keyword
MENU ▼
Read by QxMD icon Read
search

contag

keyword
https://www.readbyqxmd.com/read/29727572/functional-dna-delivery-enabled-by-lipid-modified-charge-altering-releasable-transporters-carts
#1
Nancy L Benner, Katherine E Near, Michael H Bachmann, Christopher H Contag, Robert M Waymouth, Paul A Wender
Safe and effective DNA delivery systems are required to enable or enhance clinical strategies and research involving gene therapy and DNA vaccinations. To address this delivery problem, a series of charge-altering releasable transporters (CARTs) with varied lipid content were prepared and evaluated for plasmid DNA (pDNA) delivery into cultured cells. These lipid-modified CART co-oligomers were synthesized in only two steps via sequential organocatalytic ring-opening polymerization of lipid-containing cyclic carbonate monomers and morpholinone monomers...
May 4, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29675353/magnetic-particle-imaging-of-islet-transplantation-in-the-liver-and-under-the-kidney-capsule-in-mouse-models
#2
Ping Wang, Patrick W Goodwill, Prachi Pandit, Jeff Gaudet, Alana Ross, Junfeng Wang, Elaine Yu, Daniel W Hensley, Timothy C Doyle, Christopher H Contag, Steven Conolly, Anna Moore
Background: Islet transplantation (Tx) represents the most promising therapy to restore normoglycemia in type 1 diabetes (T1D) patients to date. As significant islet loss has been observed after the procedure, there is an urgent need for developing strategies for monitoring transplanted islet grafts. In this report we describe for the first time the application of magnetic particle imaging (MPI) for monitoring transplanted islets in the liver and under the kidney capsule in experimental animals...
March 2018: Quantitative Imaging in Medicine and Surgery
https://www.readbyqxmd.com/read/29610072/automated-cell-segmentation-for-quantitative-phase-microscopy
#3
Nathan O Loewke, Sunil Pai, Christine Cordeiro, Dylan Black, Bonnie L King, Christopher H Contag, Bertha Chen, Thomas M Baer, Olav Solgaard
Automated cell segmentation and tracking is essential for dynamic studies of cellular morphology, movement, and interactions as well as other cellular behaviors. However, accurate, automated, and easy-to-use cell segmentation remains a challenge, especially in cases of high cell densities, where discrete boundaries are not easily discernable. Here, we present a fully automated segmentation algorithm that iteratively segments cells based on the observed distribution of optical cell volumes measured by quantitative phase microscopy...
April 2018: IEEE Transactions on Medical Imaging
https://www.readbyqxmd.com/read/29410542/utility-of-prenatal-doppler-ultrasound-to-predict-neonatal-impaired-cerebral-autoregulation
#4
Sruthi R Polavarapu, Garrett D Fitzgerald, Stephen Contag, Suma B Hoffman
OBJECTIVE: Determine if abnormal prenatal Doppler ultrasound indices are predictive of postnatal impaired cerebral autoregulation. STUDY DESIGN: Prospective cohort study of 46 subjects, 240 -296 weeks' gestation. Utilizing near-infrared spectroscopy and receiver-operating characteristic analysis, impaired cerebral autoregulation was defined as >16.5% time spent in a dysregulated state within 96 h of life. Normal and abnormal Doppler indices were compared for perinatal outcomes...
February 6, 2018: Journal of Perinatology: Official Journal of the California Perinatal Association
https://www.readbyqxmd.com/read/29355061/the-impact-of-maternal-body-mass-index-on-external-cephalic-version-success
#5
Shahrukh Chaudhary, Stephen Contag, Ruofan Yao
OBJECTIVE: The purpose of this study is to determine the association between body mass index (BMI) and success of ECV. METHODS: This is a cross-sectional analysis of singleton live births in the USA from 2010 to 2014 using birth certificate data. Patients were assigned a BMI category according to standard WHO classification. Comparisons of success of ECV between the BMI categories were made using chi-square analysis with normal BMI as the reference group. Cochran-Armitage test was performed to look for a trend of decreasing success of ECV as BMI increased...
January 21, 2018: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29232355/proximal-junctional-kyphosis-inter-and-intraobserver-reliability-of-radiographic-measurements-in-adult-spinal-deformity
#6
Farbod Rastegar, Alec Contag, Alan Daniels, Jayme Hiratzka, Clifford Lin, Jason Chang, Khoi Than, Ahmed Raslan, Christopher Kong, Ngoc-Lam Nguyen, Richard Hostin, Marie Kane, Robert A Hart
STUDY DESIGN: Reliability study of radiographic measures of proximal junctional kyphosis (PJK) in patients with adult spinal deformity (ASD). OBJECTIVE: To assess impacts of level of proximal endpoint and vertebral fracture on reliability of measurement of junctional kyphosis. SUMMARY OF BACKGROUND DATA: Radiographic assessment is important in determining management of patients with PJK or proximal junctional failure (PJF). No study to date has evaluated the reliability of radiographic measurement of the junctional kyphotic angle after surgery for ASD...
January 1, 2018: Spine
https://www.readbyqxmd.com/read/29196622/intracellular-biomass-flocculation-as-a-key-mechanism-of-rapid-bacterial-killing-by-cationic-amphipathic-antimicrobial-peptides-and-peptoids
#7
Nathaniel P Chongsiriwatana, Jennifer S Lin, Rinki Kapoor, Modi Wetzler, Jennifer A C Rea, Maruti K Didwania, Christopher H Contag, Annelise E Barron
Many organisms rely on antimicrobial peptides (AMPs) as a first line of defense against pathogens. In general, most AMPs are thought to kill bacteria by binding to and disrupting cell membranes. However, certain AMPs instead appear to inhibit biomacromolecule synthesis, while causing less membrane damage. Despite an unclear understanding of mechanism(s), there is considerable interest in mimicking AMPs with stable, synthetic molecules. Antimicrobial N-substituted glycine (peptoid) oligomers ("ampetoids") are structural, functional and mechanistic analogs of helical, cationic AMPs, which offer broad-spectrum antibacterial activity and better therapeutic potential than peptides...
December 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29189643/inter-and-intra-rater-reliability-of-the-hart-issg-proximal-junctional-failure-severity-scale
#8
Robert A Hart, Farbod Rastegar, Alec Contag, Marie Kane, Alan Daniels, Eric Klineberg, Robert Eastlack, Justin S Smith, Richard Hostin, D Kojo Hamilton, Jeffrey L Gum, Douglas C Burton, Justin K Sheer, Christopher Ames, Frank Schwab, Virginie Lafage, Shay Bess, Christopher Shaffrey, Khaled Kebaish
STUDY DESIGN: Reliability/external validation study. OBJECTIVE: Investigate inter- and intra-rater reliability of the Hart-ISSG PJFSS and its correlation with operative revision in patients with proximal junctional failure (PJF). SUMMARY OF BACKGROUND DATA: The Hart-ISSG PJFSS is a validated classification system for PJF. Reliability of the PJFSS has not been assessed. METHODS: Sixteen detailed clinical scenarios were assessed using the ISSG PJFSS classification in six categories: neurologic status, axial pain, instrumentation issue, proximal kyphotic angle, level of upper instrumented vertebrae (UIV), and severity of UIV/UIV+1 fracture...
November 20, 2017: Spine
https://www.readbyqxmd.com/read/29172787/adverse-perinatal-outcomes-associated-with-trial-of-labor-after-cesarean-section-at-term-in-pregnancies-complicated-by-maternal-obesity
#9
Ruofan Yao, Sarah D Crimmins, Stephen A Contag, Jerome N Kopelman, Katherine R Goetzinger
INTRODUCTION: Obesity is associated with higher risks for intrapartum complications. Therefore, we sought to determine if trial of labor after cesarean section (TOLAC) will lead to higher maternal and neonatal complications compared to repeat cesarean section (RCD). METHODS: This was a retrospective cohort analysis of singleton nonanomalous births between 37 and 42 weeks GA complicated by maternal obesity (body mass index (BMI) ≥ 30 kg/m2 ) and history of one or two previous cesarean deliveries...
November 27, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/29141657/local-estrogen-axis-in-the-human-bone-microenvironment-regulates-estrogen-receptor-positive-breast-cancer-cells
#10
Derek F Amanatullah, John S Tamaresis, Pauline Chu, Michael H Bachmann, Nhat M Hoang, Deborah Collyar, Aaron T Mayer, Robert B West, William J Maloney, Christopher H Contag, Bonnie L King
BACKGROUND: Approximately 70% of all breast cancers express the estrogen receptor, and are regulated by estrogen. While the ovaries are the primary source of estrogen in premenopausal women, most breast cancer is diagnosed following menopause, when systemic levels of this hormone decline. Estrogen production from androgen precursors is catalyzed by the aromatase enzyme. Although aromatase expression and local estrogen production in breast adipose tissue have been implicated in the development of primary breast cancer, the source of estrogen involved in the regulation of estrogen receptor-positive (ER+) metastatic breast cancer progression is less clear...
November 15, 2017: Breast Cancer Research: BCR
https://www.readbyqxmd.com/read/29020448/preparation-of-tc99m-labeled-pseudomonas-bacteriophage-without-adversely-impacting-infectivity-or-biodistribution
#11
Derek Holman, Matthew P Lungren, Jonathan Hardy, Chris Contag, Francis Blankenberg
Bacteriophages (phages) are ubiquitous viruses which have adapted to infect and replicate within target bacteria, their only known hosts, in a strain specific fashion with minimal cross infectivity. The recent steep rise in antibiotic resistance throughout the world has renewed interest in adapting phages for the imaging and treatment of bacterial infection in humans. In this article, we describe the current limitations surrounding the radiolabeling of phage for the imaging and treatment of bacterial infection and methods to overcome these difficulties...
November 15, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28982129/the-tyrosine-kinase-inhibitor-imatinib-mesylate-suppresses-uric-acid-crystal-induced-acute-gouty-arthritis-in-mice
#12
Laurent L Reber, Philipp Starkl, Bianca Balbino, Riccardo Sibilano, Nicolas Gaudenzio, Stephan Rogalla, Steven Sensarn, Dongmin Kang, Harini Raghu, Jeremy Sokolove, William H Robinson, Christopher H Contag, Mindy Tsai, Stephen J Galli
Gouty arthritis is caused by the deposition of monosodium urate (MSU) crystals in joints. Despite many treatment options for gout, there is a substantial need for alternative treatments for patients unresponsive to current therapies. Tyrosine kinase inhibitors have demonstrated therapeutic benefit in experimental models of antibody-dependent arthritis and in rheumatoid arthritis in humans, but to date, the potential effects of such inhibitors on gouty arthritis has not been evaluated. Here we demonstrate that treatment with the tyrosine kinase inhibitor imatinib mesylate (imatinib) can suppress inflammation induced by injection of MSU crystals into subcutaneous air pouches or into the ankle joint of wild type mice...
2017: PloS One
https://www.readbyqxmd.com/read/28832465/in-reply
#13
Ruofan Yao, Katherine R Goetzinger, Sarah D Crimmins, Jerome N Kopelman, Stephen A Contag
No abstract text is available yet for this article.
September 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28631141/cell-labeling-with-magneto-endosymbionts-and-the-dissection-of-the-subcellular-location-fate-and-host-cell-interactions
#14
Kayla R Lee, Abdul Wakeel, Papia Chakraborty, Chandler S Foote, Lauren Kajiura, Joyce C Barrozo, Andrea C Chan, Alexey V Bazarov, Ryan Spitler, Peter M Kutny, Jim M Denegre, Rob A Taft, Joachim Seemann, Bradley W Rice, Christopher H Contag, Brian K Rutt, Caleb B Bell
PURPOSE: The purposes of this study are to characterize magneto-endosymbiont (ME) labeling of mammalian cells and to discern the subcellular fate of these living contrast agents. MEs are novel magnetic resonance imaging (MRI) contrast agents that are being used for cell tracking studies. Understanding the fate of MEs in host cells is valuable for designing in vivo cell tracking experiments. PROCEDURES: The ME's surface epitopes, contrast-producing paramagnetic magnetosomal iron, and genome were studied using immunocytochemistry (ICC), Fe and MRI contrast measurements, and quantitative polymerase chain reaction (qPCR), respectively...
February 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28616842/characterization-of-magneto-endosymbionts-as-mri-cell-labeling-and-tracking-agents
#15
Kimberly D Brewer, Ryan Spitler, Kayla R Lee, Andrea C Chan, Joyce C Barrozo, Abdul Wakeel, Chandler S Foote, Steven Machtaler, James Rioux, Juergen K Willmann, Papia Chakraborty, Bradley W Rice, Christopher H Contag, Caleb B Bell, Brian K Rutt
PURPOSE: Magneto-endosymbionts (MEs) show promise as living magnetic resonance imaging (MRI) contrast agents for in vivo cell tracking. Here we characterize the biomedical imaging properties of ME contrast agents, in vitro and in vivo. PROCEDURES: By adapting and engineering magnetotactic bacteria to the intracellular niche, we are creating magneto-endosymbionts (MEs) that offer advantages relative to passive iron-based contrast agents (superparamagnetic iron oxides, SPIOs) for cell tracking...
February 2018: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28614512/sexual-and-reproductive-health-among-young-people-rio-de-janeiro-brazil
#16
Stella Regina Taquette, Denise Leite Maia Monteiro, Nádia Cristina Pinheiro Rodrigues, Riva Rozenberg, Daniela Contage Siccardi Menezes, Adriana de Oliveira Rodrigues, José Augusto Sapienza Ramos
We aimed to analyze the geographic distribution, the structure of healthcare services and the human resources of all units of the Sistema Único de Saúde (SUS - the Unified Health System) that provide sexual and reproductive health (SRH) services to the adolescent population in the second largest city in Brazil. We conducted a cross-sectional study with geographical mapping and data collection through a questionnaire applied in person with coordinators of the units or their representatives in 147 outpatient clinics in Rio de Janeiro that have SSR services...
June 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28591078/proximal-junctional-kyphosis-inter-and-intra-observer-reliability-of-radiographic-measurements-in-adult-spinal-deformity
#17
Farbod Rastegar, Alec Contag, Alan Daniels, Jayme Hiratzka, Clifford Lin, Jason Chang, Khoi Than, Ahmed Raslan, Christopher Kong, Ngoc-Lam Nguyen, Richard Hostin, Marie Kane, Robert Hart
STUDY DESIGN: Reliability study of radiographic measures of proximal junctional kyphosis in adult spinal deformity patients. OBJECTIVE: Assess impacts of level of proximal endpoint and vertebral fracture on reliability of measurement of junctional kyphosis. SUMMARY OF BACKGROUND DATA: Radiographic assessment is important in determining management of patients with Proximal Junctional Kyphosis (PJK) or Proximal Junctional Failure (PJF). No study to date has evaluated the reliability of radiographic measurement of the junctional kyphotic angle after surgery for Adult Spinal Deformity (ASD)...
June 6, 2017: Spine
https://www.readbyqxmd.com/read/28490473/specific-imaging-of-bacterial-infection-using-6%C3%A2-18-f-fluoromaltotriose-a-second-generation-pet-tracer-targeting-the-maltodextrin-transporter-in-bacteria
#18
Gayatri Gowrishankar, Jonathan Hardy, Mirwais Wardak, Mohammad Namavari, Robert E Reeves, Evgenios Neofytou, Ananth Srinivasan, Joseph C Wu, Christopher H Contag, Sanjiv Sam Gambhir
6″-18 F-fluoromaltotriose is a PET tracer that can potentially be used to image and localize most bacterial infections, much like 18 F-FDG has been used to image and localize most cancers. However, unlike 18 F-FDG, 6″-18 F-fluoromaltotriose is not taken up by inflammatory lesions and appears to be specific to bacterial infections by targeting the maltodextrin transporter that is expressed in gram-positive and gram-negative strains of bacteria. Methods: 6″-18 F-fluoromaltotriose was synthesized with high radiochemical purity and evaluated in several clinically relevant bacterial strains in cultures and in living mice...
October 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28437855/mild-fetal-cerebral-ventriculomegaly-prevalence-characteristics-and-utility-of-ancillary-testing-in-cases-presenting-to-a-tertiary-referral-center
#19
Alison J Mehlhorn, Cara E Morin, Jade J Wong-You-Cheong, Stephen A Contag
OBJECTIVE: Ventriculomegaly is the most common fetal brain anomaly identified during prenatal anatomy ultrasound. The aim of our study was to characterize cases of mild ventriculomegaly and investigate the utility of ancillary tests. METHOD: We reviewed 121 cases of mild ventriculomegaly, defined as lateral ventricle diameter of 10 to 15 mm. Characteristics of the ventricular dilation as well as each pregnancy were investigated. Ancillary tests performed included follow-up magnetic resonance imaging (MRI), chromosomal abnormality testing, and maternal serologic infection screening...
July 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28428283/regulatory-aspects-of-optical-methods-and-exogenous-targets-for-cancer-detection
#20
Willemieke S Tummers, Jason M Warram, Kiranya E Tipirneni, John Fengler, Paula Jacobs, Lalitha Shankar, Lori Henderson, Betsy Ballard, Brian W Pogue, Jamey P Weichert, Michael Bouvet, Jonathan Sorger, Christopher H Contag, John V Frangioni, Michael F Tweedle, James P Basilion, Sanjiv S Gambhir, Eben L Rosenthal
Considerable advances in cancer-specific optical imaging have improved the precision of tumor resection. In comparison to traditional imaging modalities, this technology is unique in its ability to provide real-time feedback to the operating surgeon. Given the significant clinical implications of optical imaging, there is an urgent need to standardize surgical navigation tools and contrast agents to facilitate swift regulatory approval. Because fluorescence-enhanced surgery requires a combination of both device and drug, each may be developed in conjunction, or separately, which are important considerations in the approval process...
May 1, 2017: Cancer Research
keyword
keyword
103400
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"