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Microbiology laboratory quality

Nouf Al Saleem, Khaled Al-Surimi
Frequent, preventable medical errors can have an adverse effect on patient safety and quality as well as leading to wasted resources. In the laboratory, errors can occur at any stage of sample processing; pre-analytical, analytical, and post analytical stages. However evidence shows most of the laboratory errors occur during the pre-analytical stage. The receipt and processing of specimens is one of the main steps in the pre-analytical stage. Errors in this stage could be due to mislabeling, incorrect test entry and entering the wrong location, among other reasons...
2016: BMJ Quality Improvement Reports
Benedetta Mazzanti, Serena Urbani, Simone Dal Pozzo, Paola Bufano, Lara Ballerini, Alessia Gelli, Irene Sodi, Irene Donnini, Massimo Di Gioia, Stefano Guidi, Julien Camisani, Riccardo Saccardi
BACKGROUND: Clinical grade processing of harvested bone marrow is required in various clinical situations, particularly in the management of ABO mismatching in allogeneic haematopoietic stem cell transplantation (HSCT) and in regenerative medicine. MATERIAL AND METHODS: We report a single-centre experience using a fully automated, clinical grade, closed system (Sepax, Biosafe, Switzerland). From 2003 to 2015, 125 procedures were performed in our laboratory, including buffy-coat production for HSCT (n=58), regenerative medicine in an orthopaedic setting (n=54) and density-gradient separation in a trial for treatment of critical limb ischaemia (n=13)...
September 27, 2016: Blood Transfusion, Trasfusione del Sangue
Małgorzata Baćmaga, Jadwiga Wyszkowska, Jan Kucharski
Fungicides are considered to be effective crop protection chemicals in modern agriculture. However, they can also exert toxic effects on non-target organisms, including soil-dwelling microbes. Therefore, the environmental fate of fungicides has to be closely monitored. The aim of this study was to evaluate the influence of the Falcon 460 EC fungicide on microbial diversity, enzyme activity and resistance, and plant growth. Samples of sandy loam with pHKCl 7.0 were collected for laboratory analyses on experimental days 30, 60 and 90...
September 8, 2016: Ecotoxicology
Misleidis Sardiñas, Grechen García, María Rosarys Martínez, Raúl Díaz, Lilian M Mederos
BACKGROUND: Baciloscopy is the primary tool for pulmonary tuberculosis diagnosis, being this technique the most used internationally in the search for infectious cases. Quality control is the process of the rechecking smears by a highly qualified observer. AIM: To evaluate and highlight the importance of quality control of smear microscopy in the Provincial Laboratories diagnosticians of Tuberculosis in Cuba. METHODS: This study was conducted at the National Reference Laboratory and Research in Tuberculosis, Leprosy and Mycobacteria in the Institute of Tropical Medicine "Pedro Kouri", Havana, Cuba, Were evaluated 2676 smears received from January 2013 to December 2014, from Provincial Centers of Hygiene, Epidemiology and Microbiology of Cuba, including the special municipality Isla de la Juventud...
June 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Carole D Mitnick, Richard A White, Chunling Lu, Carly A Rodriguez, Jaime Bayona, Mercedes C Becerra, Marcos Burgos, Rosella Centis, Theodore Cohen, Helen Cox, Lia D'Ambrosio, Manfred Danilovitz, Dennis Falzon, Irina Y Gelmanova, Maria T Gler, Jennifer A Grinsdale, Timothy H Holtz, Salmaan Keshavjee, Vaira Leimane, Dick Menzies, Giovanni Battista Migliori, Meredith B Milstein, Sergey P Mishustin, Marcello Pagano, Maria I Quelapio, Karen Shean, Sonya S Shin, Arielle W Tolman, Martha L van der Walt, Armand Van Deun, Piret Viiklepp
Debate persists about monitoring method (culture or smear) and interval (monthly or less frequently) during treatment for multidrug-resistant tuberculosis (MDR-TB). We analysed existing data and estimated the effect of monitoring strategies on timing of failure detection.We identified studies reporting microbiological response to MDR-TB treatment and solicited individual patient data from authors. Frailty survival models were used to estimate pooled relative risk of failure detection in the last 12 months of treatment; hazard of failure using monthly culture was the reference...
October 2016: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
Loredana Stabili, Margherita Licciano, Maria Flavia Gravina, Adriana Giangrande
We investigated and compared, by laboratory experiments, the filter-feeding activity on bacteria by the solitary ascidian Styela plicata and the colonial ascidian Polyandrocarpa zorritensis. Clearance rates and retention efficiencies were estimated by using, as only food source, the bacterial species Vibrio alginolyticus selected on account of its importance in aquaculture pathogenicity. The Cmax was 1.4±0.17Lh(-1)g(-1) DW for S. plicata and 1.745Lh(-1)g(-1) DW for P. zorritensis. The highest retention efficiency was 41% corresponding to a removed bacterial biomass of 16...
August 20, 2016: Science of the Total Environment
Sharon Kirkby, Christine Biggs
BACKGROUND: Infants admitted to the neonatal intensive care unit (NICU) are more susceptible to infections due to immature immune systems or invasive procedures that compromise protection from bacteria. These infants may stay in the NICU for extended periods of time, are exposed to many caregivers, and may be exposed to other infections. Cell phone use by both family and staff introduce unwanted bacteria into the NICU environment, thereby becoming a threat to this high-risk population...
August 19, 2016: Advances in Neonatal Care: Official Journal of the National Association of Neonatal Nurses
Anna Lenart-Boroń, Anna A Wolanin, Łukasz Jelonkiewicz, Mirosław Żelazny
This 3-year study was aimed to understand the factors and mechanisms that cause the temporal changes in the concentration of microbiological indicators of water quality and nutrient concentration in selected sites of the Białka river catchment (southern Poland) situated in direct vicinity of the largest ski station in the region. The analysis comprised 35 sampling campaigns conducted in five sites. Water temperature, pH, and electrical conductivity were measured during sampling, laboratory analyses included determination of the selected nutrients content (NH4, NO3, NO2, PO4); and the number of mesophilic and psychrophilic bacteria, coliforms, fecal coliforms, and Escherichia coli...
2016: Water, Air, and Soil Pollution
Amy S Gargis, Lisa Kalman, Ira M Lubin
Clinical microbiology and public health laboratories are beginning to utilize next-generation sequencing (NGS) for a range of applications. This technology has the potential to transform the field by providing approaches that will complement, or even replace, many conventional laboratory tests. While the benefits of NGS are significant, the complexities of these assays require an evolving set of standards to assure testing quality. Regulatory and accreditation requirements, professional guidelines, and best practices that help to assure the quality of NGS-based tests are emerging...
August 10, 2016: Journal of Clinical Microbiology
Rafael Medina González, Nieves Orta Mira, María Del Remedio Guna Serrano, José-Carlos Latorre Martínez, Enrique Ruiz de Gopegui, María Rosario Ovies, Marta Poveda, Concepción Gimeno Cardona
Human immunodeficiency virus type 1 (HIV-1), hepatitis B virus (HBV) and hepatitis C virus (HCV) viral load determinations are among the most relevant markers for the follow up of patients infected with these viruses. External quality control tools are crucial to ensure the accuracy of results obtained by microbiology laboratories. This article summarizes the results obtained from the 2014 SEIMC (Spanish Society of Infectious Diseases and Clinical Microbiology) External Quality Control Programme for HIV-1, HCV, and HBV viral loads...
July 2016: Enfermedades Infecciosas y Microbiología Clínica
Enrique Ruiz de Gopegui Bordes, M Del Remedio Guna Serrano, Nieves Orta Mira, Rafael Medina González, María Rosario Ovies, Marta Poveda, Concepción Gimeno Cardona
The External Quality Control Program of the Spanish Society of Infectious Diseases and Clinical Microbiology (SEIMC) include controls for bacteriology, serology, mycology, parasitology, mycobacteria, virology and molecular microbiology. This article presents the most relevant conclusions and lessons from the 2014 controls. As a whole, the results obtained in 2014 confirm the excellent skill and good technical standards found in previous editions. However, erroneous results can be obtained in any laboratory and in clinically relevant determinations...
July 2016: Enfermedades Infecciosas y Microbiología Clínica
M J T Crobach, T Planche, C Eckert, F Barbut, E M Terveer, O M Dekkers, M H Wilcox, E J Kuijper
In 2009 the first European Society of Clinical Microbiology and Infectious Diseases (ESCMID) guideline for diagnosing Clostridium difficile infection (CDI) was launched. Since then newer tests for diagnosing CDI have become available, especially nucleic acid amplification tests. The main objectives of this update of the guidance document are to summarize the currently available evidence concerning laboratory diagnosis of CDI and to formulate and revise recommendations to optimize CDI testing. This update is essential to improve the diagnosis of CDI and to improve uniformity in CDI diagnosis for surveillance purposes among Europe...
August 2016: Clinical Microbiology and Infection
Juan Carlos Rodríguez, Miguel Ángel Bratos, Esperanza Merino, Carmen Ezpeleta
The introduction of mass spectrometry through MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) in the diagnosis of bacteraemia and fungaemia has represented a revolution due to the rapidity and reliability of the results that it can offer to microbiology services and laboratories through analysis of the mass spectrum of the bacterial protein directly from positive blood culture bottles. These data are more useful if they are used in conjunction with other techniques able to identify the antibiotic resistance pattern of the microorganism...
June 2016: Enfermedades Infecciosas y Microbiología Clínica
Francine Pratlong, Yves Balard, Patrick Lami, Loïc Talignani, Christophe Ravel, Jacques Dereure, Michèle Lefebvre, Ghislaine Serres, Patrick Bastien, Jean-Pierre Dedet
We report the development of a laboratory collection of Leishmania that was initiated in 1975 and, after 39 years, has become an international Biological Resource Center (BRC-Leish, Montpellier, France, BioBank No. BB-0033-00052), which includes 6353 strains belonging to 36 Leishmania taxa. This is a retrospective analysis of the technical and organizational changes that have been adopted over time to take into account the technological advances and related modifications in the collection management and quality system...
July 5, 2016: Biopreservation and Biobanking
Alexandra S Simões, Isabel Couto, Cristina Toscano, Elsa Gonçalves, Pedro Póvoa, Miguel Viveiros, Luís V Lapão
In Europe, each year, more than four milion patients acquire a healthcare-associated infection (HAI) and almost 40 thousand die as a direct consequence of it. Regardless of many stategies to prevent and control HAIs, they remain an important cause of morbidity and mortality worldwide with a significant economic impact: a recent estimate places it at the ten billion dollars/year. The control of HAIs requires a prompt and efficient identification of the etiological agent and a rapid communication with the clinician...
2016: Frontiers in Microbiology
Jérémie F Cohen, Nathalie Bertille, Robert Cohen, Martin Chalumeau
BACKGROUND: Group A streptococcus (GAS) accounts for 20% to 40% of cases of pharyngitis in children; the remaining cases are caused by viruses. Compared with throat culture, rapid antigen detection tests (RADTs) offer diagnosis at the point of care (within five to 10 minutes). OBJECTIVES: To determine the diagnostic accuracy of RADTs for diagnosing GAS in children with pharyngitis. To assess the relative diagnostic accuracy of the two major types of RADTs (enzyme immunoassays (EIA) and optical immunoassays (OIA)) by indirect and direct comparison...
July 4, 2016: Cochrane Database of Systematic Reviews
Hideharu Shintani
Microbial testing performed in support of pharmaceutical and biopharmaceutical production falls into three main categories: detection (qualitative), enumeration (quantitative), and characterization/identification. Traditional microbiological methods are listed in the compendia and discussed by using the conventional growth-based techniques, which are labor intensive and time consuming. In general, such tests require several days of incubation for microbial contamination (bioburden) to be detected, and therefore management seldom is able to take proactive corrective measures...
2016: Biocontrol Science
Lucius C Imoh, Mubanga Mutale, Christopher T Parker, Rajiv T Erasmus, Annalise E Zemlin
INTRODUCTION: Timeliness of laboratory results is crucial to patient care and outcome. Monitoring turnaround times (TAT), especially for emergency tests, is important to measure the effectiveness and efficiency of laboratory services. Laboratory-based clinical audits reveal opportunities for improving quality. Our aim was to identify the most critical steps causing a high TAT for cerebrospinal fluid (CSF) chemistry analysis in our laboratory. MATERIALS AND METHODS: A 6-month retrospective audit was performed...
2016: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
Elzbieta Stefaniuk, Anna Baraniak, Monika Fortuna, Waleria Hryniewicz
This study was conducted to compare of the yeasts identification results obtained with two new systems using the MALDI-TOF MS technique with the ones obtained using the routine identification methods of Candida spp. in clinical microbiology laboratories. All 124 Candida spp. isolates were recovered from the routine examination of clinical specimens in microbiological laboratories and collected in the Centre of Quality Control in Microbiology in Warsaw (Poland). Our findings confirm the high agreement (98%) of fungal identification using the standard, biochemistry laboratory methods and mass spectrometry technique...
2016: Polish Journal of Microbiology
K C Iregbu, P I Nwajiobi-Princewill
BACKGROUND: Hepatitis B viral infection is an old medical problem with worldwide distribution. It is usually diagnosed using serologic methods. However, the decision as to which patient to treat or not remains challenging due to the poor sensitivity of serologic markers as prognostic or severity markers. Viral load (VL) determination using polymerase chain reaction techniques is a useful tool in decision-making. AIM: To determine the proportion of hepatitis B-positive patients who fall into different care groups based on the Society for Gastroenterology and Hepatology in Nigeria (SOGHIN) and National Institute for Health and Care Excellence guidelines, respectively, using result of hepatitis B virus (HBV) DNA determination...
March 2016: Annals of Medical and Health Sciences Research
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