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https://www.readbyqxmd.com/read/29326222/genome-sequence-of-actinobacillus-seminis-strain-atcc-15768-a-reference-strain-of-ovine-pathogens-that-causes-infections-in-reproductive-organs
#1
Erasmo Negrete-Abascal, Fernando Montes-Garcia, Sergio Vaca-Pacheco, Abraham M Leyto-Gil, Edgar Fragoso-Garcia, Roberto Carvente-Garcia, Sandra Perez-Agueros, Hugo G Castelan-Sanchez, Alejandra Garcia-Molina, Tomas E Villamar, Patricia Sánchez-Alonso, Candelario Vazquez-Cruz
The draft genome sequence of Actinobacillus seminis strain ATCC 15768 is reported here. The genome comprises 22 contigs corresponding to 2.36 Mb with 40.7% G+C content and contains several genes related to virulence, including a putative RTX protein.
January 11, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29321287/isolation-of-xenopus-oocytes
#2
Karen Newman, Tristan Aguero, Mary Lou King
Xenopus oocytes and oocyte extracts are the starting material for a variety of experimental approaches. Oocytes are obtained by surgical removal of the ovary from anesthetized females. Although oocytes may be used while they remain within their ovarian follicle, it is more practical to work with defolliculated oocytes. Defolliculation can be performed either manually or enzymatically. Here we present a protocol for the isolation and separation of Xenopus oocytes at various developmental stages, and guidelines for maintaining oocytes in culture...
January 10, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29321284/microinjection-of-xenopus-oocytes
#3
Tristan Aguero, Karen Newman, Mary Lou King
Microinjection of Xenopus oocytes has proven to be a valuable tool in a broad array of studies that require expression of DNA or RNA into functional protein. These studies are diverse and range from expression cloning to receptor-ligand interaction to nuclear programming. Oocytes offer a number of advantages for such studies, including their large size (∼1.2 mm in diameter), capacity for translation, and enormous nucleus (0.3-0.4 mm). They are cost effective, easily manipulated, and can be injected in large numbers in a short time period...
January 10, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29282554/soccer-helps-build-strong-bones-during-growth-a-systematic-review-and-meta-analysis
#4
REVIEW
Gabriel Lozano-Berges, Ángel Matute-Llorente, Alejandro González-Agüero, Alejandro Gómez-Bruton, Alba Gómez-Cabello, Germán Vicente-Rodríguez, José A Casajús
The aim of this study was to analyze the effects of soccer practice on bone in male and female children and adolescents. MEDLINE, PubMed, SPORTDiscus and Web of Science databases were searched for scientific articles published up to and including October 2016. Twenty-seven studies were included in this systematic review (13 in the meta-analysis). The meta-analysis was performed by using OpenMeta[Analyst] software. It is well documented that soccer practice during childhood provides positive effects on bone mineral content (BMC) and density (BMD) compared to sedentary behaviors and other sports, such as tennis, weightlifting, or swimming...
December 28, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/29280666/-bone-metabolism-in-child-and-adolescent-athletes-a-systematic-review
#5
Marta Rapún López, Hugo Olmedillas, Francisco Pradas de la Fuente, Alba Gómez-Cabello, Alejandro González-Agüero, José Antonio Casajús, Germán Vicente-Rodríguez
BACKGROUND: Physical exercise is beneficial for the prevention of osteoporosis. Bone metabolism markers are the main indicators of metabolic balance and may indicate early risk in bone tissue. AIM: This systematic review aims to summarize and update present knowledge about bone metabolism markers in child and adolescent athletes. METHOD: Literature search was conducted up to March 2017 in PubMed and Sport Discus. A total of 19 studies (case-control, longitudinal and randomized control trials) met the inclusion criteria and were included in the review...
November 16, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29280664/-role-of-the-stevia-and-l-carnitine-of-a-nutritional-supplement-on-glycemic-impact-in-adults
#6
Lissé Angarita Dávila, Samuel Durán Agüero, Daniel Apaicio, Karla Parra, Maria Uzcátegui, Virginia Céspedes, Nadia Reina Villasmil, José López Miranda
Currently the food industry has generated interest in non-nutritive sweeteners, for example Stevia and in special components such as L-carnitine, used in formulations of nutritional supplements for glycemic control specific for diabetics. The present study evaluated the effect of stevia and L-carnitine on the glycemic index (GI) and glycemic load (CG) of a nutritional supplement in 19 healthy subjects (9 men and 10 women), who randomly completed 3 consumption tests, 1 for the supplement and 1 for each reference product: Glucose solution (SG) and white bread (PB), obtaining blood samples at the 0, 15, 30, 45, 60, 90 and 120 min times; for measurement of blood glucose, basal and postprandial insulin...
November 16, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29280645/-content-validity-of-food-quality-survey-of-elderly-fqse
#7
Samuel Durán Agüero, Priscila Candia, Rafael Pizarro Mena
BACKGROUND: There are few instruments that evaluate the quality of food of elderly (ED) in the community. OBJECTIVE: To carry out the content validity of the survey designed to determine the Food Quality Survey of Elderly (FQSE). MATERIAL AND METHODS: Based on the existing literature, a questionnaire was designed to evaluate the FQSE. Content validity was performed using the Content Validity Index through the calculation of the Content Validity Ratio of Lawshe...
November 14, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29259975/relationship-between-smoking-and-acute-mountain-sickness-a-meta-analysis-of-observational-studies
#8
REVIEW
Cristina Masuet-Aumatell, Alba Sánchez-Mascuñano, Fernando Agüero Santangelo, Sergio Morchón Ramos, Josep Maria Ramon-Torrell
Aims: Previous epidemiological investigations of the relationship between smoking and acute mountain sickness (AMS) risk yielded inconsistent findings. Therefore, a meta-analysis of observational studies was performed to determine whether smoking is related to the development of AMS. Methods: Searches were performed on PubMed, Scopus, Embase, and Web of Science for relevant studies that were published before November 2016 reporting smoking prevalence and AMS. Two evaluators independently selected studies, extracted data, and assessed study quality...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29216983/atrial-infarction-and-ischemic-mitral-regurgitation-contribute-to-post-mi-remodeling-of-the-left-atrium
#9
Jaume Aguero, Carlos Galan-Arriola, Rodrigo Fernandez-Jimenez, Javier Sanchez-Gonzalez, Nina Ajmone, Victoria Delgado, Jorge Solis, Gonzalo J Lopez, Antonio de Molina-Iracheta, Roger J Hajjar, Jeroen J Bax, Valentin Fuster, Borja Ibáñez
BACKGROUND: Left atrial (LA) remodeling after an acute myocardial infarction (MI) is poorly characterized regarding its determinants or its effect on ischemic mitral regurgitation (MR) development. OBJECTIVES: The purpose of this study was: 1) to compare LA structural remodeling in experimental MI swine models recapitulating the effects of left ventricular (LV) dysfunction, ischemic MR, and left atrial infarction (LAI); and 2) to analyze how LA remodeling influences ischemic MR development...
December 12, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/29177949/prognostic-value-of-cd45-transcriptional-expression-in-head-and-neck-cancer
#10
Mercedes Camacho, Adriana Agüero, Anna Sumarroca, Laura López, Miguel-Ángel Pavón, Francesc-Xavier Avilés-Jurado, Jacinto García, Miquel Quer, Xavier León
INTRODUCTION: Tumor-infiltrating lymphocytes (TILs) have a recognized antitumor activity in head and neck squamous cell carcinoma (HNSCC). CD45 is one of the most highly expressed proteins in lymphocytes. We carry out a study to assess the prognostic value of transcriptional expression of CD45 in HNSCC. MATERIAL AND METHODS: We determined the transcriptional expression of CD45 in 160 consecutive HNSCC patients and compared the TIL values according to the CD45 expression...
November 25, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/29167301/prevalence-of-persistent-blood-eosinophilia-relation-to-outcomes-in-patients-with-copd
#11
Ciro Casanova, Bartolome R Celli, Juan P de-Torres, Cristina Martínez-Gonzalez, Borja G Cosio, Victor Pinto-Plata, Pilar de Lucas-Ramos, Miguel Divo, Antonia Fuster, Germán Peces-Barba, Myriam Calle-Rubio, Ingrid Solanes, Ramón Aguero, Nuria Feu-Collado, Inmaculada Alfageme, Alfredo De Diego, Amparo Romero, Eva Balcells, Antonia Llunell, Juan B Galdiz, Margarita Marin, Amalia Moreno, Carlos Cabrera, Rafael Golpe, Celia Lacarcel, Joan B Soriano, José Luis López-Campos, Juan J Soler-Cataluña, José M Marin
The impact of blood eosinophilia in chronic obstructive pulmonary disease (COPD) remains controversial.To evaluate the prevalence and stability of a high level of blood eosinophils (≥300 cells·μL-1) and its relationship to outcomes, we determined blood eosinophils at baseline and over 2 years in 424 COPD patients (forced expiratory volume in 1 s (FEV1) 60% predicted) and 67 smokers without COPD from the CHAIN cohort, and in 308 COPD patients (FEV1 60% predicted) in the BODE cohort. We related eosinophil levels to exacerbations and survival using Cox hazard analysis...
November 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29130710/body-fat-percentage-comparisons-between-four-methods-in-young-football-players-are-they-comparable
#12
Gabriel Lozano Berges, Ángel Matute Llorente, Alejandro Gómez Bruton, Alejandro González Agüero, German Vicente Rodríguez, José Antonio Casajús
INTRODUCTION: Dual X-ray absorptiometry (DXA), air displacement plethysmography (ADP), bioelectrical impedance analysis (BIA) and anthropometry are four body composition methods that have been frequently used for the assessment of body fat percentage (%BF) in athletes. However, the agreement between these methods has not been studied yet in adolescent football players. OBJECTIVES: The aim of this study was to compare %BF calculated by DXA, ADP, BIA and anthropometry in 92 participants...
October 24, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29130704/-relationship-between-anthropometric-health-indexes-with-food-consumption-in-physically-active-elderly
#13
Pablo Antonio Valdés-Badilla, Andrés Godoy-Cumillaf, Jenny Ortega-Spuler, Daniela Díaz-Aravena, Nibaldo Castro-Garrido, Luis Sandoval-Muñoz, Tomás Herrera-Valenzuela, Antonio López-Fuenzalida, Rodrigo Vargas-Vitoria, Samuel Durán-Aguero
INTRODUCTION: Programs focused on active aging do not always have actions to guide the elderly about healthy eating. Therefore, the concordance between the feeding habits and the morphological characteristics of this population group is little known. OBJECTIVE: To correlate the anthropometric health indexes with the frequency of food consumption in physically active elderly (PAE). METHODS: The sample consisted of 307 physically active Chilean elders of both sexes (8...
October 24, 2017: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
https://www.readbyqxmd.com/read/29130009/optical-coherence-tomography-angiography-of-two-choroidal-nevi-variants
#14
Victor M Villegas, Armando L Monroig, Lazaro H Aguero, Stephen G Schwartz
Optical coherence tomography angiography (OCT-A) is a recently established noninvasive technology for evaluation of the retinal and choroidal vasculature. The literature regarding the findings in choroidal nevi is scarce. We report the OCT-A findings associated with two different variants. Subject one had decreased vascular flow signal in the choroidal, choriocapillaris, deep retinal, and superficial retinal layers. Subject two had decreased vascular flow signal in the choroidal, choriocapillaris, and deep retinal layers with a normal vascular flow signal in the superficial retinal layer...
2017: Case Reports in Ophthalmological Medicine
https://www.readbyqxmd.com/read/29122333/translation-and-validation-of-the-multidimensional-dyspnea-12-questionnaire
#15
Carlos Antonio Amado Diago, Luis Puente Maestu, Beatriz Abascal Bolado, Juan Agüero Calvo, Mercedes Hernando Hernando, Irene Puente Bats, Ramón Agüero Balbín
INTRODUCTION: Dyspnea is a multidimensional symptom, but this multidimensionality is not considered in most dyspnea questionnaires. The Dyspnea-12 takes a multidimensional approach to the assessment of dyspnea, specifically the sensory and the affective response. The objective of this study was to translate into Spanish and validate the Dyspnea-12 questionnaire. METHODS: The original English version of the Dyspnea-12 questionnaire was translated into Spanish and backtranslated to analyze its equivalence...
November 6, 2017: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/29104235/assessment-of-prescribing-and-monitoring-habits-for-patients-taking-an-antiarrhythmic-and-concomitant-qtc-prolonging-antibiotic
#16
Kelsey Noss, Sandra M Aguero, Travis Reinaker
Patients may intermittently require antimicrobial therapy with a QTc-prolonging antibiotic, which presents a challenge for prescribers of patients already taking a QTc-prolonging antiarrhythmic. Manufacturers recommend close monitoring for evidence of QTc-prolongation with the concomitant use of QTc-prolonging medications, but the monitoring parameters are not well-defined. Previous studies recommend a surveillance electrocardiogram (EKG) be completed both before and after the initiation of QTc-prolonging medications, but it is unknown to what degree EKGs displaying the QTc-interval are used to alter physician order entry and pharmacist order verification during concomitant therapy...
November 1, 2017: Pharmacy (Basel, Switzerland)
https://www.readbyqxmd.com/read/29103357/noninvasive-liver-assessment-in-adult-patients-with-fontan-circulation-using-acoustic-radiation-force-impulse-elastography-and-hepatic-magnetic-resonance-imaging
#17
F Buendía-Fuentes, J L Melero-Ferrer, D Plaza-López, J Rueda-Soriano, A Osa-Saez, J Aguero, P Calvillo-Batllés, C Fonfria-Esparcia, A Ballesta-Cuñat, L Martí-Bonmatí, L Martínez-Dolz
BACKGROUND: Patients who have undergone the Fontan procedure are at risk of developing hepatic dysfunction. However, broad recommendations regarding liver monitoring are limited. The purpose of this study was to characterize the frequency of liver disease in adult Fontan patients using multimodality imaging (hepatic magnetic resonance imaging [MRI], acoustic radiation force impulse [ARFI] elastography, or hepatic ultrasound). METHODS: In a prospective cross-sectional analysis of adult patients palliated with a Fontan procedure, hepatic MRI, ARFI, and hepatic ultrasound were used to assess for liver disease...
January 1, 2017: World Journal for Pediatric & Congenital Heart Surgery
https://www.readbyqxmd.com/read/29101180/reduced-longitudinal-contraction-is-associated-with-ischemic-mitral-regurgitation-after-posterior-mi
#18
Kiyotake Ishikawa, Shin Watanabe, Nadjib Hammoudi, Jaume Aguero, Olympia Bikou, Kenneth Fish, Roger J Hajjar
BACKGROUND: The role of left ventricular (LV) longitudinal contraction in ischemic mitral regurgitation (MR) remains unclear. We hypothesized that reduced longitudinal contraction disrupts normal mitral valve plane displacement during systole and leads to mitral valve tethering, thereby inducing ischemic MR. METHODS: Twenty-three Yorkshire pigs underwent induction of different-sized posterior myocardial infarction (MI) using a percutaneous approach. Incidence of MR and its association to LV longitudinal strain was examined using speckle-tracking echocardiography at one month post-MI to determine their relationship...
November 3, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29082896/optimal-cut-off-points-of-fasting-and-post-glucose-stimulus-surrogates-of-insulin-resistance-as-predictors-of-metabolic-syndrome-in-adolescents-according-to-several-definitions
#19
Mónica Ivette Piña-Agüero, Aranza Zaldivar-Delgado, Alejandra Salas-Fernández, Azucena Martínez-Basila, Mariela Bernabe-García, Jorge Maldonado-Hernández
OBJECTIVE: The aim of this study was to determine optimal cut-off points of fasting and post-glucose stimulus surrogates of insulin resistance to predict metabolic syndrome in adolescents according to several definitions. METHODS: 155 adolescents living in Mexico City were enrolled during 2011 and 2012. Waist circumference and blood pressure were recorded. Subjects received an oral glucose load of 1.75g per kg up to a maximum dose of 75g. Blood samples were drawn at baseline and 120 minutes...
October 30, 2017: Journal of Clinical Research in Pediatric Endocrinology
https://www.readbyqxmd.com/read/29047495/minimum-variance-control-for-mitigation-of-vibrations-in-adaptive-optics-systems
#20
Pedro Escárate, Rodrigo Carvajal, Laird Close, Jared Males, Katie Morzinski, Juan C Agüero
In this paper, we address the design of a minimum variance controller (MVC) for the mitigation of vibrations in modern telescope adaptive optics (AO) systems. It is widely accepted that a main source of non-turbulent perturbations is the mechanical resonance induced by the wind or the instrumentation systems, such as fans and cooling pumps. To adequately mitigate vibrations, the application of frequency-based controllers has been considered in the past decade. In this work, we express the system model in terms of the tracking of a zero-input signal via the MVC...
July 1, 2017: Applied Optics
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