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https://www.readbyqxmd.com/read/29784040/antimicrobial-resistance-profile-of-staphylococcus-aureus-isolated-from-patients-with-infection-at-tikur-anbessa-specialized-hospital-addis-ababa-ethiopia
#1
Sileshi Tadesse, Haile Alemayehu, Admasu Tenna, Getachew Tadesse, Tefaye Sisay Tessema, Workineh Shibeshi, Tadesse Eguale
BACKGROUND: Staphylococcus aureus is one of the major pathogens of public health importance responsible for various forms of infection. Development of resistance to commonly used antimicrobials limited treatment options against infections due to this pathogen. Antimicrobial resistance profile of Staphylococcus aureus isolated from patients with surgical site infection and ear infection and corresponding nasal swab was investigated in Tikur Anbessa Specialized Hospital (TASH), Addis Ababa, Ethiopia...
May 21, 2018: BMC Pharmacology & Toxicology
https://www.readbyqxmd.com/read/29783929/modulation-the-crosstalk-between-tumor-associated-macrophages-and-non-small-cell-lung-cancer-to-inhibit-tumor-migration-and-invasion-by-ginsenoside-rh2
#2
Honglin Li, Nan Huang, Weikang Zhu, Jianchun Wu, Xiaohui Yang, Wenjing Teng, Jianhui Tian, Zhihong Fang, Yingbin Luo, Min Chen, Yan Li
BACKGROUND: Tumor-associated macrophages (TAMs) play a critical role in modulating the tumor microenvironment and promote tumor metastases. Our studies have demonstrated that ginsenoside Rh2 (G-Rh2), a monomeric compound extracted from ginseng, is a promising anti-tumor agent in lung cancer cells. However, it remains unclear whetherG-Rh2 can modulate the differentiation of TAMs and its interaction with tumor microenvironment. In this study, we investigated how G-Rh2 regulates the phenotype of macrophages and affects the migration of non-small cell lung cancer (NSCLC) cells...
May 22, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29782013/mouse-and-human-derived-primary-gastric-epithelial-monolayer-culture-for-the-study-of-regeneration
#3
Emma Teal, Nina G Steele, Jayati Chakrabarti, Loryn Holokai, Yana Zavros
In vitro studies of gastric wound repair typically involves the use of gastric cancer cell lines in a scratch-wound assay of cellular proliferation and migration. One critical limitation of such assays, however, is their homogenous assortment of cellular types. Repair is a complex process which demands the interaction of several cell types. Therefore, to study a culture devoid of cellular subtypes, is a concern that must be overcome if we are to understand the repair process. The gastric organoid model may alleviate this issue whereby the heterogeneous collection of cell types closely reflects that of the gastric epithelium or other native tissues in vivo...
May 7, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29776717/m2-macrophages-promote-wound-induced-hair-neogenesis
#4
Akira Kasuya, Taisuke Ito, Yoshiki Tokura
BACKGROUND: De novo hair regeneration occurs in scars of normal adult mice. This interesting phenomenon is termed as wound-induced hair neogenesis (WIHN). We hypothesized that M2 macrophages are crucially involved in WIHN. OBJECTIVE: To clarify the contribution of M2 macrophages to WIHN. METHOD: We established a mouse model of WIHN. A full thickness skin excision was implemented on the back of C57BL/6 (B6) mice. Newly developing hair follicles were detected by a whole-mount assay...
May 12, 2018: Journal of Dermatological Science
https://www.readbyqxmd.com/read/29774996/-study-of-gonadotropin-releasing-hormone-on-suppressing-migrationg-and-invationg-of-human-nasopharyngeal-carcinoma-cells-cne2
#5
Y H Sun, T T Kong, T T Wang, H F Zhu, T W Zhuang, D P Wang
Objective: The aim of this study is to investigate the effect of gonadotropin releasing hormone (GnRH) on suppressing cell viability, apoptosis, migrationg and invationg of human nasopharyngeal carcinoma cells CNE2. Method: Nasopharyngeal carcinoma tissues and postnasal catarrh tissues were collected, the expression of GnRH positive cells and GnRH mRNA were detected by immunohistochemical staining and qRT-PCR. The human nasopharyngeal carcinoma CNE2 cells and immortalized nasopharyngeal epithelial cell line NP69 were cultured in vitro, and the expression of GnRH positive cells and GnRH mRNA were detected by immunohistochemical staining and qRT-PCR...
June 5, 2017: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
https://www.readbyqxmd.com/read/29774750/time-to-diagnosis-and-treatment-of-surgical-site-infections-in-foot-and-ankle-surgery
#6
Eric S Baranek, Direk Tantigate, Eugene Jang, Justin K Greisberg, J Turner Vosseller
BACKGROUND: The time at which patients typically present with surgical site infections (SSI) following foot and ankle surgery has not been characterized. The primary aim of this study was to quantify the time to definitive treatment of SSIs. METHODS: We performed a retrospective review of 1933 foot and ankle procedures in 1632 patients from 2011 through 2015. Demographic and surgical data were collected. Time to presentation in cases diagnosed with postoperative wound complications or SSIs was analyzed...
May 1, 2018: Foot & Ankle International
https://www.readbyqxmd.com/read/29774178/effects-of-local-application-of-nano-silver-on-osteomyelitis-and-soft-tissue-infections-an-experimental-study-in-rats
#7
Bahattin Kemah, Gökçer Uzer, Yalçın Turhan, Burak Özturan, Bülent Kılıç, Bilge Sümbül Gültepe, Ayşe Bahar Ceyran, Selim Ertürk, Burak Aksoylu, Özlem Şenaydın, Korhan Özkan
Purpose : Bone and soft tissue infections are among the least desired complications after orthopaedic surgery. This study analysed the in vivo effects of the local application of nano-silver particles (AgNPs) [1nm = 1 billionth of a meter] in soft tissue infections. Materials-Method : An experimental osteomyelitis model was formed by inoculating both tibias of 24 rats with methicillin-resistant Staphylococcus aureus . The rats were followed without treatment for 21 days. Blood samples and tibial x-rays at day 21 confirmed the development of infection...
2018: Journal of Bone and Joint Infection
https://www.readbyqxmd.com/read/29772691/periodontal-tissues-maxillary-jaw-bone-and-tooth-regeneration-approaches-from-animal-models-analyses-to-clinical-applications
#8
REVIEW
Fareeha Batool, Marion Strub, Catherine Petit, Isaac Maximiliano Bugueno, Fabien Bornert, François Clauss, Olivier Huck, Sabine Kuchler-Bopp, Nadia Benkirane-Jessel
This review encompasses different pre-clinical bioengineering approaches for periodontal tissues, maxillary jaw bone, and the entire tooth. Moreover, it sheds light on their potential clinical therapeutic applications in the field of regenerative medicine. Herein, the electrospinning method for the synthesis of polycaprolactone (PCL) membranes, that are capable of mimicking the extracellular matrix (ECM), has been described. Furthermore, their functionalization with cyclosporine A (CsA), bone morphogenetic protein-2 (BMP-2), or anti-inflammatory drugs' nanoreservoirs has been demonstrated to induce a localized and targeted action of these molecules after implantation in the maxillary jaw bone...
May 16, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29771377/alternative-splicing-analysis-in-human-monocytes-and-macrophages-reveals-mbnl1-as-major-regulator
#9
Hongfei Liu, Paolo A Lorenzini, Fan Zhang, Shaohai Xu, Mei Su M Wong, Jie Zheng, Xavier Roca
We report the detailed transcriptomic profiles of human innate myeloid cells using RNA sequencing. Monocytes migrate from blood into infected or wounded tissue to differentiate into macrophages, and control inflammation via phagocytosis or cytokine secretion. We differentiated culture primary monocytes with either GM- or M-CSF to obtain pro- or anti-inflammatory macrophages, and respectively activated them with either LPS/IFNγ or anti-inflammatory cytokines. We also treated the THP-1 monocytic cell line with PMA and similar cytokines to mimic differentiation and activation...
May 16, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29768329/retrospective-analysis-of-culture-negative-versus-culture-positive-postoperative-spinal-infections
#10
Jae Chul Lee, Min Jung Baek, Sung-Woo Choi, Soon Hyo Kwon, Kwang-Hyun Kim, Se Yoon Park, Tae Hyong Kim, Suyeon Park, Hae-Dong Jang, Dong-Il Chun, Byung-Joon Shin
Retrospective analysis.This study aimed to investigate the characteristics, clinical features, and outcomes of culture-negative (CN) and culture-positive (CP) postoperative spinal infections (PSIs).Causative organism cultures and the use of adequate antibiotics are essential for treating postoperative spinal wound infections. However, managing infected surgical sites with negative wound culture results is a common clinical problem. Although the outcomes of microbiologically confirmed PSIs have been well studied, the outcomes and clinical characteristics of CN PSIs have not been previously published...
May 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29765411/skin-derived-stem-cells-for-wound-treatment-using-cultured-epidermal-autografts-clinical-applications-and-challenges
#11
REVIEW
Inga Brockmann, Juliet Ehrenpfordt, Tabea Sturmheit, Matthias Brandenburger, Charli Kruse, Marietta Zille, Dorothee Rose, Johannes Boltze
The human skin fulfills important barrier, sensory, and immune functions-all of which contribute significantly to health and organism integrity. Widespread skin damage requires immediate treatment and coverage because massive skin loss fosters the invasion of pathogens, causes critical fluid loss, and may ultimately lead to death. Since the skin is a highly immunocompetent organ, autologous transplants are the only viable approach to permanently close a widespread skin wound. Despite the development of tissue-saving autologous transplantation techniques such as mesh and Meek grafts, treatment options for extensive skin damage remain severely limited...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29764584/-preliminary-study-on-the-effect-of-adipocytes-on-the-biological-behaviors-of-lung-adenocarcinoma-a549-cells-in-tumor-microenvironment
#12
Hang Zhang, Jingjing Li, Yanan Cao, Xiang Dong, Cong Gao, Fanfan Li
BACKGROUND: Adipocytes in the tumor microenvironment may provide the metabolic fuel or signal transduction through media and other means to promote a variety of malignant proliferation and invasion, of tumor cells, but their role in lung cancer progression is still unclear. The purpose of this study was to investigate the effect of adipocytes on lung cancer cell biology. METHODS: 3T3-L1 pre-adipocytes were induced into mature adipocytes. The cell morphology was observed by microscopy and Oil Red O staining...
May 20, 2018: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://www.readbyqxmd.com/read/29764376/methicillin-resistant-staphylococcus-aureus-in-emergency-department-patients-in-the-united-arab-emirates
#13
Muna Al Jalaf, Hanan Fadali, Rasha Alanee, Firas Najjar, Zulfa Al Deesi, Rania M Seliem, Eric J Nilles
BACKGROUND: Since the 1990s, community-associated methicillin resistant staphylococcus aureus (CA-MRSA) has emerged as an important global cause of skin and soft tissue infections. Little is known about the epidemiology of this pathogen in the Middle East. METHODS: We conducted a prospective observational study in a single large teaching hospital in Dubai to identify the incidence of community-acquired methicillin resistant staphylococcus aureus (MRSA) among ambulatory patients presenting with purulent skin and soft tissue infections...
May 15, 2018: BMC Emergency Medicine
https://www.readbyqxmd.com/read/29763936/synergistic-inhibition-of-thalidomide-and-icotinib-on-human-non-small-cell-lung-carcinomas-through-erk-and-akt-signaling
#14
Xiang Sun, Yang Xu, Yi Wang, Qian Chen, Liu Liu, Yangyi Bao
BACKGROUND Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) have been widely used in the treatment of non-small cell lung cancer (NSCLC) patients with sensitive EGFR mutations. However, the survival of patients with EGFR-TKI administration is limited by the inevitable development of acquired drug resistance. Recently, multi-targeted drugs combination has been shown to be a promising strategy to improve the efficacy of EGFR-TKI treatment and enable the reduction of drug resistance in NSCLC...
May 15, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29757663/bicipital-tendinopathy-and-carpal-valgus-following-a-distant-direct-thoracoabdominal-skin-flap-in-a-german-shepherd-with-necrotizing-fasciitis
#15
Benjamin Mielke, Nicola Kulendra, Zoe Halfacree, Elvin Kulendra
A 4 mo old German shepherd dog was presented following an acute onset lameness and swelling of the thoracic limb. Clinical signs, surgical findings, and culture results were consistent with a diagnosis of necrotizing fasciitis. Initial management consisted of extensive surgical debridement, wound lavage, and bandaging. Surgical closure was achieved with an axillary fold flap and distant direct single-pedicle thoracoabdominal flap. Three mo later, following deterioration of the lameness that was associated with carpal valgus, carpal subluxation, and tendinopathy of the distal radial insertion of the biceps brachii tendon, a closing wedge pancarpal arthrodesis and tenotomy of the biceps brachii tendon was performed...
May 14, 2018: Journal of the American Animal Hospital Association
https://www.readbyqxmd.com/read/29756075/alterations-in-amino-acid-metabolism-during-growth-by-staphylococcus-aureus-following-exposure-to-h-2-o-2-a-multifactorial-approach
#16
Grace R Murphy, R Hugh Dunstan, Margaret M Macdonald, Johan Gottfries, Tim K Roberts
Temperature and pH are known to vary in a wound site due to the immune response and subsequent healing processes. This study used a multifactorial design to examine the cellular responses of Staphylococcus aureus to hydrogen peroxide (0-100 mM) when bacteria were grown in temperatures of 37 ± 2 °C and pH 7 ± 1, conditions potentially encountered in wound sites. A centroid sample was included in the design which represented the mid-point values of all three environmental parameters (37 °C, pH 7, 50 mM H2 O2 )...
May 2018: Heliyon
https://www.readbyqxmd.com/read/29755898/an-unusual-presentation-of-kikuchi-fujimoto-disease-with-recurrent-subdural-effusion
#17
Sara Shahid, Syed H Alam, Indira Hadley
A 24-year-old man complained of a right temporal headache for four weeks. The patient denied any trauma or previous anticoagulation use. He also reported tender right facial swelling. His physical exam was unrevealing except for right cranial nerve (CN) VI palsy, right parotid enlargement, and cervical adenopathy. Laboratory findings were significant for mild leukopenia at 3300 cells/uL. The computed tomography (CT) scan obtained showed a chronic left subdural effusion with a 4 mm midline shift and confirmed right parotid enlargement and cervical lymphadenopathy...
March 10, 2018: Curēus
https://www.readbyqxmd.com/read/29755597/5-fluorouracil-may-enrich-cancer-stem-cells-in-canine-mammary-tumor-cells-in-vitro
#18
Bin Zhou, Yipeng Jin, Di Zhang, Degui Lin
Mammary gland carcinomas are the most common neoplasms in women and unsterilized female dogs. Owing to the existence of cancer stem cells (CSCs), chemotherapy is not able to cure these types of diseases completely. A number of studies have demonstrated that CSCs are resistant to chemotherapeutic drugs, but whether canine mammary tumor cells that have acquired resistance to 5-fluorouracil (5-FU) exhibited properties of CSCs remains unknown. The aim of the present study was to investigate whether 5-fluorouracil-resistant canine mammary tumor cells exhibited properties of CSCs...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29755530/the-effect-of-age-on-the-regenerative-potential-of-human-eyelid-adipose-derived-stem-cells
#19
Mingqi Zhang, Zhuoshi Wang, Yan Zhao, Lirong Zhang, Ling Xu, Liu Cao, Wei He
Human eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal stem cells, which are derived from neuroectoderm and potentially applied in the tissue regeneration and cell therapies. Based on the prevalence of blepharoplasty in Asia and the availability of HEASCs, we investigated the effect of donor age on their characteristics and regenerative potential of HEASCs in vitro. The HEASCs were isolated from patients of three groups: (1) <20 years ( n = 4), (2) >20 years, <45 years ( n = 5), and (3) >55 years ( n = 4)...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29753580/acceleration-of-diabetic-wound-healing-with-adipose-derived-stem-cells-endothelial-differentiated-stem-cells-and-topical-conditioned-medium-therapy-in-a-swine-model
#20
Robin F Irons, Kevin W Cahill, Deviney A Rattigan, Joseph H Marcotte, Marc W Fromer, Shaohua Chang, Ping Zhang, Eric M Behling, Kathryn C Behling, Francis J Caputo
OBJECTIVE: The purpose of our study was to investigate the effect of adipose-derived stem cells (ASCs), endothelial-differentiated ASCs (EC/ASCs), and various conditioned media (CM) on wound healing in a diabetic swine model. We hypothesized that ASC-based therapies would accelerate wound healing. METHODS: Diabetes was induced in four Yorkshire swine through intravenous injection of streptozotocin. ASCs were harvested from flank fat and cultured in either M199 or EGM-2 medium...
May 9, 2018: Journal of Vascular Surgery
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