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https://www.readbyqxmd.com/read/29148603/mcp2-activates-nf-%C3%AE%C2%BAb-signaling-pathway-promoting-the-migration-and-invasion-of-escc-cells
#1
Jian Zhou, Shutao Zheng, Tao Liu, Qing Liu, Yumei Chen, Doudou Tan, Rong Ma, Xiaomei Lu
MCP2, aliased CCL8, has been suggested to be implicated in the metastasis of cancer cells; however no direct evidence has been established in esophageal squamous cell carcinoma (ESCC). In our present study, to investigate the role MCP2 played in the metastasis of ESCC cells; in vitro cell co-culture system was established. Wound-healing and Transwell assays were used to evaluate the migratory and invasive variation of ESCC cells before and after treatment with recombinant human MCP2. It was shown that MCP2 was able to activate the NF-κB signaling pathway inducing the epithelial-mesenchymal transition (EMT) and promoting the migration and invasion of ESCC cells in vitro...
November 17, 2017: Cell Biology International
https://www.readbyqxmd.com/read/29147946/a-case-report-of-the-first-application-of-culture-epithelial-autograft-jace-%C3%A2-for-giant-congenital-melanocytic-nevus-after-its-approval-in-japan
#2
Naoki Morimoto, Natsuko Kakudo, Ayako Kako, Keiko Nishimura, Toshihito Mitsui, Ryohei Miyake, Atsuyuki Kuro, Masakatsu Hihara, Kenji Kusumoto
In Japan, the JACE(®) cultured epidermal autograft (CEA) was approved and covered by public healthcare insurance for use in the treatment of giant congenital melanocytic nevus (GCMN) in 2016. We herein report the results of the application of JACE(®) after curettage and Q-switched ruby laser therapy. The current patient was the first patient with GCMN to be treated with JACE(®) since its approval. A 3-month-old girl had a hairy GCMN of 9.5 cm in diameter from her cheek to her temple on the left side of her face...
November 17, 2017: Journal of Artificial Organs: the Official Journal of the Japanese Society for Artificial Organs
https://www.readbyqxmd.com/read/29147558/discovery-and-design-of-self-assembling-peptides
#3
REVIEW
Shuguang Zhang
Peptides are ubiquitous in nature and useful in many fields, from agriculture as pesticides, in medicine as antibacterial and antifungal drugs founded in the innate immune systems, to medicinal chemistry as hormones. However, the concept of peptides as materials was not recognized until 1990 when a self-assembling peptide as a repeating segment in a yeast protein was serendipitously discovered. Peptide materials are so called because they have bona fide materials property and are made from simple amino acids with well-ordered nanostructures under physiological conditions...
December 6, 2017: Interface Focus
https://www.readbyqxmd.com/read/29144448/regeneration-of-the-entire-human-epidermis-using-transgenic-stem-cells
#4
Tobias Hirsch, Tobias Rothoeft, Norbert Teig, Johann W Bauer, Graziella Pellegrini, Laura De Rosa, Davide Scaglione, Julia Reichelt, Alfred Klausegger, Daniela Kneisz, Oriana Romano, Alessia Secone Seconetti, Roberta Contin, Elena Enzo, Irena Jurman, Sonia Carulli, Frank Jacobsen, Thomas Luecke, Marcus Lehnhardt, Meike Fischer, Maximilian Kueckelhaus, Daniela Quaglino, Michele Morgante, Silvio Bicciato, Sergio Bondanza, Michele De Luca
Junctional epidermolysis bullosa (JEB) is a severe and often lethal genetic disease caused by mutations in genes encoding the basement membrane component laminin-332. Surviving patients with JEB develop chronic wounds to the skin and mucosa, which impair their quality of life and lead to skin cancer. Here we show that autologous transgenic keratinocyte cultures regenerated an entire, fully functional epidermis on a seven-year-old child suffering from a devastating, life-threatening form of JEB. The proviral integration pattern was maintained in vivo and epidermal renewal did not cause any clonal selection...
November 16, 2017: Nature
https://www.readbyqxmd.com/read/29143889/beyond-pathologizing-harm-understanding-ptsd-in-the-context-of-war-experience
#5
Patricia Benner, Jodi Halpern, Deborah R Gordon, Catherine Long Popell, Patricia W Kelley
An alternative to objectifying approaches to understanding Post-traumatic Stress Disorder (PTSD) grounded in hermeneutic phenomenology is presented. Nurses who provided care for soldiers injured in the Iraq and Afghanistan wars, and sixty-seven wounded male servicemen in the rehabilitation phase of their recovery were interviewed. PTSD is the one major psychiatric diagnosis where social causation is established, yet PTSD is predominantly viewed in terms of the usual neuro-physiological causal models with traumatic social events viewed as pathogens with dose related effects...
November 16, 2017: Journal of Medical Humanities
https://www.readbyqxmd.com/read/29140838/assessing-specimens-of-devitalized-tissue-in-chronic-sacral-pressure-ulcers-a-pilot-study
#6
Juliany Lino Gomes Silva, Andressa Coope, Eliana Pereira de Araújo, Licio A Velloso, Mário José A Saad, Maria Helena Melo Lima
Pressure ulcers (PrUs)* are chronic wounds caused by a combination of factors, such as repetitive ischemia perfusion injury, bacterial colonization of the wound bed, local tissue hypoxia, or an altered cellular and systemic stress response. The objectives of this study were to analyze fragments of devitalized tissue of Stages III and IV PrUs and compare them with healthy tissue to evaluate the expression of the genes involved in wound inflammation. Samples of healthy skin from patients undergoing plastic surgery (n = 3) were collected, as well as from patients with devitalized tissue from Stages III and IV PrUs (n = 3) by means of sharp debridement...
December 2017: Advances in Skin & Wound Care
https://www.readbyqxmd.com/read/29140837/disruption-of-biofilms-and-neutralization-of-bacteria-using-hypochlorous-acid-solution-an-in-vivo-and-in-vitro-evaluation
#7
Anna Day, Abdulnaser Alkhalil, Bonnie C Carney, Hilary N Hoffman, Lauren T Moffatt, Jeffrey W Shupp
OBJECTIVES: The aims of this study were to assess the effectiveness of a hypochlorous acid-based wound cleanser (Vashe Wound Solution [VWS], SteadMed Medical, Fort Worth, Texas) in disrupting methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa biofilms relative to other cleansers using an in vitro collagen biofilm model and to evaluate cleansers' cytotoxicity. The bioburden reduction of venous stasis wounds by VWS and another cleanser was evaluated. METHODS: Plates coated with collagen films incubated with active bacteria cultures to yield biofilm mimics were treated with VWS, 1% and 10% povidone-iodine (PI), 0...
December 2017: Advances in Skin & Wound Care
https://www.readbyqxmd.com/read/29140388/titanium-activates-the-dna-damage-response-pathway-in-oral-epithelial-cells-a-pilot-study
#8
Fernando Suárez-López Del Amo, Ivan Rudek, Vivian Petersen Wagner, Manoela D Martins, Francisco O'Valle, Pablo Galindo-Moreno, William V Giannobile, Hom-Lay Wang, Rogerio M Castilho
PURPOSE: To evaluate the effect of titanium (Ti) particles on oral epithelial cell homeostasis and the potential of dental implants to release Ti debris upon insertion. MATERIALS AND METHODS: Dental implants with varying surface treatments were employed to determine the feasibility of particle release during implant placement as well as the impact of free Ti debris on oral epithelial cells. Ti particles derived from implant surfaces were isolated and cultured in direct contact with normal oral epithelial cells for 48 hours...
November 2017: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/29138638/tissue-source-and-cell-expansion-condition-influence-phenotypic-changes-of-adipose-derived-stem-cells
#9
Lauren H Mangum, Shanmugasundaram Natesan, Randolph Stone, Nicole L Wrice, David A Larson, Kyle F Florell, Barbara A Christy, Maryanne C Herzig, Andrew P Cap, Robert J Christy
Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In this study, adipogenic and osteogenic differentiation in media supplemented with 10% FBS or 10% hPL was compared in human ASCs derived from abdominoplasty (HAP) or from adipose associated with debrided burned skin (BH)...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29138622/antimicrobial-efficacy-of-penicillium-amestolkiae-elv609-extract-treated-cotton-fabric-for-diabetic-wound-care
#10
Nur Amiera Syuhada Binti Rozman, Nurhanis Syafiqah Binti Mohd Nor Hamin, Leong Chean Ring, Tan Wen Nee, Mahfuzah Binti Mustapha, Tong Woei Yenn
Diabetes mellitus is a chronic disorder which affects millions of population worldwide. Global estimates published in 2010 reported the world diabetic prevalence as 6.4%, affecting 285 million adults. Foot ulceration and wound infection are major forms of disabilities arising from diabetic diseases. This study was aimed to develop a natural antimicrobial finishing on medical grade textile that meets American Association of Textiles Chemists and Colorists (AATCC) standard. The textile samples were finished with the ethanolic extract of Penicillium amestolkiae elv609, an endophytic fungus isolated from Orthosiphon stamineus Benth (common name: cat's whiskers)...
September 2017: Mycobiology
https://www.readbyqxmd.com/read/29136259/risk-factors-for-surgical-site-infections-in-dermatological-surgery
#11
Xiaomeng Liu, Melissa Sprengers, Patty J Nelemans, Klara Mosterd, Nicole W J Kelleners-Smeets
Current literature on risk factors for surgical site infection (SSI) in dermatological surgery in the absence of antibiotic prophylaxis is limited. The aim of this study was to retrospectively evaluate patients presenting for dermatological surgery. A total of 1,977 procedures were reviewed. SSI was clinically suspected in 79 (4.0%) patients and confirmed by culture in 38 (1.9%). Using the strictest definition of SSI (clinical symptoms with positive culture) significantly higher risk of SSI was found for location on the ear (odds ratio (OR) 6...
November 14, 2017: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/29135121/hepcidin-and-iron-metabolism-in-the-pathogenesis-of-prostate-cancer
#12
Feng Wang, An Liu, Ruyu Bai, Binbin Zhang, Yongsheng Jin, Wei Guo, Yi Li, Jixue Gao, Longqiang Liu
PURPOSE: To investigate the relationship between Hepcidin and iron metabolism, and cell proliferation, migration, and apoptosis in prostate cancer cells. METHODS: PC3 prostate cancer cells were cultured in vitro and divided into the control group, Hepcidin overexpression group, and Hepcidin low expression group. Prostate specific antigen (PSA) and soluble transferrin receptor (sTfR) levels were measured by ELISA. The levels of the Hepcidin receptor membrane transporter, Ferroportin, were determined by Western blot...
September 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29134714/identification-of-perivascular-and-stromal-mesenchymal-stem-progenitor-cells-in-porcine-endometrium
#13
J Wiater, M Niedziela, A Posmysz, K Wartalski, B Gajda, Z Smorąg, Z Rajfur, J Karasiński
Mammalian uterus contains a population of mesenchymal stem/progenitor cells that likely contribute to endometrial regeneration during each reproductive cycle. In human and mouse, they reside in perivascular, epithelial and stromal compartments of the endometrial functionalis and basalis. Here, we aimed to identify tissue resident cells expressing mesenchymal stem cell markers CD29, CD44, CD90, CD105, CD140b and CD146 in the porcine endometrium. We used single immunofluorescence and Western blotting. Each of these markers was detected in small cells surrounding endometrial blood vessels...
November 14, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29131990/non-thermal-near-infrared-exposure-photobiomodulates-cellular-responses-to-ionizing-radiation-in-human-full-thickness-skin-models
#14
Anke König, Nadja Zöller, Stefan Kippenberger, August Bernd, Roland Kaufmann, Paul G Layer, Anja Heselich
Ionizing and near-infrared radiation are both part of the therapeutic spectrum in cancer treatment. During cancer therapy ionizing radiation is typically used for non-invasive reduction of malignant tissue, while near-infrared photobiomodulation is utilized in palliative medical approaches, e.g. for pain reduction or impairment of wound healing. Furthermore, near-infrared is part of the solar wavelength spectrum. A combined exposure of these two irradiation qualities - either intentionally during medical treatment or unintentionally due to solar exposure - is therefore presumable for cancer patients...
November 10, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29127427/amniotic-membrane-promotes-focal-adhesion-remodeling-to-stimulate-cell-migration
#15
Ángel Bernabé-García, Sergio Liarte, Jose M Moraleda, Gregorio Castellanos, Francisco J Nicolás
During wound healing, the migration of keratinocytes onto newly restored extracellular matrix aims to reestablish continuity of the epidermis. The application of amniotic membrane (AM) to chronic, deep traumatic, non-healing wounds has proven successful at stimulating re-epithelialization. When applied on epithelial cell cultures, AM activates extracellular signal-regulated kinases 1/2 (ERK1/2) and c-Jun N-terminal kinases 1/2 (JNK1/2), with the overexpression and phosphorylation of c-Jun along the wound edge...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29126993/trophic-effect-of-pacap-on-human-corneal-endothelium
#16
Grazia Maugeri, Antonio Longo, Agata Grazia D'Amico, Daniela Maria Rasà, Michele Reibaldi, Andrea Russo, Vincenza Bonfiglio, Teresio Avitabile, Velia D'Agata
Cornea's posterior surface includes endothelium maintaining stromal hydration and clarity. Due to their limited proliferative capability, the loss of endothelial cells can outcome in permanent opacity. In the last years, different studies have demonstrated the protective effect of pituitary adenylate cyclase-activating polypeptide (PACAP) in different ocular diseases. However, its role on human corneal endothelial cells (HCECs) has not been investigated, yet. Here, we have developed a culture protocol to differentiate HCECs from donor's cornea...
November 8, 2017: Peptides
https://www.readbyqxmd.com/read/29124834/prevalence-and-characterization-of-staphylococcus-aureus-causing-community-acquired-skin-and-soft-tissue-infections-on-java-and-bali-indonesia
#17
Dewi Santosaningsih, Sanarto Santoso, Nanik Setijowati, Harun A Rasyid, Nyoman S Budayanti, Ketut Suata, Dicky B Widhyatmoko, Priyo B Purwono, Kuntaman Kuntaman, Damayanti Damayanti, Cita R S Prakoeswa, Mitchell Laurens, Josephine W I van Nierop, Geraldine L Nanninga, Neline Oudenes, Michelle de Regt, Susan V Snijders, Henri A Verbrugh, Juliëtte A Severin
OBJECTIVES: To define the role of Staphylococcus aureus in community settings among patients with skin and soft tissue infections (SSTI) in Indonesia. METHODS: S. aureus were cultured from anterior nares, throat, and wounds of 567 ambulatory patients presenting with SSTI. The mecA gene and genes encoding Panton-Valentine leukocidin (PVL; lukF-PV and lukS-PV) and exfoliative toxin (ET; eta and etb) were determined by PCR. Clonal relatedness among methicillin-resistant S...
November 10, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/29119225/piperlongumine-inhibits-the-proliferation-migration-and-invasion-of-fibroblast-like-synoviocytes-from-patients-with-rheumatoid-arthritis
#18
Siqi Xu, Youjun Xiao, Shan Zeng, Yaoyao Zou, Qian Qiu, Mingcheng Huang, Zhongping Zhan, Liuqin Liang, Xiuyan Yang, Hanshi Xu
OBJECTIVES: Recent studies have indicated that piperlongumine (PLM) may exert anti-inflammatory effects. In the present study, we determined the effect of PLM on the proliferation, apoptosis, migration and invasion of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA) (referred to herein as RA FLS). We further explored the mechanisms by which the studied compound inhibits the functions of RA FLS. METHODS: RA FLS viability and apoptosis were tested using MTT and Annexin V/PI assays, respectively...
November 8, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/29118260/circadian-actin-dynamics-drive-rhythmic-fibroblast-mobilization-during-wound-healing
#19
Nathaniel P Hoyle, Estere Seinkmane, Marrit Putker, Kevin A Feeney, Toke P Krogager, Johanna E Chesham, Liam K Bray, Justyn M Thomas, Ken Dunn, John Blaikley, John S O'Neill
Fibroblasts are primary cellular protagonists of wound healing. They also exhibit circadian timekeeping, which imparts an approximately 24-hour rhythm to their biological function. We interrogated the functional consequences of the cell-autonomous clockwork in fibroblasts using a proteome-wide screen for rhythmically expressed proteins. We observed temporal coordination of actin regulators that drives cell-intrinsic rhythms in actin dynamics. In consequence, the cellular clock modulates the efficiency of actin-dependent processes such as cell migration and adhesion, which ultimately affect the efficacy of wound healing...
November 8, 2017: Science Translational Medicine
https://www.readbyqxmd.com/read/29115647/clinical-infection-in-burn-patients-and-its-consequences
#20
Cem Emir Güldoğan, Murat Kendirci, Deniz Tikici, Emre Gündoğdu, Ahmet Çınar Yastı
BACKGROUND: Burn injuries facilitate invasive infections and sepsis not only by destroying the continuity of the protective skin barrier but also through systemic effects. The burn wound, blood, and urine samples are frequently cultured to determine the pathogen agent. The aim of this study was to analyze pathogen growth in patients' cultures confirmed as "infection positive" by the hospital Infection Control Committee and to assess the clinical implications of these growths. METHODS: Hospitalized patients included in the study were those with a total burned body surface area of >10% and "presence of infection" confirmed by the Infection Control Committee...
November 2017: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
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