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https://www.readbyqxmd.com/read/28812308/transcriptional-regulation-of-the-protein-kinase-a-subunits-in-saccharomyces-cerevisiae-during-fermentative-growth
#1
Fiorella Galello, Constanza Pautasso, Sol Reca, Luciana Cañonero, Paula Portela, Silvia Moreno, Silvia Rossi
Yeast cells can adapt their growth in response to the nutritional environment. Glucose is the favorite carbon source of Saccharomyces cerevisiae that prefers a fermentative metabolism despite the presence of oxygen. When glucose is consumed, the cell switches to the aerobic metabolism of ethanol, during the so-called diauxic shift. The difference between fermentative and aerobic growth is in part mediated by a regulatory mechanism called glucose repression. During glucose derepression a profound gene transcriptional reprogramming occurs and genes involved in the utilization of alternative carbon sources are expressed...
August 15, 2017: Yeast
https://www.readbyqxmd.com/read/28812184/ozone-generated-by-air-purifier-in-low-concentrations-friend-or-foe
#2
Larissa Vivan Cestonaro, Ana Maria Marcolan, Luciana Grazziotin Rossato-Grando, Ana Paula Anzolin, Gabriela Goethel, Angélica Vilani, Solange Cristina Garcia, Charise Dallazem Bertol
Ozone helps decontamination environments due to its oxidative power, however present toxicity when it is in high concentrations, by long periods of exposition. This study aimed to assess the safety of ozone generator air purifier at concentrations of 0.05 ppm in rats exposed to 3 and 24 h/day for 14 and 28 days. No significant differences are observed between groups in clinical signs, feed and water intake, relative body weight gain and relative weight of organs, macroscopy and microscopy of lungs, and oxidative plasma assay...
August 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28812109/vibratory-perineal-stimulation-for-the-treatment-of-female-stress-urinary-incontinence-a-systematic-review
#3
Marina Petter Rodrigues, Luciana Laureano Paiva, José Geraldo Lopes Ramos, Lia Ferla
INTRODUCTION AND HYPOTHESIS: The pelvic floor muscles (PFM) play an important part in the urinary continence mechanism. Changes in their structure and functionality may lead to a predisposition to pelvic floor dysfunction such as urinary incontinence (UI), which is the involuntary loss of urine. Some techniques for conservative treatment of UI are already well documented. However, new approaches have been found that require scientific proof of their effectiveness, such as vibratory stimulation (VS)...
August 15, 2017: International Urogynecology Journal
https://www.readbyqxmd.com/read/28810132/short-communication-alteration-in-blood-parameters-by-enrofloxacin-in-juvenile-lesser-spotted-dogfish-scyliorhinus-canicula-linnaeus-1758-after-intramuscular-injection
#4
Annalisa Zaccaroni, Marcos Perez-Lopez, Irene de la Casa Resino, Gianfranco Medri, Fabrizio Ceneri, Simone Bertini, Oliviero Mordenti, Luana Cortinovis, Rubina Sirri, Luciana Mandrioli
Enrofloxacin (EF) is a veterinary drug for respiratory, gastrointestinal and urinary tract infections. Parenteral administration at doses higher than the manufacturer recommended dosage has induced cartilage lesions in juvenile mammals and poultry. In elasmobranchs EF is commonly used for the treatment of infectious diseases, however only one study has been performed previously to evaluate pharmacokinetics and the potential chondrotoxicity of a fluoroquinolone in adult skates. In present study juvenile lesser spotted dogfish (Scyliorhinus canicula, Linnaeus, 1758) were treated with low (LD, 5mg/kg) and high dose (HD, 10mg/kg) of EF daily via intramuscular injection for 15 consecutive days, as indicated in a recognized treatment...
August 10, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28809462/multiple-electronic-and-structural-factors-control-cpd-and-6-4-thymine-thymine-photodimerization-in-dna-duplex
#5
Irene Conti, Lara Martinez Fernandez, Luciana Esposito, Siegfried Hofinger, Artur Nenov, Marco Garavelli, Roberto Improta
The T-T photodimerization paths leading to the formation of cyclobutane pyrimidine dimer (CPD) and 6-4 pyrimidine pyrimidone (64-PP), the two main DNA photolesions, have been resolved for a T-T step in a DNA duplex by two complementary state-of-the art quantum mechanical approaches: QM(CASPT2//CASSCF)/MM and TD-DFT/PCM. Based on the analysis of several different representative structures, we define a new-ensemble of cooperating geometrical and electronic factors (besides the distance between the reacting bonds) ruling T-T photodimerization in DNA...
August 15, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28808408/anti-lipid-potential-of-drimys-brasiliensis
#6
Josueli Merotto, Larissa Vivan Cestonaro, Luciana Grazziotin Rossato-Grando, Luciano de Oliveira Siqueira, Charise Dallazem Bertol
BACKGROUND: The traditional use of Drimys brasiliensis Miers (Winteraceae) in the south of Brazil to reduce cholesterol has not been described in scientific literature. OBJECTIVE: To verify the hypocholesterolemic effects of D. brasiliensis using rats as animal model. MATERIALS AND METHODS: The bark of D. brasiliensis was extracted with water with further lyophilization and was subjected to phytochemical analysis by high-performance liquid chromatography (HPLC), and free radical scavenging activities by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay to determine antioxidant potential...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28808407/in-vitro-cell-viability-by-cellprofiler-%C3%A2-software-as-equivalent-to-mtt-assay
#7
Luciana S Gasparini, Nayana D Macedo, Elisângela F Pimentel, Marcio Fronza, Valdemar L Junior, Warley S Borges, Eduardo R Cole, Tadeu U Andrade, Denise C Endringer, Dominik Lenz
OBJECTIVE: This study evaluated in vitro cell viability by the colorimetric MTT stands for 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay compared to image analysis by CellProfiler(®) software. MATERIALS AND METHODS: Hepatoma (Hepa-1c1c7) and fibroblast (L929) cells were exposed to isolated substances, camptothecin, lycorine, tazettine, albomaculine, 3-epimacronine, trispheridine, galanthine and Padina gymnospora, Sargassum sp...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28807290/exposure-to-vector-borne-pathogens-in-privately-owned-dogs-living-in-different-socioeconomic-settings-in-brazil
#8
Luciana Aguiar Figueredo, Kamila Gaudêncio da Silva Sales, Katrin Deuster, Matthias Pollmeier, Domenico Otranto, Filipe Dantas-Torres
This survey was conducted in four Brazilian cities, from three federative units (Pernambuco, Minas Gerais, and Federal District), representing different socioeconomic settings, to determine the presence of antibodies to or antigens and DNA of selected pathogens in privately owned dogs. From January to April 2015, blood and serum samples were collected and assayed using different tests. In particular, antibodies to Anaplasma spp., Borrelia burgdorferi sensu lato, and Ehrlichia spp., and antigens of Dirofilaria immitis were detected using a rapid enzyme-linked immunosorbent assay, whereas antibodies to Babesia spp...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28802387/sleep-quality-and-communication-aspects-in-children
#9
Camila de Castro Corrêa, Maria Renata José, Eduardo Carvalho Andrade, Mariza Ribeiro Feniman, Ana Paula Fukushiro, Giédre Berretin-Felix, Luciana Paula Maximino
OBJECTIVES: To correlate quality of life of children in terms of sleep, with their oral language skills, auditory processing and orofacial myofunctional aspects. METHODS: Nineteen children (12 males and seven females, in the mean age 9.26) undergoing otorhinolaryngological and speech evaluations participated in this study. The OSA-18 questionnaire was applied, followed by verbal and nonverbal sequential memory tests, dichotic digit test, nonverbal dichotic test and Sustained Auditory Attention Ability Test, related to auditory processing...
September 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28801810/effects-of-pbm-in-different-energy-densities-and-irradiance-on-maintaining-cell-viability-and-proliferation-of-pulp-fibroblasts-from-human-primary-teeth
#10
Nádia Carolina Teixeira Marques, Natalino Lourenço Neto, Mariel Tavares Oliveira Prado, Luciana Lourenço Ribeiro Vitor, Rodrigo Cardoso Oliveira, Vivien Thiemy Sakai, Carlos Ferreira Santos, Maria Aparecida Andrade Moreira Machado, Thais Marchini Oliveira
This study aimed to compare the effects of photobiomodulation (PBM) in different energy densities and irradiances on maintaining cell viability, and proliferation of pulp fibroblasts from human primary teeth (HPF) were cultured in DMEM and used between the fourth and eighth passages. Then, HPF were irradiated with the following different energy densities: 1.25 J/cm(2) (a), 2.50 J/cm(2) (b), 3.75 J/cm(2) (c), 5.00 J/cm(2) (d), and 6.25 J/cm(2) (e); but varying either the time of irradiation (groups 1a-1e) or the output power (groups 2a-2e)...
August 11, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28801728/arctium-minus-crude-extract-presents-antinociceptive-effect-in-a-mice-acute-gout-attack-model
#11
Susana Paula Moreira Fischer, Indiara Brusco, Camila Camponogara, Mariana Piana, Henrique Faccin, Luciana Assis Gobo, Leandro Machado de Carvalho, Sara Marchesan Oliveira
Gout is a disorder that triggers a severe inflammatory reaction which generates episodes of intense pain and discomfort to the patient. Arctium minus (Hill) Bernh. (Asteraceae) is known as "burdock" and displays anti-inflammatory, antinociceptive, against rheumatic pain and radical-scavenging activities. Species of the genus Arctium have been used in assistant therapy of gout and other inflammatory processes. We investigated the antinociceptive and anti-edematogenic effects of the crude extract of A. minus seeds in an acute gout attack model induced by intra-articular injection of monosodium urate (MSU) crystals in adult male Swiss mice (25-30 g)...
August 11, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/28800012/quality-assessment-of-prognostic-studies-using-cancer-stem-cell-markers-in-oral-squamous-cell-carcinoma
#12
Marina G Teixeira, Luciana Corrêa
Cancer stem cells (CSC) have been investigated as prognostic markers in oral squamous cell carcinoma (OSCC). However, an assessment of the reporting quality of these studies has not been performed yet. The aim of this study was to describe the reporting quality of prognostic studies involving CSCs and OSCC, focusing mainly on the immunohistochemical reproducibility. By means a systematic review, 34 articles were selected. Analyses of both general reporting quality and immunohistochemistry technique were performed by using checklists for multiple aspects related to study reproducibility...
August 9, 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28798917/corrigendum-biomarkers-and-algorithms-for-the-diagnosis-of-vitamin-b12-deficiency
#13
Luciana Hannibal, Vegard Lysne, Anne-Lise Bjørke-Monsen, Sidney Behringer, Sarah C Grünert, Ute Spiekerkoetter, Donald W Jacobsen, Henk J Blom
[This corrects the article on p. 27 in vol. 3, PMID: 27446930.].
2017: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/28797785/granulocyte-transfusions-a-critical-reappraisal
#14
REVIEW
Caterina Giovanna Valentini, Francesca Farina, Livio Pagano, Luciana Teofili
Granulocyte transfusions (GTs) are seldom used as a life-saving therapy for neutropenic patients with severe infections. Despite several compelling evidences of GT efficacy in retrospective and prospective case series, no study has been successful in demonstrating a definite advantage for recipients in controlled clinical trials. This review will critically revise some aspects emerging from the past experience which might have weakened the evidence of GT benefits. Some specific issues relevant to the efficacy of this therapeutic approach, such as the primary infection, the delivered doses and schedules, and the immunological effects of GTs, will be discussed...
August 7, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/28797083/the-transcriptional-response-to-the-olive-fruit-fly-bactrocera-oleae-reveals-extended-differences-between-tolerant-and-susceptible-olive-olea-europaea-l-varieties
#15
Filomena Grasso, Mariangela Coppola, Fabrizio Carbone, Luciana Baldoni, Fiammetta Alagna, Gaetano Perrotta, Antonio J Pérez-Pulido, Antonio Garonna, Paolo Facella, Loretta Daddiego, Loredana Lopez, Alessia Vitiello, Rosa Rao, Giandomenico Corrado
The olive fruit fly Bactrocera oleae (Diptera: Tephritidae) is the most devastating pest of cultivated olive (Olea europaea L.). Intraspecific variation in plant resistance to B. oleae has been described only at phenotypic level. In this work, we used a transcriptomic approach to study the molecular response to the olive fruit fly in two olive cultivars with contrasting level of susceptibility. Using next-generation pyrosequencing, we first generated a catalogue of more than 80,000 sequences expressed in drupes from approximately 700k reads...
2017: PloS One
https://www.readbyqxmd.com/read/28794847/neuro-otological-and-peripheral-nerve-involvement-in-fabry-disease
#16
Sergio Carmona, Romina Weinschelbaum, Ana Pardal, Cintia Marchesoni, Paz Zuberbuhler, Patricia Acosta, Guillermo Cáceres, Isaac Kisinovsky, Luciana Bayón, Ricardo Reisin
Fabry disease (FD) is an X-linked lysosomal storage disease, with multisystemic glycosphingolipids deposits. Neuro-otological involvement leading to hearing loss and vestibular dysfunctions has been described, but there is limited information about the frequency, site of lesion, or the relationship with peripheral neuropathy. The aim was to evaluate the presence of auditory and vestibular symptoms, and assess neurophysiological involvement of the VIII cranial nerve, correlating these findings with clinical and neurophysiological features of peripheral neuropathy...
July 18, 2017: Audiology Research
https://www.readbyqxmd.com/read/28794497/the-trophic-role-of-a-large-marine-predator-the-tiger-shark-galeocerdo-cuvier
#17
Luciana C Ferreira, Michele Thums, Michael R Heithaus, Adam Barnett, Kátya G Abrantes, Bonnie J Holmes, Lara M Zamora, Ashley J Frisch, Julian G Pepperell, Derek Burkholder, Jeremy Vaudo, Robert Nowicki, Jessica Meeuwig, Mark G Meekan
Tiger sharks were sampled off the western (Ningaloo Reef, Shark Bay) and eastern (the Great Barrier Reef; GBR, Queensland and New South Wales; NSW) coastlines of Australia. Multiple tissues were collected from each shark to investigate the effects of location, size and sex of sharks on δ(13)C and δ(15)N stable isotopes among these locations. Isotopic composition of sharks sampled in reef and seagrass habitats (Shark Bay, GBR) reflected seagrass-based food-webs, whereas at Ningaloo Reef analysis revealed a dietary transition between pelagic and seagrass food-webs...
August 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28794272/identifying-the-right-mentor
#18
Luciana Catanese, Ashkan Shoamanesh
No abstract text is available yet for this article.
August 9, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28793118/quality-of-life-of-elderly-people-with-chronic-kidney-disease-in-conservative-treatment
#19
Roberta Maria de Pina Pereira, Marcelo Aparecido Batista, Aline de Sousa Meira, Marília Pilotto de Oliveira, Luciana Kusumota
Objective: To describe the quality of life (QOL) of elderly people with Chronic Kidney Disease (CKD) in conservative treatment, correlating it with sociodemographic and health-related aspects. Method: This is a quantitative, cross-sectional, and descriptive study that used: a previously validated instrument for data collection; the WHOQOL-BREF and WHOQOL-OLD QOL scales; and the Mini-Mental State Examination. Results: Thirty-five elderly people (54...
July 2017: Revista Brasileira de Enfermagem
https://www.readbyqxmd.com/read/28793111/nursing-diagnosis-in-older-adults-with-chronic-kidney-disease-on-hemodialysis
#20
Mayara Cristina Debone, Elisângela da Silva Nunes Pedruncci, Maristela do Carmo Peterossi Candido, Sueli Marques, Luciana Kusumota
Objective: To identify the main nursing diagnoses (NSs) in older adult patients under hemodialysis treatment. Method: Exploratory research using case studies in data collection performed by interview and physical examination of older adults, in the first semester of 2016. Were included twenty-eight older adults undergoing chronic hemodialysis treatment who met the selection criteria. The analysis followed two steps (RISNER, 1990): Phase I - Data analysis and synthesis; and Phase II - Establishment of nursing diagnoses using the taxonomy of NANDA-I (2015)...
July 2017: Revista Brasileira de Enfermagem
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