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Microbiology laboratory automation

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https://www.readbyqxmd.com/read/28412378/project-management-importance-for-diagnostic-laboratories
#1
REVIEW
Antony Croxatto, Gilbert Greub
BACKGROUND: The constraints applied on diagnostic laboratories to improve both quality and productivity together with personal shortages incite laboratory managers to constantly optimize the laboratory workflows, organization and technology. These continuous modifications of the laboratories should be conducted using efficient project and change management approaches in order to maximize the opportunities to achieve the project successfully. AIM: This review aims at presenting a general overview of project management with an emphasis on selected critical aspects...
April 12, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28397472/comparison-of-novel-blood-culture-system-dl-bt112tm-with-bact-alert-3dtm-by-using-clinical-samples
#2
Mustafa Altindis, Tayfur Demiray, Mehmet Koroglu, Umit Kilic, Aybala Neslihan Alagoz, Aziz Ogutlu, Oguz Karabay
BACKGROUND: Blood cultures are the main diagnostic laboratory tool to detect bloodstream infections. Many clinical microbiology laboratories utilize automated blood culture systems to isolate infectious agents from blood samples. The diagnostic performance and time-to-detection values of the novel automated blood culture system, DLBt112TM (DL), was compared with BacT/Alert 3DTM (B3D) in this prospective comparative study with clinical samples. METHODS: A total of 356 blood culture sets (178 sets for each system) were evaluated over a 6-month period in a university hospital...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28391814/antibiogramj-a-tool-for-analysing-images-from-disk-diffusion-tests
#3
C A Alonso, C Domínguez, J Heras, E Mata, V Pascual, C Torres, M Zarazaga
BACKGROUND AND OBJECTIVES: Disk diffusion testing, known as antibiogram, is widely applied in microbiology to determine the antimicrobial susceptibility of microorganisms. The measurement of the diameter of the zone of growth inhibition of microorganisms around the antimicrobial disks in the antibiogram is frequently performed manually by specialists using a ruler. This is a time-consuming and error-prone task that might be simplified using automated or semi-automated inhibition zone readers...
May 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28366154/-evaluation-of-mycobacterial-microscopy-and-culture-results-of-sureyyapasa-chest-diseases-and-chest-surgery-training-and-research-hospital-a-3-year-analysis
#4
Elçin Akduman Alaşehir, Ahmet Balıkçı, Mualla Partal, Gülay Çatmabacak, Görkem Yaman
Introduction: Effective diagnosis of tuberculosis is of great importance for transmission control and treatment success. The purpose of this study is to evaluate microscopic examination results of Ehrlich-Ziehl Neelsen (EZN) and Auramine-Rhodamine staining methods and automated BACTEC MGIT 960™ system and Löwenstein-Jensen (L-J) culture results of various clinical samples in the light of recent data from the world and Turkey. Materials and Methods: Specimens that were sent from various clinics to Sureyyapasa Chest Diseases and Chest Surgery Training and Research Hospital Microbiology Laboratory from January 2012 to December 2015 were evaluated retrospectively...
September 2016: Tüberküloz Ve Toraks
https://www.readbyqxmd.com/read/28322712/implementation-and-initial-analysis-of-a-laboratory-based-weekly-biosurveillance-system-provence-alpes-c%C3%A3-te-d-azur-france
#5
Michael Huart, Gabriel Bedubourg, Cédric Abat, Philippe Colson, Jean Marc Rolain, Hervé Chaudet, Pierre Edouard Fournier, Didier Raoult, Xavier Deparis
We describe the implementation of an automated infectious disease surveillance system that uses data collected from 210 microbiologic laboratories throughout the Provence-Alpes-Côte d'Azur region in France. Each week, these facilities report bacterial species that have been isolated from patients in their area. An alarm is triggered whenever the case count for a bacterial species infection exceeds 2 SDs of the historical mean for that species at the participating laboratory. At its inception in July 2013, the system monitored 611 bacterial species...
April 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28274291/an-audit-of-cryptosporidium-and-giardia-detection-in-scottish-national-health-service-diagnostic-microbiology-laboratories
#6
C L Alexander, S Currie, K Pollock, A Smith-Palmer, B L Jones
Giardia duodenalis and Cryptosporidium species are protozoan parasites capable of causing gastrointestinal disease in humans and animals through the ingestion of infective faeces. Whereas Cryptosporidium species can be acquired locally or through foreign travel, there is the mis-conception that giardiasis is considered to be largely travel-associated, which results in differences in laboratory testing algorithms. In order to determine the level of variation in testing criteria and detection methods between diagnostic laboratories for both pathogens across Scotland, an audit was performed...
March 9, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28271567/the-welsh-blood-service-70-years-of-continuous-change
#7
REVIEW
G D Poole
The National Blood Transfusion Service (NBTS) in England and Wales was established as a single entity in 1946 and operated as such for almost half a century. During those 50 years, the blood service in Wales, as in the rest of the UK, saw many technological and operational changes. The automation of donation testing, the introduction of successive layers of microbiological screening, the creation of the Tissue Typing Laboratory (later renamed the Welsh Transplantation and Immunogenetics Laboratory) and the development of information technology brought - over a relatively long period - highly significant improvements to an organisation that had begun life as an Emergency Medical Service...
March 8, 2017: Transfusion Medicine
https://www.readbyqxmd.com/read/28214393/flow-cytometric-bacterial-cell-counts-challenge-conventional-heterotrophic-plate-counts-for-routine-microbiological-drinking-water-monitoring
#8
REVIEW
S Van Nevel, S Koetzsch, C R Proctor, M D Besmer, E I Prest, J S Vrouwenvelder, A Knezev, N Boon, F Hammes
Drinking water utilities and researchers continue to rely on the century-old heterotrophic plate counts (HPC) method for routine assessment of general microbiological water quality. Bacterial cell counting with flow cytometry (FCM) is one of a number of alternative methods that challenge this status quo and provide an opportunity for improved water quality monitoring. After more than a decade of application in drinking water research, FCM methodology is optimised and established for routine application, supported by a considerable amount of data from multiple full-scale studies...
April 15, 2017: Water Research
https://www.readbyqxmd.com/read/28164570/time-to-detection-comparison-for-a-novel-blood-culture-system-using-simulated-blood-cultures-dltm-versus-bact-alerttm-and-bactectm
#9
Tayfur Demiray, Mehmet Koroglu, Mustafa Altindis
BACKGROUND: Automated blood culture systems are routinely used in microbiology laboratories to isolate bacteria and fungi causing bloodstream infections. A novel automated blood culture system, DL-Bt112TM (DL) was compared with the BACTEC 9050TM (BCT) and BacT/Alert 3DTM (B3D) systems for time-to-detection (TTD) using 10 different clinical bacteria that commonly cause bloodstream infections. METHODS: Simulated blood cultures were used to compare the three automated blood culture systems...
September 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28129707/fluorescence-in-situ-hybridization-fish-in-the-microbiological-diagnostic-routine-laboratory-a-review
#10
REVIEW
Hagen Frickmann, Andreas Erich Zautner, Annette Moter, Judith Kikhney, Ralf Matthias Hagen, Henrik Stender, Sven Poppert
Early identification of microbial pathogens is essential for rational and conservative antibiotic use especially in the case of known regional resistance patterns. Here, we describe fluorescence in situ hybridization (FISH) as one of the rapid methods for easy identification of microbial pathogens, and its advantages and disadvantages for the diagnosis of pathogens in human infections in the laboratory diagnostic routine. Binding of short fluorescence-labeled DNA or nucleic acid-mimicking PNA probes to ribosomes of infectious agents with consecutive analysis by fluorescence microscopy allows identification of bacterial and eukaryotic pathogens at genus or species level...
May 2017: Critical Reviews in Microbiology
https://www.readbyqxmd.com/read/28124968/-accurate-diagnosis-of-pseudomonas-luteola-in-routine-microbiology-laboratory-on-the-occasion-of-two-isolates
#11
Muharrem Çiçek, Gülşen Hasçelik, H Kaan Müştak, K Serdar Diker, Burçin Şener
Pseudomonas luteola which was previously known as Chryseomonas luteola; is a gram-negative, non-fermentative, aerobic, motile, non-spore-forming bacillus. It is frequently found as a saprophyte in soil, water and other damp environments and is an opportunistic pathogen in patients with underlying medical disorders or with indwelling catheters. It has been reported as an uncommon cause of bacteremia, sepsis, septic arthritis, meningitis, endocarditis, and peritonitis. Thus, early and accurate identification of this rare species is important for the treatment and also to provide information about the epidemiology of P...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28122803/a-decade-of-development-of-chromogenic-culture-media-for-clinical-microbiology-in-an-era-of-molecular-diagnostics
#12
REVIEW
John D Perry
In the last 25 years, chromogenic culture media have found widespread application in diagnostic clinical microbiology. In the last decade, the range of media available to clinical laboratories has expanded greatly, allowing specific detection of additional pathogens, including Pseudomonas aeruginosa, group B streptococci, Clostridium difficile, Campylobacter spp., and Yersinia enterocolitica. New media have also been developed to screen for pathogens with acquired antimicrobial resistance, including vancomycin-resistant enterococci, carbapenem-resistant Acinetobacter spp...
April 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28087144/-contribution-of-microbiology-in-the-diagnosis-of-tuberculosis-in-castile-and-le%C3%A3-n-spain-findings-of-the-grumicale-2013-study
#13
Ramiro López-Medrano, Teresa Nebreda-Mayoral, M Fé Brezmes-Valdivieso, Susana García-de Cruz, Begoña Nogueira-González, Rafael Sánchez-Arroyo, Almudena Tinajas-Puertas, Nieves Gutiérrez-Zufiaurre, Cristina Labayru-Echeverría, Susana Hernando-Real, Luis López-Urrutia, Octavio Rivero-Lezcano, Belén Ullivarri-Francia, Raquel Rodríguez-Tarazona, Isabel Antolín-Ayala
INTRODUCTION AND OBJECTIVES: A retrospective study was conducted by collecting microbiological tuberculosis (TB) data in Castile and León during the year 2013 in order to determine the incidence and distribution of TB, and resistance to the tuberculostatic drug, and compare them with the epidemiological data provided by the Department of Epidemiological Surveillance (SIVE). MATERIAL AND METHODS: Microbiologists of the 14 hospitals of the Castile and León public health network (GRUMICALE) collected epidemiological, microbiological, and management data from the Microbiology laboratories in the community during the year 2013...
January 10, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28053214/the-bactec-fx-blood-culture-system-detects-brucella-melitensis-bacteremia-in-adult-patients-within-the-routine-1-week-incubation-period
#14
Moshe Sagi, Lior Nesher, Pablo Yagupsky
The performance of the Bactec FX blood culture system for detecting Brucella bacteremia within the routine 1-week incubation period was assessed in a prospective study conducted in an area in southern Israel in which Brucella melitensis is endemic. Aerobic vials (BD Bactec Plus Aerobic/F medium) inoculated with blood specimens obtained from adult patients with positive Rose-Bengal screening test results were monitored for 4 consecutive weeks, and blind subcultures of negative vials were performed on solid media on days 7 and 28...
March 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28043600/benefits-and-challenges-of-molecular-diagnostics-for-childhood-tuberculosis
#15
Cristina Gutierrez
Expanding tuberculosis (TB)-diagnostic services, including access to rapid tests, is a World Health Organization (WHO) strategy to accelerate progress toward ending TB. Faster and more sensitive molecular tests capable of diagnosing TB and drug-resistant TB have the technical capacity to address limitations associated with smears and cultures by increasing accuracy and shortening turnaround times as compared with those of these conventional laboratory methods. Nucleic acid amplification assays used to detect and analyze Mycobacterium tuberculosis (MTB)-complex nucleic acids can be used directly on specimens from patients suspected of having TB...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/27723450/fully-automated-clinical-grade-bone-marrow-processing-a-single-centre-experience
#16
Benedetta Mazzanti, Serena Urbani, Simone Dal Pozzo, Paola Bufano, Lara Ballerini, Alessia Gelli, Irene Sodi, Irene Donnini, Massimo Di Gioia, Stefano Guidi, Julien Camisani, Riccardo Saccardi
BACKGROUND: Clinical grade processing of harvested bone marrow is required in various clinical situations, particularly in the management of ABO mismatching in allogeneic haematopoietic stem cell transplantation (HSCT) and in regenerative medicine. MATERIAL AND METHODS: We report a single-centre experience using a fully automated, clinical grade, closed system (Sepax, Biosafe, Switzerland). From 2003 to 2015, 125 procedures were performed in our laboratory, including buffy-coat production for HSCT (n=58), regenerative medicine in an orthopaedic setting (n=54) and density-gradient separation in a trial for treatment of critical limb ischaemia (n=13)...
September 27, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27697104/validation-of-the-applied-biosystems-rapidfinder-shiga-toxin-producing-e-coli-stec-detection-workflow
#17
Jonathan Cloke, Sharon Matheny, Michelle Swimley, Robert Tebbs, Angelia Burrell, Jonathan Flannery, Benjamin Bastin, Patrick Bird, M Joseph Benzinger, Erin Crowley, James Agin, David Goins, Yvonne Salfinger, Michael Brodsky, Maria Cristina Fernandez
The Applied Biosystems™ RapidFinder™ STEC Detection Workflow (Thermo Fisher Scientific) is a complete protocol for the rapid qualitative detection of Escherichia coli (E. coli) O157:H7 and the "Big 6" non-O157 Shiga-like toxin-producing E. coli (STEC) serotypes (defined as serogroups: O26, O45, O103, O111, O121, and O145). The RapidFinder STEC Detection Workflow makes use of either the automated preparation of PCR-ready DNA using the Applied Biosystems PrepSEQ™ Nucleic Acid Extraction Kit in conjunction with the Applied Biosystems MagMAX™ Express 96-well magnetic particle processor or the Applied Biosystems PrepSEQ Rapid Spin kit for manual preparation of PCR-ready DNA...
November 1, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/27683527/interpretation-of-blood-microbiology-results-function-of-the-clinical-microbiologist
#18
Katalin Kristóf, Júlia Pongrácz
The proper use and interpretation of blood microbiology results may be one of the most challenging and one of the most important functions of clinical microbiology laboratories. Effective implementation of this function requires careful consideration of specimen collection and processing, pathogen detection techniques, and prompt and precise reporting of identification and susceptibility results. The responsibility of the treating physician is proper formulation of the analytical request and to provide the laboratory with complete and precise patient information, which are inevitable prerequisites of a proper testing and interpretation...
April 2016: EJIFCC
https://www.readbyqxmd.com/read/27650515/methicillin-resistant-staphylococcus-aureus-isolates-with-sccmec-type-v-and-spa-types-t437-or-t1081-associated-to-discordant-susceptibility-results-between-oxacillin-and-cefoxitin-central-taiwan
#19
Cheng-Mao Ho, Chien-Yu Lin, Mao-Wang Ho, Hsiao-Chuan Lin, Chao-Jung Chen, Lee-Chung Lin, Jang-Jih Lu
Staphylococcus aureus isolates with discordant susceptibility results between oxacillin and cefoxitin obtained using automated microbiology systems are infrequently observed. From April 2013 to December 2014, 1956 methicillin-resistant S. aureus (MRSA) and 1761 methicillin-susceptible S. aureus isolates were obtained from different patients. Forty isolates (1.1% and 2% in case of S. aureus and MRSA, respectively) with discordant susceptibility results (oxacillin susceptible and cefoxitin resistant) and carrying mecA gene were obtained...
December 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27630804/emerging-bacterial-infection-identification-and-clinical-significance-of-kocuria-species
#20
REVIEW
Venkataramana Kandi, Padmavali Palange, Ritu Vaish, Adnan Bashir Bhatti, Vinod Kale, Maheshwar Reddy Kandi, Mohan Rao Bhoomagiri
Recently there have been reports of gram-positive cocci which are morphologically similar to both Staphylococci and the Micrococci. These bacteria have been identified as Kocuria species with the help of automated identification system and other molecular methods including 16S rRNA (ribosomal ribonucleic acid) evaluation. Kocuria belongs to the family Micrococcaceae which also includes Staphylococcus species and Micrococcus species. Isolation and clinical significance of these bacteria from human specimens warrant great caution as it does not necessarily confirm infection due to their ubiquitous presence, and as a normal flora of skin and mucous membranes in human and animals...
August 10, 2016: Curēus
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