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https://www.readbyqxmd.com/read/28550594/comparison-of-rapid-diagnostic-tests-to-detect-mycobacterium-avium-subsp-paratuberculosis-disseminated-infection-in-bovine-liver
#1
Mehdi Zarei, Masoud Ghorbanpour, Samaneh Tajbakhsh, Nader Mosavari
Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne's disease, a chronic enteritis in cattle and other domestic and wild ruminants. The presence of MAP in tissues other than intestines and associated lymph nodes, such as meat and liver, is a potential public health concern. In the present study, the relationship between the results of rapid diagnostic tests of the Johne's disease, such as serum ELISA, rectal scraping PCR, and acid-fast staining, and the presence of MAP in liver was evaluated. Blood, liver, and rectal scraping samples were collected from 200 slaughtered cattle with unknown Johne's disease status...
May 27, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28550299/microfluidic-isolation-of-circulating-tumor-cell-clusters-by-size-and-asymmetry
#2
Sam H Au, Jon Edd, Amy E Stoddard, Keith H K Wong, Fabio Fachin, Shyamala Maheswaran, Daniel A Haber, Shannon L Stott, Ravi Kapur, Mehmet Toner
Circulating tumor cell clusters (CTC clusters) are potent initiators of metastasis and potentially useful clinical markers for patients with cancer. Although there are numerous devices developed to isolate individual circulating tumor cells from blood, these devices are ineffective at capturing CTC clusters, incapable of separating clusters from single cells and/or cause cluster damage or dissociation during processing. The only device currently able to specifically isolate CTC clusters from single CTCs and blood cells relies on the batch immobilization of clusters onto micropillars which necessitates long residence times and causes damage to clusters during release...
May 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28549450/the-left-atrial-bacterial-vegetative-mass-due-to-corynebacterium-striatum-as-a-presentation-of-myxoma-a-case-report
#3
Jun Xu, Qing Yang, Jun Li, Xia Zheng
BACKGROUND: Corynebacterium striatum is a member of the non-diphtherial corynebacteria, which are ubiquitous in nature and generally colonize the skin and mucous membranes of humans. Rarely, it causes infective endocarditis (IE). We report a case of rare left atrial bacterial vegetative mass due to C. striatum masquerading as a myxoma identified through a tortuous diagnostic process, and present a brief review of the relevant literature. CASE PRESENTATION: We present a case of 63-year-old man who presented with progressively worsening dyspnea on exertion and lower leg edema, and was diagnosed with heart failure...
May 26, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28548628/endothelial-progenitor-cells-for-the-vascularization-of-engineered-tissues
#4
Erica Brown Peters
Self-assembled microvasculature from cocultures of endothelial cells and stromal cells have significantly advanced efforts to vascularize engineered tissues by enhancing perfusion rates <i>in vivo</i> and producing investigative platforms for microvascular morphogenesis <i>in vitro</i>. However, in order to clinically translate prevascularized constructs, the issue of endothelial cell source must be resolved. Endothelial progenitor cells (EPCs) can be noninvasively supplied from the recipient through adult peripheral and umbilical cord blood, as well as derived from induced pluripotent stem cells, alleviating antigenicity issues...
May 26, 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/28547158/impact-of-prompt-intervention-in-response-to-positive-blood-culture-results-during-weekends-by-collaboration-between-infectious-disease-specialists-and-microbiology-laboratory-staff
#5
M Tsuboi, K Hayakawa, K Mezaki, Y Katanami, K Yamamoto, S Kutsuna, N Takeshita, N Ohmagari
The purpose of this investigation was to elucidate the impact of prompt intervention for patients whose blood culture results became positive during weekends, as this is not standard care in some countries. A retrospective cohort study was conducted in a tertiary referral hospital. From June 2015, results of positive blood cultures became available during weekends. If infectious disease specialists identified cases of bacteremia on suboptimal antimicrobial coverage, they contacted the primary team for modification of antibiotic treatment...
May 25, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28546952/time-dependency-of-morphological-remodeling-of-endothelial-cells-in-response-to-substrate-stiffness
#6
Zahra Goli-Malekabadi, Mohammad Tafazzoli-Shadpour, Ali Tamayol, Ehsan Seyedjafari
Introduction: Substrate stiffness regulates cellular behavior as cells experience different stiffness values of tissues in the body. For example, endothelial cells (ECs) covering the inner layer of blood vessels are exposed to different stiffness values due to various pathologic and physiologic conditions. Despite numerous studies, cells by time span sense mechanical properties of the substrate, but the response is not well understood. We hypothesized that time is a major determinant influencing the behavior of cells seeded on substrates of varying stiffness...
2017: BioImpacts: BI
https://www.readbyqxmd.com/read/28546532/regulation-of-c-jun-n-terminal-protein-kinase-jnk-pathway-in-apoptosis-of-endothelial-outgrowth-cells-induced-by-asymmetric-dimethylarginine
#7
Fu-Qing Zhang, Wei Lu, Wen-Xiao Yuan, Xin Li
BACKGROUND Endothelial outgrowth cells (EOCs) are terminal endothelial progenitor cells (EPCs). Asymmetric dimethylarginine (ADMA) has been identified as a novel risk factor for cardiovascular diseases. Our aim in the present study was to investigate the effect of regulation of asymmetric dimethylarginine (ADMA) on EOCs apoptosis and to explore the underlining mechanisms of c-Jun N-terminal protein kinase (JNK) pathway in the process. MATERIAL AND METHODS EOCs were harvested from umbilical cord blood and obtained by using density gradient centrifugation and adhesive culture methods...
May 26, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28546029/gardnerella-vaginalis-bacteremia-associated-with-severe-acute-encephalopathy-in-a-young-female-patient
#8
Jacques Tankovic, Albertas Timinskas, Migle Janulaitiene, Milda Zilnyte, Jean-Luc Baudel, Eric Maury, Aurelija Zvirbliene, Milda Pleckaityte
Gardnerella vaginalis is a facultative anaerobic bacterium that inhabits the genitourinary tract of both healthy women and those with bacterial vaginosis. We report a case of G. vaginalis bacteremia associated with severe toxic encephalopathy in a young woman. Anaerobic blood cultures yielded pure growth of small gram-variable rods later identified as G. vaginalis by both rapid biochemical tests and 16S rRNA gene sequencing. The patient recovered after treatment with amoxicillin-clavulanate according to the in vitro susceptibility testing...
May 22, 2017: Anaerobe
https://www.readbyqxmd.com/read/28545413/development-of-an-lps-based-elisa-for-diagnosis-of-yersinia-enterocolitica-o-3-infections-in-danish-patients-a-follow-up-study
#9
Tine Dalby, Eva Rasmussen, Peter Schiellerup, Karen Angeliki Krogfelt
BACKGROUND: The bacterium Yersinia enterocolitica causes gastroenteritis in humans. The study aimed to develop a diagnostic enzyme-linked immunosorbent assay (ELISA) for detection of Yersinia enterocolitica O:3 LPS antibodies in sera from Danish patients with suspected Yersinia enterocolitica O:3 gastrointestinal infection. As a part of this, antibody decay profiles after culture confirmed Yersinia enteritis were studied. RESULTS: An ELISA using Yersinia enterocolitica O:3 LPS as the coating antigen was developed for measuring IgA, IgG and IgM specific antibodies...
May 25, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28545155/rapid-diagnostic-tests-for-typhoid-and-paratyphoid-enteric-fever
#10
REVIEW
Lalith Wijedoru, Sue Mallett, Christopher M Parry
BACKGROUND: Differentiating both typhoid (Salmonella Typhi) and paratyphoid (Salmonella Paratyphi A) infection from other causes of fever in endemic areas is a diagnostic challenge. Although commercial point-of-care rapid diagnostic tests (RDTs) for enteric fever are available as alternatives to the current reference standard test of blood or bone marrow culture, or to the widely used Widal Test, their diagnostic accuracy is unclear. If accurate, they could potentially replace blood culture as the World Health Organization (WHO)-recommended main diagnostic test for enteric fever...
May 26, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28545085/splicing-factor-sf3b1k700e-mutant-dysregulates-erythroid-differentiation-via-aberrant-alternative-splicing-of-transcription-factor-tal1
#11
Shuiling Jin, Hairui Su, Ngoc-Tung Tran, Jing Song, Sydney S Lu, Ying Li, Suming Huang, Omar Abdel-Wahab, Yanyan Liu, Xinyang Zhao
More than 60% of myeloid dysplasia syndrome (MDS) contains mutations in genes encoding for splicing factors such as SF3B1, U2AF, SRSF2 and ZRSR2. Mutations in SF3B1 are associated with 80% cases of refractory anemia with ring sideroblast (RARS), a subtype of MDS. SF3B1K700E is the most frequently mutated site among mutations on SF3B1. Yet the molecular mechanisms on how mutations of splicing factors lead to defective erythropoiesis are not clear. SF3B1K700E mutant binds to an RNA binding protein, RBM15, stronger than the wild type SF3B1 protein in co-immunoprecipitation assays...
2017: PloS One
https://www.readbyqxmd.com/read/28544957/zebrafish-as-a-visual-and-dynamic-model-to-study-the-transport-of-nanosized-drug-delivery-systems-across-the-biological-barriers
#12
Ye Li, Xiaoqing Miao, Tongkai Chen, Xiang Yi, Ruibing Wang, Haitao Zhao, Simon Ming-Yuen Lee, Xueqing Wang, Ying Zheng
With the wide application of nanotechnology to drug delivery systems, a simple, dynamic and visual in vivo model for high-throughput screening of novel formulations with fluorescence markers across biological barriers is desperately needed. In vitro cell culture models have been widely used, although they are far from a complimentary in vivo system. Mammalian animal models are common predictive models to study transport, but they are costly and time consuming. Zebrafish (Danio rerio), a small vertebrate model, have the potential to be developed as an "intermediate" model for quick evaluations...
May 10, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28544844/image-of-the-month-concomitant-tricuspid-and-mitral-native-valve-infective-endocarditis
#13
Su-Jin Jeong, Sang-Hoon Seol, Pil Sang Song
A 33-year-old immunocompetent man was admitted to the authors' hospital with a one-month duration of fevers, chills, and non-productive coughs, and suddenonset weakness and ischemic pain of both lower extremities. Physical examination revealed crepitating rales heard over bilateral lung fields. On auscultation, a grade 2~3/6 systolic murmur was heard over the leftlower sternal border and apical area. Transthoracic echocardiography revealed a mobile mass on the tricuspid valve compatible with vegetation, in addition to concomitant mitral valve vegetation (Video 1)...
January 2017: Journal of Heart Valve Disease
https://www.readbyqxmd.com/read/28544640/an-in%C3%A2-vitro-model-demonstrates-the-potential-of-neoplastic-human-germ-cells-to-influence-the-tumour-microenvironment
#14
B Klein, H-C Schuppe, M Bergmann, M P Hedger, B E Loveland, K L Loveland
Testicular germ cell tumours (TGCT) typically contain high numbers of infiltrating immune cells, yet the functional nature and consequences of interactions between GCNIS (germ cell neoplasia in situ) or seminoma cells and immune cells remain unknown. A co-culture model using the seminoma-derived TCam-2 cell line and peripheral blood mononuclear cells (PBMC, n = 7 healthy donors) was established to investigate how tumour and immune cells each contribute to the cytokine microenvironment associated with TGCT...
May 23, 2017: Andrology
https://www.readbyqxmd.com/read/28544462/biomimetic-macroporous-pcl-scaffolds-for-ex-vivo-expansion-of-cord-blood-derived-cd34-cells-with-feeder-cells-support
#15
Xiuwei Pan, Qiong Sun, Yuanhao Zhang, Haibo Cai, Yun Gao, Yongjia Shen, Weian Zhang
Ex vivo expansion of hematopoietic stem cells (HSCs) with most current methods can hardly satisfy clinical application requirement. While in vivo, HSCs efficiently self-renew in niche where they interact with 3D extracellular matrix and stromal cells. Therefore, co-cultures of CD34(+) cells and mesenchyme stem cells derived from human amniotic membrane (hAMSCs) on the basis of biomimetic macroporous three-dimensional (3D) poly(ε-caprolactone) (PCL) scaffolds are developed, where scaffolds and hAMSCs are applied to mimic structural and cellular microenvironment of HSCs...
May 24, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28544067/vitamin-d3-alters-the-expression-of-toll-like-receptors-in-peripheral-blood-mononuclear-cells-of-patients-with-systemic-lupus-erythematosus
#16
Esmaeil Yazdanpanah, Mahmoud Mahmoudi, Maryam Sahebari, Zahra Rezaieyazdi, Seyed-Alireza Esmaeili, Nafiseh Tabasi, Soheila Jaberi, Amirhossein Sahebkar, Maryam Rastin
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by production of inflammatory cytokines and autoreactive antibodies due to the loss of immune tolerance. Recognition of self-nucleic acids by intracellular Toll-like receptors (TLRs) can overactivate immune responses and this abnormal activation of TLRs contributes to the pathogenesis of the disease. In recent years, anti-inflammatory and immunomodulatory effects of 1, 25-dihydroxyvitamin D3 (VitD3) on the immune system has received particular attention...
May 20, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28543519/a-new-treatment-of-sepsis-caused-by-veillonella-parvula-a-case-report-and-literature-review
#17
J Li, P Chen, J Li, X Gao, X Chen, J Chen
WHAT IS KNOWN AND OBJECTIVES: Veillonella parvula is usually regarded as a contaminant bacteria associated with polymicrobial infection. Here we report a rare case of sepsis caused by V. parvula, which was successfully treated by chloramphenicol. In addition, the therapeutic strategy for V. parvula infection was reviewed. CASE SUMMARY: A 55-year-old woman underwent surgery for lumbar spinal stenosis and developed high fever (39.5°C) after surgery, accompanied by chills and fatigue...
May 23, 2017: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/28542841/blood-donors-perceptions-motivators-and-deterrents-in-sub-saharan-africa-a-scoping-review-of-evidence
#18
REVIEW
Lucy Asamoah-Akuoko, Oliver W Hassall, Imelda Bates, Henrik Ullum
Achieving an adequate blood supply in Sub-Saharan Africa (SSA) through donor mobilization and retention is crucial. Factors that motivate or deter blood donors vary according to beliefs and social norms. Understanding the factors that influence blood donation behaviour in SSA is vital to developing effective strategies to address blood donor motivation and retention. This review of 35 studies from 16 SSA countries collates available evidence concerning the perceptions, motivators and deterrents that influence blood donors in SSA...
May 22, 2017: British Journal of Haematology
https://www.readbyqxmd.com/read/28542614/evaluation-of-the-sepsis-flow-chip-assay-for-the-diagnosis-of-blood-infections
#19
Antonio Galiana, Javier Coy, Adelina Gimeno, Noemi Marco Guzman, Francisco Rosales, Esperanza Merino, Gloria Royo, Juan Carlos Rodríguez
BACKGROUND: Blood infections are serious complex conditions that generally require rapid diagnosis and treatment. The big challenge is to reduce the time necessary to make a diagnosis with current clinical microbiological methods so as to improve the treatment given to patients. METHODS: In this study, we assess for the first time the Sepsis Flow Chip assay, which is a novel diagnostic assay for simultaneous rapid-detection of the vast majority of bloodstream pathogens, including Gram-positive and Gram-negative bacteria and fungi, in the same assay, and for the detection of most common antibiotic resistance genes...
2017: PloS One
https://www.readbyqxmd.com/read/28542587/seminal-plasma-induces-inflammation-and-enhances-hiv-1-replication-in-human-cervical-tissue-explants
#20
Andrea Introini, Stéphanie Boström, Frideborg Bradley, Anna Gibbs, Axel Glaessgen, Annelie Tjernlund, Kristina Broliden
The most immediate and evident effect of mucosal exposure to semen in vivo is a local release of proinflammatory mediators accompanied by an influx of leukocytes into the female genital mucosa (FGM). The implication of such response in HIV-1 transmission has never been addressed due to limitations of currently available experimental models. Using human tissue explants from the uterine cervix, we developed a system of mucosal exposure to seminal plasma (SP) that supports HIV-1 replication. Treatment of ectocervical explants with SP resulted in the upregulation of inflammatory and growth factors, including IL-6, TNF, CCL5, CCL20, CXCL1, and CXCL8, and IL1A, CSF2, IL7, PTGS2, as evaluated by measuring protein levels in explant conditioned medium (ECM) and gene expression in tissue...
May 19, 2017: PLoS Pathogens
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