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Goel, Manisha

Vineeta Kaushik, Ved Vrat Verma, Manisha Goel
Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated (CRISPR-Cas) systems present in genomes of bacteria and archaea have been the focus of many research studies recently. The Cas4 proteins of these systems are thought to be responsible for the adaptation step in the CRISPR mechanism. Cas4 proteins exhibit low sequence similarity among themselves and are currently classified into 2 main classes: DUF83 and DUF911. The characteristic features of Cas4 proteins belonging to DUF83 class have been elucidated by determining the structures of Cas4 protein from Sulfolobus solfataricus and Pyrobaculum calidifontis...
December 15, 2017: Journal of Molecular Recognition: JMR
Archana Sharma, Shikha Rani, Manisha Goel
CsaA is a protein involved in the post-translational translocation of proteins across the cytoplasmic membrane. It is considered to be a functional homolog of SecB which participates in the Sec-dependent translocation pathway in an analogous manner. CsaA has also been reported to act as a molecular chaperone, preventing aggregation of unfolded proteins. It is essentially a prokaryotic protein which is absent in eukaryotes, but found extensively in bacteria and earlier thought to be widely present in archaea...
September 18, 2017: Critical Reviews in Microbiology
Shikha Rani, Abhishikha Srivastava, Manish Kumar, Manisha Goel, Peter Lund
No abstract text is available yet for this article.
March 1, 2017: FEMS Microbiology Letters
Shobha Kumari, Ravi Kant Pal, Rani Gupta, Manisha Goel
Gamma glutamyl transpeptidase, (GGT) is a ubiquitous protein which plays a central role in glutathione metabolism and has myriad clinical implications. It has been shown to be a virulence factor for pathogenic bacteria, inhibition of which results in reduced colonization potential. However, existing inhibitors are effective but toxic and therefore search is on for novel inhibitors, which makes it imperative to understand the interactions of various inhibitors with the protein in substantial detail. High resolution structures of protein bound to different inhibitors can serve this purpose...
February 2017: Protein Journal
Anju Rastogi, Manisha Mishra, Yashpal Goel, Prolima Thacker, Kamlesh
PURPOSE: To compare 25- and 20-gauge pars plana vitrectomy (PPV) for the management of pediatric cataract. METHODS: 20 eyes of 15 patients were randomly divided into two groups to undergo pars plana capsulotomy and vitrectomy by either 25-gauge (group A) or 20-gauge (group B) PPV after lens aspiration and IOL implantation. The two groups were compared for total surgical time, time taken in doing pars plana capsulotomy and vitrectomy, and the size of posterior capsulotomy...
January 6, 2017: International Ophthalmology
Sandeep Kumar, Kavish Kr Jain, Shikha Rani, Kailash N Bhardwaj, Manisha Goel, Ramesh Chander Kuhad
Among lignocellulolytic enzymes, laccases are the most versatile, broadly specific, and largely studied enzyme with a wide range of biotechnological potential. Putative laccase (CotA) from Bacillus pumilus MK001 was cloned and expressed in E. coli. In addition to soluble bioactive fraction, inactive inclusion body fraction was also harvested and refolded under optimized conditions resulting in 64 % of refolding efficiency. The enzyme was found to be thermostable exhibiting a half-life of 60 min at 80 °C...
December 2016: Molecular Biotechnology
Yogesh Singh, Namita Gupta, Ved Vrat Verma, Manisha Goel, Rani Gupta
TALipB (33 kDa) is a solvent stable, enantioselective lipase from Trichosporon asahii MSR54. It is cysteine-rich and shows activation in presence of thiol reducing agents. DIANNA server predicted three disulphide bridges C53-C195 (S1), C89-C228 (S2) and C164-C254 (S3) in the enzyme. Selective disruption of disulphide bonds by cysteine to alanine mutations at Cys53 and Cys89 of S1 and S2 bonds resulted in enzyme activation. Mutant mTALipB (S1+S2) showed increase in specific activity by ∼4-fold (834 mM/mg) and improved Vmax of 6...
March 25, 2016: Biochemical and Biophysical Research Communications
Shikha Rani, Abhishikha Srivastava, Manish Kumar, Manisha Goel
Chaperones are a diverse class of ubiquitous proteins that assist other cellular proteins in folding correctly and maintaining their native structure. Many different chaperones cooperate to constitute the 'proteostasis' machinery in the cells. It has been proposed earlier that archaeal organisms could be ideal model systems for deciphering the basic functioning of the 'protein folding machinery' in higher eukaryotes. Several chaperone families have been characterized in archaea over the years but mostly one protein at a time, making it difficult to decipher the composition and mechanistics of the protein folding system as a whole...
March 2016: FEMS Microbiology Letters
Manisha Kumar, Usha Gupta, Jayashree Bhattacharjee, Ritu Singh, Shalini Singh, Manish Goel, Karuna Sharma, Mujeeb U Rehman
OBJECTIVE: To evaluate the role of maternal factors, biomarkers, and uterine-artery Doppler in the prediction of hypertension during pregnancy. METHODS: A prospective cohort study was performed between December 2012 and November 2014. All singleton pregnancies between 11 weeks and 13 weeks, 6 days of pregnancy were included. Patients had their body mass index (BMI, calculated as weight in kilograms divided by the square of height in meters), mean arterial pressure, uterine-artery Doppler pulsatility index, and pregnancy-associated plasma protein A (PAPP-A) and free β-human chorionic gonadotropin levels recorded...
February 2016: International Journal of Gynaecology and Obstetrics
Manisha Dixit, Ashutosh Raghuvanshi, Chandra Prakash Gupta, Jyoti Kureel, Mohd Nizam Mansoori, Priyanka Shukla, Aijaz A John, Kavita Singh, Dipak Purohit, Pallavi Awasthi, Divya Singh, Atul Goel
We evaluated the bone regeneration and healing effect of Medicarpin (med) in cortical bone defect model that heals by intramembranous ossification. For the study, female Sprague-Dawley rats were ovariectomized and rendered osteopenic. A drill hole injury was generated in mid femoral bones of all the animals. Med treatment was commenced the day after and continued for 15 days. PTH was taken as a reference standard. Fifteen days post-treatment, animals were sacrificed. Bones were collected for histomorphometry studies at the injury site by micro-computed tomography (μCT) and confocal microscopy...
2015: PloS One
Ved Vrat Verma, Rani Gupta, Manisha Goel
BACKGROUND: γ-glutamyltranspeptidase (GGT) is a bi-substrate enzyme conserved in all three domains of life. It catalyzes the cleavage and transfer of γ-glutamyl moiety of glutathione to either water (hydrolysis) or substrates like peptides (transpeptidation). GGTs exhibit great variability in their enzyme kinetics although the mechanism of catalysis is conserved. Recently, GGT has been shown to be a virulence factor in microbes like Helicobacter pylori and Bacillus anthracis. In mammalian cells also, GGT inhibition prior to chemotherapy has been shown to sensitize tumors to the therapy...
September 14, 2015: Biology Direct
Abhishikha Srivastava, Neelja Singhal, Manisha Goel, Jugsharan Singh Virdi, Manish Kumar
β-Lactam antibiotics are among the most widely used antibiotics against microbial pathogens. However, enzymatic hydrolysis of these antibiotics by bacterial β-lactamases is increasingly compromising their efficiency. Although new generation β-lactam antibiotics have been developed to combat antibiotic resistance, β-lactamases have also evolved along with the new variants of the substrate. A strong selection pressure from the newer generation of β-lactam antibiotics has resulted in evolution of different families within each class of β-lactamase...
2014: Database: the Journal of Biological Databases and Curation
Abhishikha Srivastava, Neelja Singhal, Manisha Goel, Jugsharan Singh Virdi, Manish Kumar
Beta-lactamases are a superfamily of enzymes which degrade the β-lactam class of antibiotics. They are produced endogenously by the bacterial cells, which when exposed to the β-lactam class of antibiotics inactivate them by cleaving the β-lactam ring. Based on the presence or absence of metallic ligand, β-lactamases have been divided into two broad functional classes. β-Lactamases are a constitutively evolving and expanding superfamily of enzymes, which could be further subdivided on the basis of presence/absence of conserved motifs...
2014: TheScientificWorldJournal
Anupama Goel, Sandhya Mittal, Bk Taneja, Manisha Singhal
OBJECTIVE: To study the efficacy of a low dose of mifepristone (100 mg) in combination with misoprostol, in women undergoing medical termination of pregnancy up to gestation of 49 days. MATERIAL AND METHODS: A prospective study was performed in 50 women (mean age 26.54±3.68 years) with single intrauterine pregnancy of up to 49 days of gestation, presenting to our institution between November 2007 and October 2009. 100 mg mifepristone was given orally, followed 24 hours later by 400 micrograms misoprostol vaginally...
2010: Journal of the Turkish German Gynecological Association
Piyush Verma, Manisha Pal, Ashwani Goel, Ishwar Singh, Virad Bansal
We present a rare case of the congenital malformation of the nose in a 10-month-old baby.
July 2011: Indian Journal of Otolaryngology and Head and Neck Surgery
Bhawna Arya, Parul R Chopra, Manisha Goel, Anuj Sharma, Anurag Krishna, Samiran Nundy
UNLABELLED: BACKGROUND; The announcement of the annual Padma awards in January always generates a great deal of interest as well as controversy and, some believe that many good candidates are excluded and many less deserving ones included. We analysed the recipients in the field of medicine to determine whether or not a pattern emerged regarding who were bestowed these honours. We were not able to objectively evaluate whether or not the honours were 'deserved'. METHODS: We obtained and then analysed the list of awardees from newspapers and the official website of the Ministry of Home Affairs...
November 2010: National Medical Journal of India
Manisha Jana, Arun K Gupta, Kundum R Prasad, Sandeep Goel, Vishal D Tambade, Upasna Sinha
Congenital anomalies of the male urogenital tract are common. Some lesions like posterior urethral valve or anterior urethral diverticulum tend to present early in infancy and are often easily diagnosed on conventional contrast voiding cystourethrograms. Other conditions like posterior urethral diverticulum or utricle can be relatively asymptomatic and therefore present late in childhood. We present the spectrum of imaging findings of common and uncommon anomalies involving the male urethra. Since the pediatric radiologist is often the first to make the diagnosis, he or she should be well aware of these conditions...
January 2011: Indian Journal of Radiology & Imaging
Inder Pal Singh Kapoor, Manisha Kapoor, Gurdip Singh, Udai P Singh, Nidhi Goel
Nitrate and perchlorate salts of 2,4,6-trimethylaniline have been prepared and characterized by X-ray crystallography and gravimetric analyses. Their thermal decomposition has been studied by TG, TG-DSC and ignition/explosion delays. It has been observed that proton transfer from substituted anilinium ion to nitrate and perchlorate ion regenerate amine, HNO(3) and HClO(4) in condensed phase at higher temperature, where oxidation-reduction between amine and acids leads to ignition and explosion. The kinetics of thermal decomposition was evaluated by applying model fitting as well as isoconversional methods...
January 15, 2010: Journal of Hazardous Materials
Leonid Minakhin, Manisha Goel, Zhanna Berdygulova, Erlan Ramanculov, Laurence Florens, Galina Glazko, Valeri N Karamychev, Alexei I Slesarev, Sergei A Kozyavkin, Igor Khromov, Hans-W Ackermann, Michael Washburn, Arcady Mushegian, Konstantin Severinov
The genomes of two closely related lytic Thermus thermophilus siphoviruses with exceptionally long (approximately 800 nm) tails, bacteriophages P23-45 and P74-26, were sequenced completely. The P23-45 genome consists of 84,201 bp with 117 putative open reading frames (ORFs), and the P74-26 genome has 83,319 bp and 116 putative ORFs. The two genomes are 92% identical with 113 ORFs shared. Only 25% of phage gene product functions can be predicted from similarities to proteins and protein domains with known functions...
April 25, 2008: Journal of Molecular Biology
Dhruti Savalia, Lars F Westblade, Manisha Goel, Laurence Florens, Priscilla Kemp, Natalja Akulenko, Olga Pavlova, Julio C Padovan, Brian T Chait, Michael P Washburn, Hans-W Ackermann, Arcady Mushegian, Tarasii Gabisonia, Ian Molineux, Konstantin Severinov
A novel bacteriophage infecting Escherichia coli was isolated during a large-scale screen for bacteriophages that may be used for therapy of mastitis in cattle. The 77,554-bp genome of the bacteriophage, named phiEco32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phiEco32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF family sigma factor that may be responsible for transcription of some viral genes...
March 28, 2008: Journal of Molecular Biology
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