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https://www.readbyqxmd.com/read/28298074/evaluation-of-growth-inhibitory-response-of-resveratrol-and-salinomycin-combinations-against-triple-negative-breast-cancer-cells
#1
Girish Rai, Shankar Suman, Sanjay Mishra, Yogeshwer Shukla
Resveratrol (RSVL) a dietary phytochemical showed to enhance the efficacy of chemotherapeutic drugs. Recently, Salinomycin (SAL) has gained importance as cancer therapeutic value for breast cancer (BC), however, its superfluxious toxicity delimits the utility. Taking the advantage of RSVL, the therapeutic efficacy of RSVL and SAL combination was studied in vitro and in vivo system. Firstly, the synergistic combination dose of RSVL and SAL was calculated and further, the efficacy was examined by wound healing, and Western blots analysis...
March 11, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28295550/lncrna-malat-1-elevates-hmgb1-to-promote-autophagy-resulting-in-inhibition-of-tumor-cell-apoptosis-in-multiple-myeloma
#2
Da Gao, Zhen Xiao, Hui-Ping Li, Dong-Hai Han, Ya-Peng Zhang
BACKGROUND: Long non-coding RNAs (lncRNAs) can participate in the pathological process of multiple myeloma (MM) via regulation of specific gene expression and function. This research aimed to study the role of MALAT-1 and the underlying mechanism in MM. METHODS: In this study, the expression of MALAT-1 and HMGB1 protein in the bone marrow mononuclear cells from MM patients at different stages and in MM cell lines was determined by qRT-PCR and western blot, respectively...
March 11, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28294332/autophagy-mediates-cytotoxicity-of-human-colorectal-cancer-cells-treated-with-garcinielliptone-fc
#3
Shen-Jeu Won, Cheng-Hsin Yen, Ting-Yu Lin, Ya-Fen Jiang-Shieh, Chun-Nan Lin, Jyun-Ti Chen, Chun-Li Su
The tautomeric pair of garcinielliptone FC (GFC) is a novel tautomeric pair of polyprenyl benzophenonoid isolated from the pericarps of Garcinia subelliptica Merr. (G. subelliptica, Clusiaceae), a tree with abundant sources of polyphenols. Our previous report demonstrated that GFC induced apoptosis on various types of human cancer cell lines including chemoresistant human colorectal cancer HT-29 cells. In the present study, we observed that many autophagy-related genes in GFC-treated HT-29 cells were up- and down-regulated using a cDNA microarray containing oncogenes and kinase genes...
March 15, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28292026/catalpol-protects-glucose-deprived-rat-embryonic-cardiac-cells-by-inducing-mitophagy-and-modulating-estrogen-receptor
#4
Chao Lin, Ying Lu, Xiaojing Yan, Xiang Wu, Meiyu Kuai, Xin Sun, Qi Chen, Xueyun Kong, Zhaoguo Liu, Yuping Tang, Yi Jing, Yu Li, Qichun Zhang, Huimin Bian
Catalpol, a bioactive component from Rehmannia glutinosa (Di Huang), has been widely used to protect cardiomyocytes against myocardial ischemia. The aim of the present study was to investigate the anti-apoptotic and anti-oxidative effects of Catalpol on glucose-starved H9c2 cells for cardio-protection and to elucidate the underlying mechanisms. Here, we showed that Catalpol protected the glucose-starved H9c2 cells through reducing apoptosis and attenuating oxidative damage. Moreover, the increases of autophagic lysosomes, LC3, autophagic flux and autophagic vacuole were observed in Catalpol-treated cells using flow cytometer and fluorescence microscope...
March 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28288416/induction-of-reactive-oxygen-species-stimulated-distinctive-autophagy-by-chelerythrine-in-non-small-cell-lung-cancer-cells
#5
Zheng-Hai Tang, Wen-Xiang Cao, Zhao-Yu Wang, Jia-Hong Lu, Bo Liu, Xiuping Chen, Jin-Jian Lu
Chelerythrine (CHE), a natural benzo[c]phenanthridine alkaloid, shows anti-cancer effect through a number of mechanisms. Herein, the effect and mechanism of the CHE-induced autophagy, a type II programmed cell death, in non-small cell lung cancer (NSCLC) cells were studied for the first time. CHE induced cell viability decrease, colony formation inhibition, and apoptosis in a concentration-dependent manner in NSCLC A549 and NCI-H1299 cells. In addition, CHE triggered the expression of phosphatidylethanolamine-modified microtubule-associated protein light-chain 3 (LC3-II)...
March 9, 2017: Redox Biology
https://www.readbyqxmd.com/read/28267506/inhibition-of-acrolein-induced-autophagy-and-apoptosis-by-a-glycosaminoglycan-from-sepia-esculenta-ink-in-mouse-leydig-cells
#6
Yi-Peng Gu, Xiao-Mei Yang, Ping Luo, Yan-Qun Li, Ye-Xing Tao, Zhen-Hua Duan, Wei Xiao, Da-Yan Zhang, Hua-Zhong Liu
In our recent reports, a squid ink polysaccharide (SIP) was found having preventive activity against cyclophosphamide induced damage in mouse testis and ovary. Here we further reveal the regulative mechanism of SIP against chemical toxicity on testis. Leydig cells exposed to acrolein (ACR) underwent apoptosis at 12h and 24h. Before apoptosis, cells occurred autophagy that was confirmed by high autophagic rate and Beclin-1 protein content at 3h. PI3K/Akt and p38 MAPK signal pathways involved in the regulatory mechanisms...
May 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28266268/p2x7-receptor-orchestrates-multiple-signalling-pathways-triggering-inflammation-autophagy-and-metabolic-trophic-responses
#7
Elisa Orioli, Elena De Marchi, Anna Lisa Giuliani, Elena Adinolfi
P2X7 receptor is an ion channel activated by extracellular adenosine trisphosphate (eATP) that attracted increasing attention for its role in immune reactions, neurobiology and oncology. As receptor for an extracellular ligand, P2X7 activates a series of intracellular signalling pathways mainly via alterations of the ion permeability, but also through formation of a large unselective pore and direct interaction with other proteins. Here we wish to give an overview on the main biochemical paths initiated by P2X7 activation by revising recent and established literature on P2X7-triggered signalling cascades leading to cell death, inflammatory and immune response activation, proliferation and metabolism modulation...
March 3, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28266023/astragaloside-ii-sensitizes-human-hepatocellular-carcinoma-cells-to-5-fluorouracil-via-suppression-of-autophagy
#8
Meng Wang, Can Huang, Yong Su, Cui Yang, Quan Xia, Du-Juan Xu
OBJECTIVES: Inhibition of autophagy has been increasingly recognized as a potential therapeutic approach against cancer. Our previous reports showed that Astragaloside II improves hepatic cancer cells resistance by downregulating MDR1 and P-gp .The purpose of this study was to further investigated the effect of autophagy on AS-II reversing multidrug resistance and its molecular mechanism in hepatocellular carcinoma cells in vitro. METHODS: Bel-7402 and Bel-7402/FU cell lines were used in this study...
March 7, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28264232/memantine-induces-nmdar1-mediated-autophagic-cell-death-in-malignant-glioma-cells
#9
Wan-Soo Yoon, Mi-Young Yeom, Eun-Sun Kang, Yong-An Chung, Dong-Sup Chung, Sin-Soo Jeun
OBJECTIVE: Autophagy is one of the key responses of cells to programmed cell death. Memantine, an approved anti-dementia drug, has an antiproliferative effect on cancer cells but the mechanism is poorly understood. The aim of the present study was to test the possibility of induction of autophagic cell death by memantine in glioma cell lines. METHODS: Glioma cell lines (T-98 G and U-251 MG) were used for this study. RESULTS: The antiproliferative effect of memantine was shown on T-98 G cells, which expressed N-methyl-D-aspartate 1 receptor (NMDAR1)...
March 2017: Journal of Korean Neurosurgical Society
https://www.readbyqxmd.com/read/28260802/autophagy-inhibitor-3-methyladenine-alleviates-overload-exercise-induced-cardiac-injury-in-rats
#10
Hua Liu, Hui Lei, Yue Shi, Jin-Ju Wang, Ning Chen, Zhang-Hua Li, Yang-Fang Chen, Qi-Fa Ye, Yi Yang
Overload-exercise (OE) causes myocardial injury through inducing autophagy and apoptosis. In this study we examined whether an autophagy inhibitor 3-methyladenine (3-MA) could alleviate OE-induced cardiac injury. Rats were injected with 3-MA (15 mg/kg, iv) or saline before subjected to various intensities of OE, including no swim (control), 2 h swim (mild-intensity exercise, MIE), 2 h swim with 2.5% body weight overload (moderate OE; MOE), 5% overload (intensive OE; IOE) or 2.5% overload until exhausted (exhaustive OE; EOE)...
March 6, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28260799/bisindolylmaleimide-alkaloid-bma-155cl-induces-autophagy-and-apoptosis-in-human-hepatocarcinoma-hepg-2-cells-through-the-nf-%C3%AE%C2%BAb-p65-pathway
#11
Xiao Sun, Lin Li, Hong-Guang Ma, Pu Sun, Qi-Lin Wang, Ting-Ting Zhang, Yue-Mao Shen, Wei-Ming Zhu, Xia Li
Bisindolylmaleimides, a series of derivatives of a PKC inhibitor staurosporine, exhibit potential as anti-cancer drugs and have received considerable attention in clinical trials. This study aims to investigate the effects of a bisindolylmaleimide alkaloid 155Cl (BMA-155Cl) with a novel structure on autophagy and apoptosis in human hepatocarcinoma HepG-2 cells in vitro and in vivo. The cell poliferation was assessed with a MTT assay. Autophagy was evaluated by MDC staining and TEM analysis. Apoptosis was investigated using Annexin V-FITC/PI and DAPI staining...
March 6, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28259927/autophagy-induction-in-the-skeletal-myogenic-differentiation-of-human-tonsil-derived-mesenchymal-stem-cells
#12
Saeyoung Park, Yoonyoung Choi, Namhee Jung, Jieun Kim, Seiyoon Oh, Yeonsil Yu, Jung-Hyuck Ahn, Inho Jo, Byung-Ok Choi, Sung-Chul Jung
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation and are thus a valuable source for the replacement of diseased or damaged organs. Previously, we reported that the tonsils can be an excellent reservoir of MSCs for the regeneration of skeletal muscle (SKM) damage. However, the mechanisms involved in the differentiation from tonsil-derived MSCs (T-MSCs) to myocytes via myoblasts remain unclear. To clarify these mechanisms, we analyzed gene expression profiles of T-MSCs during differentiation into myocytes compared with human skeletal muscle cells (hSKMCs)...
February 20, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28259912/role-of-hypoxia%C3%A2-inducible-factor%C3%A2-1%C3%AE-in-autophagic-cell-death-in-microglial-cells-induced-by-hypoxia
#13
Xintao Wang, Jun Ma, Qiang Fu, Lei Zhu, Zhiling Zhang, Fan Zhang, Nan Lu, Aimin Chen
Microglial cells are phagocytic cells of the central nervous system (CNS) and have been proposed to be a primary component of the innate immune response and maintain efficient CNS homeostasis. Microglial cells are activated during various phases of tissue repair and participate in various pathological conditions in the CNS. Following spinal cord injury (SCI), anoxemia is a key problem that results in tissue destruction. Hypoxia‑inducible factor 1‑α (HIF‑1α) may protect hypoxic cells from apoptosis or necrosis under ischemic and anoxic conditions...
March 1, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28258963/interferon-%C3%AE-induced-insufficient-autophagy-contributes-to-p62-dependent-apoptosis-of-epithelial-cells-in-chronic-rhinosinusitis-with-nasal-polyps
#14
Bao-Feng Wang, Ping-Ping Cao, Zhi-Chao Wang, Zhi-Yong Li, Zhe-Zheng Wang, Jin Ma, Bo Liao, Yi-Ke Deng, Xiao-Bo Long, Kai Xu, Heng Wang, Hai Wang, Ming Zeng, Xiang Lu, Zheng Liu
BACKGROUND: Autophagy is a lysosomal degradation pathway that is essential for cell survival, differentiation, and homeostasis. This study aimed to investigate the contribution of autophagy to the pathogenesis of CRS with nasal polyps (CRSwNP). METHODS: The expression of autophagic proteins [microtubule-associated protein 1 light chain 3B (LC3B)-II, autophagy-related proteins (Atg), and Beclin 1], substrate proteins (p62 and ubiquitinated proteins), and apoptotic signaling molecules [cysteine-aspartic protease-3 and 8, and poly-ADP-ribose polymerase] in the sinonasal mucosa and nasal epithelial cells (NECs) was detected by immunohistochemistry and Western blotting...
March 4, 2017: Allergy
https://www.readbyqxmd.com/read/28254439/the-microrna-423-3p-bim-axis-promotes-cancer-progression-and-activates-oncogenic-autophagy-in-gastric-cancer
#15
Pengfei Kong, Xiaofeng Zhu, Qirong Geng, Liangping Xia, Xiaowei Sun, Yingbo Chen, Wei Li, Zhiwei Zhou, Youqing Zhan, Dazhi Xu
Human serum microRNAs (miRNAs) have been shown to serve as disease fingerprints for predicting survival of cancer patients. However, the roles of specific miRNAs involved in gastric cancer (GC) are largely unknown. In this study, miRNA profiling was performed on sera obtained from six patients in good- and poor-survival groups. Expression of miR-423-3p was validated by quantitative RT-PCR in another 67 GC serum samples and paired normal and cancerous gastric tissues. Luciferase reporter assays were used to identify the target gene Bcl-2-interacting mediator of cell death (Bim)...
February 18, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28253970/methods-to-study-the-becn1-interactome-in-the-course-of-autophagic-responses
#16
M Antonioli, F Ciccosanti, J Dengjel, G M Fimia
Autophagy is an extremely dynamic process that mediates the rapid degradation of intracellular components in response to different stress conditions. The autophagic response is executed by specific protein complexes, whose function is regulated by posttranslational modifications and interactions with positive and negative regulators. A comprehensive analysis of how autophagy complexes are temporally modified upon stress stimuli is therefore particularly relevant to understand how this pathway is regulated. Here, we describe a method to define the protein-protein interaction network of a central complex involved in autophagy induction, the Beclin 1 complex...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28253968/study-of-ulk1-catalytic-activity-and-its-regulation
#17
B Stork, J Dengjel
During the last decade, the molecular mechanisms controlling the initiation of (macro-)autophagy have been extensively studied. Two macromolecular kinase complexes are central for the initiation of autophagy: the protein kinase unc-51-like kinase 1 (ULK1) complex and the lipid kinase VPS34/Beclin 1 complex. The serine/threonine kinase ULK1 represents the mammalian ortholog of yeast autophagy-related (Atg) protein 1 (Atg1). ULK1 is regulated by upstream nutrient- and energy-sensing kinases, and transmits these signals to the core autophagic machinery...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28250335/role-of-the-pretreatment-18-f-fluorodeoxyglucose-positron-emission-tomography-maximal-standardized-uptake-value-in-predicting-outcomes-of-colon-liver-metastases-and-that-value-s-association-with-beclin-1-expression
#18
Eleonora G Dimitrova, Borislav G Chaushev, Nikolay V Conev, Javor K Kashlov, Aleksandar K Zlatarov, Dilyan P Petrov, Hristo B Popov, Nadezhda T Stefanova, Anelia D Klisarova, Kameliya Z Bratoeva, Ivan S Donev
The current study sought to evaluate the predictive and prognostic performance of the maximum standardized uptake value (SUVmax) prior to treatment in 43 patients with colon cancer and unresectable liver metastases. Patients with colon cancer who underwent (18)F-FDG-PET/computed tomography (CT) scans for staging before the start of first-line 5-fluorouracil-based chemotherapy were retrospectively analyzed. Expression of Beclin-1 in cancer cells was evaluated in primary tumors using immunohistochemical staining...
February 28, 2017: Bioscience Trends
https://www.readbyqxmd.com/read/28243617/data-supporting-the-inability-of-indomethacin-to-induce-autophagy-in-u251-glioma-cells
#19
Aleksandar Pantovic, Katarina Arsikin, Milica Kosic, Biljana Ristic, Vladimir Trajkovic, Ljubica Harhaji-Trajkovic
Autophagy, a catabolic process involving intracellular degradation of unnecessary or dysfunctional cellular components through the lysosomal machinery, could act as a prosurvival, as well as a cytotoxic mechanism (Parzych and Klionsky, 2014) [1]. Cyclooxygenase inhibitor indomethacin inhibits proliferation of glioma cells, and has been reported to reduce the activity of the main autophagy repressor mammalian target of rapamycin (mTOR) (Pantovic et al., 2016) [2]. Here we investigated the ability of indomethacin to induce autophagy in U251 human glioma cells...
April 2017: Data in Brief
https://www.readbyqxmd.com/read/28243129/polydatin-regulates-proliferation-apoptosis-and-autophagy-in-multiple-myeloma-cells-through-mtor-p70s6k-pathway
#20
Baojun Yang, Shunxin Zhao
BACKGROUND: Polydatin (PD) plays an important role in suppressing platelet aggregation, reducing blood lipid, restoring microcirculation and protecting from myocardial ischemia/reperfusion injury and shock. In addition, PD possesses anticancer activity. However, the effect and the mechanism of PD in regulating multiple myeloma (MM) cell survival and death are still unknown. METHODS: Cell proliferation and apoptosis of RPMI 8226 cells, respectively, were analyzed by cell counting kit8 (CCK-8) assay and flow cytometry...
2017: OncoTargets and Therapy
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