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https://www.readbyqxmd.com/read/29457968/in-vitro-and-in-vivo-effects-of-mk2206-and-chloroquine-combination-therapy-on-endometriosis-autophagy-may-be-required-for-regrowth-of-endometriosis
#1
Sachiko Matsuzaki, Jean-Luc Pouly, Michel Canis
BACKGROUND AND PURPOSE: A high recurrence rate after medical treatment is a major clinical problem for patients with endometriosis. In the present study, we evaluated the in vitro effects of combined treatment with MK2206 (an AKT inhibitor) + chloroquine on cell growth and regrowth of endometriotic stromal cells, and the in vivo effects on endometriotic implants in a mouse xenograft model of endometriosis. EXPERIMENTAL APPROACH: We evaluated the effects of autophagy inhibition by knockdown of the ATG13, Beclin-1, and ATG12 genes and pharmacologic agents (chloroquine, bafilomycin A1, or 3-methyalanine) individually and in combination with MK2206 on cell growth and/or cell regrowth of endometriotic stromal cells in vitro...
February 19, 2018: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29456707/resveratrol-induces-autophagic-apoptosis-via-the-lysosomal-cathepsin-d-pathway-in-human-drug-resistant-k562-adm-leukemia-cells
#2
Zhewen Zhang, Zhuan Liu, Jing Chen, Juan Yi, Juan Cheng, Wangqing Dun, Hulai Wei
The aim of the present study was to investigate the crosstalk between resveratrol (Res)-induced autophagy and apoptosis, and the molecular pathway by which autophagy leads to apoptotic death in drug-resistant K562/ADM leukemia cells. The viability of K562/ADM cells was determined using the MTT assay. The formation of autophagic vacuoles was detected using transmission electron microscopy and monodansylcadaverine (MDC) staining. Cell apoptosis was evaluated using flow cytometry. The expression of apoptosis- or autophagy-associated proteins was measured using western blotting...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29456633/crosstalk-between-apoptosis-and-autophagy-in-prostate-epithelial-cells-under-androgen-deprivation
#3
Rong-Fu Liu, Jian Li, Jie Zhang, Pei-De Bai, Yu-Feng Yang, Wei Li, Zhun Wu, Jia-Xin Zheng
The present study investigated the molecular mechanism of apoptosis and autophagy in prostate epithelial cells under androgen deprivation (AD). BPH-1 cells were divided into four groups as follows: Control (Cont), AD, autophagy inhibition (AI) and AD + AI groups. Cells in the four groups were treated accordingly, and the level of apoptosis was subsequently measured via flow cytometry. The expression of the microtubule-associated proteins 1A/1B light chain 3 (LC3), caspase-3, poly (ADP-ribose) polymerase 1 (PARP-1) and Beclin-1 proteins of BPH-1 cells was detected at different time points following culture in androgen-deprived medium...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29456493/administration-of-ketamine-causes-autophagy-and-apoptosis-in-the-rat-fetal-hippocampus-and-in-pc12-cells
#4
Xinran Li, Yanan Li, Jinghua Zhao, Lina Li, Yuxin Wang, Yiming Zhang, Yue Li, Yu Chen, Wenhan Liu, Li Gao
Drug abuse during pregnancy is a serious problem. Like alcohol, anticonvulsants, sedatives, and anesthetics, such as ketamine, can pass through the placental barrier and affect the growing fetus. However, the mechanism by which ketamine causes damage to fetal rats is not well understood. Therefore, in this study, we anesthetized pregnant rats with ketamine and evaluated the Total Antioxidant Capacity (T-AOC), Reactive Oxygen Species (ROS), and Malondialdehyde (MDA). Moreover, we determined changes in the levels of Cleaved-Caspase-3 (C-Caspase-3), Beclin-1, B-cell lymphoma-2 (Bcl-2), Bcl-2 Associated X Protein (Bax), Autophagy-related gene 4 (Atg4), Atg5, p62 (SQSTM1), and marker of autophagy Light Chain 3 (LC3)...
2018: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29452162/pseudorabies-virus-induces-autophagy-to-enhance-viral-replication-in-mouse-neuro-2a-cells-in-vitro
#5
Changmeng Xu, Mi Wang, Zhongbao Song, Zhijian Wang, Qianyu Liu, Ping Jiang, Juan Bai, Yufeng Li, Xianwei Wang
Autophagy of cytoplasmic components plays an essential role in the pathogenic infection process. Furthermore, research suggests that autophagy is an extremely important component of the innate immune response. Our study aimed to reveal the effect of virus-induced autophagy on pseudorabies virus (PRV) replication. Our results confirmed that light chain 3 (LC3)-I was converted into LC3-II after PRV infection; this transition is considered an important indicator of autophagy. Transmission electron microscopy (TEM) revealed that PRV infection could notably increase the number of autophagosomes in mouse neuro-2a (N2a) cells...
February 13, 2018: Virus Research
https://www.readbyqxmd.com/read/29436654/tetrahydrocurcumin%C3%A2-induced-autophagy-via-suppression-of-pi3k-akt-mtor-in-non%C3%A2-small-cell-lung-carcinoma-cells
#6
Guoqiang Song, Honghui Lu, Fei Chen, Youmei Wang, Weibin Fan, Weifang Shao, Huoquan Lu, Bin Lin
Lung carcinoma is the leading cause of mortality due to cancer worldwide. Autophagy has a significant role in the development and progression of non‑small cell lung carcinoma (NSCLC). A previous study has revealed that tetrahydrocurcumin (THC), a traditional Chinese medicine isolated from Curcuma wenyujin (Chen & Ling, 1981), induces autophagy in human A549 NSCLC cells. The present study evaluated THC‑induced autophagy in A549 cells using various assays, including the Cell Counting Kit‑8, acridine orange staining, flow cytometry, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), and western blot analysis of the markers of autophagy...
February 13, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29436652/neuronalinjury-and-roles-of-apoptosis-and-autophagy-in-a-neonatal-rat-model-of-hypoxia-ischemia-induced-periventricular-leukomalacia
#7
Lin Qiao, Jianhua Fu, Xindong Xue, Yongyan Shi, Li Yao, Wanjie Huang, Jun Li, Dan Zhang, Na Liu, Xin Tong, Yanna Du, Yuqing Pan
As research into periventricular leukomalacia (PVL) gradually increases, concerns are emerging about long‑term neuron injury. The present study aimed to investigate neuronal injury and the relevant alterations in apoptosis and autophagy in a PVL model established previously. A rat model of hypoxia‑ischemia‑induced PVL was established. In the model group, Sprague‑Dawley (SD) rats [postnatal day 3 (P3)] were subjected to right common carotid artery ligation followed by suturing and exposed to 6‑8% oxygen for 2 h; in the control group, SD rats (P3) were subjected to right common carotid artery dissection followed by suturing, without ligation and hypoxic exposure...
February 7, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29435149/mir-361-5p-suppresses-chemoresistance-of-gastric-cancer-cells-by-targeting-foxm1-via-the-pi3k-akt-mtor-pathway
#8
Lei Tian, Zhifeng Zhao, Ling Xie, JinPeng Zhu
Gastric cancer is a prevalent cancer and chemotherapy is a main treatment for patients. Docetaxel is commonly used as a chemotherapeutic drug for gastric cancer patients. With the increasing emergence of docetaxel resistance, exploring the mechanism of chemoresistance may improve prognosis of patients. In this study, we found that overexpressed miR-361-5p suppressed chemoresistance to docetaxel of gastric cancer cells (SGC-7901, MKN-28) by decreasing IC 50 values of docetaxel while increasing cell apoptosis rate, especially in docetaxel resistant SGC-7901 cells...
January 12, 2018: Oncotarget
https://www.readbyqxmd.com/read/29435061/beclin-1-knockdown-decreases-proliferation-invasion-and-migration-of-ewing-sarcoma-sk-es-1-cells-via-inhibition-of-mmp-9
#9
Conglin Ye, Xiaolong Yu, Xuqiang Liu, Ping Zhan, Tao Nie, Runsheng Guo, Hucheng Liu, Min Dai, Bin Zhang
Although Beclin-1, a well-known key regulator of autophagy, has been demonstrated to serve a function in a number of disorders, including cancer, aging and degenerative diseases, its biological function in Ewing sarcoma (ES) remains unresolved. The objective of the present study was to determine the in vitro effect of Beclin-1 knockdown on the growth and malignant phenotype of ES SK-ES-1 cells, which have increased endogenous expression of Beclin-1 compared with RD-ES cells, and to investigate the underlying molecular mechanism...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29435046/effect-of-silencing-colon-cancer-associated-transcript-2-on-the-proliferation-apoptosis-and-autophagy-of-gastric-cancer-bgc-823-cells
#10
Zhao-Yan Yu, Zi Wang, Ke-Yue Lee, Ping Yuan, Jie Ding
The role of long non-coding RNAs (lncRNAs) in the carcinogenesis and progression of tumors has been receiving increasing attention. Colon cancer-associated transcript 2 (CCAT2), a type of oncogenic lncRNA, is regarded as a novel biomarker of poor prognosis and metastasis in various types of cancer. However, the molecular contributions of CCAT2 to gastric cancer (GC) progression remain largely unclear. The aim of the present study was to demonstrate the effect of silencing CCAT2 on the biological behavior of GC BGC-823 cells and illustrate the potential underlying molecular mechanisms...
March 2018: Oncology Letters
https://www.readbyqxmd.com/read/29434961/effect-and-molecular-mechanism-of-mtor-inhibitor-rapamycin-on-temozolomide-induced-autophagic-death-of-u251-glioma-cells
#11
Bing Li, Chun Zhou, Liang Yi, Lunshan Xu, Minhui Xu
Glioma is a malignant tumor of the glial tissue that is difficult to excise through surgery, with poor patient prognosis. The use of chemotherapeutic drugs alone to treat glioma following surgery results in a high probability of sequelae, such as tumor recurrence. The present study investigated the effects of a novel treatment combination on glioma cells and determined the molecular mechanisms underlying its action. The effect of temozolomide (TMZ) combined with rapamycin (RAPA) on the TMZ-induced autophagic death of U251 glioma cells was examined...
February 2018: Oncology Letters
https://www.readbyqxmd.com/read/29427791/cyp2j2-derived-eets-attenuated-ethanol-induced-myocardial-dysfunction-through-inducing-autophagy-and-reducing-apoptosis
#12
Chi Zhou, Jin Huang, Qing Li, Chenao Zhan, Xizhen Xu, Xu Zhang, Ding Ai, Yi Zhu, Zheng Wen, Dao Wen Wang
Chronic excessive drinking leads to myocardial contractile dysfunction and dilated cardiomyopathy, where ethanol toxicity plays an essential role. Cytochrome P450 (CYP) epoxygenases metabolize arachidonic acids to form epoxyeicosatrienoic acids (EETs), which exert beneficial roles in the cardiovascular system, but their role in alcoholic cardiomyopathy is elusive. This study was designed to evaluate the effects and mechanisms of CYP2J2 gene delivery on ethanol-induced myocardial dysfunction with focus on autophagy and apoptosis...
February 7, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29425932/ablation-of-c-ebp-homologous-protein-attenuates-renal-fibrosis-after-ureteral-obstruction-by-reducing-autophagy-and-microtubule-disruption
#13
Mi Ra Noh, Chang-Hoon Woo, Mae-Ja Park, Jee In Kim, Kwon Moo Park
Fibrosis is an undesirable consequence of injury and a critical problem in many diseases. Recent studies have demonstrated an association of C/EBP homologous protein (CHOP) with fibrosis. We investigated the mechanism of CHOP in kidney fibrosis progression after unilateral ureteral obstruction (UUO) using Chop gene-deleted (Chop-/-) mice and their wild-type littermates (Chop+/+). UUO-induced kidney fibrosis was reduced in the Chop-/- than Chop+/+ mice. After UUO, CHOP expression was detected in the cytosol and nucleus of distal tubule cells and collecting duct cells of the kidney...
February 6, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29425466/mesenchymal-stem-cells-pretreated-with-melatonin-ameliorate-kidney-functions-in-a-rat-model-of-diabetic-nephropathy
#14
Laila A Rashed, Samah Elattar, Nashwa Eltablawy, Hend Ashour, Lamiaa Mohamed, Yasmin El-Esawy
The aim of this study was to investigate the effect of Melatonin (MT) pretreated mesenchymal stem cells (MSCs) regenerative therapy, as a new therapeutic target in modifying underlying diabetic nephropathy (DN) pathogenesis in a rat model, and its possible effect on autophagy protein Beclin-1. Forty adult male Wistar albino rats divided into four groups: control, DN, MSCs treated (MSCs) and In-vitro Melatonin (5µM for 24 hours) pre-incubated MSCs treated group (MSCs+MT). MSCs treatment significantly improved the renal functions with amelioration of the measured underlying DN pathogenesis and elevation of Beclin-1 protein levels compared to DN group...
February 9, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29423046/vitexin-induces-apoptosis-by-suppressing-autophagy-in-multi-drug-resistant-colorectal-cancer-cells
#15
Monika Bhardwaj, Hee Jun Cho, Souren Paul, Rekha Jakhar, Imran Khan, Seon-Jin Lee, Bo-Yeon Kim, Manigandan Krishnan, Tejinder Pal Khaket, Hee Gu Lee, Sun Chul Kang
Cancer treatment is limited due to the diverse multidrug resistance acquired by cancer cells and the collateral damage caused to adjacent normal cells by chemotherapy. The flavonoid compound vitexin exhibits anti-oxidative, anti-inflammatory and anti-tumor activity. This study elucidated the antitumor effects of vitexin and its underlying mechanisms in a multi-drug resistant human colon cancer cell line (HCT-116 DR ), which exhibits higher levels of multidrug-resistant protein 1 (MDR1) expression as compared with its parental cell line (HCT-116)...
January 9, 2018: Oncotarget
https://www.readbyqxmd.com/read/29422968/clinical-value-of-caga-c-met-pi3k-and-beclin-1-expressed-in-gastric-cancer-and-their-association-with-prognosis
#16
Xiaojun Huang, Chaoqun Wang, Jinmin Sun, Jun Luo, Jiangzhou You, Linchuan Liao, Mingyuan Li
Gastric cancer (GC) is the fourth most common type of malignant tumor worldwide, and causes the second highest number of cancer-associated mortalities in 2012. Gastric tumorigenesis is a multistep and multifactorial process. In the present study, tissue microarray and immunohistochemistry analysis were used to detect cytotoxin-associated gene A (CagA), c-Met, phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) and Beclin-1 expression in 121 GC tumors and 120 normal gastric tissues. The clinical relevance and prognostic implications of CagA, c-Met, PI3K and Beclin-1 expression in GC patients were analyzed...
January 2018: Oncology Letters
https://www.readbyqxmd.com/read/29422639/ileal-pouch-of-ulcerative-colitis-and-familial-adenomatous-polyposis-patients-exhibit-modulation-of-autophagy-markers
#17
Nielce Maria Paiva, Lívia Bitencourt Pascoal, Leandro Minatel Vidal Negreiros, Mariana Portovedo, Andressa Coope, Maria de Lourdes Setsuko Ayrizono, Claudio Saddy Rodrigues Coy, Marciane Milanski, Raquel Franco Leal
Total retocolectomy with ileal pouch-anal anastomosis (IPAA) is the surgery of choice for patients with ulcerative colitis (UC) that are refractory to clinical treatment. Pouchitis is one of the most common complications after this procedure. Defects in autophagy have been reported in inflammatory bowel diseases. However, there are no studies on the IP. Therefore, we studied markers for autophagy in the IP mucosa of UC and FAP patients comparing them to controls with a normal distal ileum. Sixteen patients with IP in "J" shape, asymptomatic and with endoscopically normal IP were evaluated...
February 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29416600/impaired-autophagic-flux-and-its-related-inflammation-in-patients-with-adult-onset-still-s-disease
#18
Chia-Wei Hsieh, Chun-Yu Chang, Yi-Ming Chen, Hsin-Hua Chen, Wei-Ting Hung, Ning-Rong Gung, Shiow-Jiuan Wey, Der-Yuan Chen
The pathogenic role of autophagic immune regulation in adult-onset Still's disease (AOSD) is unclear. We investigated the relative levels of autophagy in AOSD patients and healthy controls, its association with disease activity or course, and the change in autophagy after 6 months of therapy. Autophagosome levels were determined from the mean fluorescence intensity of autophagosomotropic dye incorporated into circulating immune cells. The fluorescent signal from lymphocytes, monocytes, and granulocytes from AOSD patients was greater than from controls...
January 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29412697/mir-155-affects-osteosarcoma-mg-63-cell-autophagy-induced-by-adriamycin-through-regulating-pten-pi3k-akt-mtor-signaling-pathway
#19
Lin Wang, Bing Tang, Heng Han, Dan Mao, Jie Chen, Yun Zeng, Min Xiong
OBJECTIVE: Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) plays a positive regulatory role on cell autophagy through inhibiting PI3K/AKT/mammalian target of rapamycin (mTOR) signaling pathway. miR-155 plays a critical role in osteosarcoma occurrence and chemoresistance. Bioinformatics analysis revealed the targeted binding site between miR-155 and the 3'-UTR (untranslated region) of PTEN mRNA. This study investigated the role of miR-155 in regulating osteosarcoma cell autophagy, chemosensitivity to Adriamycin (ADM), and PTEN-PI3K/AKT/mTOR signaling pathway...
February 7, 2018: Cancer Biotherapy & Radiopharmaceuticals
https://www.readbyqxmd.com/read/29408091/aspirin-induces-beclin-1-dependent-autophagy-of-human-hepatocellular-carcinoma-cell
#20
Zhenjun Huang, Weilun Fang, Weihua Liu, Li Wang, Bin Liu, Shiming Liu, Shaojun Liu
Aspirin not only reduces the incidence of hepatocellular carcinoma (HCC) but also plays a synergistic role with chemotherapy for HCC treatment. However, the underlying mechanisms remain incompletely elucidated. Given that autophagy triggers cancer cell death, the present study examined the autophagic effect of aspirin on HCC cells. Results showed that aspirin increased LC3II/LC3I ratio, decreased p62 expression, and enhanced autophagic flux (autophagosome and autolysosome puncta) in Hep3B, HepG2, or SMMC-7721 cells, reflecting the autophagy of HCC cells...
January 31, 2018: European Journal of Pharmacology
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