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https://www.readbyqxmd.com/read/28062279/nitric-oxide-induced-synthesis-of-hydrogen-sulfide-alleviates-osmotic-stress-in-wheat-seedlings-through-sustaining-antioxidant-enzymes-osmolyte-accumulation-and-cysteine-homeostasis
#1
M Nasir Khan, M Mobin, Zahid Khorshid Abbas, Manzer H Siddiqui
Nitric oxide (NO) and hydrogen sulfide (H2S) have been shown to act as signaling molecules in various physiological processes, play significant roles in plant cellular processes, and also mediate responses to both biotic and abiotic stresses in plants. The present investigation was carried out to test the effect of exogenous NO on endogenous synthesis of H2S in osmotic-stressed wheat (Triticum aestivum L.) seedlings. The results show that application of NO to wheat seedlings, suffered from PEG8000-induced osmotic stress, considerably enhanced the activities of H2S-synthesizing enzymes l-cysteine desulfhydrase (LCD) and d-cysteine desulfhydrase (DCD) leading to enhanced level of endogenous H2S content...
January 3, 2017: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/28028384/retraction-mitigation-of-nacl-stress-by-arbuscular-mycorrhizal-fungi-through-the-modulation-of-osmolytes-antioxidants-and-secondary-metabolites-in-mustard-brassica-juncea-l-plants
#2
(no author information available yet)
[This retracts the article on p. 869 in vol. 7, PMID: 27458462.].
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28019048/temporal-and-spatial-changes-in-gene-expression-metabolite-accumulation-and-phytohormone-content-in-rice-seedlings-grown-under-drought-stress-conditions
#3
Daisuke Todaka, Yu Zhao, Takuya Yoshida, Madoka Kudo, Satoshi Kidokoro, Junya Mizoi, Ken-Suke Kodaira, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara, Kiminori Toyooka, Mayuko Sato, Alisdair R Fernie, Kazuo Shinozaki, Kazuko Yamaguchi-Shinozaki
To analyze the molecular mechanisms underlying plant responses to different levels of drought stress, we developed a soil matric potential (SMP)-based irrigation system that precisely controls soil moisture. Using this system, rice seedlings were grown under three different drought levels, denoted Md1, Md2 and Md3, with SMP values set to -9.8, -31.0, and -309.9 kPa, respectively. Although the Md1 treatment did not alter the visible phenotype, the Md2 treatment caused stomatal closure and shoot growth retardation (SGR)...
December 26, 2016: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28018415/a-halotolerant-bacterium-bacillus-licheniformis-hsw-16-augments-induced-systemic-tolerance-to-salt-stress-in-wheat-plant-triticum-aestivum
#4
Rajnish P Singh, Prabhat N Jha
Certain plant growth promoting bacteria can protect associated plants from harmful effects of salinity. We report the isolation and characterization of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase bacterium Bacillus licheniformis HSW-16 capable of ameliorating salt (NaCl) stress in wheat plants. The bacterium was isolated from the water of Sambhar salt lake, Rajasthan, India. The presence of ACC deaminase activity was confirmed by enzyme assay and analysis of AcdS gene, a structural gene for ACC deaminase...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28007169/uptake-and-transformation-of-arsenic-during-the-reproductive-life-stage-of-agaricus-bisporus-and-agaricus-campestris
#5
Michelle M Nearing, Iris Koch, Kenneth J Reimer
Fruiting bodies from the Agaricus genus have been found to contain non-toxic arsenobetaine (AB) as a major compound. It is unknown whether AB is formed during the vegetative or reproductive life stages of the fungus, or by the surrounding microbial community, but AB's structural similarity to glycine betaine has led to the hypothesis that AB may be adventitiously accumulated as an osmolyte. To investigate the potential formation of AB during the reproductive life stage of Agaricus species, growth substrate and fungi were collected during the commercial growth of Agaricus bisporus and analyzed for arsenic speciation using HPLC-ICP-MS...
November 2016: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/27994018/a-conserved-abc-transport-system-regulated-by-the-general-stress-response-pathways-of-alpha-and-gammaproteobacteria
#6
Julien Herrou, Jonathan W Willett, Daniel M Czyż, Gyorgy Babnigg, Youngchang Kim, Sean Crosson
: Brucella abortus σ(E1) is an EcfG-family sigma factor that regulates transcription of dozens of genes in response to diverse stress conditions, and is required for maintenance of chronic infection in a mouse model. A putative ATP-binding cassette transporter operon, bab1_0223-bab1_0226, is among the most highly activated gene sets in the σ(E1) regulon. The proteins encoded by this operon resemble quaternary ammonium compatible solute importers, but are most similar in sequence to the broadly conserved YehZYXW system, which remains largely uncharacterized...
December 19, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27992993/mechanisms-of-protein-translocation-on-dna-are-differentially-responsive-to-water-activity
#7
Brigitte S Naughton, Norbert O Reich
Water plays important but poorly understood roles in the functions of most biomolecules. We are interested in understanding how proteins use diverse search mechanisms to locate specific sites on DNA; here we present a study of the role of closely associated waters in diverse translocation mechanisms. The bacterial DNA adenine methyltransferase, Dam, moves across large segments of DNA using an intersegmental hopping mechanism, relying in part on movement through bulk water. In contrast, other proteins, such as the bacterial restriction endonuclease EcoRI, rely on a sliding mechanism, requiring the protein to stay closely associated with DNA...
December 20, 2016: Biochemistry
https://www.readbyqxmd.com/read/27989371/effects-of-high-salinity-from-desalination-brine-on-growth-photosynthesis-water-relations-and-osmolyte-concentrations-of-seagrass-posidonia-australis
#8
M L Cambridge, A Zavala-Perez, G R Cawthray, J Mondon, G A Kendrick
Highly saline brines from desalination plants expose seagrass communities to salt stress. We examined effects of raised salinity (46 and 54psu) compared with seawater controls (37psu) over 6weeks on the seagrass, Posidonia australis, growing in tanks with the aim of separating effects of salinity from other potentially deleterious components of brine and determining appropriate bioindicators. Plants survived exposures of 2-4weeks at 54psu, the maximum salinity of brine released from a nearby desalination plant...
December 15, 2016: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/27988118/effects-of-feeding-betaine-containing-liquid-supplement-to-transition-dairy-cows
#9
A P A Monteiro, J K Bernard, J-R Guo, X-S Weng, S Emanuele, R Davis, G E Dahl, S Tao
Betaine is a natural compound found in sugar beets that serves as a methyl donor and organic osmolyte when fed to animals. The objective was to evaluate the effect of feeding betaine-containing molasses on performance of transition dairy cows during late summer in 2 trials. In early September, cows were randomly assigned to betaine (BET) or control (CON) groups either shortly after dry off (trial 1; n = 10 per treatment) or 24 d before calving (trial 2; n = 8 per treatment) based on parity and previous mature equivalent milk yield...
December 14, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27981237/dipicolinic-acid-release-by-germinating-clostridium-difficile-spores-occurs-through-a-mechanosensing-mechanism
#10
Michael B Francis, Joseph A Sorg
Classically, dormant endospores are defined by their resistance properties, particularly their resistance to heat. Much of the heat resistance is due to the large amount of dipicolinic acid (DPA) stored within the spore core. During spore germination, DPA is released and allows for rehydration of the otherwise-dehydrated core. In Bacillus subtilis, 7 proteins are encoded by the spoVA operon and are important for DPA release. These proteins receive a signal from the activated germinant receptor and release DPA...
November 2016: MSphere
https://www.readbyqxmd.com/read/27942568/data-on-the-role-of-accessible-surface-area-on-osmolytes-induced-protein-stabilization
#11
Safikur Rahman, Syed Ausaf Ali, Asimul Islam, Md Imtaiyaz Hassan, Faizan Ahmad
This paper describes data related to the research article "Testing the dependence of stabilizing effect of osmolytes on the fractional increase in the accessible surface area on thermal and chemical denaturations of proteins" [1]. Heat- and guanidinium chloride (GdmCl)-induced denaturation of three disulfide free proteins (bovine cytochrome c (b-cyt-c), myoglobin (Mb) and barstar) in the presence of different concentrations of methylamines (sarcosine, glycine-betaine (GB) and trimethylamine-N-oxide (TMAO)) was monitored by [ϴ]222, the mean residue ellipticity at 222 nm at pH 7...
February 2017: Data in Brief
https://www.readbyqxmd.com/read/27935846/guardians-in-a-stressful-world-the-opu-family-of-compatible-solute-transporters-from-bacillus-subtilis
#12
Tamara Hoffmann, Erhard Bremer
The development of a semi-permeable cytoplasmic membrane was a key event in the evolution of microbial proto-cells. As a result, changes in the external osmolarity will inevitably trigger water fluxes along the osmotic gradient. The ensuing osmotic stress has consequences for the magnitude of turgor and will negatively impact cell growth and integrity. No microorganism can actively pump water across the cytoplasmic membrane; hence, microorganisms have to actively adjust the osmotic potential of their cytoplasm to scale and direct water fluxes in order to prevent dehydration or rupture...
February 1, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/27934218/mechanical-insight-into-resistance-of-betaine-to-urea-induced-protein-denaturation
#13
Jiantao Chen, Xiangjun Gong, Chaoxi Zeng, Yonghua Wang, Guangzhao Zhang
It is known that urea can induce protein denaturation that can be inhibited by osmolytes. Yet, experimental explorations on this mechanism at the molecular level are still lacking. We have investigated the resistance of betaine to the urea-induced denaturation of lysozyme in aqueous solutions using low-field NMR. Our study demonstrates that urea molecules directly interact with lysozyme, leading to denaturation. However, betaine molecules interacting with urea more strongly than lysozyme can pull the bound urea molecules from lysozyme so that the protein is protected from denaturation...
December 8, 2016: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/27933115/effects-of-trimethylamine-n-oxide-and-urea-on-dna-duplex-and-g-quadruplex
#14
Yu-Mi Ueda, Yu-Ki Zouzumi, Atsushi Maruyama, Shu-Ichi Nakano, Naoki Sugimoto, Daisuke Miyoshi
We systematically investigated effects of molecular crowding with trimethylamine N-oxide (TMAO) as a zwitterionic and protective osmolyte and urea as a nonionic denaturing osmolyte on conformation and thermodynamics of the canonical DNA duplex and the non-canonical DNA G-quadruplex. It was found that TMAO and urea stabilized and destabilized, respectively, the G-quadruplex. On the other hand, these osmolytes generally destabilize the duplex; however, it was observed that osmolytes having the trimethylamine group stabilized the duplex at the lower concentrations because of a direct binding to a groove of the duplex...
2016: Science and Technology of Advanced Materials
https://www.readbyqxmd.com/read/27915144/antioxidant-enzyme-and-osmotic-adjustment-changes-in-bean-seedlings-as-affected-by-biochar-under-salt-stress
#15
Salar Farhangi-Abriz, Shahram Torabian
Salinity damaged cellular membranes through overproduction of reactive oxygen species (ROS), while osmolytes and antioxidant capacities play a vital role in protecting plants from salinity caused oxidative damages. Biochar also could alleviate the negative impacts of salt stress in crops. The pot experiment was conducted to investigate the effects of biochar on some antioxidant enzyme activities and osmolyte adjustments of common bean (Phaseolus vulgaris L. cv. Derakhshan) under salinity stress. Bean plants were subjected to three salinity levels (non-saline, 6 and 12 dSm(-1) of NaCl) and biochar treatments (non-biochar, 10% and 20% total pot mass)...
March 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27904033/impact-of-slc6a-transporters-in-physiological-taurine-transport-at-the-blood-retinal-barrier-and-in-the-liver
#16
Yoshiyuki Kubo, Shin-Ichi Akanuma, Ken-Ichi Hosoya
Cumulative studies showed that taurine (2-aminoethanesulfonic acid) contributes to a variety of physiological events. Transport study suggested the cellular taurine transport in an Na(+)- and Cl(-)-dependent manner, and the several members of SLC6A family have been shown as taurine transporter. At the inner blood-retinal barrier (BRB), taurine transporter (TauT/SLC6A) is involved in the transport of taurine to the retina from the circulating blood. The involvement of TauT is also suggested in γ-aminobutyric acid (GABA) transport at the inner BRB, and its role is assumed in the elimination of GABA from the retinal interstitial fluid...
2016: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27902743/itraq-based-quantitative-proteomic-analysis-of-spirulina-platensis-in-response-to-low-temperature-stress
#17
Qingye Li, Rong Chang, Yijun Sun, Bosheng Li
Low temperature (LT) is one of the most important abiotic stresses that can significantly reduce crop yield. To gain insight into how Spirulina responds to LT stress, comprehensive physiological and proteomic analyses were conducted in this study. Significant decreases in growth and pigment levels as well as excessive accumulation of compatible osmolytes were observed in response to LT stress. An isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach was used to identify changes in protein abundance in Spirulina under LT...
2016: PloS One
https://www.readbyqxmd.com/read/27902449/is-n-n-dimethylglycine-n-oxide-a-choline-and-betaine-metabolite
#18
Michael Lever, Christopher J McEntyre, Peter M George, Stephen T Chambers
Choline metabolism is by oxidation to betaine, which is demethylated to N,N-dimethylglycine; dimethylglycine is oxidatively demethylated to sarcosine. This pathway is important for osmoregulation and as a source of methyl groups. We asked whether another metabolite was involved. We synthesized the N-oxide of dimethylglycine (DMGO) by oxidizing dimethylglycine with peracetic acid, and measured DMGO in human plasma and urine by HPLC-MS/MS with positive ion detection, using two chromatography procedures, based on ion exchange and HILIC separations...
January 13, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/27891145/contrasting-proteomic-and-metabolomic-responses-of-bermudagrass-to-drought-and-salt-stresses
#19
Tiantian Ye, Haitao Shi, Yanping Wang, Fan Yang, Zhulong Chan
Bermudagrass (Cynodon dactylon) is one of tolerant grass species to drought and salt. The comparative analyses of bermudagrass in response to drought and salt stresses at the physiological, proteomic, and metabolomic levels were performed in this study. The physiological results indicated that osmolytes accumulation, ROS level and antioxidant enzyme activities were extensively changed by drought and salt stresses. Through comparative proteomic analyses, we successfully identified a total of 77 proteins involved in photosynthesis, oxidative pentose phosphate, glycolysis, and redox metabolic pathways when exposed to drought and salt stresses...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27890800/metabolic-responses-of-haliotis-diversicolor-to-vibrio-parahaemolyticus-infection
#20
Jie Lu, Yanyan Shi, Shuhui Cai, Jianghua Feng
Vibrio parahemolyticus is a devastating bacterial pathogen that often causes outbreak of vibriosis in abalone Haliotis diversicolor. Elucidation of metabolic mechanisms of abalones in responding to V. parahemolyticus infection is essential for controlling the epidemic. In this work, (1)H NMR-based metabolomic techniques along with correlation and network analyses are used to investigate characteristic metabolites, as well as corresponding disturbed pathways in hepatopancreas and gill of H. diversicolor after V...
November 25, 2016: Fish & Shellfish Immunology
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