), fourier transform infrared spectrometry (FT-IR), thermal gravimetric analysis (TGA) and zeta potentials were used to confirm that the novel nanocomposite was successfully synthesized. Subsequently, the prepared Fe3O4@PEG@DAAAIL nanocomposite was used as the extractant for trypsin coupled with magnetic solid-phase extraction (MSPE)...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738544/a-xylanase-aided-enzymatic-pretreatment-facilitates-cellulose-nanofibrillation
#15
Lingfeng Long, Dong Tian, Jinguang Hu, Fei Wang, Jack Saddler
Although biological pretreatment of cellulosic fiber based on endoglucanases has shown some promise to facilitate cellulose nanofibrillation, its efficacy is still limited. In this study, a xylanase-aided endoglucanase pretreatment was assessed on the bleached hardwood and softwood Kraft pulps to facilitate the downstream cellulose nanofibrillation. Four commercial xylanase preparations were compared and the changes of major fiber physicochemical characteristics such as cellulose/hemicellulose content, gross fiber properties, fiber morphologies, cellulose accessibility/degree of polymerization (DP)/crystallinity were systematically evaluated before and after enzymatic pretreatment...
July 9, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28738525/mesenchymal-stem-cells-with-igf-1-and-tgf-%C3%AE-1-in-laminin-gel-for-osteochondral-defects-in-rabbits
#16
Mudasir Bashir Gugjoo, Amarpal, Ahmed Abdelbaset-Ismail, Hari Prasad Aithal, Prakash Kinjavdekar, Abhijit Motiram Pawde, Gutulla Sai Kumar, Gutulla Taru Sharma
OBJECTIVE: Healing of articular cartilage is still a challenge due to its limited potential to regenerate. In the present study, we evaluated allogenic bone marrow mesenchymal stem cells (BM-MSCs) alone or in combination with growth factors, insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1) in laminin scaffolds for healing of osteochondral defects. DESIGN: Osteochondral defects of 4mm (diameter) x 5mm (depth) were induced in the rabbit knee joints and treated with phosphate-buffered saline (PBS; control), BM-MSCs, BM-MSCs in laminin, BM-MSCs in laminin with IGF-1, or BM-MSCs in laminin with IGF-1 and TGF-β1 in 10 animals each...
July 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738521/ginkgo-biloba-exocarp-extracts-induces-autophagy-in-lewis-lung-cancer-cells-involving-ampk-mtor-p70s6k-signaling-pathway
#17
Chenjie Cao, Dongdong Han, Ya Su, Yu Ge, Huasheng Chen, Aihua Xu
OBJECTIVES: Ginkgo biloba L. is called a living fossil plant, and could be used for the treatment of cancer thousands of years ago in China. The extracts prepared from the Ginkgo biloba exocarp (Ginkgo biloba exocarp extracts, GBEE) has a significant anti-cancer effect. Autophagy plays an important role in the occurrence and development of cancer as programmed cell death (PCD) type II. Thus it would be interesting to study the effects and mechanisms of GBEE inducing autophagy in Lewis lung cancer (LLC) cells...
July 19, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738425/preparation-and-characterization-of-pegylated-iron-oxide-gold-nanoparticles-for-delivery-of-sulforaphane-and-curcumin
#18
Hossein Danafar, Ali Sharafi, Sonia Askarlou, Hamidreza Kheiri Manjili
Natural products have been used for the treatment of various diseases such as cancer. Curcumin (CUR) and sulforaphane (SF) have anti-cancer effects, but their application is restricted because of their low water solubility and poor oral bioavailability. To improve the bioavailability and solubility of SF and CUR, we performed an advanced delivery of SF and CUR with PEGylated gold coated Fe3O4 magnetic nanoparticles (PEGylated Fe3O4@Au NPs) to endorse SF and CUR maintenance as an effective and promising antitumor drugs...
July 24, 2017: Drug Research
https://www.readbyqxmd.com/read/28738423/continued-statin-prescriptions-after-adverse-reactions-and-patient-outcomes-a-cohort-study
#19
Huabing Zhang, Jorge Plutzky, Maria Shubina, Alexander Turchin
Background: Many patients discontinue statin treatment, often after having a possible adverse reaction. The risks and benefits of continued statin therapy after an adverse reaction are not known. Objective: To examine the relationship between continuation of statin therapy (any prescription within 12 months after an adverse reaction) and clinical outcomes. Design: Retrospective cohort study. Setting: Primary care practices affiliated with 2 academic medical centers...
July 25, 2017: Annals of Internal Medicine
https://www.readbyqxmd.com/read/28738399/use-of-smartphones-for-clinical-and-medical-education
#20
Jazmine Valle, Tyler Godby, David P Paul, Harlan Smith, Alberto Coustasse
Smartphone use in clinical settings and in medical education has been on the rise, benefiting both health care and health care providers. Studies have shown, however, that some health care facilities and providers are reluctant to switch to smartphones due to the threat of mixing personal apps with clinical care applications and the possibility that distraction created by smartphone use could lead to medication errors and errors linked to procedures, treatments, or tests. The purpose of this research was to examine the effects of smartphones in a clinical setting and for medical education, to determine their overall impact...
July 2017: Health Care Manager
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