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Microbiology laboratory quality

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https://www.readbyqxmd.com/read/28423881/effects-of-calcium-propionate-on-the-fermentation-quality-and-aerobic-stability-of-alfalfa-silage
#1
Zhi Hao Dong, Xian Jun Yuan, Ai You Wen, Seare T Desta, Tao Shao
Objective: To assess the potency of calcium propionate used as silage additive, an experiment was carried out to evaluate the effect of calcium propionate on the nitrogen transformation, fermentation quality and aerobic stability of alfalfa silages. Methods: Alfalfa was ensiled with four levels of calcium propionate (CAP; 5, 10, 15 and 20 g kg-1 of fresh weigh) in laboratory silos for 30 days. After opening, the silages were analyzed for the chemical and microbiological characteristics, and subjected to an aerobic stability test...
March 25, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28412378/project-management-importance-for-diagnostic-laboratories
#2
REVIEW
Antony Croxatto, Gilbert Greub
BACKGROUND: The constraints applied on diagnostic laboratories to improve both quality and productivity together with personal shortages incite laboratory managers to constantly optimize the laboratory workflows, organization and technology. These continuous modifications of the laboratories should be conducted using efficient project and change management approaches in order to maximize the opportunities to achieve the project successfully. AIM: This review aims at presenting a general overview of project management with an emphasis on selected critical aspects...
April 12, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28404682/comparison-of-the-eplex%C3%A2-respiratory-pathogen-panel-with-laboratory-developed-real-time-pcr-for-the-detection-of-respiratory-pathogens
#3
R H T Nijhuis, D Guerendiain, E C J Claas, K E Templeton
Infections of the respiratory tract can be caused by a diversity of pathogens, both viral and bacterial. Rapid microbiological diagnosis ensures appropriate antimicrobial therapy as well as effective implementation of isolation precautions. The ePlex® Respiratory Pathogen Panel (RP panel) is a novel molecular based assay, developed by GenMark Diagnostics Inc. (Carlsbad, California), to be performed within a single cartridge for the diagnosis of 25 respiratory pathogens (viral and bacterial). The objective of this study was to compare the performance of the RP panel with laboratory developed real-time PCR assays, using a variety of previously collected clinical respiratory specimens...
April 12, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28400009/autochthonous-lactic-acid-bacteria-with-probiotic-aptitudes-as-starter-cultures-for-fish-based-products
#4
Barbara Speranza, Angela Racioppo, Luciano Beneduce, Antonio Bevilacqua, Milena Sinigaglia, Maria Rosaria Corbo
This study focused on the selection of lactic starters with probiotic properties for the production of fermented fish-products by the use of a multivariate approach (Cluster Analysis and Principal Component Analysis). Seventy-five isolates were recovered from fish intestinal microbiota and characterized by evaluating phenotypical, technological and probiotic traits; the most promising isolates were molecularly identified and then used into fish fermented sausage production. Namely, data from technological characterization were modelled through Growth Index and used as input to run a preliminary selection...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28392724/an-overview-of-the-european-organization-for-external-quality-assurance-providers-in-laboratory-medicine-eqalm
#5
REVIEW
Anne Stavelin, Xavier Albe, Piet Meijer, Erika Sarkany, Finlay MacKenzie
The European Organisation for External Quality Assurance Providers in Laboratory Medicine (EQALM) was founded in 1996 and currently has members from 29 European countries and 6 countries from outside Europe. EQALM provides a forum for co-operation and exchange of knowledge on quality-related matters in laboratory medicine, especially with regard to external quality assessment (EQA) programs in Europe. In addition, EQALM represent the EQA providers in laboratory medicine at European level vis-ŕ-vis political, professional, scientific and other bodies, including patients' organisations...
February 15, 2017: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/28366154/-evaluation-of-mycobacterial-microscopy-and-culture-results-of-sureyyapasa-chest-diseases-and-chest-surgery-training-and-research-hospital-a-3-year-analysis
#6
Elçin Akduman Alaşehir, Ahmet Balıkçı, Mualla Partal, Gülay Çatmabacak, Görkem Yaman
Introduction: Effective diagnosis of tuberculosis is of great importance for transmission control and treatment success. The purpose of this study is to evaluate microscopic examination results of Ehrlich-Ziehl Neelsen (EZN) and Auramine-Rhodamine staining methods and automated BACTEC MGIT 960™ system and Löwenstein-Jensen (L-J) culture results of various clinical samples in the light of recent data from the world and Turkey. Materials and Methods: Specimens that were sent from various clinics to Sureyyapasa Chest Diseases and Chest Surgery Training and Research Hospital Microbiology Laboratory from January 2012 to December 2015 were evaluated retrospectively...
September 2016: Tüberküloz Ve Toraks
https://www.readbyqxmd.com/read/28259421/comparing-different-methods-for-fast-screening-of-microbiological-quality-of-beach-sand-aimed-at-rapid-response-remediation
#7
Renan C Testolin, Tito C M Almeida, Marcus Polette, Joaquim O Branco, Larissa L Fischer, Guilherme Niero, Gabriel Poyer-Radetski, Valéria C Silva, Cleder A Somensi, Albertina X R Corrêa, Rogério Corrêa, Leonardo R Rörig, Ana Gabriela Itokazu, Jean-François Férard, Sylvie Cotelle, Claudemir M Radetski
There is scientific evidence that beach sands are a significant contributor to the pathogen load to which visitors are exposed. To develop beach quality guidelines all beach zones must be included in microbiological evaluations, but monitoring methods for beach sand quality are relatively longstanding, expensive, laborious and require moderate laboratory infrastructure. This paper aimed to evaluate the microorganism activity in different beach zones applying and comparing a classical method of membrane filtration (MF) with two colorimetric screening methods based on fluorescein (FDA) and tetrazolium (TTC) salt biotransformation to evaluate a new rapid and low-cost method for beach sand microbiological contamination assessments...
March 2, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28214393/flow-cytometric-bacterial-cell-counts-challenge-conventional-heterotrophic-plate-counts-for-routine-microbiological-drinking-water-monitoring
#8
REVIEW
S Van Nevel, S Koetzsch, C R Proctor, M D Besmer, E I Prest, J S Vrouwenvelder, A Knezev, N Boon, F Hammes
Drinking water utilities and researchers continue to rely on the century-old heterotrophic plate counts (HPC) method for routine assessment of general microbiological water quality. Bacterial cell counting with flow cytometry (FCM) is one of a number of alternative methods that challenge this status quo and provide an opportunity for improved water quality monitoring. After more than a decade of application in drinking water research, FCM methodology is optimised and established for routine application, supported by a considerable amount of data from multiple full-scale studies...
April 15, 2017: Water Research
https://www.readbyqxmd.com/read/28177854/risk-factors-for-30-day-hospital-re-admission-with-an-infectious-complication-after-lower-extremity-vascular-procedures
#9
Joseph C Melvin, Jamie B Smith, Robin L Kruse, Todd R Vogel
BACKGROUND: Lowering the 30-d re-admission rate after vascular surgery offers the potential to improve healthcare quality. This study evaluated re-admission associated with infections after open and endovascular lower extremity (LE) procedures for peripheral artery disease (PAD). METHODS: Patients admitted for elective LE procedures for PAD were selected from the Cerner Health Facts(®) database. Chi-square analysis evaluated the characteristics of the index admission associated with infection at 30-d re-admission...
April 2017: Surgical Infections
https://www.readbyqxmd.com/read/28169558/validation-of-metagenomic-next-generation-sequencing-tests-for-universal-pathogen-detection
#10
Robert Schlaberg, Charles Y Chiu, Steve Miller, Gary W Procop, George Weinstock
CONTEXT: -Metagenomic sequencing can be used for detection of any pathogens using unbiased, shotgun next-generation sequencing (NGS), without the need for sequence-specific amplification. Proof-of-concept has been demonstrated in infectious disease outbreaks of unknown causes and in patients with suspected infections but negative results for conventional tests. Metagenomic NGS tests hold great promise to improve infectious disease diagnostics, especially in immunocompromised and critically ill patients...
February 7, 2017: Archives of Pathology & Laboratory Medicine
https://www.readbyqxmd.com/read/28161601/messages-from-the-first-international-conference-on-clinical-metagenomics-iccmg
#11
Etienne Ruppé, Gilbert Greub, Jacques Schrenzel
Metagenomics is recently entering in the clinical microbiology and an increasing number of diagnostic laboratories are now proposing the sequencing & annotation of bacterial genomes and/or the analysis of clinical samples by direct or PCR-based metagenomics with short time to results. In this context, the first International Conference on Clinical Metagenomics (ICCMg) was held in Geneva in October 2016 and several key aspects have been discussed including: i) the need for improved resolution, ii) the importance of interpretation given the common occurrence of sequence contaminants, iii) the need for improved bioinformatic pipelines, iv) the bottleneck of DNA extraction, v) the importance of gold standards, vi) the need to further reduce time to results, vii) how to improve data sharing, viii) the applications of bacterial genomics and clinical metagenomics in better adapting therapeutics and ix) the impact of metagenomics and new sequencing technologies in discovering new microbes...
February 1, 2017: Microbes and Infection
https://www.readbyqxmd.com/read/28149266/national-external-quality-assurance-program-pakistan-neqapp-a-milestone-in-proficiency-testing-in-pakistan
#12
Muhammad Usman Munir, Aamir Ijaz
OBJECTIVE: The objective of this study was to highlight current status and importance of National External Quality Assurance Program Pakistan (NEQAPP). Study Design: Cross sectional study. PLACE AND DURATION OF STUDY: Department of Chemical Pathology and Endocrinology, Armed Forces Institute of Pathology (AFIP) from August to October 2015. METHODS: The study data was extracted from electronic NEQAPP database. Results from 2014-2015 were evaluated for clinical chemistry, hematology, microbiology, and immunoassay programs...
December 2016: EJIFCC
https://www.readbyqxmd.com/read/28133869/diagnostic-microbiology-in-veterinary-dermatology-present-and-future
#13
REVIEW
Luca Guardabassi, Peter Damborg, Ivonne Stamm, Peter A Kopp, Els M Broens, Pierre-Louis Toutain
BACKGROUND: The microbiology laboratory can be perceived as a service provider rather than an integral part of the healthcare team. OBJECTIVES: The aim of this review is to discuss the current challenges of providing a state-of-the-art diagnostic veterinary microbiology service including the identification (ID) and antimicrobial susceptibility testing (AST) of key pathogens in veterinary dermatology. METHODS: The Study Group for Veterinary Microbiology (ESGVM) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) identified scientific, technological, educational and regulatory issues impacting the predictive value of AST and the quality of the service offered by microbiology laboratories...
February 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/28043600/benefits-and-challenges-of-molecular-diagnostics-for-childhood-tuberculosis
#14
Cristina Gutierrez
Expanding tuberculosis (TB)-diagnostic services, including access to rapid tests, is a World Health Organization (WHO) strategy to accelerate progress toward ending TB. Faster and more sensitive molecular tests capable of diagnosing TB and drug-resistant TB have the technical capacity to address limitations associated with smears and cultures by increasing accuracy and shortening turnaround times as compared with those of these conventional laboratory methods. Nucleic acid amplification assays used to detect and analyze Mycobacterium tuberculosis (MTB)-complex nucleic acids can be used directly on specimens from patients suspected of having TB...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28017464/a-review-of-pat-strategies-in-secondary-solid-oral-dosage-manufacturing-of-small-molecules
#15
REVIEW
Stephan Laske, Amrit Paudel, Otto Scheibelhofer
Pharmaceutical solid oral dosage product manufacturing is a well-established, yet revolutionizing area. To this end, process analytical technology (PAT) involves interdisciplinary and multivariate (chemical, physical, microbiological, and mathematical) methods for material (e.g., materials, intermediates, products) and process (e.g., temperature, pressure, throughput, etc.) analysis. This supports rational process modeling and enhanced control strategies for improved product quality and process efficiency. Therefore, it is often difficult to orient and find the relevant, integrated aspects of the current state-of-the-art...
November 22, 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28003040/nutritional-and-microbiological-quality-of-commercial-and-homemade-blenderized-whole-food-enteral-diets-for-home-based-enteral-nutritional-therapy-in-adults
#16
Maricy Machado Cavalca Vieira, Valdirene Francisca Neves Santos, Andrea Bottoni, Tania Beninga Morais
BACKGROUND & AIMS: Serious nutritional and contamination risks may be involved in the preparation of blenderized tube-feeding diets and in the handling of commercial diets. Their nutritional and microbiological quality in home settings is unknown. The objective of this study was to assess the nutritional and microbiological quality of commercial enteral and homemade blenderized whole foods diets intended to adult patients in home nutritional therapy. METHODS: In a cross sectional study, 66 samples of commercial (CD) and noncommercial (NCD) enteral diets were collected at the homes of patients in home enteral nutritional therapy, 33 of each type...
December 1, 2016: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/27932683/survival-of-bacteria-of-laboratory-animal-origin-on-cage-bedding-and-inactivation-by-hydrogen-peroxide-vapour
#17
Laurentiu Benga, W Peter M Benten, Eva Engelhardt, Christina Gougoula, Roland Schulze-Röbbecke, Martin Sager
This study aims to determine the ability of laboratory animal bacteria to resist desiccation and inactivation by hydrogen peroxide vapour (HPV) on paper bedding pieces. Bedding pieces were saturated with bacterial suspensions in water or 2% (w/v) bovine serum albumin (BSA) in water, and held in a mouse facility. Viable counts showed variable survival rates over time for the bacterial species used ([Pasteurella] pneumotropica, Muribacter muris, Pseudomonas aeruginosa, Acinetobacter redioresistens, Escherichia coli, Klebsiella oxytoca, Bordetella bronchiseptica, Bordetella hinzii, Enterococcus faecalis, β-haemolytic Streptococcus spp...
December 8, 2016: Laboratory Animals
https://www.readbyqxmd.com/read/27899431/monitoring-process-water-quality-using-near-infrared-spectroscopy-and-partial-least-squares-regression-with-prediction-uncertainty-estimation
#18
Peter B Skou, Thilo A Berg, Stina D Aunsbjerg, Dorrit Thaysen, Morten A Rasmussen, Frans van den Berg
Reuse of process water in dairy ingredient production-and food processing in general-opens the possibility for sustainable water regimes. Membrane filtration processes are an attractive source of process water recovery since the technology is already utilized in the dairy industry and its use is expected to grow considerably. At Arla Foods Ingredients (AFI), permeate from a reverse osmosis polisher filtration unit is sought to be reused as process water, replacing the intake of potable water. However, as for all dairy and food producers, the process water quality must be monitored continuously to ensure food safety...
March 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/27793578/diagnostic-value-of-sepsis-biomarkers-in-hematopoietic-stem-cell-transplant-recipients-in-a-condition-of-high-prevalence-of-gram-negative-pathogens
#19
Igor Stoma, Igor Karpov, Anatoly Uss, Oleg Rummo, Natalia Milanovich, Igor Iskrov
OBJECTIVE/BACKGROUND: A decision about the need for antimicrobial therapy in a patient with febrile neutropenia after hematopoietic stem cell transplantation (HSCT) is often complicated because of the low frequency of culture isolation and reduced clinical manifestation of infection. Usefulness and choice of sepsis biomarkers to distinguish bloodstream infection (BSI) from other causes of febrile episode is still argued in HSCT recipients in modern epidemiological situations characterized by the emergence of highly resistant gram-negative microorganisms...
March 2017: Hematology/oncology and Stem Cell Therapy
https://www.readbyqxmd.com/read/27777185/problems-associated-with-the-microbiological-control-of-the-breast-milk-from-hospital-milk-bank-units
#20
Didier Lecointe, Jade Assoukpa, Lauriane Cezard, Carole Descaves, Armelle Gouot, Sandrine Bourgeois, Monique Bergeon, Stéphane Koutcherenko, Yasmine Lakhdari, Noura Kassidi
The microbiological tests on breast milk performed when samples of pasteurized breast milk are added to hospital milk banks are covered by French regulations dating from December 3(rd) 2007. They involve counts of the aerobic total flora and of Staphylococcus aureus in a sample of milk before pasteurization, and culture after pasteurization to check that the treated milk is sterile. The regulations specify the nature of the agar plates to be used, together with the conditions for plating and incubation, but they lack detail in other areas...
December 1, 2016: Annales de Biologie Clinique
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