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https://www.readbyqxmd.com/read/29331905/macrophage-phenotype-switch-by-sequential-action-of-immunomodulatory-cytokines-from-hydrogel-layers-on-titania-nanotubes
#1
Junhong Chen, Mengting Li, Congling Yang, Xianzhen Yin, Ke Duan, Jianxin Wang, Bo Feng
Inflammatory response occurring between tissues and implants after implantation has attracted increasing attention because it can cause local tissue necrosis and even implant failure. Macrophages play a key role in all stages of inflammation. Pro-inflammatory (M1) and anti-inflammatory (M2) macrophages comprise two main phenotypes and the switch from M1 to M2 at specific time points is important for wound healing and tissue regeneration. Therefore, we hypothesized that biomaterial systems capable of facilitating macrophage phenotype switching should attenuate inflammation and enhance healing...
January 9, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29331783/comparative-study-of-novel-in-situ-decorated-porous-chitosan-selenium-scaffolds-and-porous-chitosan-silver-scaffolds-towards-antimicrobial-wound-dressing-application
#2
Dhee P Biswas, Neil M O'Brien-Simpson, Eric C Reynolds, Andrea J O'Connor, Phong A Tran
Dermal defects caused by trauma or disease are challenging to treat due to difficult-to-treat infections that impair wound healing. Due to the widespread emergence of drug-resistant bacteria and dwindling discoveries of new antibiotics, there is currently an urgent need to introduce novel antimicrobials effective against antibiotic-resistant bacteria without causing damage to host tissues. As selenium (Se) and silver (Ag) are known for their antimicrobial properties, we investigated the separate loading of these materials into porous chitosan/PVA (CS) scaffolds through a simple in situ deposition method to create two distinct wound dressing materials (CS-Se and CS-Ag)...
January 5, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29331377/%C3%AE-cellulin-promotes-the-proliferation-of-corneal-epithelial-stem-cells-through-the-phosphorylation-of-erk1-2
#3
Won-Yong Jeong, Hye-Young Yoo, Chan-Wha Kim
The proliferation of corneal epithelial stem cells (CESCs) is a very important process in the recovery of corneal wounds. Recent studies have shown that β-cellulin (BC) is effective in the repair of other tissues. However, its mechanism of action in corneal wound healing is not yet clear. The purpose of this study was to investigate how BC accelerates wound healing of the cornea. Here, we confirmed that the proliferation of CESCs was induced at a specific concentration (0.2, 2 and 20 ng/mL) by treatment with BC...
January 10, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29330848/cultured-allogeneic-fibroblast-injection-versus-fibroblasts-cultured-on-amniotic-membrane-scaffold-for-dystrophic-epidermolysis-bullosa-treatment
#4
H Moravvej, F Abdollahimajd, M-H Naseh, Z Piravar, E Abolhasani, N Mozafari, H Niknejad
BACKGROUND: Different methods of fibroblast application have been examined to treat recessive dystrophic epidermolysis bullosa (RDEB). OBJECTIVE: To compare the effects of intradermal injection of cultured allogeneic fibroblasts in healing RDEB wounds with that of fibroblasts seeded on amniotic membrane scaffolds (FAMS) or standard wound care (SWC) with Vaseline gauze as controls. MATERIALS &METHODS: Seven patients were recruited, and seven wounds were assessed in each patient: three wounds were treated with injection of intradermal fibroblasts, three were treated with FAMS, and one was dressed with SWC...
January 12, 2018: British Journal of Dermatology
https://www.readbyqxmd.com/read/29330743/acute-downregulation-of-mir-155-leads-to-a-reduced-collagen-synthesis-through-attenuating-macrophages-inflammatory-factor-secretion-by-targeting-ship1
#5
Longlong Yang, Lingying Liu, Huinan Ying, Yonghui Yu, Donghai Zhang, Huping Deng, Haijun Zhang, Jiake Chai
Fibrosis, tightly associated with fibroblasts collagen synthesis, is related closely with inflammatory response. Our previously study found that acute downregulation of miR-155 at wound sites leads to a reduced fibrosis, however its particular mechanism is unclear. Herein, we aimed to explore the mechanism of miR-155 in reducing fibrosis. We first found that down-regulation of miR-155 inhibited macrophages transforming growth factor-β1 (TGF-β1) and IL-1β secretion. Next, we found that co-cultured with macrophages increased the proliferation and collagen synthesis of fibroblasts, and downregulation of miR-155 in macrophages could effectively attenuate the accelerative effects...
January 12, 2018: Journal of Molecular Histology
https://www.readbyqxmd.com/read/29328541/gm-csf-ameliorates-micro-vascular-barrier-integrity-via-pericytes-derived-ang-1-in-wound-healing
#6
Min Yan, Yange Hu, Min Yao, Shisan Bao, Yong Fang
BACKGROUND: Skin wound healing involves complex coordinated interactions of cells, tissues and mediators. Maintaining microvascular barrier integrity is one of the key events for endothelial homeostasis during wound healing. Vasodilation is observed after vasoconstriction, which causes blood vessels to become porous, facilitates leukocyte infiltration and aids angiogenesis at the wound-area, post-injury. Eventually, vessel integrity has to be re-established for vascular maturation. Numerous studies have found that granulocyte macrophage colony-stimulating factor (GM-CSF) accelerates wound healing by inducing recruitment of repair cells into the injury area and releases of cytokines...
January 12, 2018: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29326040/downregulation-of-exocyst-sec10-accelerates-kidney-tubule-cell-recovery-through-enhanced-cell-migration
#7
Mi Ra Noh, Hee-Seong Jang, Dae-Kyu Song, Seong-Ryong Lee, Joshua H Lipschutz, Kwon Moo Park, Jee In Kim
Migration of surviving kidney tubule cells after sub-lethal injury, for example ischemia/reperfusion (I/R), plays a critical role in recovery. Exocytosis is known to be involved in cell migration, and a key component in exocytosis is the highly-conserved eight-protein exocyst complex. We investigated the expression of a central exocyst complex member, Sec10, in kidneys following I/R injury, as well as the role of Sec10 in wound healing following scratch injury of cultured Madin-Darby canine kidney (MDCK) cells...
January 8, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29323190/angiopoietins-bind-thrombomodulin-and-inhibit-its-function-as-a-thrombin-cofactor
#8
Christopher Daly, Xiaozhong Qian, Carla Castanaro, Elizabeth Pasnikowski, Xiabo Jiang, Benjamin R Thomson, Susan E Quaggin, Nicholas Papadopoulos, Yang Wei, John S Rudge, Gavin Thurston, George D Yancopoulos, Samuel Davis
Angiopoietin-1 (Ang1) and Angiopoietin-2 (Ang2) are ligands for Tie2, an endothelial-specific receptor tyrosine kinase that is an essential regulator of angiogenesis. Here we report the identification, via expression cloning, of thrombomodulin (TM) as another receptor for Ang1 and Ang2. Thrombomodulin is an endothelial cell surface molecule that plays an essential role as a coagulation inhibitor via its function as a cofactor in the thrombin-mediated activation of protein C, an anticoagulant protein, as well as thrombin-activatable fibrinolysis inhibitor (TAFI)...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321021/silver-pig-skin-nanocomposites-and-mesenchymal-stem-cells-suitable-antibiofilm-cellular-dressings-for-wound-healing
#9
Mario Alberto Pérez-Díaz, Phaedra Silva-Bermudez, Binisa Jiménez-López, Valentín Martínez-López, Yaaziel Melgarejo-Ramírez, Ana Brena-Molina, Clemente Ibarra, Isabel Baeza, M Esther Martínez-Pardo, M Lourdes Reyes-Frías, Erik Márquez-Gutiérrez, Cristina Velasquillo, Gabriel Martínez-Castañon, Fidel Martinez-Gutierrez, Roberto Sánchez-Sánchez
BACKGROUND: Treatment of severe or chronic skin wounds is an important challenge facing medicine and a significant health care burden. Proper wound healing is often affected by bacterial infection; where biofilm formation is one of the main risks and particularly problematic because it confers protection to microorganisms against antibiotics. One avenue to prevent bacterial colonization of wounds is the use of silver nanoparticles (AgNPs); which have proved to be effective against non-multidrug-resistant and multidrug-resistant bacteria...
January 10, 2018: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/29320769/electronic-nose-in-the-detection-of-wound-infection-bacteria-from-bacterial-cultures-a-proof-of-principle-study
#10
Taavi Saviauk, Juha P Kiiski, Maarit K Nieminen, Nelly N Tamminen, Antti N Roine, Pekka S Kumpulainen, Lauri J Hokkinen, Markus T Karjalainen, Risto E Vuento, Janne J Aittoniemi, Terho J Lehtimäki, Niku K Oksala
BACKGROUND: Soft tissue infections, including postoperative wound infections, result in a significant burden for modern society. Rapid diagnosis of wound infections is based on bacterial stains, cultures, and polymerase chain reaction assays, and the results are available earliest after several hours, but more often not until days after. Therefore, antibiotic treatment is often administered empirically without a specific diagnosis. METHODS: We employed our electronic nose (eNose) system for this proof-of-concept study, aiming to differentiate the most relevant bacteria causing wound infections utilizing a set of clinical bacterial cultures on identical blood culture dishes, and established bacterial lines from the gaseous headspace...
January 10, 2018: European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
https://www.readbyqxmd.com/read/29318413/evaluation-of-postoperative-fever-after-surgical-correction-of-neuromuscular-scoliosis-implication-on-management
#11
Mohamed Abdelhamid Ali Yousef, Scott Rosenfeld
BACKGROUND: Scoliosis is a common deformity in patients with neuromuscular disorders which usually necessitates surgical correction. Patients with neuromuscular scoliosis are characterized by increased incidence of associated medical co-morbidities and higher postoperative complication rate; therefore, these patients are often managed with a wide multidisciplinary care team. Postoperative fever is a frequent complication after surgery which is often routinely investigated using different workup tests to rule out infection...
January 9, 2018: European Spine Journal
https://www.readbyqxmd.com/read/29316701/the-effect-of-cryopreserved-human-placental-tissues-on-biofilm-formation-of-wound-associated-pathogens
#12
Yong Mao, Anya Singh-Varma, Tyler Hoffman, Sandeep Dhall, Alla Danilkovitch, Joachim Kohn
Biofilm, a community of bacteria, is tolerant to antimicrobial agents and ubiquitous in chronic wounds. In a chronic DFU (Diabetic Foot Ulcers) clinical trial, the use of a human cryopreserved viable amniotic membrane (CVAM) resulted in a high rate of wound closure and reduction of wound-related infections. Our previous study demonstrated that CVAM possesses intrinsic antimicrobial activity against a spectrum of wound-associated bacteria under planktonic culture conditions. In this study, we evaluated the effect of CVAM and cryopreserved viable umbilical tissue (CVUT) on biofilm formation of S...
January 8, 2018: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/29316016/non-invasive-measurement-of-reepithelialization-and-microvascularity-of-suction-blister-wounds-with-benchmarking-to-histology
#13
Heidi Fhaer Larsen, Malin Glindvad Ahlström, Lise Mette Rahbek Gjerdrum, Mette Mogensen, Khaled Ghathian, Henrik Calum, Anne Lyngholm Sørensen, Julie Lyng Forman, Mark Vandeven, Marian N Holerca, Laurence Du-Thumm, Lars Nannestad Jorgensen, Magnus S Ågren
We explored use of the suction-blister wound model in the assessment of not only epidermal regeneration but also pain, the microvascular response and bacteriology. The effects of topical zinc sulfate were studied to articulate the methodologies in this double-blind trial. One epidermal suction blister (10 mm) was induced on each buttock in 30 healthy volunteers (15 females:15 males) and de-roofed on day 0. The wounds were randomized to daily treatment with 1.4% zinc sulfate shower gel (n = 20), placebo (n = 20) or control (n = 20)...
January 8, 2018: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/29310381/primary-cutaneous-aspergillosis-caused-by-aspergillus-fumigatus-in-an-immunocompetent-patient-a-case-report
#14
Xiaoyan Liu, Jun Yang, Weiyuan Ma
RATIONALE: Primary cutaneous aspergillosis in immunocompromised patients has been well described in extensive investigations. However, in immunocompetent hosts, primary cutaneous infection of aspergillus occurs rarely, and remains poorly characterized. PATIENT CONCERNS: We present a case of primary cutaneous aspergillosis manifested by erythematous plague covered with flava eschar. DIAGNOSES: The patient was diagnosed with primary cutaneous aspergillosis...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29306996/yap-promotes-migration-and-invasion-of-human-glioma-cells
#15
Yu Zhang, Peng Xie, Xu Wang, Peng Pan, Yan Wang, Hao Zhang, Yu Dong, Yi Shi, Yang Jiang, Rutong Yu, Xiuping Zhou
Previously, we have reported that Yes-associated protein (YAP) is upregulated in human glioma tissues and its level is positively correlated with patient prognosis. However, the role and mechanism of YAP in the highly invasive nature of human gliomas were largely unknown. In this study, examined by wound healing assay, transwell assay, or live-imaging, we found that YAP downregulation inhibited glioma cell migration and invasion, while YAP over-expression promoted them. Interestingly, the above effect of YAP on immortalized glioma cells was recapitulated in cultured primary glioma cells...
January 6, 2018: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/29306852/trichosporon-asahii-septic-thrombophlebitis-following-lower-extremity-amputation-in-an-immunocompetent-host
#16
Pradeep Kumar Mada, Folusakin Ayoade, Ailing Li, John Todd
A 59-year-old man with a history of peripheral vascular disease status post femoral popliteal bypass presented with critical limb ischaemia of the left leg. An arterial Doppler ultrasound showed an occluded graft requiring an above knee amputation. Five days after surgery, the patient developed fever, leucocytosis, significant stump swelling and pain, and serosanguinous discharge from his wound. Wound swab cultures from the stump grew Trichosporon asahii A venous Doppler ultrasound revealed extensive thrombosis of the left lower extremity...
January 6, 2018: BMJ Case Reports
https://www.readbyqxmd.com/read/29306209/retinoid-interferon-induced-mortality19-grim19-inhibits-proliferation-and-invasion-in-rheumatoid-arthritis-fibroblast-like-synoviocytes
#17
Dongkai Zhao, Zhiyu Jiang, Zhihui Wang, Jinliang Gao
Rheumatoid arthritis (RA) fibroblast-like synoviocytes (RA-FLS) are reportedly involved in RA initiation, progression, and perpetuation. Previously a study showed that retinoid interferon-induced mortality 19 (GRIM19) improved the clinical and histological features of collagen-induced arthritis (CIA),and also inhibited osteoclast formation. However, the role of GRIM19 in RA-FLS remains unclear. In this study, we explored the biological function and underlying mechanism of GRIM19 in cultured RA-FLS. The expression of GRIM19 in synovial tissues and RA-FLS was detected by real-time quantitative RT-PCR(qRT-PCR)...
January 3, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29305678/the-role-of-adipose-derived-stem-cells-in-a-self-organizing-3d-model-with-regard-to-human-soft-tissue-healing
#18
Martin Oberringer, Monika Bubel, Martina Jennewein, Silke Guthörl, Tamara Morsch, Sophie Bachmann, Wolfgang Metzger, Tim Pohlemann
The clinical phenomenon of inadequate soft tissue healing still remains an important issue. The occurrence of chronic wounds is correlated to the life span, which is still increasing in western countries. Tissue engineering products containing adipose-derived stem cells are discussed as a promising therapeutic approach. Several studies confirmed the value of these cells for soft tissue healing improvement, suggesting a paracrine as well as a direct effect on vessel repair and angiogenesis. In an attempt to figure out specific effects of adipose-derived stem cells on dermal microvascular endothelial cells with respect to the different phases of soft tissue healing, we designed a 3D in vitro model on the basis of spheroids...
January 5, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29305548/epidemiology-clinical-characteristics-and-antimicrobial-susceptibility-profiles-of-human-clinical-isolates-of-staphylococcus-intermedius-group
#19
Melanie L Yarbrough, William Lainhart, C A Burnham
The veterinary pathogens in Staphylococcus intermedius group (SIG) are increasingly recognized as causes of human infection. Shared features between SIG and Staphylococcus aureus may result in misidentification of SIG in human clinical cultures. This study examined the clinical and microbiological characteristics of isolates recovered at a tertiary care academic medical center. From 2013 to 2015, 81 SIG isolates were recovered from 62 patients. Patients were commonly ≥ 50 years old, diabetic, or immunocompromised...
January 5, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29304107/allene-oxide-synthase-allene-oxide-cyclase-and-jasmonic-acid-levels-in-lotus-japonicus-nodules
#20
Anna Zdyb, Marco G Salgado, Kirill N Demchenko, Wolfram G Brenner, Małgorzata Płaszczyca, Michael Stumpe, Cornelia Herrfurth, Ivo Feussner, Katharina Pawlowski
Jasmonic acid (JA), its derivatives and its precursor cis-12-oxo phytodienoic acid (OPDA) form a group of phytohormones, the jasmonates, representing signal molecules involved in plant stress responses, in the defense against pathogens as well as in development. Elevated levels of JA have been shown to play a role in arbuscular mycorrhiza and in the induction of nitrogen-fixing root nodules. In this study, the gene families of two committed enzymes of the JA biosynthetic pathway, allene oxide synthase (AOS) and allene oxide cyclase (AOC), were characterized in the determinate nodule-forming model legume Lotus japonicus JA levels were to be analysed in the course of nodulation...
2018: PloS One
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