Read by QxMD icon Read


Sean Doyle, Gary W Jones, Stephen K Dolan
Gliotoxin is an epipolythiodioxopiperazine (ETP) class toxin, contains a disulfide bridge that mediates its toxic effects via redox cycling and is produced by the opportunistic fungal pathogen Aspergillus fumigatus. The gliotoxin bis-thiomethyltransferase, GtmA, attenuates gliotoxin biosynthesis in A. fumigatus by conversion of dithiol gliotoxin to bis-thiomethylgliotoxin (BmGT). Here we show that disruption of dithiol gliotoxin bis-thiomethylation functionality in A. fumigatus results in significant remodelling of the A...
April 2018: Fungal Biology
Furong Wang, Xiaoying Guan, Jinwei Yang, Wenting He, Yucai Wei, Hao Chen, Yumin Li
BACKGROUND: We investigated the expression of endoplasmic reticulum Golgi intermediate compartment 1 (ERGIC1) in precancerous gastric lesions and gastric cancer and the function of ERGIC in human gastric cancer cell lines. MATERIALS AND METHODS: A total of 160 subjects were enrolled. The expression of ERGIC1 was assayed using immunohistochemistry. Overexpression of ERGIC1 in SGC-7901 and BGC-823 cells was used to evaluate the function of ERGIC1. RESULTS: Most normal gastric mucosal tissues and the tissues with mild dysplasia showed strong expression of ERGIC1 (80% and 73...
March 2018: American Journal of the Medical Sciences
Kehilwe C Nakedi, Bridget Calder, Mousumi Barnejee, Alexander Giddey, Andrew Jm Nel, Shaun Garnett, Jonathan M Blackburn, Nelson A Da Cruz Soares
Mycobacterial Ser/Thr kinases play a critical role in bacterial physiology and pathogenesis. Linking kinases to the substrates they phosphorylate in vivo, thereby elucidating their exact functions, is still a challenge. The aim of this work was to associate protein phosphorylation in mycobacteria with important subsequent macro cellular events by identifying the physiological substrates of PknG in Mycobacterium bovis BCG.  The study compared the phosphoproteome dynamics during the batch growth of M. bovis BGC versus the respective PknG knock-out mutant (ΔPknG-BCG) strains...
March 16, 2018: Molecular & Cellular Proteomics: MCP
Christian Greunke, Elke Regina Duell, Paul Michael D'Agostino, Anna Glöckle, Katharina Lamm, Tobias Alexander Marius Gulder
Specialized metabolites from bacteria are an important source of inspiration for drug development. The genes required for the biosynthesis of such metabolites in bacteria are usually organized in so-called biosynthetic gene clusters (BGCs). Using modern bioinformatic tools, the wealth of genomic data can be scanned for such BGCs and the expected products can often structurally be predicted in silico. This facilitates the directed discovery of putatively novel bacterial metabolites. However, the production of these molecules often requires genetic manipulation of the BGC for activation or the expression of the pathway in a heterologous host...
March 13, 2018: Metabolic Engineering
R Cameron Coates, Benjamin P Bowen, Ernst Oberortner, Linda Thomashow, Michalis Hadjithomas, Zhiying Zhao, Jing Ke, Leslie Silva, Katherine Louie, Gaoyan Wang, David Robinson, Angela Tarver, Matthew Hamilton, Andrea Lubbe, Meghan Feltcher, Jeffery L Dangl, Amrita Pati, David Weller, Trent R Northen, Jan-Fang Cheng, Nigel J Mouncey, Samuel Deutsch, Yasuo Yoshikuni
Increasing availability of new genomes and putative biosynthetic gene clusters (BGCs) has extended the opportunity to access novel chemical diversity for agriculture, medicine, environmental and industrial purposes. However, functional characterization of BGCs through heterologous expression is limited because expression may require complex regulatory mechanisms, specific folding or activation. We developed an integrated workflow for BGC characterization that integrates pathway identification, modular design, DNA synthesis, assembly and characterization...
March 15, 2018: Journal of Industrial Microbiology & Biotechnology
Hao Wang, Mingxia Zhang, Guoping Sun
This study aimed to elucidate the roles of long non-coding RNA Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) in gastric cancer. The expression of NEAT1 in gastric cancer tissues and cells was determined. NEAT1 was then overexpressed and suppressed in BGC-823 cells in vitro to further explore the effects of NEAT1 on cell proliferation, migration, and invasion. In addition, the regulatory relationship among NEAT1, miR-335-5p and Rho Associated Coiled-Coil Containing Protein Kinase 1 (ROCK1) was investigated...
March 5, 2018: Die Pharmazie
Liang Liang, Ming Zeng, Haixia Pan, Hao Liu, Yangke He
Previous studies have demonstrated that nicotinamide N-methyltransferase (NNMT) is aberrantly expressed in a number of tumors. In the present study, it was demonstrated that the gene and protein levels of NNMT were significantly increased in gastric cancer cells. Furthermore, upregulation of NNMT significantly increased the expression of mesenchymal markers, including α-smooth muscle actin (SMA), vimentin and fibronectin, but decreased the levels of epithelial cadherin. Since transforming growth factor (TGF)-β1 may serve a key function in epithelial-mesenchymal transition (EMT), the effects of NNMT on the expression of TGF-β1 were investigated in BGC-823 cells...
April 2018: Oncology Letters
Min Sha, Mei Lin, Jia Wang, Jun Ye, Jie Xu, Ning Xu, Junxing Huang
BACKGROUND: The objective of this study was to investigate the role and mechanism of long non-coding RNA MIAT in gastric cancer (GC). METHODS: Real-time PCR was used to determine MIAT level in 120 GC tissues, and in two gastric cancer cell lines. The clinicopathological characteristics of MIAT in GC patients were analyzed. Small interfering RNA specific for MIAT (si-MIAT) and lentivector for si-MIAT was performed to down-regulate MIAT expression in GC cells and in animal tumor model, respectively...
March 14, 2018: Journal of Experimental & Clinical Cancer Research: CR
Xing-Xing He, Chen-Kai Huang, Bu-Shan Xie
The exact molecular mechanism of 5-fluorouracil (5-FU) in human gastric cancer cells remains to be elucidated. Cultured BGC‑823 human gastric carcinoma and AGS cell lines were treated with 5‑FU. Autophagosome formation was investigated through multiple approaches, including the quantification of green fluorescent protein‑microtubule‑associated protein 1A/1B‑light chain 3 (LC3) puncta, LC3 conversion and electron microscopy observations. Additionally, autophagy was inhibited using 3‑methyladenine (3‑MA) and beclin‑1 ablation, to determine its role in 5‑FU‑mediated cell death...
March 1, 2018: Molecular Medicine Reports
Douglas R Cohen, Craig A Townsend
Despite the identification of a β-hydroxyhexaene produced by the enediyne polyketide synthases (PKSs), the post-PKS biosynthetic steps to the individual members of this antitumor antibiotic family remain largely unknown. The massive biosynthetic gene clusters (BGCs) that direct the formation of each product caution that many steps could be required. It was recently demonstrated that the enediyne PKS in the dynemicin A BGC from Micromonospora chersina gives rise to both the anthraquinone and enediyne "halves" of the molecule...
March 7, 2018: Angewandte Chemie
Si-Sun Choi, Yohei Katsuyama, Linquan Bai, Zixin Deng, Yasuo Ohnishi, Eung-Soo Kim
The discovery and development of microbial natural products (MNPs) have played pivotal roles in the fields of human medicine and its related biotechnology sectors over the past several decades. The post-genomic era has witnessed the development of microbial genome mining approaches to isolate previously unsuspected MNP biosynthetic gene clusters (BGCs) hidden in the genome, followed by various BGC awakening techniques to visualize compound production. Additional microbial genome engineering techniques have allowed higher MNP production titers, which could complement a traditional culture-based MNP chasing approach...
March 3, 2018: Current Opinion in Microbiology
Elizabeth I Parkinson, James H Tryon, Anthony W Goering, Kou-San Ju, Ryan A McClure, Jeremy D Kemball, Sara Zhukovsky, David P Labeda, Regan J Thomson, Neil L Kelleher, William W Metcalf
Natural products (NPs) are a rich source of medicines, but traditional discovery methods are often unsuccessful due to high rates of rediscovery. Genetic approaches for NP discovery are promising, but progress has been slow due to the difficulty of identifying unique biosynthetic gene clusters (BGCs) and poor gene expression. We previously developed the metabologenomics method, which combines genomic and metabolomic data to discover new NPs and their BGCs. Here, we utilize metabologenomics in combination with molecular networking to discover a novel class of NPs, the tyrobetaines: nonribosomal peptides with an unusual trimethylammonium tyrosine residue...
March 13, 2018: ACS Chemical Biology
Xiaofeng Xu, Feng Gao, Jianjiang Wang, Lan Tao, Jinsong Ye, Li Ding, Wei Ji, Xing Chen
OBJECTIVE: To explore the relationship between miR-122-5p and DUSP4 and their effects on gastric cancer (GC) cell mobility and invasiveness. METHODS: Abnormally expressed miRNAs and mRNAs were analyzed using microarrays. The miR-122-5p and DUSP4 mRNA expression levels in GC tissues and cells were determined by RT-qPCR. The target relationship between miR-122-5p and DUSP4 was validated by dual luciferase reporter assay. GC cell mobility and invasiveness were respectively observed by wound healing assay and transwell invasion assay...
March 6, 2018: Cancer Biology & Therapy
Xi Liu, Ping Du, Lei Han, Anling Zhang, Kui Jiang, Qingyu Zhang
Gastric cancer is one of the most common cancers in the world; taxol displayed modest efficacy as first-line chemotherapy for gastric cancer, conversely, it has limitations used alone. β-catenin is a multifunctional oncogenic protein and the elevation in expression and activity of β-catenin has been implicated in many cancers. Therefore, we assume that the inhibition of β-catenin can enhanced the efficacy of taxol. The purpose of this study was to investigate the inhibitory effect of miR-200a mimics, FH535 combined with taxol on proliferation and invasion of human gastric cancer cell lines SGC-7901 and BGC-823...
December 6, 2017: Pathology, Research and Practice
Anna C Tottman, Frank H Bloomfield, Barbara E Cormack, Jane E Harding, M Atif Mohd Slim, Abigail F Weston, Jane M Alsweiler
OBJECTIVES: To determine whether changes to early nutrition are associated with levels of glycaemia in very preterm infants. METHODS: A retrospective, observational study of infants <1,500 grams or <30 weeks' gestation admitted to Neonatal Intensive Care, National Women's Hospital, NZ, before (Old Protocol) and after (New Protocol) a change in nutritional protocol. Nutritional intakes were calculated and averaged by day for postnatal days 1-7 (Week 1) and 1-28 (Month 1)...
February 23, 2018: Journal of Pediatric Gastroenterology and Nutrition
Jiajia Tu, Siting Li, Jiang Chen, Yongxiang Song, Shaobin Fu, Jianhua Ju, Qinglian Li
BACKGROUND: The deep-sea-derived microbe Streptomyces koyangensis SCSIO 5802 produces neoabyssomicins A-B (1-2) and abyssomicins 2 (3) and 4 (4). Neoabyssomicin A (1) augments human immunodeficiency virus-1 (HIV-1) replication whereas abyssomicin 2 (3) selectively reactivates latent HIV and is also active against Gram-positive pathogens including methicillin-resistant Staphylococcus aureus (MRSA). Structurally, neoabyssomicins A-B constitute a new subtype within the abyssomicin family and feature unique structural traits characteristic of extremely interesting biosynthetic transformations...
February 20, 2018: Microbial Cell Factories
Xiaobo Bo, Yusheng Chen, Weizhong Sheng, Yuda Gong, Haiyu Wang, Weidong Gao, Bo Zhang
Gastric cancer is a major cause of cancer-associated mortality worldwide. The aberrant expression of microRNA (miRNA) is involved in tumorigenesis. Ras proteins transfer information from the extracellular environment to internal cellular compartments and are essential in numerous signal transduction pathways. To investigate the regulation, function and clinical significance of the miRNA377/Ras association domain family (RASSF) 8 signaling axis in gastric cancer, reverse transcription-quantitative polymerase chain reaction, immunohistochemistry, cell counting kit-8, western blotting, and Transwell assays were used...
March 2018: Oncology Letters
Ling Du, Nathaniel Mikle, Zhenhua Zou, Yuanyuan Huang, Zheng Shi, Lifen Jiang, Heather R McCarthy, Junyi Liang, Yiqi Luo
Quantifying the ecological patterns of loss of ecosystem function in extreme drought is important to understand the carbon exchange between the land and atmosphere. Rain-use efficiency [RUE; gross primary production (GPP)/precipitation] acts as a typical indicator of ecosystem function. In this study, a novel method based on maximum rain-use efficiency (RUE max ) was developed to detect losses of ecosystem function globally. Three global GPP datasets from the MODIS remote sensing data (MOD17), ground upscaling FLUXNET observations (MPI-BGC), and process-based model simulations (BESS), and a global gridded precipitation product (CRU) were used to develop annual global RUE datasets for 2001-2011...
February 14, 2018: Science of the Total Environment
Xiaoting Jiang, Zhengchuang Liu, Yingjie Xia, Jungang Luo, Ji Xu, Xujun He, Houquan Tao
In this study, we investigated the role of Fat atypical cadherin 4 (FAT4) in gastric cancer (GC) progression. Immunohistochemical analysis showed lower FAT4 expression in tumor tissues from GC patients than in normal gastric epithelium. Lower FAT4 expression was associated with poor prognosis, tumor size and invasion, and lymph node and distant metastases. Multivariate analysis showed that TNM stage, lymph node and distant metastases, Lauren classification, and FAT4 expression were independent prognostic factors in GC...
January 12, 2018: Oncotarget
Hao Ren, Xiaomin Yang, Yongmei Yang, Xiaoyu Zhang, Rui Zhao, Ran Wei, Xin Zhang, Yi Zhang
Brain cytoplasmic RNA 1 (BCYRN1), along non-coding RNA, plays a critical role in various diseases, including some cancers. However, the expression of BCYRN1 and its roles in gastric carcinoma (GC) still remain unidentified. Thus, this study employed RT-qPCR to detect expression of BCYRN1 in 85 paired GC samples and adjacent normal tissues, and performed in vitro studies to explore effects of BCYRN1 in GC cells on cell proliferation, apoptosis and migration. We found BCYRN1 was significantly upregulated in GC samples, and its expression was positively correlated with advanced TNM stage (p = 0...
January 12, 2018: Oncotarget
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"