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Microbiology laboratory automation

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https://www.readbyqxmd.com/read/29765558/the-fallopian-tube-microbiome-implications-for-reproductive-health
#1
Elise S Pelzer, Dana Willner, Melissa Buttini, Louise M Hafner, Christina Theodoropoulos, Flavia Huygens
Objective: There is a paucity of data characterizing the microbiota of the female upper genital tract, which controversially is described as a sterile site. We examine whether the fallopian tube harbours an endogenous microbial community. Design: This prospective study collected from women undergoing total hysterectomy or salpingectomy-oophorectomy. Setting: Private hospital gynaecology department. Patients: Fallopian tubes were collected from women diagnosed with benign disease or for prophylaxis...
April 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29738325/automated-low-flow-ascites-pump-in-a-real-world-setting-complications-and-outcomes
#2
Philipp Solbach, Christoph Höner Zu Siederdissen, Freya Wellhöner, Nicolas Richter, Benjamin Heidrich, Henrike Lenzen, Port Kerstin, Katja Hueper, Michael P Manns, Heiner Wedemeyer, Elmar Jaeckel
BACKGROUND AND AIMS: The aim of this study was to investigate the Alfapump, an automated low-flow pump system for the treatment of refractory ascites (RA) as an alternative for repeated large-volume paracentesis in patients with contraindication for placement of a transjugular intrahepatic portosystemic shunt (TIPS) or liver transplantation. MATERIALS AND METHODS: In 21 consecutive patients with RA and contraindication for a placement of a TIPS, the Alfapump was implanted at Hannover Medical School between December 2012 and May 2016...
May 7, 2018: European Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/29651616/significant-increase-in-cultivation-of-gardnerella-vaginalis-alloscardovia-omnicolens-actinotignum-schaalii-and-actinomyces-spp-in-urine-samples-with-total-laboratory-automation
#3
Sabrina Klein, Dennis Nurjadi, Susanne Horner, Klaus Heeg, Stefan Zimmermann, Irene Burckhardt
While total laboratory automation (TLA) is well established in laboratory medicine, only a few microbiological laboratories are using TLA systems. Especially in terms of speed and accuracy, working with TLA is expected to be superior to conventional microbiology. We compared in total 35,564 microbiological urine cultures with and without incubation and processing with BD Kiestra TLA for a 6-month period each retrospectively. Sixteen thousand three hundred thirty-eight urine samples were analyzed in the pre-TLA period and 19,226 with TLA...
April 13, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29642831/-identification-of-anaerobic-gram-negative-bacilli-isolated-from-various-clinical-specimens-and-determination-of-antibiotic-resistance-profiles-with-e-test-methods
#4
Cengiz Demir, Recep Keşli
The aim of this study was to identify gram-negative anaerobic bacilli isolated from various clinical specimens that were obtained from patients with suspected anaerobic infections and to determine the antibiotic resistance profiles by using the antibiotic concentration gradient method. The study was performed in Afyon Kocatepe University Ahmet Necdet Sezer Research and Practice Hospital, Medical Microbiology Laboratory between 1 November 2014 and 30 October 2015. Two hundred and seventyeight clinical specimens accepted for anaerobic culture were enrolled in the study...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29642825/-investigation-of-carbapenemase-genes-and-molecular-epidemiology-of-enterobacteriaceae-strains-isolated-between-2010-2014-in-a-university-hospitals
#5
Hüseyin Haydar Kutlu, Ebru Us, Alper Tekeli
The worldwide spread of carbapenemase producing Enterobacteriaceae isolates has become a major threat of public health. This worrisome situation leads the development of new methods for carbapenemase screening, detection, prevention of spread and epidemiological data collection as mandatory. In this study, it was aimed to investigate existence and distribution of carbapenemase-encoding genes (CEGs) among carbapenem-resistant Enterobacteriaceae isolated from various clinical samples in Ankara University Faculty of Medicine, Ibni Sina Hospital, Central Microbiology Laboratory between June 2010-May 2014 and detect their clonal relationship...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29581314/total-laboratory-automation-in-clinical-microbiology-a-micro-comic-strip
#6
EDITORIAL
Alexander J McAdam
No abstract text is available yet for this article.
April 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29573441/contemporary-microbiology-and-identification-of-corynebacteria-spp-causing-infections-in-human
#7
REVIEW
Aleksandra Anna Zasada, Ewa Mosiej
The Corynebacterium genus are bacteria of growing clinical importance. It is because of progress in medicine that result in growing population of immunocompromised patients and growing number of infections caused by opportunistic pathogens. A new infections caused by Corynebacterium species regarded previously as commensal microorganisms as well as infections caused by new Corynebacterium species are described. Parallel with changes in Corynebacteria infections the microbiological laboratory diagnostic possibilities are changing...
March 24, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29541448/multidrug-resistant-gram-negative-bacterial-infections-in-a-teaching-hospital-in-ghana
#8
Nicholas Agyepong, Usha Govinden, Alex Owusu-Ofori, Sabiha Yusuf Essack
Background: Multidrug-resistant Gram-negative bacteria have emerged as major clinical and therapeutic dilemma in hospitals in Ghana.To describe the prevalence and profile of infections attributable to multidrug-resistant Gram-negative bacteria among patients at the Komfo Anokye Teaching Hospital in the Ashanti region of Ghana. Methods: Bacterial cultures were randomly selected from the microbiology laboratory from February to August, 2015. Bacterial identification and minimum inhibitory concentrations were conducted using standard microbiological techniques and the Vitek-2 automated system...
2018: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29522576/rapid-and-easy-detection-of-low-level-resistance-to-vancomycin-in-methicillin-resistant-staphylococcus-aureus-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#9
Kota Asakura, Takuya Azechi, Hiroshi Sasano, Hidehito Matsui, Hideaki Hanaki, Motoyasu Miyazaki, Tohru Takata, Miwa Sekine, Tomoiku Takaku, Tomonori Ochiai, Norio Komatsu, Keigo Shibayama, Yuki Katayama, Koji Yahara
Vancomycin-intermediately resistant Staphylococcus aureus (VISA) and heterogeneous VISA (hVISA) are associated with treatment failure. hVISA contains only a subpopulation of cells with increased minimal inhibitory concentrations, and its detection is problematic because it is classified as vancomycin-susceptible by standard susceptibility testing and the gold-standard method for its detection is impractical in clinical microbiology laboratories. Recently, a research group developed a machine-learning classifier to distinguish VISA and hVISA from vancomycin-susceptible S...
2018: PloS One
https://www.readbyqxmd.com/read/29479855/experimental-fusion-of-different-versions-of-the-total-laboratory-automation-system-and-improvement-of-laboratory-turnaround-time
#10
Hee-Jung Chung, Yoon Kyung Song, Sang-Hyun Hwang, Do Hoon Lee, Tetsuro Sugiura
BACKGROUND: Use of total laboratory automation (TLA) system has expanded to microbiology and hemostasis and upgraded to second and third generations. We herein report the first successful upgrades and fusion of different versions of the TLA system, thus improving laboratory turnaround time (TAT). METHODS: A 21-day schedule was planned from the time of pre-meeting to installation and clinical sample application. We analyzed the monthly TAT in each menu, distribution of the "out of range for acceptable TAT" samples, and "prolonged time out of acceptable TAT," before and after the upgrade and fusion...
February 25, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29474660/performance-of-the-accelerate-pheno%C3%A2-system-for-identification-and-antimicrobial-susceptibility-testing-of-a-panel-of-multidrug-resistant-gram-negative-bacilli-directly-from-positive-blood-cultures
#11
Alix Pantel, Julien Monier, Jean-Philippe Lavigne
Objectives: To evaluate the performance of the Accelerate Pheno™ system for the identification and antimicrobial susceptibility testing (AST) of a panel of Gram-negative bacilli (GNB) with different resistance profiles (e.g. penicillinases, ESBLs, cephalosporinase overproduction, carbapenemases, impermeability) directly from positive blood cultures in <7 h. Methods: A panel of 105 clinical strains previously characterized for the presence of β-lactamase-encoding genes was tested...
February 20, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29458156/how-to-isolate-identify-and-determine-antimicrobial-susceptibility-of-anaerobic-bacteria-in-routine-laboratories
#12
REVIEW
E Nagy, L Boyanova, U S Justesen
BACKGROUND: There has been increased interest in the study of anaerobic bacteria that cause human infection during the past decade. Many new genera and species have been described using 16S rRNA gene sequencing of clinical isolates obtained from different infection sites with commercially available special culture media to support the growth of anaerobes. Several systems, such as anaerobic pouches, boxes, jars and chambers provide suitable anaerobic culture conditions to isolate even strict anaerobic bacteria successfully from clinical specimens...
February 17, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29433835/towards-automated-detection-semi-quantification-and-identification-of-microbial-growth-in-clinical-bacteriology-a-proof-of-concept
#13
Antony Croxatto, Raphaël Marcelpoil, Cédrick Orny, Didier Morel, Guy Prod'hom, Gilbert Greub
BACKGROUND: Automation in microbiology laboratories impacts management, workflow, productivity and quality. Further improvements will be driven by the development of intelligent image analysis allowing automated detection of microbial growth, release of sterile samples, identification and quantification of bacterial colonies and reading of AST disk diffusion assays. We investigated the potential benefit of intelligent imaging analysis by developing algorithms allowing automated detection, semi-quantification and identification of bacterial colonies...
December 2017: Biomedical Journal
https://www.readbyqxmd.com/read/29428064/automatic-hemolysis-identification-on-aligned-dual-lighting-images-of-cultured-blood-agar-plates
#14
Mattia Savardi, Alessandro Ferrari, Alberto Signoroni
BACKGROUND AND OBJECTIVE: The recent introduction of Full Laboratory Automation systems in clinical microbiology opens to the availability of streams of high definition images representing bacteria culturing plates. This creates new opportunities to support diagnostic decisions through image analysis and interpretation solutions, with an expected high impact on the efficiency of the laboratory workflow and related quality implications. Starting from images acquired under different illumination settings (top-light and back-light), the objective of this work is to design and evaluate a method for the detection and classification of diagnostically relevant hemolysis effects associated with specific bacteria growing on blood agar plates...
March 2018: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/29426791/collection-transport-and-general-processing-of-clinical-specimens-in-microbiology-laboratory
#15
M Isabel Sánchez-Romero, Juan Manuel García-Lechuz Moya, Juan José González López, Nieves Orta Mira
The interpretation and the accuracy of the microbiological results still depend to a great extent on the quality of the samples and their processing within the Microbiology laboratory. The type of specimen, the appropriate time to obtain the sample, the way of sampling, the storage and transport are critical points in the diagnostic process. The availability of new laboratory techniques for unusual pathogens, makes necessary the review and update of all the steps involved in the processing of the samples. Nowadays, the laboratory automation and the availability of rapid techniques allow the precision and turn-around time necessary to help the clinicians in the decision making...
February 6, 2018: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29411645/application-of-the-maldi-biotyper-to-clinical-microbiology-progress-and-potential
#16
Markus Kostrzewa
The introduction of the MALDI Biotyper in laboratories substantially changed microbiology practice, this has been called a revolution. The system accelerated diagnostic while costs were reduced and accuracy was increased. In just a few years MALDI-TOF MS became the first-line identification tool for microorganisms. Ten years after its introduction, more than 2000 MALDI Biotyper systems are installed in laboratories which are performing routine diagnostic, and the number is still increasing. Areas covered: This article summarises changes in clinical microbiology introduced by the MALDI Biotyper and its effects, as it has been published in peer reviewed articles found in PubMed...
March 2018: Expert Review of Proteomics
https://www.readbyqxmd.com/read/29366760/rapid-and-cost-effective-identification-and-antimicrobial-susceptibility-testing-in-patients-with-gram-negative-bacteremia-directly-from-blood-culture-fluid
#17
Christina Sakarikou, Anna Altieri, Maria Cristina Bossa, Silvia Minelli, Camilla Dolfa, Micol Piperno, Cartesio Favalli
Rapid pathogen identification (ID) and antimicrobial susceptibility testing (AST) in bacteremia cases or sepsis could improve patient prognosis. Thus, it is important to provide timely reports, which make it possible for clinicians to set up appropriate antibiotic therapy during the early stages of bloodstream infection (BSI). This study evaluates an in-house microbiological protocol for early ID as well as AST on Gram negative bacteria directly from positive monomicrobial and polymicrobial blood cultures (BCs)...
March 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29314254/manual-versus-automated-streaking-system-in-clinical-microbiology-laboratory-performance-evaluation-of-previ-isola-for-blood-culture-and-body-fluid-samples
#18
Qute Choi, Hyun Jin Kim, Jong Wan Kim, Gye Cheol Kwon, Sun Hoe Koo
BACKGROUND: The process of plate streaking has been automated to improve routine workflow of clinical microbiology laboratories. Although there were many evaluation reports about the inoculation of various body fluid samples, few evaluations have been reported for blood. In this study, we evaluated the performance of automated inoculating system, Previ Isola for various routine clinical samples including blood. METHODS: Blood culture, body fluid, and urine samples were collected...
January 4, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29310696/methods-for-detecting-gemmata-spp-bacteremia-in-the-microbiology-laboratory
#19
Jacques-Robert Christen, Edwin Edmond, Michel Drancourt
OBJECTIVE: Gemmata bacteria are fastidious, Gram-negative and aerobic. The only representatives are Gemmata obscuriglobus and Gemmata massiliana. These Planctomycetes appear to be a part of human digestive tract microbiome, and G. massiliana has been isolated from water. Further specific detection in the blood of two patients with febrile neutropenia suggests that Gemmata bacteremia may remain under-documented. The objective of this study was to develop an effective protocol to document Gemmata spp...
January 8, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29287094/validation-of-a-novel-automatic-deposition-of-bacteria-and-yeasts-on-maldi-target-for-maldi-tof-ms-based-identification-using-maldi-colonyst-robot
#20
Katerina Chudejova, Michal Bohac, Anna Skalova, Veronika Rotova, Costas C Papagiannitsis, Jana Hanzlickova, Tamara Bergerova, Jaroslav Hrabák
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) -based identification of bacteria and fungi significantly changed the diagnostic process in clinical microbiology. We describe here a novel technique for bacterial and yeast deposition on MALDI target using an automated workflow resulting in an increase of the microbes' score of MALDI identification. We also provide a comparison of four different sample preparation methods. In the first step of the study, 100 Gram-negative bacteria, 100 Gram-positive bacteria, 20 anaerobic bacteria and 20 yeasts were spotted on the MALDI target using manual deposition, semi-extraction, wet deposition onto 70% formic acid and by automatic deposition using MALDI Colonyst...
2017: PloS One
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