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https://www.readbyqxmd.com/read/29665895/-inhibitory-effect-of-mir-125b-down-regulation-on-proliferation-of-leukemia-cell-k562
#1
Jie Liu, Chang-Qing Tong
OBJECTIVE: To explore inhibitory effect of miR-125b down-regulation on proliferation of leukemia cell K562. METHODS: miR-125b inhibitor and miR-125b NC were transfected into K562 cells by liposome LipofectamineTM 2000, the cell viability was measured by MTT assay, cell cloning ability was detected by agarose cloning assay, cell cycle was measured by flow cytometry. The expression of BCL-2, BCL-2 homology antagonist/liller 1(BAK1), p53 and p53 up-regulated modulator of apoptosis (Puma) was measured by Western blot...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/29382425/-knockdown-of-dna-pkcs-inhibits-cell-cycle-and-its-mechanism-of-drug-resistant-bel7402-5-fu-hepatocellular-carcinoma-cells
#2
Dayu Li, Yun Liu, Chunbo Yu, Xiping Liu, Fang Fan
Objective To study the effect of the knock-down of the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) on the cell cycle of the multidrug-resistant (MDR) Bel7402/5-Fu hepatocellular carcinoma cells and its MDR mechanism. Methods After cationic liposome-mediated siDNA-PKcs oligonucleotide transfection, the drug sensitivity of Bel7402/5-Fu cells to 5-fluorouracil (5-Fu) and adriamycin (ADM) was determined by MTT assay; the cell cycle were detected by flow cytometry; meanwhile, the protein expressions of cell cycle-related proteins P21, cell cycle protein B1 (cyclin B1), cell cycle division protein 2 (CDC2) were tested by Western blotting; the expressions of ataxia telangiectasia mutated (ATM) and p53 at both mRNA and protein levels were detected by real-time PCR and Western blot analysis...
December 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29377650/photodynamic-therapy-with-liposomal-zinc-phthalocyanine-and-tirapazamine-increases-tumor-cell-death-via-dna-damage
#3
Mans Broekgaarden, Ruud Weijer, Alebert C van Wijk, Ruud C Cox, Maarten R Egmond, Ron Hoebe, Thomas M van Gulik, Michal Heger
The efficacy of photodynamic therapy (PDT) in some solid tumors is limited by the poor biodistributive properties of conventional photosensitizers and a natural predisposition of tumor cells to survive hypoxia and oxidative stress. This study investigated the therapeutic potential of a third-generation photosensitizer, liposomal zinc phthalocyanine (ZnPC), in combination with the hypoxic cytotoxin tirapazamine (TPZ). TPZ induces DNA double strand breaks (DSBs) under hypoxic conditions and subsequent apoptosis via p53 signaling...
February 2017: Journal of Biomedical Nanotechnology
https://www.readbyqxmd.com/read/29376759/estrogen-receptor-mediated-liposomal-drug-delivery-for-treating-melanoma
#4
Anirban Ganguly, Hari Krishna Reddy Rachamalla, Dwaipayan Bhattacharya, Keerti Bhamidipati, Abhishek Pal, Halley Gora Ravuri, Sumana Chakrabarti, Susanta Sekhar Adhikari, Rajkumar Banerjee
Function of steroid hormone estrogen that transactivates estrogen receptor (ER) is expressed in multiple organs. Except for malignancies of gynecological organs, ER remains largely unutilized as a target to treat cancers of ER-expressing brain, prostate, skin etc. We have previously developed estrogen targeting cationic lipid molecule (ES-C10), which showed targeted killing of ER+ breast and skin cancer cells. In this study, we explored the targeting ability of ES-C10 as a ligand as well as its additive killing effect (if any), when incorporated in two different liposomes (DCME and DCDE), carrying two anticancer molecules MCIS3 and DocetaxelTM respectively...
January 29, 2018: Journal of Drug Targeting
https://www.readbyqxmd.com/read/29346317/liposomal-tricurin-a-synergistic-combination-of-curcumin-epicatechin-gallate-and-resveratrol-repolarizes-tumor-associated-microglia-macrophages-and-eliminates-glioblastoma-gbm-and-gbm-stem-cells
#5
Sumit Mukherjee, Juliet N E Baidoo, Samay Sampat, Andrew Mancuso, Lovena David, Leah S Cohen, Shuiqin Zhou, Probal Banerjee
Glioblastoma (GBM) is a deadly brain tumor with a current mean survival of 12-15 months. Despite being a potent anti-cancer agent, the turmeric ingredient curcumin (C) has limited anti-tumor efficacy in vivo due to its low bioavailability. We have reported earlier a strategy involving the use two other polyphenols, epicatechin gallate (E) from green tea and resveratrol (R) from red grapes at a unique, synergistic molar ratio with C (C:E:R: 4:1:12.5, termed TriCurin) to achieve superior potency against HPV+ tumors than C alone at C:E:R (μM): 32:8:100 (termed 32 μM+ TriCurin)...
January 18, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29126924/suppression-of-p53r2-gene-expression-with-specific-sirna-sensitizes-hepg2-cells-to-doxorubicin
#6
Ako Azimi, Maryam Majidinia, Vahid Shafiei-Irannejad, Rana Jahanban-Esfahlan, Yasin Ahmadi, Ansar Karimian, Seyed Mostafa Mir, Hadi Karami, Bahman Yousefi
INTRODUCTION: p53R2 is a p53-inducible protein that contributes to DNA repair by providing dNTPs in response to DNA damage. The roles of p53R2 in cancer cells and malignancies still remain controversial. Herein, we examined the effects of p53R2 silencing on HepG2 human hepatocellular carcinoma (HHC) cell line (wild-type p53) viability, apoptosis and cell cycle arrest in the presence and absence of doxorubicin. METHODS: Cell transfection was performed using a liposomal approach...
February 5, 2018: Gene
https://www.readbyqxmd.com/read/29078269/ko-of-5-insp-7-kinase-activity-transforms-the-hct116-colon-cancer-cell-line-into-a-hypermetabolic-growth-inhibited-phenotype
#7
Chunfang Gu, Hoai-Nghia Nguyen, Douglas Ganini, Zhaowei Chen, Henning J Jessen, Zhen Gu, Huanchen Wang, Stephen B Shears
The inositol pyrophosphates 5-InsP7 (diphosphoinositol pentakisphosphate) and 1,5-InsP8 (bis-diphosphoinositol tetrakisphosphate) are highly energetic cellular signals interconverted by the diphosphoinositol pentakisphosphate kinases (PPIP5Ks). Here, we used CRISPR to KO PPIP5Ks in the HCT116 colon cancer cell line. This procedure eliminates 1,5-InsP8 and raises 5-InsP7 levels threefold. Expression of p53 and p21 was up-regulated; proliferation and G1/S cell-cycle transition slowed. Thus, PPIP5Ks are potential targets for tumor therapy...
November 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28976121/strategies-used-in-the-clinical-trials-of-gene-therapy-for-cancer
#8
Thekkuttuparambil Ananthanarayanan Ajith
Advances in understanding and manipulating genes have set the stage for scientists to alter a person's genetic material to prevent or treat diseases. Over the past decade, somatic gene therapy has been increasingly applied in clinical trials where the genetic material (DNA and RNA) introduced into a person's cell. Mutation and inactivation of the tumor suppressor genes are the unified concept of the development of tumor in humans. Therefore, researchers have discovered potential of gene therapies in the treatment of cancer...
September 2017: Journal of Experimental Therapeutics & Oncology
https://www.readbyqxmd.com/read/28932113/cationic-lipid-based-nanoparticles-mediate-functional-delivery-of-acetate-to-tumor-cells-in-vivo-leading-to-significant-anticancer-effects
#9
Leigh P Brody, Meliz Sahuri-Arisoylu, James R Parkinson, Harry G Parkes, Po Wah So, Nabil Hajji, E Louise Thomas, Gary S Frost, Andrew D Miller, Jimmy D Bell
Metabolic reengineering using nanoparticle delivery represents an innovative therapeutic approach to normalizing the deregulation of cellular metabolism underlying many diseases, including cancer. Here, we demonstrated a unique and novel application to the treatment of malignancy using a short-chain fatty acid (SCFA)-encapsulated lipid-based delivery system - liposome-encapsulated acetate nanoparticles for cancer applications (LITA-CAN). We assessed chronic in vivo administration of our nanoparticle in three separate murine models of colorectal cancer...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28916339/d-amino-acid-mutation-of-pmi-as-potent-dual-peptide-inhibitors-of-p53-mdm2-mdmx-interactions
#10
Xiang Li, Chao Liu, Si Chen, Honggang Hu, Jiacan Su, Yan Zou
According to the previously reported potent dual l-peptide PMI of p53-MDM2/MDMX interactions, a series of d-amino acid mutational PMI analogues, PMI-1-4, with enhanced proteolytic resistence and in vitro tumor cell inhibitory activities were reported, of which Liposome-PMI-1 showed a stronger inhibitory activity against the U87 cell lines than Nutlin-3. This d-amino acid mutation strategy may give a hand for enhancing the potential of peptide drugs.
October 15, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28912139/a-combination-rnai-chemotherapy-layer-by-layer-nanoparticle-for-systemic-targeting-of-kras-p53-with-cisplatin-to-treat-non-small-cell-lung-cancer
#11
Li Gu, Zhou J Deng, Sweta Roy, Paula T Hammond
Purpose: Mutation of the Kirsten ras sarcoma viral oncogene homolog (KRAS) and loss of p53 function are commonly seen in patients with non-small cell lung cancer (NSCLC). Combining therapeutics targeting these tumor-defensive pathways with cisplatin in a single-nanoparticle platform are rarely developed in clinic. Experimental Design: Cisplatin was encapsulated in liposomes, which multiple polyelectrolyte layers, including siKRAS and miR-34a were built on to generate multifunctional layer-by-layer nanoparticle...
December 1, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28891579/sensitive-and-selective-detection-of-the-p53-gene-based-on-a-triple-helix-magnetic-probe-coupled-to-a-fluorescent-liposome-hybridization-assembly-via-rolling-circle-amplification
#12
Xia Li, Juan Song, Qingwang Xue, Haiyan Zhao, Min Liu, Baoli Chen, Yun Liu, Wei Jiang, Chen-Zhong Li
Developing a sensitive and selective sensing platform for the p53 gene and its mutation analysis is essential and may aid in early cancer screening and assessment of prognosis. Here, we developed a highly sensitive and selective p53 gene assay based on the coupling of a triple-helix magnetic probe (THMP) to a fluorescent liposome hybridization assembly, a process initiated by rolling circle amplification (RCA). In the presence of p53, the THMP unfolds and activates an enzymatic cleavage reaction, thus releasing the RCA primer and initiating the RCA product-assisted fluorescent liposome hybridization assembly...
October 7, 2017: Analyst
https://www.readbyqxmd.com/read/28735042/improved-selectivity-and-cytotoxic-effects-of-irinotecan-via-liposomal-delivery-a-comparative-study-on-hs68-and-hela-cells
#13
Ana Casadó, Margarita Mora, Maria Lluïsa Sagristá, Santi Rello-Varona, Pilar Acedo, Juan Carlos Stockert, Magdalena Cañete, Angeles Villanueva
Irinotecan (CPT-11) is an effective chemotherapeutic agent widely used to treat different cancers. Otherwise, the liposomal delivery of anti-tumor agents has been shown to be a promising strategy. The aim of this study has been to analyze the effect of liposomal CPT-11 (CPT-11lip) on two human cell lines (Hs68 and HeLa) to establish the suitability of this CPT-11 nanocarrier. We have demonstrated the highest uptake of CPT-11lip in comparison with that of CPT-11sol, in lactate buffer, and that CPT-11lip was internalized in the cells through an endocytic process whereas CPT-11sol does so by passive diffusion...
July 19, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28700899/pegylated-liposomal-formulation-of-doxorubicin-overcomes-drug-resistance-in-a-genetically-engineered-mouse-model-of-breast-cancer
#14
András Füredi, Kornélia Szebényi, Szilárd Tóth, Mihály Cserepes, Lilla Hámori, Veronika Nagy, Edina Karai, Péter Vajdovich, Tímea Imre, Pál Szabó, Dávid Szüts, József Tóvári, Gergely Szakács
Success of cancer treatment is often hampered by the emergence of multidrug resistance (MDR) mediated by P-glycoprotein (ABCB1/Pgp). Doxorubicin (DOX) is recognized by Pgp and therefore it can induce therapy resistance in breast cancer patients. In this study our aim was to evaluate the susceptibility of the pegylated liposomal formulation of doxorubicin (PLD/Doxil®/Caelyx®) to MDR. We show that cells selected to be resistant to DOX are cross-resistant to PLD and PLD is also ineffective in an allograft model of doxorubicin-resistant mouse B-cell leukemia...
September 10, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28627280/the-targeting-effect-of-hm2e8b-nctd-liposomes-on-b-lineage-leukaemia-stem-cells-is-associated-with-the-hlf-slug-axis
#15
Jingying Zhang, Diying Shen, Min Jia, Haizhao Zhao, Yongmin Tang
To identify an agent with specific activity against B-lineage leukaemia stem cells (B-LSCs), we generated norcantharidin (NCTD)-encapsulated liposomes modified with a novel humanised anti-human CD19 monoclonal antibody, Hm2E8b (Hm2E8b-NCTD-liposomes). These liposomes were specially designed to recognise and kill B-LSCs in vitro, and to decrease non-specific cytotoxicity to untargeted cells. Hm2E8b-NCTD-liposomes selectively ablated B-LSCs through targeting hepatic leukaemia factor (HLF), which is implicated in haematopoietic stem cell regulation and is overexpressed in LSCs...
January 2018: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28624193/mir-375-and-doxorubicin-co-delivered-by-liposomes-for-combination-therapy-of-hepatocellular-carcinoma
#16
Yin-Ping Fan, Jia-Zhi Liao, Ya-Qi Lu, De-An Tian, Feng Ye, Peng-Xuan Zhao, Guang-Ya Xiang, Wang-Xian Tang, Xing-Xing He
Doxorubicin (DOX) is one of the most frequently used anti-cancer drugs and the front line option for hepatocellular carcinoma (HCC) treatment. However, the clinical applications of DOX are restricted largely due to its toxicity and chemoresistance. Here, we report that miR-375 and DOX were co-delivered by liposomes (named L-miR-375/DOX-NPs) for combination therapy of HCC and drug resistance reversion of DOX. In vitro, L-miR-375/DOX-NPs could deliver DOX and miR-375 efficiently and simultaneously into HCC cells and ensure the successful release of mature miR-375 and DOX...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28565974/pnc27-anticancer-peptide-as-targeting-ligand-significantly-improved-antitumor-efficacy-of-doxil-in-hdm2-expressing-cells
#17
Shahrzad Amiri Darban, Ali Badiee, Mahmoud Reza Jaafari
AIM: To investigate the potential of PNC27 peptide, 12-26 of p53 with high affinity for HDM2 protein, as targeting ligand for Doxil to improve its antitumor activity. MATERIALS & METHODS: Doxil postinserted with 25, 50, 100 and 200 PNC27 peptides per liposome. Flow cytometry and confocal analysis were performed on C26 colon carcinoma (HDM2 positive) and B16F0 melanoma (HDM2 negative) cells. In vivo studies were performed on BALB/c mice bearing C26 and C57BL/6 mice bearing B16F0 tumor models...
June 1, 2017: Nanomedicine
https://www.readbyqxmd.com/read/28503689/effects-of-elemene-on-inhibiting-proliferation-of-vascular-smooth-muscle-cells-and-promoting-reendothelialization-at-the-stent-implantation-site
#18
Wenjie Sun, Yuhua Huang, Tieying Yin, Jingjing Wang, Ruolin Du, Juhui Qiu, Yuan Zhang, Yazhou Wang, Jinju Chen, Guixue Wang
Anticancer drugs are commonly used as inhibitors of vascular smooth muscle cell (VSMC) proliferation in clinical treatments. This study aims to investigate how elemene affects the proliferation of VSMCs, the restenosis, and the reendothelialization after implanting the elemene-coated stents. VSMCs derived from rat aorta were used to test the cell proliferation, cell cycle, migration, apoptosis, cytoskeletal protein F-actin, intracellular Ca(2+), IncRNA chip and gene expression of PCNA, P53, and Cx43 when cultured with elemene...
May 30, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28400564/a-novel-4-arm-dna-rna-nanoconstruct-triggering-rapid-apoptosis-of-triple-negative-breast-cancer-cells-within-24%C3%A2-hours
#19
Joline Tung, Lih Shin Tew, Yuan-Man Hsu, Yit Lung Khung
Measuring at ~30 nm, a fully customizable holliday junction DNA nanoconstruct, was designed to simultaneously carry three unmodified SiRNA strands for apoptosis gene knockout in cancer cells without any assistance from commercial transfection kits. In brief, a holliday junction structure was intelligently designed to present one arm with a cell targeting aptamer (AS1411) while the remaining three arms to carry different SiRNA strands by means of DNA/RNA duplex for inducing apoptosis in cancer cells. By carrying the three SiRNA strands (AKT, MDM2 and Survivin) into triple negative breast MDA-MB-231 cancer cells, cell number had reduced by up to ~82% within 24 hours solely from one single administration of 32 picomoles...
April 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28351626/integrated-immunochromatographic-strip-with-glucometer-readout-for-rapid-quantification-of-phosphorylated-proteins
#20
Yuting Zhao, Xiao Chen, Sophie Lin, Dan Du, Yuehe Lin
A new technology to quantify phospho-p53(15) by converting its content to the amount of glucose which is detectable by a glucometer was developed. An immunochromatographic test strip (ITS) was used as a disposable platform, where primary antibody (Ab1)-modified Fe3O4 magnetic nanoparticles (Fe3O4-Ab1) were settled on the test zone to capture both the target phospho-p53(15) and the detection antibody (Ab2)-glucose encapsulating liposome (GEL) conjugate. The measurement was based on the release and subsequent detection of glucose from Ab2-GEL using a glucose meter (GM)...
April 29, 2017: Analytica Chimica Acta
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