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Ying Luo, Wen-Bin Ji, Wei-Dong Duan, Xian-Jie Shi, Zhi-Ming Zhao
BACKGROUND: The manipulation of immunosuppression therapy remains challenging in patients who develop infectious diseases or multiple organ dysfunction after liver transplantation. We evaluated the outcomes of delayed introduction of immunosuppression in the patients after liver transplantation under immune monitoring with ImmuKnow assay. METHODS: From March 2009 to February 2014, 225 consecutive liver recipients in our institute were included. The delayed administration of immunosuppressive regimens was attempted in 11 liver recipients with multiple severe comorbidities...
October 15, 2017: Hepatobiliary & Pancreatic Diseases International: HBPD INT
X Chen, Y Y Yu, Z Y Dai, C M Xia, D Li, H F Yan
No abstract text is available yet for this article.
January 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
Junichi Kusano, Rei Kato, Hiroto Matsuda, Yoshikazu Hara, Yoshiaki Fujii, Shinya Suzuki, Masao Sekiyama, Eiki Ando, Kentaro Sugiyama, Toshihiko Hirano
The ATP monitoring assay is a useful biomarker for risk monitoring to detect infection and rejection episodes in transplant recipients. Hemodialysis patients have a higher rate of infectious mortality. Infections in hemodialysis patients are mainly caused by venous catheters, uremia, malnutrition and inflammation. However, the risk of infection episodes has not been evaluated using a lymphocyte ATP monitoring assay in hemodialysis and chronic kidney disease (CKD) patients. We measured the ATP amounts in the peripheral CD4+ cells of CKD (N = 85) and dialysis patients (N = 17) using an "Immuknow" assay kit...
March 13, 2017: Therapeutic Apheresis and Dialysis
W Qu, Z-J Zhu, L-Y Sun, L Wei, Y Liu, Z-G Zeng
OBJECTIVE: The objectives of this study were to analyze the potential correlation between post-liver transplantation survival interval and CD4(+) T-cell intracellular ATP (iATP) levels, and to describe the distribution of CD4(+) T-cell iATP levels in liver transplant recipients. METHODS: This was a retrospective analysis of clinical data of 273 patients who underwent liver transplantation from July 2010 to October 2012 in our center and achieved long-term stable survival...
March 2017: Transplantation Proceedings
Mamoru Takahashi, Akihiro Ohsumi, Keiji Ohata, Takeshi Kondo, Hideki Motoyama, Kyoko Hijiya, Akihiro Aoyama, Hiroshi Date, Toyofumi F Chen-Yoshikawa
PURPOSE: The ImmuKnow (IK) assay is a comprehensive immune function test that involves measuring adenosine triphosphate produced by the cluster of differentiation 4+ T lymphocytes in peripheral blood. The aim of this study was to analyze the time trends of IK values and assess the relationship between IK values and infections in lung transplants. METHODS: We prospectively collected 178 blood samples from 22 deceased-donor lung transplant (DDLT) recipients and 17 living-donor lobar lung transplant (LDLLT) recipients...
June 2017: Surgery Today
Angela Chiereghin, Evangelia Petrisli, Matteo Ravaioli, Maria Cristina Morelli, Gabriele Turello, Diego Squarzoni, Giulia Piccirilli, Simone Ambretti, Liliana Gabrielli, Antonio Daniele Pinna, Maria Paola Landini, Tiziana Lazzarotto
Infections continue to be one of the leading causes of morbidity and mortality in liver transplant recipients. We retrospectively reviewed the symptomatic infectious episodes that occurred during the first year post-transplant to determine time of onset, causative pathogens and cell-mediated immunity response patterns. Ninety-eight of the 202 (48.5%) recipients enrolled developed at least one infectious episode. The total number of infectious episodes was 135: 77 (57.1%) bacterial, 45 (33.3%) viral and 13 (9...
February 2017: Medical Microbiology and Immunology
W Qu, Z-J Zhu, L-Y Sun, L Wei, Y Liu, Z-G Zeng
OBJECTIVE: The aim of this work was to analyze the correlation between immunosuppressive therapy and CD4(+) T-cell intracellular adenosine triphosphate (iATP) levels after liver transplantation and to describe the distribution characteristics of iATP in CD4(+) T cells among liver transplant recipients. METHODS: We studied 172 patients who were followed regularly after liver transplantation with long-term stable conditions from July 2010 to October 2012. CD4(+) T-cell iATP levels were detected with the use of the Cylex Immuknow Assay method and analyzed retrospectively according to immunosuppressive therapy protocol...
July 2016: Transplantation Proceedings
Patrick Ho Yu Chung, See Ching Chan, Kwong Leung Chan, Yuk Sing Chan, Janette Siu Yin Kwok, Chung Mau Lo
UNLABELLED: Tolerance after treatment and recovery from posttransplant lymphoproliferative disease (PTLD) have been described but little is known about the immunology. The objective of this study is to evaluate the immunity of pediatric recipients who recovered from PTLD. MATERIALS AND METHODS: Pediatric recipients who recovered from PTLD after liver transplant and twice the number of recipients who never had PTLD were recruited. Their immune statuses were measured by ImmuKnow (measurement of adenosine 5-triphospate level produced CD4+ T helper cells), and the results were divided into 3 groups, "low" (≤225 ng/mL), "moderate" (226 to 524 ng/mL), and "high" (≥525 ng/mL)...
March 2016: Transplantation Direct
W Zhang, H Zhong, L Zhuang, J Yu, X Xu, W Wang, M Zhang, L Zhou, S Zheng
BACKGROUND AND AIM: Hepatocellular carcinoma (HCC) recurrence after orthotopic liver transplantation (OLT) continues to confound transplant surgeons and physicians. There are no effective methods to predict the patients at risk for recurrence so far although many studies have sought meaningful biomarkers. The ImmuKnow (IMK) assay is an immune cell function assay that detects cell-mediated immunity in an immunosuppressed population, mainly measuring peripheral blood CD4(+) adenosine triphosphate (ATP) release...
June 2016: International Journal of Clinical Practice
Davide Piloni, Sara Magni, Tiberio Oggionni, Alberto Benazzo, Giulia Stella, Luigia Scudeller, Monica Morosini, Emanuela Cova, Federica Meloni
The ImmuKnow assay measures cell-mediated immunity, quantifying ATP production from peripheral blood CD4+T-cells in solid-organ transplant patients who undergo immunosuppressive therapy. We aimed to measure functional immunity in lung transplant recipients and correlate Immuknow values with immunosuppression levels, presence of chronic lung allograft dysfunction (CLAD) and infections. We evaluated 61 lung recipients who underwent follow-up for lung transplantation between 2010 and 2014. Rejection and infection were retrospectively analyzed...
July 2016: Transplant Immunology
Eunkyoung Jwa, Shin Hwang, Yong-Jae Kwon, Nayoung Kim, Gi-Won Song, Dong-Hwan Jung, Chul-Soo Ahn, Eunyoung Tak, Deok-Bog Moon, Ki-Hun Kim, Tae-Yong Ha, Gil-Chun Park, Sung-Gyu Lee
BACKGROUNDS/AIMS: We evaluated the clinical usability of immune cell monitoring in adult liver transplantation (LT) recipients. METHODS: This study was composed of two parts as using calcineurin phosphatase (CNP) activity assay and ImmuKnow assay independently as in vitro monitoring tools of immune cell function in adult LT recipients. RESULTS: There was a rough correlation between CNP activity and tacrolimus concentration in 33 patients. This association was evident in patients who were only administered tacrolimus, but disappeared after the co-administration of mycophenolate...
November 2015: Korean Journal of Hepato-biliary-pancreatic Surgery
Shinichiro Kobayashi, Akihiko Soyama, Mitsuhisa Takatsuki, Masaaki Hidaka, Tomohiko Adachi, Amane Kitasato, Ayaka Kinoshita, Takanobu Hara, Kengo Kanetaka, Fumihiko Fujita, Tamotsu Kuroki, Susumu Eguchi
AIM: The ImmuKnow (IK) assay enables the evaluation of peripheral blood CD4(+) adenosine triphosphate activity to facilitate an objective assessment of the cellular immune function in immunosuppressed patients. However, it is unclear whether the IK assay is utilized during the acute postoperative periods following living donor liver transplantation (LDLT). METHODS: The IK values of 43 LDLT recipients were measured during the month following LDLT to evaluate the relationship between the measured IK values and infectious events...
August 2016: Hepatology Research: the Official Journal of the Japan Society of Hepatology
Naoya Aisu, Shu Tanimura, Yuichi Yamashita, Kanefumi Yamashita, Kenji Maki, Yoichiro Yoshida, Takamitsu Sasaki, Shinsuke Takeno, Seiichiro Hoshino
The aim of the present study was to estimate the effect of the perioperative administration of probiotics in patients undergoing colorectal cancer (CRC) surgery. The study focused on a total of 156 consecutive surgeries carried out from among all the elective CRC surgeries performed between April 2009 and March 2013. The patients involved in surgeries undertaken between April 2009 and October 2011 were placed in the non-probiotic group (group A, 81 patients) and those involved in surgeries between November 2011 and March 2013 were placed in the probiotic group (group B, 75 patients)...
September 2015: Experimental and Therapeutic Medicine
K Zheng, J P Zhang, J M Tan, W Z Wu, S L Yang, D D Ke
This study aimed to explore the clinical value of the CD4(+) T cell ATP levels in patients with renal cell carcinoma through the application of the ImmuKnow(TM)-Cylex(®) assay. We recruited 104 patients with renal cancer who had undergone surgery at Fuzhou General Hospital from March 2009 to June 2012, and were subsequently treated by dendritic cell and cytokine-induced killer cell bio-therapy or interferon-α therapy. The changes in CD4(+) T cell ATP levels were detected at the perioperative period and at 10 days, 1 month, 3 months, and 1 year after the surgery using the ImmuKnow assay...
2015: Genetics and Molecular Research: GMR
Yongjun Jo, Jihyang Lim, Yonggoo Kim, Kyungja Han, Woo-Sung Min, Eun-Jee Oh
We analyzed CD4 T-lymphocytes ATP levels along with lymphocyte subsets in 160 samples from 111 post-allogeneic hematopoietic stem cell transplantation (alloHSCT) patients. In patients with stable status, ImmuKnow levels changed over time and the 6-month post-alloHSCT levels were significantly higher than those tested within 6 months post-alloHSCT (P < 0.001). Although, ImmuKnow levels for acute graft-versus-host disease (GVHD) or infection episodes were not significantly different compared to those for stable alloHSCT, the levels were correlated with specific lymphocyte subpopulations at different times; the results within 6 months post-alloHSCT showed low positive correlation with natural killer cell count (r = 0...
October 2015: Transplant Immunology
Hyung Hwan Moon, Tae-Seok Kim, Sanghoon Lee, Sanghyun Song, Milljae Shin, Jae Berm Park, Jong Man Kim, Hye Ryoun Jang, Wooseong Huh, Jae-Won Joh, Sung Joo Kim
OBJECTIVES: The ImmuKnow assay (cylex Inc., Columbia, MD) has been reported to measure the global immune monitoring tool for organ transplantation recipients. We assess immuKnow ATP values in stable kidney transplant patients. MATERIAL AND METHODS: Patients who were kidney transplanted between September 2008 and May 2011 were enrolled in the prospective serial ImmuKnow assay study. The criteria of inclusion were living donor kidney transplantation (KT), no evidence of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection, and PRA less than 50%...
2014: Central-European Journal of Immunology
Cristina Andreotti, Michela Zortea, Alessandro Provenzani, Maria Gentilini, Nadia Bucella
INTRODUCTION: The survival of transplanted kidneys has improved over time, but there is an increased risk of neoplastic disease. In the long time follow up, non-melanoma skin cancers (NMSC) are the most frequent diseases and at the time of the occurrence of a NMSC we should evaluate a reduction or a change of IS. From a clinical point of view, the evaluation of immunosuppression is still a problem. The Immuknow assay may be of help in evaluating the immune response of transplanted patients...
March 2015: Giornale Italiano di Nefrologia: Organo Ufficiale Della Società Italiana di Nefrologia
Matteo Ravaioli, Flavia Neri, Tiziana Lazzarotto, Valentina Rosa Bertuzzo, Paolo Di Gioia, Giacomo Stacchini, Maria Cristina Morelli, Giorgio Ercolani, Matteo Cescon, Angela Chiereghin, Massimo Del Gaudio, Alessandro Cucchetti, Antonio D Pinna
BACKGROUND: An immune function assay shows promise for identifying solid organ recipients at risk for infection or rejection. The following randomized prospective study was designed to assess the clinical benefits of adjusting immunosuppressive therapy in liver recipients based on immune function assay results. METHODS: Adult liver recipients were randomized to standard practice (control group; n = 102) or serial immune function testing (interventional group; n = 100) performed with a commercially available in vitro diagnostic assay (ImmuKnow; Viracor-IBT Laboratories, Lee's Summit, MO) before transplantation, immediately after surgery and at day 1, weeks 1 to 4, 6, and 8, and months 3 to 6, 9, and 12...
August 2015: Transplantation
K Sugiyama, M Tsukaguchi, H Sasahara, K Isogai, A Toyama, H Satoh, K Saito, Y Nakagawa, K Takahashi, S Tanaka, K Onda, T Hirano
OBJECTIVE: The lymphocyte immunosuppressant sensitivity test has been used to predict the pharmacodynamics of immunosuppressive drugs for the purpose of preventing acute rejection and infection after renal transplantation. On the other hand, measuring the ATP levels in peripheral CD4+ lymphocytes is also able to monitor the risks of rejection and infection in transplant recipients. In the present study, we examined the relationship between the mycophenolic acid pharmacodynamics and the ATP levels in peripheral lymphocytes before and after renal transplantation...
December 2015: Drug Research
Z Wang, X Liu, P Lu, Z Han, J Tao, J Wang, K Liu, B Wu, C Yin, R Tan, M Gu
BACKGROUND: The ImmuKnow test is an assay for determining the functional activity of immunocytes, which reflects cell-mediated immune responses in populations undergoing organ transplantation. METHODS: Electronic and manual searches were conducted to identify studies of the ImmuKnow test. After methodological quality assessment and data extraction, the pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were assessed, and summary receiver operating characteristic analysis was performed systematically...
December 2014: Transplantation Proceedings
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