keyword
MENU ▼
Read by QxMD icon Read
search

Blood cultures

keyword
https://www.readbyqxmd.com/read/28742844/control-of-runx-induced-repression-of-notch-signaling-by-mlf-and-its-partner-dnaj-1-during-drosophila-hematopoiesis
#1
Marion Miller, Aichun Chen, Vanessa Gobert, Benoit Augé, Mathilde Beau, Odile Burlet-Schiltz, Marc Haenlin, Lucas Waltzer
A tight regulation of transcription factor activity is critical for proper development. For instance, modifications of RUNX transcription factors dosage are associated with several diseases, including hematopoietic malignancies. In Drosophila, Myeloid Leukemia Factor (MLF) has been shown to control blood cell development by stabilizing the RUNX transcription factor Lozenge (Lz). However, the mechanism of action of this conserved family of proteins involved in leukemia remains largely unknown. Here we further characterized MLF's mode of action in Drosophila blood cells using proteomic, transcriptomic and genetic approaches...
July 25, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28742513/kallistatin-double-edged-role-in-angiogenesis-apoptosis-and-oxidative-stress
#2
Julie Chao, Pengfei Li, Lee Chao
Kallistatin via its two structural elements - an active site and a heparin-binding domain - displays double-edged function in angiogenesis, apoptosis and oxidative stress. First, kallistatin has both anti-angiogenic and pro-angiogenic effects. Kallistatin treatment attenuates angiogenesis and tumor growth in cancer-bearing mice. Kallistatin via its heparinbinding site inhibits angiogenesis by blocking vascular endothelial growth factor (VEGF)- induced growth, migration and adhesion of endothelial cells. Conversely, kallistatin via the active site promotes neovascularization by stimulating VEGF levels in endothelial progenitor cells...
July 26, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/28742143/impact-of-rapid-identification-of-positive-blood-cultures-using-the-verigene-system-on-antibiotic-prescriptions-a-prospective-study-of-community-onset-bacteremia-in-a-tertiary-hospital-in-japan
#3
Kayoko Hayakawa, Kazuhisa Mezaki, Masao Kobayakawa, Kei Yamamoto, Yoshikazu Mutoh, Motoyuki Tsuboi, Takehiro Hasimoto, Maki Nagamatsu, Satoshi Kutsuna, Nozomi Takeshita, Yuichi Katanami, Masahiro Ishikane, Norio Ohmagari
BACKGROUND: Rapid identification of positive blood cultures is important for initiation of optimal treatment in septic patients. Effects of automated, microarray-based rapid identification systems on antibiotic prescription against community-onset bacteremia (COB) remain unclear. METHODS: We prospectively enrolled 177 patients with 185 COB episodes (occurring within 72 h of admission) over 17 months. Bacteremia episodes due to gram-positive bacteria (GP) and gram-negative bacteria (GN) in the same patient were counted separately...
2017: PloS One
https://www.readbyqxmd.com/read/28742076/altered-neurite-morphology-and-cholinergic-function-of-induced-pluripotent-stem-cell-derived-neurons-from-a-patient-with-kleefstra-syndrome-and-autism
#4
J Nagy, J Kobolák, S Berzsenyi, Z Ábrahám, H X Avci, I Bock, Z Bekes, B Hodoscsek, A Chandrasekaran, A Téglási, P Dezső, B Koványi, E T Vörös, L Fodor, T Szél, K Németh, A Balázs, A Dinnyés, B Lendvai, G Lévay, V Román
The aim of the present study was to establish an in vitro Kleefstra syndrome (KS) disease model using the human induced pluripotent stem cell (hiPSC) technology. Previously, an autism spectrum disorder (ASD) patient with Kleefstra syndrome (KS-ASD) carrying a deleterious premature termination codon mutation in the EHMT1 gene was identified. Patient specific hiPSCs generated from peripheral blood mononuclear cells of the KS-ASD patient were differentiated into post-mitotic cortical neurons. Lower levels of EHMT1 mRNA as well as protein expression were confirmed in these cells...
July 25, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/28741746/skin-lesion-in-a-patient-after-hematopoietic-stem-cell-transplant
#5
Erica E Kaufman West, Mudresh Mehta, Vinit Patel, Robin Chamberland
We present a case of a 61-year-old Caucasian woman who was hospitalized with fevers on day 176 after a matched unrelated stem cell transplant for acute myelogenous leukemia. She developed hemorrhagic bullae on the skin of her right thigh, and blood cultures and skin biopsy both confirmed Fusarium proliferatum. Despite antifungal therapy, her condition worsened and she died while on comfort-only measures. This article is protected by copyright. All rights reserved.
July 25, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28741656/biochemical-evaluation-of-storage-lesion-in-canine-packed-erythrocytes
#6
C R Wilson, M B Pashmakova, J A Heinz, M C Johnson, H M Minard, M A Bishop, J W Barr
OBJECTIVES: This study describes the biochemical changes - also known as the storage lesion - that occur in canine packed red blood cells during ex vivo storage. MATERIALS AND METHODS: Ten 125-mL units of non-leuco-reduced packed red blood cells in citrate phosphate dextrose adenine were obtained from a commercial blood bank within 24 hours of donation. Samples were aseptically collected on days 1, 4, 7, 14, 28, 35 and 42 for measurement of sodium, potassium, chloride, lactate, glucose, pH and ammonia concentrations...
July 25, 2017: Journal of Small Animal Practice
https://www.readbyqxmd.com/read/28741035/biomarkers-of-hepatic-injury-and-function-in-neonatal-hypoxic-ischemic-encephalopathy-and-with-therapeutic-hypothermia
#7
Hemananda Muniraman, Danielle Gardner, Jane Skinner, Anna Paweletz, Anitha Vayalakkad, Ying Hui Chee, Clare Clifford, Sunil Sanka, Vidheya Venkatesh, Anna Curley, Suresh Victor, Mark A Turner, Paul Clarke
Therapeutic hypothermia (TH) is now provided as standard care to infants with moderate-severe hypoxic ischemic encephalopathy (HIE). The role of TH in limiting neuronal injury is well recognized, but its effect on hepatic injury which occurs frequently in neonatal HIE is not known. Our objective was to characterize biomarkers of liver injury and function in the setting of neonatal HIE and to describe whether HIE severity and provision of TH influence these hepatic biomarkers. We performed a multicenter retrospective study and compared hepatic biomarkers obtained during the first postnatal week, according to the severity of HIE and whether treated with TH...
July 24, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28740980/micro-engineered-perfusable-3d-vasculatures-for-cardiovascular-diseases
#8
Nishanth Venugopal Menon, Hui Min Tay, Soon Nan Wee, King Ho Holden Li, Han Wei Hou
Vessel geometries in microengineered in vitro vascular models are important to recapitulate a pathophysiological microenvironment for the study of flow-induced endothelial dysfunction and inflammation in cardiovascular diseases. Herein, we present a simple and novel extracellular matrix (ECM) hydrogel patterning method to create perfusable vascularized microchannels of different geometries based on the concept of capillary burst valve (CBV). No surface modification is necessary and the method is suitable for different ECM types including collagen, matrigel and fibrin...
July 25, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28740836/mycotic-aneurysm-with-aortoduodenal-fistula
#9
REVIEW
Bruno Kusznir Vitturi, Amanda Frias, Raphael Sementilli, Marcelo de Castro Jorge Racy, Roberto Augusto Caffaro, Geanete Pozzan
Firstly described in the 19th century by Sir William Osler, the mycotic aneurysm (MA) is a rare entity characterized by an abnormal arterial dilation, which is potentially fatal, and is associated with the infection of the vascular wall. Elderly patients are mostly involved, especially when risk factors like chronic diseases, immunosuppression, neoplasia, and arterial manipulation are associated. The authors report the case of a young male patient diagnosed with an aortic aneurysm of infectious origin in the presence of repeated negative blood cultures...
April 2017: Autopsy & case reports
https://www.readbyqxmd.com/read/28740496/evaluation-of-antigen-specific-igm-and-igg-production-during-an-in-vitro-peripheral-blood-mononuclear-cell-culture-assay
#10
Yoshiko Matsuda, Ryoichi Imamura, Shiro Takahara
The recent attention given to diseases associated with memory B-cell (mBC)-produced antibodies (Abs) suggests the need for a similar in vitro assay to evaluate the functions of mBCs. Here, we cultured peripheral blood mononuclear cells (PBMCs) with the intent to collect mBC-derived Abs in vitro and maintain their cell-cell contact-dependent interactions with helper T-cells. PBMCs were cultured with interleukin (IL)-21, CpG-oligodeoxynucleotides (ODN), phorbol myristate acetate (PMA), and phytohemagglutinin/leucoagglutinin (PHA-L) in 24-well flat-bottom plates (5 × 10(5) cells/well)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28739652/in-hospital-quality-of-care-measures-for-pediatric-sepsis-syndrome
#11
Folafoluwa O Odetola, Gary Freed, Caroline Shevrin, Brian Madden, Julie McCormick, Kevin Dombkowski
BACKGROUND AND OBJECTIVES: Sepsis syndrome, comprising sepsis, severe sepsis, and septic shock, is a leading cause of child mortality and morbidity, for which the delivery of time-sensitive care leads to improved survival. We aimed to describe the development and testing of quality measures for in-hospital care of pediatric sepsis syndrome. METHODS: Seven measures of quality of care for children hospitalized with sepsis syndrome were developed by using an iterative process including literature review, development of concepts and candidate measures, and selection of measures for feasibility and importance by 2 panels of experts...
July 24, 2017: Pediatrics
https://www.readbyqxmd.com/read/28738848/influence-of-borderline-cefepime-mic-on-the-outcome-of-cefepime-susceptible-pseudomonas-aeruginosa-bacteremia-treated-with-a-maximal-cefepime-dose-a-hospital-based-retrospective-study
#12
Ting-Yi Su, Jung-Jr Ye, Chien-Chang Yang, Ching-Tai Huang, Ju-Hsin Chia, Ming-Hsun Lee
BACKGROUND: We assessed the influence of current cefepime minimal inhibitory concentration (MIC) breakpoints and the maximal cefepime dose on treatment outcomes in patients with bacteremia caused by cefepime-susceptible Pseudomonas aeruginosa. METHODS: Adult patients hospitalized between July 2010 and June 2014 with a positive blood culture for cefepime-susceptible P. aeruginosa and receipt of cefepime as the primary therapy throughout the course were reviewed. Cefepime Etest(®) MICs and clinical outcomes for P...
July 24, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28738837/assessment-of-cerebral-circulation-in-a-porcine-model-of-intravenously-given-e-coli-induced-fulminant-sepsis
#13
Levente Molnár, Norbert Németh, Mariann Berhés, Endre Hajdú, Lóránd Papp, Ábel Molnár, Judit Szabó, Ádám Deák, Béla Fülesdi
BACKGROUND: The aim of the present work was to assess cerebral hemodynamic changes in a porcine model of E.coli induced fulminant sepsis. METHODS: Nineteen healthy female Hungahib pigs, 10-12 weeks old, randomly assigned into two groups: Control (n = 9) or Septic Group (n = 10). In the Sepsis group Escherichia coli culture suspended in physiological saline was intravenously administrated in a continuously increasing manner according to the following protocol: 2 ml of bacterial culture suspended in physiological saline was injected in the first 30 min, then 4 ml of bacterial culture was administered within 30 min, followed by infusion of 32 ml bacterial culture for 2 h...
July 24, 2017: BMC Anesthesiology
https://www.readbyqxmd.com/read/28738709/nebulized-hypertonic-saline-to-prevent-ventilator-associated-pneumonia-in-premature-infants-a-randomized-trial
#14
Zahra Ezzeldin, Yasmeen Mansi, Mervat Gaber, Rania Zakaria, Rehab Fawzy, Mohamed A Mohamed
BACKGROUND/OBJECTIVES: Ventilator associated pneumonia (VAP) is a leading cause of death. Nebulized hypertonic saline solution (HSS) has been used to improve pulmonary clearance and reduce infection in intubated patients. This study examines whether nebulized HSS may reduce VAP in intubated premature infants. METHODS: We analyzed results of 100 intubated premature infants who completed the study. Infants were divided into the "Control" group where VAP prevention protocol was implemented and the "Intervention" group where nebulized HSS twice daily was added to the package of care...
July 24, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28738695/a-research-on-the-genotoxicity-of-stevia-in-human-lymphocytes
#15
Aslı Uçar, Serkan Yılmaz, Şemsigül Yılmaz, Mustafa Sefa Kılıç
Stevia extracts are obtained from Stevia rebaudiana commonly used as natural sweeteners. It is ∼250-300 times sweeter than sucrose. Common use of stevia prompted us to investigate its genotoxicity in human peripheral blood lymphocytes. Stevia (active ingredient steviol glycoside) was dissolved in pure water. Dose selection was done using ADI (acceptable daily intake) value. Negative control (pure water), 1, 2, 4, 8 and 16 μg/ml concentrations which were equivalent to ADI/4, ADI/2, ADI, ADI × 2 and ADI × 4 of Stevia were added to whole-blood culture...
July 24, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28737573/investigating-the-impact-of-different-suspicion-of-infection-criteria-on-the-accuracy-of-quick-sepsis-related-organ-failure-assessment-systemic-inflammatory-response-syndrome-and-early-warning-scores
#16
Matthew M Churpek, Ashley Snyder, Sarah Sokol, Natasha N Pettit, Dana P Edelson
OBJECTIVE: Studies in sepsis are limited by heterogeneity regarding what constitutes suspicion of infection. We sought to compare potential suspicion criteria using antibiotic and culture order combinations in terms of patient characteristics and outcomes. We further sought to determine the impact of differing criteria on the accuracy of sepsis screening tools and early warning scores. DESIGN: Observational cohort study. SETTING: Academic center from November 2008 to January 2016...
July 21, 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/28737003/facilitators-and-barriers-of-participation-in-a-longitudinal-research-on-migrant-families-in-badalona-spain-a-qualitative-approach
#17
Cristina Hernando, Meritxell Sabidó, Jordi Casabona
Migrant populations are under-represented in health research. We conducted a community-based qualitative study with the aim to examine the willingness, barriers, facilitators and reasons for participating in longitudinal health research among migrants. In Badalona and Santa Coloma de Gramanet (Barcelona) from May to November 2014, 26 individual interviews and 8 discussion groups were conducted with a convenience sample of 76 migrants born in Colombia, Peru, Ecuador, Bolivia, Morocco, Pakistan and China; and 9 key informants...
July 24, 2017: Health & Social Care in the Community
https://www.readbyqxmd.com/read/28736863/no-associations-established-between-single-nucleotide-polymorphisms-in-human-toll-like-receptor-2-and-toll-interacting-protein-and-staphylococcus-aureus-bloodstream-infections
#18
Tom Eirik Smeland, Fredrik Müller, Anita Blomfeldt, Knut Stavem, Hege Vangstein Aamot
Staphylococcus aureus bloodstream infections (SABSI) are associated with high morbidity and mortality. The Toll-like receptor 2 (TLR2) and Toll-interacting protein (TOLLIP) are important in recognition and regulation of human innate immunity response to S. aureus. Single nucleotide polymorphisms (SNPs) in the TLR2 and TOLLIP encoding genes have been associated with disease, including BSI. The aim of this study was to examine potential associations between a selection of SNPs in the genes encoding TLR2 and TOLLIP, and predisposition, severity, and outcome of SABSI...
July 24, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28736246/in-vitro-osteoclastogenesis-from-gaucher-patients-cells-correlates-with-bone-mineral-density-but-not-with-chitotriosidase
#19
C Bondar, J Mucci, A Crivaro, M Ormazabal, R Ceci, B Oliveri, D González, P Rozenfeld
Gaucher disease (GD) is caused by mutations on the gene encoding for the lysosomal enzyme glucocerebrosidase. Type I GD (GD1) patients present anemia, hepatosplenomegaly and bone alterations. In spite of treatment, bone alterations in GD patients persist, including poor bone mineral density (BMD). Mechanisms leading to bone damage are not completely understood, but previous reports suggest that osteoclasts are involved. Chitotriosidase (CHIT) is the most reliable biomarker used in the follow up of patients, although its correlation with bone status is unknown...
July 20, 2017: Bone
https://www.readbyqxmd.com/read/28736161/-immortalization-of-erythroid-progenitors-for-in-vitro-large-scale-red-cell-production
#20
A Caulier, L Guyonneau Harmand, L Garçon
Population ageing and increase in cancer incidence may lead to a decreased availability of red blood cell units. Thus, finding an alternative source of red blood cells is a highly relevant challenge. The possibility to reproduce in vitro the human erythropoiesis opens a new era, particularly since the improvement in the culture systems allows to produce erythrocytes from induced-Pluripotent Stem Cells (iPSCs), or CD34(+) Hematopoietic Stem Cells (HSCs). iPSCs have the advantage of in vitro self-renewal, but lead to poor amplification and maturation defects (high persistence of nucleated erythroid precursors)...
July 20, 2017: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
keyword
keyword
103463
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"