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Tristan T Watson, Tom A Forge, Louise M Nelson
Inoculation with antagonistic soil microorganisms has shown potential to suppress replant disease of apple in orchard soils. <i>Pseudomonas</i> spp. may have potential to reduce <i>Pratylenchus penetrans</i> populations on apple. <i>Pseudomonas</i> spp. were isolated from the rhizosphere of sweet cherry, screened for antagonistic characteristics, and two highly antagonistic <i>Pseudomonas</i> isolates, P10-32 and P10-42, were evaluated for growth promotion of apple seedlings, suppression of <i>P...
May 23, 2018: Canadian Journal of Microbiology
X Girerd, O Hanon, B Vaïsse
OBJECTIVE: A Visual Analog Scale (VAS) is useful for diagnosing medication nonadherence and its validity has been evaluated using electronic pillbox as the gold standard. We have developed the EvaLobs® scale for use on paper or on smartphone and the aim of the study was to administrate the scale among FLAHS 2017 participants treated for an hypertension, a dyslipidemia or diabetes. In subjects treated with antihypertensive medications, participants completed the 6-item Girerd Scale and EvaLobs® ...
May 18, 2018: Annales de Cardiologie et D'angéiologie
Sanaz Jarolmasjed, Lav R Khot, Sindhuja Sankaran
Bitter pit is one of the most important disorders in apples. Some of the fresh market apple varieties are susceptible to bitter pit disorder. In this study, visible⁻near-infrared spectrometry-based reflectance spectral data (350⁻2500 nm) were acquired from 2014, 2015 and 2016 harvest produce after 63 days of storage at 5 °C. Selected spectral features from 2014 season were used to classify the healthy and bitter pit samples from three years. In addition, these spectral features were also validated using hyperspectral imagery data collected on 2016 harvest produce after storage in a commercial storage facility for 5 months...
May 15, 2018: Sensors
Zhanxin Yao, Yeqing Gu, Qing Zhang, Li Liu, Ge Meng, Hongmei Wu, Yang Xia, Xue Bao, Hongbin Shi, Shaomei Sun, Xing Wang, Ming Zhou, Qiyu Jia, Yuntang Wu, Kun Song, Weina Gao, Changjiang Guo, Kaijun Niu
PURPOSE: Quercetin is one of potential antidiabetic substances because of its powerful antioxidant and anti-inflammatory actions. The purpose of this study is to estimate daily quercetin intake and assess the relationship between dietary quercetin intake and the prevalence of type 2 diabetes mellitus (T2DM) in a Chinese population. METHODS: Dietary intake was investigated by a validated 100-item food frequency questionnaire. Daily intakes of quercetin and nutrients were calculated accordingly...
May 12, 2018: European Journal of Nutrition
Patrycja Mojsak, Bożena Łozowicka, Piotr Kaczyński
This paper presents, for the first time, results for chlorpyrifos (CHLP) in Polish fruits and vegetables over the course of a long period of research, 2007-2016, with toxicological aspects. The challenge of this study was to re-evaluate the impact of chlorpyrifos residues in fruit and vegetables on health risk assessed via acute and chronic exposure based on old and new, lower, established values of: Average Daily Intakes (ADIs)/Acute Reference Doses (ARfDs) and Maximum Residue Levels (MRLs). A total of 3 530 samples were collected, and CHLP in the range of 0...
May 9, 2018: Ecotoxicology and Environmental Safety
M I Serqushkina, A N Khudyakov, T V Polezhaeva, O M Bezmeltseva
BACKGROUND: Pectins have a large number of active -OH and -COOH groups (3), suggesting the presence of this group of substances specific cryoprotective effect. OBJECTIVE: This research is aimed at studying the ability of pectins (zosteran, apple pectin АU-701, E-440) to change the freezing temperature of glycerol, which can increase the effect in the cryopreservation of nuclear cells. METHODS: Using the cryoscopic method we studied the ability of pectins (zosteran, apple pectin AU-701, E-440) to change the freezing temperature of glycerol...
November 2017: Cryo Letters
Jing Guo, Minmin Li, Yongguo Liu, Fengzhong Wang, Zhiqiang Kong, Yufeng Sun, Jia Lu, Nuo Jin, Yatao Huang, Jiameng Liu, Frédéric Francis, Bei Fan
Ultra-performance convergence chromatography is an environmentally-friendly analytical method that uses dramatically reduced amounts of organic solvents. In addition, a robust and highly sensitive chiral separation method was developed for the novel chiral acaricide cyflumetofen by using ultra-performance convergence chromatography coupled with tandem mass spectrometry, which shows that stereoisomer recoveries determined for various apple parts ranged from 78.3% to 119.9%, with the relative standard deviations being lower than 14...
May 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Maria Elena Alemán-Domínguez, Elena Giusto, Zaida Ortega, Maryam Tamaddon, Antonio Nizardo Benítez, Chaozong Liu
Microcrystalline cellulose (MCC) is proposed in this study as an additive in polycaprolactone (PCL) matrices to obtain three-dimensional (3D) printed scaffolds with improved mechanical and biological properties. Improving the mechanical behavior and the biological performance of polycaprolactone-based scaffolds allows to increase the potential of these structures for bone tissue engineering. Different groups of samples were evaluated in order to analyze the effect of the additive in the properties of the PCL matrix...
May 2, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Larissa Stebounova, Scott M Ebert, Logan T Murry, Christopher M Adams, Daryl J Murry
Ultra-performance liquid chromatography (UPLC) interfaced with atmospheric pressure chemical ionization mass-spectrometry was used to separate and quantify ursolic acid (UA) and oleanolic acid (OA) in human plasma. UA and OA were extracted from 0.5 mL human plasma using supported liquid extraction and separated utilizing an Acquity UPLC HSS column. The method has been validated for both UA and OA quantitation with a limit of detection of 0.5 ng/mL. The UPLC separations are carried out with isocratic elution with methanol and 5 mM ammonium acetate in water (85:15) as a mobile phase at a flow rate of 0...
April 26, 2018: Journal of Chromatographic Science
Michael Koerner, Eliza Fredette, Trudy A Watt, Peter Lloyd Jones
No abstract text is available yet for this article.
April 17, 2018: Annals of Internal Medicine
Pantea Rahmani Yeganeh, Jade Leahy, Schohraya Spahis, Natalie Patey, Yves Desjardins, Denis Roy, Edgard Delvin, Carole Garofalo, Jean-Philippe Leduc-Gaudet, David St-Pierre, Jean-François Beaulieu, André Marette, Gilles Gouspillou, Emile Levy
Inflammatory bowel diseases (IBDs) are multifaceted and relapsing immune disorders, which necessitate long-term dependence on powerful drugs. As the use of natural product-based therapies has emerged as a promising intervention, the present study aimed to further characterize dried apple peel powder (DAPP) mechanisms of action and evaluate the preventive and curative effects of DAPP on mitochondrial functions in a murine model. Induction of intestinal inflammation in mice is performed by oral administration of the dextran sodium sulfate (DSS) at 2...
March 21, 2018: Journal of Nutritional Biochemistry
Mostafa Mabrouk, Shaimaa A ElShebiney, Sayed H Kenawy, Gehan T El-Bassyouni, Esmat Ma Hamzawy
Copper (Cu)-doped calcium silicate nanoparticles were synthesized by a wet precipitation method as economical bone fracture filler. The aim was to improve the overall physicochemical properties, bioactivity, and biological performance of the bone fracture filler prepared herein. The synthesized nanoparticles were evaluated using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM). The bioactivity of the prepared nanoparticles was investigated after immersion in simulated body fluid (SBF) by means of inductively coupled plasma (ICP), SEM coupled with energy dispersive X-rays (EDX), and FTIR...
April 15, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Andrew T Wood, Dominique Everett, Sanjay Kumar, Manoj K Mishra, Vinoy Thomas
In the area of biomaterials, fibers not only offer increased mechanical response, but also serve as an extracellular matrix mimicking morphology to direct cellular attachment and proliferation. While biologically similar in morphology, soft, and flexible hydrogel materials have low mechanical properties. For applications in tissue engineering, the lack of directional cues and attachment regions within the biogels is undesired as cells require a guide for adequate attachment and organized proliferation. In this work, we have investigated the role of poly(lactic acid) (PLA) fiber length and concentration as a reinforcement phase in a gelatin hydrogel matrix and the resultant mechanical and cellular responses...
April 15, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Nana Sun, Yuan Ding, Zhexuan Tao, Hongjie You, Xiude Hua, Minghua Wang
An upconversion fluorescence DNA probe which consists of aptamer-conjugated magnet nanoparticles (apt-MNPs) and complementary DNA-conjugated upconversion nanoparticles (cDNA-UCNPs) was developed to detect acetamiprid. Acetamiprid can specifically conjugate with the apt-MNPs to dissociate the cDNA-UCNPs from the apt-MNPs and resulted in reduced fluorescence intensity through an external magnet. The change of fluorescence intensity (△I) is positively related to the concentration of acetamiprid, which can be applied for the quantification of acetamiprid...
August 15, 2018: Food Chemistry
Hanie Yousefi, M Monsur Ali, Hsuan-Ming Su, Carlos D M Filipe, Tohid F Didar
Here, we report the development of a transparent, durable, and flexible sensing surface that generates a fluorescence signal in the presence of a specific target bacterium. This material can be used in packaging, and it is capable of monitoring microbial contamination in various types of food products in real time without having to remove the sample or the sensor from the package. The sensor was fabricated by covalently attaching picoliter-sized microarrays of an E. coli-specific RNA-cleaving fluorogenic DNAzyme probe (RFD-EC1) to a thin, flexible, and transparent cyclo-olefin polymer (COP) film...
April 6, 2018: ACS Nano
Jared A Jaffey, Robert C Backus, Kaylyn M McDaniel, Amy E DeClue
Hypovitaminosis D has been extensively documented in critically ill humans. However, whether or not critically ill dogs have alterations in vitamin D concentrations remains unconfirmed. The primary aims of our study were to compare serum 25-hydroxycholecalciferol [25(OH)D] concentrations in critically ill dogs with healthy control dogs, determine the prognostic utility of serum 25(OH)D concentration as a biomarker in critically ill dogs, and to assess if serum 25(OH)D concentrations in critically ill dogs are associated with length of stay in the intensive care unit or illness severity...
2018: PloS One
Luanne R DeChristopher, Katherine L Tucker
There is growing evidence that intakes of high-fructose corn syrup (HFCS), HFCS-sweetened soda, fruit drinks and apple juice - a high-fructose 100 % juice - are associated with asthma, possibly because of the high fructose:glucose ratios and underlying fructose malabsorption, which may contribute to enteral formation of pro-inflammatory advanced glycation end products, which bind receptors that are mediators of asthma. Cox proportional hazards models were used to assess associations between intakes of these beverages and asthma risk, with data from the Framingham Offspring Cohort...
March 28, 2018: British Journal of Nutrition
Pedro Luiz Menin Ruiz, Bianca Andrade Handan, Carolina Foot Gomes de Moura, Livia Ribeiro Assis, Kelly Rossetti Fernandes, Ana Claudia Muniz Renno, Daniel Araki Ribeiro
The aim of this study was to investigate if grape or apple juices are able to protect bone tissue of rats exposed to cadmium. For this purpose, histopathological analysis and immunohistochemistry for RUNX-2 and RANK-L were investigated in this setting. A total of 20 adult Wistar rats were distributed into four groups (n = 5), as follows: control group, cadmium group, cadmium and grape juice group, and Cadmium and apple juice group. Control group received a single intraperitoneal (i.p.) water injection. Cadmium group received a single i...
March 26, 2018: Environmental Science and Pollution Research International
Andrew DeRouin, Roger Guillory, Weilue He, Megan Frost, Jeremy Goldman, Keat Ghee Ong
The gold standard of care for coronary artery disease, a leading cause of death for in the world, is balloon angioplasty in conjunction with stent deployment. However, implantation injuries and long-term presence of foreign material often promotes significant luminal tissue growth, leading to a narrowing of the artery and severely restricted blood flow. A promising method to mitigate this process is the use of biodegradable metallic stents, but thus far they have either degraded too slowly (iron) or disappeared prematurely (magnesium)...
March 23, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Anamarija Rogina, Maja Antunović, Dajana Milovac
Being a major component of bone tissue, hydroxyapatite is the most investigated calcium phosphate in the design and development of bone implants. The high brittleness and poor load-bearing properties have led researchers to manipulate hydroxyapatite performance by applying polymer or metal materials. The present study focuses on biomimetic approach of the hydroxyapatite synthesis from the cuttlefish bone in order to preserve highly porous structure. The low stiffness of hydroxyapatite scaffold was altered by thin polycaprolactone/poly(lactic acid) coating, resulting in remarkably 18-fold increase of Young's modulus...
March 23, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
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