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https://www.readbyqxmd.com/read/28938611/dickkopf-1-induces-angiogenesis-via-vegf-receptor-2-regulation-independent-of-the-wnt-signaling-pathway
#1
Sung Hoon Choi, Hyemi Kim, Hyun Gyu Lee, Beom Kyung Kim, Jun Yong Park, Do Young Kim, Sang Hoon Ahn, Kwang-Hyub Han, Seung Up Kim
Tumor angiogenesis is essential for invasive tumor growth and metastasis. Dickkopf-1 (DKK-1), an antagonist of Wnt signaling, participates in tumor development and progression. We evaluated whether DKK-1 stimulation induces angiogenesis and the endothelial-mesenchymal transition (EnMT). Human umbilical vein endothelial cells (HUVECs) were stimulated with recombinant DKK-1 (rDDK-1) or conditioned medium from a culture of DKK-1-transfected 293 cells. Following stimulation, the expression levels of angiogenesis-related factors and EnMT related markers were determined by immunoblot assays...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28937529/effects-of-propofol-on-wound-closure-and-barrier-function-of-cultured-endothelial-cells-an-in-vitro-experimental-study
#2
Karina Zitta, Lars Hummitzsch, Ole Broch, Matthias Gruenewald, Henning Ohnesorge, Kerstin Parczany, Markus Steinfath, Martin Albrecht
BACKGROUND: Propofol is widely used in routine clinical practice for the induction and maintenance of anaesthesia. Although propofol is regarded as a well tolerated anaesthetic, its effect on intact or damaged endothelial cells has not yet been elucidated. OBJECTIVE: The aim of this study was to investigate the effects of different concentrations of propofol on cell damage, metabolic activity, barrier function and wound healing capacity of human endothelial cells...
September 20, 2017: European Journal of Anaesthesiology
https://www.readbyqxmd.com/read/28937457/is-a-drain-tip-culture-required-after-spinal-surgery
#3
Kazuyoshi Kobayashi, Shiro Imagama, Zenya Ito, Kei Ando, Hideki Yagi, Tetsuro Hida, Kenyu Ito, Yoshimoto Ishikawa, Mikito Tsushima, Naoki Ishiguro
STUDY DESIGN: The efficacy of use of a drain tip culture for early detection of surgical-site infection (SSI) was investigated in 329 patients after spinal surgery. OBJECTIVE: To examine the efficacy of a wound drain tip culture for detection of SSI in spinal surgery. SUMMARY OF BACKGROUND DATA: A complication of SSI after spinal surgery has high associated morbidity and mortality, and is often difficult to treat. MATERIALS AND METHODS: The subjects were patients who underwent spinal surgery at our institution between January 2010 and March 2013...
October 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28928851/%C3%AE-transducin-repeat-containing-e3-ubiquitin-protein-ligase-inhibits-migration-invasion-and-proliferation-of-glioma-cells
#4
Jun Liang, Wei-Feng Wang, Shao Xie, Xian-Li Zhang, Wei-Feng Qi, Xiu-Ping Zhou, Jin-Xia Hu, Qiong Shi, Ru-Tong Yu
β-transducin repeat-containing E3 ubiquitin protein ligase (β-TrCP) serves as the substrate recognition subunit for the Skp1-Cullin1-F-box protein E3 ubiquitin ligase, which recognizes the double phosphorylated DSG (X)2+nS destruction motif in various substrates that are essential for numerous aspects of tumorigenesis and regulates several important signaling pathways. However, the biological significance of β-TrCP in glioma progression remains largely unknown. A previous study by the authors demonstrated that the levels of β-TrCP protein expression in brain glioma tissues were significantly lower compared with non-tumorous tissues and that higher grades of gliomas exhibited lower levels of β-TrCP expression in comparison with lower glioma grades...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28926194/inverse-response-of-osteoblasts-and-fibroblasts-to-growth-on-carbon-deposited-titanium-surfaces
#5
Chie Yoshihara, Takeshi Ueno, Peng Chen, Yusuke Tsutsumi, Takao Hanawa, Noriyuki Wakabayashi
Titanium implant surfaces may serve as attachment substrates for various cell types. Since carbon adsorption on titanium is inevitable, this study examined the negative/positive biological reaction of osteoblasts and fibroblasts on carbon-deposited titanium surfaces. Osteogenic MC3T3-E1 and fibrogenic NIH/3T3 cells were separately cultured on titanium disks on which carbon deposition was experimentally regulated to achieve titanium/carbon ratios of 6.5, 0.02, 0.005, and 0. The initial attachment of cells demonstrated that the quantity of attached osteoblasts on Ti/C (0...
September 19, 2017: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/28923241/opposing-roles-of-epidermal-integrins-%C3%AE-3%C3%AE-1-and-%C3%AE-9%C3%AE-1-in-regulation-of-mtld-bmp-1-mediated-laminin-%C3%AE-2-processing-during-wound-healing
#6
Whitney M Longmate, Scott P Lyons, Lori DeFreest, Livingston Van De Water, C Michael DiPersio
Proteolytic processing of the laminin-γ2 chain is a hallmark of basement membrane maturation in the skin. Integrin α3β1, a major receptor for epidermal adhesion to laminin-332, is critical for proper basement membrane organization during skin development and wound healing. Previously, we identified a role for α3β1 in promoting the processing of laminin-γ2 in cultured keratinocytes in vitro and in wound epidermis in vivo. In the current study we identify the Bmp1 gene, which encodes variants of the mammalian tolloid/bone morphogenetic protein-1 metalloproteases, as a critical regulator of α3β1-dependent laminin-γ2 processing, thereby expanding the role of this integrin in controlling the secretion by the epidermis of factors that modulate the tissue microenvironment...
September 15, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28922518/accelerated-epithelialization-and-improved-wound-healing-metrics-in-porcine-full-thickness-wounds-transplanted-with-full-thickness-skin-micrografts
#7
Christina L Rettinger, John L Fletcher, Anders H Carlsson, Rodney K Chan
Split-thickness skin grafting (STSG) is the current gold standard for treatment of extensive burn and traumatic skin injuries. However, STSG is limited by donor-site morbidity and availability, and often leads to scarring and wound contracture. Furthermore, these thin grafts lack dermal elements such as nerves and adnexa which are important in recapitulating normal skin function. Methods of fractional skin replacement either as minced STSGs or microscopic skin tissue columns (MSTCs) have been proposed, though these techniques have not been fully-characterized and lack evidence of regenerated adnexal structures...
September 18, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28920097/acute-cd47-blockade-during-ischemic-myocardial-reperfusion-enhances-phagocytosis-associated-cardiac-repair
#8
Shuang Zhang, Xin-Yi Yeap, Matthew DeBerge, Nivedita K Naresh, Kevin Wang, Zhengxin Jiang, Jane E Wilcox, Steven M White, John P Morrow, Paul W Burridge, Daniel Procissi, Evan A Scott, William Frazier, Edward B Thorp
Our data suggest that, after a myocardial infarction, integrin-associated protein CD47 on cardiac myocytes is elevated. In culture, increased CD47 on the surface of dying cardiomyocytes impairs phagocytic removal by immune cell macrophages. After myocardial ischemia and reperfusion, acute CD47 inhibition with blocking antibodies enhanced dead myocyte clearance by cardiac phagocytes and also improved the resolution of cardiac inflammation, reduced infarct size, and preserved cardiac contractile function. Early targeting of CD47 in the myocardium after reperfusion may be a new strategy to enhance wound repair in the ischemic heart...
August 2017: JACC. Basic to Translational Science
https://www.readbyqxmd.com/read/28918534/do-surgical-helmet-systems-affect-intraoperative-wound-contamination-a-randomised-controlled-trial
#9
Otis C Shirley, Ali Bayan, Mark Zhu, James P Dalton, Siouxsie Wiles, Simon W Young
BACKGROUND: Deep infection following arthroplasty remains a devastating complication. Some registry data suggests that modern positive-pressure surgical helmet systems (SHS) are associated with a paradoxical increase in infection rates, and as such their role in arthroplasty remains unclear. The aim of this study was to investigate whether SHS increase wound contamination in total knee arthroplasty (TKA) and if this contamination can be reduced by placing tape around the gown/glove interface...
September 16, 2017: Archives of Orthopaedic and Trauma Surgery
https://www.readbyqxmd.com/read/28918465/congenital-lumbar-kyphosis-with-skin-ulceration-and-osteomyelitis-in-a-myelomeningocele-child-a-case-report
#10
Benoit Vibert, Marco Turati, Pierre-Yves Rabattu, Marco Bigoni, Ahmad Eid, Aurélien Courvoisier
INTRODUCTION: Kyphosis is a frequent problem in children with spina bifida, and this deformity may cause different complications as respiratory insufficiency, bowel dysfunction, and skin ulcers. CASE REPORT: We report on a 13-year-old myelomeningocele male with a lumbar kyphoscoliosis associated to a septic skin ulceration that resulted in an acute sepsis. An X-ray revealed a kyphosis of 110° and a scoliosis of 25° between T9 and L5. The wound and blood cultures showed Staphylococcus aureus colonization, and an appropriate antibiotic therapy was started...
September 16, 2017: Child's Nervous System: ChNS: Official Journal of the International Society for Pediatric Neurosurgery
https://www.readbyqxmd.com/read/28916996/successive-release-of-tissue-inhibitors-of-metalloproteinase-1-through-graphene-oxide-based-delivery-system-can-promote-skin-regeneration
#11
Cheng Zhong, Dike Shi, Yixiong Zheng, Peter J Nelson, Qi Bao
The purpose of this study was to testify the hypothesis that graphene oxide (GO) could act as an appropriate vehicle for the release of tissue inhibitors of metalloproteinase-1 (TIMP-1) protein in the context of skin repair. GO characteristics were observed by scanning electron microscopy, atomic force microscopy, and thermal gravimetric analysis. After TIMP-1 absorbing GO, the release profiles of various concentrations of TIMP-1 from GO were compared. GO biocompatibility with fibroblast viability was assessed by measuring cell cycle and apoptosis...
September 15, 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28916796/low-temperature-plasma-promoting-fibroblast-proliferation-by-activating-the-nf-%C3%AE%C2%BAb-pathway-and-increasing-cyclind1-expression
#12
Jin-Ren Liu, Gui-Min Xu, Xing-Min Shi, Guan-Jun Zhang
The potential applications of low temperature plasma (LTP) in wound healing have aroused the concern of many researchers. In this study, an argon atmospheric pressure plasma jet was applied to generate LTP for treatment of murine fibroblast cell (L929) cultured in vitro to investigate the effect of NF-κB pathway on fibroblast proliferation. The results showed that, compared with the control, L929 cells treated with plasma for less than 20 s had significant increases of proliferation; the productions of intracellular ROS, O2(-) and NO increased with prolongation of LTP treatment time; NF-κB pathway was activated by LTP in a proper dose range, and the expression of cyclinD1 in LTP-treated cells increased with the same trend as cell proliferation...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28916766/stimulatory-secretions-of-airway-epithelial-cells-accelerate-early-repair-of-tracheal-epithelium
#13
Egi Kardia, Rafeezul Mohamed, Badrul Hisham Yahaya
Airway stem/progenitor epithelial cells (AECs) are notable for their differentiation capacities in response to lung injury. Our previous finding highlighted the regenerative capacity of AECs following transplantation in repairing tracheal injury and reducing the severity of alveolar damage associated acute lung injury in a rabbit model. The goal of this study is to further investigate the potential of AECs to re-populate the tracheal epithelium and to study their stimulatory effect on inhibiting pro-inflammatory cytokines, epithelial cell migration and proliferation, and epithelial-to-mesenchymal transition (EMT) process following tracheal injury...
September 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28913290/delayed-onset-methicillin-resistant-staphylococcus-aureus-infection-at-18-months-after-absorbable-plate-fixation-for-zygomaticomaxillary-complex-fracture
#14
Hyun Rok Lee, Hea Kyeong Shin, Dong Lark Lee, Gyu Yong Jung
None of the reports of delayed infection mentioned a latent period exceeding 13 months. we report an infection that developed 18 months after implantation of an absorbable plate. A 16-year-old adolescent girl had undergone reduction and fixation with an absorbable plate for Lefort I and zygomaticomaxillary complex fractures 18 months prior at our hospital. In her most recent hospital visit as an outpatient, abscess was observed in periocular area. Computed tomography revealed sinusitis with an abscess above the infraorbital rim...
December 2016: Arch Craniofac Surg
https://www.readbyqxmd.com/read/28902422/transplantation-of-non-cultured-stromal-vascular-fraction-cells-of-adipose-tissue-ameliorates-osteonecrosis-of-the-jaw-like-lesions-in-mice
#15
Shinichiro Kuroshima, Muneteru Sasaki, Kazunori Nakajima, Saki Tamaki, Hiroki Hayano, Takashi Sawase
The precise pathoetiology and effective treatment strategies for bisphosphonate-related osteonecrosis of the jaw (BRONJ) remain unknown. Transplantation of non-cultured stromal vascular fraction (SVF) cells has been demonstrated to be a useful method for regenerative medicine in place of stem cell therapy. This study investigated the effects of non-cultured SVF transplantation on tooth extraction socket healing in mice. Both chemotherapeutic/bisphosphonate combination therapy for 7 weeks and tooth extraction of maxillary first molars at 3 weeks after drug administration were performed using female C57BL/6J mice...
September 13, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/28902365/appl1-promotes-the-migration-of-gastric-cancer-cells-by-regulating-akt2-phosphorylation
#16
Yingxun Liu, Chunli Zhang, Lingyu Zhao, Ning Du, Ni Hou, Tusheng Song, Chen Huang
As a multifunctional adaptor protein, APPL1 (adaptor protein containing pleckstrin homology domain, phosphotyrosine binding domain and a leucine zipper motif 1) is overexpressed in many cancers, and has been implicated in tumorigenesis and tumor progression. The present study investigated the expression of APPL1 in gastric carcinoma and the function in regulating cell migration. We investigated the expression of APPL1 in gastric carcinoma based upon The Cancer Genome Atlas (TCGA) database. The expression of APPL1 in collected gastric carcinoma tissues and cultured cells was measured by qRT-PCR and western blot analysis...
September 5, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28902290/effect-of-secondary-infection-on-epithelialisation-and-total-healing-of-cutaneous-leishmaniasis-lesions
#17
Liliane de Fátima Antonio, Marcelo Rosandiski Lyra, Maurício Naoto Saheki, Armando de Oliveira Schubach, Luciana de Freitas Campos Miranda, Maria de Fátima Madeira, Maria Cristina da Silva Lourenço, Aline Fagundes, Érica Aparecida Dos Santos Ribeiro, Leonardo Barreto, Maria Inês Fernandes Pimentel
BACKGROUND: Cutaneous leishmaniasis (CL) generally presents with a single or several localised cutaneous ulcers without involvement of mucous membranes. Ulcerated lesions are susceptible to secondary contamination that may slow the healing process. OBJECTIVE: This study verified the influence of non-parasitic wound infection on wound closure (epithelialisation) and total healing. METHODS: Twenty-five patients with a confirmed diagnosis of CL and ulcerated lesions underwent biopsy of ulcer borders...
September 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28902018/mechanism-of-proliferation-of-cultured-human-corneal-endothelial-cells
#18
Takeshi Joko, Atsushi Shiraishi, Takeshi Kobayashi, Yuichi Ohashi, Shigeki Higashiyama
Because human corneal endothelial cells (HCECs) do not proliferate once the endothelial monolayer has formed, corneal wound healing is believed to be mediated by cell enlargement or migration, rather than by proliferation. However, the cellular mechanisms involved in wound healing by HCECs have not been fully determined. In this review, we focus on the effects of promyelocytic leukemia zinc finger (PLZF), a DNA-binding transcription factor, and transforming growth factor (TGF)-β2 on the proliferation and migration of cultured HCECs...
September 7, 2017: Cornea
https://www.readbyqxmd.com/read/28901412/differential-mirna-expression-profiles-in-human-keratinocytes-in-response-to-protein-kinase-c-inhibitor
#19
Yang Liu, Liangchen Zhong, Dewu Liu, Hua Ye, Yuangui Mao, Yanghong Hu
Aberrant expression of microRNAs (miRNAs) is widely accepted to be involved in keratinocyte differentiation and to be dependent on activation of the protein kinase C (PKC) pathway. However, the miRNA profiles and biological characteristics of keratinocytes induced by specific inhibitors of PKC have yet to be elucidated. The present study aimed to explore the differential miRNA expression profiles in keratinocytes treated with the PKC inhibitor GF109203X, by conducting a bioinformatics analysis. Parts of the GF109203X‑induced keratinocytes formed distinct clones after 2 days of culture, and the expression of intergrin β1, cytokeratin (CK)19 and CK14 were positive, whereas CK10 expression was negative...
September 8, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28899582/fgf2-mediated-attenuation-of-myofibroblast-activation-is-modulated-by-distinct-mapk-signaling-pathways-in-human-dermal-fibroblasts
#20
David M Dolivo, Sara A Larson, Tanja Dominko
BACKGROUND: Previous human and animal studies have demonstrated the ability of exogenously administered basic fibroblast growth factor (FGF2) to act as an antifibrotic agent in the skin. Though the activity of FGF2 as an anti-scarring agent is well-established for fibrotic skin wounds, the mechanisms by which FGF2 exerts these actions are not entirely understood. Canonical FGF2 signaling proceeds in part via FGFR/MAPK pathways in human dermal fibroblasts, and FGF2 has been described to prevent or reverse the fibroblast-to-myofibroblast transition, which is driven by TGFβ signaling and understood to be an important step in the formation of a fibrotic scar in vivo...
September 1, 2017: Journal of Dermatological Science
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