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Microbiology laboratory quality

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https://www.readbyqxmd.com/read/28616481/evidence-of-exposure-to-rickettsia-felis-in-australian-patients
#1
Yen Thon Teoh, Sze Fui Hii, Stephen Graves, Robert Rees, John Stenos, Rebecca J Traub
Rickettsia felis is an emerging zoonosis, causing flea-borne spotted fever (FBSF). Serological diagnosis is typically confounded by cross-reactivity with typhus group rickettsiae and prior to the development of specific serological methods, cases of FBSF in Australia were misdiagnosed. Patient sera tested between August 2010 and December 2013 and known to be seropositive to R. typhi by immunofluorescence antibody testing (IFAT) were subsequently retested against R. felis using an R. felis-specific IFAT. Sera of 49 patients were of a sufficient quality to be included in re-analysis...
December 2016: One Health
https://www.readbyqxmd.com/read/28605496/validation-of-microbiological-testing-in-cardiovascular-tissue-banks-results-of-a-quality-round-trial
#2
Theo M M H de By, Carl McDonald, Susanne Süßner, Jill Davies, Wee Ling Heng, Ramadan Jashari, Ad J J C Bogers, Pieter Petit
OBJECTIVES: Surgeons needing human cardiovascular tissue for implantation in their patients are confronted with cardiovascular tissue banks that use different methods to identify and decontaminate micro-organisms. To elucidate these differences, we compared the quality of processing methods in 20 tissue banks and 1 reference laboratory. We did this to validate the results for accepting or rejecting tissue. We included the decontamination methods used and the influence of antibiotic cocktails and residues with results and controls...
June 12, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28592550/validation-and-implementation-of-clinical-laboratory-improvements-act-clia-compliant-whole-genome-sequencing-in-public-health-microbiology-laboratory
#3
Varvara K Kozyreva, Chau-Linda Truong, Alexander L Greninger, John Crandall, Rituparna Mukhopadhyay, Vishnu Chaturvedi
Public health microbiology laboratories (PHL) are on the cusp of unprecedented improvements in pathogen identification, antibiotic resistance detection, and outbreak investigation by using whole genome sequencing (WGS). However, considerable challenges remain due to the lack of common standards. Herein, we describe the validation of WGS on Illumina platform for routine use in PHL according to the Clinical Laboratory Improvements Act (CLIA) guidelines for the laboratory developed tests (LDTs). We developed a validation panel comprising ten Enterobacteriaceae isolates, five Gram-positive cocci, five Gram-negative non-fermenting species, nine Mycobacterium tuberculosis, and five miscellaneous bacteria...
June 7, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28592223/feline-leprosy-due-to-mycobacterium-lepraemurium
#4
Carolyn R O'Brien, Richard Malik, Maria Globan, George Reppas, Christina McCowan, Janet A Fyfe
OBJECTIVES: This paper, the second in a series of three on 'feline leprosy', provides a detailed description of disease referable to Mycobacterium lepraemurium, the most common cause of feline leprosy worldwide. METHODS: Cases were sourced retrospectively and prospectively for this observational study, describing clinical, geographical and molecular microbiological data for cats definitively diagnosed with M lepraemurium infection. RESULTS: A total of 145 cases of feline leprosy were scrutinised; 114 'new' cases were sourced from the Victorian Infectious Diseases Reference Laboratory records, veterinary pathology laboratories or veterinarians, and 31 cases were derived from six published studies...
July 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28591662/a-loop-mediated-isothermal-amplification-lamp-assay-for-the-rapid-detection-of-enterococcus-spp-in-water
#5
Roland Martzy, Claudia Kolm, Kurt Brunner, Robert L Mach, Rudolf Krska, Hana Šinkovec, Regina Sommer, Andreas H Farnleitner, Georg H Reischer
Faecal pollution of water and the resulting potential presence of human enteric pathogens is a predominant threat to public health. Microbiological water quality can be assessed by the detection of standard faecal indicator bacteria (SFIB) such as E. coli or certain Enterococcus species. In recent years, isothermal amplification methods have become a useful alternative to polymerase chain reaction (PCR), allowing molecular diagnostics with simple or no instrumentation. In this study, a novel screening method for the molecular detection of Enterococcus spp...
May 13, 2017: Water Research
https://www.readbyqxmd.com/read/28559163/a-cost-saving-strategy-for-processing-isolated-uropathogens-in-community-acquired-urinary-tract-infections
#6
Juan Manuel Sánchez-Calvo, José Luís de Francisco, Eva Torres-Martos, Juan Carlos Alados Arboledas, Maria Dolores López Prieto
Outpatient urine samples are among the most commonly processed in a microbiology laboratory, which involves a high economic burden. The aim of this study was compare cost and efficiency to process uropathogens between MicroScan system (2010-2011) versus a chromogenic medium and the disk diffusion method (2013-2014). In the first period, a total 9918 bacterial populations were isolated from urine samples. Annual estimated costs during 2010 and 2011 for processing were EUR 53,818 and EUR 57,306, respectively (EUR 111,124 total)...
May 27, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28539190/water-sanitation-and-hygiene-in-schools-status-and-implications-of-low-coverage-in-ethiopia-kenya-mozambique-rwanda-uganda-and-zambia
#7
Camille Morgan, Michael Bowling, Jamie Bartram, Georgia Lyn Kayser
Adequate access to water, sanitation, and hygiene (WaSH) in schools impacts health, educational outcomes, and gender disparities. Little multi-country research has been published on WaSH in rural schools in Sub-Saharan Africa. In this multi-national cross-sectional WaSH study, we document WaSH access, continuity, quality, quantity, and reliability in 2270 schools that were randomly sampled in rural regions of six Sub-Saharan African countries: Ethiopia, Kenya, Mozambique, Rwanda, Uganda, and Zambia. Data collection included: school WaSH surveys containing internationally established WaSH indicators, direct observation, and field- and laboratory-based microbiological water quality testing...
April 19, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28501335/obtaining-of-granular-fertilizers-based-on-ashes-from-combustion-of-waste-residues-and-ground-bones-using-phosphorous-solubilization-by-bacteria-bacillus-megaterium
#8
M Rolewicz, P Rusek, K Borowik
The article presents research results on obtaining phosphorus granulated fertilizers on the basis of microbiologically activated sewage sludge ashes, ground bones and dried blood from meat industry. Granulation tests were carried out using a laboratory pan granulator as well as on an experimental pilot plant. The aim of the studies was to select the proper composition of the mixture of raw materials and binding agents to obtain granulated fertilizers from waste materials such as MSSA and MBM and bacteria lyophilisate...
May 10, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28500818/household-microbial-water-quality-testing-in-a-peruvian-demographic-and-health-survey-evaluation-of-the-compartment-bag-test-for-escherichia-coli
#9
Alice Wang, Lanakila McMahan, Shea Rutstein, Christine Stauber, Jorge Reyes, Mark D Sobsey
AbstractThe Joint Monitoring Program relies on household surveys to classify access to improved water sources instead of measuring microbiological quality. The aim of this research was to pilot a novel test for Escherichia coli quantification of household drinking water in the 2011 Demographic and Health Survey (DHS) in Peru. In the Compartment Bag Test (CBT), a 100-mL water sample is supplemented with chromogenic medium to support the growth of E. coli, poured into a bag with compartments, and incubated. A color change indicates E...
April 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28465304/invasive-meningococcal-disease-in-the-veneto-region-of-italy-a-capture-recapture-analysis-for-assessing-the-effectiveness-of-an-integrated-surveillance-system
#10
Tatjana Baldovin, Roberta Lazzari, Silvia Cocchio, Patrizia Furlan, Chiara Bertoncello, Mario Saia, Francesca Russo, Vincenzo Baldo
BACKGROUND: Epidemiology of Neisseria meningitidis has been changing since the introduction of universal vaccination programmes against meningococcal serogroup C (MenC) and meningococcal serogroup B (MenB) has now become dominant. This study aimed to analyse the cases reported in institutional data recording systems to estimate the burden of invasive meningococcal diseases (IMDs) and assess the effectiveness of surveillance in Veneto region (Italy). METHODS: Analysis was performed from 2007 to 2014 on data recorded in different systems: Mandatory Notification System, National Surveillance of Invasive Bacterial Diseases System and Laboratories Surveillance System (LSS), which were pooled into a combined surveillance system (CSS) and hospital discharge records (HDRs)...
May 2, 2017: BMJ Open
https://www.readbyqxmd.com/read/28455579/-new-microbiological-techniques
#11
REVIEW
S Schubert, A Wieser, G Bonkat
Microbiological diagnostic procedures have changed rapidly in recent years. This is especially true in the field of molecular diagnostics. Classical culture-based techniques are still the gold standard in many areas; however, they are already complemented by automated and also molecular techniques to guarantee faster and better quality results. The most commonly used techniques include real-time polymerase chain reaction (RT-PCR) based systems and nucleic acid hybridization. These procedures are used most powerfully from direct patient samples or in assays to detect the presence of nonculturable or fastidious organisms...
April 28, 2017: Der Urologe. Ausg. A
https://www.readbyqxmd.com/read/28438086/feline-leprosy-due-to-candidatus-mycobacterium-tarwinense
#12
Carolyn R O'Brien, Richard Malik, Maria Globan, George Reppas, Christina McCowan, Janet A Fyfe
OBJECTIVES: This paper, the first in a series of three on 'feline leprosy', provides a detailed description of disease referable to Candidatus 'Mycobacterium tarwinense', the most common cause of feline leprosy in Victoria, Australia. METHODS: Cases were sourced retrospectively and prospectively for this observational study, describing clinical, geographical and molecular microbiological data for cats definitively diagnosed with Candidatus 'M tarwinense' infection...
May 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28423881/effects-of-calcium-propionate-on-the-fermentation-quality-and-aerobic-stability-of-alfalfa-silage
#13
Zhi Hao Dong, Xian Jun Yuan, Ai You Wen, Seare T Desta, Tao Shao
Objective: To assess the potency of calcium propionate used as silage additive, an experiment was carried out to evaluate the effect of calcium propionate on the nitrogen transformation, fermentation quality and aerobic stability of alfalfa silages. Methods: Alfalfa was ensiled with four levels of calcium propionate (CAP; 5, 10, 15 and 20 g kg-1 of fresh weigh) in laboratory silos for 30 days. After opening, the silages were analyzed for the chemical and microbiological characteristics, and subjected to an aerobic stability test...
March 25, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28412378/project-management-importance-for-diagnostic-laboratories
#14
REVIEW
Antony Croxatto, Gilbert Greub
BACKGROUND: The constraints applied on diagnostic laboratories to improve both quality and productivity together with personal shortages incite laboratory managers to constantly optimize the laboratory workflows, organization and technology. These continuous modifications of the laboratories should be conducted using efficient project and change management approaches in order to maximize the opportunities to achieve the project successfully. AIM: This review aims at presenting a general overview of project management with an emphasis on selected critical aspects...
April 12, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28404682/comparison-of-eplex-respiratory-pathogen-panel-with-laboratory-developed-real-time-pcr-assays-for-detection-of-respiratory-pathogens
#15
R H T Nijhuis, D Guerendiain, E C J Claas, K E Templeton
Infections of the respiratory tract can be caused by a diversity of pathogens, both viral and bacterial. Rapid microbiological diagnosis ensures appropriate antimicrobial therapy as well as effective implementation of isolation precautions. The ePlex respiratory pathogen panel (RP panel) is a novel molecular biology-based assay, developed by GenMark Diagnostics, Inc. (Carlsbad, CA), to be performed within a single cartridge for the diagnosis of 25 respiratory pathogens (viral and bacterial). The objective of this study was to compare the performance of the RP panel with those of laboratory-developed real-time PCR assays, using a variety of previously collected clinical respiratory specimens...
June 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28400009/autochthonous-lactic-acid-bacteria-with-probiotic-aptitudes-as-starter-cultures-for-fish-based-products
#16
Barbara Speranza, Angela Racioppo, Luciano Beneduce, Antonio Bevilacqua, Milena Sinigaglia, Maria Rosaria Corbo
This study focused on the selection of lactic starters with probiotic properties for the production of fermented fish-products by the use of a multivariate approach (Cluster Analysis and Principal Component Analysis). Seventy-five isolates were recovered from fish intestinal microbiota and characterized by evaluating phenotypical, technological and probiotic traits; the most promising isolates were molecularly identified and then used into fish fermented sausage production. Namely, data from technological characterization were modelled through Growth Index and used as input to run a preliminary selection...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28392724/an-overview-of-the-european-organization-for-external-quality-assurance-providers-in-laboratory-medicine-eqalm
#17
REVIEW
Anne Stavelin, Xavier Albe, Piet Meijer, Erika Sarkany, Finlay MacKenzie
The European Organisation for External Quality Assurance Providers in Laboratory Medicine (EQALM) was founded in 1996 and currently has members from 29 European countries and 6 countries from outside Europe. EQALM provides a forum for co-operation and exchange of knowledge on quality-related matters in laboratory medicine, especially with regard to external quality assessment (EQA) programs in Europe. In addition, EQALM represent the EQA providers in laboratory medicine at European level vis-ŕ-vis political, professional, scientific and other bodies, including patients' organisations...
February 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/28366154/-evaluation-of-mycobacterial-microscopy-and-culture-results-of-sureyyapasa-chest-diseases-and-chest-surgery-training-and-research-hospital-a-3-year-analysis
#18
Elçin Akduman Alaşehir, Ahmet Balıkçı, Mualla Partal, Gülay Çatmabacak, Görkem Yaman
Introduction: Effective diagnosis of tuberculosis is of great importance for transmission control and treatment success. The purpose of this study is to evaluate microscopic examination results of Ehrlich-Ziehl Neelsen (EZN) and Auramine-Rhodamine staining methods and automated BACTEC MGIT 960™ system and Löwenstein-Jensen (L-J) culture results of various clinical samples in the light of recent data from the world and Turkey. Materials and Methods: Specimens that were sent from various clinics to Sureyyapasa Chest Diseases and Chest Surgery Training and Research Hospital Microbiology Laboratory from January 2012 to December 2015 were evaluated retrospectively...
September 2016: Tüberküloz Ve Toraks
https://www.readbyqxmd.com/read/28259421/comparing-different-methods-for-fast-screening-of-microbiological-quality-of-beach-sand-aimed-at-rapid-response-remediation
#19
COMPARATIVE STUDY
Renan C Testolin, Tito C M Almeida, Marcus Polette, Joaquim O Branco, Larissa L Fischer, Guilherme Niero, Gabriel Poyer-Radetski, Valéria C Silva, Cleder A Somensi, Albertina X R Corrêa, Rogério Corrêa, Leonardo R Rörig, Ana Gabriela Itokazu, Jean-François Férard, Sylvie Cotelle, Claudemir M Radetski
There is scientific evidence that beach sands are a significant contributor to the pathogen load to which visitors are exposed. To develop beach quality guidelines all beach zones must be included in microbiological evaluations, but monitoring methods for beach sand quality are relatively longstanding, expensive, laborious and require moderate laboratory infrastructure. This paper aimed to evaluate the microorganism activity in different beach zones applying and comparing a classical method of membrane filtration (MF) with two colorimetric screening methods based on fluorescein (FDA) and tetrazolium (TTC) salt biotransformation to evaluate a new rapid and low-cost method for beach sand microbiological contamination assessments...
May 15, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28214393/flow-cytometric-bacterial-cell-counts-challenge-conventional-heterotrophic-plate-counts-for-routine-microbiological-drinking-water-monitoring
#20
REVIEW
S Van Nevel, S Koetzsch, C R Proctor, M D Besmer, E I Prest, J S Vrouwenvelder, A Knezev, N Boon, F Hammes
Drinking water utilities and researchers continue to rely on the century-old heterotrophic plate counts (HPC) method for routine assessment of general microbiological water quality. Bacterial cell counting with flow cytometry (FCM) is one of a number of alternative methods that challenge this status quo and provide an opportunity for improved water quality monitoring. After more than a decade of application in drinking water research, FCM methodology is optimised and established for routine application, supported by a considerable amount of data from multiple full-scale studies...
April 15, 2017: Water Research
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