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https://www.readbyqxmd.com/read/28542559/fluvastatin-inhibits-age-induced-cell-proliferation-and-migration-via-an-erk5-dependent-nrf2-pathway-in-vascular-smooth-muscle-cells
#1
Ae-Rang Hwang, Jung-Hwa Han, Jae Hyang Lim, Young Jin Kang, Chang-Hoon Woo
Advanced glycation endproduct (AGE)-induced vascular smooth muscle cell (VSMC) proliferation and reactive oxygen species (ROS) production are emerging as important mechanisms of diabetic vasculopathy, but little is known about the molecular mechanism responsible for the antioxidative effects of statins on AGEs. It has been reported that statins exert pleiotropic effects on the cardiovascular system due to decreases in AGE-induced cell proliferation, migration, and vascular inflammation. Thus, in the present study, the authors investigated the molecular mechanism by which statins decrease AGE-induced cell proliferation and VSMC migration...
2017: PloS One
https://www.readbyqxmd.com/read/28542491/the-face-of-war-trauma-analysis-of-a-mass-grave-from-the-battle-of-l%C3%A3-tzen-1632
#2
Nicole Nicklisch, Frank Ramsthaler, Harald Meller, Susanne Friederich, Kurt W Alt
Contemporary accounts of battles are often incomplete or even erroneous because they reflect the-often biased-viewpoints of the authors. Battlefield archaeology faces the task of compiling an historical analysis of a battle and of gathering all the available facts. Besides cultural historical evidence and artefacts, the human remains of those who have fallen in battle also provide invaluable information. In studying mass graves from a military context, the injury types and patterns are significant. They allow us to reconstruct the circumstances surrounding the soldiers' deaths and provide information on the hostilities that occurred on the battlefield...
2017: PloS One
https://www.readbyqxmd.com/read/28542434/interleukin-6-stimulates-akt-and-p38-mapk-phosphorylation-and-fibroblast-migration-in-non-diabetic-but-not-diabetic-mice
#3
Tsubame Nishikai-Yan Shen, Shigeyuki Kanazawa, Makiko Kado, Kayoko Okada, Lin Luo, Ayato Hayashi, Hiroshi Mizuno, Rica Tanaka
Persistent inflammatory environment and abnormal macrophage activation are characteristics of chronic diabetic wounds. Here, we attempted to characterize the differences in macrophage activation and temporal variations in cytokine expression in diabetic and non-diabetic wounds, with a focus on interleukin (IL)-6 mRNA expression and the p38 MAPK and PI3K/Akt signaling pathways. Cutaneous wound closure, CD68- and arginase-1 (Arg-1)-expressing macrophages, and cytokine mRNA expression were examined in non-diabetic and streptozotocin-induced type 1 diabetic mice at different time points after injury...
2017: PloS One
https://www.readbyqxmd.com/read/28542370/upregulation-of-tlr4-via-pkc-activation-contributes-to-impaired-wound-healing-in-high-glucose-treated-kidney-proximal-tubular-cells
#4
Jianping Peng, Hang Zheng, Xia Wang, Zhixiang Cheng
Acute kidney injury (AKI) leads to a worse prognosis in diabetic patients compared with prognoses in non-diabetic patients, but whether and how diabetes affects kidney repair after AKI remains unknown. Here, we used scratch-wound healing and transwell migration models to examine whether and how wound healing is affected by high glucose levels in cultured kidney proximal tubular cells (RPTC). The results show that scratch-wound healing and transwell migration were significantly slower in high-glucose-treated kidney tubular cells (30 mM glucose) than in low-glucose-treated cells (5...
2017: PloS One
https://www.readbyqxmd.com/read/28540567/-the-significance-of-biofilm-for-the-treatment-of-infections-in-orthopedic-surgery-2017-update
#5
REVIEW
C Scheuermann-Poley, C Wagner, J Hoffmann, A Moter, C Willy
BACKGROUND: The increase in endoprosthetic and osteosynthetic surgical treatment is associated with a simultaneous increase in implant-associated infections (surgical site infections, SSI). Biofilms appear to play a significant role in the diagnosis and treatment of these infections and heavily contaminated wounds. This article aims to provide a current overview of biofilm and its relevance in orthopedic surgery. MATERIALS AND METHODS: A computer-assisted literature search of MedLine (PubMed) was performed using key word combinations with "biofilm" (as of March 2017)...
May 24, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/28540528/sema3a-promotes-the-resolution-of-cardiac-inflammation-after-myocardial-infarction
#6
Marieke Rienks, Paolo Carai, Nicole Bitsch, Mark Schellings, Maarten Vanhaverbeke, Johan Verjans, Ilona Cuijpers, Stephane Heymans, Anna Papageorgiou
Optimal healing after myocardial infarction requires not only the induction of inflammation, but also its timely resolution. In patients, 30 days post myocardial infarction, circulating monocytes have increased expression of Semaphorin3A (Sema3A) as compared to directly after admission. This increased expression coincides with increased expression of Cx3CR1-a marker of non-classical monocytes that are important for immune resolution hence proper wound healing. In mice, the expression of Sema3A also increases in response to myocardial ischemia being expressed by infiltrating leukocytes...
July 2017: Basic Research in Cardiology
https://www.readbyqxmd.com/read/28540300/extracellular-signal-regulated-kinase-5-is-required-for-low-concentration-h2o2-induced-angiogenesis-of-human-umbilical-vein-endothelial-cells
#7
Shan Jiang, Dongxin Zhang, Hong Huang, Yonghong Lei, Yan Han, Weidong Han
Background. The aim of this study was to assess the effects of low concentrations of H2O2 on angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro and explore the underlying mechanisms. Methods. HUVECs were cultured and stimulated with different concentrations of H2O2. Flow cytometric analysis was used to select an optimal concentration of H2O2 for the following experiments. Cell proliferation, migration, and tubule formation were evaluated by Cell Counting Kit-8 (CCK-8) assays, scratch wound assays, and Matrigel tubule formation assays, respectively...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28540152/epidemiological-clinical-and-microbiological-characteristics-of-patients-with-post-traumatic-osteomyelitis-of-limb-fractures-in-southwest-china-a-hospital-based-study
#8
Jiachen Peng, Youliang Ren, Wenbin He, Zhengdao Li, Jin Yang, Yi Liu, Zhonghui Zheng, Stephen L Kates, Edward M Schwarz, Chao Xie, Youjia Xu
Objective To determine the epidemiological, clinical and microbiological characteristics, of patients with post-traumatic osteomyelitis of extremity fractures, and provide evidence-based guidelines for early diagnosis and treatment, including empiric antibiotic therapy. Methods Human subject research was performed using institutional review board approved protocols. A retrospective chart review was conducted on 5,368 patients diagnosed with extremity traumatic fractures from January 1, 2012 to December 31, 2015, to identify osteomyelitis patients...
2017: J Bone Jt Infect
https://www.readbyqxmd.com/read/28538648/less-is-more-antibiotic-duration-and-outcomes-in-fournier-s-gangrene
#9
Margaret Lauerman, Olga Kolesnik, Kinjal Sethuraman, Ronald Rabinowitz, Manjari Joshi, Emily Clark, Deborah Stein, Thomas Scalea, Sharon Henry
BACKGROUND: Antibiotic management of Fournier's gangrene (FG) is without evidence-based guidelines, and is based on expert opinion. The effect of duration of antibiotic therapy on outcomes in FG is unknown. METHODS: A retrospective review was performed of FG patients from 2012-2015 at a single institution. Patients were managed by our institutional practice of complete primary wound closure as possible, with antibiotic duration according to physician judgement. Patients were stratified into multiple durations of antibiotic administration...
May 22, 2017: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/28536636/a-simple-and-effective-protocol-for-fast-isolation-of-human-tenon-s-fibroblasts-from-a-single-trabeculectomy-biopsy-a-comparison-of-cell-behaviour-in-different-culture-media
#10
Agata Przekora, Tomasz Zarnowski, Grazyna Ginalska
BACKGROUND: Human Tenon's fibroblasts (HTFs) play a crucial role in wound healing. They cause postoperative scarring of the filtering bleb and are thus responsible for trabeculectomy failure. This study aimed to find an effective and fast protocol for HTF isolation from trabeculectomy biopsies. The protocol was compared with the commonly recommended HTF isolation procedure, which uses Dulbecco's modified Eagle's medium (DMEM). We used Eagle's minimum essential medium (EMEM) enriched with fibroblast growth factor (FGF), which selectively promoted the proliferation of HTF cells...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28532438/the-effect-of-color-type-on-early-wound-healing-in-farmed-mink-neovison-vison
#11
A Jespersen, H E Jensen, J F Agger, P M H Heegaard, P Damborg, B Aalbæk, A S Hammer
BACKGROUND: Individual differences of mink, including color type, are speculated to affect the course of wound healing, thereby impacting wound assessment and management on the farms, as well as the assessment of wounds in forensic cases. In this study, we examined the effect of color type on early wound healing in farmed mink. Full thickness excisional wounds (2 × 2 cm) were made on the back in 18 mink of the color types Brown, Silverblue and Blue Iris. Gross and microscopic pathology of the wounds was evaluated 2 days post-wounding together with degree of wound size reduction, presence of bacteria and blood analyses...
May 22, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28532022/a-novel-grapheme-oxide-modified-collagen-chitosan-bio-film-for-controlled-growth-factor-release-in-wound-healing-applications
#12
Ting Liu, Weihua Dan, Nianhua Dan, Xinhua Liu, Xuexu Liu, Xu Peng
Collagen-chitosan composite film modified with grapheme oxide (GO) and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), termed CC-G-E film, was loaded with basic fibroblast growth factor (bFGF) as the development of an efficacious wound healing device. In this study we report a novel drug delivery system that prevents the initial burst release and loss of bioactivity of drugs in vitro and in vivo applications. The results showed that CC-G-E film possessed improved thermal stability and a higher rate of crosslinking with increased mechanical properties when the dosage of GO was between 0...
August 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28530199/synthesis-of-ag-nps-impregnated-cellulose-composite-material-its-possible-role-in-wound-healing-and-photocatalysis
#13
Attarad Ali, Ihsan Ul Haq, Javeed Akhtar, Muhammad Sher, Naveed Ahmed, Muhammad Zia
Cellulose is the natural biopolymer normally used as supporting agent with enhanced applicability and properties. In present study, cellulose isolated from citrus waste is used for silver nanoparticles (Ag-NPs) impregnation by a simple and reproducible method. The Ag-NPs fabricated cellulose (Ag-Cel) was characterised by powder X-rays diffraction, Fortier transform infrared spectroscopy and scanning electron microscopy. The thermal stability was studied by thermo-gravimetric analysis. The antibacterial activity performed by disc diffusion assay reveals good zone of inhibition against Staphylococcus aureus and Escherichia coli by Ag-Cel as compared Ag-NPs...
June 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/28528048/real-time-autofluorescence-imaging-to-diagnose-lvad-driveline-infections
#14
Jack B Keenan, Taufiek Konrad Rajab, David G Armstrong, Zain Khalpey
A 64-year-old man experienced a driveline infection that was treated with serial debridements and antibiotics. When the wound clinically appeared ready for closure, a handheld fluorescence imaging device still revealed a margin of red fluorescence around the wound edges consistent with a subclinical infection. Therefore, a wider margin was made and additional specimens for wound culture were taken, which demonstrated a vancomycin-resistant enterococcal infection. The autofluorescence signals of common bacteria can be detected with a fluorescence camera in subclinical wound infections without clinical signs...
June 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/28526307/risk-factors-of-cardiac-device-infection-glove-contamination-during-device-procedures
#15
Isabella Kozon, Sam Riahi, Søren Lundbye-Christensen, Anna Margrethe Thøgersen, Tove Ejlertsen, Dorthe Aaen, Kirsten I Paulsen, Søren Hjortshøj
BACKGROUND: Infections in cardiac implantable electronic devices (CIEDs) constitute a serious complication. We sought to identify contamination of gloves before handling the device in primary and replacement CIED procedures. METHODS: Two groups of 30 patients underwent primary CIED implantation or replacement. Before the device entered the surgical field, surgeon and assistant imprinted their outer gloves on aerobe and anaerobe agar plates, and a wound swab was performed...
May 16, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28526303/the-use-of-biobrane-%C3%A2-for-wound-coverage-in-stevens-johnson-syndrome-and-toxic-epidermal-necrolysis
#16
Alan D Rogers, Erin Blackport, Robert Cartotto
INTRODUCTION: Published experience describing the use of Biobrane(®) for wound management in Stevens-Johnson Syndrome and Toxic Epidermal Necrolysis (SJS-TEN) is limited to case reports and case series involving ten or fewer patients. We have used Biobrane(®) in the care of SJS-TEN since 2000, and the purpose of this study was to review our experience with the application of Biobrane(®) for wound coverage in SJS-TEN. METHODS: A retrospective review of all cases of SJS-TEN admitted to an adult regional ABA-verified burn center between January 1, 2000 and June 1, 2015 was conducted...
May 16, 2017: Burns: Journal of the International Society for Burn Injuries
https://www.readbyqxmd.com/read/28526214/high-glucose-environment-induced-intracellular-o-glcnac-glycosylation-and-reduced-galectin-7-expression-in-keratinocytes-implications-on-impaired-diabetic-wound-healing
#17
Shu-Mei Huang, Ching-Shuang Wu, Min-Hsi Chiu, Hui-Jun Yang, Gwo-Shing Chen, Cheng-Che E Lan
BACKGROUND: Diabetes is an important global health issue due to its increasing prevalence and association with various complications. Impaired wound healing is a serious complication associated with diabetes that frequently results in infection and amputation. Galectin-7 (Gal-7) has been reported to play an important role during skin wound healing. Previously, we had demonstrated that high glucose environment alters physiologic functions of keratinocytes and contributes to impaired wound healing in diabetic condition...
April 30, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28517972/glycated-collagen-stimulates-the-differentiation-of-gingival-myofibroblasts
#18
Ignacio N Retamal, Romina Hernández, Francisco Melo, Paulina Zapata, Constanza Martínez, Jorge Martínez, Patricio C Smith
BACKGROUND: Glucose-derived metabolites may alter the structure and biological properties of important proteins in the periodontium such as collagens. As a consequence, it is possible that collagen-binding cells may change their phenotypic traits. Though the glucose derived product methylglyoxal has been detected in periodontal lesions, the precise effect of collagen glycation on gingival connective tissue biology is far from being understood. In the present study we have evaluated whether collagen glycation by methylglyoxal may affect the phenotypic properties and remodeling capacity of human gingival fibroblasts...
May 18, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28513634/mitochondria-targeted-platinum-ii-complexes-dual-inhibitory-activities-on-tumor-cell-proliferation-and-migration-invasion-via-intracellular-trafficking-of-%C3%AE-catenin
#19
Jingling Li, Xiaoli He, Yeling Zou, Dandan Chen, Liecheng Yang, Jiaming Rao, Huabing Chen, Michael C W Chan, Lin Li, Zhengqing Guo, Leshuai W Zhang, Chunying Chen
Mitochondria-targeted therapy is an alternative strategy for cancer therapy and may overcome the problems of metastasis and drug resistance that usually occur in conventional treatment. In this work, we demonstrate the mitochondria-targeted delivery of a cationic cyclometalated platinum(ii) complex, PIP-platin, in cancer cells. PIP-platin showed selective delivery and accumulation in the mitochondria and exhibited toxicity against a variety of tumor cell lines. The mitochondria were disrupted by PIP-platin, along with the generation of reactive oxygen species, depolarization of mitochondrial membrane potential, release of cytochrome c and necrosis...
May 17, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/28512053/effect-of-chitosan-and-coagulation-factors-on-the-wound-repair-phenotype-of-bioengineered-blood-clots
#20
Caroline D Hoemann, Catherine Marchand, Georges-Etienne Rivard, Hani El-Gabalawy, Patrice E Poubelle
Controlling the blood clot phenotype in a surgically prepared wound is an evolving concept in scaffold-guided tissue engineering. Here, we investigated the effect of added chitosan (80% or 95% Degree of Deacetylation, DDA) or coagulation factors (recombinant human Factor VIIa, Tissue Factor, thrombin) on inflammatory factors released by blood clots. We tested the hypothesis that 80% DDA chitosan specifically enhances leukotriene B4 (LTB4) production. Human or rabbit whole blood was combined with isotonic chitosan solutions, coagulation factors, or lipopolysaccharide, cultured in vitro at 37°C, and after 4hours the serum was assayed for LTB4 or inflammatory factors...
May 13, 2017: International Journal of Biological Macromolecules
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