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Li-Ping Dai, Ling-Xia Zhang, Ya-Lin Liu, Hong Wu, Rui-Xin Liu, Meng Zhao, Shuang-Shuang Tian, Xue Jiang, Sui-Qing Chen
Cytotoxic diterpenoids were enriched and orientation prepared from the aerial parts of Isodon excisoides target-guided by UPLC-LTQ-Orbitrap-MS. Four diterpenoids were obtained, including a novel compound: 1α-acetoxy-7α, 14β, 20α-trihydroxy-ent-kaur-16-en-15-one (1); together with three known compounds kamebakaurin (2), lasiokaurin (3), enmenol-1-β-glucoside (4). Their structures were elucidated on the basis of spectroscopic methods in conjunction with published data for their analogues. All compounds were tested for their cytotoxic effects against five human cancer cell lines HCT-116, HepG2, A2780, NCI-H1650 and BGC-823, respectively...
January 10, 2018: Natural Product Research
Cheng-Jung Sung, Katherine L Bell, Chris C Nice, Noland H Martin
Hybrid zones provide unique opportunities to examine reproductive isolation and introgression in nature. We utilized 45,384 Single Nucleotide Polymorphism (SNP) loci to perform association mapping of 14 floral, vegetative, and ecological traits that differ between Iris hexagona and Iris fulva, and to investigate, using a Bayesian Genomic Cline (BGC) framework, patterns of genomic introgression in a large and phenotypically diverse hybrid zone in southern Louisiana. Many loci of small effect-size were consistently found to be associated with phenotypic variation across all traits, and several individual loci were revealed to influence phenotypic variation across multiple traits...
January 10, 2018: Molecular Ecology
Jin-Feng Wang, Ying Wang, Hong-Liang Wang, Cheng-Bi Xu, Hai-Tao Wang
A chemical investigation on the 70% ethanol extract from the leaves and stems of Kopsia lancibracteolata Merr. resulted in the isolation of three new methyl chanofruticosinate type alkaloids, 12-hydroxyl prunifoline A (1), 12-hydroxyl prunifoline C (2), and N(4)-oxide prunifoline D (3). Structural elucidation of all the compounds was performed by spectral methods such as 1D- and 2D-NMR, IR, UV, and HR-ESI-MS. The isolated alkaloids were tested in vitro for cytotoxic potential against five tumor cell lines (BGC-823, HepG2, MCF-7, SGC-7901, and SK-MEL-2)...
January 3, 2018: Journal of Asian Natural Products Research
Jiabing Wang, Di Yun, Jiali Yao, Weitao Fu, Fangyan Huang, Liping Chen, Tao Wei, Cuijuan Yu, Haineng Xu, Xiaoou Zhou, Yanqing Huang, Jianzhang Wu, Peihong Qiu, Wulan Li
Molecular hybridization is considered as an effective tactic to develop drugs for the treatment of cancer. A series of novel hybrid compounds of isatin and Michael acceptor were designed and synthesized on the basis of association principle. These hybrid compounds were tested for cytotoxic potential against human cancer cell lines namely, BGC-823, SGC-7901 and NCI-H460 by MTT assay. Most compounds showed good anti-growth activities in all tested human cancer cells. SAR and QSAR analysis may provide vital information for the future development of novel anti-cancer inhibitors...
December 18, 2017: European Journal of Medicinal Chemistry
Wei Wei, Li Shi, Weichang Chen, Liyu Hu, Dan Chen, Xiaoying Shi, Hua Xiang, Cunguo Guo, Zhenping Wu
This study aimed to investigate the regulatory effects of microRNA (miR)‑200c on the proliferation, apoptosis and invasion of gastric carcinoma cells and to elucidate the underlying mechanisms involving the possible role of endothelin receptor A (EDNRA). The expression levels of miR‑200c and EDNRA in the gastric carcinoma cell lines, BGC‑823, SGC‑7901 and HGC‑27, and in GES‑1 normal gastric cells were evaluated by RT‑PCR and western blot analysis. The gastric carcinoma cells, particularly the BGC‑823 cells, expressed significantly lower levels of miR‑200c than the normal gastric cells (P<0...
December 12, 2017: International Journal of Molecular Medicine
Weili Chen, Juan Li, Honghui Zhu, Pengyang Xu, Jiezhong Chen, Qing Yao
Arbuscular mycorrhizal fungi (AMF) establish symbiosis with most terrestrial plants, and greatly regulate lateral root (LR) formation. Phosphorus (P), sugar, and plant hormones are proposed being involved in this regulation, however, no global evidence regarding these factors is available so far, especially in woody plants. In this study, we inoculated trifoliate orange seedlings (Poncirus trifoliata L. Raf) with an AMF isolate, Rhizophagus irregularis BGC JX04B. After 4 months of growth, LR formation was characterized, and sugar contents in roots were determined...
2017: Frontiers in Plant Science
Tomotaka Ohshima, Chinmaya Dash, Andrey Belayev, Taiki Yamamoto, Shunsaku Goto, Yoko Kato
The use of 8-F balloon guide catheter (BGC) for proximal flow control was previously shown to prevent distal embolic complications during mechanical clot retrieval in patients with acute ischemic stroke. In this retrospective study, the utility of 8-F BGCs for proximal flow control during endovascular coiling of anterior circulation aneurysms was investigated. Patients who underwent endovascular coiling for anterior circulation aneurysms between August 2013 and December 2016 were retrospectively analyzed. Among a total of 152 patients included in this series, 64 patients presented with aneurysmal rupture, whereas the aneurysms were detected incidentally or due to mass effects in the remaining patients...
November 2017: Nagoya Journal of Medical Science
Laiqin Tang, Zhiguo Long, Guangjia Feng, Xianzhi Guo, Minghua Yu
Trastuzumab, a humanized antibody targeting human epidermal growth factor receptor 2 (HER2), exhibits remarkable therapeutic efficacy against HER2-positive gastric cancer. Acquired resistance to trastuzumab remains a barrier to patient survival and the mechanisms underlying this are still not well understood. The normal epithelial cell-specific-1 (NES1) gene, also named as KLK10, is recognized as a potential therapeutic target for reversing trastuzumab resistance. The aim of this study was to explore the potential role of KLK10 in trastuzumab resistance (TR) gastric cancer cells...
December 12, 2017: Journal of Cellular Biochemistry
Gregory C A Amos, Takayoshi Awakawa, Robert N Tuttle, Anne-Catrin Letzel, Min Cheol Kim, Yuta Kudo, William Fenical, Bradley S Moore, Paul R Jensen
Bacterial natural products remain an important source of new medicines. DNA sequencing has revealed that a majority of natural product biosynthetic gene clusters (BGCs) maintained in bacterial genomes have yet to be linked to the small molecules whose biosynthesis they encode. Efforts to discover the products of these orphan BGCs are driving the development of genome mining techniques based on the premise that many are transcriptionally silent during normal laboratory cultivation. Here, we employ comparative transcriptomics to assess BGC expression among four closely related strains of marine bacteria belonging to the genus Salinispora The results reveal that slightly more than half of the BGCs are expressed at levels that should facilitate product detection...
December 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
Chengyun Li, Geyu Liang, Sheng Yang, Jing Sui, Wenzhuo Yao, Xian Shen, Yanqiu Zhang, Hui Peng, Weiwei Hong, Siyi Xu, Wenjuan Wu, Yancheng Ye, Zhiyi Zhang, Wenhua Zhang, Lihong Yin, Yuepu Pu
The long non-coding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) has been recently shown to be dysregulated during disease occurrence and to play an important role in the progression of several cancers. However, the biological role and potential regulation mechanism of UCA1 in the carcinogenesis of gastric cancer remain unclear. In the present study, we found that UCA1 was aberrantly upregulated in gastric cancer tissues and gastric cancer cell lines, and was associated with TNM stage and metastasis...
November 7, 2017: Oncotarget
S Dinesh Kumar, K Mohamed Abudhahir, N Selvamurugan, S Vimalraj, R Murugesan, N Srinivasan, A Moorthi
The improvisation of the treatment procedures for treating the various kind of bone defects such as, bone or dental trauma and for diseases such as osteoporosis, osteomyelitis etc., need the suitable and promising biomaterials with resemblance of bone components. Bioactive glass ceramic (BGC) has recently acquired great attention as the most promising biomaterials; hence it has been widely applied as a filler material for bone tissue regeneration. Because it elicts specific biological responses after implantation in addition more potential in formation of strong interface with both hard and soft tissues by dissolution of calcium and phosphate ions...
February 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Xian Xu, Guo-Qin Zhu, Kai Zhang, Yi-Chan Zhou, Xiao-Lin Li, Wei Xu, Hao Zhang, Yun Shao, Zhen-Yu Zhang, Wei-Hao Sun
Ursolic acid (UA) induces apoptosis in gastric cancer cells by inhibiting cyclooxygenase-2 (COX-2). Paclitaxel (PTX) is an important chemotherapy agent used to treat solid tumors. We evaluated the in vitro antitumor activity of UA in combination with PTX against gastric cancer cells and investigated the mechanisms underlying the combined effects. A cytotoxicity test and flow cytometry were utilized to study the effects of UA and PTX on proliferation and apoptosis, respectively. To further elucidate the mechanism, Western blot analysis was used to assess changes in the expression of a series of related proteins, including COX-2, proliferating cell nuclear antigen (PCNA), Bcl-2, and Bax...
November 3, 2017: Oncotarget
Kirk A Reynolds, Hanna Luhavaya, Jie Li, Samira Dahesh, Victor Nizet, Kazuya Yamanaka, Bradley S Moore
In the ongoing effort to unlock the chemical potential of marine bacteria, genetic engineering of biosynthetic gene clusters (BGCs) is increasingly used to awake or improve expression of biosynthetic genes that may lead to discovery of novel bioactive natural products. Previously, we reported the successful capture, engineering and heterologous expression of an orphan BGC from the marine actinomycete Saccharomonospora sp. CNQ-490, which resulted in the isolation of the novel lipopeptide antibiotic taromycin A...
November 29, 2017: Journal of Antibiotics
Saima Khalid, Joshua A Baccile, Joseph E Spraker, Joanna Tannous, Muhammad Imran, Frank C Schroeder, Nancy P Keller
Bacterial-fungal interactions are presumed to be mediated chiefly by small-molecule signals; however, little is known about the signaling networks that regulate antagonistic relationships between pathogens. Here we show that the ralstonins, lipopeptides produced by the plant pathogenic bacteria Ralstonia solanacearum, interfere with germination of the plant-pathogenic fungus Aspergillus flavus by down-regulating expression of a cryptic biosynthetic gene cluster (BGC), named imq. Comparative metabolomic analysis of overexpression strains of the transcription factor ImqK revealed imq-dependent production of a family of tripeptide-derived alkaloids, the imizoquins...
November 28, 2017: ACS Chemical Biology
Shanshan Wang, Haibo Han, Ying Hu, Wei Yang, Yunwei Lv, Limin Wang, Lianhai Zhang, Jiafu Ji
Solute carrier family 3 member 2 (SLC3A2) has been reported to be highly expressed in a variety of carcinomas. However, the function of SLC3A2 in gastric carcinoma (GC) has not been well explored. Monoclonal antibody (mAb) 3G9, generated from immunogen of various human GC cell lines, has been shown to bind to GC tissues specifically. In this study, we identified the target antigen of mAb 3G9 as SLC3A2, and detected the expression profile of SLC3A2 in a panel of gastric cancer cell lines and GC tumor tissues...
October 24, 2017: Oncotarget
Fuhao Qiao, Jian Fang, Jinfeng Xu, Wenqiu Zhao, Ying Ni, Bufugdi Andreas Akuo, Wei Zhang, Yun Liu, Fangfang Ding, Guanlin Li, Baoguo Liu, Hua Wang, Shihe Shao
Adrenomedullin has been shown to be overexpressed in many tumors, including gastric cancer tumors; however, its mechanism of action remains unclear. In this study, we examined the role of adrenomedullin in the pathogenesis of gastric cancer. Using clinical specimens and immunohistochemistry, we found that the expression levels of adrenomedullin and its receptors are inordinately elevated as compared to the adjacent non-tumor gastric tissues. We used siRNA gene silencing, in BGC-823 gastric cancer cell lines, to target adrenomedullin genes, and found that increased adrenomedullin expression results in the proliferation of tumor cells, tumor invasion, and metastasis...
October 24, 2017: Oncotarget
Hong Zhang, Ruwei Wang, Huaqin Xue, Ruoyu Hu, Guijian Liu
The characteristics of atmospheric PM10- and PM2.5-bound polycyclic aromatic hydrocarbons (PAHs) were investigated in Tongling city, China. Results showed that the total concentrations of PM10- and PM2.5-bound PAHs exhibited distinct seasonal and spatial variability. The metallurgic sites showed the highest PAH concentrations, which is mainly attributed to the metallurgic activities (mainly copper ore smelting) and coal combustion as the smelting fuel. The rural area showed the lowest concentrations, but exhibited significant increase from summer to autumn...
November 25, 2017: Bulletin of Environmental Contamination and Toxicology
Ruth Gostelow, Katarina Hazuchova, Christopher Scudder, Yaiza Forcada, David Church, Stijn J M Niessen
Objectives The objective was to evaluate a nadir-led protocol for transitioning porcine lente insulin suspension (PLIS)-treated diabetic cats onto human recombinant protamine zinc insulin (PZIR). Methods Recently diagnosed (<5 months) diabetic cats, treated with PLIS q12h for ⩾6 weeks, were recruited. Fructosamine, 24 h blood glucose curve (BGC), quality of life assessment (DIAQoL-pet score) and Diabetic Clinical Score (DCS) were assessed at enrolment (PLIS-treated) and 2, 4 and 12 weeks after transitioning to PZIR (starting dose 0...
March 1, 2017: Journal of Feline Medicine and Surgery
L L Bolotskaya, E G Bessmertnaya, M V Shestakova, M Sh Shamkhalova, L V Nikankina, A V Ilyin, I S Glek, A V Zolotukhin, I I Dedov
AIM: To assess the time course of changes in the level of glycated hemoglobin (HbA1c) for 20 years after the onset of type 1 diabetes mellitus (T1DM) and to compare its correlation with the development of microvascular complications, such as diabetic retinopathy (DR) and diabetic nephropathy (DN). SUBJECTS AND METHODS: A total of 187 children with new-onset T1DM were registered in Moscow in 1994. During the 20-year follow-up study, these patients underwent regular check-ups at the Endocrinology Research Center, Ministry of Health of the Russian Federation, which included assessment of physical data, HbA1c 2-4 times a year, biochemical blood and albuminuria tests (once per year), and ophthalmologic examination (twice a year)...
2017: Terapevticheskiĭ Arkhiv
Wei Zhou, Xueqiong Fu, Honggang Yu
Objective To investigate the effect of lentivirus-mediated shRNA targeting AKT1 gene on the sensitivity of gastric cancer xenografts to doxorubicin. Methods To establish the lentiviral vector of AKT1 RNA interference (RNAi), the vector of pGCSIL-shAKT1-GFP was infected into HEK293T cells, and meanwhile, the empty vector pGCSIL-shCON-GFP was assigned as a blank group, and then the viruses were harvested. BGC-823 gastric cancer cells were infected with the viruses. The protein expression of AKT1 was detected by Western blotting...
October 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
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