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https://www.readbyqxmd.com/read/28100567/mecp2-regulated-glycogenes-contribute-to-proliferation-and-apoptosis-of-gastric-cancer-cells
#1
Yannan Qin, Lingyu Zhao, Xiaofei Wang, Dongdong Tong, Christopher Hoover, Fei Wu, Yingxun Liu, Lumin Wang, Liying Liu, Lei Ni, Tusheng Song, Chen Huang
Aberrant glycogene and glycan expression is intimately associated with carcinogenesis, invasion, and metastasis of gastric cancer (GC); however the regulatory mechanisms for glycogenes in GC cells remain unclear. Methyl-CpG-binding protein 2 (MeCP2) regulates genes by binding to methylated promoters, and in our previous work we found that it is overexpressed in GC cell lines and tissues, functioning as an oncogene. In this study we detected the expression of 212 glycogenes in MeCP2 silenced GC cells versus control using the Agilent Whole Human Genome Microarray and mining the data through bioinformatic analysis...
January 18, 2017: Glycobiology
https://www.readbyqxmd.com/read/28099141/antitumor-activity-of-a-trans-thiosemicarbazone-schiff-base-palladium-ii-complex-on-human-gastric-adenocarcinoma-cells
#2
Bingchang Zhang, Haiqing Luo, Qinjuan Xu, Lirong Lin, Bing Zhang
The development of transition-metal-based antitumor drug candidates increases the metallopharmaceuticals study dramatically. Two trans-thiosemicarbazone-based, Schiff base palladium (Pd) (II) complexes, DMABTSPd (TSPd) and DMABPTSPd (PTSPd), were prepared and characterized as described in our previous study. Here, we investigated whether the two complexes have antitumor effect on human gastric adenocarcinoma cell lines, BGC-823 and SGC-7901, compared with normal human gastric mucosal epithelial cell line, Ges-1...
January 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/28092748/optimizing-selective-cutting-strategies-for-maximum-carbon-stocks-and-yield-of-moso-bamboo-forest-using-biome-bgc-model
#3
Fangjie Mao, Guomo Zhou, Pingheng Li, Huaqiang Du, Xiaojun Xu, Yongjun Shi, Lufeng Mo, Yufeng Zhou, Guoqing Tu
The selective cutting method currently used in Moso bamboo forests has resulted in a reduction of stand productivity and carbon sequestration capacity. Given the time and labor expense involved in addressing this problem manually, simulation using an ecosystem model is the most suitable approach. The BIOME-BGC model was improved to suit managed Moso bamboo forests, which was adapted to include age structure, specific ecological processes and management measures of Moso bamboo forest. A field selective cutting experiment was done in nine plots with three cutting intensities (high-intensity, moderate-intensity and low-intensity) during 2010-2013, and biomass of these plots was measured for model validation...
January 13, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28088540/multiplexed-site-specific-genome-engineering-for-overproducing-bioactive-secondary-metabolites-in-actinomycetes
#4
Lei Li, Guosong Zheng, Jun Chen, Mei Ge, Weihong Jiang, Yinhua Lu
Actinomycetes produce a large variety of pharmaceutically active compounds, yet production titers often require to be improved for discovery, development and large-scale manufacturing. Here, we describe a new technique, multiplexed site-specific genome engineering (MSGE) via the 'one integrase-multiple attB sites' concept, for the stable integration of secondary metabolite biosynthetic gene clusters (BGCs). Using MSGE, we achieved five-copy chromosomal integration of the pristinamycin II (PII) BGC in Streptomyces pristinaespiralis, resulting in the highest reported PII titers in flask and batch fermentations (2...
January 11, 2017: Metabolic Engineering
https://www.readbyqxmd.com/read/28073676/sulfamic-and-succinic-acid-derivatives-of-25-oh-ppd-and-their-activities-to-mcf-7-a-549-hct-116-and-bgc-823-cell-lines
#5
Wu-Xi Zhou, Jia-Qing Cao, Xu-De Wang, Jun-Hui Guo, Yu-Qing Zhao
In the search for new anti-tumor agents with higher potency than our previously identified compound 1 (25-OH-PPD, 25-hydroxyprotopanaxadiol), 12 novel sulfamic and succinic acid derivatives that could improve water solubility and contribute to good drug potency and pharmacokinetic profiles were designed and synthesized. Their in vitro anti-tumor activities in MCF-7, A-549, HCT-116, and BGC-823 cell lines and one normal cell line were tested by standard MTT assay. Results showed that compared with compound 1, compounds 2, 3, and 7 exhibited higher cytotoxic activity on A-549 and BGC-823 cell lines, together with lower toxicity in the normal cell...
December 21, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28034823/structural-physicochemical-antioxidant-and-antitumor-property-of-an-acidic-polysaccharide-from-polygonum-multiflorum
#6
Weili Zhu, Xiaoping Xue, Zhanjun Zhang
In this study, the structural characterization, physicochemical property, antioxidant and antitumor activity of an acidic polysaccharide (APS) from Polygonum multiflorum were investigated. Monosaccharide composition analysis showed APS was composed of arabinose, rhamnose, galactose and galacturonic acid in the molar ratio of 1.23:1.32:1.48:1.00. The presence of uronic acid was also confirmed by the bands at 1740, 1645 and 1425cm(-1) on Fourier transform-infrared spectroscopy. Methylation and nuclear magnetic resonance analyses showed APS was mainly composed by the residues of →5)-α-l-Araf-(1→, →3)-β-d-Galp-(1→, →3,6)-β-d-Galp-(1→, →4)-α-d-GalAp-(1→ and →2)-α-l-Rhap-(1→ in the backbone...
December 26, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27998431/-expression-of-long-noncoding-rna-stcat3-in-gastric-cancer-tissues-and-its-effect-on-malignant-phenotype-of-gastric-cancer-cells
#7
J F Zhang, Z S Sun, Q F Zhang, W F Ding, X H Wu, Z B Mao
Objective: To detect the expression of long noncoding RNA(lncRNA)stomach cancer-associated transcript-3(STCAT3) in gastric cancer tissues, adjacent tissues, human gastric cancer cell lines and normal gastric epithelial cell lines, and to investigate the relationship between STCAT3 expression and clinicopathological features and malignant phenotype of gastric cancer. Methods: Quantitative fluorescent real-time polymerase chain reaction (qRT-PCR) was applied to detect the lncRNA STCAT3 expression levels in gastric cancer tissues, paired adjacent non-tumorous tissues, in order to explore the relationship between STCAT3 expression and clinicopathological features of gastric cancer...
December 13, 2016: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/27994509/mir-99a-and-mir-491-regulate-cisplatin-resistance-in-human-gastric-cancer-cells-by-targeting-capns1
#8
Yajie Zhang, Wenxia Xu, Pan Ni, Aiping Li, Jianwei Zhou, Shan Xu
Cisplatin is the first-line agent utilized for the clinical treatment of a wide variety of solid tumors including gastric cancer. However, the intrinsic or acquired cisplatin resistance is often occurred in patients with gastric cancer and resulted in failure of cisplatin therapy. In order to investigate if miRNA involves in cisplatin resistance of human gastric cancer, we first screened and compared the expression of miRNAs between cisplatin resistant gastric cancer cell lines SGC-7901/DDP and BGC-823/DDP and their sensitive parental cells by miRNAs microarray and followed by analysis of 2D-GE/MS to identify their target proteins...
2016: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/27983921/long-non-coding-rna-taurine-upregulated-gene-1-promotes-cell-proliferation-and-invasion-in-gastric-cancer-via-negatively-modulating-mirna-145-5p
#9
Kewei Ren, Zhen Li, Yahua Li, Wenzhe Zhang, Xinwei Han
LncRNA taurine-upregulated gene 1 (TUG1) is involved in the development and carcinogenesis of various tumors, suggesting the diagnostic potential of TUG1 in these cancers. However, the exact role of TUG1 and its underlying mechanism in gastric cancer (GC) remain unknown. In this study, the expression of TUG1 and miR-145-5p in GC cell lines and non-malignant gastric epithelial cell line were detected by qRT-PCR. BGC-823 and SGC-7901 cells were transfected with si-TUG1, pcDNA 3.1-TUG1, miR-145-5p mimics, or matched controls...
8, 2016: Oncology Research
https://www.readbyqxmd.com/read/27917658/cytotoxic-coumaronochromones-from-the-inflorescences-of-celosia-cristata
#10
He Yan, Gonghai Zhang
Investigation on the MeOH extracts of the inflorescences of Celosia cristata led to the isolation of two new coumaronochromones, cristatone I (1) and cristatone II (2), along with three known flavones (3-5). Their structures were elucidated on the basis of spectroscopic analyses. Compounds 1-5 were tested for their cytotoxic activity against HeLa and BGC-823 cancer cell lines, of which cristatone II (2) showed interesting activity with the IC50 value of 23.82 μM.
December 5, 2016: Natural Product Research
https://www.readbyqxmd.com/read/27904131/mir-31-regulates-rho-associated-kinase-myosin-light-chain-rock-mlc-pathway-and-inhibits-gastric-cancer-invasion-roles-of-rhoa
#11
Zhuo Chen, Shengnan Liu, Yuan Xia, Kejian Wu
BACKGROUND This study evaluated how the expression of miR-31 can be used to detect gastric cancer (GC) to help illuminate the role of miR-31 and RhoA in GC cells. MATERIAL AND METHODS We carried out our experiments using tissue specimens from 70 GC patients. The relative expression of miR-31 and RhoA mRNA in tissues and cells was detected by RT-PCR. The expression level of RhoA protein was detected by immunohistochemistry. GC cell line BGC-823 was transfected with six groups of vectors: blank group, NC (negative control) group, miR-31 mimics group, miR-31 mimics + RhoA group, miR-31 mimics + ROCK group, and miR-31 mimics + MLCK agonist group...
December 1, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27898345/quercetin-loading-cdse-zns-nanoparticles-as-efficient-antibacterial-and-anticancer-materials
#12
Xiaofang Yang, Weiwei Zhang, Zhiwei Zhao, Nuan Li, Zhipeng Mou, Dongdong Sun, Yongping Cai, Weiyun Wang, Yi Lin
Quercetin (Qe) plays an important role in inflammation, antibacterial, anticancer, and aging. However, Qe has extremely low water solubility, which is a major challenge in drug absorption. In this study, we described a simple method for synthesis of Qe/CdSe/ZnS nanoparticles (QCZ NPs). The QCZ NPs had an average diameter of 10nm and prominent yellow emission under UV irradiation. We investigated the antibacterial activity of QCZ NPs against drug-resistant Escherichia coli (E. coli) and Bacillus subtilis (B...
February 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/27875306/family-wide-structural-characterization-and-genomic-comparisons-decode-the-diversity-oriented-biosynthesis-of-thalassospiramides-by-marine-proteobacteria
#13
Weipeng Zhang, Liang Lu, Qiliang Lai, Beika Zhu, Zhongrui Li, Ying Xu, Zongze Shao, Karl Herrup, Bradley S Moore, Avena C Ross, Pei-Yuan Qian
The thalassospiramide lipopeptides have great potential for therapeutic applications; however, their structural and functional diversity and biosynthesis are poorly understood. Here, by cultivating 130 Rhodospirillaceae strains sampled from oceans worldwide, we discovered 21 new thalassospiramide analogues and demonstrated their neuroprotective effects. To investigate the diversity of biosynthetic gene cluster (BGC) architectures, we sequenced the draft genomes of 28 Rhodospirillaceae strains. Our family-wide genomic analysis revealed three types of dysfunctional BGCs and four functional BGCs whose architectures correspond to four production patterns...
December 30, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27871039/synthesis-and-antitumor-activity-of-novel-substituted-uracil-1-n-acetic-acid-ester-derivatives-of-20-s-camptothecins
#14
Di-Zao Li, Qiang-Zhe Zhang, Cun-Ying Wang, Yan-Ling Zhang, Xing-Yu Li, Ji-Tao Huang, Hong-Yan Liu, Zhao-Di Fu, Hua-Xian Song, Jin-Ping Lin, Teng-Fei Ji, Xian-Dao Pan
A series of novel substituted uracil-1'(N)-acetic acid esters (6-20) of camptothecins (CPTs) were synthesized by the acylation method. These new compounds were evaluated for in vitro antitumor activity against tumor cell lines, A549, Bel7402, BGC-823, HCT-8 and A2780. In vitro results showed that most of the derivatives exhibited comparable or superior cytotoxicity compare to CPT (1) and topotecan (TPT, 2), with 12 and 13 possessing the best efficacy. Four compounds, 9, 12, 13 and 16, were selected to be evaluated for in vivo antitumor activity against H22, BGC-823 and Bel-7402 in mice...
January 5, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27835600/yap1-enhances-cell-proliferation-migration-and-invasion-of-gastric-cancer-in-vitro-and-in-vivo
#15
Dan Sun, Xiaoting Li, Yingjian He, Wenhui Li, Ying Wang, Huan Wang, Shanshan Jiang, Yan Xin
Yes-associated protein 1 (YAP1) plays an important role in the development of carcinomas such as breast, colorectal, and gastric (GC) cancers, but the role of YAP1 in GC has not been investigated comprehensively. The present study strongly suggests that YAP1 and P62 were significantly up-regulated in GC specimens, compared with normal gastric mucosa. In addition, the YAP1high P62high expression was independently associated with poor prognosis in GC (hazard ratio: 1.334, 95% confidence interval: 1.045-1.704, P = 0...
November 7, 2016: Oncotarget
https://www.readbyqxmd.com/read/27780355/development-of-visible-wavelength-maldi-cell-mass-spectrometry-for-high-efficiency-single-cell-analysis
#16
Caiqiao Xiong, Xiaoyu Zhou, Qing He, Xi Huang, Jiyun Wang, Wen-Ping Peng, Huan-Cheng Chang, Zongxiu Nie
Mass is a fundamental physical property of an individual cell, from which is revealed the cell growth, cycle, and activity. Taking advantage of cell mass spectrometry (CMS), accurate mass measurement of a charged single cell has been achieved. However, with the increasing demand for high-efficiency single-cell analysis in biology, the limited throughput and inefficient cell desorption/ionization of the CMS inevitably become important issues. To address the challenge, a state of the art visible-wavelength matrix assisted laser desorption/ionization (MALDI) CMS was developed...
December 6, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27769031/synthesis-sar-and-pharmacological-characterization-of-novel-anthraquinone-cation-compounds-as-potential-anticancer-agents
#17
Yanyan Zheng, Li Zhu, Lulu Fan, Wenna Zhao, Jianlong Wang, Xianxiao Hao, Yunhui Zhu, Xiufang Hu, Yaofeng Yuan, Jingwei Shao, Wenfeng Wang
Emodin, a natural anthraquinone derivative isolated from Rheum palmatum L., has been demonstrated to exhibit good anti-cancer effect. In this study, a series of novel quaternary ammonium salts of emodin, anthraquinone and anthrone were synthesized and their anticancer activities were tested in vitro. The effects of emodin quaternary ammonium salts on cell viability, apoptosis, intracellular ROS, and mitochondrial membrane potential were investigated in A375, BGC-823, HepG2 and HELF cells. The results demonstrated that compound 4a induced morphological changes and decreased cell viability...
January 5, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/27764763/lifestyle-and-dna-base-composition-in-polychaetes
#18
Andrea Tarallo, Maria Cristina Gambi, Giuseppe D'Onofrio
A comparative analysis of polychaete species, classified as motile and low-motile forms, highlighted that the former were characterized not only by a higher metabolic rate (MR), but also by a higher genomic GC content. The fluctuation of both variables was not affected by the phylogenetic relationship of the species. Thus, present results further support that a very active lifestyle affects MR and GC at the same time, showing an unexpected similarity between invertebrates and vertebrates. In teleosts, indeed, a similar pattern has been also observed in comparisons of migratory and nonmigratory species...
December 1, 2016: Physiological Genomics
https://www.readbyqxmd.com/read/27755746/the-effects-of-climate-downscaling-technique-and-observational-data-set-on-modeled-ecological-responses
#19
Afshin Pourmokhtarian, Charles T Driscoll, John L Campbell, Katharine Hayhoe, Anne M K Stoner
Assessments of future climate change impacts on ecosystems typically rely on multiple climate model projections, but often utilize only one downscaling approach trained on one set of observations. Here, we explore the extent to which modeled biogeochemical responses to changing climate are affected by the selection of the climate downscaling method and training observations used at the montane landscape of the Hubbard Brook Experimental Forest, New Hampshire, USA. We evaluated three downscaling methods: the delta method (or the change factor method), monthly quantile mapping (Bias Correction-Spatial Disaggregation, or BCSD), and daily quantile regression (Asynchronous Regional Regression Model, or ARRM)...
July 2016: Ecological Applications: a Publication of the Ecological Society of America
https://www.readbyqxmd.com/read/27716367/sdf-1-cxcr7-axis-regulates-the-proliferation-invasion-adhesion-and-angiogenesis-of-gastric-cancer-cells
#20
De-Min Ma, Dian-Xi Luo, Jie Zhang
BACKGROUND: More recent studies have revealed that chemokine receptor CXCR7 plays an important role in cancer development. However, little is known about the effect of CXCR7 on the process of gastric cancer cell invasion and angiogenesis. The aim of this study is to investigate the expression of CXCR7 in gastric cancer cell lines and to evaluate the role of CXCR7 in the proliferation, invasion, adhesion, and angiogenesis of gastric cancer cells. METHODS: Real-time PCR and Western blotting were used to examine the mRNA and protein levels of CXCR4 and CXCR7 in five gastric cancer cell lines (HGC-27, MGC-803, BGC-823, SGC-7901, and MKN-28)...
October 6, 2016: World Journal of Surgical Oncology
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