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Microbiology laboratory quality

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https://www.readbyqxmd.com/read/29620501/are-commercial-providers-a-viable-option-for-clinical-bacterial-sequencing
#1
Kathy Raven, Beth Blane, Carol Churcher, Julian Parkhill, Sharon J Peacock
Bacterial whole-genome sequencing in the clinical setting has the potential to bring major improvements to infection control and clinical practice. Sequencing instruments are not currently available in the majority of routine microbiology laboratories worldwide, but an alternative is to use external sequencing providers. To foster discussion around this we investigated whether send-out services were a viable option. Four providers offering MiSeq sequencing were selected based on cost and evaluated based on the service provided and sequence data quality...
April 5, 2018: Microbial Genomics
https://www.readbyqxmd.com/read/29535998/networking-of-public-health-microbiology-laboratories-bolsters-europe-s-defenses-against-infectious-diseases
#2
Barbara Albiger, Joana Revez, Katrin Claire Leitmeyer, Marc J Struelens
In an era of global health threats caused by epidemics of infectious diseases and rising multidrug resistance, microbiology laboratories provide essential scientific evidence for risk assessment, prevention, and control. Microbiology has been at the core of European infectious disease surveillance networks for decades. Since 2010, these networks have been coordinated by the European Centre for Disease Prevention and Control (ECDC). Activities delivered in these networks include harmonization of laboratory diagnostic, antimicrobial susceptibility and molecular typing methods, multicentre method validation, technical capacity mapping, training of laboratory staff, and continuing quality assessment of laboratory testing...
2018: Frontiers in Public Health
https://www.readbyqxmd.com/read/29519767/clinical-bacteriology-in-low-resource-settings-today-s-solutions
#3
REVIEW
Sien Ombelet, Jean-Baptiste Ronat, Timothy Walsh, Cedric P Yansouni, Janneke Cox, Erika Vlieghe, Delphine Martiny, Makeda Semret, Olivier Vandenberg, Jan Jacobs
Low-resource settings are disproportionately burdened by infectious diseases and antimicrobial resistance. Good quality clinical bacteriology through a well functioning reference laboratory network is necessary for effective resistance control, but low-resource settings face infrastructural, technical, and behavioural challenges in the implementation of clinical bacteriology. In this Personal View, we explore what constitutes successful implementation of clinical bacteriology in low-resource settings and describe a framework for implementation that is suitable for general referral hospitals in low-income and middle-income countries with a moderate infrastructure...
March 5, 2018: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/29514436/effect-of-cumin-essential-oil-usage-on-fermentation-quality-aerobic-stability-and-in-vitro-digetibility-of-alfalfa-silage
#4
Aslı Turan, Sibel Soycan Önenç
Objective: This study was carried out to determine the effects of cumin essential oil on the silage fermentation, aerobic stability and in vitro digestibility of alfalfa silages. Methods: Alfalfa was harvested at early bloom (5 th cutting) stage in October and wilted for about 3 hours. The research was carried out at three groups which were the control group where no additive control was done (CON), cumin essential oil (CMN3) with 300 mg/kg and CMN5 with 500 mg/kg cumin essential oil addition...
March 2, 2018: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/29493640/-the-interplay-of-diagnostic-and-antimicrobial-stewardship-for-the-management-of-septic-patients-the-tuscan-model
#5
Silvia Forni, Giulio Toccafondi, Bruno Viaggi, Maddalena Grazzini, Sara D'Arienzo, Fabrizio Gemmi, Andrea Vannucci, Giorgio Tulli
Antimicrobial resistance is a global threat caused by the rapid spread of multiresistant microorganisms. Antimicrobial stewardship (AS) is a coordinated intervention designed to improve the appropriate use of antimicrobials by promoting the selection of the optimal drug regimen, dose, duration of therapy and route of administration. AS programs have proved effective in reducing antimicrobial resistance, inappropriate antimicrobial use and in improving patient outcomes. Recently developed rapid diagnostic technologies in microbiology (RDTM) allows a faster and etiological diagnosis of infection and a reduction in the use of unnecessary empirical therapies...
February 2018: Recenti Progressi in Medicina
https://www.readbyqxmd.com/read/29457001/control-of-infectious-diseases-in-the-era-of-european-clinical-microbiology-laboratory-consolidation-new-challenges-and-opportunities-for-the-patient-and-for-public-health-surveillance
#6
Olivier Vandenberg, Zisis Kozlakidis, Jacques Schrenzel, Marc Jean Struelens, Judith Breuer
Many new innovative diagnostic approaches have been made available during the last 10 years with major impact on patient care and public health surveillance. In parallel, to enhance the cost-effectiveness of the clinical microbiology laboratories (CMLs), European laboratory professionals have streamlined their organization leading to amalgamation of activities and restructuring of their professional relationships with clinicians and public health specialists. Through this consolidation process, an operational model has emerged that combines large centralized clinical laboratories performing most tests on one high-throughput analytical platform connected to several distal laboratories dealing locally with urgent analyses at near point of care...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29439746/antimicrobial-stewardship-program-implementation-of-a-quality-improvement-intervention-using-real-time-feedback-and-an-electronic-order-set-for-the-management-of-staphylococcus-aureus-bacteremia
#7
Rossana Rosa, Bruno Zavala, Natalie Cain, Shweta Anjan, Laura Aragon, Lilian M Abbo
Antimicrobial stewardship programs can optimize the management of Staphylococcus aureus bacteremia by integrating information technology and microbiology laboratory resources. This study describes our experience implementing an intervention consisting of real-time feedback and the use of an electronic order set for the management of S. aureus bacteremia. Infect Control Hosp Epidemiol 2018;39:346-349.
March 2018: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/29433835/towards-automated-detection-semi-quantification-and-identification-of-microbial-growth-in-clinical-bacteriology-a-proof-of-concept
#8
Antony Croxatto, Raphaël Marcelpoil, Cédrick Orny, Didier Morel, Guy Prod'hom, Gilbert Greub
BACKGROUND: Automation in microbiology laboratories impacts management, workflow, productivity and quality. Further improvements will be driven by the development of intelligent image analysis allowing automated detection of microbial growth, release of sterile samples, identification and quantification of bacterial colonies and reading of AST disk diffusion assays. We investigated the potential benefit of intelligent imaging analysis by developing algorithms allowing automated detection, semi-quantification and identification of bacterial colonies...
December 2017: Biomedical Journal
https://www.readbyqxmd.com/read/29433218/application-of-high-throughput-pyrosequencing-in-the-analysis-of-microbiota-of-food-commodities-procured-from-small-and-large-retail-outlets-in-a-u-s-metropolitan-area-a-pilot-study
#9
Daleniece Higgins, Chandan Pal, Irshad M Sulaiman, Chunrong Jia, Tyler Zerwekh, Scot E Dowd, Pratik Banerjee
With the advent of high-throughput sequencing technologies, it is possible to comprehensively analyze the microbial community of foods without culturing them in the laboratory. The estimation of all microbes inhabiting a food commodity (food microbiota) therefore may shed light on the microbial quality and safety of foods. In this study, we utilized high-throughput pyrosequencing of 16S rRNA genes as well as traditional microbiological methods to evaluate the bacterial diversity and the predicted metabolic pathways associated with the bacterial communities of selected foods (romaine lettuce, cabbage, deli meat, and chicken legs, total 200 samples) procured from small and large retail outlets located in Memphis-Shelby County, Tennessee, USA...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29428064/automatic-hemolysis-identification-on-aligned-dual-lighting-images-of-cultured-blood-agar-plates
#10
Mattia Savardi, Alessandro Ferrari, Alberto Signoroni
BACKGROUND AND OBJECTIVE: The recent introduction of Full Laboratory Automation systems in clinical microbiology opens to the availability of streams of high definition images representing bacteria culturing plates. This creates new opportunities to support diagnostic decisions through image analysis and interpretation solutions, with an expected high impact on the efficiency of the laboratory workflow and related quality implications. Starting from images acquired under different illumination settings (top-light and back-light), the objective of this work is to design and evaluate a method for the detection and classification of diagnostically relevant hemolysis effects associated with specific bacteria growing on blood agar plates...
March 2018: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/29426791/collection-transport-and-general-processing-of-clinical-specimens-in-microbiology-laboratory
#11
M Isabel Sánchez-Romero, Juan Manuel García-Lechuz Moya, Juan José González López, Nieves Orta Mira
The interpretation and the accuracy of the microbiological results still depend to a great extent on the quality of the samples and their processing within the Microbiology laboratory. The type of specimen, the appropriate time to obtain the sample, the way of sampling, the storage and transport are critical points in the diagnostic process. The availability of new laboratory techniques for unusual pathogens, makes necessary the review and update of all the steps involved in the processing of the samples. Nowadays, the laboratory automation and the availability of rapid techniques allow the precision and turn-around time necessary to help the clinicians in the decision making...
February 6, 2018: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29424725/sodium-hypochlorite-dosage-for-household-and-emergency-water-treatment-updated-recommendations
#12
Natalie Wilhelm, Anya Kaufmann, Elizabeth Blanton, Daniele Lantagne
Household water treatment with chlorine can improve the microbiological quality of household water and reduce diarrheal disease. We conducted laboratory and field studies to inform chlorine dosage recommendations. In the laboratory, reactors of varying turbidity (10-300 NTU) and total organic carbon (0-25 mg/L addition) were created, spiked with Escherichia coli, and dosed with 3.75 mg/L sodium hypochlorite. All reactors had >4 log reduction of E. coli 24 hours after chlorine addition. In the field, we tested 158 sources in 22 countries for chlorine demand...
February 2018: Journal of Water and Health
https://www.readbyqxmd.com/read/29424720/the-effect-of-sodium-thiosulfate-dechlorination-on-fecal-indicator-bacteria-enumeration-laboratory-and-field-data
#13
Anna L Murray, Emily Kumpel, Rachel Peletz, Ranjiv S Khush, Daniele S Lantagne
In microbiological water quality testing, sample dechlorination with sodium thiosulfate is recommended to ensure that results accurately reflect the water quality at sample collection. Nevertheless, monitoring institutions in low-resource settings do not always dechlorinate samples, and there is limited research describing how this practice impacts drinking water quality results. The effect of dechlorination on indicator bacteria counts was evaluated by spiking laboratory water with five Escherichia coli (E...
February 2018: Journal of Water and Health
https://www.readbyqxmd.com/read/29419793/microbiological-values-of-rainwater-harvested-in-adelaide
#14
Chirhakarhula Emmanuel Chubaka, Harriet Whiley, John W Edwards, Kirstin E Ross
In Australia, rainwater is an important source of water for many households. Unlike municipal water, rainwater is often consumed untreated. This study investigated the potential contamination of rainwater by microorganisms. Samples from 53 rainwater tanks across the Adelaide region were collected and tested using Colilert™ IDEXX Quanti-Tray*/2000. Twenty-eight out of the 53 tanks (53%) contained Escherichia coli. Samples collected from ten tanks contained E. coli at concentrations exceeding the limit of 150 MPN/100 mL for recreational water quality...
February 8, 2018: Pathogens
https://www.readbyqxmd.com/read/29410246/how-to-accreditation-of-blood-cultures-proceedings-a-clinical-microbiology-approach-for-adding-value-to-patient-care
#15
REVIEW
B Lamy, A Ferroni, C Henning, C Cattoen, P Laudat
BACKGROUND: Quality assurance and quality management are driving forces for controlling blood culture best practices but should not be disconnected from the end-point target, i.e. patient value. AIMS: This article is intended to help microbiologists implement blood culture accreditation that is actually beneficial to patient management. SOURCES: Experience from a nationwide taskforce for promoting quality assurance and competence in clinical microbiology laboratories, guidelines on blood culture...
February 2, 2018: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29391695/isolation-and-identification-of-a-cellulolytic-enterobacter-from-rumen-of-aceh-cattle
#16
Wenny Novita Sari, Safika, Darmawi, Yudha Fahrimal
Aims: The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen fluid of Aceh's cattle. Biodegradation by cellulolytic rumen bacteria can be used as a source of cellulolytic bacteria that act to degrade feed fibrous material so as to improve the quality of nutrients and digestibility of feed ingredients at a cheaper price than the use of commercial cellulase enzymes. Materials and Methods: Samples were collected from rumen fluid of Aceh's cattle in Abattoirs (RPH) of Banda Aceh city, Indonesia, isolation, and screening of cellulolytic bacteria were done in Microbiology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia...
December 2017: Veterinary World
https://www.readbyqxmd.com/read/29386234/practice-guidelines-for-clinical-microbiology-laboratories-mycobacteria
#17
REVIEW
Betty A Forbes, Geraldine S Hall, Melissa B Miller, Susan M Novak, Marie-Claire Rowlinson, Max Salfinger, Akos Somoskövi, David M Warshauer, Michael L Wilson
Mycobacteria are the causative organisms for diseases such as tuberculosis (TB), leprosy, Buruli ulcer, and pulmonary nontuberculous mycobacterial disease, to name the most important ones. In 2015, globally, almost 10 million people developed TB, and almost half a million patients suffered from its multidrug-resistant form. In 2016, a total of 9,287 new TB cases were reported in the United States. In 2015, there were 174,608 new case of leprosy worldwide. India, Brazil, and Indonesia reported the most leprosy cases...
April 2018: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/29361269/-the-role-of-pharmacist-in-a-telemedicine-collaboration-in-hemodialysis-water-bacteriological-quality-monitoring
#18
C Letournel, V Montecot
OBJECTIVES: In French health centers, the pharmacist is responsible for the quality of hemodialysis fluids. In an insular hospital, it is difficult to make bacteriological controls because of the lack of an environmental laboratory. Alternative choices of methods must be seek to facilitate water control and ensure the security of hemodialysis for patients. Controlling the microbiological risk is an essential condition for the good operation of a telemedicine partnership in dialysis. METHODS: A review of the different methods that has been tried is presented...
May 2018: Annales Pharmaceutiques Françaises
https://www.readbyqxmd.com/read/29350561/recent-past-present-and-future-of-the-food-microbiome
#19
Francesca De Filippis, Eugenio Parente, Danilo Ercolini
Sequencing technologies have deeply changed our approach to the study of food microbial communities. This review describes recent exploitations of high-throughput sequencing applications to improve our knowledge of food microbial consortia. In the past 10 years, target amplicon sequencing has become routinely used in many food microbiology laboratories, providing a detailed picture of food-associated microbiota. Metagenomics and metatranscriptomics approaches are still underexploited in food microbial ecology, despite their potential to uncover the functionality of complex communities...
March 25, 2018: Annual Review of Food Science and Technology
https://www.readbyqxmd.com/read/29329922/a-multistate-investigation-of-health-care-associated-burkholderia-cepacia-complex-infections-related-to-liquid-docusate-sodium-contamination-january-october-2016
#20
Janet Glowicz, Matthew Crist, Carolyn Gould, Heather Moulton-Meissner, Judith Noble-Wang, Tom J B de Man, K Allison Perry, Zachary Miller, William C Yang, Stephen Langille, Jessica Ross, Bobbiejean Garcia, Janice Kim, Erin Epson, Stephanie Black, Massimo Pacilli, John J LiPuma, Ryan Fagan
BACKGROUND: Outbreaks of health care-associated infections (HAIs) caused by Burkholderia cepacia complex (Bcc) have been associated with medical devices and water-based products. Water is the most common raw ingredient in nonsterile liquid drugs, and the significance of organisms recovered from microbiologic testing during manufacturing is assessed using a risk-based approach. This incident demonstrates that lapses in manufacturing practices and quality control of nonsterile liquid drugs can have serious unintended consequences...
January 9, 2018: American Journal of Infection Control
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