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Di Wu, Guoyuan Liu, Yufeng Liu, Hexige Saiyin, Chenji Wang, Zhen Wei, Wenjiao Zen, Danyang Liu, Qi Chen, Zhonghua Zhao, Liping Zou, Haojie Huang, Songmin Jiang, Long Yu
UNLABELLED: Interplay between cell polarity module Scribble-Lethal Giant Larvae-Discs Large 1 (DLG1) and Yes-associated protein (YAP) appears critical in tumor metastasis. We identified zinc finger protein 191 (ZNF191) as a metastasis suppressor acting through DLG-YAP crosstalk in hepatocellular carcinoma (HCC). Overexpression of ZNF191 in HCC cells impaired cell motility, while ZNF191 depletion promoted cell migration in vitro and metastasis in vivo through triggering YAP signaling. Chromatin immunoprecipitation-sequencing revealed that ZNF191 specifically bound to the promoter of DLG1, a cell polarity maintainer and a negative regulator of YAP...
October 2016: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Chinta Someswara Rao, S Viswanadha Raju
Next generation sequencing (NGS) technologies have been rapidly applied in biomedical and biological research in recent years. To provide the comprehensive NGS resource for the research, in this paper , we have considered 10 loci/codi/repeats TAGA, TCAT, GAAT, AGAT, AGAA, GATA, TATC, CTTT, TCTG and TCTA. Then we developed the NGS Tandem Repeat Database (TandemRepeatDB) for all the chromosomes of Homo sapiens, Callithrix jacchus, Chlorocebus sabaeus, Gorilla gorilla, Macaca fascicularis, Macaca mulatta, Nomascus leucogenys, Pan troglodytes, Papio anubis and Pongo abelii genome data sets for all those locis...
March 2016: Genomics Data
Ying-Shi Guan, Yunke Qin, Yuanhui Sun, Jie Chen, Wei Xu, Daoben Zhu
Single-bundle nanofiber based OFETs were fabricated from co-assembled supramolecular nanofibers which comprise TCAT and PDI-13, using a simple gelation method. The co-assembled supramolecular nanoarchitecture was fully characterized by means of optical microscopy, TEM, SEM, 2D-GIWAXS and so on. The devices exhibited typical ambipolar charge transport characteristics with very well-balanced hole and electron mobilities as well as high photoresponsivity under ambient conditions.
March 28, 2016: Chemical Communications: Chem Comm
Honghan Fei, Seth M Cohen
The incorporation of 2,3-dimercaptoterephthalate (thiocatecholate, tcat) into a highly robust UiO-type metal-organic framework (MOF) has been achieved via postsynthetic exchange (PSE). The anionic, electron-donating thiocatecholato motif provides an excellent platform to obtain site-isolated and coordinatively unsaturated soft metal sites in a robust MOF architecture. Metalation of the thiocatechol group with palladium affords unprecedented Pd-mono(thiocatecholato) moieties within these MOFs. Importantly, Pd-metalated MOFs are efficient, heterogeneous, and recyclable catalysts for regioselective functionalization of sp(2) C-H bond...
February 18, 2015: Journal of the American Chemical Society
G Sciumè, S Shelton, Wg Gray, Ct Miller, F Hussain, M Ferrari, P Decuzzi, Ba Schrefler
Several mathematical formulations have analyzed the time-dependent behaviour of a tumor mass. However, most of these propose simplifications that compromise the physical soundness of the model. Here, multiphase porous media mechanics is extended to model tumor evolution, using governing equations obtained via the Thermodynamically Constrained Averaging Theory (TCAT). A tumor mass is treated as a multiphase medium composed of an extracellular matrix (ECM); tumor cells (TC), which may become necrotic depending on the nutrient concentration and tumor phase pressure; healthy cells (HC); and an interstitial fluid (IF) for the transport of nutrients...
January 2013: New Journal of Physics
Fengrui Li, Jinfeng Xuan, Jiaxin Xing, Mei Ding, Baojie Wang, Hao Pang
Several commercial multiplex PCR kits for the amplification of short tandem repeat (STR) loci have been extensively applied in forensic genetics. Consequently, large numbers of samples have been genotyped, and the number of discordant genotypes observed has also increased. We observed allele dropout with two novel alleles at the STR loci TH01 and D13S317 during paternity testing using the AmpFℓSTR Identifiler PCR Amplification Kit. The lost alleles reappeared when alternative PCR primer pairs were used. A sequence analysis revealed a G-to-A substitution 82 bases downstream of the last TCAT motif of the repeat region at the TH01 locus (GenBank accession: D00269) and a G-to-T substitution 90 bases upstream of the first TATC motif of the repeat region at the D13S317 locus (GenBank accession: G09017)...
January 2014: Forensic Science International. Genetics
Jiayi Hu, Lai Fong Chan, Renan P Souza, Maria Tampakeras, James L Kennedy, Clement Zai, Vincenzo De Luca
Evidence has shown that attempted suicide in psychiatric disorders is a complex interplay of genes and environment. Noradrenergic dysfunction due to abnormalities in the tyrosine hydroxylase (TH) gene has been implicated in the pathogenesis of suicidal behavior in mood disorders. However, suicide is a leading cause of mortality in schizophrenia too. Recent evidence suggests that TH gene variants may also increase the risk of suicide attempts in schizophrenia patients, although the interaction with established clinical risk factors is unclear...
January 24, 2014: Neuroscience Letters
Tae-Lim Yoon, Kyung-Mi Park, Sil-Ah Choi, Ji-Hyun Lee, Hyo-Jung Jeong, Heon-Seock Cynn
A wide range of intra- and inter-rater reliabilities of the trochanteric prominence angle test (TPAT) has been reported. We introduced the transcondylar angle test (TCAT) as an alternative to the TPAT and using a smartphone as a reliable measurement tool for femoral neck anteversion (FNA) measurement. The reliabilities of the TPAT and the TCAT, the reliability of using a smartphone as a clinical measurement tool, and the correlation between the difference value of medial knee joint space (KJS) between rest and tested positions and the difference value between the TPAT and TCAT were assessed...
April 2014: Manual Therapy
Mateo I Sánchez, Olalla Vázquez, M Eugenio Vázquez, José L Mascareñas
Transcription factors (TFs) are specialized proteins that play a key role in the regulation of genetic expression. Their mechanism of action involves the interaction with specific DNA sequences, which usually takes place through specialized domains of the protein. However, achieving an efficient binding usually requires the presence of the full protein. This is the case for bZIP and zinc finger TF families, which cannot interact with their target sites when the DNA binding fragments are presented as isolated monomers...
July 22, 2013: Chemistry: a European Journal
Jesús Mosquera, Adrián Jiménez-Balsa, Verónica I Dodero, M Eugenio Vázquez, José L Mascareñas
One of the strategies used by nature to regulate gene expression relies on the stimuli-controlled combination of DNA-binding proteins. This in turn determines the target-binding site within the genome, and thereby whether a particular gene is activated or repressed. Here we demonstrate how a designed basic region leucine zipper-based peptide can be directed towards two different DNA sequences depending on its dimerization arrangement. While the monomeric peptide is non-functional, a C-terminal metallo-dimer recognizes the natural ATF/CREB-binding site (5'-ATGA cg TCAT-3'), and a N-terminal disulphide dimer binds preferentially to the swapped sequence (5'-TCAT cg ATGA-3')...
2013: Nature Communications
Jenna van Draanen, Simon Corneau, Thomas Henderson, Adam Quastel, Robin Griller, Vicky Stergiopoulos
The Toronto Community Addictions Team (TCAT) is an intensive case management intervention designed to serve people with addictions who are frequent service users, thus addressing a health system priority. Questionnaires given to 65 participants at baseline, 3 months, and 6 months and semi-structured interviews of 10 program participants explored participants' outcomes and experiences with the program. Qualitative findings, analyzed using thematic content analysis, suggest that participants value the program's commitment to harm reduction, financial trusteeship, and recovery orientation...
May 2013: Substance Use & Misuse
G Sciumè, S E Shelton, W G Gray, C T Millers, F Hussain, M Ferrari, P Decuzzi, B A Schrefler
Multiphase porous media mechanics is used for modeling tumor growth, using governing equations obtained via the thermodynamically constrained averaging theory (TCAT). This approach incorporates the interaction of more phases than legacy tumor growth models. The tumor is treated as a multiphase system composed of an extracellular matrix, tumor cells which may become necrotic depending on nutrient level and pressure, healthy cells and an interstitial fluid which transports nutrients. The governing equations are numerically solved within a Finite Element framework for predicting the growth rate of the tumor mass, and of its individual components, as a function of the initial tumor-to-healthy cell ratio, nutrient concentration, and mechanical strain...
September 2012: Molecular & Cellular Biomechanics: MCB
Donald S Shepard, Wu Zeng, Gail K Strickler, Aung K Lwin, Marion J Cros, Bryan R Garner
Although several costing instruments have been previously developed, few have been validated or applied systematically to the delivery of evidence-based practices (EBPs). Using data collected from 26 organizations implementing the same EBP, this paper examined the reliability, validity, and applicability of the brief Treatment Cost Analysis Tool (TCAT-Lite). The TCAT-Lite demonstrated good reliability-correlations between replications averaged 0.61. Validity also was high, with correlation of treated episodes per $100,000 between the TCAT-Lite and independent data of 0...
October 1, 2012: Alcoholism Treatment Quarterly
William G Gray, Cass T Miller
This work is the fifth in a series of papers on the thermodynamically constrained averaging theory (TCAT) approach for modeling flow and transport phenomena in multiscale porous medium systems. The general TCAT framework and the mathematical foundation presented in previous works are used to develop models that describe species transport and single-fluid-phase flow through a porous medium system in varying physical regimes. Classical irreversible thermodynamics formulations for species in fluids, solids, and interfaces are developed...
May 1, 2009: Advances in Water Resources
Garima Singh, Sekar Ramachandran, Richard A Cerione
The activation of Gα subunits of heterotrimeric G proteins by G protein-coupled receptors (GPCRs) is a critical event underlying a variety of biological responses. Understanding how G proteins are activated will require structural and biochemical analyses of GPCRs complexed to their G protein partners, together with structure-function studies of Gα mutants that shed light on the different steps in the activation pathway. Previously, we reported that the substitution of a glycine for a proline at position 56 within the linker region connecting the helical and GTP-binding domains of a Gα chimera, designated αT*, yields a more readily exchangeable state for guanine nucleotides...
April 17, 2012: Biochemistry
Ajay Abraham, Savitha Varatharajan, Salar Abbas, Wei Zhang, Ramachandran V Shaji, Rayaz Ahmed, Aby Abraham, Biju George, Alok Srivastava, Mammen Chandy, Vikram Mathews, Poonkuzhali Balasubramanian
AIM: Cytidine deaminase (CDA) irreversibly deaminates cytarabine (Ara-C), a key component of acute myeloid leukemia (AML) induction and consolidation therapy. CDA overexpression results in Ara-C resistance, while decreased expression is associated with toxicity. We evaluated factors influencing variation in CDA mRNA expression in adult AML patients and normal controls, and how they contributed to Ara-C cytotoxicity in AML cells. MATERIALS & METHODS: CDA mRNA expression in 100 de novo AML patients and 36 normal controls were determined using quantitative reverse-transcriptase PCR...
February 2012: Pharmacogenomics
Guoyuan Liu, Songmin Jiang, Chenji Wang, Wei Jiang, Zulong Liu, Chao Liu, Hexige Saiyin, Xianmei Yang, Suqin Shen, Deke Jiang, Ping Zhou, Dingding Han, Xiaohui Hu, Qing Yi, Long Yu
UNLABELLED: Activation of β-catenin, the central effector of the canonical wingless-type (Wnt) pathway, has been implicated in hepatocellular carcinoma (HCC). However, the transcription regulation mechanism of the β-catenin gene in HCC remains unknown. Here we report that human zinc finger protein 191 (ZNF191) is a potential regulator of β-catenin transcription. ZNF191, a Krüppel-like protein, specifically interacts with the TCAT motif, which constitutes the HUMTH01 microsatellite in the tyrosine hydroxylase (TH) gene ex vivo...
June 2012: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Monique C D van der Burg-Koorevaar, Silvia Miret, Guus S M J E Duchateau
The aim of this study was to investigate whether milk reduces the bioaccessibility of tea catechins, which would compromise tea beneficial effects ascribed to polyphenols. Adding milk to black tea has been shown to lead to polyphenol-protein complexes. So far, data on the intestinal stability of polyphenol-protein complexes are scarce. English black tea (0.93 ± 0.06 mol/L total catechins) and Indian black tea (1.83 ± 0.08 mol/L catechins) were prepared with skimmed or full-fat milk and subjected to simulated gastric, small intestinal, and brush border digestion...
July 27, 2011: Journal of Agricultural and Food Chemistry
William G Gray, Cass T Miller
Standard models of flow of two immiscible fluids in a porous medium make use of an expression for the dependence of capillary pressure on the saturation of a fluid phase. Data to support the mathematical expression is most often obtained through a sequence of equilibrium experiments. In addition to such expressions being hysteretic, recent experimental and theoretical studies have suggested that the equilibrium functional forms obtained may be inadequate for modeling dynamic systems. This situation has led to efforts to express relaxation of a system to an equilibrium capillary pressure in relation to the rate of change of saturation...
June 2011: Advances in Water Resources
William G Gray, Cass T Miller
This work is the eighth in a series that develops the fundamental aspects of the thermodynamically constrained averaging theory (TCAT) that allows for a systematic increase in the scale at which multiphase transport phenomena is modeled in porous medium systems. In these systems, the explicit locations of interfaces between phases and common curves, where three or more interfaces meet, are not considered at scales above the microscale. Rather, the densities of these quantities arise as areas per volume or length per volume...
December 1, 2010: Advances in Water Resources
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