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Yun Liu, Chen Shao, Linqi Zhu, Sihong Jiang, Guanlin Li, Wei Zhang, Yajing Lin, Ying Ni, Hui Cao, Shihe Shao
BACKGROUND: Diseases associated with Abelson-related gene (also called ABL2) include leukemia; furthermore, previous researches have studied the expressions and functions of ABL2 in different types of malignancies and found that it plays an important role in almost all kinds of cancers. AIMS: Nevertheless, the mechanism of ABL2 in gastric cancer (GC) remains vague. METHODS: In the present study, the level of ABL2 in human GC tissues was detected by immunohistochemistry...
May 16, 2018: Digestive Diseases and Sciences
Lu Zhang, Fei Zheng, Zhigang Peng, Zijing Hu, Zhi Yang
Background: Cancer stem cell (CSC) promotes angiogenesis which plays an important role in tumor occurrence, growth, and metastasis. Accurately, quantifying the tumor vasculature can help understanding CSC characteristics and improve cancer diagnosis, therapy planning, and evaluation. The objective of this study is to present a method for improved angiogenesis assessment. Methods: We proposed a three-dimensional microvessel density (3D MVD) to evaluate tumor angiogenesis and tested it in animal models...
2018: Stem Cells International
Jingyi Yuan, Jiali Zeng, Chun Shuai, Yue Liu
Gastric cancer has a high incidence and mortality in the world, especially in China. The pathogenesis leading to the high heterogeneity of gastric cancer remains unclear. It is believed that TWSG1 is associated with a variety of tumors, but there are few studies related to gastric cancer. To investigate the biological significance of TWSG1, we evaluated the TWSG1 expression of the clinical samples and gastric cancer cell lines (BGC-823, MGC-803, and SGC-790) via stomach cancer tissue array, real-time PCR, and western blotting...
May 14, 2018: DNA and Cell Biology
Zhilin Qi, Tuo Tang, Lili Sheng, Yunfei Ma, Yinhua Liu, Liang Yan, Shimei Qi, Liefeng Ling, Yao Zhang
Salidroside, an active ingredient extracted from the Rhodiola rosea plant, has potential anti‑tumor effects. However, the effects of salidroside on gastric cancer cell proliferation and migration remain unclear. In the present study, the inhibitory effects of salidroside on gastric cancer cell proliferation, migration and invasion and the molecular mechanisms underlying these effects were investigated. The human gastric cancer cell line, BGC‑823, was treated with different concentrations of salidroside (200, 400 and 600 µg/ml)...
May 3, 2018: Molecular Medicine Reports
Fei Ye, Yi Gong, Xiangheng Chen, Meiying Yu, Zhongkun Zuo, Dongni Pei, Wei Liu, Qunwei Wang, Jun Zhou, Lunxi Duan, Leiyi Zhang, Xiaojing Li, Tenglong Tang, Jiangsheng Huang
Long noncoding RNA serves important roles in gastric cancer (GC). However, the prognostic significance and tumorigenesis effect of AFAP1-antisense RNA 1 (AS1) in GC remain to be clarified. The present study was conducted in order to determine the expression level of AFAP1-AS1 by reverse transcription-quantitative polymerase chain reaction. It was demonstrated that AFAP1-AS1 expression level was higher in GC tissues in comparison with adjacent tissues. By analyzing 66 GC tissue specimens, AFAP1-AS1 expression level was found to be markedly associated with tumor size, clinical stage and differentiation...
May 2018: Oncology Letters
Hai-Bo Yang, Hui-Ru Gao, Yuan-Jing Ren, Fei-Xiang Fang, Hong-Tao Tian, Zhen-Jiang Gao, Wei Song, Shao-Min Huang, An-Fang Zhao
Resistance to apoptosis is an characteristic of cancer cells that serves a critical function in tumor development and represents a target for antitumor therapy. Isoimperatorin (ISOIM), a coumarin compound, exhibits antitumor functions in multiple types of tumor cells. However, its antitumor effects and molecular mechanisms with respect to gastric cancer have not been elucidated. The present study assessed the anti-proliferative and apoptotic effects of ISOIM on human BGC-823 gastric cancer cells and elucidated its underlying molecular mechanisms...
May 2018: Oncology Letters
Yuqiong Zhang, Meiqin Xu, Xueyi Zhang, Fuying Chu, Tianji Zhou
BARF1 , encoded by Epstein-Barr virus (EBV), has been hypothesized to function as an oncogene, which was expressed in gastric carcinoma cells. Additionally, it has been reported that the anti-apoptotic function is closely associated with the expression of the B-cell lymphoma-2 (Bcl-2) protein. In addition, the signaling pathway has been reported to be involved in numerous diseases, including the mitogen-activated protein kinase (MAPK) cascade. In order to study the specific mechanism of anti-apoptotic function, BARF1 -stably-expressing immortalized normal human embryo gastric epithelial cell line GES1 (GES- BARF1 ), and well-, moderately- and poorly-differentiated gastric carcinoma cell lines, MKN28 (which has been reported to be contaminated with the moderately-differentiated MKN74 gastric carcinoma cell line), SGC7901 and BGC823 (MKN- BARF1 , SGC- BARF1 and BGC- BARF1 , respectively) (GCC- BARF1 ) were constructed, with transfection of cells with the empty vector pSG5 acting as controls...
May 2018: Oncology Letters
Junnan Feng, Chuanke Zhao, Lixin Wang, Like Qu, Hua Zhu, Zhi Yang, Guo An, Huifang Tian, Chengchao Shou
Angiogenesis plays a critical role in tumor aggressiveness, and a lot of anti-angiogenic agents have been used in clinical therapy. The therapeutic efficacy of peptides are generally restricted by the short in vivo life-time, thus, we were interested in developing a novel albumin-based and maleimidopropionic acid-conjugated peptide to prolong the half-life and improve the anti-tumor effect. Methods: We developed a peptide F56 with a maleimidopropionic acid (MPA) at the C-terminal (denoted as F56-CM), which allows immediate and irreversible conjugation with serum albumin...
2018: Theranostics
Yeqian Zhang, Site Yu, Zizhen Zhang, Gang Zhao, Jia Xu
Gastric cancer is one of the major causes of cancer death worldwide; however, the mechanism of carcinogenesis is complex and poorly understood. Long noncoding RNA (lncRNA) have been reported to be involved in the development of multiple cancers. Here we identified a novel lncRNA, AK096174, which was upregulated and associated with tumorigenesis, tumor size, metastasis, and poor prognosis in gastric cancer. Our data showed that AK096174 was highly expressed in the gastric cancer tissues and cell lines (SGC-7901, AGS, BGC-823, MGC-803), and patients with higher AK096174 expression had a poorer prognosis and shorter overall survival...
May 1, 2018: Bioscience Reports
Qingran Meng, Yaqi Wang, Feng Chen, Tiancun Xiao, Lianfu Zhang
This study discusses the optimization of the microwave-assisted extraction of polysaccharides from Diaphragma juglandis (DJPs). One main fraction (DJP-2) was successfully purified by ion-exchange chromatography and gel-permeation chromatography. The results showed that the optimal extraction conditions with DJP extraction yield of 4.7 ± 0.28% were water to raw material ratio of 20 mL/g, extraction time of 40 min, and microwave extraction power of 400 W. Bioassays indicated that DJP-2 could effectively suppress the proliferation of HepG2 and BGC-82 cell lines...
April 26, 2018: International Journal of Biological Macromolecules
Changcai Bai, Xiuping Zhou, Lu Han, Yongjie Yu, Nan Li, Ming Zhang, Zhuo Qu, Pengfei Tu
Two novel 18,19-seco-ursane triterpenoid saponins, ilexasprellanosides J-H (1-2, resp.), 3-O-α-l-Rhamnopyranosyl-(1 → 2)-β-d-xylopyrannosyl-19-O-β-d-glucopyranosyl-16-β-hydroxyl-18,19-seco-13(18)-urs-ene-21, 28-lactone (1), 3-O-β-d-Xylopyrannosyl-19-O-α-l-rhamnopyran osyl-(1 → 2)-α-l-arabinopyranoside-16, 21-epoxy-18, 19-seco-13(18)-urs-ene-28-oic acid (2), five known compounds (3-7) were isolated from the leaves of Ilex asprella (Hook. et Arn.) Champ. ex Benth. (Gangmeiye). The chemical structures of these compounds were elucidated through UV, IR, ESI-MS, 1 H NMR and 13 C NMR analyses...
April 23, 2018: Fitoterapia
Tom Hayon Eyles, Natalia Miguel Vior, Andrew W Truman
Heterologous expression of biosynthetic gene clusters (BGCs) represents an attractive route to the production of new natural products, but is often hampered by poor yields. It is therefore important to develop tools that enable rapid refactoring, gene insertion/deletion and targeted mutations in BGCs. Ideally, these tools should be highly efficient, affordable, accessible, marker free, and flexible for use with a wide range of BGCs. Here, we present a one-step yeast-based method that enables efficient, cheap and flexible modifications to BGCs...
April 25, 2018: ACS Synthetic Biology
Yi Zhe Li, Ting Long Zhang, Qiu Yu Liu, Ying Li
The ecological process models are powerful tools for studying terrestrial ecosystem water and carbon cycle at present. However, there are many parameters for these models, and weather the reasonable values of these parameters were taken, have important impact on the models simulation results. In the past, the sensitivity and the optimization of model parameters were analyzed and discussed in many researches. But the temporal and spatial heterogeneity of the optimal parameters is less concerned. In this paper, the BIOME-BGC model was used as an example...
January 2018: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
Joshua L Mieher, Matthew R Larson, Norbert Schormann, Sangeetha Purushotham, Ren Wu, Kanagalaghatta R Rajashankar, Hui Wu, Champion Deivanayagam
The high-resolution structure of Glucan binding protein C (GbpC) at 1.14 Å, a sucrose-dependent virulence factor of the dental caries pathogen Streptococcus mutans , has been determined. GbpC not only shares structural similarities with the V-regions of AgI/II and SspB, but also functional adherence to SAG and its scavenger receptor cysteine rich domains (SRCRs) which is not only a newly identified function for GbpC, but also an additional fail-safe binding mechanism for S. mutans Despite the structural similarities with S...
April 23, 2018: Infection and Immunity
Xuelian Cui, Liyan Chen, Jie Sun, Yixuan Wang, Zhenhua Lin
Objective To investigate the effect and molecular mechanism of β-lapachone combined with NVP-BEZ235 on the proliferation and migration of BGC-823 human gastric cancer cells. Methods BGC-823 cells were randomly divided into four groups: control group, 1 μmol/L β-lapachone group, 50 nmol/L NVP-BEZ235 group and 1 μmol/L β-lapachone combined with 50 nmol/L NVP-BEZ235 group. The proliferation of cells was determined using the MTT assay and colony formation assay. The expression levels of proliferation-related proteins phosphorylated AKT (p-AKT), phosphorylated NF-κB (p-NF-κB), phosphorylated extracellular signal-regulated kinase (p-ERK) and cyclin D1 were detected by Western blotting...
February 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
Xue Wang, Haibo Zhou, Hanna Chen, Xiaoshu Jing, Wentao Zheng, Ruijuan Li, Tao Sun, Jiaqi Liu, Jun Fu, Liujie Huo, Yue-Zhong Li, Yuemao Shen, Xiaoming Ding, Rolf Müller, Xiaoying Bian, Youming Zhang
Bacterial genomes encode numerous cryptic biosynthetic gene clusters (BGCs) that represent a largely untapped source of drugs or pesticides. Mining of the cryptic products is limited by the unavailability of streamlined genetic tools in native producers. Precise genome engineering using bacteriophage recombinases is particularly useful for genome mining. However, recombinases are usually host-specific. The genome-guided discovery of novel recombinases and their transient expression could boost cryptic BGC mining...
April 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
Zhen Liu, Jingyi Zhao, Xiao Liang, Xiaoxi Lv, Yong Li, Jing Qu, Yunbao Liu
Nine new compounds (1-6 and 16-18) and nine known compounds (7-15) were isolated from the Diaporthe pseudomangiferaea, an endophytic fungus obtained from the leaves of the toxic Chinese folk medicine Tylophora ouata. Their structures were elucidated by NMR spectroscopy and MS spectrometry analyses. The absolute configurations were established according to the specific rotation or electron circular dichroism method. Compounds 1, 4, 9, 11, 14 and 15 inhibited the TFG-β induced activation of human lung fibroblasts MRC-5 cells by 17...
April 13, 2018: Fitoterapia
Yaqin Liu, Tao Li, Hongshi Ma, Dong Zhai, Cuijun Deng, Jinwu Wang, Shangjun Zhuo, Jiang Chang, Chengtie Wu
For treatment of bone tumor and regeneration of bone defects, the biomaterials should possess the ability to kill tumor cells and regenerate bone defect simultaneously. To date, there are a few biomaterials possessing such dual functions, the disadvantages, however, such as long-term toxicity and degradation, restrict their application. Although bioactive elements have been incorporated into biomaterials to improve their osteogenic activity, there is no report about elements-induced functional scaffolds for photothermal tumor therapy...
April 12, 2018: Acta Biomaterialia
Tim W Malisch, Osama O Zaidat, Alicia C Castonguay, Franklin A Marden, Rishi Gupta, Chung-Huan J Sun, Coleman O Martin, William E Holloway, Nils Mueller-Kronast, Joey English, Italo Linfante, Guilherme Dabus, Hormozd Bozorgchami, Andrew Xavier, Ansaar T Rai, Michael Froehler, Aamir Badruddin, Thanh N Nguyen, M Asif Taqi, Michael G Abraham, Vallabh Janardhan, Hashem Shaltoni, Robin Novakovic, Albert J Yoo, Alex Abou-Chebl, Peng Roc Chen, Gavin W Britz, Ritesh Kaushal, Ashish Nanda, Raul G Nogueira
Background: Various techniques are used to enhance the results of mechanical thrombectomy with stent-retrievers, including proximal arrest with balloon guide catheter (BGC), conventional large bore proximal catheter (CGC), or in combination with local aspiration through a large-bore catheter positioned at the clot interface (Aspiration-Retriever Technique for Stroke [ARTS]). We evaluated the impact of ARTS in the North American Solitaire Acute Stroke (NASA) registry. Summary: Data on the use of the aspiration technique were available for 285 anterior circulation patients, of which 29 underwent ARTS technique, 131 CGC, and 125 BGC...
February 2018: Interventional Neurology
Chao Liu, Min Jian, Hong Qi, Wei-Zheng Mao
Recently, microRNAs (miRNAs) have reported to participate in multiple biological processes. However, the effects of miR-495 on gastric cancer (GC) remain unclear. The purpose of this study is to explore the functions of miR-495 in GC cells proliferation, metastasis and apoptosis. SGC-7901 and BGC-823 were transfected with miR-495 mimic, miR-495 inhibitor and negative controls (mimic control and inhibitor control). The expressions of miR-495, cell viability, migration, apoptosis and apoptosis-related factors were examined by qRT-PCR, trypan blue staining, Transwell, flow cytometry and western blot, respectively...
April 3, 2018: Oncology Research
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