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Jesper Larsen, Paal S Andersen, Volker Winstel, Andreas Peschel
Background: CoNS species are likely reservoirs of the staphylococcal cassette chromosome mec (SCC mec ) in Staphylococcus aureus . S . aureus CC395 is unique as it is capable of exchanging DNA with CoNS via bacteriophages, which are also known to mediate transfer of SCC mec . Objectives: To analyse the structure and putative origin of the SCC mec element in S . aureus CC395. Methods: The only MRSA CC395 strain described in the literature, JS395, was subjected to WGS, and its SCC mec element was compared with those found in CoNS species and other S...
April 1, 2017: Journal of Antimicrobial Chemotherapy
Isabelle Bleiziffer, Julian Eikmeier, Gottfried Pohlentz, Kathryn McAulay, Guoqing Xia, Muzaffar Hussain, Andreas Peschel, Simon Foster, Georg Peters, Christine Heilmann
Most bacterial glycoproteins identified to date are virulence factors of pathogenic bacteria, i.e. adhesins and invasins. However, the impact of protein glycosylation on the major human pathogen Staphylococcus aureus remains incompletely understood. To study protein glycosylation in staphylococci, we analyzed lysostaphin lysates of methicillin-resistant Staphylococcus aureus (MRSA) strains by SDS-PAGE and subsequent periodic acid-Schiff's staining. We detected four (>300, ∼250, ∼165, and ∼120 kDa) and two (>300 and ∼175 kDa) glycosylated surface proteins with strain COL and strain 1061, respectively...
January 2017: PLoS Pathogens
Jialing Lin, Ping Xu, Yang Peng, Dongxin Lin, Qianting Ou, Ting Zhang, Chan Bai, Xiaohua Ye, Junli Zhou, Zhenjiang Yao
AIMS/INTRODUCTION: Evidence suggests that diabetes might cause an increase in colonization of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA) in community settings. We carried out a cross-sectional study to determine the prevalence and influencing factors of S. aureus and MRSA nasal colonization among a community-based diabetes population, and to identify the characteristics of the isolated strains. MATERIALS AND METHODS: A total of 956 participants from 11 community settings were included in the study...
November 3, 2016: Journal of Diabetes Investigation
Velma Rebic, Ana Budimir, Mufida Aljicevic, Sabaheta Bektas, Sabina Mahmutovic Vranic, Damir Rebic
BACKGROUND: Methicillin resistant Staphylococcus aureus (MRSA) is responsible for a wide spectrum of nosocomial and community associated infections worldwide. The aim of this study was to analyze MRSA strains from the general population in Canton Sarajevo, B&H. METHODS: Our investigation including either phenotypic and genotypic markers such as antimicrobial resistance, pulsed-field gel electrophoresis (PFGE), SCC typing, and Panton-Valentine leukocidin (PVL) detection...
July 16, 2016: Acta Informatica Medica: AIM
Stefan Monecke, Lutz Jatzwauk, Elke Müller, Hedda Nitschke, Katharina Pfohl, Peter Slickers, Annett Reissig, Antje Ruppelt-Lorz, Ralf Ehricht
SCCmec elements are very important mobile genetic elements in Staphylococci that carry beta-lactam resistance genes mecA/mecC, recombinase genes and a variety of accessory genes. Twelve main types and a couple of variants have yet been described. In addition, there are also other SCC elements harbouring other markers. In order to subtype strains of methicillin-resistant S. aureus (MRSA) based on variations within their SCCmec elements, 86 markers were selected from published SCC sequences for an assay based on multiplexed primer extension reactions followed by hybridisation to the specific probes...
2016: PloS One
H S Tunsjø, S Kalyanasundaram, M M Worren, T M Leegaard, A E F Moen
Rapid nucleic acid amplification tests for methicillin-resistant Staphylococcus aureus (MRSA) diagnostics commonly target the mec resistance gene, genes specific for S. aureus, and the integration site for the SCCmec resistance cassette, orfX. Due to poor specificity when these target genes are used individually, additional culture is required to verify positive results. The combination of these targets is useful, but the optimal algorithm may depend on the presence of the genetic markers in S. aureus isolates, as well as the prevalence of MRSA in a population...
January 2017: European Journal of Clinical Microbiology & Infectious Diseases
Ignacio Mir-Sanchis, Christina A Roman, Agnieszka Misiura, Ying Z Pigli, Susan Boyle-Vavra, Phoebe A Rice
Methicillin-resistant Staphylococcus aureus (MRSA) is a public-health threat worldwide. Although the mobile genomic island responsible for this phenotype, staphylococcal cassette chromosome (SCC), has been thought to be nonreplicative, we predicted DNA-replication-related functions for some of the conserved proteins encoded by SCC. We show that one of these, Cch, is homologous to the self-loading initiator helicases of an unrelated family of genomic islands, that it is an active 3'-to-5' helicase and that the adjacent ORF encodes a single-stranded DNA-binding protein...
October 2016: Nature Structural & Molecular Biology
L K Wilson, G W Coombs, K Christiansen, W B Grubb, F G O'Brien
BACKGROUND: In Western Australia (WA), clonal complex 5, ST835, community-associated (CA) MRSA is isolated almost exclusively from aged care facilities. In WA four different staphylococcal cassette chromosome (SCC) mec (SCCmec) elements have been identified in this ST, indicating high genetic activity in the SCCmec region. OBJECTIVES: To investigate the SCC region of ST835 CA-MRSA WA MRSA-40 and determine the distribution of an SCCsorbitol element found within the region...
August 26, 2016: Journal of Antimicrobial Chemotherapy
S Rajkhowa, D K Sarma, S R Pegu
Staphylococcus aureus is one of the most important pathogens of both humans and animal. Methicillin-resistant Staphylococcus aureus (MRSA) is an important human pathogen that causes serious infections both in hospitals and communities due to its multidrug resistance tendency. This study was undertaken to characterize the MRSA isolates from pigs and to determine the antimicrobial resistance of these isolates. Forty nine MRSA strains (one strain per positive pig) isolated from pigs of Northeast India were characterized by SCCmec typing and antimicrobial resistance...
December 2016: Veterinary Research Communications
Norhidayah Mat Azis, Hui P Pung, Abdul R Abdul Rachman, Syafinaz Amin Nordin, Seri N E Sarchio, Zarizal Suhaili, Mohd N Mohd Desa
The aim of the present study was to assess and compare the antimicrobial susceptibility pattern against a panel of antibiotics and molecular and methicillin resistance-associated genotypes of 120 carriage S. aureus isolates previously isolated from a student population at two isolation events within a one-month interval. The antibiotic susceptibility of isolates was determined using the Kirby-Bauer disc-diffusion method (cefoxitin by Etest). The MRSA was screened using polymerase chain reaction for the presence of the mecA gene...
March 2017: Journal of Infection and Public Health
Sarah L Baines, Benjamin P Howden, Helen Heffernan, Timothy P Stinear, Glen P Carter, Torsten Seemann, Jason C Kwong, Stephen R Ritchie, Deborah A Williamson
The prevalence of fusidic acid (FA) resistance amongStaphylococcus aureusstrains in New Zealand (NZ) is among the highest reported globally, with a recent study describing a resistance rate of approximately 28%. Three FA-resistantS. aureusclones (ST5 MRSA, ST1 MSSA, and ST1 MRSA) have emerged over the past decade and now predominate in NZ, and in all three clones FA resistance is mediated by thefusCgene. In particular, ST5 MRSA has rapidly become the dominant MRSA clone in NZ, although the origin of FA-resistant ST5 MRSA has not been explored, and the genetic context offusCin FA-resistant NZ isolates is unknown...
April 2016: Antimicrobial Agents and Chemotherapy
Ryuta Yonezawa, Tsukasa Kuwana, Kengo Kawamura, Yasuji Inamo
Pediatric invasive community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infection is very serious and occasionally fatal. This infectious disease is still a relatively rare and unfamiliar infectious disease in Japan. We report a positive outcome in a 23-month-old Japanese girl with meningitis, osteomyelitis, fasciitis, necrotizing pneumonia, urinary tract infection, and bacteremia due to CA-MRSA treated with linezolid. PCR testing of the CA-MRSA strain was positive for PVL and staphylococcal enterotoxin b and negative for ACME...
2015: Case Reports in Pediatrics
Abdullah Sarkar, Adeola Raji, Ghada Garaween, Olusegun Soge, Jose Rey-Ladino, Wael Al-Kattan, Atef Shibl, Abiola Senok
Most Staphylococcus aureus infections occur in previously colonized persons who also act as reservoirs for continued dissemination. This study aimed to investigate the carriage of antimicrobial resistance and virulence markers in S. aureus isolates associated with nasal colonization. The study was conducted from December 2013-April 2014. Nasal swabs were collected and questionnaires administered to 97 medical students in Riyadh Saudi Arabia. Bacterial culture, identification and antimicrobial susceptibility testing were performed by conventional methods and chromogenic agar was used for methicillin resistant S...
April 2016: Microbial Pathogenesis
T Tolosa, J Verbeke, S Piepers, M Tefera, Y Getachew, K Supré, S DeVliegher
Four studies were performed to quantify milk production, quality and consumption in the town Jimma, Ethiopia. First, 47 dairy farmers and 44 milk retailers were interviewed to gain more insights in dairy farming and marketing, and associated constraints. Second, bulk milk samples (n=188) were collected for 4 consecutive weeks to investigate milk quality [Total Bacterial Counts (TBC), Coliform Counts (CC), Somatic Cell Counts (SCC), and antimicrobial residues]. Third, (bulk) milk samples from 32 farms, 46 milk retailers and the 3 local milk collection centers were collected to determine the presence of oxacillin susceptible-and oxacillin resistant Staphylococcus aureus...
February 1, 2016: Preventive Veterinary Medicine
Maria Mazzilli, Renata Piccinini, Federico Scali, Alfonso Zecconi
Staphylococcus aureus isolated from mammary gland are characterized by different genetic patterns. Ninety four isolates from 33 dairy herds were analyzed by the means of a microarray to investigate S. aureus virulence patterns and the distribution of genes believed to be involved in immune evasion. None of the 94 isolates considered were MRSA. However, 50% of the isolates belonged to complexes related to MRSA and to human diseases, while only about 25% of them can be considered as exclusively of bovine origin...
December 2015: Research in Veterinary Science
Wei-Chun Hung, Hsiao-Jan Chen, Yu-Tzu Lin, Jui-Chang Tsai, Chiao-Wei Chen, Hsiao-Hung Lu, Sung-Pin Tseng, Yao-Yu Jheng, Kin Hong Leong, Lee-Jene Teng
We analyzed the occurrence and mechanisms of fusidic acid resistance present in staphylococci isolated from 59 healthy volunteers. The fingers of the volunteers were screened for the presence of staphylococci, and the collected isolates were tested for resistance to fusidic acid. A total of 34 fusidic acid resistant staphylococcal strains (all were coagulase-negative) were isolated from 22 individuals (22/59, 37.3%). Examination of the resistance genes revealed that acquired fusB or fusC was present in Staphylococcus epidermidis, Staphylococcus capitis subsp...
2015: PloS One
Cristiane Silveira de Brito, Lícia Ludendorff Queiroz, Paola Amaral de Campos, Deivid William da Fonseca Batistão, Helisângela de Almeida Silva, Guilherme Gularte de Agostini, Paulo Pinto Gontijo Filho, Rosineide Marques Ribas
INTRODUCTION: The frequency of methicillin-resistant Staphylococcus aureus (MRSA) has increased in the community. This study evaluated the prevalence of MRSA and community-acquired (CA)-MRSA in 120 healthy elderly. METHODS: The MRSA were evaluated for the presence of the IS256, mecA, agr, icaA, icaD, fnbB , and pvl genes with PCR. RESULTS: Frequency of S. aureus and MRSA colonization was 17.8% and 19%, respectively. CA-MRSA isolate showed SCC mec IV, fnbB+ , and icaD+ ...
September 2015: Revista da Sociedade Brasileira de Medicina Tropical
Paula Gómez, Carmen Lozano, Mari Cruz Camacho, José-Francisco Lima-Barbero, José-Manuel Hernández, Myriam Zarazaga, Úrsula Höfle, Carmen Torres
OBJECTIVES: The objective of this study was to analyse the prevalence of tracheal carriage of Staphylococcus aureus/MRSA in storks and to study the resistance and virulence genes in the obtained isolates. METHODS: Tracheal samples from 92 stork nestlings of two landfill-associated and two natural-habitat colonies were inoculated in specific media for S. aureus and MRSA recovery. Antimicrobial susceptibility was tested, and the presence of resistance, virulence and immune evasion cluster (IEC) genes was analysed by PCR...
January 2016: Journal of Antimicrobial Chemotherapy
Marwa I Abd El-Hamid, M M Bendary
The present study was designed to elucidate the phenotypic and genotypic characterization of S. aureus isolates in Egypt. The antibiotic susceptibility pattern of 133 identified S. aureus isolates revealed that over 70% of the isolates were multi-drug resistant (MDR). Fifty MDR isolates were characterized using antibiotyping and different molecular typing methods. Amplification of mecA gene confirmed 30 strains as methicillin-resistant S. aureus (MRSA) and 20 as methicillin-sensitive S. aureus (MSSA). Generally, 22 MRSA (73...
2015: Cellular and Molecular Biology
Marcela Rodriguez, Patrick G Hogan, Sarah W Satola, Emily Crispell, Todd Wylie, Hongyu Gao, Erica Sodergren, George M Weinstock, Carey-Ann D Burnham, Stephanie A Fritz
Historically, a number of typing methods have been evaluated for Staphylococcus aureus strain characterization. The emergence of contemporary strains of community-associated S. aureus, and the ensuing epidemic with a predominant strain type (USA300), necessitates re-evaluation of the discriminatory power of these typing methods for discerning molecular epidemiology and transmission dynamics, essential to investigations of hospital and community outbreaks. We compared the discriminatory index of 5 typing methods for contemporary S...
September 2015: Medicine (Baltimore)
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