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https://www.readbyqxmd.com/read/28649863/cytotoxic-triterpenoid-saponins-from-the-defatted-seeds-of-camellia-oleifera-abel
#1
Hui-Zheng Fu, Kai-Hua Wan, Qing-Wei Yan, Guo-Ping Zhou, Tong-Tong Feng, Mian Dai, Rui-Jian Zhong
Five new oleanane-type triterpenoid saponins, oleiferasaponins D1-D5 (1-5), were isolated from the defatted seeds of Camellia oleifera Abel. Their structures were elucidated by spectroscopic and chemical methods. The cytotoxic activities of compounds 1-5 were evaluated against five human tumor cell lines (HCT-116, HepG2, BGC-823, NCI-H1650, and A2780). Compounds 1-2 exhibited cytotoxic activity against five human cancer cell lines, with IC50 values ranging from 3.31 to 10.23 μM. Compounds 3-5 showed moderate cytotoxic activities toward the tested cell lines...
June 25, 2017: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/28639665/isolation-and-identification-of-l-d-lactate-conjugated-bufadienolides-from-toad-eggs-revealing-lactate-racemization-in-amphibians
#2
Shiwen Zhou, Qingfei Zheng, Xiuyong Huang, Yong Wang, Sifan Luo, Renwang Jiang, Lei Wang, Wencai Ye, Haiyan Tian
Three pairs of bufadienolide l/d-lactate epimers (1-6) were isolated from the eggs of the toad Bufo bufo gargarizans. The structures were elucidated by using spectroscopic methods, X-ray diffraction analysis and a modified Mosher's method. Compounds 1-6 represent the first occurrence of lactate-conjugated bufadienolides in nature, and illustrate the existence of an enzyme-controlled epimerization from l- to d-lactate in amphibians. The biosynthetic pathways, in which two key enzymes might be involved (i.e., lactate racemase and acyltransferase), were proposed...
June 22, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28618940/association-of-wnt1-inducible-signaling-pathway-protein-1-with-the-proliferation-migration-and-invasion-in-gastric-cancer-cells
#3
Shuqin Jia, Tingting Qu, Mengmeng Feng, Ke Ji, Ziyu Li, Wenguo Jiang, Jiafu Ji
Wnt1-inducible signaling pathway protein-1 is a cysteine-rich protein that belongs to the CCN family, which has been implicated in mediating the occurrence and progression through distinct molecular mechanisms in several tumor types. However, the association of Wnt1-inducible signaling pathway protein-1 with gastric cancer and the related molecular mechanisms remain to be elucidated. Therefore, this study aimed to clarify the biological role of Wnt1-inducible signaling pathway protein-1 in the proliferation, migration, and invasion in gastric cancer cells and further investigated the associated molecular mechanism on these biological functions...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28618401/64cu-psma-617-a-novel-psma-targeted-radio-tracer-for-pet-imaging-in-gastric-adenocarcinoma-xenografted-mice-model
#4
Xue-di Han, Chen Liu, Fei Liu, Qing-Hua Xie, Te-Li Liu, Xiao-Yi Guo, Xiao-Xia Xu, Xing Yang, Hua Zhu, Zhi Yang
Here, we report that it's feasible for imaging gastric adenocarcinoma mice model with prostate-specific membrane antigen (PSMA) targeting imaging agents, which could potentially provide an alternate and readily translational tool for managing gastric adenocarcinoma. DKFZ-PSMA-617, a PSMA targeting ligand reported recently, was chosen to be radio-labeled with nuclide 64Cu. 64Cu-PSMA-617 was radio-synthesized in high radio-chemical yield and specific activity up to 19.3 GBq/μmol. It showed good stability in vitro...
May 26, 2017: Oncotarget
https://www.readbyqxmd.com/read/28615102/-effect-of-golgi-%C3%AE-mannosidase-2-gm2-gene-knockdown-on-adhesion-abilities-of-human-gastric-carcinoma-cell-line-bgc-823-and-its-mechanism
#5
Bo Zeng, Zhen Zeng, Chang Liu, Yaying Yang
Objective To investigate the effect of Golgi α-mannosidase II (GM2) gene knockdown on adhesion abilities of BGC-823 human gastric carcinoma cells. Methods Three plasmid vectors expressing GM2 shRNAs and a negative control plasmid vector were designed, constructed and then transfected into BGC-823 cells by Lipofectamine(TM) 2000. After transfection, the mRNA and protein levels of GM2 in BGC-823 cells were detected by real-time quantitative PCR (qRT-PCR) and Western blotting to evaluate the transfection efficacy...
June 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28606333/sex-estimation-from-the-scapula-in-a-contemporary-thai-population-applications-for-forensic-anthropology
#6
Tanya R Peckmann, Shelby Scott, Susan Meek, Pasuk Mahakkanukrauh
The impact of climate change is estimated to be particularly severe in Thailand. Overall, the country faces an increase in surface temperatures, severe storms and floods, and a possible increase in the number of mass disasters in the region. It is extremely important that forensic scientists have access to sex estimation methods developed for use on a Thai population. The goal of this project is to evaluate the accuracy of sex estimation discriminant functions, created using contemporary Mexican and Greek populations, when applied to a contemporary Thai sample...
July 2017: Science & Justice: Journal of the Forensic Science Society
https://www.readbyqxmd.com/read/28598080/-expression-of-cxcr7-in-gastric-cancer-cells-and-its-effect-on-the-migration-and-invasion-of-sgc-7901-cells
#7
Hai-Tao Shi, A-Meng Shi, Yan Wang, Lei Dong, Gang Zhao, Yong Li, Xiao-Lan Lu
OBJECTIVES: To determine the expression of chemokine (C-X-C motif) receptor 7 (CXCR7) in five gastric cancer cell lines with various degrees of differentiation, and the effect of silencing CXCR7 on the migration and invasion of SGC-7901 cells. METHODS: The expression of CXCR7 in gastric cell lines (HGC-27, MGC-803, SGC-7901, BGC-823 and MKN-28) was detected by Western bolt and RT-PCR. The SGC-7901 cells were transfected with liposome of CXCR7 siRNA to silence CXCR7 gene, and then treated with stromal-derived factor-1 (SDF-1)-the ligand of CXCR7...
September 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28589920/systematic-evaluation-of-the-osteogenic-capacity-of-low-melting-bioactive-glass-reinforced-45s5-bioglass-porous-scaffolds-in-rabbit-femoral-defects
#8
Lei Zhang, Xiurong Ke, Lingzhi Lin, Jun Xiao, Xianyan Yang, Juncheng Wang, Guojing Yang, Sanzhong Xu, Zhongru Gou, Zhanjun Shi
Due to the low strength and high brittleness of 45S5 Bioglass(®), it is still a great challenge for the three-dimensional porous 45S5 Bioglass(®) to treat mechanically required loaded bone defects. Therefore, 45S5 Bioglass(®)-derived bioactive glass-ceramic (BGC) porous scaffolds were fabricated at a low temperature sintering condition with and without the addition of 4% low-melting ZnO/B2O3 (ZB) bioactive glass as a reinforcing agent and using 350- or 500 μm paraffin microspheres as a porogen. The pore structure characterization for the scaffolds indicated that the scaffolds containing 4% ZB had very good macroporous structures of ∼313 and ∼448 μm in pore size and over 70% porosity with appreciable strength (>15 MPa), which was about four times higher than that those manufactured without ZB and with 350 μm porogen scaffolds...
June 7, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28588255/pkm2-promotes-cell-migration-and-inhibits-autophagy-by-mediating-pi3k-akt-activation-and-contributes-to-the-malignant-development-of-gastric-cancer
#9
Chao Wang, Jinling Jiang, Jun Ji, Qu Cai, Xuehua Chen, Yingyan Yu, Zhenggang Zhu, Jun Zhang
Pyruvate kinase M2 (PKM2) is a key kinase of glycolysis and is characteristic of all proliferating cells. The role of PKM2 in gastric cancer (GC) is still ambiguous and yet to be determined. To better understand the role of PKM2 in both the migration and invasion of GC, we measured the expression of PKM2 in GC cell lines using qRT-PCR and western blot. The prognostic value of PKM2 was analyzed by Immunohistochemistry in a cohort containing 88 GC patients. PKM2 was knocked down by the short hairpin RNA plasmid vector in NCI-N87 and BGC-823 cells, and the biological behavior and downstream signaling pathways were also investigated in vitro...
June 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28587290/interpreting-microbial-biosynthesis-in-the-genomic-age-biological-and-practical-considerations
#10
REVIEW
Ian J Miller, Marc G Chevrette, Jason C Kwan
Genome mining has become an increasingly powerful, scalable, and economically accessible tool for the study of natural product biosynthesis and drug discovery. However, there remain important biological and practical problems that can complicate or obscure biosynthetic analysis in genomic and metagenomic sequencing projects. Here, we focus on limitations of available technology as well as computational and experimental strategies to overcome them. We review the unique challenges and approaches in the study of symbiotic and uncultured systems, as well as those associated with biosynthetic gene cluster (BGC) assembly and product prediction...
June 6, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28586004/cox-2-regulates-snail-expression-in-gastric-cancer-via-the-notch1-signaling-pathway
#11
Yuwei Ye, Min Liu, Hao Yuan, Shupeng Ning, Yuping Wang, Zhaofeng Chen, Rui Ji, Qinghong Guo, Qiang Li, Yongning Zhou
The conversion of arachidonic acid into prostaglandins by cyclooxygenase (COX)-2 contributes to the biological properties of malignant tumours. During the initiation and development of various tumours, the Notch family plays a key role. However, the association between COX‑2 and the Notch family in gastric cancer (GC) remains unclear. The present study aimed to clarify the mechanisms through which COX‑2 participates in the pathogenesis of GC. Quantitative PCR and western blot analysis were used to detect the expression of Notch family members and COX‑2 in human GC and paracancerous tissues, GES‑1 cells and GC cell lines (AGS, SGC‑7901, BGC‑823, and MGC‑803) treated with or without celecoxib, prostaglandin E2 and small interfering RNA (siRNA)...
June 2, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28546810/a-downmodulated-microrna-profiling-in-patients-with-gastric-cancer
#12
Tao Zhang, Chang Liu, Shi Huang, Yuanping Ma, Jiansong Fang, Yuanneng Chen
Objective. Here, we aim to investigate the microRNA (miR) profiling in human gastric cancer (GC). Methods. Tumoral and matched peritumoral gastric specimens were collected from 12 GC patients who underwent routine surgery. A high-throughput miR sequencing method was applied to detect the aberrantly expressed miRs in a subset of 6 paired samples. The stem-loop quantitative real-time polymerase chain reaction (qRT-PCR) assay was subsequently performed to confirm the sequencing results in the remaining 6 paired samples...
2017: Gastroenterology Research and Practice
https://www.readbyqxmd.com/read/28506699/cra-crosolic-acid-isolated-from-actinidia-valvata-dunn-radix-induces-apoptosis-of-human-gastric-cancer-cell-line-bgc823-in%C3%A2-vitro-via-down-regulation-of-the-nf-%C3%AE%C2%BAb-pathway
#13
Qi-Lai Cheng, Hong-Liang Li, Ying-Chen Li, Zhao-Wen Liu, Xiao-Hua Guo, Yi-Jian Cheng
A natural ursolic compound, 2α,3β-dihydroxy-urs-12-en-28-oic acid (corosolic acid, CRA) was isolated from the root of Actinidia valvata Dunn. (A. valvata Radix). Since a large number of triterpenoid compound has marked anticancer effects toward various types of cancer cell lines in vitro, this study was carried out to investigate the anticancer effect of CRA in human gastric cancer cell line BGC823 cells and the underlying apoptotic mechanism of CRA was examined in BGC823 cell lines. The results showed that CRA significantly suppressed the viability of BGC823 cells in a concentration- and time-dependent manner...
May 12, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28506467/apoptosis-of-bovine-granulosa-cells-intracellular-pathways-and-differentiation
#14
M C Carou, P R Cruzans, A Maruri, M G Farina, C D Fiorito, G Olea, D M Lombardo
Follicular atresia in granulosa and theca cells occurs by apoptosis through weak hormonal stimulation. We have previously proposed an in vitro model to study this process by inducing apoptosis in BGC-1, a bovine granulosa cell line, and in primary cultures from ovaries with or without corpus luteum (CPGB+ and CPGB-, respectively), with different doses of gonadotropin releasing hormone (GnRH) analogs (leuprolide acetate (LA) as agonist and antide as antagonist). BGC-1 represent immature granulosa cells, whereas CPGB represent different degrees of luteinization...
May 12, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28498433/curcumin-induces-apoptotic-cell-death-and-protective-autophagy-in-human-gastric-cancer-cells
#15
Wei Li, Ying Zhou, Jin Yang, Haining Li, Huanhuan Zhang, Ping Zheng
Curcumin possesses an anticancer effect against a wide assortment of tumors with selective cytotoxicity for tumor cells. However, the mechanism involved in the curcumin‑induced anticancer effect remain unclear. In the present study, we investigated the efficacy of curcumin against human gastric cancer cell growth and the molecular mechanism involved. Our results demonstrated that curcumin inhibited the viabilities of gastric cancer cell lines BGC-823, SGC-7901 and MKN-28 in both a time- and dose-dependent manner...
May 11, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28485805/microrna-217-suppressed-epithelial-to-mesenchymal-transition-in-gastric-cancer-metastasis-through-targeting-ptpn14
#16
Y-P Liu, X-H Sun, X-L Cao, W-W Jiang, X-X Wang, Y-F Zhang, J-L Wang
OBJECTIVE: Gastric cancer (GC) is the third leading cause of cancer-related deaths while the mechanisms underlying its metastasis are not fully understood. In this study, we aimed to explore the relationship between miR-217 and GC metastasis. PATIENTS AND METHODS: We examined miR-217 level in gastric tumor tissues of 48 patients with GC and in cell lines including gastric mucosa epithelial cell line (GES-1), gastric cancer cell line (BGC-823), and gastric cancer cell line (SGC-7901)...
April 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28476678/anticancer-efficacy-of-the-ethyl-acetate-extract-from-the-traditional-chinese-medicine-herb-celastrus-orbiculatus-against-human-gastric-cancer
#17
Haibo Wang, Lide Tao, Tengyang Ni, Hao Gu, Feng Jin, Xiaojun Dai, Jun Feng, Yanbing Ding, Weiming Xiao, Shiyu Guo, Tadashi Hisamitsu, Yayun Qian, Yanqing Liu
ETHNOPHARMACOLOGICAL RELEVANCE: The traditional Chinese medicine (TCM) herb Celastrus orbiculatus is an important folk medicinal plant in China that has been used as an anti-inflammatory, antitumor, and analgesic in various diseases. The ethyl acetate extract of C. orbiculatus (C. orbiculatus extract, COE) was reported to show significant antitumor effects. However, no study in China or abroad has reported the effect and mechanism of COE in triggering apoptosis of gastric cancer (GC) cells...
May 2, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28475004/knockdown-of-long-non-coding-rna-hoxd-as1-inhibits-gastric-cancer-cell-growth-via-inactivating-the-jak2-stat3-pathway
#18
Li Zheng, Jiangtao Chen, Zhongyong Zhou, Zhikuan He
Long non-coding RNA HOXD-AS1 (HOXD cluster antisense RNA 1) has been demonstrated to be closely associated with the progression of several tumors. However, the biological function of HOXD-AS1 and the underlying molecular mechanism in gastric cancer are still unclear. The expression of HOXD-AS1 in gastric cancer cell lines was evaluated by quantitative real-time polymerase chain reaction. The association of HOXD-AS1 expression and clinical parameters was statistically analyzed by chi-square test. Cell viability, colony formation capacity, and phosphorylation of Janus kinase 2 and signal transducer and activator of transcription 3 in treated SGC-7901 and BGC-823 cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, and western blot analysis, respectively...
May 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28472505/the-antibiotic-resistant-target-seeker-arts-an-exploration-engine-for-antibiotic-cluster-prioritization-and-novel-drug-target-discovery
#19
Mohammad Alanjary, Brent Kronmiller, Martina Adamek, Kai Blin, Tilmann Weber, Daniel Huson, Benjamin Philmus, Nadine Ziemert
With the rise of multi-drug resistant pathogens and the decline in number of potential new antibiotics in development there is a fervent need to reinvigorate the natural products discovery pipeline. Most antibiotics are derived from secondary metabolites produced by microorganisms and plants. To avoid suicide, an antibiotic producer harbors resistance genes often found within the same biosynthetic gene cluster (BGC) responsible for manufacturing the antibiotic. Existing mining tools are excellent at detecting BGCs or resistant genes in general, but provide little help in prioritizing and identifying gene clusters for compounds active against specific and novel targets...
May 2, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28463421/aromatic-polyketide-gtri-02-is-a-previously-unidentified-product-of-the-act-gene-cluster-in-streptomyces-coelicolor%C3%A2-a3-2
#20
Changsheng Wu, Koji Ichinose, Young Hae Choi, Gilles P van Wezel
The biosynthesis of aromatic polyketides derived from type II polyketide synthases (PKSs) is complex, and it is not uncommon that highly similar gene clusters give rise to diverse structural architectures. The act biosynthetic gene cluster (BGC) of the model actinomycete Streptomyces coelicolor A3(2) is an archetypal type II PKS. Here we show that the act BGC also specifies the aromatic polyketide GTRI-02 (1) and propose a mechanism for the biogenesis of its 3,4-dihydronaphthalen-1(2H)-one backbone. Polyketide 1 was also produced by Streptomyces sp...
April 30, 2017: Chembiochem: a European Journal of Chemical Biology
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