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Microbiology laboratory quality

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https://www.readbyqxmd.com/read/29031605/a-waterborne-outbreak-of-multiple-diarrhoeagenic-escherichia-coli-infections-associated-with-drinking-water-at-a-school-camp
#1
Jungsun Park, Jin Seok Kim, Soojin Kim, Eunkyung Shin, Kyung-Hwan Oh, Yonghoon Kim, Cheon Hyeon Kim, Min Ah Hwang, Chan Mun Jin, Kyoungin Na, Jin Lee, Enhi Cho, Byung-Hak Kang, Hyo-Sun Kwak, Won Keun Seong, Junyoung Kim
BACKGROUND: In June 2015, a local public health laboratory was notified that students had developed gastroenteritis symptoms after participating in a camp. An outbreak investigation was conducted to determine the extent and cause of the outbreak. METHOD: We conducted a retrospective cohort study to determine the correlations between the illness and specific exposures at the school camp. All attendees were interviewed with a standard questionnaire that addressed clinical symptoms, food consumption and environmental exposures...
October 11, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29030529/microbiological-test-data-assuring-data-integrity
#2
Edward Charles Tidswell, Tim Sandle
Marketed drugs and devices possess specifications including critical microbiological quality attributes purposed to assure efficacy and patient safety. These attributes are legislated requirements intended to protect the recipient patient. Sampling, microbiological testing, interpretation of data for final products, raw materials and intermediates all contribute to a cohesive assessment in the assurance of finished product quality. Traditional culture-based microbiological methods possess inherent and unavoidable variability, recognized by the compendia and which might lead to erroneous conclusion pertaining to product quality...
October 12, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/29026355/colistin-and-polymyxin-b-minimal-inhibitory-concentrations-determined-by-etest-found-unreliable-for-gram-negative-bacilli
#3
Shelby Simar, Diane Sibley, Deborah Ashcraft, George Pankey
BACKGROUND: A reliable method of polymyxin B and E (colistin) susceptibility testing remains elusive. These drugs diffuse poorly into agar, creating potentially inaccurate Etest and disk diffusion results, and testing by these methods is not recommended. Broth microdilution is the reference testing method, although it can be sometimes difficult to interpret. Currently, when a colistin susceptibility test is ordered for a patient in the Ochsner Health System, our diagnostic microbiology laboratory performs the Etest...
2017: Ochsner Journal
https://www.readbyqxmd.com/read/29021895/readiness-for-antimicrobial-resistance-amr-surveillance-in-pakistan-a-model-for-laboratory-strengthening
#4
Dania Khalid Saeed, Rumina Hasan, Mahwish Naim, Afia Zafar, Erum Khan, Kausar Jabeen, Seema Irfan, Imran Ahmed, Mohammad Zeeshan, Zabin Wajidali, Joveria Farooqi, Sadia Shakoor, Abdul Chagla, Jason Rao
BACKGROUND: Limited capacity of laboratories for antimicrobial susceptibility testing (AST) presents a critical diagnostic bottleneck in resource limited countries. This paper aims to identify such gaps and to explore whether laboratory networks could contribute towards improving AST in low resource settings. METHODS: A self-assessment tool to assess antimicrobial susceptibility testing capacity was administered as a pre-workshop activity to participants from 30 microbiology laboratories in 3 cities in Pakistan...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29021151/rapid-nanopore-sequencing-of-plasmids-and-resistance-gene-detection-in-clinical-isolates
#5
Jamie K Lemon, Pavel P Khil, Karen M Frank, John P Dekker
Recent advances in nanopore sequencing technology have led to a substantial increase in throughput and sequence quality. Together, these improvements may permit real-time benchtop genomic sequencing and antimicrobial resistance gene detection in clinical isolates. Here, we evaluated workflows and turnaround times for a benchtop long-read sequencing approach in the clinical microbiology laboratory using the Oxford Nanopore Technologies MinION sequencer. We performed genomic and plasmid sequencing of three clinical isolates with both MinION and Illumina MiSeq, using different library preparation methods (2D and rapid 1D) with the goal of antimicrobial resistance gene detection...
October 11, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28954899/optimizing-test-utilization-in-the-clinical-microbiology-laboratory-tools-and-opportunities
#6
Bobbi S Pritt
Optimal laboratory test utilization is important for providing high quality clinical care and efficiently using limited health care resources. While microbiologists have long been advocates for appropriate laboratory test utilization, the wide-spread availability of electronic medical records capable of supporting clinician order entry and clinical decision support tools (CDSTs) have provided expanded opportunities for implementing effective, automated test stewardship protocols. In this issue of the Journal of Clinical Microbiology, D...
September 27, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28929962/-determination-of-in-vitro-susceptibilities-of-brucella-spp-strains-against-11-different-antibacterial-gents-isolated-from-blood-cultures
#7
Recep Keşli, Hüseyin Bilgin, Halim Yılmaz
Brucellosis is a worldwide zoonotic disease and still continuous to be a major public health problem. In this study, it was aimed to identify the Brucella strains to the species level isolated from blood cultures, and to determine the rate of antimicrobial susceptibility against eleven antibacterial agents. A total of 106 Brucella spp. strains were included in the study, which were isolated from blood cultures in University of Health Sciences, Konya Training and Research Hospital, Medical Microbiology Laboratory between January 2011 and June 2013...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28929961/-national-antimicrobial-resistance-surveillance-system-namrss-external-quality-assessment-studies-2011-2016
#8
Serap Süzük Yıldız, Hüsniye Şimşek, Nilay Çöplü, Zeynep Gülay
Establishment of sustainable and evidence-based surveillance systems are recommended for prevention of microbial resistance by the World Health Organization (WHO). As a necessity of these surveillance systems, participants are recommended to implement an external quality assessment (EQA) program. In this scope, National Antimicrobial Resistance Surveillance System (NARSS) has been established within the Public Health Institute of Turkey (PHIT) in our country since 2011. In the scope of this surveillance, NARSS EQA program has been implemented in a cycle per year and four isolates were sent to participants per cycle every year since 2011...
July 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28866114/traditional-approaches-versus-mass-spectrometry-in-bacterial-identification-and-typing
#9
REVIEW
Angela Sloan, Gehua Wang, Keding Cheng
Biochemical methods such as metabolite testing and serotyping are traditionally used in clinical microbiology laboratories to identify and categorize microorganisms. Due to the large variety of bacteria, identifying representative metabolites is tedious, while raising high-quality antisera or antibodies unique to specific biomarkers used in serotyping is very challenging, sometimes even impossible. Although serotyping is a certified approach for differentiating bacteria such as E. coli and Salmonella at the subspecies level, the method is tedious, laborious, and not practical during an infectious disease outbreak...
October 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/28840984/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-in-clinical-microbiology-what-are-the-current-issues
#10
REVIEW
Alex van Belkum, Martin Welker, David Pincus, Jean Philippe Charrier, Victoria Girard
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized the identification of microbial species in clinical microbiology laboratories. MALDI-TOF-MS has swiftly become the new gold-standard method owing to its key advantages of simplicity and robustness. However, as with all new methods, adoption of the MALDI-TOF MS approach is still not widespread. Optimal sample preparation has not yet been achieved for several applications, and there are continuing discussions on the need for improved database quality and the inclusion of additional microbial species...
November 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28820902/from-hospitalization-records-to-surveillance-the-use-of-local-patient-profiles-to-characterize-cholera-in-vellore-india
#11
Melissa S Cruz, Tania M AlarconFalconi, Meghan A Hartwick, Aishwarya Venkat, Hanna Y Ehrlich, Shalini Anandan, Honorine D Ward, Balaji Veeraraghavan, Elena N Naumova
Despite availability of high quality medical records, health care systems often do not have the resources or tools to utilize these data efficiently. Yet, hospital-based, laboratory-confirmed records may pave the way for building reliable surveillance systems capable of monitoring temporal trends of emerging infections. In this communication, we present a new tool to compress and visualize medical records with a local population profile (LPP) approach, which transforms information into statistically comparable patterns...
2017: PloS One
https://www.readbyqxmd.com/read/28801105/rectal-swab-screening-assays-of-public-health-importance-in-molecular-diagnostics-sample-adequacy-control
#12
Sanja Glisovic, Shaun Eintracht, Yves Longtin, Matthew Oughton, Ivan Brukner
Rectal swabs are routinely used by public health authorities to screen for multi-drug resistant enteric bacteria including vancomycin-resistant enterococci (VRE) and carbapenem-resistant enterobacteriaceae (CRE). Screening sensitivity can be influenced by the quality of the swabbing, whether performed by the patient (self-swabbing) or a healthcare practitioner. One common exclusion criterion for rectal swabs is absence of "visible soiling" from fecal matter. In our institution, this criterion excludes almost 10% of rectal swabs received in the microbiology laboratory...
August 8, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28761562/assessment-of-physicochemical-and-microbiological-quality-of-public-swimming-pools-in-addis-ababa-ethiopia
#13
Kokebe Yedeme, Melese Hailu Legese, Almaz Gonfa, Somson Girma
BACKGROUND: From swimming pools, bathers may acquire many potential pathogens or may be affected by the physicochemical characteristics of water used during bathing. Hence, this study aimed at assessing the physicochemical and microbiological quality of public swimming pools located at different hotels and recreation center in Addis Ababa, Ethiopia. METHOD: A cross sectional study was carried out from February to May, 2016. Nine hotels and one recreation center which recognized to have public swimming services were included...
2017: Open Microbiology Journal
https://www.readbyqxmd.com/read/28708567/%C3%AE-defensin-point-of-care-test-for-diagnosis-of-prosthetic-joint-infections-neglected-role-of-laboratory-and-clinical-pathologists
#14
REVIEW
Lorenzo Drago, Marco Toscano, Lorenza Tacchini, Giuseppe Banfi
Periprosthetic joint infection (PJI) is a serious complication that may occur after native joint replacement leading to a severe health and economic burden. Currently, due to several confounding factors, PJI is difficult to diagnose. Today, a multidisciplinary approach is indispensable to correctly define a periprosthetic joint infection; indeed, tissue histology, microbiology cultures and clinical findings are used together to achieve this goal. Analysis of α-defensin is commonly used for PJI diagnosis, as it allows the rapid detection of α-defensin present in the synovial fluid following a microbial infection...
July 14, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28666419/effectiveness-of-atp-bioluminescence-assay-for-presumptive-identification-of-microorganisms-in-hospital-water-sources
#15
Máira Gazzola Arroyo, Adriano Menis Ferreira, Oleci Pereira Frota, Marcelo Alessandro Rigotti, Denise de Andrade, Natalia Seron Brizzotti, Jacqueline Tanury Macruz Peresi, Elza Maria Castilho, Margarete Teresa Gottardo de Almeida
BACKGROUND: Laboratory analysis of organisms in water include arduous methods, such as the multiple tube and membrane filter. The ATP bioluminescence system, proposes a new way of measuring cellular material in water by measuring adenosine triphosphate (ATP) levels, which are expressed in relative light units (RLU). The ATP bioluminescence assay has been increasingly used to assess the microbiological safety of the hospital environment. However, there are few studies investigating the use of this methodology to evaluate the microbiological quality of water...
June 30, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28658391/escherichia-coli-is-not-a-suitable-fecal-indicator-to-assess-water-fecal-contamination-by-otters
#16
M Oliveira, D Freire, N M Pedroso
The detection of pathogenic microorganisms in aquatic environments is extremely relevant in terms of public health. As these laboratorial methodologies are usually difficult, expensive and time-consuming, they are frequently replaced by the assessment of fecal indicator bacteria, such as Escherichia coli. This study aimed to assess the presence of E. coli in fecal samples from Neotropical otters, to evaluate its potential as fecal indicator to be applied to the determination of water microbiological quality in areas where otters' populations are high...
June 26, 2017: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/28647228/chloramine-demand-estimation-using-surrogate-chemical-and-microbiological-parameters
#17
Sina Moradi, Sanly Liu, Christopher W K Chow, John van Leeuwen, David Cook, Mary Drikas, Rose Amal
A model is developed to enable estimation of chloramine demand in full scale drinking water supplies based on chemical and microbiological factors that affect chloramine decay rate via nonlinear regression analysis method. The model is based on organic character (specific ultraviolet absorbance (SUVA)) of the water samples and a laboratory measure of the microbiological (Fm) decay of chloramine. The applicability of the model for estimation of chloramine residual (and hence chloramine demand) was tested on several waters from different water treatment plants in Australia through statistical test analysis between the experimental and predicted data...
July 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28616481/evidence-of-exposure-to-rickettsia-felis-in-australian-patients
#18
Yen Thon Teoh, Sze Fui Hii, Stephen Graves, Robert Rees, John Stenos, Rebecca J Traub
Rickettsia felis is an emerging zoonosis, causing flea-borne spotted fever (FBSF). Serological diagnosis is typically confounded by cross-reactivity with typhus group rickettsiae and prior to the development of specific serological methods, cases of FBSF in Australia were misdiagnosed. Patient sera tested between August 2010 and December 2013 and known to be seropositive to R. typhi by immunofluorescence antibody testing (IFAT) were subsequently retested against R. felis using an R. felis-specific IFAT. Sera of 49 patients were of a sufficient quality to be included in re-analysis...
December 2016: One Health
https://www.readbyqxmd.com/read/28605496/validation-of-microbiological-testing-in-cardiovascular-tissue-banks-results-of-a-quality-round-trial
#19
Theo M M H de By, Carl McDonald, Susanne Süßner, Jill Davies, Wee Ling Heng, Ramadan Jashari, Ad J J C Bogers, Pieter Petit
OBJECTIVES: Surgeons needing human cardiovascular tissue for implantation in their patients are confronted with cardiovascular tissue banks that use different methods to identify and decontaminate micro-organisms. To elucidate these differences, we compared the quality of processing methods in 20 tissue banks and 1 reference laboratory. We did this to validate the results for accepting or rejecting tissue. We included the decontamination methods used and the influence of antibiotic cocktails and residues with results and controls...
June 12, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/28592550/validation-and-implementation-of-clinical-laboratory-improvements-act-compliant-whole-genome-sequencing-in-the-public-health-microbiology-laboratory
#20
Varvara K Kozyreva, Chau-Linda Truong, Alexander L Greninger, John Crandall, Rituparna Mukhopadhyay, Vishnu Chaturvedi
Public health microbiology laboratories (PHLs) are on the cusp of unprecedented improvements in pathogen identification, antibiotic resistance detection, and outbreak investigation by using whole-genome sequencing (WGS). However, considerable challenges remain due to the lack of common standards. Here, we describe the validation of WGS on the Illumina platform for routine use in PHLs according to Clinical Laboratory Improvements Act (CLIA) guidelines for laboratory-developed tests (LDTs). We developed a validation panel comprising 10 Enterobacteriaceae isolates, 5 Gram-positive cocci, 5 Gram-negative nonfermenting species, 9 Mycobacterium tuberculosis isolates, and 5 miscellaneous bacteria...
August 2017: Journal of Clinical Microbiology
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