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https://www.readbyqxmd.com/read/28420180/synthesis-and-antiproliferative-activity-of-novel-all-trans-retinoic-acid-podophyllotoxin-conjugate-towards-human-gastric-cancer-cells
#1
Lei Zhang, Jing Wang, Lai Liu, Chengyue Zheng, Yang Wang
With the purpose of creating a multifunctional drug for gastric cancer treatment, a novel all-trans-retinoic acid (ATRA) conjugate with podophyllotoxin (PPT) was designed and synthesized, and its in vitro antiproliferative activity was evaluated against human gastric cancer cell lines using CCK-8 assay. The conjugate, P-A, exhibited significant anticancer activity against MKN-45 and BGC-823 cells with IC50 values of 0.419 ± 0.032 and 0.202 ± 0.055 μM, respectively. Moreover, P-A efficiently triggered cell cycle arrest and induced apoptosis in MKN-45 and BGC-823 cells due to modulation of cell cycle arrest- (CDK1, CDK2, CyclinA and CyclinB1) and apoptosis- (cleaved caspase-3, -8 and -9) related proteins, respectively...
April 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28394139/sequestration-of-guest-intermediates-by-dalesconol-bioassembly-lines-in-daldinia-eschscholzii
#2
Ai Hua Zhang, Wen Liu, Nan Jiang, Xin Lei Wang, Gang Wang, Qiang Xu, Ren Xiang Tan
Microbial constructions of secondary metabolites are generally biosynthetic gene cluster (BGC)-based, and the forging of different BGC-sourced intermediates tends to be overlooked. Here, we show that the dalesconol bioassembly lines in Daldinia eschscholzii can sequester guest intermediates (i.e., building blocks produced outside the dalesconol biosynthetic gene cluster) to form arrays of skeletally undescribed molecules such as (+)-dalescone A, a potent inhibitor against the NLRP3 inflammasome activation.
April 10, 2017: Organic Letters
https://www.readbyqxmd.com/read/28393452/minimization-of-the-thiolactomycin-biosynthetic-pathway-reveals-that-the-cytochrome-p450-enzyme-tlmf-is-required-for-five-membered-thiolactone-ring-formation
#3
Xiaoyu Tang, Jie Li, Bradley S Moore
Thiolactomycin (TLM) belongs to a class of rare and unique thiotetronate antibiotics inhibiting bacterial fatty acid synthesis. Although this group of natural product antibiotics was first discovered over 30 years, the study of TLM biosynthesis remains in its infancy. We recently discovered the biosynthetic gene cluster (BGC) for TLM from the marine bacterium Salinispora pacifica CNS-863. Here, we report the investigation of TLM biosynthetic logic through mutagenesis and comparative metabolic analyses. Our results reveal that only four genes (tlmF, tlmG, tlmH, and tlmI) are required for the construction of the characteristic γ-thiolactone skeleton of this class of antibiotics...
April 10, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28391286/basal-ganglia-calcification-with-tetanic-seizure-suggest-mitochondrial-disorder
#4
Josef Finsterer, Barbara Enzelsberger, Adam Bastowansky
BACKGROUND Basal ganglia calcification (BGC) is a rare sporadic or hereditary central nervous system (CNS) abnormality, characterized by symmetric or asymmetric calcification of the basal ganglia. CASE REPORT We report the case of a 65-year-old Gypsy female who was admitted for a tetanic seizure, and who had a history of polyneuropathy, restless-leg syndrome, retinopathy, diabetes, hyperlipidemia, osteoporosis with consecutive hyperkyphosis, cervicalgia, lumbalgia, struma nodosa requiring thyroidectomy and consecutive hypothyroidism, adipositas, resection of a vocal chord polyp, arterial hypertension, coronary heart disease, atheromatosis of the aorta, peripheral artery disease, chronic obstructive pulmonary disease, steatosis hepatis, mild renal insufficiency, long-term hypocalcemia, hyperphosphatemia, impingement syndrome, spondylarthrosis of the lumbar spine, and hysterectomy...
April 9, 2017: American Journal of Case Reports
https://www.readbyqxmd.com/read/28388575/cofilin-1-induces-the-epithelial-mesenchymal-transition-of-gastric-cancer-cells-by-promoting-cytoskeletal-rearrangement
#5
Wang Haibo, Tao Lide, Jin Feng, Gu Hao, Dai Xiaojun, Ni Tengyang, Feng Jun, Ding Yanbing, Xiao Weiming, Qian Yayun, Liu Yanqing
Epithelial-mesenchymal transition (EMT) is an important biological process whereby malignant tumor cells obtain the ability to migrate, invade, resist apoptosis and degrade the extracellular matrix. We found that Cofilin1 (CFL1) expression was elevated in clinical gastric cancer specimens and correlated with biomarkers of EMT in BGC-823 gastric cancer cells. BGC-823 cells exhibited EMT phenotypes and increased metastatic ability when induced by TGF-β1. By contrast, BGC-823 cells transfected with Lv-siRNA-CFL1 did not exhibit EMT phenotypes under the same inducing conditions...
March 27, 2017: Oncotarget
https://www.readbyqxmd.com/read/28388537/ndv-d90-suppresses-growth-of-gastric-cancer-and-cancer-related-vascularization
#6
Hong Sui, Kaibing Wang, Rui Xie, Xi Li, Kunpeng Li, Yuxian Bai, Xishan Wang, Bin Bai, Dan Chen, Jiazhuang Li, Baozhong Shen
Recent reports suggest promises on using oncolytic Newcastle disease viruses (NDV) to treat different cancers, while the effects of a NDV-D90 strain on gastric cancer remain unknown. Here we showed that NDV-D90 induced gastric cancer cell apoptosis in a dose-dependent manner in 3 gastric cancer cell lines BGC-823, SGC-7901 and MKN-28. Pronounced reduction in cell invasion was detected in NDV-D90-treated BGC-823 and SGC-7901 cells, but not in MKN-28 cells. The increases in cell apoptosis and reduction in cell growth in NDV-D90-treated gastric cancer cells seemingly resulted from augmentation of p38 signaling and suppression of ERK1/2 and Akt signaling...
March 25, 2017: Oncotarget
https://www.readbyqxmd.com/read/28374459/new-resorcinol-derivatives-from-a-sponge-derived-fungus-hansfordia-sinuosae
#7
Zehong Wu, Yuan Li, Dong Liu, Min Ma, Jianliang Chen, Wenhan Lin
Ten new resorcinol derivatives named Hansfordiols A-J (1-10) were isolated from the fermentation broth of the sponge-derived fungus Hansfordia sinuosae. High resolution electron ionization mass spectrometry, FT-IR spectroscopy, and NMR techniques were used to elucidate the structures of these compounds, and the absolute configurations were established by the modified Mosher's method and their specific optical rotation. The structures of compounds 8-10 were featured with various chlorination at aromatic rings...
April 4, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28373542/biosynthesis-of-the-pyrrolidine-protein-synthesis-inhibitor-anisomycin-involves-novel-gene-ensemble-and-cryptic-biosynthetic-steps
#8
Xiaoqing Zheng, Qiuxiang Cheng, Fen Yao, Xiaozheng Wang, Lingxin Kong, Bo Cao, Min Xu, Shuangjun Lin, Zixin Deng, Yit-Heng Chooi, Delin You
The protein synthesis inhibitor anisomycin features a unique benzylpyrrolidine system and exhibits diverse biological and pharmacologic activities. Its biosynthetic origin has remained obscure for more than 60 y, however. Here we report the identification of the biosynthetic gene cluster (BGC) of anisomycin in Streptomyces hygrospinosus var. beijingensis by a bioactivity-guided high-throughput screening method. Using a combination of bioinformatic analysis, reverse genetics, chemical analysis, and in vitro biochemical assays, we have identified a core four-gene ensemble responsible for the synthesis of the pyrrolidine system in anisomycin: aniQ, encoding a aminotransferase that catalyzes an initial deamination and a later reamination steps; aniP, encoding a transketolase implicated to bring together an glycolysis intermediate with 4-hydroxyphenylpyruvic acid to form the anisomycin molecular backbone; aniO, encoding a glycosyltransferase that catalyzes a cryptic glycosylation crucial for downstream enzyme processing; and aniN, encoding a bifunctional dehydrogenase that mediates multistep pyrrolidine formation...
April 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28360891/cloning-and-heterologous-expression-of-a-large-sized-natural-product-biosynthetic-gene-cluster-in-streptomyces-species
#9
REVIEW
Hee-Ju Nah, Hye-Rim Pyeon, Seung-Hoon Kang, Si-Sun Choi, Eung-Soo Kim
Actinomycetes family including Streptomyces species have been a major source for the discovery of novel natural products (NPs) in the last several decades thanks to their structural novelty, diversity and complexity. Moreover, recent genome mining approach has provided an attractive tool to screen potentially valuable NP biosynthetic gene clusters (BGCs) present in the actinomycetes genomes. Since many of these NP BGCs are silent or cryptic in the original actinomycetes, various techniques have been employed to activate these NP BGCs...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28356986/hypoxia-regulates-cd44-expression-via-hypoxia-inducible-factor-1%C3%AE-in-human-gastric-cancer-cells
#10
Gai Liang, Shuang Li, Wei Du, Qinghua Ke, Jun Cai, Jiyuan Yang
Hypoxia induces proliferation and invasion in cancer cells via hypoxia-inducible factor (HIF)-1α. The cell adhesion molecule cluster of differentiation (CD) 44 has been associated with increased cell invasion and metastasis. Whether hypoxia regulates the expression of CD44 in gastric cancer cells remains to be established. In the current study, the effects of hypoxia on HIF-1α and CD44 expression levels in human gastric cell lines SGC-7901 and BGC-823 were evaluated. The cells were cultured in 1% O2 for 1 week and then treated with 20 nM rapamycin for 72 h...
February 2017: Oncology Letters
https://www.readbyqxmd.com/read/28356958/bioinformatic-identification-of-candidate-genes-induced-by-trichostatin-a-in-bgc-823-gastric-cancer-cells
#11
Yunlong Li, Lisha Zhang, Chunfa Yang, Riheng Li, Longbin Shang, Xiaoming Zou
The aim of the present study was to identify the candidate genes induced by trichostatin A (TSA) in BGC-823 gastric cancer (GC) cells and to explore the possible inhibition mechanism of TSA in GC. Gene expression data were obtained through chip detection, and differentially expressed genes (DEGs) between GC cells treated with TSA and untreated GC cells (control group) were identified. Gene ontology analysis of the DEGs was performed using the database for annotation, visualization and integrated discovery. Then sub-pathway enrichment analysis was performed and a microRNA (miRNA) regulatory network was constructed...
February 2017: Oncology Letters
https://www.readbyqxmd.com/read/28347917/induction-of-apoptotic-dna-fragmentation-mediated-by-mitochondrial-pathway-with-caspase-3-dependent-bid-cleavage-in-human-gastric-cancer-cells-by-a-new-nitroxyl-spin-labeled-derivative-of-podophyllotoxin
#12
Yu-Jie Yang, She-Ning Qi, Rui-Yue Shi, Jun Yao, Li-Sheng Wang, Hu-Qin Yuan, Yuan-Xue Jing
PURPOSE: 4-[4''-(2'', 2'', 6'', 6''-tetramethyl-l''-piperidinyloxy) amino]-4'-demethyl-epipodophyllotoxin (GP7) is a new semi-synthesized nitroxyl spin-labeled derivative of podophyllotoxin with anti-leukemic and anti-osteosarcoma effects. The purpose of the present study is to investigate the anti-gastric cancer (GC) effects of GP7 and the possible involvement of caspase pathway in GP7-induced apoptotic DNA fragmentation in human GC cells. MATERIALS AND METHODS: Effects of GP7 on the proliferation of human GC cell lines MKN28, AGS, BGC-823 and HGC-27 in different degrees of differentiation and normal human gastric epithelial cell line GES-1 were studied by MTT assay and compared with the effects of etoposide...
March 24, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28344324/diallyl-trisulfide-suppresses-tumor-growth-through-the-attenuation-of-nrf2-akt-and-activation-of-p38-jnk-and-potentiates-cisplatin-efficacy-in-gastric-cancer-treatment
#13
Xiao-Yan Jiang, Xiao-Song Zhu, Hong-Ya Xu, Zhong-Xi Zhao, Si-Ying Li, Shan-Zhong Li, Jian-Hua Cai, Ji-Min Cao
Diallyl trisulfide (DATS), a garlic organosulfide, has shown excellent chemopreventive potential. Cisplatin (DDP) is widely used to treat solid malignant tumors, but causing serious side effects. In the current study, we attempted to elucidate the chemopreventive mechanisms of DATS in human gastric cancer BGC-823 cells in vitro, and to investigate whether DATS could enhance the anti-tumor efficacy of DDP and improve quality of life in BGC-823 xenograft mice in vivo. Treatment with DATS (25-400 μmol/L) dose-dependently inhibited the viability of BGC-823 cells in vitro with an IC50 of 115...
March 27, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28339092/macc1-decreases-the-chemosensitivity-of-gastric-cancer-cells-to-oxaliplatin-by-regulating-fasn-expression
#14
Jiangman Duan, Lishan Chen, Minyu Zhou, Jingwen Zhang, Li Sun, Na Huang, Jianping Bin, Yulin Liao, Wangjun Liao
The effect of chemotherapeutic agents is limited as a result of drug resistance, which demands prompt solutions provided by clinical studies. To date, the underlying mechanisms of chemotherapy resistance are relatively unknown. Metastasis-associated in colon cancer 1 (MACC1) is an oncogene associated with the progression and prognosis of gastric cancer (GC). Bioinformatic analysis revealed that MACC1 is positively associated with fatty acid synthase (FASN), a major enzyme of lipogenesis, and drives chemoresistance to oxaliplatin in GC...
March 22, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28337309/gkn2-increases-apoptosis-reduces-the-proliferation-and-invasion-ability-of-gastric-cancer-cells-through-down-regulating-the-jak-stat-signaling-pathway
#15
Jun Ouyang, Xiaohui Pan, Hui Lin, Zecheng Hu, Ping Xiao, Haobin Hu
OBJECTIVES: To investigate the effect of gastric motility protein 2 (GKN2) on the proliferation, apoptosis and invasion of gastric cancer cell and on the JAK/signal transducer and activator of transcription 3 (STAT3) signaling pathway. METHODS: Expression of GKN2 was qualified using Western blot analysis in four gastric cancer cell lines and immortalized human gastric mucosal epithelial cell line GES-1. The cells were then transfected with pcDNA3.1-GKN2 and control vector using Lipofectamine(tm)2000 and assayed for viability, apoptosis, cell cycle changes, invasion ability as well as expression of cell cycle protein D1 (Cylin D1), Bcl-2, Bax, matrix metalloproteinase 2 (MMP2), MMP9, JAK2 and p-STAT3...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28322864/water-soluble-derivatives-of-25-och3-ppd-and-their-anti-proliferative-activities
#16
Wu-Xi Zhou, Yuan-Yuan Sun, Wei-Hui Yuan, Yu-Qing Zhao
(20R)-25-Methoxyl-dammarane-3β,12β,20-triol (25-OCH3-PPD, AD-1) is a dammarane-type sapogenin showing anti-tumor potential. In the search for new anti-tumor agents with higher potency than our previously identified compound 25-OCH3-PPD, 11 novel sulfamic acid and diacid derivatives that could improve water solubility and contribute to good drug potency and pharmacokinetic profiles were designed and synthesized. Their in vitro anti-tumor activities in MCF-7, A-549, HCT-116, and BGC-823 cell lines and one normal cell line were tested by standard MTT assay...
March 18, 2017: Steroids
https://www.readbyqxmd.com/read/28281844/cxcr7-as-a-chemokine-receptor-for-sdf-1-promotes-gastric-cancer-progression-via-mapk-pathways
#17
Ameng Shi, Haitao Shi, Lei Dong, Shaoxian Xu, Miao Jia, Xiaoyan Guo, Ting Wang
OBJECTIVE: As the alternate receptor for stromal cell-derived factor-1 (SDF-1) except CXCR4, CXCR7 has been shown to be involved in the progression of some malignancies. However, the role of SDF-1/CXCR7 in gastric cancer (GC) remains unclear. MATERIALS AND METHODS: CXCR7 expression was examined in 83 human GC tissues and adjacent non-cancer tissues (ANCTs) by immunohistochemistry, in three human GC cell lines (MGC-803, BGC-823 and SGC-7901) by reverse transcription-PCR and western blot...
June 2017: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/28276770/synthesis-and-cytotoxic-activity-of-two-steroids-icogenin-aglycone-analogs
#18
Yu-Yao Guan, Shu-Zhen Li, Ping-Sheng Lei
During the process of icogenin analog research, we obtained two cytotoxic steroids: compound 4 and compound 6 casually. Their in vitro antitumor activities were tested by the standard MTT assay. The results disclosed that compound 4 (IC50 = 3.65-6.90 μM) showed potential antitumor activities against HELA, KB cell lines and compound 6 (IC50 = 2.40-9.05 μM) showed potential antitumor activities against HELA, BGC-823, KB, A549, HCT-8 cell lines.
September 22, 2016: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/28264235/efficacy-of-balloon-guiding-catheter-for-mechanical-thrombectomy-in-patients-with-anterior-circulation-ischemic-stroke
#19
Jae-Sang Oh, Seok-Mann Yoon, Jai-Joon Shim, Jae-Won Doh, Hack-Gun Bae, Kyeong-Seok Lee
OBJECTIVE: To evaluate the efficacy of balloon guiding catheter (BGC) during thrombectomy in anterior circulation ischemic stroke. METHODS: Sixty-two patients with acute anterior circulation ischemic stroke were treated with thrombectomy using a Solitaire stent from 2011 to 2016. Patients were divided into the BGC group (n=24, 39%) and the non-BGC group (n=38, 61%). The number of retrievals, procedure time, thrombolysis in cerebral infarction (TICI) grade, presence of distal emboli, and clinical outcomes at 3 months were evaluated...
March 2017: Journal of Korean Neurosurgical Society
https://www.readbyqxmd.com/read/28261170/the-global-regulators-lrp-leuo-and-hexa-control-secondary-metabolism-in-entomopathogenic-bacteria
#20
Yvonne Engel, Carina Windhorst, Xiaojun Lu, Heidi Goodrich-Blair, Helge B Bode
Photorhabdus luminescens TTO1 and Xenorhabdus nematophila HGB081 are insect pathogenic bacteria and producers of various structurally diverse bioactive natural products. In these entomopathogenic bacteria we investigated the role of the global regulators Lrp, LeuO, and HexA in the production of natural products. Lrp is a general activator of natural product biosynthesis in X. nematophila and for most compounds in TTO1. Microarray analysis confirmed these results in X. nematophila and enabled the identification of additional biosynthesis gene clusters (BGC) regulated by Lrp...
2017: Frontiers in Microbiology
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