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https://www.readbyqxmd.com/read/27853868/immune-function-monitoring-in-lung-transplantation-using-adenosine-triphosphate-production-time-trends-and-relationship-to-postoperative-infection
#1
Mamoru Takahashi, Akihiro Ohsumi, Keiji Ohata, Takeshi Kondo, Hideki Motoyama, Kyoko Hijiya, Akihiro Aoyama, Hiroshi Date, Toyofumi F Chen-Yoshikawa
PURPOSE: The ImmuKnow (IK) assay is a comprehensive immune function test that involves measuring adenosine triphosphate produced by the cluster of differentiation 4+ T lymphocytes in peripheral blood. The aim of this study was to analyze the time trends of IK values and assess the relationship between IK values and infections in lung transplants. METHODS: We prospectively collected 178 blood samples from 22 deceased-donor lung transplant (DDLT) recipients and 17 living-donor lobar lung transplant (LDLLT) recipients...
November 16, 2016: Surgery Today
https://www.readbyqxmd.com/read/27783145/infectious-agents-after-liver-transplant-etiology-timeline-and-patients-cell-mediated-immunity-responses
#2
Angela Chiereghin, Evangelia Petrisli, Matteo Ravaioli, Maria Cristina Morelli, Gabriele Turello, Diego Squarzoni, Giulia Piccirilli, Simone Ambretti, Liliana Gabrielli, Antonio Daniele Pinna, Maria Paola Landini, Tiziana Lazzarotto
Infections continue to be one of the leading causes of morbidity and mortality in liver transplant recipients. We retrospectively reviewed the symptomatic infectious episodes that occurred during the first year post-transplant to determine time of onset, causative pathogens and cell-mediated immunity response patterns. Ninety-eight of the 202 (48.5%) recipients enrolled developed at least one infectious episode. The total number of infectious episodes was 135: 77 (57.1%) bacterial, 45 (33.3%) viral and 13 (9...
October 25, 2016: Medical Microbiology and Immunology
https://www.readbyqxmd.com/read/27569951/correlation-between-immunosuppressive-therapy-and-cd4-t-cell-intracellular-adenosine-triphosphate-levels-in-liver-transplant-recipients
#3
W Qu, Z-J Zhu, L-Y Sun, L Wei, Y Liu, Z-G Zeng
OBJECTIVE: The aim of this work was to analyze the correlation between immunosuppressive therapy and CD4(+) T-cell intracellular adenosine triphosphate (iATP) levels after liver transplantation and to describe the distribution characteristics of iATP in CD4(+) T cells among liver transplant recipients. METHODS: We studied 172 patients who were followed regularly after liver transplantation with long-term stable conditions from July 2010 to October 2012. CD4(+) T-cell iATP levels were detected with the use of the Cylex Immuknow Assay method and analyzed retrospectively according to immunosuppressive therapy protocol...
July 2016: Transplantation Proceedings
https://www.readbyqxmd.com/read/27500258/lowered-immune-cell-function-in-liver-recipients-recovered-from-posttransplant-lymphoproliferative-disease-who-developed-graft-tolerance
#4
Patrick Ho Yu Chung, See Ching Chan, Kwong Leung Chan, Yuk Sing Chan, Janette Siu Yin Kwok, Chung Mau Lo
UNLABELLED: Tolerance after treatment and recovery from posttransplant lymphoproliferative disease (PTLD) have been described but little is known about the immunology. The objective of this study is to evaluate the immunity of pediatric recipients who recovered from PTLD. MATERIALS AND METHODS: Pediatric recipients who recovered from PTLD after liver transplant and twice the number of recipients who never had PTLD were recruited. Their immune statuses were measured by ImmuKnow (measurement of adenosine 5-triphospate level produced CD4+ T helper cells), and the results were divided into 3 groups, "low" (≤225 ng/mL), "moderate" (226 to 524 ng/mL), and "high" (≥525 ng/mL)...
March 2016: Transplantation Direct
https://www.readbyqxmd.com/read/27197999/peripheral-blood-cd4-cell-atp-activity-measurement-to-predict-hcc-recurrence-post-dcd-liver-transplant
#5
W Zhang, H Zhong, L Zhuang, J Yu, X Xu, W Wang, M Zhang, L Zhou, S Zheng
BACKGROUND AND AIM: Hepatocellular carcinoma (HCC) recurrence after orthotopic liver transplantation (OLT) continues to confound transplant surgeons and physicians. There are no effective methods to predict the patients at risk for recurrence so far although many studies have sought meaningful biomarkers. The ImmuKnow (IMK) assay is an immune cell function assay that detects cell-mediated immunity in an immunosuppressed population, mainly measuring peripheral blood CD4(+) adenosine triphosphate (ATP) release...
June 2016: International Journal of Clinical Practice
https://www.readbyqxmd.com/read/27095000/clinical-utility-of-cd4-function-assessment-viracor-ibt-immuknow-test-in-lung-recipients
#6
Davide Piloni, Sara Magni, Tiberio Oggionni, Alberto Benazzo, Giulia Stella, Luigia Scudeller, Monica Morosini, Emanuela Cova, Federica Meloni
The ImmuKnow assay measures cell-mediated immunity, quantifying ATP production from peripheral blood CD4+T-cells in solid-organ transplant patients who undergo immunosuppressive therapy. We aimed to measure functional immunity in lung transplant recipients and correlate Immuknow values with immunosuppression levels, presence of chronic lung allograft dysfunction (CLAD) and infections. We evaluated 61 lung recipients who underwent follow-up for lung transplantation between 2010 and 2014. Rejection and infection were retrospectively analyzed...
July 2016: Transplant Immunology
https://www.readbyqxmd.com/read/26693232/in-vitro-immune-cell-monitoring-as-a-guide-for-long-term-immunosuppression-in-adult-liver-transplant-recipients
#7
Eunkyoung Jwa, Shin Hwang, Yong-Jae Kwon, Nayoung Kim, Gi-Won Song, Dong-Hwan Jung, Chul-Soo Ahn, Eunyoung Tak, Deok-Bog Moon, Ki-Hun Kim, Tae-Yong Ha, Gil-Chun Park, Sung-Gyu Lee
BACKGROUNDS/AIMS: We evaluated the clinical usability of immune cell monitoring in adult liver transplantation (LT) recipients. METHODS: This study was composed of two parts as using calcineurin phosphatase (CNP) activity assay and ImmuKnow assay independently as in vitro monitoring tools of immune cell function in adult LT recipients. RESULTS: There was a rough correlation between CNP activity and tacrolimus concentration in 33 patients. This association was evident in patients who were only administered tacrolimus, but disappeared after the co-administration of mycophenolate...
November 2015: Korean Journal of Hepato-biliary-pancreatic Surgery
https://www.readbyqxmd.com/read/26667109/relationship-between-immune-function-recovery-and-infectious-complications-in-patients-following-living-donor-liver-transplantation
#8
Shinichiro Kobayashi, Akihiko Soyama, Mitsuhisa Takatsuki, Masaaki Hidaka, Tomohiko Adachi, Amane Kitasato, Ayaka Kinoshita, Takanobu Hara, Kengo Kanetaka, Fumihiko Fujita, Tamotsu Kuroki, Susumu Eguchi
AIM: The ImmuKnow (IK) assay enables the evaluation of peripheral blood CD4(+) adenosine triphosphate activity to facilitate an objective assessment of the cellular immune function in immunosuppressed patients. However, it is unclear whether the IK assay is utilized during the acute postoperative periods following living donor liver transplantation (LDLT). METHODS: The IK values of 43 LDLT recipients were measured during the month following LDLT to evaluate the relationship between the measured IK values and infectious events...
August 2016: Hepatology Research: the Official Journal of the Japan Society of Hepatology
https://www.readbyqxmd.com/read/26622423/impact-of-perioperative-probiotic-treatment-for-surgical-site-infections-in-patients-with-colorectal-cancer
#9
Naoya Aisu, Shu Tanimura, Yuichi Yamashita, Kanefumi Yamashita, Kenji Maki, Yoichiro Yoshida, Takamitsu Sasaki, Shinsuke Takeno, Seiichiro Hoshino
The aim of the present study was to estimate the effect of the perioperative administration of probiotics in patients undergoing colorectal cancer (CRC) surgery. The study focused on a total of 156 consecutive surgeries carried out from among all the elective CRC surgeries performed between April 2009 and March 2013. The patients involved in surgeries undertaken between April 2009 and October 2011 were placed in the non-probiotic group (group A, 81 patients) and those involved in surgeries between November 2011 and March 2013 were placed in the probiotic group (group B, 75 patients)...
September 2015: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/26436395/lack-of-clinical-significance-of-the-immuknow-tm-cylex-assay-for-the-detection-of-cellular-immune-function-in-patients-with-renal-cell-carcinoma
#10
K Zheng, J P Zhang, J M Tan, W Z Wu, S L Yang, D D Ke
This study aimed to explore the clinical value of the CD4(+) T cell ATP levels in patients with renal cell carcinoma through the application of the ImmuKnow(TM)-Cylex(®) assay. We recruited 104 patients with renal cancer who had undergone surgery at Fuzhou General Hospital from March 2009 to June 2012, and were subsequently treated by dendritic cell and cytokine-induced killer cell bio-therapy or interferon-α therapy. The changes in CD4(+) T cell ATP levels were detected at the perioperative period and at 10 days, 1 month, 3 months, and 1 year after the surgery using the ImmuKnow assay...
2015: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/26381574/cd4-t-cell-function-assay-using-cylex-immuknow-and-lymphocyte-subset-recovery-following-allogeneic-hematopoietic-stem-cell-transplantation
#11
Yongjun Jo, Jihyang Lim, Yonggoo Kim, Kyungja Han, Woo-Sung Min, Eun-Jee Oh
We analyzed CD4 T-lymphocytes ATP levels along with lymphocyte subsets in 160 samples from 111 post-allogeneic hematopoietic stem cell transplantation (alloHSCT) patients. In patients with stable status, ImmuKnow levels changed over time and the 6-month post-alloHSCT levels were significantly higher than those tested within 6 months post-alloHSCT (P < 0.001). Although, ImmuKnow levels for acute graft-versus-host disease (GVHD) or infection episodes were not significantly different compared to those for stable alloHSCT, the levels were correlated with specific lymphocyte subpopulations at different times; the results within 6 months post-alloHSCT showed low positive correlation with natural killer cell count (r = 0...
October 2015: Transplant Immunology
https://www.readbyqxmd.com/read/26155107/serial-immuknow-assay-in-stable-kidney-transplant-recipients
#12
Hyung Hwan Moon, Tae-Seok Kim, Sanghoon Lee, Sanghyun Song, Milljae Shin, Jae Berm Park, Jong Man Kim, Hye Ryoun Jang, Wooseong Huh, Jae-Won Joh, Sung Joo Kim
OBJECTIVES: The ImmuKnow assay (cylex Inc., Columbia, MD) has been reported to measure the global immune monitoring tool for organ transplantation recipients. We assess immuKnow ATP values in stable kidney transplant patients. MATERIAL AND METHODS: Patients who were kidney transplanted between September 2008 and May 2011 were enrolled in the prospective serial ImmuKnow assay study. The criteria of inclusion were living donor kidney transplantation (KT), no evidence of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection, and PRA less than 50%...
2014: Central-European Journal of Immunology
https://www.readbyqxmd.com/read/26005939/-immuknow-and-long-term-kidney-graft
#13
Cristina Andreotti, Michela Zortea, Alessandro Provenzani, Maria Gentilini, Nadia Bucella
INTRODUCTION: The survival of transplanted kidneys has improved over time, but there is an increased risk of neoplastic disease. In the long time follow up, non-melanoma skin cancers (NMSC) are the most frequent diseases and at the time of the occurrence of a NMSC we should evaluate a reduction or a change of IS. From a clinical point of view, the evaluation of immunosuppression is still a problem. The Immuknow assay may be of help in evaluating the immune response of transplanted patients...
March 2015: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
https://www.readbyqxmd.com/read/25757214/immunosuppression-modifications-based-on-an-immune-response-assay-results-of-a-randomized-controlled-trial
#14
RANDOMIZED CONTROLLED TRIAL
Matteo Ravaioli, Flavia Neri, Tiziana Lazzarotto, Valentina Rosa Bertuzzo, Paolo Di Gioia, Giacomo Stacchini, Maria Cristina Morelli, Giorgio Ercolani, Matteo Cescon, Angela Chiereghin, Massimo Del Gaudio, Alessandro Cucchetti, Antonio D Pinna
BACKGROUND: An immune function assay shows promise for identifying solid organ recipients at risk for infection or rejection. The following randomized prospective study was designed to assess the clinical benefits of adjusting immunosuppressive therapy in liver recipients based on immune function assay results. METHODS: Adult liver recipients were randomized to standard practice (control group; n = 102) or serial immune function testing (interventional group; n = 100) performed with a commercially available in vitro diagnostic assay (ImmuKnow; Viracor-IBT Laboratories, Lee's Summit, MO) before transplantation, immediately after surgery and at day 1, weeks 1 to 4, 6, and 8, and months 3 to 6, 9, and 12...
August 2015: Transplantation
https://www.readbyqxmd.com/read/25549254/relationship-between-the-peripheral-lymphocyte-response-to-mycophenolic-acid-in-vitro-and-the-level-of-atp-in-peripheral-cd4-lymphocytes-before-and-after-renal-transplantation
#15
K Sugiyama, M Tsukaguchi, H Sasahara, K Isogai, A Toyama, H Satoh, K Saito, Y Nakagawa, K Takahashi, S Tanaka, K Onda, T Hirano
OBJECTIVE: The lymphocyte immunosuppressant sensitivity test has been used to predict the pharmacodynamics of immunosuppressive drugs for the purpose of preventing acute rejection and infection after renal transplantation. On the other hand, measuring the ATP levels in peripheral CD4+ lymphocytes is also able to monitor the risks of rejection and infection in transplant recipients. In the present study, we examined the relationship between the mycophenolic acid pharmacodynamics and the ATP levels in peripheral lymphocytes before and after renal transplantation...
December 2015: Drug Research
https://www.readbyqxmd.com/read/25498049/performance-of-the-immuknow-assay-in-differentiating-infection-and-acute-rejection-after-kidney-transplantation-a-meta-analysis
#16
REVIEW
Z Wang, X Liu, P Lu, Z Han, J Tao, J Wang, K Liu, B Wu, C Yin, R Tan, M Gu
BACKGROUND: The ImmuKnow test is an assay for determining the functional activity of immunocytes, which reflects cell-mediated immune responses in populations undergoing organ transplantation. METHODS: Electronic and manual searches were conducted to identify studies of the ImmuKnow test. After methodological quality assessment and data extraction, the pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were assessed, and summary receiver operating characteristic analysis was performed systematically...
December 2014: Transplantation Proceedings
https://www.readbyqxmd.com/read/25475045/monitoring-t-cell-alloreactivity
#17
REVIEW
Anita Mehrotra, Jeremy Leventhal, Carolina Purroy, Paolo Cravedi
Currently, immunosuppressive therapy in kidney transplant recipients is center-specific, protocol-driven, and adjusted according to functional or histological evaluation of the allograft and/or signs of drug toxicity or infection. As a result, a large fraction of patients receive too much or too little immunosuppression, exposing them to higher rates of infection, malignancy and drug toxicity, or increased risk of acute and chronic graft injury from rejection, respectively. The individualization of immunosuppression requires the development of assays able to reliably quantify and/or predict the magnitude of the recipient's immune response toward the allograft...
April 2015: Transplantation Reviews
https://www.readbyqxmd.com/read/25469274/cd4-t-lymphocytes-are-activated-by-surgical-stress-following-colorectal-resection-in-cancer-patients
#18
Kenji Maki, Shinsuke Takeno, Naoya Aisu, Kanefumi Yamashita, Masayasu Naito, Seiichiro Hoshino, Yuichi Yamashita
The aim of the present study was to measure adenosine triphosphate (ATP) levels in CD4(+) T cells as a marker of T-cell activity following surgery for colorectal cancer using the ImmuKnow assay kit. A total of 16 consecutive patients who underwent surgical resection for colorectal cancer between August and December, 2012 were enrolled in this study, of whom 7 underwent laparoscopic resection and 9 underwent open abdominal surgery. The intracellular ATP levels in CD4(+) T-lymphocytes were measured using the ImmuKnow assay kit preoperatively and on the 1st, 4th and 8th postoperative days, as were the white blood cell (WBC) count, lymphocyte count and C-reactive protein (CRP) levels...
January 2015: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/25418834/evaluation-of-the-immune-function-assay-in-pediatric-living-donor-liver-transplantation
#19
Akinari Fukuda, Ken-Ichi Imadome, Seisuke Sakamoto, Takanobu Shigeta, Hajime Uchida, Masatoshi Matsunami, Kengo Sasaki, Hiroyuki Kanazawa, Fuyuko Kawano, Atsuko Nakazawa, Shigeyoshi Fujiwara, Mureo Kasahara
The immune function (ImmuKnow) assay is a measure of cell-mediated immunity based on the peripheral CD4+ T cell ATP activity. The efficacy of ImmuKnow in pediatric LDLT is not well documented. The aim of this study was to assess the correlations between the ImmuKnow and the clinical status in pediatric LDLT recipients. A total of 716 blood samples were obtained from 60 pediatric LDLT recipients (one month to 16 yr of age). The recipient's status was classified as follows: stable, infection, or rejection. The ImmuKnow values in the pediatric LDLT recipients with a clinically stable status had a lower immune response (IQR 85-297 ATP ng/mL) than that previously reported in adults...
March 2015: Pediatric Transplantation
https://www.readbyqxmd.com/read/25369255/-correlation-analysis-of-survival-period-and-cd4-tcell-iatp-levels-in-liver-transplant-recipients
#20
Wei Qu, Liying Sun, Zhijun Zhu, Ying Liu, Lin Wei, Zhigui Zeng
OBJECTIVE: To analyze the correlation between survival time after liver transplantation and the intracellular (i)ATP levels of CD4+ T cells. METHODS: Liver transplantation patients treated in our hospital,and with complete follow-up data,were enrolled retrospectively in our study (between July 2010 to October 2012) and divided into six groups according to survival time: less than 1 year,1-2 years,2-3 years,3-4 years,4-8 years,and more than 8 years.The less than 1 year survival group was further sub-divided for survival less than 1 month,1-2 months,2-3 months,3-5 months and 5-12 months...
September 2014: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
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